Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,810,064	T→A	intergenic (+15137/‑124267)	unknown → / → unknown	tRNA‑Leu‑CAA/tRNA‑Asp‑GTC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,810,064	0	T	A	100.0%	165.3 / NA	62	intergenic (+15137/‑124267)	unknown/unknown	tRNA‑Leu‑CAA/tRNA‑Asp‑GTC
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base A (37/25);  total (37/25)

GGTTAATTAATATAAATTATAGCGCACGCGCGGTTCAGCCCAAAAGAAGCGGGACGAAAATCCCCAGCCGTAAGGGTAAAACGGTGCGTAGTCATAAATGTCGTCAGGGTCGTAATAATATTCTTTCACCAGCTTCCAAATACGGTATTGATCGGCTTGAACCACGGGATAATTGTAATCCGCCTGATCAATTTTGCCCTGCTCTTTGCCGCTCAGCACTCCGCCGACTGTCACATACTGGTCTTTCAGATTTTCCGGATCCACAAAGCCGTTTAAATAGGCGATAAAACGTCCGTTGCTATAGCTATCCACGATAGGTTTCGCGGAAAAATACGCCACCGTTTGATTCAATACTTCAATTTTGGTTTTATCTTTCAACGCCTGTGCGGAAACCACTTTGCCGCCCAAGCGCACTTTCAGGCAGCGGCAATCGTAATCCTGCGCCTGCACATTTTTCAGATTTAACATTTCGAACTGTTT  >  1/1809826‑1810305                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                                  ggTTAATTAATATAAATTATAGCGCACGCGCGGTTCCGCCCAAAAGAAGCGGGACGAAAATCCCCAGCCGTAAGGGTAAAACGGTGCGTAGTCATAAATGTCGTCAGGGTCGTAATAATATTCTTTCACCAGCTTCCAAATACGGTATTGATCGGCTTGAACCACGGGATAATTGTAATCCGCCTGATCAATTTTGCCCTGCTCTTTGCCGCTCAGCACTCCGCCGACTGTCACATACAGGTCTTTCAGAt                                                                                                                                                                                                                                       >  1:371782/1‑251 (MQ=255)            ataAATTATAGCGCACGCGCGGTTCAGCCCAAAAGAAGCGGGACGAAAATCCCCAGCCGTAAGGGTAAAACGGTGCGTAGTCATAAATGTCGTCAGGGTCGTAATAATATTCTTTCACCAGCTTCCAAATACGGTATTGATCGGCTTGAACCACGGGATAATTGTAATCCGCCTGATCAATTTTGCCCTGCTCTTTGCCGCTCAGCACTCCGCCGACTGTCACATACAGGTCTTTCAGATTTTCCGGATc                                                                                                                                                                                                                             >  1:19452/1‑250 (MQ=255)                           cgcgcgGTTCAGCCCAAAAGAAGCGGGACGAAAATCCCCAGCCGTAAGGGTAAAACGGTGCGTAGTCATAAATGTCGTCAGGGTCGTAATAATATTCTTTCACCAGCTTCCAAATACGGTATTGATCGGCTTGAACCACGGGATAATTGTAATCCGCCTGATCAATTTTGCCCTGCTCTTTGCCGCTCAGCACTCCGCCGACTGTCACATACAGGTCTTTCAGATTTTCCGGATCCACAAAGCCGTTTaaa                                                                                                                                                                                                             <  1:17181/251‑1 (MQ=255)                             cgcgGTTCAGCCCAAAAGAAGCGGGACGAAAATCCCCAGCCGTAAGGGTAAAACGGTGCGTAGTCATAAATGTCGTCAGGGTCGTAATAATATTCTTTCACCAGCTTCCAAATACGGTATTGATCGGCTTGAACCACGGGATAATTGTAATCCGCCTGATCAATTTTGCCCTGCTCTTTGCCGCTCAGCACTCCGCCGACTGTCACATACAGGTCTTTCAGATTTTCCGGATCCACAAAGCCGTTTAAAt                                                                                                                                                                                                            <  1:462922/250‑1 (MQ=255)                                 gTTCAGCCCAAAAGAAGCGGGACGAAAATCCCCAGCCGTAAGGGTAAAACGGTGCGTAGTCATAAATGTCGTCAGGGTCGTAATAATATTCTTTCACCAGCTTCCAAATACGGTATTGATCGGCTTGAACCACGGGATAATTGTAATCCGCCTGATCAATTTTGCCCTGCTCTTTGCCGCTCAGCACTCCGCCGACTGTCACATACAGGTCTTTCAGATTTTCCGGATCCACAAAGCCGTTTAAATAGGCg                                                                                                                                                                                                       >  1:354664/1‑251 (MQ=255)                                 gTTCAGCCCAAAAGAAGCGGGACGAAAATCCCCAGCCGTAAGGGTAAAACGGTGCGTAGTCATAAATGTCGTCAGGGTCGTAATAATATTCTTTCACCAGCTTCCAAATACGGTATTGATCGGCTTGAACCACGGGATAATTGTAATCCGCCTGATCAATTTTGCCCTGCTCTTTGCCGCTCAGCACTCCGCCGACTGTCACATACAGGTCTTTCAGATTTTCCGGATCCACAAAGCCGTTTAAATAGGCg                                                                                                                                                                                                       >  1:788642/1‑251 (MQ=255)                                 gTTCAGCCCAAAAGAAGCGGGACGAAAATCCCCAGCCGTAAGGGTAAAACGGTGCGTAGTCATAAATGTCGTCAGGGTCGTAATAATATTCTTTCACCAGCTTCCAAATACGGTATTGATCGGCTTGAACCACGGGATAATTGTAATCCGCCTGATCAATTTTGCCCTGCTCTTTGCCGCTCAGCACTCCGCCGACTGTCACATACAGGTCTTTCAGATTTTCCGGATCCACAAAGCCGTTTAAATAGGCg                                                                                                                                                                                                       >  1:635785/1‑251 (MQ=255)                                 gTTCAGCCCAAAAGAAGCGGGACGAAAATCCCCAGCCGTAAGGGTAAAACGGTGCGTAGTCATAAATGTCGTCAGGGTCGTAATAATATTCTTTCACCAGCTTCCAAATACGGTATTGATCGGCTTGAACCACGGGATAATTGTAATCCGCCTGATCAATTTTGCCCTGCTCTTTGCCGCTCAGCACTCCGCCGACTGTCACATACAGGTCTTTCAGATTTTCCGGATCCACAAAGCCGTTTAAATAGGCg                                                                                                                                                                                                       >  1:710125/1‑251 (MQ=255)                                       cccAAAAGAAGCGGGACGAAAATCCCCAGCCGTAAGGGTAAAACGGTGCGTAGTCATAAATGTCGTCAGGGTCGTAATAATATTCTTTCACCAGCTTCCAAATACGGTATTGATCGGCTTGAACCACGGGATAATTGTAATCCGCCTGATCAATTTTGCCCTGCTCTTTGCCGCTCAGCACTCCGCCGACTGTCACATACAGGTCTTTCAGATTTTCCGGATCCACAAAGCCGTTTAAATAGGCGATaaa                                                                                                                                                                                                  <  1:15489/250‑1 (MQ=255)                                                  cGGGACGAAAATCCCCAGCCGTAAGGGTAAAACGGTGCGTAGTCATAAATGTCGTCAGGGTCGTAATAATATTCTTTCACCAGCTTCCAAATACGGTATTGATCGGCTTGAACCACGGGATAATTGTAATCCGCCTGATCAATTTTGCCCTGCTCTTTGCCGCTCAGCACTCCGCCGACTGTCACATACAGGTCTTTCAGATTTTCCGGATCCACAAAGCCGTTTAAATAGGCGATAAAACGTCCGTTg                                                                                                                                                                                        <  1:457506/249‑1 (MQ=255)                                                      aCGAAAATCCCCAGCCGTAAGGGTAAAACGGTGCGTAGTCATAAATGTCGTCAGGGTCGTAATAATATTCTTTCACCAGCTTCCAAATACGGTATTGATCGGCTTGAACCACGGGATAATTGTAATCCGCCTGATCAATTTTGCCCTGCTCTTTGCCGCTCAGCACTCCGCCGACTGTCACATACAGGTCTTTCAGATTTTCCGGATCCACAAAGCCGTTTAAATAGGCGATAAAACGTCCGTTGCTATAg                                                                                                                                                                                  <  1:765091/251‑1 (MQ=255)                                                       cGAAAATCCCCAGCCGTAAGGGTAAAACGGTGCGTAGTCATAAATGTCGTCAGGGTCGTAATAATATTCTTTCACCAGCTTCCAAATACGGTATTGATCGGCTTGAACCACGGGATAATTGTAATCCGCCTGATCAATTTTGCCCTGCTCTTTGCCGCTCAGCACTCCGCCGACTGTCACATACAGGTCTTTCAGATTTTCCGGATCCACAAAGCCGTTTAAATAGGCGATAAAACGTCCGTTGCTATAGc                                                                                                                                                                                 <  1:745145/251‑1 (MQ=255)                                                       cGAAAATCCCCAGCCGTAAGGGTAAAACGGTGCGTAGTCATAAATGTCGTCAGGGTCGTAATAATATTCTTTCACCAGCTTCCAAATACGGTATTGATCGGCTTGAACCACGGGATAATTGTAATCCGCCTGATCAATTTTGCCCTGCTCTTTGCCGCTCAGCACTCCGCCGACTGTCACATACAGGTCTTTCAGATTTTCCGGATCCACAAAGCCGTTTAAATAGGCGATAAAACGTCCGTTGCTATAGc                                                                                                                                                                                 <  1:755557/251‑1 (MQ=255)                                                       cGAAAATCCCCAGCCGTAAGGGTAAAACGGTGCGTAGTCATAAATGTCGTCAGGGTCGTAATAATATTCTTTCACCAGCTTCCAAATACGGTATTGATCGGCTTGAACCACGGGATAATTGTAATCCGCCTGATCAATTTTGCCCTGCTCTTTGCCGCTCAGCACTCCGCCGACTGTCACATACAGGTCTTTCAGATTTTCCGGATCCACAAAGCCGTTTAAATAGGCGATAAAACGTCCGTTGCTATAGc                                                                                                                                                                                 <  1:549499/251‑1 (MQ=255)                                                       cGAAAATCCCCAGCCGTAAGGGTAAAACGGTGCGTAGTCATAAATGTCGTCAGGGTCGTAATAATATTCTTTCACCAGCTTCCAAATACGGTATTGATCGGCTTGAACCACGGGATAATTGTAATCCGCCTGATCAATTTTGCCCTGCTCTTTGCCGCTCAGCACTCCGCCGACTGTCACATACAGGTCTTTCAGATTTTCCGGATCCACAAAGCCGTTTAAATAGGCGATAAAACGTCCGTTGCTATAGc                                                                                                                                                                                 <  1:817153/251‑1 (MQ=255)                                                         aaaaTCCCCAGCCGTAAGGGTAAAACGGTGCGTAGTCATAAATGTCGTCAGGGTCGTAATAATATTCTTTCACCAGCTTCCAAATACGGTATTGATCGGCTTGAACCACGGGATAATTGTAATCCGCCTGATCAATTTTGCCCTGCTCTTTGCCGCTCAGCACTCCGCCGACTGTCACATACAGGTCTTTCAGATTTTCCGGATCCACAAAGCCGTTTAAATAGGCGATAAAACGTCCGTTGCTATAGCt                                                                                                                                                                                <  1:419038/250‑1 (MQ=255)                                                            aTCCCCAGCCGTAAGGGTAAAACGGTGCGTAGTCATAAATGTCGTCAGGGTCGTAATAATATTCTTTCACCAGCTTCCAAATACGGTATTGATCGGCTTGAACCACGGGATAATTGTAATCCGCCTGATCAATTTTGCCCTGCTCTTTGCCGCTCAGCACTCCGCCGACTGTCACATACAGGTCTTTCAGATTTTCCGGATCCACAAAGCCGTTTAAAt                                                                                                                                                                                                            >  1:231079/1‑219 (MQ=255)                                                              ccccAGCCGTGAGGGTAAAACGGTGCGTAGTCATAAATGTCGTCAGGGTCGTAATAATATTCTTTCACCAGCTTCCAAATACGGTATTGATCGGCTTGAACCACGGGATAATTGTAATCCGCCTGATCAATTTTGCCCTGCTCTTTGCCGCTCAGCACTCCGCCGACTGTCACATACAGGTCTTTCAGATTTTCCGGATCCACAAAGCCGTTTAAATAGGCGATAAAACGTCCGTTGCTATAGc                                                                                                                                                                                 <  1:878040/244‑1 (MQ=255)                                                                          gggTAAAACGGTGCGTAGTCATAAATGTCGTCAGGGTCGTAATAATATTCTTTCACCAGCTTCCAAATACGGTATTGATCGGCTTGAACCACGGGATAATTGTAATCCGCCTGATCAATTTTGCCCTGCTCTTTGCCGCTCAGCACTCCGCCGACTGTCACATACAGGTCTTTCAGATTTTCCGGATCCACAAAGCCGTTTAAATAGGCGATAAAACGTCCGTTGCTATAGCTATCCACGAt                                                                                                                                                                       >  1:61572/1‑242 (MQ=255)                                                                           ggTAAAACGGTGCGTAGTCATAAATGTCGTCAGGGTCGTAATAATATTCTTTCACCAGCTTCCAAATACGGTATTGATCGGCTTGAACCACGGGATAATTGTAATCCGCCTGATCAATTTTGCCCTGCTCTTTGCCGCTCAGCACTCCGCCGACTGTCACATACAGGTCTTTCAGATTTTCCGGATCCACAAAGCCGTTTAAATAGGCGATAAAACGTCCGTTGCTATAGc                                                                                                                                                                                 >  1:239530/1‑231 (MQ=255)                                                                                     tGCGTAGTCATAAATGTCGTCAGGGTCGTAATAATATTCTTTCACCAGCTTCCAAATACGGTATTGATCGGCTTGAACCACGGGATAATTGTAATCCGCCTGATCAATTTTGCCCTGCTCTTTGCCGCTCAGCACTCCGCCGACTGTCACATACAGGTCTTTCAGATTTTCCGGATCCACAAAGCCGTTTAAATAGGCGATAAAACGTCCGTTGCTATAGCTATCCACGATAGGTTTCGCGGAAAAATAc                                                                                                                                                    <  1:31341/250‑1 (MQ=255)                                                                                           gTCATAAATGTCGTCAGGGTCGTAATAATATTCTTTCACCAGCTTCCAAATACGGTATTGATCGGCTTGAACCACGGGATAATTGTAATCCGCCTGATCAATTTTGCCCTGCTCTTTGCCGCTCAGCACTCCGCCGACTGTCACATACAGGTCTTTCAGATTTTCCGGATCCACAAAGCCGTTTAAATAGGCGATAAAACGTCCGTTGCTATAg                                                                                                                                                                                  <  1:800376/214‑1 (MQ=255)                                                                                           gTCATAAATGTCGTCAGGGTCGTAATAATATTCTTTCACCAGCTTCCAAATACGGTATTGATCGGCTTGAACCACGGGATAATTGTAATCCGCCTGATCAATTTTGCCCTGCTCTTTGCCGCTCAGCACTCCGCCGACTGTCACATACAGGTCTTTCAGATTTTCCGGATCCACAAAGCCGTTTAAATAGGCGATAAAACGTCCGTTGCTATAGCTATCCAc                                                                                                                                                                          >  1:368704/1‑222 (MQ=255)                                                                                           gTCATAAATGTCGTCAGGGTCGTAATAATATTCTTTCACCAGCTTCCAAATACGGTATTGATCGGCTTGAACCACGGGATAATTGTAATCCGCCTGATCAATTTTGCCCTGCTCTTTGCCGCTCAGCACTCCGCCGACTGTCACATACAGGTCTTTCAGATTTTCCGGATCCACAAAGCCGTTTAAATAGGCGATAAAACGTCCGTTGCTATAGCTATCCACGATAGGTTTCGCGGAAAAATACGCCACCg                                                                                                                                             >  1:651100/1‑251 (MQ=255)                                                                                             cATAAATGTCGTCAGGGTCGTAATAATATTCTTTCACCAGCTTCCAAATACGGTATTGATCGGCTTGAACCACGGGATAATTGTAATCCGCCTGATCAATTTTGCCCTGCTCTTTGCCGCTCAGCACTCCGCCGACTGTCACATACAGGTCTTTCAGATTTTCCGGATCCACAAAGCCGTTTAAATAGGCGATAAAACGTCCGTTGCtat                                                                                                                                                                                    <  1:723027/210‑1 (MQ=255)                                                                                              aTAAATGTCGTCAGGGTCGTAATAATATTCTTTCACCAGCTTCCAAATACGGTATTGATCGGCTTGAACCACGGGATAATTGTAATCCGCCTGATCAATTTTGCCCTGCTCTTTGCCGCTCAGCACTCCGCCGACTGTCACATACAGGTCTTTCAGATTTTCCGGATCCACAAAGCCGTTTAAATAGGCGATAAAACGTCCGTTGCTATAGCTATCCACGATAGGTTTCGCGGAAAAATACGCCACCGttt                                                                                                                                          <  1:775349/251‑1 (MQ=255)                                                                                              aTAAATGTCGTCAGGGTCGTAATAATATTCTTTCACCAGCTTCCAAATACGGTATTGATCGGCTTGAACCACGGGATAATTGTAATCCGCCTGATCAATTTTGCCCTGCTCTTTGCCGCTCAGCACTCCGCCGACTGTCACATACAGGTCTTTCAGATTTTCCGGATCCACAAAGCCGTTTAAATAGGCGATAAAACGTCCGTTGCTATAGCTATCCACGATAGGTTTCGCGGAAAAATACGCCACCGttt                                                                                                                                          <  1:688771/251‑1 (MQ=255)                                                                                              aTAAATGTCGTCAGGGTCGTAATAATATTCTTTCACCAGCTTCCAAATACGGTATTGATCGGCTTGAACCACGGGATAATTGTAATCCGCCTGATCAATTTTGCCCTGCTCTTTGCCGCTCAGCACTCCGCCGACTGTCACATACAGGTCTTTCAGATTTTCCGGATCCACAAAGCCGTTTAAATAGGCGATAAAACGTCCGTTGCTATAGCTATCCACGATAGGTTTCGCGGAAAAATACGCCACCGttt                                                                                                                                          <  1:272145/251‑1 (MQ=255)                                                                                                   tGTCGTCAGGGTCGTAATAATATTCTTTCACCAGCTTCCAAATACGGTATTGATCGGCTTGAACCACGGGATAATTGTAATCCGCCTGATCAATTTTGCCCTGCTCTTTGCCGCTCAGCACTCCGCCGACTGTCACATACAGGTCTTTCAGATTTTCCGGATCCACAAAGCCGTTTAAATAGGCGATAAAACGGCCGTTGCTATAGCTATCCACGATAGGTTTCGCGGAAAAATACGCCACCGTTTGATTc                                                                                                                                     <  1:870312/251‑1 (MQ=255)                                                                                                      cgtcAGGGTCGTAATAATATTCTTTCACCAGCTTCCAAATACGGTATTGATCGGCTTGAACCACGGGATAATTGTAATCCGCCTGATCAATTTTGCCCTGCTCTTTGCCGCTCAGCACTCCGCCGACTGTCACATACAGGTCTTTCAGATTTTCCGGATCCACAAAGcc                                                                                                                                                                                                                    <  1:2053/169‑1 (MQ=255)                                                                                                            ggTCGTAATAATATTCTTTCACCAGCTTCCAAATACGGTATTGATCGGCTTGAACCACGGGATAATTGTAATCCGCCTGATCAATTTTGCCCTGCTCTTTGCCGCTCAGCACTCCGCCGACTGTCACATACAGGTCTTTCAGATTTTCCGGATCCACAAAGCCGTTTAAATAGGCGATAAAACGTCCGTTGCTATAGCTATCCACGATAGGTTTCGCGGAAAAATACGCCACCGTTTGATTCAATACTTc                                                                                                                             <  1:2291/250‑1 (MQ=255)                                                                                                                 taataaTATTCTTTCACCAGCTTCCAAATACGGTATTGATCGGCTTGAACCACGGGATAATTGTAATCCGCCTGATCAATTTTGCCCTGCTCTTTGCCGCTCAGCACTCCGCCGACTGTCACATACAGGTCTTTCAGATTTTCCGGATCCACAAAGCCGTTTAAATAGGCGATAAAACGTCCGTTGCTATAGCTATCCACGATAGGTTTCGCGGAAAAATAc                                                                                                                                                    >  1:516734/1‑222 (MQ=255)                                                                                                                   ataataTTCTTTCACCAGCTTCCAAATACGGTATTGATCGGCTTGAACCACGGGATAATTGTAATCCGCCTGATCAATTTTGCCCTGCTCTTTGCCGCTCAGCACTCCGCCGACTGTCACATACAGGTCTTTCAGATTTTCCGGATCCACAAAGCCGTTTAAATAGGCGATAAAACGTCCGTTGCTATAGCTATCCACGATAGGTTTCGCGGAAAAATACGCCACCGTTTGATTCAATACTTCAATTTTgg                                                                                                                     >  1:594449/1‑251 (MQ=255)                                                                                                                                    gCTTCCAAATACGGTATTGATCGGCTTGAACCACGGGATAATTGTAATCCGCCTGATCAATTTTGCCCTGCTCTTTGCCGCTCAGCACTCCGCCGACTGTCACATACAGGTCTTTCAGATTTTCCGGATCCACAAAGCCGTTTAAATAGGCGATAAAACGTCCGTTGCTATAGCTATCCACGATAGGTTTCGCGGAAAAATACGCCACCGTTTGATTCAATACTTCAATTTTGGTTTTATCTTTCAACGc                                                                                                     >  1:610601/1‑250 (MQ=255)                                                                                                                                       tCCAAATACGGTATTGATCGGCTTGAACCACGGGATAATTGTAATCCGCCTGATCAATTTTGCCCTGCTCTTTGCCGCTCAGCACTCCGCCGACTGTCACATACAGGTCTTTCAGATTTTCCGGATCCACAAAGc                                                                                                                                                                                                                     >  1:588986/1‑135 (MQ=255)                                                                                                                                            aTACGGTATTGATCGGCTTGAACCACGGGATAATTGTAATCCGCCTGATCAATTTTGCCCTGCTCTTTGCCGCTCAGCACTCCGCCGACTGTCACATACAGGTCTTTCAGATTTTCCGGATCCACAAAGCCGTTTAAATAGGCGATAAAACGTCCGTTGCTATAGCTATCCACGATAGGTTTCGCGGAAAAATACGCCACCGTTTGATTCAATACTTCAATTTTGGTTTTATCTTTCAACGCCTGTGCGGa                                                                                            >  1:365300/1‑251 (MQ=255)                                                                                                                                                 gTATTGATCGGCTTGAACCACGGGATAATTGTAATCCGCCTGATCAATTTTGCCCTGCTCTTTGCCGCTCAGCACTCCGCCGACTGTCACATACAGGTCTTTCAGATTTTCCGGATCCACAAAGCCGTTTAAATAGGCGATAAAACGTCCGTTGCTATAGc                                                                                                                                                                                 <  1:240960/161‑1 (MQ=255)                                                                                                                                                 gTATTGATCGGCTTGAACCACGGGATAATTGTAATCCGCCTGATCAATTTTGCCCTGCTCTTTGCCGCTCAGCACTCCGCCGACTGTCACATACAGGTCTTTCAGATTTTCCGGATCCACAAAGCCGTTTAAATAGGCGATAAAACGTCCGTTGCTATAGCTATCCACGATAGGTTTCGCGGAAAAATACGCCACCGTTTGATTCAATAc                                                                                                                                <  1:586953/210‑1 (MQ=255)                                                                                                                                                 gTATTGATCGGCTTGAACCACGGGATAATTGTAATCCGCCTGATCAATTTTGCCCTGCTCTTTGCCGCTCAGCACTCCGCCGACTGTCACATACAGGTCTTTCAGATTTTCCGGATCCACAAAGCCGTTTAAATAGGCGATAAAACGTCCGTTGCTATAGCTATCCACGATAGGTTTCGCGGAAAAATACGCCACCGTTTGATTCAATACTTCAATTTTGGTTTTATCTTTCAACGCCTGTGCGGAAAcc                                                                                        <  1:708244/250‑1 (MQ=255)                                                                                                                                                 gTATTGATCGGCTTGAACCACGGGATAATTGTAATCCGCCTGATCAATTTTGCCCTGCTCTTTGCCGCTCAGCACTCCGCCGACTGTCACATACAGGTCTTTCAGATTTTCCGGATCCACAAAGCCGTTTAAATAGGCGATAAAACGTCCGTTGCTATAGCTATCCACGATAGGTTTCGCGGAAAAATACGCCACCGTTTGATTCAATACTTCAATTTTGGTTTTATCTTTCAACGCCTGTGCGGAAACCa                                                                                       >  1:717899/1‑251 (MQ=255)                                                                                                                                                 gTATTGATCGGCTTGAACCACGGGATAATTGTAATCCGCCTGATCAATTTTGCCCTGCTCTTTGCCGCTCAGCACTCCGCCGACTGTCACATACAGGTCTTTCAGATTTTCCGGATCCACAAAGCCGTTTAAATAGGCGATAAAACGTCCGTTGCTATAGCTATCCACGATAGGTTTCGCGGAAAAATACGCCACCGTTTGATTCAATACTTCAATTTTGGTTTTATCTTTCAACGCCTGTGCGGAAACCa                                                                                       >  1:533115/1‑251 (MQ=255)                                                                                                                                                      gATCGGCTTGAACCACGGGATAATTGTAATCCGCCTGATCAATTTTGCCCTGCTCTTTGCCGCTCAGCACTCCGCCGACTGTCACATACAGGTCTTTCAGATTTTCCGGATCCACAAAGCCGTTTAAATAGGCGATAAAACGTCCGTTGCTATAGc                                                                                                                                                                                 >  1:281803/1‑156 (MQ=255)                                                                                                                                                          ggCTTGAACCACGGGATAATTGTAATCCGCCTGATCAATTTTGCCCTGCTCTTTGCCGCTCAGCACTCCGCCGACTGTCACATACAGGTCTTTCAGATTTTCCGGATCCACAAAGCCGTTTAAATAGGCGATAAAACGTCCGTTGCTATAGCTATCCACGATAGGTTTCGCGGAAAAATACGCCACCGTTTGATTCAATACTTCAATTTTGGTTTTATCTTTCAACGCCTGTGCGGAAACCACTTTgccg                                                                               >  1:80859/1‑250 (MQ=255)                                                                                                                                                          ggCTTGAACCACGGGATAATTGTAATCCGCCTGATCAATTTTGCCCTGCTCTTTGCCGCTCAGCACTCCGCCGACTGTCACATACAGGTCTTTCAGATTTTCCGGATCCACAAAGCCGTTTAAATAGGCGATAAAACGTCCGTTGCTATAGCTATCCACGATAGGTTTCACGGAAAAATACGCCACCGTTTGATTCAATACTTCAATTTTGGTTTTATCTTTCAACGCCTGTGCGGAAACCACTTTgccg                                                                               >  1:425766/1‑250 (MQ=255)                                                                                                                                                           gCTTGAACCACGGGATAATTGTAATCCGCCTGATCAATTTTGCCCTGCTCTTTGCCGCTCAGCACTCCGCCGACTGTCACATACAGGTCTTTCAGATTTTCCGGATCCACAAAGCCGTTTAAATAGGCGATAAAACGTCCGTTGCTATAGCTATCCACGATAGGTTTCGCGGAAAAATACGCCACCGTTTGAt                                                                                                                                       >  1:290325/1‑193 (MQ=255)                                                                                                                                                           gCTTGAACCACGGGATAATTGTAATCCGCCTGATCAATTTTGCCCTGCTCTTTGCCGCTCAGCACTCCGCCGACTGTCACATACAGGTCTTTCAGATTTTCCGGATCCACAAAGCCGTTTAAATAGGCGATAAAACGTCCGTTGCTATAGCTATCCACGATAGGTTTCGCGGAAAAATACGCCACCGTTTGATTCAATACTTCAATTTTGGTTTTATCTTTCAACGCCTGTGCGGAAACCACTTTgccgc                                                                              >  1:531/1‑250 (MQ=255)                                                                                                                                                                       ggATAATTGTAATCCGCCTGATCAATTTTGCCCTGCTCTTTGCCGCTCAGCACTCCGCCGACTGTCACATACAGGTCTTTCAGATTTTCCGGATCCACAAAGCCGTTTAAATAGGCGATAAAACGTCCGTTGCTATAGCTATCCACGATAGGt                                                                                                                                                                   >  1:457561/1‑153 (MQ=255)                                                                                                                                                                       ggATAATTGTAATCCGCCTGATCAATTTTGCCCTGCTCTTTGCCGCTCAGCACTCCGCCGACTGTCACATACAGGTCTTTCAGATTTTCCGGATCCACAAAGCCGTTTAAATAGGCGATAAAACGTCCGTTGCTATAGCTATCCACGATAGGTTTCGCGGAAAAATACGCCACCGTTTGATTCAATACTTCAATTTTGGTTTTATCTTTCAACGCCTGTGCGGAAACCACTTTGCCGCCCAAGCGCACttt                                                                 >  1:845272/1‑251 (MQ=255)                                                                                                                                                                       ggATAATTGTAATCCGCCTGATCAATTTTGCCCTGCTCTTTGCCGCTCAGCACTCCGCCGACTGTCACATACAGGTCTTTCAGATTTTCCGGATCCACAAAGCCGTTTAAATAGGCGATAAAACGTCCGTTGCTATAGCTATCCACGATAGGTTTCGCGGAAAAATACGCCACCGTTTGATTCAATACTTCAATTTTGGTTTTATCTTTCAACGCCTGTGCGGAAACCACTTTGCCGCCCAAGCGCACttt                                                                 >  1:357546/1‑251 (MQ=255)                                                                                                                                                                            aTTGTAATCCGCCTGATCAATTTTGCCCTGCTCTTTGCCGCTCAGCACTCCGCCGACTGTCACATACAGGTCTTTCAGATTTTCCGGATCCACAAAGc                                                                                                                                                                                                                     >  1:70874/1‑98 (MQ=255)                                                                                                                                                                            aTTGTAATCCGCCTGATCAATTTTGCCCTGCTCTTTGCCGCTCAGCACTCCGCCGACTGTCACATACAGGTCTTTCAGATTTTCCGGATCCACAAAGCCGTTTAAATAGGCGATAAAACGTCCGTTGCTATAGCTATCCACGATAGGTTTCGCGGAAAAATACGCCACCGTTTGATTCAATACTTCAATTTTGGTTTTATCTTTCAACGCCTGTGCGGAAACCACTTTGCCGCCCAAGCGCACTTTCAgg                                                             >  1:598867/1‑250 (MQ=255)                                                                                                                                                                            aTTGTAATCCGCCTGATCAATTTTGCCCTGCTCTTTGCCGCTCAGCACTCCGCCGACTGTCACATACAGGTCTTTCAGATTTTCCGGATCCACAAAGCCGTTTAAATAGGCGATAAAACGTCCGTTGCTATAGCTATCCACGATAGGTTTCGCGGAAAAATACGCCACCGTTTGATTCAATACTTCAATTTTGGTTTTATCTTTCAACGCCTGTGCGGAAACCACTTTGCCGCCCAAGCGCACTTTCAgg                                                             >  1:414764/1‑250 (MQ=255)                                                                                                                                                                             ttGTAATCCGCCTGATCAATTTTGCCCTGCTCTTTGCCGCTCAGTACTCCGCCGACTGTCACATACAGGTCTTTCAGATTTTCCGGATCCACAAAGCCGTTTAAATAGGCGATAAAACGTCCGTTGCTATAGCTATCCACGATAGGTTTCGCGGAAAAATACGCCACCGTTTGATTCAATACTTCAATTTTGGTTTTATCTTTCAACGCCTGTGCGGAAACCACTTTGCCGCCCAAGCGCACTTTCAGGCa                                                           >  1:356716/1‑251 (MQ=255)                                                                                                                                                                             ttGTAATCCGCCTGATCAATTTTGCCCTGCTCTTTGCCGCTCAGCACTCCGCCGACTGTCACATACAGGTCTTTCAGATTTTCCGGATCCACAAAGCCGTTTAAATAGGCGATAAAACGTCCGTTGCTATAGCTATCCACGATAGGTTTCGCGGAAAAATACGCCACCGTTTGATTCAATACTTCAATTTTGGTTTTATCTTTCAACGCCTGTGCGGAAACCACTTTGCCGCCCAAGCGCACTTTCAGGCa                                                           >  1:151047/1‑251 (MQ=255)                                                                                                                                                                                                    gCCCTGCTCTTTGCCGCTCAGCACTCCGCCGACTGTCACATACAGGTCTTTCAGATTTTCCGGATCCACAAAGCCGTTTAAATAGGCGATAAAACGTCCGTTGCTATAGc                                                                                                                                                                                 <  1:726408/110‑1 (MQ=255)                                                                                                                                                                                                    gCCCTGCTCTTTGCCGCTCAGCACTCCGCCGACTGTCACATACAGGTCTTTCAGATTTTCCGGATCCACAAAGCCGTTTAAATAGGCGATAAAACGTCCGTTGCTATAGCTATCCACGATAGGTTTCGCGGAAAAATACGCCACCGTTTGATTCAATACTTCAATTTTGGTTTTATCTTTCAACGCCTGTGCGGAAACCACTTTGCCGCCCAAGCGCACTTTCAGGCAGCGGCAATc                                                  >  1:471378/1‑237 (MQ=255)                                                                                                                                                                                                         gCTCTTTGCCGCTCAGCACTCCGCCGACTGTCACATACAGGTCTTTCAGATTTTCCGGATCCACAAAGCCGTTTAAATAGGCGATAAAACGTCCGTTGCTATAGCTATCCACGATAGGTTTCGCGGAAAAATACGCCACCGTTTGATTCAATACTTCAATTTTGGTTTTATCTTTCAACGCCTGTGCGGAAACCACTTTGCCGCCCAAGCGCACTTTCAGGCAGCGGCAATCGTAATCCTGCGCCTGcaca                               >  1:180773/1‑251 (MQ=255)                                                                                                                                                                                                              ttGCCGCTCAGCACTCCGCCGACTGTCACATACAGGTCTTTCAGATTTTCCGGATCCACAAAGCCGTTTAAATAGGCGATAAAACGTCCGTTGCTATAGCTATCCACGATAGGTTTCGCGGAAAAATACGCCACCGTTTGATTCAATACTTCAATTTTGGTTTTATCTTTCAACGCCTGTGCGGAAACCACTTTGCCGCCCAAGCGCACTTTCAGGCAGCGGCAATCGTAATCCTGCGCCTGCACAttttt                          >  1:159840/1‑251 (MQ=255)                                                                                                                                                                                                                      cAGCACTCCGCCGACTGTCACATACAGGTCTTTCAGATTTTCCGGATCCACAAAGCCGTTTAAATAGGCGATAAAACGTCCGTTGCTATAGc                                                                                                                                                                                 <  1:263042/92‑1 (MQ=255)                                                                                                                                                                                                                                    cTGTCACATACAGGTCTTTCAGATTTTCCGGATCCACAAAGCCGTTTAAATAGGCGATAAAACGTCCGTTGCTATAGCTATCCACGATAGGTTTCGCGGAAAAATACGCCACCGTTTGATTCAATACTTCAATTTTGGTTTTATCTTTCAACGCCTGTGCGGAAACCACATTGCCGCCCAAGCGCACTTTCAGGCAGCGGCAATCGTAATCCTGCGCCTGcac                                >  1:557586/1‑223 (MQ=255)                                                                                                                                                                                                                                      gTCACATACAGGTCTTTCAGATTTTCCGGATCCACAAAGCCGTTTAAATAGGCGATaa                                                                                                                                                                                                   >  1:650411/1‑58 (MQ=255)                                                                                                                                                                                                                                      gTCACATACAGGTCTTTCAGATTTTCCGGATCCACAAAGCCGTTTAAATAGGCGATAAAACGTCCGTTGCTATAGCTATCCACGATAGGTTTCGCGGAAAAATACGCCACCGTTTGATTCAATACTTCAATTTTGGTTTTATCTTTCAACGCCTGTGCGGAAACCACTTTGCCGCCCAAGCGCACTTTCAGGCAGCGGCAATCGTAATCCTGCGCCTGCACATTTTTCAGATTTAACATTTCGAACTGttt  >  1:709117/1‑251 (MQ=255)                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                                  GGTTAATTAATATAAATTATAGCGCACGCGCGGTTCAGCCCAAAAGAAGCGGGACGAAAATCCCCAGCCGTAAGGGTAAAACGGTGCGTAGTCATAAATGTCGTCAGGGTCGTAATAATATTCTTTCACCAGCTTCCAAATACGGTATTGATCGGCTTGAACCACGGGATAATTGTAATCCGCCTGATCAATTTTGCCCTGCTCTTTGCCGCTCAGCACTCCGCCGACTGTCACATACTGGTCTTTCAGATTTTCCGGATCCACAAAGCCGTTTAAATAGGCGATAAAACGTCCGTTGCTATAGCTATCCACGATAGGTTTCGCGGAAAAATACGCCACCGTTTGATTCAATACTTCAATTTTGGTTTTATCTTTCAACGCCTGTGCGGAAACCACTTTGCCGCCCAAGCGCACTTTCAGGCAGCGGCAATCGTAATCCTGCGCCTGCACATTTTTCAGATTTAACATTTCGAACTGTTT  >  1/1809826‑1810305

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑