Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,769,379	C→T	intergenic (+49421/‑25463)	unknown → / → unknown	tRNA‑Asn‑GTT/tRNA‑Leu‑CAA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,769,379	0	C	T	100.0%	213.1 / NA	76	intergenic (+49421/‑25463)	unknown/unknown	tRNA‑Asn‑GTT/tRNA‑Leu‑CAA
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (43/33);  total (43/33)

GTGGAAACACAGTTACAAGTAAACAAACCTAAACTTTACAGCTGGCTTGCATTCTGTTTACAAAAAATTCAAGAATTACGCGTCATTAAGACCGCACTTGAGCAAGGCAGAGCCGCCGTACAAGCGGAACTGGATGCCAGCCAAGTTGCCGCCGATGCCCGTGCGACCTCAAAAGATATTCATCGCCCTGAAGTGGCGGAACGTTTGGCGAACTTGCCAAAAGGCGCGGATCAACGTAAATCCCCGTTTGCGGAACGTATCAAATTACAAAATGCCTGGTTAAATCTACCGTTATTGCCAACCACGAATATCGGTTCTTTCCCGCAAACTACTGAAATTCGACACGCGCGCGCCGCCTTCAAAAAAGGCACATTGTCCCTTGCGGATTATGAAGCGGCAATGAAAAAAGAAATCGAATTGGTGGTGCGTGAGCAAGAAAAATTGGATTTAGACGTGCTGGTTCACGGCGAAGCGGAACGTAACGACATGG  >  1/1769136‑1769625                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                       gTGGAAACACAGTTACAAGTAAACAAACCTAAACTTTACAGCTGGCTTGCATTCTGTTTACAAAAAATTCAAGAATTACGCGTCATTAAGACCGCACTTGAGCAAGGCAGAGCCGCCGTACAAGCGGAACTGGATGCCAGCCAAGTTGCCGCCGATGCCCGTGCGACCTCAAAAGATATTCATCGCCCTGAAGTGGCGGAACGTTTGGCGAACTTGCCAAAAGGCGCGGATCAACGTAAATCCTCGTTTg                                                                                                                                                                                                                                                  >  1:624371/1‑250 (MQ=255) gTGGAAACACAGTTACAAGTAAACAAACCTAAACTTTACAGCTGGCTTGCATTCTGTTTACAAAAAATTCAAGAATTACGCGTCATTAAGACCGCACTTGAGCAAGGCAGAGCCGCCGTACAAGCGGAACTGGATGCCAGCCAAGTTGCCGCCGATGCCCGTGCGACCTCAAAAGATATTCATCGCCCTGAAGTGGCGGAACGTTTGGCGAACTTGCCAAAAGGCGCGGATCAACGTAAATCCTCGTTTg                                                                                                                                                                                                                                                  >  1:231612/1‑250 (MQ=255)      aaCACAGTTACAAGTAAACAAACCTAAACTTTACAGCTGGCTTGCATTCTGTTTACAAAAAATTCAAGAATTACGCGTCATTAAGACCGCACTTGAGCAAGGCAGAGCCGCCGTACAAGCGGAACTGGATGCCAGCCAAGTTGCCGCCGATGCCCGTGCGACCTCAAAAGATATTCATCGCCCTGAAGTGGCGGAACGTTTGGCGAACTTGCCAAAAGGCGCGGATCAACGTAAATCCTCGTTTGCGGaa                                                                                                                                                                                                                                             >  1:380226/1‑250 (MQ=255)            gTTACAAGTAAACAAACCTAAACTTTACAGCTGGCTTGCATTCTGTTTACAAAAAATTCAAGAATTACGCGTCATTAAGACCGCACTTGAGCAAGGCAGAGCCGCCGTACAAGCGGAACTGGATGCCAGCCAAGTTGCCGCCGATGCCCGTGCGACCTCAAAAGATATTCATCGCCCTGAAGTGGCGGAACGTTTGGCGAACTTGCCAAAAGGCGCGGATCAACGTAAATCCTCGTTTGCGGAACGTATCa                                                                                                                                                                                                                                      >  1:177559/1‑251 (MQ=255)               aCAAGTAAACAAACCTAAACTTTACAGCTGGCTTGCATTCTGTTTACAAAAAATTCAAGAATTACGCGTCATTAAGACCGCACTTGAGCAAGGCAGAGCCGCCGTACAAGCGGAACTGGATGCCAGCCAAGTTGCCGCCGATGCCCGTGCGACCTCAAAAGATATTCATCGCCCTGAAGTGGCGGAACGTTTGGCGAACTTGCCAAAAGGCGCGGATCAACGTAAATCCTCGTTTGCGGAACGTATCAAAt                                                                                                                                                                                                                                   <  1:356011/251‑1 (MQ=255)                       acaaacCTAAACTTTACAGCTGGCTTGCATTCTGTTTACAAAAAATTCAAGAATTACGCGTCATTAAGACCGCACTTGAGCAAGGCAGAGCCGCCGTACAAGCGGAACTGGATGCCAGCCAAGTTGCCGCCGATGCCCGTGCGACCTCAAAAGATATTCATCGCCCTGAAGTGGCGGAACGTTTGGCGAACTTGCCAAAAGGCGCGGATCAACGTAAATCCTCGTTTGCGGAACGTATCAAATTACAAAAt                                                                                                                                                                                                                           >  1:711447/1‑251 (MQ=255)                         aaacCTAAACTTTACAGCTGGCTTGCATTCTGTTTACAAAAAATTCAAGAATTACGCGTCATTAAGACCGCACTTGAGCAAGGCAGAGCCGCCGTACAAGCGGAACTGGATGCCAGCCAAGTTGCCGCCGATGCCCGTGCGACCTCAAAAGATATTCATCGCCCTGAAGTGGCGGAACGTTTGGCGAACTTGCCAAAAGGCGCGGATCAACGTAAATCCTCGTTTGCGGAACGTATCAAATTACAAAATGc                                                                                                                                                                                                                         <  1:662959/251‑1 (MQ=255)                          aacCTAAACTTTACAGCTGGCTTGCATTCTGTTTACAAAAAATTCAAGAATTACGCGTCATTGAGACCGCACTTGAGCAAGGCAGAGCCGCCGTACAAGCGGAACTGGATGCCAGCCAAGTTGCCGCCGATGCCCGTGCGACCTCAAAAGATATTCATCGCCCTGAAGTGGCGGAACGTTTGGCGAACTTGCCAAAAGGCGCGGATCAACGTAAATCCTCGTTTGCGGAACGCATCAAATTACAAAATGc                                                                                                                                                                                                                         >  1:517938/1‑250 (MQ=255)                          aacCTAAACTTTACAGCTGGCTTGCATTCTGTTTACAAAAAATTCAAGAATTACGCGTCATTAAGACCGCACTTGAGCAAGGCAGAGCCGCCGTACAAGCGGAACTGGATGCCAGCCAAGTTGCCGCCGATGCCCGTGCGACCTCAAAAGATATTCATCGCCCTGAAGTGGCGGAACGTTTGGCGAACTTGCCAAAAGGCGCGGATCAACGTAAATCCTCGTTTGCGGAACGTATCAAATTACAAAATGcc                                                                                                                                                                                                                        <  1:230127/251‑1 (MQ=255)                                  cTTTACAGCTGGCTTGCATTCTGTTTACAAAAAATTCAAGAATTACGCGTCATTAAGACCGCACTTGAGCAAGGCAGAGCCGCCGTACAAGCGGAACTGGATGCCAGCCAAGTTGCCGCCGATGCCCGTGCGACCTCAAAAGATATTCATCGCCCTGAAGTGGCGGAACGTTTGGCGAACTTGCCAAAAGGCGCGGATCAACGTAAATCCTCGTTTGCGGAACGTATCAAATTACAAAATGCCTGGTTaaa                                                                                                                                                                                                                >  1:461423/1‑251 (MQ=255)                                  cTTTACAGCTGGCTTGCATTCTGTTTACAAAAAATTCAAGAATTACGCGTCATTAAGACCGCACTTGAGCAAGGCAGAGCCGCCGTACAAGCAGAACTGGATGCCAGCCAAGTTGCCGCCGATGCCCGTGCGACCTCAAAAGATATTCATCGCCCTGAAGTGGCGGAACGTTTGGCGAACTTGCCAAAAGGCGCGGATCAACGTAAATCCTCGCTTGCGGAACGTATCAAATTACAAAATGCCTGGTTaaa                                                                                                                                                                                                                >  1:481818/1‑251 (MQ=255)                                   tttACAGCTGGCTTGCATTCTGTTTACAAAAAATTCAAGAATTACGCGTCATTAAGACCGCACTTGAGCAAGGCAGAGCCGCCGTACAAGCGGAACTGGATGCCAGCCAAGTTGCCGCCGATGCCCGTGCGACCTCAAAAGATATTCATCGCCCTGAAGTGGCGGAACGTTTGGCGAACTTGCCAAAAGGCGCGGATCAACGTAAATCCTCGTTTGCGGAACGTATCAAATTACAAAATGCCTGGTTAAAt                                                                                                                                                                                                               <  1:187002/251‑1 (MQ=255)                                          cTGGCTTGCATTCTGTTTACAAAAAATTCAAGAATTACGCGTCATTAAGACCGCACTTGAGCAAGGCAGAGCCGCCGTACAAGCGGAACTGGATGCCAGCCAAGTTGCCGCCGATGCCCGTGCGACCTCAAAAGATATTCATCGCCCTGAAGTGGCGGAACGTTTGGCGAACTTGCCAAAAGGCGCGGATCAACGTAAATCCTCGTTTGCGGAACGTATCAAATTACAAAATGCCTGGTTAAATCTACCg                                                                                                                                                                                                         <  1:880608/250‑1 (MQ=255)                                            ggCTTGCATTCTGTTTACAAAAAATTCAAGAATTACGCGTCATTAAGACCGCACTTGAGCAAGGCAGAGCCGCCGTACAAGCGGAACTGGATGCCAGCCAAGTTGCCGCCGATGCCCGTGCGACCTCAAAAGATATTCATCGCCCTGAAGTGGCGGAACGTTTGGCGAACTTGCCAAAAGGCGCGGATCAACGTAAATCCTCGTTTGCGGAACGTATCAAATTACAAAATGCCTGGTTAAATCTACCGTTa                                                                                                                                                                                                      >  1:858097/1‑251 (MQ=255)                                              cTTGCATTCTGTTTACAAAAAATTCAAGAATTACGCGTCATTAAGACCGCACTTGAGCAAGGCAGAGCCGCCGTACAAGCGGAACTGGATGCCAGCCAAGTTGCCGCCGATGCCCGTGCGACCTCAAAAGATATTCATCGCCCTGAAGTGGCGGAACGTTTGGCGAACTTGCCAAAAGGCGCGGATCAACGTAAATCCTCGTTTGCGGAACGTATCAAATTACAAAATGCCTGGTTAAATCTACCGTTAtt                                                                                                                                                                                                    >  1:118751/1‑251 (MQ=255)                                                       tGTTTACAAAAAATTCAAGAATTACGCGTCATTAAGACCGCACTTGAGCAAGGCAGAGCCGCGGTACAAGCGGAACTGGATGCCAGCCAAGTTGCCGCCGATGCCCGTGCGACCTCAAAAGATATTCATCGCCCTGAAGTGGCGGAACGTTTGGCGAACTTGCCAAAAGGCGCGGATCAACGTAAATCCTCGTTTGCGGAACGTATCAAATTACAAAATGCCTGGTTAAATCTACCGTTATTGCCAACCAc                                                                                                                                                                                           <  1:202162/251‑1 (MQ=255)                                                             cAAAAAATTCAAGAATTACGCGTCATTAAGACCGCACTTGAGCAAGGCAGAGCCGCCGTACAAGCGGAACTGGATGCCAGCCAAGTTGCCGCCGATGCCCGTGCGACCTCAAAAGATATTCATCGCCCTGAAGTGGCGGAACGTTTGGCGAACTTGCCAAAAGGCGCGGATCAACGTAAATCCTCGTTTGCGGAACGTATCAAATTACaaaa                                                                                                                                                                                                                            >  1:871208/1‑212 (MQ=255)                                                                           aTTACGCGTCATTAAGCCCGCACTTGAGCAAGGCAGAGCCGCCGTACAAGCGGAACTGGATGCCAGCCAAGTTGCCGCCGATGCCCGTGCGACCTCAAAAGATATTCATCGCCCTGAAGTGGCGGAACGTTTGGCGAACTTGCCAAAAGGCGCGGATCAACGTAAATCCTCGTTTGCGGAACGTATCAAATTACAAAATGCCTGGTTAAATCTACCGTTATTGCCAACCACGAATATCGGTTCTTTCCCGc                                                                                                                                                                       <  1:131905/251‑1 (MQ=255)                                                                                   tCATTAAGACCGCACTTGAGCAAGGCAGAGCCGCCGTACAAGCGGAACTGGATGCCAGCCAAGTTGCCGCCGATGCCCGTGCGACCTCAAAAGATATTCATCGCCCTGAAGTGGCGGAACGTTTGGCGAACTTGCCAAAAGGCGCGGATCAACGTAAATCCTCGTTTGCGGAACGTATCAAATTACAAAATGCCTGGTTAAATCTACCg                                                                                                                                                                                                         <  1:282394/209‑1 (MQ=255)                                                                                   tCATTAAGACCGCACTTGAGCAAGGCAGAGCCGCCGTACAAGCGGAACTGGATGCCAGCCAAGTTGCCGCCGATGCCCGTGCGACCTCAAAAGATATTCATCGCCCTGAAGTGGCGGAACGTTTGGCGAACTTGCCAAAAGGCGCGGATCAACGTAAATCCTCGTTTGCGGAACGTATCAAATTACAAAATGCCTGGTTAAATCTACCGTTa                                                                                                                                                                                                      >  1:740109/1‑212 (MQ=255)                                                                                   tCATTAAGACCGCACTTGAGCAAGGCAGAGCCGCCGTACAAGCGGAACTGGATGCCAGCCAAGTTGCCGCCGATGCCCGTGCGACCTCAAAAGATATTCATCGCCCTGAAGTGGCGGAACGTTTGGCGAACTTGCCAAAAGGCGCGGATCAACGTAAATCCTCGTTTGCGGAACGTATCAAATTACAAAATGCCTGGTTAAATCTACCGTTATTGCCAACCAc                                                                                                                                                                                           >  1:640965/1‑223 (MQ=255)                                                                                   tCATTAAGACCGCACTTGAGCAAGGCAGAGCCGCCGTACAAGCGGAACTGGATGCCAGCCAAGTTGCCGCCGATGCCCGTGCGACCTCAAAAGATATTCATCGCCCTGAAGTGGCGGAACGTTTGGCGAACTTGCCAAAAGGCGCGGATCAACGTAAATCCTCGTTTGCGGAACGTATCAAATTACAAAATGCCTGGTTAAATCTACCGTTATTGCCAACCACGAATATCGGTTCTTTCCCGCAAacta                                                                                                                                                                 >  1:741013/1‑249 (MQ=255)                                                                                   tCATTAAGACCGCACTTGAGCAAGGCAGAGCCGCCGTACAAGCGGAACTGGATGCCAGCCAAGTTGCCGCCGATGCCCGTGCGACCTCAAAAGATATTCATCGCCCTGAAGTGGCGGAACGTTTGGCGAACTTGCCAAAAGGCGCGGATCAACGTAAATCCTCGTTTGCGGAACGTATCAAATTACAAAATGCCTGGTTAAATCTACCGTTATTGCCAACCACGAATATCGGTTCTTTCCCGCAAacta                                                                                                                                                                 >  1:563657/1‑249 (MQ=255)                                                                                   tCATTAAGACCGCACTTGAGCAAGGCAGAGCCGCCGTACAAGCGGAACTGGATGCCAGCCAAGTTGCCGCCGATGCCCGTGCGACCTCAAAAGATATTCATCGCCCTGAAGTGGCGGAACGTTTGGCGAACTTGCCAAAAGGCGCGGATCAACGTAAATCCTCGTTTGCGGAACGTATCAAATTACAAAATGCCTGGTTAAATCTACCGTTATTGCCAACCACGAATATCGGTTCTTTCCCGCAAacta                                                                                                                                                                 >  1:164288/1‑249 (MQ=255)                                                                                    cATTAAGACCGCACTTGAGCAAGGCAGAGCCGCCGTACAAGCGGAACTGGATGCCAGCCAAGTTGCCGCCGATGCCCGTGCGACCTCAAAAGATATTCATCGCCCTGAAGTGGCGGAACGTTTGGCGAACTTGCCAAAAGGCGCGGATCAACGTAAATCCTCGTTTGCGGAACGTATCAAATTACAAAATGCCTGGTTAAATCTACCGTTa                                                                                                                                                                                                      >  1:709866/1‑211 (MQ=255)                                                                                       tAAGACCGCACTTGAGCAAGGCAGAGCCGCCGTACAAGCGGAACTGGATGCCAGCCAAGTTGCCGCCGATGCCCGTGCGACTTCAAAAGATATTCATCGCCCTGAAGTGGCGGAACGTTTGGCGAACTTGCCAAAAGGCGCGGATCAACGTAAATCCTCGTTTGCGGAACGTATCAAATTACAAAATGCCTGGTTAAATCTACCGTTATTGCCAACCACGAATATCGGTTCTTTCCCGCAAACTACTGaaa                                                                                                                                                           <  1:453822/251‑1 (MQ=255)                                                                                       tAAGACCGCACTTGAGCAAGGCAGAGCCGCCGTACAAGCGGAACTGGATGCCAGCCAAGTTGCCGCCGATGCCCGTGCGACCTCAAAAGATATTCATCGCCCTGAAGTGGCGGAACGTTTGGCGAACTTGCCAAAAGGCGCGGATCAACGTAAATCCTCGTTTGCGGAACGTATCAAATTACAAAATGCCTGGTTAAATCTACCGTTATTGCCAACCACGAATATCGGTTCTTTCCCGCAAACTACTGaaa                                                                                                                                                           <  1:887176/251‑1 (MQ=255)                                                                                         aGACCGCACTTGAGCAAGGCAGAGCCGCCGTACAAGCGGAACTGGATGCCAGCCAAGTTGCCGCCGATGCCCGTGCGACCTCAAAAGATATTCATCGCCCTGAAGTGGCGGAACGTTTGGCGAACTTGCCAAAAGGCGCGGATCAACGTAAATCCTCGTTTGCGGAACGTATCAAATTACAAAATGCCTGGTTAAATCTACCGTTATTGCCAACCACGAATATCGGTTCTTTCCCGCAAACTACTGAAAtt                                                                                                                                                         >  1:431704/1‑251 (MQ=255)                                                                                           aCCGCACTTGAGCAAGGCAGAGCCGCCGTACAAGCGGAACTGGATGCCAGCCAAGTTGCCGCCGATGCCCGTGCGACCTCAAAAGATATTCATCGCCCTGAAGTGGCGGAACGTTTGGCGAACTTGCCAAAAGGCGCGGATCAACGTAAATCCTCGTTTGCGGAACGTATCAAATTACAAAATGCCTGGTTAAATCTACCGTTATTGCCAACCACGAATATCGGTTCTTTCCCGCAAACTACTGAAAtt                                                                                                                                                         <  1:167559/249‑1 (MQ=255)                                                                                              gCACTTGAGCAAGGCAGAGCCGCCGTACAAGCGGAACTGGATGCCAGCCAAGTTGCCGCCGATGCCCGTGCGACCTCAAAAGATATTCATCGCCCTGAAGTGGCGGAACGTTTGGCGAACTTGCCAAAAGGCGCGGATCAACGTAAATCCTCGTTTGCGGAACGTATCAAAtt                                                                                                                                                                                                                                  >  1:228895/1‑173 (MQ=255)                                                                                              gCACTTGAGCAAGGCAGAGCCGCCGTACAAGCGGAACTGGATGCCAGCCAAGTTGCCGCCGATGCCCGTGCGACCTCAAAAGATATTCATCGCCCTGAAGTGGCGGAACGTTTGGCGAACTTGCCAAAAGGCGCGGATCAACGTAAATCCTCGTTTGCGGAACGTATCAAATTACAAAATGCCTGGTTAAATCTACCGTTATTGCCAACCACGAATATCGGTTCTTTCCCGCAAACTACTGAAATTCGac                                                                                                                                                     >  1:47313/1‑250 (MQ=255)                                                                                               cACTTGAGCAAGGCAGAGCCGCCGTACAAGCGGAACTGGATGCCAGCCAAGTTGCCGCCGATGCCCGTGCGACCTCAAAAGATATTCATCGCCCTGAAGTGGCGGAACGTTTGGCGAACTTGCCAAAAGGCGCGGATCAACGTAAATCCTCGTTTGCGGAACGTATCAAATTACAAAATGCCTGGTTAAATCTACCGTTATTGCCAACCACGAATATCGGTTCTTTCCCGCAAACTACTGAAATTCGaca                                                                                                                                                    >  1:559170/1‑250 (MQ=255)                                                                                               cACTTGAGCAAGGCAGAGCCGCCGTACAAGCGGAACTGGATGCCAGCCAAGTTGCCGCCGATGCCCGTGCGACCTCAAAAGATATTCATCGCCCTGAAGTGGCGGAACGTTTGGCGAACTTGCCAAAAGGCGCGGATCAACGTAAATCCTCGTTTGCGGAACGTATCAAATTACAAAATGCCTGGTTAAATCTACCGTTATTGCCAACCACGAATATCGGTTCTTTCCCGCAAACTACTGAAATTCGaca                                                                                                                                                    <  1:539238/250‑1 (MQ=255)                                                                                                aCTTGAGCAAGGCAGAGCCGCCGTACAAGCGGAACTGGATGCCAGCCAAGTTGCCGCCGATGCCCGTGCGACCTCAAAAGATATTCATCGCCCTGAAGTGGCGGAACGTTTGGCGAACTTGCCAAAAGGCGCGGATCAACGTAAATCCTCGTTTGCGGAACGTATCAAATTACAAAATGCCTGGTTAAATCTACCGTTATTGCCAACCACGAATATCGGTTCTTTCCCGCAAACTACTGAAATTCGACAcg                                                                                                                                                  <  1:579113/251‑1 (MQ=255)                                                                                                                   gccgTACAAGCGGAACTGGATGCCAGCCAAGTTGCCGCCGATGCCCGTGCGACCTCAAAAGATATTCATCGCCCTGAAGTGGCGGAACCTTTGGCGAACTTGCCAAAACGCGCGGATCAACGTAAATCCTCGTTTGCGGAACGTATCAAATTACAAAATGCCTGGTTAAATCTACCGTTATTGCCAACCACGAATATCGGTTCTTTCCCGCAAACTACTGAAATTCGACACGCGCGCGCCGCCTTCaaaaa                                                                                                                               >  1:851475/1‑251 (MQ=255)                                                                                                                      gTACAAGCGGAACTGGATGCCAGCCAAGTTGCCGCCGATGCCCGTGCGACCTCAAAAGATATTCATCGCCCTGAAGTGGCGGAACGTTTGGCGAACTTGCCAAAAGGCGCGGATCAACGTAAATCCTCGTTTGCGGAACGTATCAAATTACAAAATGCCTGGTTAAATCTACCGTTAtt                                                                                                                                                                                                    >  1:685879/1‑179 (MQ=255)                                                                                                                      gTACAAGCGGAACTGGATGCCAGCCAAGTTGCCGCCGATGCCCGTGCGACCTCAAAAGATATTCATCGCCCTGAAGTGGCGGAACGTTTGGCGAACTTGCCAAAAGGCGCGGATCAACGTAAATCCTCGTTTGCGGAACGTATCAAATTACAAAATGCCTGGTTAAATCTACCGTTATTGCCAACCACGAATATCGGTTCTTTCCCGCAAACTACTGAAATTCGACACGCGCGCGCCGCCTTCAAAAAAgg                                                                                                                            >  1:569716/1‑251 (MQ=255)                                                                                                                       tACAAGCGGAACTGGATGCCAGCCAAGTTGCCGCCGATGCCCGTGCGACCTCAAAAGATATTCATCGCCCTGAAGTGGCGGAACGTTTGGCGAACTTGCCAAAAGGCGCGGATCAACGTAAATCCTCGTTTGCGGAACGTATCAAATTACAAAATGCCTGGTTAAATCTACCGTTATTGCCAACCACGAATATCGGTTCTTTCCCGCAAACTACTGAAATTCGACACGCGCGCGCCGCCTTCAAAAAAgg                                                                                                                            <  1:153591/250‑1 (MQ=255)                                                                                                                               gAACTGGATGCCAGCCAAGTTGCCGCCGATGCCCGTGCGACCTCAAAAGATATTCATCGCCCTGAAGTGGCGGAACGTTTGGCGAACTTGCCAAAAGGCGCGGATCAACGTAAATCCTCGTTTGCGGAACGTATCAAATTACAAAATGCCTGGTTAAATCTACCGTTATTGCCAACCACGAATATCGGTTCTTTCCCGCAAACTACTGAAATTCGACACGCGCGCGCCGCCTTCAAAAAAGGCACATTGt                                                                                                                    >  1:426366/1‑250 (MQ=255)                                                                                                                                aaCTGGATGCCAGCCAAGTTGCCGCCGATGCCCGTGCGACCTCAAAAGATATTCATCGCCCTGAAGTGGCGGAACGTTTGGCGAACTTGCCAAAAGGCGCGGATCAACGTAAATCCTCGTTTGCGGAACGTATCAAATTACAAAATGCCTGGTTAAATCTACCGTTATTGCCAACCACGAATATCGGTTCTTTCCCGCAGACTACTGAAATTCGACACGCGCGCGCCGCCTTCAAAAAAGGCACATTGTcc                                                                                                                  <  1:405156/251‑1 (MQ=255)                                                                                                                                aaCTGGATGCCAGCCAAGTTGCCGCCGATGCCCGTGCGACCTCAAAAGATATTCATCGCCCTGAAGTGGCGGAACGTTTGGCGAACTTGCCAAAAGGCGCGGATCAACGTAAATCCTCGTTTGCGGAACGTATCAAATTACAAAATGCCTGGTTAAATCTACCGTTATTGCCAACCACGAATATCGGTTCTTTCCCGCAAACTACTGAAATTCGACACGCGCGCGCCGCCTTCAAAAAAGGCACATTGTcc                                                                                                                  <  1:322816/251‑1 (MQ=255)                                                                                                                                         ccagccaAGTTGCCGCCGATGCCCGTGCGACCTCAAAAGATATTCATCGCCCTGAAGTGGCGGAACGTTTGGCGAACTTGCCAAAAGGCGCGGATCAACGTAAATCCTCGTTTGCGGAACGTATCAAATTACaaaa                                                                                                                                                                                                                            <  1:571966/136‑1 (MQ=255)                                                                                                                                         ccagccaAGTTGCCGCCGATGCCCGTGCGACCTCAAAAGATATTCATCGCCCTGAAGTGGCGGAACGTTTGGCGAACTTGCCAAAAGGCGCGGATCAACGTAAATCCTCGTTTGCGGAACGTATCAAATTACAAAATGCCTGGTTAAATCTACCGTTATTGCCAACCACGAATATCGGTTCTTTCCCGCAAACTACTGAAATTCGACACGCGCGcgccg                                                                                                                                         <  1:44537/219‑1 (MQ=255)                                                                                                                                         ccagccaAGTTGCCGCCGATGCCCGTGCGACCTCAAAAGATATTCATCGCCCTGAAGTGGCGGAACGTTTGGCGAACTTGCCAAAAGGCGCGGATCAACGTAAATCCTCGTTTGCGGAACGTATCAAATTACAAAATGCCTGGTTAAATCTACCGTTATTGCCAACCACGAATATCGGTTCTTTCCCGCAAACTACTGAAATTCGACACGCGCGCGCCGCCTTCAAAAAAGGCACATTGTCCCTTGCGGAt                                                                                                         <  1:791939/251‑1 (MQ=255)                                                                                                                                         ccagccaAGTTGCCGCCGATGCCCGTGCGACCTCAAAAGATATTCATCGCCCTGAAGTGGCGGAACGTTTGGCGAACTTGCCAAAAGGCGCGGATCAACGTAAATCCTCGTTTGCGGAACGTATCAAATTACAAAATGCCTGGTTAAATCTACCGTTATTGCCAACCACGAATATCGGTTCTTTCCCGCAAACTACTGAAATTCGACACGCGCGCGCCGCCTTCAAAAAAGGCACATTGTCCCTTGCGGAt                                                                                                         <  1:649663/251‑1 (MQ=255)                                                                                                                                              caAGTTGCCGCCGATGCCCGTGCGACCTCAAAAGATATTCATCGCCCTGAAGTGGCGGAACGTTTGGCGAACTTGCCAAAAGGCGCGGATCAACGTAAATCCTCGTTTGCGGAACGTATCAAATTACAAAATGCCTGGTTAAATCTACCGTTATTGCCAACCACGAATATCGGTTCTTTCCCGCAAACTACTGAAATTCGACACGCGCGCGCCGCCTTCAAAAAAGGCACATTGTCCCTTGCGGATTATGa                                                                                                    <  1:603333/251‑1 (MQ=255)                                                                                                                                                       gccgATGCCCGTGCGACCTCAAAAGATATTCATCGCCCTGAAGTGGCGGAACGTTTGGCGAACTTGCCAAAAGGCGCGGATCAACGTAAATCCTCGTTTGCGGAACGTATCAAATTACAAAATGCCTGGTTAAATCTACCGTTATTGcc                                                                                                                                                                                                 <  1:410727/149‑1 (MQ=255)                                                                                                                                                       gccgATGCCCGTGCGACCTCAAAAGATATTCATCGCCCTGAAGTGGCGGAACGTTTGGCGAACTTGCCAAAAGGCGCGGATCAACGTAAATCCTCGTTTGCGGAACGTATCAAATTACAAAATGCCTGGTTAAATCTACCGTTATTGCCAACCACGAATATCGGTTCTTTCCCGCAAACTACTGAAATTCGACACGCGCGCGCCGCCTTCAAAAAAGGCACATTGTCCCTTGCGGATTATGAAGCGGCAAt                                                                                           >  1:136972/1‑251 (MQ=255)                                                                                                                                                           aTGCCCGTGCGACCTCAAAAGATATTCATCGCCCTGAAGTGGCGGAACGTTTGGCGAACTTGCCAAAAGGCGCGGATCAACGTAAATCCTCGTTTGCGGAACGTATCAAATTACAAAATGCCTGGTTAAATCTACCGTTATTGCCAACCACGAATATCGGTTCTTTCCCGCAAACTACTGAAATTCGACACGCGCGCGCCGCCTTCAAAAAAGGCACATTGTCCCTTGCGGATTATGAAGCGGCAATGaaa                                                                                       >  1:277819/1‑251 (MQ=255)                                                                                                                                                              cccGTGCGACCTCAAAAGATATTCATCGCCCTGAAGTGGCGGAACGTTTGGCGAACTTGCCAAAAGGCGCGGATCAACGTAAATCCTCGTTTGCGGAACGTATCAAATTACAAAATGCCTGGTTAAATCTACCGTTATTGCCAACCACGAATATCGGTTCTTTCCCGCACACTACTGAAATTCGACACGCGCGCGCCGCCTTCAAAAAAGGCACATTGTCCCTTGCGGATTATGAAGCGGCAATGaaaaaa                                                                                    >  1:422993/1‑251 (MQ=255)                                                                                                                                                                      aCCTCAAAAGATATTCATCGCCCTGAAGTGGCGGAACGTTTGGCGAACTTGCCAAAAGGCGCGGATCAACGTAAATCCTCGTTTGCGGAACGTATCAAATTACAAAATGCCTGGTTAAATCTACCGTTATTGCCAACCACGAATATCGGTTCTTTCCCGCAAACTACTGAAATTCGACACGCGCGCGCCGCCTTCAAAAAAGGCACATTg                                                                                                                     <  1:334821/210‑1 (MQ=255)                                                                                                                                                                        cTCAAAAGATATTCATCGCCCTGAAGTGGCGGAACGTTTGGCGAACTTGCCAAAAGGCGCGGATCAACGTAAATCCTCGTTTGCGGAACGTATCAAATTACAAAATGCCTGGTTAAATCTACCGTTATTGCCAACCACGAATATCGGTTCTTTCCCGCAAACTACTGAACTTCGACACGCGCGCgccgcc                                                                                                                                       >  1:455853/1‑190 (MQ=255)                                                                                                                                                                        cTCAAAAGATATTCATCGCCCTGAAGTGGCGGAACGTTTGGCGAACTTGCCAAAAGGCGCGGATCAACGTAAATCCTCGTTTGCGGAACGTATCAAATTACAAAATGCCTGGTTAAATCTACCGTTATTGCCAACCACGAATATCGGTTCTTTCCCGCAAACTACTGAAATTCGACACGCGCGCGCCGCCTTCAAAAAAGGCACATTGTCCCTTGCGGATTATGAAGCGGCAATGAAAAAAGAAATCGAAt                                                                          >  1:709518/1‑251 (MQ=255)                                                                                                                                                                          cAAAAGATATTCATCGCCCTGAAGTGGCGGAACGTTTGGCGAACTTGCCAAAAGGCGCGGATCAACGTAAATCCTCGTTTGCGGAACGTATCAAATTACAAAATGCCTGGTTAAATCTACCGTTATTGCCAACCACGAATATCGGTTCTTTCCCGCAAACTACTGAAATTCGACACGCGCGCGCCGCCTTCAAAAAAGGCACATTGTCCCTTGCGGATTATGAAGCGGCAATGAAAAAAGAAATCGAATtg                                                                        >  1:821137/1‑251 (MQ=255)                                                                                                                                                                           aaaaGATATTCATCGCCCTGAAGTGGCGGAACGTTTGGCGAACTTGCCAAAAGGCGCGGATCAACGTAAATCCTCGTTTGCGGAACGTATCAAATTACAAAATGCCTGGTTAAATCTACCGTTATTGCCAACCACGAATATCGGTTCTTTCCCGCAAACTACTGAAATTCGACACGCGCGCGCCGCCTTCAAAAAAGGCACATTGTCCCTTGCGGATTATGAAGCGGCAATGCAAAAAGAAATCGAATtgg                                                                       >  1:823800/1‑251 (MQ=255)                                                                                                                                                                                atatTCATCGCCCTGAAGTGGCGGAACGTTTGGCGAACTTGCCAAAAGGCGCGGATCAACGTAAATCCTCGTTTGCGGAACGTATCAAATTACAAAATGCCTGGTTAAATCTACCGTTATTGCCAACCACGAATATCGGTTCTTTCCCGCAAACTACTGAAATTCGACACGCGCGCGCCGCCTTCAAAAAAGGCACATTGTCCCTTGCGGATTATGa                                                                                                    >  1:288667/1‑217 (MQ=255)                                                                                                                                                                                    tCATCGCCCTGAAGTGGCGGAACGTTTGGCGAACTTGCCAAAAGGCGCGGATCAACGTAAATCCTCGTTTGCGGAACGTATCAAATTACAAAATGCCTGGTTAAATCTACCGTTATTGcc                                                                                                                                                                                                 <  1:367377/120‑1 (MQ=255)                                                                                                                                                                                    tCATCGCCCTGAAGTGGCGGAACGTTTGGCGAACTTGCCAAAAGGCGCGGATCAACGTAAATCCTCGTTTGCGGAACGTATCAAATTACAAAATGCCTGGTTAAATCTACCGTTATTGCCAACCACGAATATCGGTTCTTTCCCGCAAACTACTGAAATTCGACACGCGCGCGCCGCCTTCAAAAAAGGCACATTGTCCCTTGCGGATTATGAAGCGGCAATGAAAAAAGAAATCGAATTGGTGGTGCGTg                                                              >  1:195502/1‑251 (MQ=255)                                                                                                                                                                                         gCCCTGAAGTGGCGGAACGTTTGGCGAACTTGCCAAAAGGCGCGGATCAACGTAAATCCTCGTTTGCGGAACGTATCAAATTACAAAATGCCTGGTTAAATCTACCGTTATTGCCAACCACGAATATCGGTTCTTTCCCGCAAACTACTGAAATTCGACACGCGCGCGCCGCCTTCAAAAAAGGCACATTGTCCCTTGCGGATTATGAAGCGGCAATGAAAAAAGAAATCGAATTGGTGGTGCGTGAGCaa                                                         >  1:413889/1‑251 (MQ=255)                                                                                                                                                                                          ctcTGAAGTGGCGGAACGTTTGGCGAACTTGCCAAAAGGCGCGGATCAACGTAAATCCTCGTTTGCGGAACGTATCAAATTACAAAATGCCTGGTTAAATCTACCGTTATTGCCAACCACGAATATCGGTTCTTTCCCGCAAACTACTGAAATTCGACACGCGCGCGCCGCCTTCAAAAAAGGCACATTGTCCCTTGCGGATTATGAAGCGGCAATGAAAAAAGAAATCGAATTGGTGGTGCGGGAGCAAg                                                        >  1:707907/3‑251 (MQ=255)                                                                                                                                                                                          cccTGAAGTGGCGGAACGTTTGGCGAACTTGCCAAAAGGCGCGGATCAACGTAAATCCTCGTTTGCGGAACGTATCAAATTACAAAATGCCTGGTTAAATCTACCGTTATTGCCAACCACGAATATCGGTTCTTTCCCGCAAACTACTGAAATTCGACACGCGCGCGCCGCCTTCAAAAAAGGCACATTGTCCCTTGCGGATTATGAAGCGGCAATGAAAAAAGAAATCGAATtggtgg                                                                    <  1:547952/239‑1 (MQ=255)                                                                                                                                                                                          cccTGAAGTGGCGGAACGTTTGGCGAACTTGCCAAAAGGCGCGGATCAACGTAAATCCTCGTTTGCGGAACGTATCAAATTACAAAATGCCTGGTTAAATCTACCGTTATTGCCAACCACGAATATCGGTTCTTTCCCGCAAACTACTGAAATTCGACACGCGCGCGCCGCCTTCAAAAAAGGCACATTGTCCCTTGCGGATTATGAAGCGGCAATGAAAAAAGAAATCGAATtggtgg                                                                    <  1:503765/239‑1 (MQ=255)                                                                                                                                                                                          cccTGAAGTGGCGGAACGTTTGGCGAACTTGCCAAAAGGCGCGGATCAACGTAAAGCCTCGTTTGCGGAACGTATCAAATTACAAAATGCCTGGTTAAATCTACCGTTATTGCCAACCACGAATATCGGTTCTTTCCCGCAAACTACTGAAATTCGACACGCGCGCGCCGCCTTCAAAAAAGGCACATTGTCCCTTGCGGATTATGAAGCGGCAATGAAAAAAGAAATCGAATTGGTGGTGCGTGAGCa                                                          <  1:700650/249‑1 (MQ=255)                                                                                                                                                                                                          cGTTTGGCGAACTTGCCAAAAGGCGCGGATCAACGTAAATCCTCGTTTGCGGAACGTATCAAATTACAAAATGCCTGGTTAAATCTACCGTTATTGCCAACCACGAATATCGGTTCTTTCCCGCAAACTACTGAAATTCGACACGCGCGCGCCGCCTTCAAAAAAGGCACATTGTCCCTTGCGGATTATGAAGCGGCAATGAAAAAAGAAATCGAATTGGTGGTGCGTGAGCAAGAAAAATTGGATTTAGa                                        <  1:659676/251‑1 (MQ=255)                                                                                                                                                                                                            tttGGCGAACTTGCCAAAAGGCGCGGATCAACGTAAATCCTCGTTTGCGGAACGTATCAAATTACAAAATGCCTGGTTAAATCTACCGTTATTGCCAACCACGAATATCGGTTCTTTCCCGCAAACTACTGAAATTCGACACGCGCGCGCCGCCTTCAAAAAAGGCACATTGTCCCTTGCGGATTATGAAGCGGCAATGAAAAAAGAAATCGAATTGGTGGTGCGTGAGCAAGAAAAATTGGATTTAGACg                                      >  1:422788/1‑251 (MQ=255)                                                                                                                                                                                                            tttGGCGAACTTGCCAAAAGGCGCGGATCAACGTAAATCCTCGTTTGCGGAACGTATCAAATTACAAAATGCCTGGTTAAATCTACCGTTATTGCCAACCACGAATATCGGTTCTTTCCCGCAAACTACTGAAATTCGACACGCGCGCGCCGCCTTCAAAAAAGGCACATTGTCCCTTGCGGATTATGAAGCGGCAATGAAAAAAGAAATCGAATTGGTGGTGCGTGAGCAAGAAAAATTGGATTTAGACg                                      <  1:117463/251‑1 (MQ=255)                                                                                                                                                                                                                    aCTTGCCAAAAGGCGCGGATCAACGTAAATCCTCGTTTGCGGAACGTATCAAATTACAAAATGCCTGGTTAAATCTACCGTTATTGCCAACCACGAATATCGGTTCTTTCCCGCAAACTACTGAAATTCGACACGCGCGCGCCGCCTTCAAAAAAGGCACATTGTCCCTTGCGGATTATGAAGCGGCAATGAAAAAAGAAATCGAATTGGTGGTGCGTGAGCAAGAAAAATTGGATTTAGACGTGCTGGtt                              <  1:274660/251‑1 (MQ=255)                                                                                                                                                                                                                     cTTGCCAAAAGGCGCGGATCAACGTAAATCCTCGTTTGCGGAACGTATCAAATTACAAAATGCCTGGTTAAATCTACCGTTATTGCCAACCACGAATATCGGTTCTTTCCCGCAAACTACTGAAATTCGACACGCGCGCGCCGCCTTCAAAAAAGGCACATTGTCCCTTGCGGATTATGAAGCGGCAATGAAAAAAGAAATCGAATTGGTGGTGCGTGAGCAAGAAAAATTGGATTTAGACGTGCTGGtt                              >  1:178292/1‑250 (MQ=255)                                                                                                                                                                                                                              aGGCGCGGATCAACGTAAATCCTCGTTTGCGGAACGTATCAAATTACAAAATGCCTGGTTAAATCTACCGTTATTGCCAACCACGAATATCGGTTCTTTCCCGCAAACTACTGAAATTCGACACGCGCGCGCCGCCTTCAAAAAAGGCACATTGTCCCTTGCGGATTATGAAGCGGCAATGAAAAAAGAAATCGAATTGGTGGTGCGTGAGCAAGAAAAATTGGATTTAGACGTGCTGGTTCACGGCGAAg                    >  1:90675/1‑251 (MQ=255)                                                                                                                                                                                                                                     gATCAACGTAAATCCTCGTTTGCGGAACGTATCAAATTACAAAATGCCTGGTTAAATCTACCGTTATTGCCAACCACGAATATCGGTTCTTTCCCGCAAACTACTGAAATTCGACACGCGCGCGCCGCCTTCAAAAAAGGCACATTGTCCCTTGCGGATTATGAAGCGGCAATGAAAAAAGAAATCGAATTGGTGGTGCGTGAGCAAGAAAAATTGGATTTAGACGTGCTGGTTCACGGCGAAGCGGAACg             >  1:78408/1‑251 (MQ=255)                                                                                                                                                                                                                                     gATCAACGTAAATCCTCGTTTGCGGAACGTATCAAATTACAAAATGCCTGGTTAAATCTACCGTTATTGCCAACCACGAATATCGGTTCTTTCCCGCAAACTACTGAAATTCGACACGCGCGCGCCGCCTTCAAAAAAGGCACATTGTCCCTTGCGGATTATGAAGCGGCAATGAAAAAAGAAATCGAATTGGTGGTGCGTGAGCAAGAAAAATTGGATTTAGACGTGCTGGTTCACGGCGAAGCGGAACg             >  1:539216/1‑251 (MQ=255)                                                                                                                                                                                                                                             tAAATCCTCGTTTGCGGAACGTATCAAATTACAAAATGCCTGGTTAAATCTACCGTTATTGCCAACCACGAATATCGGTTCTTTCCCGCAAACTACTGAAATTCGACACGCGCGCGCCGCCTTCAAAAAAGGCACATTGTCCCTTGCGGATTATGAAGCGGCAATGAAAAAAGAAATCGAATTGGTGGTGCGTGAGCAAGAAAAATTGGATTTAGACGTGCTGGTTCACGGCGAAGCGGAACGTAACGAc      <  1:636218/250‑1 (MQ=255)                                                                                                                                                                                                                                             tAAATCCTCGTTTGCGGAACGTATCAAATTACAAAATGCCTGGTTAAATCTACCGTTATTGCCAACCACGAATATCGGTTCTTTCCCGCAAACTACTGAAATTCGACACGCGCGCGCCGCCTTCAAAAAAGGCACATTGTCCCTTGCGGATTATGAAGCGGCAATGAAAAAAGAAATCGAATTGGTGGTGCGTGAGCAAGAAAAATTGGATTTAGACGTGCTGGTTCACGGCGAAGCGGAACGTAACGAc      <  1:163362/250‑1 (MQ=255)                                                                                                                                                                                                                                              aaaTCCTCGTTTGCGGAACGTATCAAATTACAAAATGCCTGGTTAAATCTACCGTTATTGCCAACCACGAATATCGGTTCTTTCCCGCAAACTACTGAAATTCGACACGCGCGCGCCGCCTTCAAAAAAGGCACATTGTCCCTTGCGGATTATGAAGCGGCAATGAAAAAAGAAATCGAATTGGTGGTGCGTGAGCAAGAAAAATTGGATTTAGACGTGCTGGTTCACGGCGAAGCGGAACGTAACGACAt    <  1:718250/251‑1 (MQ=255)                                                                                                                                                                                                                                                aTCCTCGTTTGCGGAACGTATCAAATTACAAAATGCCTGGTTAAATCTACCGTTATTGCCAACCACGAATATCGGTTCTTTCCCGCAAACTACTGAAATTCGACACGCGCGCGCCGCCTTCAAAAAAGGCACATTGTCCCTTGCGGATTATGAAGCGGCAATGAAAAAAGAAATCGAATTGGTGGTGCGTGAGCAAGAAAAATTGGAt                                             >  1:95654/1‑208 (MQ=255)                                                                                                                                                                                                                                                aTCCTCGTTTGCGGAACGTATCAAATTACAAAATGCCTGGTTAAATCTACCGTTATTGCCAACCACGAATATCGGTTCTTTCCCGCAAACTACTGAAATTCGACACGCGCGCGCCGCCTTCAAAAAAGGCACATTGTCCCTTGCGGATTATGAAGCGGCAATGAAAAAAGAAATCGAATTGGTGGTGCGTGAGCAAGAAAAATTGGATTTAGACGTGCTGGTTCACGGCGAAGCGGAACGTAACGACAtgg  >  1:421193/1‑251 (MQ=255)                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                       GTGGAAACACAGTTACAAGTAAACAAACCTAAACTTTACAGCTGGCTTGCATTCTGTTTACAAAAAATTCAAGAATTACGCGTCATTAAGACCGCACTTGAGCAAGGCAGAGCCGCCGTACAAGCGGAACTGGATGCCAGCCAAGTTGCCGCCGATGCCCGTGCGACCTCAAAAGATATTCATCGCCCTGAAGTGGCGGAACGTTTGGCGAACTTGCCAAAAGGCGCGGATCAACGTAAATCCCCGTTTGCGGAACGTATCAAATTACAAAATGCCTGGTTAAATCTACCGTTATTGCCAACCACGAATATCGGTTCTTTCCCGCAAACTACTGAAATTCGACACGCGCGCGCCGCCTTCAAAAAAGGCACATTGTCCCTTGCGGATTATGAAGCGGCAATGAAAAAAGAAATCGAATTGGTGGTGCGTGAGCAAGAAAAATTGGATTTAGACGTGCTGGTTCACGGCGAAGCGGAACGTAACGACATGG  >  1/1769136‑1769625

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑