Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,808,419	A→C	intergenic (+13492/‑125912)	unknown → / → unknown	tRNA‑Leu‑CAA/tRNA‑Asp‑GTC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,808,419	0	A	C	100.0%	217.3 / NA	78	intergenic (+13492/‑125912)	unknown/unknown	tRNA‑Leu‑CAA/tRNA‑Asp‑GTC
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (34/44);  total (34/44)

CGGAACTCAATATCTTTCGGTATTTTGTAACCCGTTAGGTGTTCGCGGCAATGCGTACGCAGTTCATCGCGGGTCAGGCTTTCGTCTTTTTTCACCACAAAAAGCTTAATGGTTTCGCCGGAAACCTCATGAGGCACGCCGATGGCAACCACTTCGGAAACCTTGTTATTCAACATCACCACATCTTCGATTTCATTTGGATACACATTAAAACCGGATACCAGAATCATATCATTTTTGCGATCCACAATGCGTAAACTGTAATCCTCGTCCATAACCACAATGTCGCCGGTCGCCATCCAGCCGTCTTTAATCACTTCCGCAGTGGCTTCCGGGCGCTGCCAATAACCTTGCATAACCTGCTCGCCTTTCACCCATAACTCGCCCGCTTCGCCGATAGCCGCTTCCGTGTCATCTTCTTTAATAATTTTAATGTCCGTATTCGGCACCGGCACACCGATACTGCCGTTATGTTTCAC  >  1/1808186‑1808664                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                                      cgGAACTCAATATCTTTCGGTATTTTGTAACCCGTTAGGTGTTCGCGGCAATGCGTACGCAGTTCATCGCGGGTCAGGCTTTCGTCTTTTTTCACCACAAAAAGCTTAATGGTTTCGCCGGAAACCTCATGAGGCACGCCGATGGCAACCACTTCGGAAACCTTGTTATTCAACATCACCACATCTTCGATTTCATTTGGATACACATTAAAACCGGATACCAGAATCATATCCTTTTTGCGATCCACaa                                                                                                                                                                                                                                       <  1:661478/250‑1 (MQ=255) cgGAACTCAATATCTTTCGGTATTTTGTAA‑CCGTTAGGTGTTCGCGGCAATGCGTACGCAGTTCATCGCGGGTCAGGCTTTCGTCTTTTTTCACCACAAAAAGCTTAATGGTTTCGCCGGAAACCTCATGAGGCACGCCGATGGCAACCACTTCGGAAACCTTGTTATTCAACATCACCACATCTTCGATTTCATTTGGATACACATTAAAACCGGATACCAGAATCATATCCTTTTTGCGATCCACAATg                                                                                                                                                                                                                                     <  1:854369/251‑1 (MQ=255)     aCTCAATATCTTTCGGTATTTTGTAACCCGTTAGGTGTTCGCGGCAATGCGTACGCAGTTCATCGCGGGTCAGGCTTTCGTCTTTTTTCACCACAAAAAGCTTAATGGTTTCGCCGGAAACCTCATGAGGCACGCCGATGGCAACCACTTCGGAAACCTTGTTATTCAACATCACCACATCTTCGATTTCATTTGGATACACATTAAAACCGGATACCAGAATCATATCCTTTTTGCGATCCACaa                                                                                                                                                                                                                                       >  1:334829/1‑246 (MQ=255)           tatCTTTCGGTATTTTGTAACCCGTTAGGTGTTCGCGGCAATGCGTACGCAGTTCATCGCGGGTCAGGCTTTCGTCTTTTTTCACCACAAAAAGCTTAATGGTTTCGCCGGAAACCTCATGAGGCACGCCGATGGCAACCACTTCGGAAACCTTGTTATTCAACATCACCACATCTTCGATTTCATTTGGATACACATTAAAACCGGATACCAGAATCATATCCTTTTTGCGATCCACAATGCGTAAACTg                                                                                                                                                                                                                            <  1:846934/251‑1 (MQ=255)                 tCGGTATTTTGTAACCCGTTAGGTGTTCGCGGCAATGCGTACGCAGTTCATCGCGGGTCAGGCTTTCGTCTTTTTTCACCACAAAAAGCTTAATGGTTTCGCCGGAAACCTCATGAGGCACGCCGATGGCAACCACTTCGGAAACCTTGTTATTCAACATCACCACATCTTCGATTTCATTTGGATACACATTAAAACCGGATACCAGAATCATATCCTTTTTGCGATCCACAATGCGTAAACTGTAATcc                                                                                                                                                                                                                      <  1:770297/251‑1 (MQ=255)                      aTTTTGTAACCCGTTAGGTGTTCGCGGCAATGCGTACGCAGTTCATCGCGGGTCAGGCTTTCGTCTTTTTTCACCACAAAAAGCTTAATGGTTTCGCCGGAAACCTCATGAGGCACGCCGATGGCAACCACTTCGGAAACCTTGTTATTCAACATCACCACATCTTCGATTTCATTTGGATACACATTAAAACCGGATACCAGAATCATATCCTTTTTGCGATCcac                                                                                                                                                                                                                                         <  1:321574/227‑1 (MQ=255)                       ttttGTAACCCGTTAGGTGTTCGCGGCAATGCGTACGCAGTTCATCGCGGGTCAGGCTTTCGTCTTTTTTCACCACAAAAAGCTTAATGGTTTCGCCGGAAACCTCATGAGGCACGCCGATGGCAACCACTTCGGAAACCTTGTTATTCAACATCACCACATCTTCGATTTCATTTGGATACACATTAAAACCGGATACCAGAATCATATCCTTTTTGCGATCCACAATGCGTAAACTGTAATCCTCGTcc                                                                                                                                                                                                                <  1:70116/251‑1 (MQ=255)                        tttGTAACCCGTTAGGTGTTCGCGGCAATGCGTACGCAGTTCATCGCGGGTCAGGCTTTCGTCTTTTTTCACCACAAAAAGCTTAATGGTTTCGCCGGAAACCTCATGAGGCACGCCGATGGCAACCACTTCGGAAACCTTGTTATTCAACATCACCACATCTTCGATTTCATTTGGATACACATTAAAACCGGATACCAGAATCATATCCTTTTTGCGATCCACAATGCGTAAACTGTAATCCTCGTCCa                                                                                                                                                                                                               >  1:738891/1‑251 (MQ=255)                        tttGTAACCCGTTAGGTGTTCGCGGCAATGCGTACGCAGTTCATCGCGGGTCAGGCTTTCGTCTTTTTTCACCACAAAAAGCTTAATGGTTTCGCCGGAAACCTCATGAGGCACGCCGATGGCAACCACTTCGGAAACCTTGTTATTCAACATCACCACATCTTCGATTTCATTTGGATACACATTAAAACCGGATACCAGAATCATATCCTTTTTGCGATCCACAATGCGTAAACTGTAATCCTCGTCCa                                                                                                                                                                                                               >  1:391627/1‑251 (MQ=255)                        tttGTAACCCGTTAGGTGTTCGCGGCAATGCGTACGCAGTTCATCGCGGGTCAGGCTTTCGTCTTTTTTCACCACAAAAAGCTTAATGGTTTCGCCGGAAACCTCATGAGGCACGCCGATGGCAACCACTTCGGAAACCTTGTTATTCAACATCACCACATCTTCGATTTCATTTGGATACACATTAAAACCGGATACCAGAATCATATCCTTTTTGCGATCCACAATGCGTAAACTGTAATCCTCGTCCa                                                                                                                                                                                                               >  1:438635/1‑251 (MQ=255)                               cccGTTAGGTGTTCGCGGCAATGCGTACGCAGTTCATCGCGGGTCAGGCTTTCGTCTTTTTTCACCACAAAAAGCTTAATGGTTTCGCCGGAAACCTCATGAGGCACGCCGATGGCAACCACTTCGGAAACCTTGTTATTCAACATCACCACATCTTCGATTTCATTTGGATACACATTAAAACCGGATACCAGAATCATATCCTTTTTGCGATCCACAATGCGTAAACTg                                                                                                                                                                                                                            >  1:122022/1‑231 (MQ=255)                                 cGTTAGGTGTTCGCGGCAATGCGTACGCAGTTCATCGCGGGTCAGGCTTTCGTCTTTTTTCACCACAAAAAGCTTAATGGTTTCGCCGGAAACCTCATGAGGCACGCCGATGGCAACCACTTCGGAAACCTTGTTATTCAACATCACCACATCTTCGATTTCATTTGGATACACATTAAAACCGGATACCAGAATCATATCCTTTTTGCGATCCACAATGCGTAAACTg                                                                                                                                                                                                                            >  1:667526/1‑229 (MQ=255)                                 cGTTAGGTGTTCGCGGCAATGCGTACGCAGTTCATCGCGGGTCAGGCTTTCGTCTTTTTTCACCACAAAAAGCTTAATGGTTTCGCCGGAAACCTCATGAGGCACGCCGATGGCAACCACTTCGGAAACCTTGTTATTCAACATCACCACATCTTCGATTTCATTTGGATACACATTAAAACCGGATACCAGAATCATATCCTTTTTGCGATCCACAATGCGTAAACTGTAATCCTCGTCCATAACCACaa                                                                                                                                                                                                      >  1:788682/1‑251 (MQ=255)                                 cGTTAGGTGTTCGCGGCAATGCGTACGCAGTTCATCGCGGGTCAGGCTTTCGTCTTTTTTCACCACAAAAAGCTTAATGGTTTCGCCGGAAACCTCATGAGGCACGCCGATGGCAACCACTTCGGAAACCTTGTTATTCAACATCACCACATCTTCGATTTCATTTGGATACACATTAAAACCGGATACCAGAATCATATCCTTTTTGCGATCCACAATGCGTAAACTGTAATCCTCGTCCATAACCACaa                                                                                                                                                                                                      >  1:900007/1‑251 (MQ=255)                                       gtgtTCGCGGCAATGCGTACGCAGTTCATCGCGGGTCAGGCTTTCGTCTTTTTTCACCACAAAAAGCTTAATGGTTTCGCCGGAAACCTCATGAGGCACGCCGATGGCAACCACTTCGGAAACCTTGTTATTCAACATCACCACATCTTCGATTTCATTTGGATACACATTAAAACCGGATACCAGAATCATATCCTTTTTGCGATCCACAATGCGTAAACTGTAATCCTCGTCCATAACCACAATGt                                                                                                                                                                                                   >  1:861423/1‑248 (MQ=255)                                           tCGCGGCAATGCGTACGCAGTTCATCGCGGGTCAGGCTTTCGTCTTTTTTCACCACAAAAAGCTTAATGGTTTCGCCGGAAACCTCATGAGGCACGCCGATGGCAACCACTTCGGAAACCTTGTTATTCAACATCACCACATCTTCGATTTCATTTGGATACACATTAAAACCGGATACCAGAATCATATCCTTTTTGCGATCCACAATGCGTAAACTGTAATCCTCGTCCATAACCACAATGTCGCCGGt                                                                                                                                                                                            >  1:123376/1‑251 (MQ=255)                                              cgGCAATGCGTACGCAGTTCATCGCGGGTCAGGCTTTCGTCTTTTTTCACCACAAAAAGCTTAATGGTTTCGCCGGAAACCTCATGAGGCACGCCGATGGCAACCACTTCGGAAACCTTGTTATTCAACATCACCACATCTTCGATTTCATTTGGATACACATTAAAACCGGATACCAGAATCATATCCTTTTTGCGATCCACAATGCGTAAACTGTAATCCTCGTCCATAACCACAATGTCGCCGGTCg                                                                                                                                                                                          >  1:262063/1‑250 (MQ=255)                                               ggCAATGCGTACGCAGTTCATCGCGGGTCAGGCTTTCGTCTTTTTTCACCACAAAAAGCTTAATGGTTTCGCCGGAAACCTCATGAGGCACGCCGATGGCAACCACTTCGGAAACCTTGTTATTCAACATCACCACATCTTCGATTTCATTTGGATACACATTAAAACCGGATACCAGAATCATATCCTTTTTGCGATCCACAATGCGTAAACTGTAATCCTCGTCCATAACCACAATGTCGCCGGTCGc                                                                                                                                                                                         >  1:37330/1‑250 (MQ=255)                                                    tGCGTACGCAGTTCATCGCGGGTCAGGCTTTCGTCTTTTTTCACCACAAAAAGCTTAATGGTTTCGCCGGAAACCTCATGAGGCACGCCGATGGCAACCACTTCGGAAACCTTGTTATTCAACATCACCACATCTTCGATTTCATTTGGATACACATTAAAACCGGATACCAGAATCATATCCTTTTTGCGATCCACAATGCGTAAAc                                                                                                                                                                                                                              <  1:163336/208‑1 (MQ=255)                                                    tGCGTACGCAGTTCATCGCGGGTCAGGCTTTCGTCTTTTTTCACCACAAAAAGCTTAATGGTTTCGCCGGAAACCTCATGAGGCACGCCGATGGCAACCACTTCGGAAACCTTGTTATTCAACATCACCACATCTTCGATTTCATTTGGATACACATTAAAACCGGATACCAGAATCATATCCTTTTTGCGATCCACAATGCGTAAACTGTAATcc                                                                                                                                                                                                                      <  1:43008/216‑1 (MQ=255)                                                      cGTACGCAGTTCATCGCGGGTCAGGCTTTCGTCTTTTTTCACCACAAAAAGCTTAATGGTTTCGCCGGAAACCTCATGAGGCACGCCGATGGCAACCACTTCGGAAACCTTGTTATTCAACATCACCACATCTTCGATTTCATTTGGATACACATTAAAACCGGATACCAGAATCATATCCTTTTTGCGATCCACAATGCGTAAACTGTAATCCTCGTCCATAACCACAATGTCGCCGGTCGCCATCCAg                                                                                                                                                                                  >  1:9102/1‑250 (MQ=255)                                                      cGTACGCAGTTCATCGCGGGTCAGGCTTTCGTCTTTTTTCACCACAAAAAGCTTAATGGTTTCGCCGGAAACCTCATGAGGCACGCCGATGGCAACCACTTCGGAAACCTTGTTATTCAACATCACCACATCTTCGATTTCATTTGGATACACATTAAAACCGGATACCAGAATCATATCCTTTTTGCGATCCACAATGCGTAAACTGTAATCCTCGTCCATAACCACAATGTCGCCGGTCGCCATCCAGc                                                                                                                                                                                 <  1:656925/251‑1 (MQ=255)                                                              gTTCATCGCGGGTCAGGCTTTCGTCTTTTTTCACCACAAAAAGCTTAATGGTTTCGCCGGAAACCTCATGAGGCACGCCGATGGCAACCACTTCGGAAACCTTGTTATTCAACATCACCACATCTTCGATTTCATTTGGATACACATTAAAACCGGATACCAGAATCATATCCTTTTTGCGATCCACAATGCGTAAACTGTAATCCTCGTCCATAACcaca                                                                                                                                                                                                       <  1:332695/221‑1 (MQ=255)                                                              gTTCATCGCGGGTCAGGCTTTCGTCTTTTTTCACCACAAAAAGCTTAATGGTTTCGCCGGAAACCTCATGAGGCACGCCGATGGCAACCACTTCGGAAACCTTGTTATTCAACATCACCACATCTTCGATTTCATTTGGATACACATTAAAACCGGATACCAGAATCATATCCTTTTTGCGATCCACAATGCGTAAACTGTAATCCTCGTCCATAACCACAATGTCGCCGGTCGCCATCCAGCCGTCTTTa                                                                                                                                                                         >  1:18812/1‑251 (MQ=255)                                                              gTTCATCGCGGGTCAGGCTTTCGTCTTTTTTCACCACAAAAAGCTTAATGGTTTCGCCGGAAACCTCATGAGGCACGCCGATGGCAACCACTTCGGAAACCTTGTTATTCAACATCACCACATCTTCGATTTCATTTGGATACACATTAAAACCGGATACCAGAATCATATCCTTTTTGCGATCCACAATGCGTAAACTGTAATCCTCGTCCATAACCACAATGTCGCCGGTCGCCATCCAGCCGTCTTTa                                                                                                                                                                         >  1:578005/1‑251 (MQ=255)                                                                 cATCGCGGGTCAGGCTTTCGTCTTTTTTCACCACAAAAAGCTTAATGGTTTCGCCGGAAACCTCATGAGGCACGCCGATGGCAACCACTTCGGAAACCTTGTTATTCAACATCACCACATCTTCGATTTCATTTGGATACACATTAAAACCGGATACCAGAATCATATCCTTTTTGCGATCCACAATGCGTAAACTGTAATCCTCGTCCATAACCACAATGTCGCCGGTCGCCATCCAGCCGTCTTTAATc                                                                                                                                                                      <  1:263325/251‑1 (MQ=255)                                                                 cATCGCGGGTCAGGCTTTCGTCTTTTTTCACCACAAAAAGCTTAATGGTTTCGCCGGAAACCTCATGAGGCACGCCGATGGCAACCACTTCGGAAACCTTGTTATTCAACATCACCACATCTTCGATTTCATTTGGATACACATTAAAACCGGATACCAGAATCATATCCTTTTTGCGATCCACAATGCGTAAACTGTAATCCTCGTCCATAACCACAATGTCGCCGGTCGCCATCCAGCCGTCTTTAATc                                                                                                                                                                      <  1:757655/251‑1 (MQ=255)                                                                 cATCGCGGGTCAGGCTTTCGTCTTTTTTCACCACAAAAAGCTTAATGGTTTCGCCGGAAACCTCATGAGGCACGCCGATGGCAACCACTTCGGAAACCTTGTTATTCAACATCACCACATCTTCGATTTCATTTGGATACACATTAAAACCGGATACCAGAATCATATCCTTTTTGCGATCCACAATGCGTAAACTGTAATCCTCGTCCATAACCACAATGTCGCCGGTCGCCATCCAGCCGTCTTTAATc                                                                                                                                                                      <  1:135196/251‑1 (MQ=255)                                                                 cATCGCGGGTCAGGCTTTCGTCTTTTTTCACCACAAAAAGCTTAATGGTTTCGCCGGAAACCTCATGAGGCACGCCGATGGCAACCACTTCGGAAACCTTGTTATTCAACATCACCACATCTTCGATTTCATTTGGATACACATTAAAACCGGATACCAGAATCATATCCTTTTTGCGATCCACAATGCGTAAACTGTAATCCTCGTCCATAACCACAATGTCGCCGGTCGCCATCCAGCCGTCTTTAATc                                                                                                                                                                      <  1:161699/251‑1 (MQ=255)                                                                              gCTTTCGTCTTTTTTCACCACAAAAAGCTTAATGGTTTCGCCGGAAACCTCATGAGGCACGCCGATGGCAACCACTTCGGAAACCTTGTTATTCAACATCACCACATCTTCGATTTCATTTGGATACACATTAAAACCGGATACCAGAATCATATCCTTTTTGCGATCCACAATGCGTAAACTGTAATCCTCGTCCATAACCACAATGTCGCCGGTCGCCATCCAGCCGTCTTTAATCACTTCCGCAg                                                                                                                                                            <  1:716605/248‑1 (MQ=255)                                                                              gCTTTCGTCTTTTTTCACCACAAAAAGCTTAATGGTTTCGCCGGAAACCTCATGAGGCACGCCGATGGCAACCACTTCGGAAACCTTGTTATTCAACATCACCACATCTTCGATTTCATTTGGATACACATTAAAACCGGATACCAGAATCATATCCTTTTTGCGATCCACAATGCGTAAACTGTAATCCTCGTCCATAACCACAATGTCGCCGGTCGCCATCCAGCCGTCTTTAATCACTTCCGCAg                                                                                                                                                            <  1:589569/248‑1 (MQ=255)                                                                              gCTTTCGTCTTTTTTCACCACAAAAAGCTTAATGGTTTCGCCGGAAACCTCATGAGGCACGCCGATGGCAACCACTTCGGAAACCTTGTTATTCAACATCACCACATCTTCGATTTCATTTGGATACACATTAAAACCGGATACCAGAATCATATCCTTTTTGCGATCCACAATGCGTAAACTGTAATCCTCGTCCATAACCACAATGTCGCCGGTCGCCATCCAGCCGTCTTTAATCACTTCCGCAg                                                                                                                                                            <  1:398872/248‑1 (MQ=255)                                                                                     tCTTTTTTCACCACAAAAAGCTTAATGGTTTCGCCGGAAACCTCATGAGGCACGCCGATGGCAACCACTTCGGAAACCTTGTTATTCAACATCACCACATCTTCGATTTCATTTGGATACACATTAAAACCGGATACCAGAATCATATCCTTTTTGCGATCCACAATGCGTAAACTGTAATCCTCGTCCATAACCACAATGTCGCCGGTCGCCATCCAGCCGTCTTTAATCACTTCCGCAGTGGCTTCCgg                                                                                                                                                  <  1:68580/251‑1 (MQ=255)                                                                                                 acaAAAAGCTTAATGGTTTCGCCGGAAACCTCATGAGGCACGCCGATGGCAACCACTTCGGAAACCTTGTTATTCAACATCACCACATCTTCGATTTCATTTGGATACACATTAAAACCGGATACCAGAATCATATCCTTTTTGCGATCcac                                                                                                                                                                                                                                         >  1:54570/1‑152 (MQ=255)                                                                                                  caAAAAGCTTAATGGTTTCGCCGGAAACCTCATGAGGCACGCCGATGGCAACCACTTCGGAAACCTTGTTATTCAACATCACCACATCTTCGATTTCATTTGGATACACATTAAAACCGGATACCAGAATCATATCCTTTTTGCGATCCACAATGCGTAAACTGTAATCCTCGTCCATAAc                                                                                                                                                                                                           >  1:83538/1‑181 (MQ=255)                                                                                                   aaaaaGCTTAATGGTTTCGCCGGAAACCTCATGAGGCACGCCGATGGCAACCACTTCGGAAACCTTGTTATTCAACATCACCACATCTTCGATTTCATTTGGATACACATTAAAACCGGATACCAGAATCATATCCTTTTTGCGATCCACAATGCGTAAACTGTAATCCTCGTCCATAACCACAATGTCGCCGGTCGCCATCCAGCCGTCTTTAATCACTTCCGCAGTGGCTTCCGGGCGCTGCCAATAAc                                                                                                                                    <  1:492991/251‑1 (MQ=255)                                                                                                   aaaaaGCTTAATGGTTTCGCCGGAAACCTCATGAGGCACGCCGATGGCAACCACTTCGGAAACCTTGTTATTCAACATCACCACATCTTCGATTTCATTTGGATACACATTAAAACCGGATACCAGAATCATATCCTTTATGCGATCCACAATGCGTAAACTGTAATCCTCGTCCATAACCCCAATGTCGCCGGTCGCCATCCAGCCGTCTTTAATCACTTCCGCAGTGGCTTCCGGGCGCTGCCAATAAc                                                                                                                                    <  1:549555/251‑1 (MQ=255)                                                                                                      aaGCTTAATGGTTTCGCCGGAAACCTCATGAGGCACGCCGATGGCAACCACTTCGGAAACCTTGTTATTCAACATCACCACATCTTCGATTTCATTTGGATACACATTAAAACCGGATACCAGAATCATATCCTTTTTGCGATCCACAATGCGTAAACTGTAATCCTCGTCCATAACCACAATGTCGCCGGTCGCCATCCAGCCGTCTTTAATCACTTCCGCAGTGGCTTCCGGGCGCTGCCAATAACCtt                                                                                                                                 >  1:123501/1‑251 (MQ=255)                                                                                                        gCTTAATGGTTTCGCCGGAAACCTCATGAGGCACGCCGATGGCAACCACTTCGGAAACCTTGTTATTCAACATCACCACATCTTCGATTTCATTTGGATACACATTAAAACCGGATACCAGAATCATATCCTTTTTGCGATCCACAATGCGTAAACTGTAATcc                                                                                                                                                                                                                      >  1:632345/1‑164 (MQ=255)                                                                                                        gCTTAATGGTTTCGCCGGAAACCTCATGAGGCACGCCGATGGCAACCACTTCGGAAACCTTGTTATTCAACATCACCACATCTTCGATTTCATTTGGATACACATTAAAACCGGATACCAGAATCATATCCTTTTTGCGATCCACAATGCGTAAACTGTAATCCTCGTCCATAACCACAATGTCGCCGGTCGCCATCCAGCCGTCTTTAATCACTTCCGCAg                                                                                                                                                            <  1:817630/222‑1 (MQ=255)                                                                                                              tGGTTTCGCCGGAAACCTCATGAGGCACGCCGATGGCAACCACTTCGGAAACCTTGTTATTCAACATCACCACATCTTCGATTTCATTTGGATACACATTAAAACCGGATACCAGAATCATATCCTTTTTGCGATCCACAATGCGTAAACTGTAATCCTCGTCCATAACCACAATGTCGCCGGTCGCCATCCAGCCGTCTTTAATCACTTCCGCAGTGGCTTCCGGGCGCTGCCAATAACCTTGCATAAcc                                                                                                                         <  1:267305/251‑1 (MQ=255)                                                                                                                             ccTCATGAGGCACGCCGATGGCAACCACTTCGGAAACCTTGTTATTCAACATCACCACATCTTCGATTTCATTTGGATACACATTAAAACCGGATACCAGAATCATATCCTTTTTGCGATCCACAATGCGTAAACTGTAATCCTCGTCCATAACCACAATGTCGCCGGTCGCCATCCAGCCGTCTTTAATCACTTCCGCAGTGGCTTCCGGGCGCTGCCAATAACCTTGCATAACCTGCTCGCCTTTCAc                                                                                                           >  1:538444/1‑250 (MQ=255)                                                                                                                                cATGAGGCACGCCGATGGCAACCACTTCGGAAACCTTGTTATTCAACATCACCACATCTTCGATTTCATTTGGATACACATTAAAACCGGATACCAGAATCATATCCTTTTTGCGATCCACAATGCGTAAACTGTAATCCTCGTCCATAACCACAATGTCGCCGGTCGCCATCCAGCCGTCTTTAATCACTTCCGCAGTGGCTTCCGGGCGCTGCCAATAACCTTGCATAACCTGCTCGCCTTTCACCCAt                                                                                                       >  1:434870/1‑251 (MQ=255)                                                                                                                                      gCACGCCGATGGCAACCACTTCGGAAACCTTGTTATTCAACATCACCACATCTTCGATTTCATTTGGATACACATTAAAACCGGATACCAGAATCATATCCTTTTTGCGATCCACAATGCGTAAACTGTAATCCTCGTCCATAACCACAATGTCGCCGGTCGCCATCCAGCCGTCTTTAATCACTTCCGCAGTGGCTTCCGGGCGCTGCCAATAACCTTGCATAACCTGCTCGCCTTTCACCCATAACt                                                                                                   <  1:805971/249‑1 (MQ=255)                                                                                                                                      gCACGCCGATGGCAACCACTTCGGAAACCTTGTTATTCAACATCACCACATCTTCGATTTCATTTGGATACACATTAAAACCGGATACCAGAATCATATCCTTTTTGCGATCCACAATGCGTAAACTGTAATCCTCGTCCATAACCACAATGTCGCCGGTCGCCATCCAGCCGTCTTTAATCACTTCCGCAGTGGCTTCCGGGCGCTGCCAATAACCTTGCATAACCTGCTCGCCTTTCACCCATAACt                                                                                                   <  1:413940/249‑1 (MQ=255)                                                                                                                                             gATGGCAACCACTTCGGAAACCTTGTTATTCAACATCACCACATCTTCGATTTCATTTGGATACACATTAAAACCGGATACCAGAATCATATCCTTTTTGCGATCCACAATGCGTAAACTGTAATcc                                                                                                                                                                                                                      >  1:26965/1‑127 (MQ=255)                                                                                                                                                       aCTTCGGAAACCTTGTTATTCAACATCACCACATCTTCGATTTCATTTGGATACACATTAAAACCGGATACCAGAATCATATCCTTTTTGCGATCCACAATGCGTAAACTGTAATCCTCGTCCATAACCACAATGTCGCCGGTCGCCATCCAGCCGTCTTTAATCACTTCCGCAGTGGCTTCCGGGCGCTGCCAATAACCTTGCATAACCTGCTCGCCTTTCACCCATAACTCGCCCGCTTCGCCGATAGc                                                                                <  1:191024/251‑1 (MQ=255)                                                                                                                                                        cTTCGGAAACCTTGTTATTCAACATCACCACATCTTCGATTTCATTTGGATACACATTAAAACCGGATACCAGAATCATATCCTTTTTGCGATCCACAATGCGTAAACTGTAATCCTCGTCCATAACCACAATGTCGCCGGTCGCCATCCAGCCGTCTTTAATCACTTCCGCAGTGGCTTCCGGGCGCTGCCAATAACCTTGCATAACCTGCTCGCCTTTCAccc                                                                                                         >  1:889488/1‑225 (MQ=255)                                                                                                                                                         ttCGGAAACCTTGTTATTCAACATCACCACATCTTCGA‑TTCATTTGGATACACATTAAAACCGGATACCAGAATCATATCCTTTTTGCGATCCACAATGCGTAAACTGTAATCCTCGTCCATAACCACAATGTCGCCGGTCGCCATCCAGCCGTCTTTAATCACTTCCGCAGTGGCTTCCGGGCGCTGCCAATAACCTTGCATAACCTGCTCGCCTTTCACCCATAACTCGCCCGCTTCGCCGATAGCCGc                                                                             <  1:617244/251‑1 (MQ=255)                                                                                                                                                                aCCTTGTTATTCAACATCACCACATCTTCGATTTCATTTGGATACACATTAAAACCGGATACCAGAATCATATCCTTTTTGCGATCCACAATGCGTAAACTGTAATCCTCGTCCATAACCACAATGTCGCCGGTCGCCATCCAGCCGTCTTTAATCACTTCCGCAGTGGCTTCCGGGCGCTGCCAATAACCTTGCATAACCTGCTCGCCTTTCACCCATAACTCGCCCGCTTCGCCGATAGCCGCTTCCgt                                                                       >  1:22310/1‑251 (MQ=255)                                                                                                                                                                  cTTGTTATTCAACATCACCACATCTTCGATTTCATTTGGATACACATTAAAACCGGATACCAGAATCATATCCTTTTTGCGATCCACAATGCGTAAACTGTAATCCTCGTCCATAACCACAATGTCGCCGGTCGCCATCCAGCCGTCTTTAATCACTTCCGCAGTGGCTTCCGGGCGCTGCCAATAACCTTGCATAACCTGCTCGCCTTTCACCCATAACTCGCCCGCTTCGCCGATAGCCGCTTCCgtgt                                                                     <  1:719861/251‑1 (MQ=255)                                                                                                                                                                  cTTGTTATTCAACATCACCACATCTTCGATTTCATTTGGATACACATTAAAACCGGATACCAGAATCATATCCTTTTTGCGATCCACAATGCGTAAACTGTAATCCTCGTCCATAACCACAATGTCGCCGGTCGCCATCCAGCCGTCTTTAATCACTTCCGCAGTGGCTTCCGGGCGCTGCCAATAACCTTGCATAACCTGCTCGCCTTTCACCCATAACTCGCCCGCTTCGCCGATAGCCGCTTCCgtgt                                                                     <  1:472748/251‑1 (MQ=255)                                                                                                                                                                   ttGTTATTCAACATCACCACATCTTCGATTTCATTTGGATACACATTAAAACCGGATACCAGAATCATATCCTTTTTGCGATCCACAATGCGTAAACTGTAATCCTCGTCCATAACCACAATGTCGCCGGTCGCCATCCAGCCGTCTTTAATCACTTCCGCAGTGGCTTCCGGGCGCTGCCAATAACCTTGCATAACCTGCTCGCCTTTCACCCATAACTCGCCCGCTTCGCCGATAGCCGCTTCCgtgt                                                                     >  1:776695/1‑250 (MQ=255)                                                                                                                                                                      ttATTCAACATCACCACATCTTCGATTTCATTTGGATACACATTAAAACCGGATACCAGAATCATATCCTTTTTGCGATCCACAATGCGTAAACTGTAATcc                                                                                                                                                                                                                      <  1:895959/102‑1 (MQ=255)                                                                                                                                                                      ttATTCAACATCACCACATCTTCGATTTCATTTGGATACACATTAAAACCGGATACCAGAATCATATCCTTTTTGCGATCCACAATGCGTAAACTGTAATCCTCGTCCATAACCACAATGTCGCCGGTCGCCATCCAGCCGTCTTTAATCACTTCCGCAGTGGCTTCCGGGCGCTGCCAATAACCTTGCATAACCTGCTCGCCTTTCACCCATAACTCGCCCGCTTCGCCGATAGCCGCTTCCGTGTCAtc                                                                 <  1:315065/251‑1 (MQ=255)                                                                                                                                                                       tATTCAACATCACCACATCTTCGATTTCATTTGGATACACATTAAAACCGGATACCAGAATCATATCCTTTTTGCGATCCACAATGCGTAAACTGTAATCCTCGTCCATAACCACAATGTCGCCGGTCGCCATCCAGCCGTCTTTAATCACTTCCGCAGTGGCTTCCGGGCGCTGCCAATAACCTTGCATAACCTGCTCGCCTTTCACCCATAACTCGCCCGCTTCGCCGATAGCCGCTTCCGTGTCAtct                                                                <  1:385405/251‑1 (MQ=255)                                                                                                                                                                           cAACATCACCACATCTTCGATTTCATTTGGATACACATTAAAACCGGATACCAGAATCATATCCTTTTTGCGATCCACAATGCGTAAACTGTAATCCTCGTCCATAACCACAATGTCGCCGGTCGCCATCCAGCCGTCTTTAATCACTTCCGCAGTgg                                                                                                                                                         <  1:444907/158‑1 (MQ=255)                                                                                                                                                                                tCACCACATCTTCGATTTCATTTGGATACACATTAAAACCGGATACCAGAATCATATCCTTTTTGCGATCCACAATGCGTAAACTGTAATCCTCGTCCATAACCACAATGTCGCCGGTCGCCATCCAGCCGTCTTTAATCACTTCCGCAGTGGCTTCCGGGCGCTGCCAATAACCTTGCATAACCTGCTCGCCTTTCACCCATAACTCGCCCGCTTCGCCGATAGCCGCTTCCGTGTCATCTTCTTtaata                                                       >  1:114883/1‑251 (MQ=255)                                                                                                                                                                                tCACCACATCTTCGATTTCATTTGGATACACATTAAAACCGGATACCAGAATCATATCCTTTTTGCGATCCACAATGCGTAAACTGTAATCCTCGTCCATAACCACAATGTCGCCGGTCGCCATCCAGCCGTCTTTAATCACTTCCGCAGTGGCTTCCGGGCGCTGCCAATAACCTTGCATAACCTGCTCGCCTTTCACCCATAACTCGCCCGCTTCGCCGATAGCCGCTTCCGTGTCATCTTCTTtaat                                                        >  1:763060/1‑250 (MQ=255)                                                                                                                                                                                        tCTTCGATTTCATTTGGATACACATTAAAACCGGATACCAGAATCATATCCTTTTTGCGATCCACAATGCGTAAACTGTAATCCTCGTCCATAACCACAATGTCGCCGGTCGCCATCCAGCCGTCTTTAATCACTTCCGCAGTGGCTTCCGGGCGCTGCCAATAAc                                                                                                                                    >  1:346670/1‑166 (MQ=255)                                                                                                                                                                                              aTTTCATTTGGATACACATTAAAACCGGATACCAGAATCATATCCTTTTTGCGATCCACAATGCGTAAACTGTAATCCTCGTCCATAACCACAATGTCGCCGGTCGCCATCCAGCCGTCTTTAATCACTTCCGCAGTGGCTTCCGGGCGCTGCCAATAACCTTGCATAACCTGCTCGCCTTTCACCCATAACTCGCCCGCTTCGCCGATAGCCGCTTCCGTGTCATCTTCTTTAATAATTTTAATGTCCGt                                         >  1:276161/1‑251 (MQ=255)                                                                                                                                                                                                 tCATTTGGATACACATTAAAACCGGATACCAGAATCATATCCTTTTTGCGATCCACAATGCGTAAACTGTAATCCTCGTCCATAACCACAATGTCGCCGGTCGCCATCCAGc                                                                                                                                                                                 <  1:630404/112‑1 (MQ=255)                                                                                                                                                                                                  cATTTGGATACACATTAAAACCGGATACCAGAATCATATCCTTTTTGCGATCCACAATGCGTAAACTGTAATCCTCGTCCATAACCACAATGTCGCCGGTCGCCATCCAGCCGTCTTTAATCACTTCCGCAGTGGCTTCCGGGCGCTGCCAATAACCTTGCATAACCTGCTCGCCTTTCACCCATAACTCGCCCGCTTCGCCGATAGCCGCTTCCGTGTCATCTTCTTTAATAATTTTAATGTCCGTAtt                                      >  1:282023/1‑250 (MQ=255)                                                                                                                                                                                                    tttGGATACACATTAAAACCGGATACCAGAATCATATCCTTTTTGCGATCCACAATGCGTAAACTGTAATCCTCGTCCATAACCACAATGTCGCCGGTCGCCATCCAGCCGTCTTTAATCACTTCCGCAGTGGCTTCCGGGCGCTGCCAATAACCTTGCATAACCTGCTCGCCTTTCACCCATAACTCGCCCGCTTCGCCGATAGCCGCTTCCGTGTCATCTTCTTTAATAATTTTAATGTCCGTATTcgg                                   >  1:48396/1‑251 (MQ=255)                                                                                                                                                                                                       ggATACACATTAAAACCGGATACCAGAATCATATCCTTTTTGCGATCCACAATGCGTAAACTGTAATCCTCGTCCATAACCACAATGTCGCCGGTCGCCATCCAGCCGTCTTTAATCACTTCCGCAGTGGCTTCCGGGCGCTGCCAATAACCTTGCATAACCTGCTCGCCTTTCACCCATAACTCGCCCGCTTCGCCGATAGCCGCTTCCGTGTCATCTTCTTTAATAATTTTAATGTCCGTATTcggca                                 >  1:199905/1‑250 (MQ=255)                                                                                                                                                                                                            cacaTTAAAACCGGATACCAGAATCATATCCTTTTTGCGATCCACAATGCGTAAACTGTAATCCTCGTCCATAACCACAATGTCGCCGGTCGCCATCCAGCCGTCTTTAATCACTTCCGCAGTGGCTTCCGGGCGCTGCCAATAACCTTGCATAACCTGCTCGCCTTTCACCCATAACTCGCCCGCTTCGCCGATAGCCGCTTCCGTGTCATCTTCTTTAATAATTTTAATGTCCGTATTcggcaccggca                           <  1:805967/251‑1 (MQ=255)                                                                                                                                                                                                              caTTAAAACCGGATACCAGAATCATATCCTTTTTGCGATCCACAATGCGTAAACTGTAATCCTCGTCCATAACCACAATGTCGCCGGTCGCCATCCAGCCGTCTTTAATCACTTCCGCAGTGGCTTCCGGGCGCTGCCAATAACCTTGCATAAcc                                                                                                                         <  1:320426/155‑1 (MQ=255)                                                                                                                                                                                                              caTTAAAACCGGATACCAGAATCATATCCTTTTTGCGATCCACAATGCGTAAACTGTAATCCTCGTCCATAACCACAATGTCGCCGGTCGCCATCCAGCCGTCTTTAATCACTTCCGCAGTGGCTTCCGGGCGCTGCCAATAACCTTGCATAAcc                                                                                                                         <  1:789614/155‑1 (MQ=255)                                                                                                                                                                                                              caTTAAAACCGGATACCAGAATCATATCCTTTTTGCGATCCACAATGCGTAAACTGTAATCCTCGTCCATAACCACAATGTCGCCGGTCGCCATCCAGCCGTCTTTAATCACTTCCGCAGTGGCTTCCGGGCGCTGCCAATAACCTTGCATAACCTGCTCGCCTTTCACCCATAACt                                                                                                   >  1:810265/1‑177 (MQ=255)                                                                                                                                                                                                              caTTAAAACCGGATACCAGAATCATATCCTTTTTGCGATCCACAATGCGTAAACTGTAATCCTCGTCCATAACCACAATGTCGCCGGTCGCCATCCAGCCGTCTTTAATCACTTCCGCAGTGGCTTCCGGGCGCTGCCAATAACCTTGCATAACCTGCTCGCCTTTCACCCATAACTCGCCCGCTTCGCCGATAGCCGCTTCCGTGTCATCTTCTTtaat                                                        <  1:753867/220‑1 (MQ=255)                                                                                                                                                                                                                     aCCGGATACCAGAATCATATCCTTTTTGCGATCCACAATGCGTAAACTGTAATCCTCGTCCATAACCACAATGTCGCCGGTCGCCATCCAGCCGTCTTTAATCACTTCCGCAGTGGCTTCCGGGCGCTGCCAATAACCTTGCATAACCTGCTCGCCTTTCACCCATAACTCGCCCGCTTCGCCGATAGCCGCTTCCGTGTCATCTTCTTTAATAATTTTAATGTCCGTATTCGGCACCGGCACACCGATAc                  <  1:608541/251‑1 (MQ=255)                                                                                                                                                                                                                     aCCGGATACCAGAATCATATCCTTTTTGCGATCCACAATGCGTAAACTGTAATCCTCGTCCATAACCACAATGTCGCCGGTCGCCATCCAGCCGTCTTTAATCACTTCCGCAGTGGCTTCCGGGCGCTGCCAATAACCTTGCATAACCTGCTCGCCTTTCACCCATAACTCGCCCGCTTCGCCGATAGCCGCTTCCGTGTCATCTTCTTTAATAATTTTAATGTCCGTATTCGGCACCGGCACACCGATAc                  <  1:343078/251‑1 (MQ=255)                                                                                                                                                                                                                         gATACCAGAATCATATCCTTTTTGCGATCCACAATGCGTAAACTGTAATCCTCGTCCATAACCACAATGTCGCCGGTCGCCATCCAGCCGTCTTTAATCACTTCCGCAGTGGCTTCCGGGCGCTGCCAATAACCTTGCATAACCTGCTCGCCTTTCACCCATAACTCGCCCGCTTCGCCGATAGCCGCTTCCGTGTCATCTTCTTTAATAATTTTAATGTCCGTATTCGGCACCGGCACACCGATACTGc               <  1:43637/250‑1 (MQ=255)                                                                                                                                                                                                                             ccAGAATCATATCCTTTTTGCGATCCACAATGCGTAAACTGTAATcc                                                                                                                                                                                                                      <  1:27449/47‑1 (MQ=255)                                                                                                                                                                                                                             ccAGAATCATATCCTTTTTGCGATCCACAATGCGTAAACTGTAATCCTCGTCCATAACCACAATGTCGCCGGTCGCCATCCAGCCGTCTTTAATCACTTCCGCAGTGGCTTCCGGGCGCTGCCAATAACCTTGCAt                                                                                                                             <  1:685978/136‑1 (MQ=255)                                                                                                                                                                                                                                gAATCATATCCTTTTTGCGATCCACAATGCGTAAACTGTAATCCTCGTCCATAACCACAATGTCACCGGTCGCCATCCAGCCGTCTTTAATCACTTCCGCAGTGGCTTCCGGGCGCTGCCAATAACCTTCCATAAcc                                                                                                                         >  1:348676/1‑137 (MQ=255)                                                                                                                                                                                                                                 aaTCATATCCTTTTTGCGATCCACAATGCGTAAGCTGTAATCCTCGTCCATAACCACAGTGTGGCCGGTCGCCATCCAGCCGTCTTTAATCACTTCCGCAGTGGCTTCCGGGCGCTGCCAATAACCTTGCATAACCTGCTCGCCTTTCACCCATAACTCGCCCGCTTCGCCGATAGCCGCTTCCGTGTCATCTTCTTTAATAATTTTAATGTCCGTATTCGGCACCGGCACACCGATACTGCCGTTATg        <  1:150500/249‑1 (MQ=255)                                                                                                                                                                                                                                 aaTCATATCCTTTTTGCGATCCACAATGCGTAAACTGTAATCCTCGTCCATAACCACAATGTCGCCGGTCGCCATCCAGCCGTCTTTAATCACTTCCGCAGTGGCTTCCGGGCGCTGCCAATAACCTTGCATAACCTGCTCGCCTTTCACCCATAACTCGCCCGCTTCGCCGATAGCCGCTTCCGTGTCATCTTCTTTAATAATTTTAATGTCCGTATTCGGCACCGGCACACCGATACTGCCGTTATg        <  1:763256/249‑1 (MQ=255)                                                                                                                                                                                                                                    catatcctTTTTGCGATCCACAATGCGTAAACTGTAATCCTCGTCCATAACCACAATGTCGCCGGTCGCCATCCAGCCGTCTTTAATCACTTCCGCAGTGGCTTCCGGGCGCTGCCAATAACCTTGCATAACCTGCTCGCCTTTCACCCATAACTCGCCCGCTTCGCCGATAGCCGCTTCCGTGTCATCTTCTTTAATAATTTTAATGTCCGTATTcggc                                  <  1:249126/220‑1 (MQ=255)                                                                                                                                                                                                                                     atatcctTTTTGCGATCCACAATGCGTAAACTGTAATCCTCGTCCATAACCACAATGTCGCCGGTCGCCATCCAGCCGTCTTTAATCACTTCCGCAGTGGCTTCCGGGCGCTGCCAATAACCTTGCATAACCTGCTCGCCTTTCACCCATAACTCGCCCGCTTCGCCGATAGCCGCTTCCGTGTCATCTTCTTTAATAATTTTAATGTCCGTATTCGGCACCGGCACACCGATACTGCCGTTATGTTTcac  <  1:185875/251‑1 (MQ=255)                                                                                                                                                                                                                                     atatcctTTTTGCGATCCACAATGCGTAAACTGTAATCCTCGTCCATAACCACAATGTCGCCGGTCGCCATCCAGCCGTCTTTAATCACTTCCGCAGTGGCTTCCGGGCGCTGCCAATAACCTTGCATAACCTGCTCGCCTTTCACCCATAACTCGCCCGCTTCGCCGATAGCCGCTTCCGTGTCATCTTCTTTAATAATTTTAATGTCCGTATTCGGCACCGGCACACCGATACTGCCGTTATGTTTcac  <  1:579431/251‑1 (MQ=255)                                                                                                                                                                                                                                     atatcctTTTTGCGATCCACAATGCGTAAACTGTAATCCTCGTCCATAACCACAATGTCGCCGGTCGCCATCCAGCCGTCTTTAATCACTTCCGCAGTGGCTTCCGGGCGCTGCCAATAACCTTGCATAACCTGCTCGCCTTTCACCCATAACTCGCCCGCTTCGCCGATAGCCGCTTCCGTGTCATCTTCTTTAATAATTTTAATGTCCGTATTCGGCACCGGCACACCGATACTGCCGTTATGTTTcac  <  1:73728/251‑1 (MQ=255)                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                                      CGGAACTCAATATCTTTCGGTATTTTGTAACCCGTTAGGTGTTCGCGGCAATGCGTACGCAGTTCATCGCGGGTCAGGCTTTCGTCTTTTTTCACCACAAAAAGCTTAATGGTTTCGCCGGAAACCTCATGAGGCACGCCGATGGCAACCACTTCGGAAACCTTGTTATTCAACATCACCACATCTTCGATTTCATTTGGATACACATTAAAACCGGATACCAGAATCATATCATTTTTGCGATCCACAATGCGTAAACTGTAATCCTCGTCCATAACCACAATGTCGCCGGTCGCCATCCAGCCGTCTTTAATCACTTCCGCAGTGGCTTCCGGGCGCTGCCAATAACCTTGCATAACCTGCTCGCCTTTCACCCATAACTCGCCCGCTTCGCCGATAGCCGCTTCCGTGTCATCTTCTTTAATAATTTTAATGTCCGTATTCGGCACCGGCACACCGATACTGCCGTTATGTTTCAC  >  1/1808186‑1808664

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑