Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,809,688	T→G	intergenic (+14761/‑124643)	unknown → / → unknown	tRNA‑Leu‑CAA/tRNA‑Asp‑GTC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,809,688	0	T	G	100.0%	180.3 / NA	66	intergenic (+14761/‑124643)	unknown/unknown	tRNA‑Leu‑CAA/tRNA‑Asp‑GTC
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (35/31);  total (35/31)

CGCGGCGTATAAAGCGGGTTCACATTGACCACCACCAACCCAGCACGCAACACCCCGAACAACGCAATGGGATATTGCAGCAAATTCGGCATCATTAATGCCACGCGATCGCCGCGCTCCAATTTCAATTCATTTTGCAAATAGGCAGCGAAAGCACGGCTACGTTCTTCCAATTTGCGAAACGTCAACACCTTGCCCATATTAATATAAGCGGCACGATCGGGATGTTCTCGCACCGCTTTTTCAAACATATCTAAAATCGAATCGTATTCCGAGGTGTCTAATTGGCGCTCCGCACCTTCCGGATAATTTTTAAACCAAAGTTTTTCCATTGCCGTTCCTTAGGGAAGTTTGGTTATTGTTAATCGGGTTAATTAATATAAATTATAGCGCACGCGCGGTTCAGCCCAAAAGAAGCGGGACGAAAATCCCCAGCCGTAAGGGTAAAACGGTGCGTAGTCATAAATGTCGTCAGGG  >  1/1809458‑1809934                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                       cgcgGCGTATAAAGCGGGTTCACATTGCCCACCACCAACCCATCACGCAACACCCCGAACAACGCAATGGGATATTGCAGCAAATTCGGCATCATTAATGCCACGCGATCGCCGCGCTCCAATTTCAATTCATTTTGCAAATAGGCAGCGAAAGCACGGCTACGTTCTTCCAATTTGCGAAGCGTCAACACCTTGCCCATATTAATATAAGCGGCACGATCGGGATGTTCGCGCACCGCTTTTTCAAACa                                                                                                                                                                                                                                     <  1:257194/250‑1 (MQ=255)     gcgTATAAAGCGGGTTCACATTGACCACCACCAACCCAGCACGCAACACCCCGAACAACGCAATGGGATATTGCAGCAAATTCGGCATCATTAATGCCACGCGATCGCCGCGCTCCAATTTCAATTCATTTTGCAAATAGGCAGCGAAAGCACGGCTACGTTCTTCCAATTTGCGAAACGTCAACACCTTGCCCATATTAATATAAGCGGCACGATCGGGATGTTCGCGCACCGCTTTTTCAAACatat                                                                                                                                                                                                                                  >  1:839580/1‑249 (MQ=255)     gcgTATAAAGCGGGTTCACATTGACCACCACCAACCCAGCACGCAACACCCCGAACAACGCAATGGGATATTGCAGCAAATTCGGCATCATTAATGCCACGCGATCGCCGCGCTCCAATTTCAATTCATTTTGCAAATAGGCAGCGAAAGCACGGCTACGTTCTTCCAATTTGCGAAACGTCAACACCTTGCCCATATTAATATAAGCGGCACGATCGGGATGTTCGCGCACCGCTTTTTCAAACatat                                                                                                                                                                                                                                  >  1:666212/1‑249 (MQ=255)       gTATAAAGCGGGTTCACATTGACCACCACCAACCCAGCACGCAACACCCCGAACAACGCAATGGGATATTGCAGCAAATTCGGCATCATTAATGCCACGCGATCGCCGCGCTCCAATTTCAATTCATTTTGCAAATAGGCAGCGAAAGCACGGCTACGTTCTTCCAATTTGCGAAACGTCAACACCTTGCCCATATTAATATAAGCGGCACGATCGGGATGTTCGCGCACCGCttttt                                                                                                                                                                                                                                           >  1:626824/1‑238 (MQ=255)       gTATAAAGCGGGTTCACATTGACCACCACCAACCCAGCACGCAACACCCCGAACAACGCAATGGGATATTGCAGCAAATTCGGCATCATTAATGCCACGCGATCGCCGCGCTCCAATTTCAATTCATTTTGCAAATAGGCAGCGAAAGCACGGCTACGTTCTTCCAATTTGCGAAACGTCAACACCTTGCCCATATTAATATAAGCGGCACGATCGGGATGTTCGCGCACCGCTTTTTCAAACATATCTaa                                                                                                                                                                                                                              >  1:838774/1‑251 (MQ=255)                 ggTTCACATTGACCACCACCAACCCAGCACGCAACACCCCGAACAACGCAATGGGATATTGCAGCAAATTCGGCATCATTAATGCCACGCGATCGCCGCGCTCCAATTTCAATTCATTTTGCAAATAGGCAGCGAAAGCACGGCTACGTTCTTCCAATTTGCGAAACGTCAACACCTTGCCCATATTAATATAAGCGGCACGATCGGGATGTTCGCGCACCGCttttt                                                                                                                                                                                                                                           <  1:210965/228‑1 (MQ=255)                 ggTTCACATTGACCACCACCAACCCAGCACGCAACACCCCGAACAACGCAATGGGATATTGCAGCAAATTCGGCATCATTAATGCCACGCGATCGCCGCGCTCCAATTTCAATTCATTTTGCAAATAGGCAGCGAAAGCACGGCTACGTTCTTCCAATTTGCGAAACGTCAACACCTTGCCCATATTAATATAAGCGGCACGATCGGGATGTTCGCGCACCGCTTTTTCAAACATATCTAAaatcgaatcg                                                                                                                                                                                                                    >  1:231005/1‑251 (MQ=255)                      acaTTGACCACCACCAACCCAGCACGCAACACCCCGAACAACGCAATGGGATATTGCAGCAAATTCGGCATCATTAATGCCACGCGATCGCCGCGCTCCAATTTCAATTCATTTTGCAAATAGGCAGCGAAAGCACGGCTACGTTCTTCCAATTTGCGAAACGTCAACACCTTGCCCATATTAATATAAGCGGCACGATCGGGATGTTCGCGCACCGCTTTTTCAAACATATCTAAAATCGAATCGTAtt                                                                                                                                                                                                                >  1:627613/1‑250 (MQ=255)                           gACCACCACCAACCCAGCACGCAACACCCCGAACAACGCAATGGGATATTGCAGCAAATTCGGCATCATTAATGCCACGCGATCGCCGCGCTCCAATTTCAATTCATTTTGCAAATAGGCAGCGAAAGCACGGCTACGTTCTTCCAATTTGCGAAACGTCAACACCTTGCCCATATTAATATAAGCGGCACGATCGGGATGTTCGCGCACCGCTTTTTCAAACATATCTAAAATCGAATCGTATTCCGAgg                                                                                                                                                                                                          >  1:96922/1‑251 (MQ=255)                            accaccaccAACCCAGCACGCAACACCCCGAACAACGCAATGGGATATTGCAGCAAATTCGGCATCATTAATGCCACGCGATCGCCGCGCTCCAATTTCAATTCATTTTGCAAATAGGCAGCGAAAGTACGGCTACGTTCTTCCAATTTGCGAAACGTCAACACCTTGCCCATATTAATATAAGCGGCACGATCGGGATGTTCGCGCACCGCTTTTTCAAACATATCTAAAATCGAATCGTATTCCGAgg                                                                                                                                                                                                          <  1:229784/250‑1 (MQ=255)                                ccaccaACCCAGCACGCAACACCCCGAACAACGCAATGGGATATTGCAGCAAATTCGGCATCATTAATGCCACGCGATCGCCGCGCTCCAATTTCAATTCATTTTGCAAATGGGCAGCGAAAGCACGGCTACGTTCTTCCAATTTGCGAAACGTCAACACCTTGCCCATATTAATATAAGCGGCACGATCGGGATGTTCGCGCACCGCTTTTTCAAACATATCTAAAATCGAATCGTATTCCGAGgtgt                                                                                                                                                                                                       >  1:35342/1‑249 (MQ=255)                                ccaccaACCCAGCACGCAACACCCCGAACAACGCAATGGGATATTGCAGCAAATTCGGCATCATTAATGCCACGCGATCGCCGCGCTCCAATTTCAATTCATTTTGCAAATAGGCAGCGAAAGCACGGCTACGTTCTTCCAATTTGCGAAACGTCAACACCTTGCCCATATTAATATAAGCGGCACGATCGGGATGTTCGCGCACCGCTTTTTCAAACATATCTAAAATCGAATCGTATTCCGAGgtgt                                                                                                                                                                                                       >  1:483744/1‑249 (MQ=255)                                      aCCCAGCACGCAACACCCCGAACAACGCAATGGGATATTGCAGCAAATTCGGCATCATTAATGCCACGCGATCGCCGCGCTCCAATTTCAATTCATTTTGCAAATAGGCAGCGAAAGCACGGCTACGTTCTTCCAATTTGCGAAACGTCAACACCTTGCCCATATTAATGTAAGCGGCACGATCGGGATGTTCGCGCACCGCTTTTTCAAACATATCTAAAATCGAATCGTATTCCGAGGTGTCTAATTgg                                                                                                                                                                                               >  1:448483/1‑251 (MQ=255)                                      aCCCAGCACGCAACACCCCGAACAACGCAATGGGATATTGCAGCAAATTCGGCATCATTAATGCCACGCGATCGCCGCGCTCCAATTTCAATTCATTTTGCAAATAGGCAGCGAAAGCACGGCTACGTTCTTCCAATTTGCGAAACGTCAACACCTTGCCCATATTAATATAAGCGGCACGATCGGGATGTTCGCGCACCGCTTTTTCAAACATATCTAAAATCGAATCGTATTCCGAGGTGTCTAATTgg                                                                                                                                                                                               <  1:633859/251‑1 (MQ=255)                                                    acCCCGAACAACGCAATGGGATATTGCAGCAAATTCGGCATCATTAATGCCACGCGATCGCCGCGCTCCAATTTCAATTCATTTTGCAAATAGGCAGCGAAAGCACGGCTACGTTCTTCCAATTTGCGAAACGTCAACACCTTGCCCATATTAATATAAGCGGCACGATCGGGATGTTCGCGCACCGCTTTTTCAAACATATCTAAAATCGAATCGTATTCCGAGGTGTCTAATTGGCGCTCCGCACCtt                                                                                                                                                                                  >  1:530831/1‑250 (MQ=255)                                                                 cAATGGGATATTGCAGCAAATTCGGCATCATTAATGCCACGCGATCGCCGCGCTCCAATTTCAATTCATTTTGCAAATAGGCAGCGAAAGCACGGCTACGTTCTTCCAATTTGCGAAACGTCAACACCTTGCCCATATTAATATAAGCGGCACGATCGGGATGTTCGCGCAc                                                                                                                                                                                                                                                   <  1:885463/172‑1 (MQ=255)                                                                   aTGGGATATTGCAGCAAATTCGGCATCATTAATGCCACGCGATCGCCGCGCTCCAATTTCAATTCATTTTGCAAATAGGCAGCGAAAGCACGGCTACGTTCTTCCAATTTGCGAAACGTCAACACCTTGCCCATATTAATATAAGCGGCACGATCGGGATGTTCGCGCACCGCTTTTTCAAACATATCTAAAATCGAATCGTATTCCGAGGTGTCTAATTGGCGCTCCGCACCTTCCGGATAATTTTTaaa                                                                                                                                                                  >  1:200211/1‑251 (MQ=255)                                                                      ggATATTGCAGCAAATTCGGCATCATTAATGCCACGCGATCGCCGCGCTCCAATTTCAATTCATTTTGCAAATAGGCAGCGAAAGCACGGCTACGTTCTTCCAATTTGCGAAACGTCAACACCTTGCCCATATTAATATAAGCGGCACGATCGGGATGTTCGCGCACCGCTTTTTCAAACATATCTAAAATCGAATCGTATTCCGAGGTGTCTAATTGGCGCTCCGCACCTTCCGGATAATTTTTAAACCa                                                                                                                                                               >  1:309892/1‑251 (MQ=255)                                                                                            tcatTAATGCCACGCGATCGCCGCGCTCCAATTTCAATTCATTTTGCAAATAGGCAGCGAAAGCACGGCTACGTTCTTCCAATTTGCGAAACGTCAACACCTTGCCCATATTAATATAAGCGGCACGATCGGGATGTTCGCGCACCGCttttt                                                                                                                                                                                                                                           >  1:287420/1‑153 (MQ=255)                                                                                            tcatTAATGCCACGCGATCGCCGCGCTCCAATTTCAATTCATTTTGCAAATAGGCAGCGAAAGCACGGCTACGTTCTTCCAATTTGCGAAACGTCAACACCTTGCCCATATTAATATAAGCGGCACGATCGGGATGTTCGCGCACCGCTTTTTCAAACATATCTAAAATCGAATCGTATTCCGAGGTGTCTAATTGGCGCTCCGCACCTTCCGGATAATTTTTAAACCAAAGTTTTTcc                                                                                                                                                     >  1:758193/1‑239 (MQ=255)                                                                                            tcatTAATGCCACGCGATCGCCGCGCTCCAATTTCAATTCATTTTGCAAATAGGCAGCGAAAGCACGGCTACGTTCTTCCAATTTGCGAAACGTCAACACCTTGCCCATATTAATATAAGCGGCACGATCGGGATGTTCGCGCACCGCTTTTTCAAACATATCTAAAATCGAATCGTATTCCGAGGTGTCTAATTGGCGCTCCGCACCTTCCGGATAATTTTTAAACCAAAGTTTTTCCATTGCCGTTc                                                                                                                                           >  1:689462/1‑249 (MQ=255)                                                                                            tcatTAATGCCACGCGATCGCCGCGCTCCAATTTCAATTCATTTTGCAAATAGGCAGCGAAAGCACGGCTACGTTCTTCCAATTTGCGAAACGTCAACACCTTGCCCATATTAATATAAGCGGCACGATCGGGATGTTCGCGCACCGCTTTTTCAAACATATCTAAAATCGAATCGTATTCCGAGGTGTCTAATTGGCGCTCCGCACCTTCCGGATAATTTTTAAACCAAAGTTTTTCCATTGCCGTTc                                                                                                                                           >  1:499795/1‑249 (MQ=255)                                                                                            tcatTAATGCCACGCGATCGCCGCGCTCCAATTTCAATTCATTTTGCAAATAGGCAGCGAAAGCACGGCTACGTTCTTCCAATTTGCGAAACGTCAACACCTTGCCCATATTAATATAAGCGGCACGATCGGGATGTTCGCGCACCGCTTTTTCAAACATATCTAAAATCGAATCG‑ATTCCGAGGTGTCTAATTGGCGCTCCGCACCTTCCGGATAATTTTTAAACCAAAGTTTTTCCATTGCCGTTCCtt                                                                                                                                        <  1:824185/251‑1 (MQ=255)                                                                                             catTAATGCCACGCGATCGCCGCGCTCCAATTTCAATTCATTTTGCAAATAGGCAGCGAAAGCACGGCTACGTTCTTCCAATTTGCGAAACGTCAACACCTTGCCCATATTAATATAAGCGGCACGATCGGGATGTTCGCGCACCGCTTTTTCAAACATATCTaa                                                                                                                                                                                                                              >  1:870008/1‑165 (MQ=255)                                                                                             catTAATGCCACGCGATCGCCGCGCTCCAATTTCAATTCATTTTGCAAATAGGCAGCGAAAGCACGGCTACGTTCTTCCAATTTGCGAAACGTCAACACCTTGCCCATATTAATATAAGCGGCACGATCGGGATGTTCGCGCACCGCTTTTTCAAACATATCTAAAATCGAATCGTATTCCGAGGTGTCTaa                                                                                                                                                                                                   >  1:166067/1‑192 (MQ=255)                                                                                             catTAATGCCACGCGATCGCCGCGCTCCAATTTCAATTCATTTTGCAAATAGGCAGCGAAAGCACGGCTACGTTCTTCCAATTTGCGAAACGTCAACACCTTGCCCATATTAATATAAGCGGCACGATCGGGATGTTCGCGCACCGCTTTTTCAAACATATCTAAAATCGAATCGTATTCCGAGGTGTCTAATTGGCGCTCCGCACCTTCCGGATAATTTTTAAACCAAAGTTTTTCCATTGCCGTTc                                                                                                                                           >  1:696409/1‑248 (MQ=255)                                                                                             catTAATGCCACGCGATCGCCGCGCTCCAATTTCAATTCATTTTGCAAATAGGCAGCGAAAGCACGGCTACGTTCTTCCAATTTGCGAAACGTCAACACCTTGCCCATATTAATATAAGCGGCACGATCGGGATGTTCGCGCACCGCTTTTTCAAACATATCTAAAATCGAATCGTATTCCGAGGTGTCTAATTGGCGCTCCGCACCTTCCGGATAATTTTTAAACCAAAGTTTTTCCATTGCCGTTCCtt                                                                                                                                        >  1:591901/1‑251 (MQ=255)                                                                                             catTAATGCCACGCGATCGCCGCGCTCCAATTTCAATTCATTTTGCAAATAGGCAGCGAAAGCACGGCTACGTTCTTCCAATTTGCGAAACGTCAACACCTTGCCCATATTAATATAAGCGGCACGATCGGGATGTTCGCGCACCGCTTTTTCAAACATATCTAAAATCGAATCGTATTCCGAGGTGTCTAATTGGCGCTCCGCACCTTCCGGATAATTTTTAAACCAAAGTTTTTCCATTGCCGTTCCtt                                                                                                                                        >  1:728321/1‑251 (MQ=255)                                                                                             catTAATGCCACGCGATCGCCGCGCTCCAATTTCAATTCATTTTGCAAATAGGCAGCGAAAGCACGGCTACGTTCTTCCAATTTGCGAAACGTCAACACCTTGCCCATATTAATATAAGCGGCACGATCGGGATGTTCGCGCACCGCTTTTTCAAACATATCTAAAATCGAATCGTATTCCGAGGTGTCTAATTGGCGCTCCGCACCTTCCGGATAATTTTTAAACCAAAGTTTTTCCATTGCCGTTCCtt                                                                                                                                        >  1:64981/1‑251 (MQ=255)                                                                                                 aaTGCCACGCGATCGCCGCGCTCCAATTTCAATTCATTTTGCAAATAGGCAGCGAAAGCACGGCTACGTTCTTCCAATTTGCGAAACGTCAACACCTTGCCCATATTAATATAAGCGGCACGATCGGGATGTTCGCGCACCGCTTTTTCAAACATATCTAAAATCGAATCGTATTCCGAGGTGTCTAATTGGCGCTCCGCACCTTCCGGATAATTTTTAAACCAAAGTTTTTCCATTGCCGTTCCTTAggg                                                                                                                                    <  1:23047/251‑1 (MQ=255)                                                                                                 aaTGCCACGCGATCGCCGCGCTCCAATTTCAATTCATTTTGCAAATAGGCAGCGAAAGCACGGCTACGTTCTTCCAATTTGCGAAACGTCAACACCTTGCCCATATTAATATAAGCGGCACGATCGGGATGTTCGCGCACCGCTTTTTCAAACATATCTAAAATCGAATCGTATTCCGAGGTGTCTAATTGGCGCTCCGCACCTTCCGGATAATTTTTAAACCAAAGTTTTTCCATTGCCGTTCCTTAggg                                                                                                                                    <  1:98915/251‑1 (MQ=255)                                                                                                  aTGCCACGCGATCGCCGCGCTCCAATTTCAATTCATTTTGCAAATAGGCAGCGAAAGCACGGCTACGTTCTTCCAATTTGCGAAACGTCAACACCTTGCCCATATTAATATAAGCGGCACGATCGGGATGTTCGCGCACCGCTTTTTCAAACATATCTAAAATCGAATCGTATTCCGAGGTGTCTAATTGGCGCTCCGCACCTTCCGGATAATTTTTAAACCAAAGTTTTTCCATTGCCGTTCCTTAGGGa                                                                                                                                   <  1:409795/251‑1 (MQ=255)                                                                                                  aTGCCACGCGATCGCCGCGCTCCAATTTCAATTCATTTTGCAAATAGGCAGCGAAAGCACGGCTACGTTCTTCCAATTTGCGAAACGTCAACACCTTGCCCATATTAATATAAGCGGCACGATCGGGATGTTCGCGCACCGCTTTTTCAAACATATCTAAAATCGAATCGTATTCCGAGGTGTCTAATTGGCGCTCCGCACCTTCCGGATAATTTTTAAACCAAAGTTTTTCCATTGCCGTTCCTTAGGGa                                                                                                                                   <  1:443640/251‑1 (MQ=255)                                                                                                            aTCGCCGCGCTCCAATTTCAATTCATTTTGCAAATAGGCAGCGAAAGCACGGCTACGTTCTTCCAATTTGCGAAACGTCAACACCTTGCCCATATTAATATAAGCGGCACGATCGGGATGTTCGCGCACCGCTTTTTCAAACATATCTAAAATCGAATCGTATTCCGAGGTGTCTAATTGGCGCTCCGCACCTTCCGGATAATTTTTAAACCAAAGTTTTTCCATTGCCGTTCCTTAGGGAAGTTTGGTTa                                                                                                                         <  1:816320/251‑1 (MQ=255)                                                                                                                     cTCCAATTTCAATTCATTTTGCAAATAGGCAGCGAAAGCACGGCTACGTTCTTCCAATTTGCGAAACGTCAACACCTTGCCCATATTAATATAAGCGGCACGATCGGGATGTTCGCGCACCGCTTTTTCAAACATATCTAAaat                                                                                                                                                                                                                           <  1:583341/144‑1 (MQ=255)                                                                                                                     cTCCAATTTCAATTCATTTTGCAAATAGGCAGCGAAAGCACGGCTACGTTCTTCCAATTTGCGAAACGTCAACACCTTGCCCATATTAATATAAGCGGCACGATCGGGATGTTCGCGCACCGCTTTTTCAAACATATCTAAAATCGAATCGTATTCCGAGGTGTCTAATTGGCGCTCCGCACCTTCCGGATAATTTTTAAACCAAAGTTTTTCCATTGCCGTTCCTTAGGGa                                                                                                                                   <  1:802035/232‑1 (MQ=255)                                                                                                                     cTCCAATTTCAATTCATTTTGCAAATAGGCAGCGAAAGCACGGCTACGTTCTTCCAATTTGCGAAACGTCAACACCTTGCCCATATTAATATAAGCGGCACGATCGGGATGTTCGCGCACCGCTTTTTCAAACATATCTAAAATCGAATCGTATTCCGAGGTGTCTAATTGGCGCTCCGCACCTTCCGGATAATTTTTAAACCAAAGTTTTTCCATTGCCGTTCCTTAGGGa                                                                                                                                   <  1:13511/232‑1 (MQ=255)                                                                                                                                   cATTTTGCAAATAGGCAGCGAAAGCACGGCTACGTTCTTCCAATTTGCGAAACGTCAACACCTTGCCCATATTAATATAAGCGGCACGATCGGGATGTTCGCGCACCGCTTTTTCAAACATATCTAAAATCGAATCGTATTCCGAGGTGTCTAATTGGCGCTCCGCACCTTCCGGATAATTTTTAAACCAAAGTTTTTCCATTGCCGTTCCTTAGGGAAGTTTGGTTATTGTTAATCGGGTTAATTAatat                                                                                                  <  1:816207/251‑1 (MQ=255)                                                                                                                                   cATTTTGCAAATAGGCAGCGAAAGCACGGCTACGTTCTTCCAATTTGCGAAACGTCAACACCTTGCCCATATTAATATAAGCGGCACGATCGGGATGTTCGCGCACCGCTTTTTCAAACATATCTAAAATCGAATCGTATTCCGAGGTGTCTAATTGGCGCTCCGCACCTTCCGGATAATTTTTAAACCAAAGTTTTTCCATTGCCGTTCCTTAGGGAAGTTTGGTTATTGTTAATCGGGTTAATTAatat                                                                                                  <  1:207556/251‑1 (MQ=255)                                                                                                                                        tGCAAATAGGCAGCGAAAGCACGGCTACGTTCTTCCAATTTGCGAAACGTCAACACCTTGCCCATATTAATATAAGCGGCACGATCGGGATGTTCGCGCACCGCTTTTTCAAACATATCTAAAATCGAATCGTATTCCGAGGTGTCTAATTGGCGCTCCGCACCTTCCGGATAATTTTTAAACCAAAGTTTTTCCATTGCCGTTCCTTAGGGAAGTTTGGTTATTGTTAATCGGGTTAATTAATATAAAtt                                                                                             <  1:614456/251‑1 (MQ=255)                                                                                                                                                           cACGGCTACGTTCTTCCAATTTGCGAAACGTCAACACCTTGCCCATATTAATATAAGCGGCACGATCGGGATGTTCGCGCACCGCTTTTTCAAACATATCTAAAATCGAATCGTATTCCGAGGTGTCTAATTGGCGCTCCGCACCTTCCGGATAATTTTTAAACCAAAGTTTTTCCATTGCCGTTCCTTAGGGAAGTTTGGTTATTGTTAATCGGGTTAATTAATATAAATTATAGCGCACGCGCGGTTc                                                                           <  1:607847/250‑1 (MQ=255)                                                                                                                                                                  aCGTTCTTCCAATTTGCGAAACGTCAACACCTTGCCCATATTAATATAAGCGGCACGATCGGGATGTTCGCGCACCGCTTTTTCAAACATATCTaa                                                                                                                                                                                                                              <  1:282063/96‑1 (MQ=255)                                                                                                                                                                                       cGTCAACACCTTGCCCATATTAATATAAGCGGCACGATCGGGATGTTCGCGCACCGCTTTTTCAAACATATCTAAAATCGAATCGTATTCCGAGGTGTCTAATTGGCGCTCCGCACCTTCCGGATAATTTTTAAACCAAAGTTTTTCCATTGCCGTTCCTTAGGGAAGTTTGGTTATTGTTAATCGGGTTAATTAATATAAATTATAg                                                                                         >  1:614631/1‑208 (MQ=255)                                                                                                                                                                                       cGTCAACACCTTGCCCATATTAATATAAGCGGCACGATCGGGATGTTCGCGCACCGCTTTTTCAAACATATCTAAAATCGAATCGTATTCCGAGGTGTCTAATTGGCGCTCCGCACCTTCCGGATAATTTTTAAACCAAAGTTTTTCCATTGCCGTTCCTTAGGGAAGTTTGGTTATTGTTAATCGGGTTAATTAATATAAATTATAGCGCACGCGCGGTTCAGCCCAAAAGAAGCGGGACGAAAATccc                                               >  1:526814/1‑250 (MQ=255)                                                                                                                                                                                       cGTCAACACCTTGCCCATATTAATATAAGCGGCACGATCGGGATGTTCGCGCACCGCTTTTTCAAACATATCTAAAATCGAATCGTATTCCGAGGTGTCTAATTGGCGCTCCGCACCTTCCGGATAATTTTTAAACCAAAGTTTTTCCATTGCCGTTCCTTAGGGAAGTTTGGTTATTGTTAATCGGGTTAATTAATATAAATTATAGCGCACGCGCGGTTCAGCCCAAAAGAAGCGGGACGAAAATccc                                               >  1:45654/1‑250 (MQ=255)                                                                                                                                                                                       cGTCAACACCTTGCCCATATTAATATAAGCGGCACGATCGGGATGTTCGCGCACCGCTTTTTCAAACATATCTAAAATCGAATCGTATTCCGAGGTGTCTAATTGGCGCTCCGCACCTTCCGGATAATTTTTAAACCAAAGTTTTTCCATTGCCGTTCCTTAGGGAAGTTTGGTTATTGTTAATCGGGTTAATTAATATAAATTATAGCGCACGCGCGGTTCAGCCCAAAAGAAGCGGGACGAAAATccc                                               >  1:208328/1‑250 (MQ=255)                                                                                                                                                                                                 ttGCCCATATTAATATAAGCGGCACGATCGGGATGTTCGCGCACCGCTTTTTCAAACATATCTAAAATCGAATCGTATTCCGAGGTGTCTAATTGGCGCTCCGCACCTTCCGGATAATTTTTAAACCAAAGTTTTTCCATTGCCGTTCCTTAGGGAAGTTTGGTTATTGTTAATCGGGTTAATTAATATAAATTATAGCGCACGCGCGGTTCAGCCCAAAAGAAGCGGGACGAAAATCCCCAGCCGTAAgg                                    >  1:814534/1‑251 (MQ=255)                                                                                                                                                                                                   gCCCATATTAATATAAGCGGCACGATCGGGATGTTCGCGCACCGCTTTTTCAAACATATCTAAAATCGAATCGTATTCCGAGGTGTCTAATTGGCGCTCCGCACCTTCCGGATAATTTTTAAACCAAAGTTTTTCCATTGCCGTTCCTTAGGGa                                                                                                                                   <  1:139038/154‑1 (MQ=255)                                                                                                                                                                                                   gCCCATATTAATATAAGCGGCACGATCGGGATGTTCGCGCACCGCTTTTTCAAACATATCTAAAATCGAATCGTATTCCGAGGTGTCTAATTGGCGCTCCGCACCTTCCGGATAATTTTTAAACCAAAGTTTTTCCATTGCCGTTCCTTAGGGAAGTTTGGTTa                                                                                                                         <  1:381062/164‑1 (MQ=255)                                                                                                                                                                                                   gCCCATATTAATATAAGCGGCACGATCGGGATGTTCGCGCACCGCTTTTTCAAACATATCTAAAATCGAATCGTATTCCGAGGTGTCTAATTGGCGCTCCGCACCTTCCGGATAATTTTTAAACCAAAGTTTTTCCATTGCCGTTCCTTAGGGAAGTTTGGTTATTGTTAATc                                                                                                                <  1:589375/173‑1 (MQ=255)                                                                                                                                                                                                       atatTAATATAAGCGGCACGATCGGGATGTTCGCGCACCGCTTTTTCAAACATATCTAAAATCGAATCGTATTCCGAGGTGTCTAATTGGCGCTCCGCACCTTCCGGATAATTTTTAAACCAAAGTTTTTCCATTGCCGTTCCTTAGGGAAGTTTGGTTATTGTTAATCGGGTTAATTAATATAAATTATAGCGCACGCGCGGTTCAGCCCAAAAGAAGCGGGACGAAAATCCCCAGCCGTAAGGGTaaaa                              >  1:254370/1‑251 (MQ=255)                                                                                                                                                                                                        tatTAATATAAGCGGCACGATCGGGATGTTCGCGCACCGCTTTTTCAAACATATCTAAaat                                                                                                                                                                                                                           <  1:211431/61‑1 (MQ=255)                                                                                                                                                                                                        tatTAATATAAGCGGCACGATCGGGATGTTCGCGCACCGCTTTTTCAAACATATCTAAAATCGAATCGTATTCCGAGGTGTCTAATTGGCGCTCCGCACCTTCCGGATAATTTTTAAACCAAAGTTTTTCCATTGCCGTTCCTTAGGGa                                                                                                                                   >  1:307255/1‑149 (MQ=255)                                                                                                                                                                                                        tatTAATATAAGCGGCACGATCGGGATGTTCGCGCACCGCTTTTTCAAACATATCTAAAATCGAATCGTATTCCGAGGTGTCTAATTGGCGCTCCGCACCTTCCGGATAATTTTTAAACCAAAGTTTTTCCATTGCCGTTCCTTAGGGAAGTTTGGTTATTGTTAATCGGGTTAATTAATATAAATTATAg                                                                                         <  1:762461/191‑1 (MQ=255)                                                                                                                                                                                                        tatTAATATAAGCGGCACGATCGGGATGTTCGCGCACCGCTTTTTCAAACATATCTAAAATCGAATCGTATTCCGAGGTGTCTAATTGGCGCTCCGCACCTTCCGGATAATTTTTAAACCAAAGTTTTTCCATTGCCGTTCCTTAGGGAAGTTTGGTTATTGTTAATCGGGTTAATTAATATAAATTATAGCGCACGCGCGGTTCAGCCCAAAAGAAGCGGGACGAAAATcc                                                >  1:368423/1‑232 (MQ=255)                                                                                                                                                                                                        tatTAATATAAGCGGCACGATCGGGATGTTCGCGCACCGCTTTTTCAAACATATCTAAAATCGAATCGTATTCCGAGGTGTCTAATTGGCGCTCCGCACCTTCCGGATAATTTTTAAACCAAAGTTTTTCCATTGCCGTTCCTTAGGGAAGTTTGGTTATTGTTAATCGGGTTAATTAATATAAATTATAGCGCACGCGCGGTTCAGCCCAAAAGAAGCGGGACGAAAATCCCCAGCCGTAAGGGTAAAAc                             <  1:374514/251‑1 (MQ=255)                                                                                                                                                                                                        tatTAATATAAGCGGCACGATCGGGATGTTCGCGCACCGCTTTTTCAAACATATCTAAAATCGAATCGTATTCCGAGGTGTCTAATTGGCGCTCCGCACCTTCCGGATAATTTTTAAACCAAAGTTTTTCCATTGCCGTTCCTTAGGGAAGTTTGGTTATTGTTAATCGGGTTAATTAATATAAATTATAGCGCACGCGCGGTTCAGCCCAAAAGAAGCGGGACGAAAATCCCCAGCCGTAAGGGTAAAAc                             <  1:293141/251‑1 (MQ=255)                                                                                                                                                                                                           tAATATAAGCGGCACGATCGGGATGTTCGCGCACCGCTTTTTCAAACATATCTAAAATCGAATCGTATTCCGAGGTGTCTAATTGGCGCTCCGCACCTTCCGGATAATTTTTAAACCAAAGTTTTTCCATTGCCGTTCCTTAGGGAAGTTTGGTTATTGTTAATCGGGTTAATTAATATAAATTATAGCGCACGCGCGGTTc                                                                           <  1:580628/202‑1 (MQ=255)                                                                                                                                                                                                             atatAAGCGGCACGATCGGGATGTTCGCGCACCGCTTTTTCAAACATATCTAAAATCGAATCGTATTCCGAGGTGTCTAATTGGCGCTCCGCACCTTCCGGATAATTTTTAAACCAAAGTTTTTCCATTGCCGTTCCTTAGGGAAGTTTGGTTATTGTTAATCGGGTTAATTAATATAAATTATAGCGCACGCGCGGTTCAGCCCAAAAGAAGCGGGACGAAAATCCCCAGCCGTAAGGGTAAAACGGTg                         >  1:779077/1‑250 (MQ=255)                                                                                                                                                                                                                    cGGCACGATCGGGATGTTCGCGCACCGCTTTTTCAAACATATCTAAAATCGAATCGTATTCCGAGGTGTCTAATTGGCGCTCCGCACCTTCCGGATAATTTTTAAACCAAAGTTTTTCCATTGCCGTTCCTTAGGGAAGTTTGGTTATTGTTAATCGGGTTAATTAATATAAATTATAGCGCACGCGCGGTTCAGCCCAAAAGAAGCGGGACGAAAATCCCCAGCCGTAAGGGTAAAACGGTGCGTAGTCa                 <  1:551311/251‑1 (MQ=255)                                                                                                                                                                                                                      gCACGATCGGGATGTTCGCGCACCGCTTTTTCAAACATATCTAAAATCGAATCGTATTCCGAGGTGTCTAATTGGCGCTCTGCACCTTCCGGATAATTTTTAAACCAAAGTTTTTCCATTGCCGTTCCTTAGGGAAGTTTGGTTATTGTTAATCGGGTTAATTAATATAAAtt                                                                                             <  1:545082/173‑1 (MQ=255)                                                                                                                                                                                                                         cGATCGGGATGTTCGCGCACCGCTTTTTCAAACATATCTAAAATCGAATCGTATTCCGAGGTGTCTAATTGGCGCTCCGCACCTTCCGGATAATTTTTAAACCAAAGTTTTTCCATTGCCGTTCCTTAGGGAAGTTTGGTTATTGTTAATCGGGTTAATTAATATAAATTATAGCGCACGCGCGGTTCAGCCCAAAAGAAGCGGGACGAAAATCCCCAGCCGTAAGGGTAAAACGGTGCGTAGTCATAAAt            <  1:544169/251‑1 (MQ=255)                                                                                                                                                                                                                           aTCGGGATGTTCGCGCACCGCTTTTTCAAACATATCTAAAATCGAATCGTATTCCGAGGTGTCTAATTGGCGCTCCGCACCTTCCGGATAATTTTTAAACCAAAGTTTTTCCATTGCCGTTCCTTAGGGAAGTTTGGTTATTGTTAATCGGGttaatta                                                                                                      >  1:807398/1‑159 (MQ=255)                                                                                                                                                                                                                           aTCGGGATGTTCGCGCACCGCTTTTTCAAACATATCTAAAATCGAATCGTATTCCGAGGTGTCTAATTGGCGCTCCGCACCTTCCGGATAATTTTTAAACCAAAGTTTTTCCATTGCCGTTCCTTAGGGAAGTTTGGTTATTGTTAATCGGGTTAATTAATATAAATTATAGCGCACGCGCGGTTCAGCCCAAAAGAAGCGGGACGAAAATCCCCAGCCGTAAGGGTAAAACGGTGCGTAGTCATAAATgt          >  1:755904/1‑251 (MQ=255)                                                                                                                                                                                                                                   gTTCGCGCACCGCTTTTTCAAACATATCTAAAATCGAATCGTATTCCGAGGTGTCTAATTGGCGCTCCGCACCTTc                                                                                                                                                                                 <  1:279686/76‑1 (MQ=255)                                                                                                                                                                                                                                   gTTCGCGCACCGCTTTTTCAAACATATCTAAAATCGAATCGTATTCCGAGGTGTCTAATTGGCGCTCCGCACCTTCCGGATAATTTTTAAACCAAAGTTTTTCCATTGCCGTTCCTTAGGGAAGTTTGGTTATTGTTAATCGGGTTAATTAATATAAATTATAGCGCACGCGCGGTTCAGCCCAAAAGAAGCGGGACGAAAATCCCCAGCCGTAAGGGTAAAACGGTGCGTAGTCATAAATGTCGTCAggg  >  1:356139/1‑251 (MQ=255)                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                       CGCGGCGTATAAAGCGGGTTCACATTGACCACCACCAACCCAGCACGCAACACCCCGAACAACGCAATGGGATATTGCAGCAAATTCGGCATCATTAATGCCACGCGATCGCCGCGCTCCAATTTCAATTCATTTTGCAAATAGGCAGCGAAAGCACGGCTACGTTCTTCCAATTTGCGAAACGTCAACACCTTGCCCATATTAATATAAGCGGCACGATCGGGATGTTCTCGCACCGCTTTTTCAAACATATCTAAAATCGAATCGTATTCCGAGGTGTCTAATTGGCGCTCCGCACCTTCCGGATAATTTTTAAACCAAAGTTTTTCCATTGCCGTTCCTTAGGGAAGTTTGGTTATTGTTAATCGGGTTAATTAATATAAATTATAGCGCACGCGCGGTTCAGCCCAAAAGAAGCGGGACGAAAATCCCCAGCCGTAAGGGTAAAACGGTGCGTAGTCATAAATGTCGTCAGGG  >  1/1809458‑1809934

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑