Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,821,527	A→G	intergenic (+26600/‑112804)	unknown → / → unknown	tRNA‑Leu‑CAA/tRNA‑Asp‑GTC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,821,527	0	A	G	100.0%	192.4 / NA	74	intergenic (+26600/‑112804)	unknown/unknown	tRNA‑Leu‑CAA/tRNA‑Asp‑GTC
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (43/31);  total (43/31)

CCTCTTTTCACTTTTTCCACATTTCTAAATAAACGATACACCCCGATTAAATCTTTTTTAGCAATATATTTAAACGGCAATAATAAATAATACAAAAAACGATAATGGCATACGGAAGACATCATATCCTGCCACTCTTGAGGTTCACAATCCTGTTCATAACCGCGCATAACCGTGCGGACAGTTTCATCGGAAATATTTTCCTCACGACATAAATTATAAATAAACAAAATCAAATCACGCACTTTCTGACGAGGTAAGTTCCGTTTTGTTGCGTCCACTTCAAAGTCAAT‑AAATAACACCCTGCCGTCATGCCATAAAATATCCCGAATCGCCGGGCGCCCATGCACCAATCCCTGTTGATGTAACTGCGCCAAGGCTTTAGCACAATCCAGCAGAATCTGTTGTTTGGTTTGCGTTGGGATATTATCGTCAACCCAATTTGCTACGCTTTTGCCACCATCTTCCAGCACCAGATAATCT  >  1/1821288‑1821770                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                     ccTCTTTTCACTTTTTCCACATTTCTAAATAAACGATACACCCCGATTAAATCTTTTTTAGCAATATATTTAAACGGCAATAATAAATAATACAAAAAACGATAATGGCATACGGAAGACATCATATCCTGCCACTCTTGAGGTTCACAATCCTGTTCATAACCGCGCATAACCGTGCGGACAGTTTCATCGGAAATATTTTCCTCACGACATAAATTATAAATAAACAAAATCAAATCGCGCACttt                                                                                                                                                                                                                                              >  1:710603/1‑248 (MQ=255)    ctTTTCACTTTTTCCACATTTCTAAATAAACGATACACCCCGATTAAATCTTTTTTAGCAATATATTTAAACGGCAATAATAAATAATACAAAAAACGATAATGGCATACGGAAGACATCATATCCTGCCACTCTTGAGGTTCACAATCCTGTTCATAACCGCGCATAACCGTGCGGACAGTTTCATCGGAAATATTTTCCTCACGACATAAATTATAAATAAACAAAATCAAATCGCGCACTTTCTGACg                                                                                                                                                                                                                                        >  1:196599/1‑251 (MQ=255)                          taaataaaCGATACACCCCGATTAAATCTTTTTTAGCAATATATTTAAACGGCAATAATAAATAATACAAAAAACGATAATGGCATACGGAAGACATCATATCCTGCCACTCTTGAGGTTCACAATCCTGTTCATAACCGCGCATAACCGTGCGGACAGTTTCATCGGAAATATTTTCCTCACGACATAAATTATAAATAAACAAAATCAAATCGCGCACTTTCTgctgcgtggtggcaattagtctgctg                                                                                                                                                                                                                  <  1:88130/251‑26 (MQ=255)                               aaaCGATACACCCCGATTAAATCTTTTTTAGCAATATATTTAAACGGCAATAATAAATAATACAAAAAACGATAATGGCATACGGAAGACATCATATCCTGCCACTCTTGAGGTTCACAATCCTGTTCATAACCGCGCATAACCGTGCGGACAGTTTCATCGGAAATATTTTCCTCACGACATAAATTATAAATAAACAAAATCAAATCGCGCACTTTCTGACGAGGTAAGTTCCGTTTTGTTGCGTCCa                                                                                                                                                                                                              >  1:62842/1‑250 (MQ=255)                                    aTACACCCCGATTAAATCTTTTTTAGCAATATATTTAAACGGCAATAATAAATAATACAAAAAACGATAATGGCATACGGAAGACATCATATCCTGCCACTCTTGAGGTTCACAATCCTGTTCATAACCGCGCATAACCGTGCGGACAGTTTCATCGGAAATATTTTCCTCACGACATAAATTATAAATAAACAAAATCAAATCGCGCACTTTCTGACGAGGTAAGTTCCGTTTTGTTGCGTCCACTTCaa                                                                                                                                                                                                        >  1:60518/1‑251 (MQ=255)                                             gATTAAATCTTTTTTAGCAATATATTTAAACGGCAATAATAAATAATACAAAAAACGATAATGGCATACGGAAGACATCATATCCTGCCACTCTTGAGGTTCACAATCCTGTTCATAACCGCGCATAACCGTGCGGACAGTTTCATCGGAAATATTTTCCTCACGACATCAATTATAAATAAACAAAATCAAATCGCGCACTTTCTGAc                                                                                                                                                                                                                                         >  1:428625/1‑209 (MQ=255)                                                    tCTTTTTTAGCAATATATTTAAACGGCAATAATAAATAATACAAAAAACGATAATGGCATACGGAAGACATCATATCCTGCCACTCTTGAGGTTCACAATCCTGTTCATAACCGCGCATAACCGTGCGGACAGTTTCATCGGAAATATTTTCCTCACGACATAAATTATAAATAAACAAAATCAAATCGCGCACTTTCTGACGAGGTAAGTTCCGTTTTGTTGCGTCCACTTCAAAGTCAAT‑AAATAacac                                                                                                                                                                                       <  1:401883/251‑1 (MQ=255)                                                    tCTTTTTTAGCAATATATTTAAACGGCAATAATAAATAATACAAAAAACGATAATGGCATACGGAAGACATCATATCCTGCCACTCTTGAGGTTCACAATCCTGTTCATAACCGCGCATAACCGTGCGGACAGTTTCATCGGAAATATTTTCCTCACGACATAAATTATAAATAAACAAAATCAAATCGCGCACTTTCTGACGAGGTAAGTTCCGTTTTGTTGCGTCCACTTCAAAGTCAAT‑AAATAacac                                                                                                                                                                                       <  1:178341/251‑1 (MQ=255)                                                    tCTTTTTTAGCAATATATTTAAACGGCAATAATAAATAATACAAAAAACGATAATGGCATACGGAAGACATCATATCCTGCCACTCTTGAGGTTCACAATCCTGTTCATAACCGCGCATAACCGTGCGGACAGTTTCATCGGAAATATTTTCCTCACGACATAAATTATAAATAAACAAAATCAAATCGCGCACTTTCTGACGAGGTAAGTTCCGTTTTGTTGCGTCCACTTCAAAGTCAAT‑AAATAacac                                                                                                                                                                                       <  1:728717/251‑1 (MQ=255)                                                     cTTTTTTAGCAATATATTTAAACGGCAATAATAAATAATACAAAAAACGATAATGGCATACGGAAGACATCATATCCTGCCACTCTTGAGGTTCACAATCCTGTTCATAACCGCGCATAACCGTGCGGACAGTTTCATCGGAAATATTTTCCTCACGACATAAATTATAAATAAACAAAATCAAATCGCGCACTTTCTGACGAGGTAAGTTCCGTTTTGTTGCGTCCACTTCAAAGTCAAT‑AAATAacac                                                                                                                                                                                       >  1:383821/1‑250 (MQ=255)                                                     atataagaaacagatatTTAAACGGCAATAATAAATAATACAAAAAACGATAATGGCATACGGAAGACATCATATCCTGCCACTCTTGAGGTTCACAATCCTGTTCATAACCGCGCATAACCGTGCGGACAGTTTCATCGGAAATATTTTCCTCACGACATAAATTATAAATAAACAAAATCAAATCGCGCACTTTCTGACGAGGTAAGTTCCGTTTTGTTGCGTCCACTTCAAAGTCAAT‑AAATAacac                                                                                                                                                                                       <  1:160764/237‑1 (MQ=255)                                                                  atatTTAAACGGCAATAATAAATAATACAAAAAACGATAATGGCATACGGAAGACATCATATCCTGCCACTCTTGAGGTTCACAATCCTGTTCATAACCGCGCATAACCGTGCGGACAGTTTCATCGGAAATATTTTCCTCACGACATAAATTATAAATAAACAAAATCAAATCGCGCACTTTCTGACGAGGTAAGTTCCGTTTTGTTGCGTCCACTTCAAAGTCAAT‑AAATAACACCCTGCCGTCATGc                                                                                                                                                                          >  1:659209/1‑250 (MQ=255)                                                                                ataataaataataCAAAAAACGATAATGGCATACGGAAGACATCATATCCTGCCACTCTTGAGGTTCACAATCCTGTTCATAACCGCGCATAACCGTGCGGACAGTTTCATCGGAAATATTTTCCTCACGACATAAATTATAAATAAACAAAATCAAATCGCGCAc                                                                                                                                                                                                                                                 >  1:61357/1‑166 (MQ=255)                                                                                ataataaataataCAAAAAACGATAATGGCATACGGAAGACATCATATCCTGCCACTCTTGAGGTTCACAATCCTGTTCATAACCGCGCATAACCGTGCGGACAGTTTCATCGGAAATATTTTCCTCACGACATAAATTATAAATAAACAAAATCAAATCGCGCACTTTCTGAc                                                                                                                                                                                                                                         >  1:751675/1‑174 (MQ=255)                                                                                ataataaataataCAAAAAACGATAATGGCATACGGAAGACATCATATCCTGCCACTCTTGAGGTTCACAATCCTGTTCATAACCGCGCATAACCGTGCGGACAGTTTCATCGGAAATATTTTCCTCACGACATAAATTATAAATAAACAAAATCAAATCGCGCACTTTCTGACGAGGTAAGTTCCGTTTTGTTGCGTCCACTTCAAAGTCAAT‑AAATAacac                                                                                                                                                                                       >  1:73310/1‑223 (MQ=255)                                                                                ataataaataataCAAAAAACGATAATGGCATACGGAAGACATCATATCCTGCCACTCTTGAGGTTCACAATCCTGTTCATAACCGCGCATAACCGTGCGGACAGTTTCATCGGAAATATTTTCCTCACGACATAAATTATAAATAAACAAAATCAAATCGCGCACTTTCTGACGAGGTAAGTTCCGTTTTGTTGCGTCCACTTCAAAGTCAAT‑AAATAACACCCTGCCGTCATGCCATAAAATATCCCGa                                                                                                                                                           >  1:80521/1‑251 (MQ=255)                                                                                ataataaataataCAAAAAACGATAATGGCATACGGAAGACATCATATCCTGCCACTCTTGAGGTTCACAATCCTGTTCATAACCGCGCATAACCGTGCGGACAGTTTCATCGGAAATATTTTCCTCACGACATAAATTATAAATAAACAAAATCAAATCGCGCACTTTCTGACGAGGTAAGTTCCGTTTTGTTGCGTCCACTTCAAAGTCAAT‑AAATAACACCCTGCCGTCATGCCATAAAATATCCCGa                                                                                                                                                           >  1:459253/1‑251 (MQ=255)                                                                                ataataaataataCAAAAAACGATAATGGCATACGGAAGACATCATATCCTGCCACTCTTGAGGTTCACAATCCTGTTCATAACCGCGCATAACCGTGCGGACAGTTTCATCGGAAATATTTTCCTCACGACATAAATTATAAATAAACAAAATCAAATCGCGCACTTTCTGACGAGGTAAGTTCCGTTTTGTTGCGTCCACTTCAAAGTCAAT‑AAATAACACCCTGCCGTCATGCCATAAAATATCCCGa                                                                                                                                                           >  1:759097/1‑251 (MQ=255)                                                                                  aataaataataCAAAAAACGATAATGGCATACGAAAGACATCATATCCTGCCACTCTTGAGGTTCACAATCCTGTTCATAACCGCGCATAACCGTGCGGACAGTTTCATCGGAAATATTTTCCTCACGACATAAATTATAAATAAACAAAATCAAATCGCGCACTTTCTGACGAGGTAAGTTCCGTTTTGTTGCGTCCACTTCAAAGTCAAT‑AAATAACACCCTGCCGTCATGCCATAAAATATCCCGAAt                                                                                                                                                         <  1:319473/251‑1 (MQ=255)                                                                                     aaataataCAAAAAACGATAATGGCATACGGAAGACATCATATCCTGCCACTCTTGAGGTTCACAATCCTGTTCATAACCGCGCATAACCGTGCGGACAGTTTCATCGGAAATATTTTCCTCACGACATAAATTATAAATAAACAAAATCAAATCGCGCACTTTCTGACGAGGTAAGTTCCGTTTTGTTGCGTCCACTTCAAAGTCAAT‑AAATAac                                                                                                                                                                                         >  1:801963/1‑216 (MQ=255)                                                                                           taCAAAAAACGATAATGGCATACGGAAGACATCATATCCTGCCACTCTTGAGGTTCACAATCCTGTTCATAACCGCGCATAACCGTGCGGACAGTTTCATCGGAAATATTTTCCTCACGACATAAATTATAAATAAACAAAATCAAATCGCGCACTTTCTGACGAGGTAAGTTCCGTTTTGTTGCGTCCACTTCAAAGTCaat‑aaaaa                                                                                                                                                                                           >  1:829842/1‑206 (MQ=255)                                                                                                    cGATAATGGCATACGGAAGACATCATATCCTGCCACTCTTGAGGTTCACAATCCTGTTCATAACCGCGCATAACCGTGCGGACAGTTTCATCGGAAATATTTTCCTCACGACATAAATTATAAATAAACAAAATCAAATCGCGCACTTTCTGACGAGGTAAGTTCCGTTTTGTTGCGTCCACTTCAAAGTCAAT‑AAATAacac                                                                                                                                                                                       >  1:494060/1‑203 (MQ=255)                                                                                                    cGATAATGGCATACGGAAGACATCATATCCTGCCACTCTTGAGGTTCACAATCCTGTTCATAACCGCGCATAACCGTGCGGACAGTTTCATCGGAAATATTTTCCTCACGACATAAATTATAAATAAACAAAATCAAATCGCGCACTTTCTGACGAGGTAAGTTCCGTTTTGTTGCGTCCACTTCAAAGTCAAT‑AAATAACACCCTGCCGTCATGCCATAAAATATcc                                                                                                                                                              >  1:403615/1‑228 (MQ=255)                                                                                                    cGATAATGGCATACGGAAGACATCATATCCTGCCACTCTTGAGGTTCACAATCCTGTTCATAACCGCGCATAACCGTGCGGACAGTTTCATCGGAAATATTTTCCTCACGACATAAATTATAAATAAACAAAATCAAATCGCGCACTTTCTGACGAGGTAAGTTCCGTTTTGTTGCGTCCACTTCAAAGTCAAT‑AAATAACACCCTGCCGTCATGCCATAAAATATCCCGAATCGCCGGGCGCCCATGCAc                                                                                                                                       <  1:304712/251‑1 (MQ=255)                                                                                                           ggCATACGGAAGACATCATATCCTGCCACTCTTGAGGTTCACAATCCTGTTCATAACCGCGCATAACCGTGCGGACAGTTTCATCGGAAATATTTTCCTCACGACATAAATTATAAATAAACAAAATCAAATCGCGCACTTTCTGAc                                                                                                                                                                                                                                         <  1:816188/147‑1 (MQ=255)                                                                                                            gCATACGGAAGACATCATATCCTGCCACTCTTGAGGTTCACAATCCTGTTCATAACCGCGCATAACCGTGCGGACAGTTTCATCGGAAATATTTTCCTCACGACATAAATTATAAATAAACAAAATCAAATCGCGCACTTTCTGACGAGGTAAGTTCCGTTTTGTTGCGTCCACTTCAAAGTCAAT‑AAATAACACCCTGCCGTCATGCCATAAAATATCCCGAATCGCCGGGCGCCCATGCAc                                                                                                                                       >  1:161999/1‑243 (MQ=255)                                                                                                            gCATACGGAAGACATCATATCCTGCCACTCTTGAGGTTCACAATCCTGTTCATAACCGCGCATAACCGTGCGGACAGTTTCATCGGAAATATTTTCCTCACGACATAAATTATAAATAAACAAAATCAAATCGCGCACTTTCTGACGAGGTAAGTTCCGTTTTGTTGCGTCCACTTCAAAGTCAAT‑AAATAACACCCTGCCGTCATGCCATAAAATATCCCGAATCGCCGGGCGCCCATGCACCAATcc                                                                                                                                 >  1:877474/1‑249 (MQ=255)                                                                                                                aCGGAAGACATCATTTCCTGCCACTCTTGAGGTTCACAATCCTGTTCATAACCGCGCATAACCGTGCGGACAGTTTCATCGGAAATATTTTCCTCACGACATAAATTATAAATAAGCAAAATCAAATCGCGCACTTTCTGACGAGGTAAGTTCCGTTTTGTTGCGTCCACTTCAAAGTCAAT‑AAATAACACCCTGCCGTCATGCCATAAAATATCCCGAATTGCCGGGCGCCCGTGCACCAGTCCCTGTTg                                                                                                                           <  1:91730/251‑1 (MQ=255)                                                                                                                aCGGAAGACATCATATCCTGCCACTCTTGAGGTTCACAATCCTGTTCATAACCGCGCATAACCGTGCGGACAGTTTCATCGGAAATATTTTCCTCACGACATAAATTATAAATAAACAAAATCAAATCGCGCACTTTCTGACGAGGTAAGTTCCGTTTTGTTGCGTCCACTTCAAAGTCAAT‑AAATAacac                                                                                                                                                                                       <  1:859746/191‑1 (MQ=255)                                                                                                                        catcatATCCTGCCACTCTTGAGGTTCACAATCCTGTTCATAACCGCGCATAACCGTGCGGACAGTTTCATCGGAAATATTTTCCTCACGACATAAATTATAAATAAACAAAATCAAATCGCGCACTTTCTGACGAGGTAAGTTCCGTTTTGTTGCGTCCACTTCAAAGTCAAT‑AAATAACACCCTGCCGTCATGCCATAAAATATCCCGAATCGCCGGGCGCCCATGCACCAATcc                                                                                                                                 >  1:317557/1‑237 (MQ=255)                                                                                                                        catcatATCCTGCCACTCTTGAGGTTCACAATCCTGTTCATAACCGCGCATAACCGTGCGGACAGTTTCATCGGAAATATTTTCCTCACGACATAAATTATAAATAAACAAAATCAAATCGCGCACTTTCTGACGAGGTAAGTTCCGTTTTGTTGCGTCCACTTCAAAGTCAAT‑AAATAACACCCTGCCGTCATGCCATAAAATATCCCGAATCGCCGGGCGCCCATGCACCAATCCCTGTTGATGTaa                                                                                                                     >  1:287538/1‑249 (MQ=255)                                                                                                                        catcatATCCTGCCACTCTTGAGGTTCACAATCCTGTTCATAACCGCGCATAACCGTGCGGACAGTTTCATCGGAAATATTTTCCTCACGACATAAATTATAAATAAACAAAATCAAATCGCGCACTTTCTGACGAGGTAAGTTCCGTTTTGTTGCGTCCACTTCAAAGTCAAT‑AAATAACACCCTGCCGTCATGCCATAAAATATCCCGAATCGCCGGGCGCCCATGCACCAATCCCTGTTGATGTaa                                                                                                                     >  1:240711/1‑249 (MQ=255)                                                                                                                             tatCCTGCCACTCTTGAGGTTCACAATCCTGTTCATAACCGCGCATAACCGTGCGGACAGTTTCATCGGAAATATTTTCCTCACGACATAAATTATAAATAAACAAAATCAAATCGCGCACTTTCTGACGAGGTAAGTTCCGTTTTGTTGCGTCCACTTCAAAGTCAAT‑AAATAACACCCTGCCGTCATGCCATAAAATATCCCGAATCGCCGGGCGCCCATGCACCAATccc                                                                                                                                <  1:352301/233‑1 (MQ=255)                                                                                                                                       ctctTGAGGTTCACAATCCTGTTCATAACCGCGCATAACCGTGCGGACAGTTTCATCGGAAATATTTTCCTCACGACATAAATTATAAATAAACAAAATCAAATCGCGCACTTTCTGACGAGGTAAGTTCCGTTTTGTTGCGTCCACTTCAAAGTCAAT‑AAATAACACCCTGCCGTCATGCCATAAAATATCCCGAATCGCCGGGCGCCCATGCACCAATCCCTGTTGATGTAACTGCGCCAAGGCTTTAg                                                                                                    >  1:468814/1‑251 (MQ=255)                                                                                                                                        tctTGAGGTTCACAATCCTGTTCATAACCGCGCATAACCGTGCGGACAGTTTCATCGGAAATATTTTCCTCACGACATAAATTATAAATAAACAAAATCAAATCGCGCACTTTCTGACGAGGTAAGTTCCGTTTTGTTGCGTCCACTTCAAAGTCAAT‑AAATAACACCCTGCCGTCATGCCATAAAATATCCCGAATCGCCGGGCGCCCATGCACCAATCCCTGTTGATGTAACTGCGCCAAGGCTTTAGc                                                                                                   <  1:481340/251‑1 (MQ=255)                                                                                                                                          ttGAGGTTCACAATCCTGTTCATAACCGCGCATAACCGTGCGGACAGTTTCATCGGAAATATTTTCCTCACGACATAAATTATAAATAAACAAAATCAAATCGCGCACTTTCTGACGAGGTAAGTTCCGTTTTGTTGCGTCCACTTCAAAGTCAAT‑AAATAACACCCTGCCGTCATGCCATAAAATATCCCGAATCGCCGGGCGCCCATGCACCAATCCCTGTTGATGTAACTGCGCCAAGGCTTTAGcac                                                                                                 <  1:394469/251‑1 (MQ=255)                                                                                                                                                 tCACAATCCTGTTCATAACCGCGCATAACCGTGCGGACAGTTTCATCGGAAATATTTTCCTCACGACATAAATTATAAATAAACAAAATCAAATCGCGCACTTTCTGACGAGGTAAGTTCCGTTTTGTTGCGTCCACTTCAAAGTCAAT‑AAATAACACCCTGCCGTCATGCCATAAAATATCCCGAATCGCCGGGCGCCCATGCACCAAt                                                                                                                                   <  1:692419/210‑1 (MQ=255)                                                                                                                                                    caATCCTGTTCATAACCGCGCATAACCGTGCGGACAGTTTCATCGGAAATATTTTCCTCACGACATAAATTATAAATAAACAAAATCAAATCGCGCACTTTCTGACGAGGTAAGTTCCGTTTTGTTGCGTCCACTTCAAAGTCAAT‑AAATAACACCCTGCCGTCATGCCATAAAATATCCCGAATCGCCGGGCGCCCATGCACCAATCCCTGTTGATGTAACTGCGCCAAGGCTTTAGCACAATCcagcag                                                                                       <  1:428189/251‑1 (MQ=255)                                                                                                                                                    caATCCTGTTCATAACCGCGCATAACCGTGCGGACAGTTTCATCGGAAATATTTTCCTCACGACATAAATTATAAATAAACAAAATCAAATCGCGCACTTTCTGACGAGGTAAGTTCCGTTTTGTTGCGTCCACTTCAAAGTCAAT‑AAATAACACCCTGCCGTCATGCCATAAAATATCCCGAATCGCCGGGCGCCCATGCACCAATCCCTGTTGATGTAACTGCGCCAAGGCTTTAGCACAATCcagcag                                                                                       <  1:179350/251‑1 (MQ=255)                                                                                                                                                      aTCCTGTTCATAACCGCGCATAACCGTGCGGACAGTTTCATCGGAAATATTTTCCTCACGACATAAATTATAAATAAACAAAATCAAATCGCGCACTTTCTGACGAGGTAAGTTCCGTTTTGTTGCGTCCACTTCAAAGTCaat‑aa                                                                                                                                                                                              <  1:639661/146‑1 (MQ=255)                                                                                                                                                      aTCCTGTTCATAACCGCGCATAACCGTGCGGACAGTTTCATCGGAAATATTTTCCTCACGACATAAATTATAAATAAACAAAATCAAATCGCGCACTTTCTGACGAGGTAAGTTCCGTTTTGTTGCGTCCACTTCAAAGTCAAT‑AAATAACACCCTGCCGTCATGCCATAAAATATCCCGAATCGCCGGGCGCCCATGCACTAATCCCTGTTGATGTAACTGCGCCAAGGCTTTAGCACAATCCAGCAGaa                                                                                     >  1:77352/1‑251 (MQ=255)                                                                                                                                                      aTCCTGTTCATAACCGCGCATAACCGTGCGGACAGTTTCATCGGAAATATTTTCCTCACGACATAAATTATAAATAAACAAAATCAAATCGCGCACTTTCTGACGAGGTAAGTTCCGTTTTGTTGCGTCCACTTCAAAGTCAAT‑AAATAACACCCTGCCGTCATGCCATAAAATATCCCGAATCGCCGGGCGCCCATGCACCAATCCCTGTTGATGTAACTGCGCCAAGGCTTTAGcac                                                                                                 <  1:589444/239‑1 (MQ=255)                                                                                                                                                      aTCCTGTTCATAACCGCGCATAACCGTGCGGACAGTTTCATCGGAAATATTTTCCTCACGACATAAATTATAAATAAACAAAATCAAATCGCGCACTTTCTGACGAGGTAAGTTCCGTTTTGTTGCGTCCACTTCAAAGTCAAT‑AAATAACACCCTGCCGTCATGCCATAAAATATCCCGAATCGCCGGGCGCCCATGCACCAATCCCTGTTGATGTAACTGCGCCAAGGCTTTAGCACAATCCAGCAGaa                                                                                     >  1:538212/1‑251 (MQ=255)                                                                                                                                                           gTTCATAACCGCGCATAACCGTGCGGACAGTTTCATCGGAAATATTTTCCTCATGACATAAATTATAAATAAACAAAATCAAATCGCGCACTTTCTGACGAGGTAAGTTCCGTTTTGTTGCGTCCACTTCAAAGTCAAT‑AAATAACACCCTGCCGTCATGCCATAAAATATCCCGAATCGCCGGGCGCCCATGCAc                                                                                                                                       >  1:121512/1‑196 (MQ=255)                                                                                                                                                           gTTCATAACCGCGCATAACCGTGCGGACAGTTTCATCGGAAATATTTTCCTCACGACATAAATTATAAATAAACAAAATCAAATCGCGCACTTTCTGACGAGGTAAGTTCCGTTTTGTTGCGTCCACTTCAAAGTCAAT‑AAATAACACCCTGCCGTCATGCCATAAAATATCCCGAATCGCCGGGCGCCCATGCACCAATCCCTGTTGATGTAACTGCGCCAAGGCTTTAGCACAATCCAGCAGAAt                                                                                    >  1:549284/1‑247 (MQ=255)                                                                                                                                                           gTTCATAACCGCGCATAACCGTGCGGACAGTTTCATCGGAAATATTTTCCTCACGACATAAATTATAAATAAACAAAATCAAATCGCGCACTTTCTGACGAGGTAAGTTCCGTTTTGTTGCGTCCACTTCAAAGTCAAT‑AAATAACACCCTGCCGTCATGCCATAAAATATCCCGAATCGCCGGGCGCCCATGCACCAATCCCTGTTGATGTAACTGCGCCAAGGCTTTAGCACAATCCAGCAGAAt                                                                                    >  1:837116/1‑247 (MQ=255)                                                                                                                                                                     gcgcATAACCGTGCGGACAGTTTCATCGGAAATATTTTCCTCACGACATAAATTATAAATAAACAAAATCAAATCGCGCACTTTCTGACGAGGTAAGTTCCGTTTTGTTGCGTCCACTTCAAAGt                                                                                                                                                                                                     <  1:91707/125‑1 (MQ=255)                                                                                                                                                                     gcgcATAACCGTGCGGACAGTTTCATCGGAAATATTTTCCTCACGACATAAATTATAAATAAACAAAATCAAATCGCGCACTTTCTGACGAGGTAAGTTCCGTTTTGTTGCGTCCACTTCAAAGTc                                                                                                                                                                                                    >  1:422116/1‑126 (MQ=255)                                                                                                                                                                       gcATAACCGTGCGGACAGTTTCATCGGAAATATTTTCCTCACGACATAAATTATAAATAAACAAAATCAAATCGCGCACTTTCTGACGAGGTAAGTTCCGTTTTGTTGCGTCCACTTCAAAGTCAAT‑AAATAACACCCTGCCGTCATGCCATAAAATATCCCGAATCGCCGGGCGCCCATGCAc                                                                                                                                       <  1:145456/184‑1 (MQ=255)                                                                                                                                                                        cATAACCGTGCGGACAGTTTCATCGGAAATATTTTCCTCACGACATAAATTATAAATAAACAAAATCAAATCGCGCACTTTCTGACGAGGTAAGTTCCGTTTTGTTGCGTCCACTTCAAAGTCAAT‑AAATAACACCCTGCCGTCATGCCATAAAATATCCCGAATCGCCGGGCGCCCATGCACCAATCCCTGTTGATGTAACTGCGCCAAGGCTTTAGCACAATCcagcag                                                                                       <  1:357094/231‑1 (MQ=255)                                                                                                                                                                          tAACCGTGCGGACAGTTTCATCGGAAATATTTTCCTCACGACATAAATTATAAATAAACAAAATCAAATCGCGCACTTTCTGACGAGGTAAGTTCCGTTTTGTTGCGTCCACTTCAAAGTCAAT‑AAATAACACCCTGCCGTCATGCCATAAAATATCCCGAATCGCCGGGCGCCCATGCACCAATCCCTGTTGATGTAACTGCGCCAAGGCTTTAGCACAATCCAGCAGAATCTGTTGTTTGGTTTGCGtt                                                                 >  1:660425/1‑251 (MQ=255)                                                                                                                                                                          tAACCGTGCGGACAGTTTCATCGGAAATATTTTCCTCACGACATAAATTATAAATAAACAAAATCAAATCGCGCACTTTCTGACGAGGTAAGTTCCGTTTTGTTGCGTCCACTTCAAAGTCAAT‑AAATAACACCCTGCCGTCATGCCATAAAATATCCCGAATCGCCGGGCGCCCATGCACCAATCCCTGTTGATGTAACTGCGCCAAGGCTTTAGCACAATCCAGCAGAATCTGTTGTTTGGTTTGCGtt                                                                 >  1:318272/1‑251 (MQ=255)                                                                                                                                                                              cGTGCGGACAGTTTCATCGGAAATATTTTCCTCACGACATAAATTATAAATAAACAAAATCAAATCGCGCACTTTCTGACGAGGTAAGTTCCGTTTTGTTGCGTCCACTTCAAAGTCAATAAAATAACACCCTGCCGTCATGCCATAAAATATCCCGAATCGCCGGGCGCCCATGCACCAATCCCTGTTGATGTAACTGCGCCAAGGCTTTAGCACAATCCAGCAGAATCTGTTGTTTGGTTTGCGTTggg                                                              >  1:337128/1‑251 (MQ=255)                                                                                                                                                                              cGTGCGGACAGTTTCATCGGAAATATTTTCCTCACGACATAAATTATAAATAAACAAAATCAAATCGCGCACTTTCTGACGAGGTAAGTTCCGTTTTGTTGCGTCCACTTCAAAGTCAAT‑AAATAACACCCTGCCGTCATGCCATAAAATATCCCGAATCGCCGGGCGCCCATGCACCAATCCCTGTTGATGTAACTGCGCCAAGGCTTTAGCACAATCcagcag                                                                                       >  1:650451/1‑225 (MQ=255)                                                                                                                                                                               gTGCGGACAGTTTCATCGGAAATATTTTCCTCACGACATAAATTATAAATAAACAAAATCAAATCGCGCACTTTCTGACGAGGTAAGTTCCGTTTTGTTGCGTCCACTTCAAAGTCAAT‑AAATAACACCCTGCCGTCATGCCATAAAATATCCCGAATCGCCGGGCGCCCATGCACCAATCCCTGTTGATGTAACTGCGCCAAGGCTTTAGCACAATCCAGCAGAATCTGTTGTTTGGTTTGCGTTGGGat                                                            >  1:176368/1‑251 (MQ=255)                                                                                                                                                                                tGCGGACAGTTTCATCGGAAATATTTTCCTCACGACATAAATTATAAATAAACAAAATCAAATCGCGCACTTTCTGACGAGGTAAGTTCCGTTTTGTTGCGTCCACTTCAAAGTCAAT‑AAATAACACCCTGCCGTCATGCCATAAAATATCCCGAATCGCCGGGCGCCCATGCACCAATCCCTGTTGATGTAACTGCGCCAAGGCTTTAGCACAATCCAGCAGAATCTGTTGTTTGGTTTGCGTTGGGat                                                            <  1:76227/250‑1 (MQ=255)                                                                                                                                                                                    gACAGTTTCATCGGAAATATTTTCCTCACGACATAAATTATAAATAAACAAAATCAAATCGCGCACTTTCTGACGAGGTAAGTTCCGTTTTGTTGCGTCCACTTCAAAGTCAAT‑AAATAACACCCTGCCGTCATGCCATAAAATATCCCGAATCGCCGGGCGCCCATGCACCAATCCCTGTTGATGTAACTGCGCCAAGGCTTTAGCACAATCCAGCAGAATCTGTTGTTTGGTTTGCGTTGGGAtattat                                                       >  1:675054/1‑251 (MQ=255)                                                                                                                                                                                        gTTTCATCGGAAATATTTTCCTCACGACATAAATTATAAATAAACAAAATCAAATCGCGCACTTTCTGACGAGGTAAGTTCCGTTTTGTTGCGTCCACTTCAAAGTCAAT‑AAATAACACCCTGCCGTCATGCCATAAAATATCCCGAATCGCCGGGCGCCCATGCACCAATCCCTGTTGATGTAACTGCGCCAAGGCTTTAGcac                                                                                                 >  1:110120/1‑205 (MQ=255)                                                                                                                                                                                         tttCATCGGAAATATTTTCCTCACGACATAAATTATAAATAAACAAAATCAAATCGCGCACTTTCTGACGAGGTAAGTTCCGTTTTGTTGCGTCCACTTCAAAGTCAAT‑AAATAACACCCTGCCGTCATGCCATAAAATATCCCGAATCGCCGGGCGCCCATGCACCAATCCCTGTTGATGTAACTGCGCCAAGGCTTTAGCACAATCCAGCAGAATCTGTTGTTTGGTTTGCGTTGGGAtattat                                                       <  1:300802/246‑1 (MQ=255)                                                                                                                                                                                         tttCATCGGAAATATTTTCCTCACGACATAAATTATAAATAAACAAAATCAAATCGCGCACTTTCTGACGAGGTAAGGTCCGTTTTGTTGCGTCCACTTCAAAGTCAAT‑AAATAACACCCTGCCGTCATGCCATAAAATATCCCGAATCGCCGGGCGCCCATGCACCAATCCCTGTTGATGTAACTGCGCCAAGGCTTTAGCACAATCCAGCAGAATCTGTTGTTTGGTTTGCGTTGGGATATTATc                                                      <  1:429144/247‑1 (MQ=255)                                                                                                                                                                                                   aaTATTTTCCTCACGACATAAATTATAAATAAACAAAATCAAATCGCGCACTTTCTGACGAGGTAAGTTCCGTTTTGTTGCGTCCACTTCAAAGTCAAT‑AAATAACACCCTGCCGTCATGCCATAAAATATCCCGAATCGCCGGGCGCCCATGCACCAATCCCTGTTGATGTAACTGCGCCAAGGCTTTAGCACAATCCAGCAGAATCTGTTGTTTGGTTTGCGTTGGGATATTATCg                                                     >  1:323369/1‑238 (MQ=255)                                                                                                                                                                                                                  aCATAAATTATAAATAAACAAAATCAAATCGCGCACTTTCTGACGAGGTAAGTTCCGTTTTGTTGCGTCCACTTCAAAGTCAAT‑AAATAACACCCTGCCGTCATGCCATAAAATATCCCGAATCGCCGGGCGCCCATGCAc                                                                                                                                       <  1:738361/141‑1 (MQ=255)                                                                                                                                                                                                                  aCATAAATTATAAATAAACAAAATCAAATCGCGCACTTTCTGACGAGGTAAGTTCCGTTTTGTTGCGTCCACATCAAAGTCAAT‑AAATAACACCCTGCCGTCATGCCATAAAATATCCCGAATCGCCGGGCGCCCATGCACCAATCCCTGTTGATGTAACTGCGCCAAGGCTTTAGCACAATCCAGCAGAATCTGTTGTTTGGTTTGCGTTGGGATATTATCGTCAACCCAATTTGCTACGCTTTTGcca                          <  1:318426/250‑1 (MQ=255)                                                                                                                                                                                                                   cATAAATTATAAATAAACAAAATCAAATCGCGCACTTTCTGACGAGGTAAGTTCCGTTTTGTTGCGTCCACTTCAAAGTCAAT‑AAATAACACCCTGCCGTCATGCCATAAAATATCCCGAATCGCCGGGCGCCCATGCACCAATCCCTGTTGATGTAACTGCGCCAAGGCTTTAGCACAATCcagcag                                                                                       <  1:403675/188‑1 (MQ=255)                                                                                                                                                                                                                       aaTTATAAATAAACAAAATCAAATCGCGCACTTTCTGACGAGGTAAGTTCCGTTTTGTTGCGTCCACTTCAAAGTCAAT‑AAATAACACCCTGCCGTCATGCCATAAAATATCCCGAATCGCCGGGCGCCCATGCACCAATCCCTGTTGATGTAACTGCGCCAAGGCTTTAGCACAATCCAGCAGAATCTGTTGTTTGGTTTGCGTTGGGATATTATCg                                                     >  1:885305/1‑218 (MQ=255)                                                                                                                                                                                                                       aaTTATAAATAAACAAAATCAAATCGCGCACTTTCTGACGAGGTAAGTTCCGTTTTGTTGCGTCCACTTCAAAGTCAAT‑AAATAACACCCTGCCGTCATGCCATAAAATATCCCGAATCGCCGGGCGCCCATGCACCAATCCCTGTTGATGTAACTGCGCCAAGGCTTTAGCACAATCCAGCAGAATCTGTTGTTTGGTTTGCGTTGGGATATTATCGTCAACCCAATTTGCTACGCTTTTGCCACCAt                      >  1:886664/1‑249 (MQ=255)                                                                                                                                                                                                                        aTTATAAATAAACAAAATCAAATCGCGCACTTTCTGACGAGGTAAGTTCCGTTTTGTTGCGTCCACTTCAAAGTCAAT‑AAATAac                                                                                                                                                                                         <  1:115742/85‑1 (MQ=255)                                                                                                                                                                                                                        aTTATAAATAAACAAAATCAAATCGCGCACTTTCTGACGAGGTAAGTTCCGTTTTGTTGCGTCCACTTCAAAGTCAAT‑AAATAACACCCTGCCGTCATGCCATAAAATATCCCGAATCGCCGGGCGCCCATGCACCAATCCCTGTTGATGTAACTGCGCCAAGGCTTTAGCACAATCCAGCAGAATCTGTTGTTTGGTTTGCGTTGGGATATTATCGTCAACCCAATTTg                                        >  1:865993/1‑230 (MQ=255)                                                                                                                                                                                                                        aTTATAAATAAACAAAATCAAATCGCGCACTTTCTGACGAGGTAAGTTCCGTTTTGTTGCGTCCACTTCAAAGTCAAT‑AAATAACACCCTGCCGTCATGCCATAAAATATCCCGAATCGCCGGGCGCCCATGCACCAATCCCTGTTGATGTAACTGCGCCAAGGCTTTAGCACAATCCAGCAGAATCTGTTGTTTGGTTTGCGTTGGGATATTATCGTCAACCCAATTTGCTACGCTTTTGCCACCATCtt                   >  1:569673/1‑251 (MQ=255)                                                                                                                                                                                                                            taaataaaCAAAATCAAATCGCGCACTTTCTGACGAGGTAAGTTCCGTTTTGTTGCGTCCACTTCAAAGTCAAT‑AAATAACACCCTGCCGTCATGCCATAAAATATCCCGAATCGCCGGGCGCCCATGCACCAATCCCTGTTGATGTAACTg                                                                                                                  >  1:119081/1‑152 (MQ=255)                                                                                                                                                                                                                                      aaatcaaatcGCGCACTTTCTGACGAGGTAAGTTCCGTTTTGTTGCGTCCACTTCAAAGt                                                                                                                                                                                                     <  1:469829/60‑1 (MQ=255)                                                                                                                                                                                                                                      aaatcaaatcGCGCACTTTCTGACGAGGTAAGTTCCGTTTTGTTGCGTCCACTTCAAAGTCAAT‑AAATAACACCCTGCCGTCATGCCATAAAATATCCCGAATCGCCGGGCGCCCATGCACCAATcc                                                                                                                                 <  1:586764/127‑1 (MQ=255)                                                                                                                                                                                                                                      aaatcaaatcGCGCACTTTCTGACGAGGTAAGTTCCGTTTTGTTGCGTCCACTTCAAAGTCAAT‑AAATAACACCCTGCCGTCATGCCATAAAATATCCCGAATCGCCGGGCGCCCATGCACCAATCCCTGTTGATGTAACTGCGCCAAGGCTTTAGCACAATCCAGCAGAATCtgttg                                                                              <  1:71302/178‑1 (MQ=255)                                                                                                                                                                                                                                      aaatcaaatcGCGCACTTTCTGACGAGGTAAGTTCCGTTTTGTTGCGTCCACTTCAAAGTCAAT‑AAATAACACCCTGCCGTCATGCCATAAAATATCCCGAATCGCCGGGCGCCCATGCACCAATCCCTGTTGATGTAACTGCGCCAAGGCTTTAGCACAATCCAGCAGAATCTGTTGTTTGGTTTGCGTTGGGATATTATCGTCAACCCAATTTGCTACGCTTTTGCCACCATCTTCCAGCACCAGATaa     >  1:415525/1‑251 (MQ=255)                                                                                                                                                                                                                                         tcaaatcgCGCACTTTCTGACGAGGTAAGTTCCGTTTTGTTGCGTCCACTTCAAAGTCAAT‑AAATAACACCCTGCCGTCATGCCATAAAATATCCCGAATCGCCGGGCGCCCATGCACCAATCCCTGTTGATGTAACTGCGCCAAGGCTTTAGCACAATCCAGCAGAATCTGTTGTTTGGTTTGCGTTGGGATATTATCGTCAACCCAATTTGCTACGCTTTTGCCACCATCTTCCAGCACCAGATAATCt  <  1:532016/251‑1 (MQ=255)                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                     CCTCTTTTCACTTTTTCCACATTTCTAAATAAACGATACACCCCGATTAAATCTTTTTTAGCAATATATTTAAACGGCAATAATAAATAATACAAAAAACGATAATGGCATACGGAAGACATCATATCCTGCCACTCTTGAGGTTCACAATCCTGTTCATAACCGCGCATAACCGTGCGGACAGTTTCATCGGAAATATTTTCCTCACGACATAAATTATAAATAAACAAAATCAAATCACGCACTTTCTGACGAGGTAAGTTCCGTTTTGTTGCGTCCACTTCAAAGTCAAT‑AAATAACACCCTGCCGTCATGCCATAAAATATCCCGAATCGCCGGGCGCCCATGCACCAATCCCTGTTGATGTAACTGCGCCAAGGCTTTAGCACAATCCAGCAGAATCTGTTGTTTGGTTTGCGTTGGGATATTATCGTCAACCCAATTTGCTACGCTTTTGCCACCATCTTCCAGCACCAGATAATCT  >  1/1821288‑1821770

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑