Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,768,668	G→A	intergenic (+48710/‑26174)	unknown → / → unknown	tRNA‑Asn‑GTT/tRNA‑Leu‑CAA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,768,668	0	G	A	100.0%	211.4 / NA	78	intergenic (+48710/‑26174)	unknown/unknown	tRNA‑Asn‑GTT/tRNA‑Leu‑CAA
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (48/30);  total (48/30)

GATCAATATTTCCAACTGGCGCGCGGTAACAAAGCACAATTTGCCATTGAAATGACCAAATGGTTTGACACCAACTATCATTACCTCGTGCCGGAATTCCACGCGGATACCCAATTCAAAGCTAATCCGGCGCATTACGTGCAACAAATTCGCGAAGCCAAAGCCCTTGGCTTGAATGTTAAACCCACTATTGTTGGTCCATTAACGTTCTTGTGGTTAGGCAAAGAAAAAGGTGCGGCATTTGATCGCTTTGATTTATTAAATAAACTCATACCGGTTTATGTAGAAATCTTAAACGCATTGGCAGCAGAAGGCGTGGATTATATTCAAATTGACGAACCTGCTTTAACTGTCGATTTGCCGGCTAAATGGGTTGCGGCTTATAAAGACGTGTACGCCACATTAAGCACACAAGTAAACGCAAAATTACTGTTAGCCACTTATTTCGGTTCTGTCGCGGAACACGCTGCGTTATTAAAAGC  >  1/1768425‑1768906                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                               gATCAATATTTCCAACTGGCGCGCGGTAACAAAGCACAATTTGCCATTGAAATGACCAAATGGTTTGACACCAACTATCATTACCTCGTGCCGGAATTCCACGCGGATACCCAATTCAAAGCTAATCCGGCGCATTACGTGCAACAAATTCGCGAAGCCAAAGCCCTTGGCTTGAATGTTAAACCCACTATTGTTGGTCCATTAACGTTCTTGTGGTTAGGCAAAGAAAAAGGTGCGGCATTTAATCGCtt                                                                                                                                                                                                                                         >  1:873217/1‑251 (MQ=255) gATCAATATTTCCAACTGGCGCGCGGTAACAAAGCACAATTTGCCATTGAAATGACCAAATGGTTTGACACCAACTATCATTACCTCGTGCCGGAATTCCACGCGGATACCCAATTCAAAGCTAATCCGGCGCATTACGTGCAACAAATTCGCGAAGCCAAAGCCCTTGGCTTGAATGTTAAACCCACTATTGTTGGTCCATTAACGTTCTTGTGGTTAGGCAAAGAAAAAGGTGCGGCATTTAATCGCtt                                                                                                                                                                                                                                         >  1:668099/1‑251 (MQ=255)    cAATATTTCCAACTGGCGCGCGGTAACAAAGCACAATTTGCCATTGAAATGACCAAATGGTTTGACACCAACTATCATTACCTCGTGCCGGAATTCCACGCGGATACCCAATTCAAAGCTAATCCGGCGCATTACGTGCAACAAATTCGCGAAGCCAAAGCCCTTGGCTTGAATGTTAAACCCACTATTGTTGGTCCATTAACG‑TCTTGTGGTTAGGCAAAGAAAAAGGTGCGGCATTTAATCGCTTTGAt                                                                                                                                                                                                                                     >  1:610471/1‑251 (MQ=255)      atatTTCCAACTGGCGCGCGGTAACAAAGCACAATTTGCCATTGAAATGACCAAATGGTTTGACACCAACTATCATTACCTCGTGCCGGAATTCCACGCGGATACCCAATTCAAAGCTAATCCGGCGCATTACGTGCAACAAATTCGCGAAGCCAAAGCCCTTGGCTTGAATGTTAAACCCACTATTGTTGGTCCATTAACGTTCTTGTGGTTAGGCAAAGAAAAAGGTGCGGCATTTAATCGCTTTGAtt                                                                                                                                                                                                                                    <  1:545618/251‑1 (MQ=255)                cTGGCGCGCGGTAACAAAGCACAATTTGCCATTGAAATGACCAAATGGTTTGACACCAACTATCATTACCTCGTGCCGGAATTCCACGCGGATACCCAATTCAAAGCTAATCCGGCGCATTACGTGCAACAAATTCGCGAAGCCAAAGCCCTTGGCTTGAATGTTAAACCCACTATTGTTGGTCCATTAACGTTCTTGTGGTTAGGCAAAGAAAAAGGTGCGGCATTTAATCGCTTTGATTTATtaaataa                                                                                                                                                                                                                          >  1:780388/1‑251 (MQ=255)                    cgcgcgGTAACAAAGCACAATTTGCCATTGAAATGACCAAATGGTTTGACACCAACTATCATTACCTCGTGCCGGAATTCCACGCGGATACCCAATTCAAAGCTAATCCGGCGCATTACGTGCAACAAATTCGCGAAGCCAAAGCCCTTGGCTTGAATGTTAAACCCACTATTGTTGGTCCATTAACGTTCTTGTGGTTAGGCAAAGAAAAAGGTGCGGCATTTAATCGCTTTGATTTATTAAATAAACt                                                                                                                                                                                                                       <  1:4621/250‑1 (MQ=255)                                   cacaATTTGCCATTGAAATGACCAAATGGTTTGACACCAACTATCATTACCTCGTGCCGGAATTCCACGCGGATACCCAATTCAAAGCTAATCCGGCGCATTACGTGCAACAAATTCGCGAAGCCAAGGCCCTTGGCTTGAATGTTAAACCCTCTATTGTTGGTCCATTAACGTTCTTGTGGTTAGGCAAAGAAAAAGGTGCGGCATTTAATCGCTTTGATTTATTAAATAAACTCATACCGGTTTATGt                                                                                                                                                                                                        <  1:612866/250‑1 (MQ=255)                                   cacaATTTGCCATTGAAATGACCAAATGGTTTGACACCAACTATCATTACCTCGTGCCGGAATTCCACGCGGATACCCAATTCAAAGCTAATCCGGCGCATTACGTGCAACAAATTCGCGAAGCCAAAGCCCTTGGCTTGAATGTTAAACCCACTATTGTTGGTCCATTAACGTTCTTGTGGTTAGGCAAAGAAAAAGGTGCGGCATTTAATCGCTTTGATTTATTAAATAAACTCATACCGGTTTATGt                                                                                                                                                                                                        <  1:642854/250‑1 (MQ=255)                                   cacaATTTGCCATTGAAATGACCAAATGGTTTGACACCAACTATCATTACCTCGTGCCGGAATTCCACGCGGATACCCAATTCAAAGCTAATCCGGCGCATTACGTGCAACAAATTCGCGAAGCCAAAGCCCTTGGCTTGAATGTTAAACCCACTATTGTTGGTCCATTAACGTTCTTGTGGTTAGGCAAAGAAAAAGGTGCGGCATTTAATCGCTTTGATTTATTAAATAAACTCATACCGGTTTATGt                                                                                                                                                                                                        <  1:710883/250‑1 (MQ=255)                                               ttGAAATGACCAAATGGTTTGACACCAACTATCATTACCTCGTGCCGGAATTCCACGCGGATACCCAATTCAAAGCTAATCTGGCGCATTACGTGCAACAAATTCGCGAAGCCAAAGCCCTTGGCTTGAATGTTAAACCCACTATTGTTGGTCCATTAACGTTCTTGTGGTTAGGCAAAGAAAAAGGTGCGGCATTTAATCGCTTTGATTTATTAAATAAACTCATACCGGTTTATGTAGAAATCTTAAAc                                                                                                                                                                                           <  1:432999/251‑1 (MQ=255)                                               ttGAAATGACCAAATGGTTTGACACCAACTATCATTACCTCGTGCCGGAATTCCACGCGGATACCCAATTCAAAGCTAATCCGGCGCATTACGTGCAACAAATTCGCGAAGCCAAAGCCCTTGGCTTGAATGTTAAACCCACTATTGTTGGTCCATTAACGTTCTTGTGGTTAGGCAAAGAAAAAGGTGCGGCATTTAATCGCTTTGATTTATTAAATAAACTCATACCGGTTTATGTAGAAATCTTAAAc                                                                                                                                                                                           <  1:15001/251‑1 (MQ=255)                                                  aaaTGACCAAATGGTTTGACACCAACTATCATTACCTCGTGCCGGAATTCCACGCGGATACCCAATTCAAAGCTAATCCGGCGCATTACGTGCAACAAATTCGCGAAGCCAAAGCCCTTGGCTTGAATGTTAAACCCACTATTGTTGGTCCATTAACGTTCTTGTGGTTAGGCAAAGAAAAAGGTGCGGCATTTAATCGCTTTGATTTATTAAATAAACTCATACCGGTTTATGTAGAAATCTTAAACGc                                                                                                                                                                                         <  1:472863/250‑1 (MQ=255)                                                      gACCAAATGGTTTGACACCAACTATCATTACCTCGTGCCGGAATTCCACGCGGATACCCAATTCAAAGCTAATCCGGCGCATTACGTGCAACAAATTCGCGAAGCCAAAGCCCTTGGCTTGAATGTTAAACCCACTATTGTTGGTCCATTAACGTTCTTGTGGTTAGGCAAAGAAAAAGGTGCGGCATTTAATCGCTTTGATTTATTAAATAAACTCATACCGGTTTATGTAGAAATCTTAAACGCATTgg                                                                                                                                                                                    >  1:790637/1‑251 (MQ=255)                                                       aCCAAATGGTTTGACACCAACTATCATTACCTCGTGCCGGAATTCCACGCGGATACCCAATTCAAAGCTAATCCGGCGCATTACGTGCAACAAATTCGCGAAGCCAAAGCCCTTGGCTTGAATGTTAAACCCACTATTGTTGGTCCATTAACGTTCTTGTGGTTAGGCAAAGAAAAAGGTGCGGCATTTAATCGCTTTGATTTATTAAATAAACTCATACCGGTTTATGTAGaaa                                                                                                                                                                                                   >  1:849431/1‑235 (MQ=255)                                                           aaTGGTTTGACACCAACTATCATTACCTCGTGCCGGAATTCCACGCGGATACCCAATTCAAAGCTAATCCGGCGCATTACGTGCAACAAATTCGCGAAGCCAAAGCCCTTGGCTTGAATGTTAAACCCACTATTGTTGGTCCATTAACGTTCTTGTGGTTAGGCAAAGAAAAAGGTGCGGCATTTAATCGCTTTGATTTATTAAATAAACTCATACCGGTTTATGt                                                                                                                                                                                                        <  1:56846/226‑1 (MQ=255)                                                               gTTTGACACCAACTATCATTACCTCGTGCCGGAATTCCACGCGGATACCCAATTCAAAGCTAATCCGGCGCATTACGTGCAACAAATTCGCGAAGCCAAAGCCCTTGGCTTGAATGTTAAACCCACTATTGTTGGTCCATTAACGTTCTTGTGGTTAGGCAAAGAAAAAGGTGCGGCATTTAATCGCTTTGATTTATTAAATAAACTCATACCGGTTTATGTAGAAATCTTAAACGCATTGGCAGCAGAAg                                                                                                                                                                           >  1:278470/1‑251 (MQ=255)                                                                   gACACCAACTATCATTACCTCGTGCCGGAATTCCACGCGGATACCCAATTCAAAGCTAATCCGGCGCATTACGTGCAACAAATTCGCGAAGCCAAAGCCCTTGGCTTGAATGTTAAACCCACTATTGTTGGTCCATTAACGTTCTTGTGGTTAGGCAAAGAAAAAGGTGCGGCATTTAATCGCTTTGATTTATTAAATAAACTCATACCGGTTTATGTAGAAATCTTAAACGCATTGGCAGCAGAAGGCGt                                                                                                                                                                       >  1:522571/1‑251 (MQ=255)                                                                       ccAACTATCATTACCTCGTGCCGGAATTCCACGCGGATACCCAATTCAAAGCTAATCCGGCGCATTACGTGCAACAAATTCGCGAAGCCAAAGCCCTTGGCTTGAATGTTAAACCCACTATTGTTGGTCCATTAACGTTCTTGTGGTTAGGCAAAGAAAAAGGTGCGGCATTTAATCGCTTTGATTTATTAAATAAACTCATACCGGTTTATGTAGAAATCTTAAACGCATTGGCAGCAGAAGGCGTGGAt                                                                                                                                                                   <  1:681631/251‑1 (MQ=255)                                                                           cTATCATTACCTCGTGCCGGAATTCCACGCGGATACCCAATTCAAAGCTAATCCGGCGCATTACGTGCAACAAATTCGCGAAGCCAAAGCCCTTGGCTTGAATGTTAAACCCACTATTGTTGGTCCATTAACGTTCTTGTGGTTAGGCAAAGAAAAAGGTGCGGCATTTAATCGCTTTGATTTATTAAATAAACTCATACCGGTTTATGTAGAAATCTTAAACGCATTGGCAGCAGAAGGCGTGGATtata                                                                                                                                                               >  1:587453/1‑251 (MQ=255)                                                                             aTCATTACCTCGTGCCGGAATTCCACGCGGATACCCAATTCAAAGCTAATCCGGCGCATTACGTGCAACAAATTCGCGAAGCCAAAGCCCTTGGCTTGAATGTTAAACCCACTATTGTTGGTCCATTAACGTTCTTGTGGTTAGGCAAAGAAAAAGGTGCGGCATTTAATCGCTTTGATTTATTAAATAAACTCATACCGGTTTATGTAGAAATCTTAAACGCATTGGCAGCAGAAGGCGTGGATTATAtt                                                                                                                                                             >  1:398072/1‑251 (MQ=255)                                                                              tCATTACCTCGTGCCGGAATTCCACGCGGATACCCAATTCAAAGCTAATCCGGCGCATTACGTGCAACAAATTCGCGAAGCCAAAGCCCTTGGCTTGAATGTTAAACCCACTATTGTTGGTCCATTAACGTTCTTGTGGTTAGGCAAAGAAAAAGGTGCGGCATTTAATCGCTTTGATTTATTAAATAAACTCATACCGGTTTATGTAGAAATCTTAAACGCATTGGCAGCAGAAGGCGTGGATTATAtt                                                                                                                                                             >  1:692801/1‑250 (MQ=255)                                                                              tCATTACCTCGTGCCGGAATTCCACGCGGATACCCAATTCAAAGCTAATCCGGCGCATTACGTGCAACAAATTCGCGAAGCCAAAGCCCTTGGCTTGAATGTTAAACCCACTATTGTTGGTCCATTAACGTTCTTGTGGTTAGGCAAAGAAAAAGGTGCGGCATTTAATCGCTTTGATTTATTAAATAAACTCATACCGGTTTATGTAGAAATCTTAAACGCATTGGCAGCAGAAGGCGTGGATTATATTc                                                                                                                                                            <  1:167910/251‑1 (MQ=255)                                                                                          gCCGGAATTCCACGCGGATACCCAATTCAAAGCTAATCCGGCGCATTACGTGCAACAAATTCGCGAAGCCAAAGCCCTTGGCTTGAATGTTAAACCCACTATTGTTGGTCCATTAACGTTCTTGTGGTTAGGCAAAGAAAAAGGTGCGGCATTTAATCGCTTTGATTTATTAAATAAACTCATACCGGTTTATGTAGAAATCTTAAACGCATTGGCAGCAGAAGGCGTGGATTATATTc                                                                                                                                                            >  1:862898/1‑239 (MQ=255)                                                                                           ccGGAATTCCACGCGGATACCCAATTCAAAGCTAATCCGGCGCATTACGTGCAACAAATTCGCGAAGCCAAAGCCCTTGGCTTGAATGTTAAACCCACTATTGTTGGTCCATTAACGTTCTTGTGGTTAGGCAAAGAAAAAGGTGCGGCATTTAATCGCTTTGATTTATTAAATAAACTCATACCGGTTTATGTAGAAATCTTAAACGCATTGGCAGCAGAAGGCGTGGATTATATTCAAATTGACGAAc                                                                                                                                                >  1:327562/1‑250 (MQ=255)                                                                                                      cgcgGATACCCAATTCAAAGCTAATCCGGCGCATTACGTGCAACAAATTCGCGAAGCCAAAGCCCTTGGCTTGAATGTTAAACCCACTATTGTTGGTCCATTAACGTTCTTGTGGTTAGGCAAAGAAAAAGGTGCGGCATTTAATCGCTTTGATTTATTAAATAAACTCATACCGGTTTATGTAGAAATCTTAAACGCATTGGCAGCAGAAGGCGTGGATTATATTCAAATTGACGAACCTGCTTTaa                                                                                                                                       >  1:601303/1‑248 (MQ=255)                                                                                                          gATACCCAATTCAAAGCTAATCCGGCGCATTACGTGCAACAAATTCGCGAAGCCAAAGCCCTTGGCTTGAATGTTAAACCCACTATTGTTGGTCCATTAACGTTCTTGTGGTTAGGCAAAGAAAAAGGTGCGGCATTTAATCGCTTTGATTTATTAAATAAACTCATACCGGTTTATGTAGAAATCTTAAACGCATTGGCAGCAGAAGGCGTGGATTATATTCAAATTGACGAACCTGCTTTAACTGTCGa                                                                                                                                >  1:703876/1‑251 (MQ=255)                                                                                                             aCCCAATTCAAAGCTAATCCGGCGCATTACGTGCAACAAATTCGCGAAGCCAAAGCCCTTGGCTTGAATGTTAAACCCACTATTGTTGGTCCATTAACGTTCTTGTGGTTAGGCAAAGAAAAAGGTGCGGCATTTAATCGCTTTGATTTATTAAATAAACTCATACCGGTTTATGt                                                                                                                                                                                                        >  1:676412/1‑176 (MQ=255)                                                                                                             aCCCAATTCAAAGCTAATCCGGCGCATTACGTGCAACAAATTCGCGAAGCCAAAGCCCTTGGCTTGAATGTTAAACCCACTATTGTTGGTCCATTAACGTTCTTGTGGTTAGGCAAAGAAAAAGGTGCGGCATTTAATCGCTTTGATTTATTAAATAAACTCATACCGGTTTATGTAGAAATCTTAAACGCATTGGCAGCAGAAGGCGTGGATTATATTc                                                                                                                                                            <  1:269404/220‑1 (MQ=255)                                                                                                             aCCCAATTCAAAGCTAATCCGGCGCATTACGTGCAACAAATTCGCGAAGCCAAAGCCCTTGGCTTGAATGTTAAACCCACTATTGTTGGTCCATTAACGTTCTTGTGGTTAGGCAAAGAAAAAGGTGCGGCATTTAATCGCTTTGATTTATTAAATAAACTCATACCGGTTTATGTAGAAATCTTAAACGCATTGGCAGCAGAAGGCGTGGATTATATTCAAATTGACGAACCTGCTTTAACTGTCGAttt                                                                                                                             >  1:345573/1‑251 (MQ=255)                                                                                                             aCCCAATTCAAAGCTAATCCGGCGCATTACGTGCAACAAATTCGCGAAGCCAAAGCCCTTGGCTTGAATGTTAAACCCACTATTGTTGGTCCATTAACGTTCTTGTGGTTAGGCAAAGAAAAAGGTGCGGCATTTAATCGCTTTGATTTATTAAATAAACTCATACCGGTTTATGTAGAAATCTTAAACGCATTGGCAGCAGAAGGCGTGGATTATATTCAAATTGACGAACCTGCTTTAACTGTCGAttt                                                                                                                             >  1:79766/1‑251 (MQ=255)                                                                                                                   ttCAAAGCTAATCCGGCGCATTACGTGCAACAAATTCGCGAAGCCAAAGCCCTTGGCTTGAATGTTAAACCCACTATTGTTGGTCCATTAACGTTCTTGTGGTTAGGCAAAGAAAAAGGTGCGGCATTTAATCGCTTTGATTTATTAAATAAACTCATACCGGTTTATGTAGAAATCTTAAACGCATTGGCAGCAGAAGGCGTGGATTATATTCAAATTGACGAACCTGCTTTAACTGTCGATTTGCCgg                                                                                                                        >  1:169944/1‑250 (MQ=255)                                                                                                                        aGCTAATCCGGCGCATTACGTGCAACAAATTCGCGAAGCCAAAGCCCTTGGCTTGAATGTTAAACCCACTATTGTTGGTCCATTAACGTTCTTGTGGTTAGGCAAAGAAAAAGGTGCGGCATTTAATCGCTTTGATTTATTAAATAAACTCATACCGGTTTATGTAGAAATCTTAAACGCATTGGCAGCAGAAGGCGTGGATTATATTCAAATTGACGAACCTGCTTTAAc                                                                                                                                      <  1:394019/231‑1 (MQ=255)                                                                                                                        aGCTAATCCGGCGCATTACGTGCAACAAATTCGCGAAGCCAAAGCCCTTGGCTTGAATGTTAAACCCACTATTGTTGGTCCATTAACGTTCTTGTGGTTAGGCAAAGAAAAAGGTGCGGCATTTAATCGCTTTGATTTATTAAATAAACTCATACCGGTTTATGTAGAAATCTTAAACGCATTGGCAGCAGAAGGCGTGGATTATATTCAAATTGACGAACCTGCTTTAACTGTCGATTTGCCGGCTAAAt                                                                                                                  >  1:81138/1‑251 (MQ=255)                                                                                                                        aGCTAATCCGGCGCATTACGTGCAACAAATTCGCGAAGCCAAAGCCCTTGGCTTGAATGTTAAACCCACTATTGTTGGTCCATTAACGTTCTTGTGGTTAGGCAAAGAAAAAGGTGCGGCATTTAATCGCTTTGATTTATTAAATAAACTCATACCGGTTTATGTAGAAATCTTAAACGCATTGGCAGCAGAAGGCGTGGATTATATTCAAATTGACGAACCTGCTTTAACTGTCGATTTGCCGGCTAAAt                                                                                                                  >  1:20490/1‑251 (MQ=255)                                                                                                                          cTAATCCGGCGCATTACGTGCAACAAATTCGCGAAGCCAAAGCCCTTGGCTTGAATGTTAAACCCACTATTGTTGGTCCATTAACGTTCTTGTGGTTAGGCAAAGAAAAAGGTGCGGCATTTAATCGCTTTGATTTATTAAATAAACTCATACCGGTTTATGt                                                                                                                                                                                                        <  1:788211/163‑1 (MQ=255)                                                                                                                          cTAATCCGGCGCATTACGTGCAACAAATTCGCGAAGCCAAAGCCCTTGGCTTGAATGTTAAACCCACTATTGTTGGTCCATTAACGTTCTTGTGGTTAGGCAAAGAAAAAGGTGCGGCATTTAATCGCTTTGATTTATTAAATAAACTCATACCGGTTTATGTAGAAATCTTAAACGCATTGGCAGCAGAAGGCGTGGATTATATTCAAATTGACGAACCTGCTTTAACTGTCGATTTGCCGGCTAAATg                                                                                                                 <  1:575479/250‑1 (MQ=255)                                                                                                                                 ggCGCATTACGTGCAACAAATTCGCGAAGCCAAAGCCCTTGGCTTGAATGTTAAACCCACTATTGTTGGTCCATTAACGTTCTTGTGGTTAGGCAAAGAAAAAGGTGCGGCATTTAATCGCTTTGATTTATTAAATAAACTCATACCGGTTTATGTAGAAATCTTAAACGCATTGGCAGCAGAAGGCGTGGATTATATTCAAATTGACGAACCTGCTTTAACTGTCGATTTGCCGGCTAAATGGGTTGCgg                                                                                                         >  1:812639/1‑251 (MQ=255)                                                                                                                                     cATTACGTGCAACAAATTCGCGAAGCCAAAGCCCTTGGCTTGAATGTTAAACCCACTATTGTTGGTCCATTAACGTTCTTGTGGTTAGGCAAAGAAAAAGGTGCGGCATTTAATCGCTTTGATTTATTAAATAAACTCATACCGGTTTATGTAGAAATCTTAAACGCATTGGCAGCAGAAGGCGTGGATTATATTCAAATTGACGAACCTGCTTTAACTGTCGATTTGCCGGCTAAATGGGTTGCGGCTta                                                                                                     >  1:427464/1‑251 (MQ=255)                                                                                                                                      aTTACGTGCAACAAATTCGCGAAGCCAAAGCCCTTGGCTTGAATGTTAAACCCACTATTGTTGGTCCATTAACGTTCTTGTGGTTAGGCAAAGAAAAAGGCGCGGCATTTAATCGCTTTGATTTATTAAATAAACTCATACCGGTTTATGTAGAAATCTTAAACGCATTGGCAGCAGAAGGCGTGGATTATATTCAAATTGACGAACCTGCTTTAACTGTCGATTTGCCGGCTAAATGGGTTGCGGCTtat                                                                                                    >  1:371009/1‑251 (MQ=255)                                                                                                                                              caacaaATTCGCGAAGCCAAAGCCCTTGGCTTGAATGTTAAACCCACTATTGTTGGTCCATTAACGTTCTTGTGGTTAGGCAAAGAAAAAGGTGCGGCATTTAATCGCTTTGATTTATTAAATAAACTCATACCGGTTTATGTAGAAATCTTAAACGCATTGGCAGCAGAAGGCGTGGATTATATTCAAATTGACGAACCTGCTTTAACTGTCGATTTGCCGGCTAAATGGGTTGCGGCTTATAAAGACg                                                                                             <  1:69881/250‑1 (MQ=255)                                                                                                                                              caacaaATTCGCGAAGCCAAAGCCCTTGGCTTGAATGTTAAACCCACTATTGTTGGTCCATTAACGTTCTTGTGGTTAGGCAAAGAAAAAGGTGCGGCATTTAATCGCTTTGATTTATTAAATAAACTCATACCGGTTTATGTAGAAATCTTAAACGCATTGGCAGCAGAAGGCGTGGATTATATTCAAATTGACGAACCTGCTTTAACTGTCGATTTGCCGGCTAAATGGGTTGCGGCTTATAAAGACg                                                                                             <  1:209327/250‑1 (MQ=255)                                                                                                                                                    aTTCGCGAAGCCAAAGCCCTTGGCTTGAATGTTAAACCCACTATTGTTGGTCCATTAACGTTCTTGTGGTTAGGCAAAGAAAAAGGTGCGGCATTTAATCGCTTTGATTTATTAAATAAACTCATACCGGTTTATGTAGAAATCTTAAACGCATTGGCAGCAGAAGGCGTGGATTATATTCAAATTGACGAACCTGCTTTAACTGTCGATTTGCCGGCTAAATGGGTTGCGGCTTATAAAGACGTGTACGc                                                                                      <  1:782609/251‑1 (MQ=255)                                                                                                                                                             gCCAAAGCCCTTGGCTTGAATGTTAAACCCACTATTGTTGGTCCATTAACGTTCTTGTGGTTAGGCAAAGAAAAAGGTGCGGCATTTAATCGCTTTGATTTATTAAATAAACTCATACCGGTTTATGTAGAAATCTTAAACGCATTGGCAGCAGAAGGCGTGGATTATATTCAAATTGACGAACCTGCTTTAACTGTCGATTTGCCGGCTAAATGGGTTGCGGCTTATAAAGa                                                                                               >  1:73587/1‑233 (MQ=255)                                                                                                                                                             gCCAAAGCCCTTGGCTTGAATGTTAAACCCACTATTGTTGGTCCATTAACGTTCTTGTGGTTAGGCAAAGAAAAAGGCGCGGCATTTAATCGCTTTGATTTATTAAATAAACTCATACCGGTTTATGTAGAAATCTTAAACGCATTGGCAGCAGAAGGCGTGGATTATATTCAAATTGACGAACCTGCTTTAACTGTCGATTTGCCGGCTAAATGGGTTGCGGCTTATAAAGACGTGTACGCCACATTAAg                                                                             >  1:560285/1‑251 (MQ=255)                                                                                                                                                              ccAAAGCCCTTGGCTTGAATGTTAAACCCACTATTGTTGGTCCATTAACGTTCTTGTGGTTAGGCAAAGAAAAAGGTGCGGCATTTAATCGCTTTGATTTATTAAATAAACTCATACCGGTTTATGTAGAAATCTTAAACGCATTGGCAGCAGAAGGCGTGGATTATATTCAAATTGACGAACCTGCTTTAACTGTCGATTTGCCGGCTAAATGGGTTGCGGCTTATAAAGACGTGTACGCCACATTAAg                                                                             <  1:892985/250‑1 (MQ=255)                                                                                                                                                              ccAAAGCCCTTGGCTTGAATGTTAAACCCACTATTGTTGGTCCATTAACGTTCTTGTGGTTAGGCAAAGAAAAAGGTGCGGCATTTAATCGCTTTGATTTATTAAATAAACTCATACCGGTTTATGTAGAAATCTTAAACGCATTGGCAGCAGAAGGCGTGGATTATATTCAAATTGACGAACCTGCTTTAACTGTCGATTTGCCGGCTAAATGGGTTGCGGCTTATAAAGACGTGTACGCCACATTAAGc                                                                            <  1:519061/251‑1 (MQ=255)                                                                                                                                                              ccAAAGCCCTTGGCTTGAATGTTAAACCCACTATTGTTGGTCCATTAACGTTCTTGTGGTTAGGCAAAGAAAAAGGTGCGGCATTTAATCGCTTTGATTTATTAAATAAACTCATACCGGTTTATGTAGAAATCTTAAACGCATTGGCAGCAGAAGGCGTGGATTATATTCAAATTGACGAACCTGCTTTAACTGTCGATTTGCCGGCTAAATGGGTTGCGGCTTATAAAGACGTGTACGCCACATTAAGc                                                                            <  1:247277/251‑1 (MQ=255)                                                                                                                                                                   gCCCTTGGCTTGAATGTTAAACCCGCTATTGTTGGTCCATTAACGTTCTTGTGGTTAGGCAAAGAAAAAGGTGCGGCATTTAATCGCTTTGATTTATTAAATAAACTCATACCGGTTTATGTAGAAATCTTAAACGCATTGGCAGCAGAAGGCGTGGATTATATTCAAATTGACGAACCTGCTTTAACTGTCGATTTGCCGGCTAAATGGGTTGCGGCTTATAAAGACGTGTACGcc                                                                                     >  1:83573/1‑237 (MQ=255)                                                                                                                                                                   gCCCTTGGCTTGAATGTTAAACCCACTATTGTTGGTCCATTAACGTTCTTGTGGTTAGGCAAAGAAAAAGGTGCGGCATTTAATCGCTTTGATTTATTAAATAAACTc                                                                                                                                                                                                                      >  1:146774/1‑108 (MQ=255)                                                                                                                                                                     ccTTGGCTTGAATGTTAAACCCACTATTGTTGGTCCATTAACGTTCTTGTGGTTAGGCAAAGAAAAAGGTGCGGCATTTAATCGCTTTGATTTATTAAATAAACTCATACCGGTTTATGTAGAAATCTTAAACGCATTGGCAGCAGAAGGCGTGGATTATATTCAAATTGACGAACCTGCTTTAACTGTCGATTTGCCGGCTAAATGGGTTGCGGCTTATAAAGACGTGTACGCCACATTAATCACACAAg                                                                     <  1:644327/251‑1 (MQ=255)                                                                                                                                                                         ggCTTGAATGTTAAACCCACTATTGTTGGTCCATTAACGTTCTTGTGGTTAGGCAAAGAAAAAGGTGCGGCATTTAATCGCTTTGATTTATTAAATAAACTCATACCGGTTTATGTAGAAATCTTAAACGCATTGGCAGCAGAAGGCGTGGATTATATTCAAATTGACGAACCTGCtt                                                                                                                                          >  1:776704/1‑178 (MQ=255)                                                                                                                                                                                aTGTTAAACCCACTATTGTTGGTCCATTAACGTTCTTGTGGTTAGGCAAAGAAAAAGGTGCGGCATTTAATCGCTTTGATTTATTAAATAAACTCATACCGGTTTATGTAGAAATCTTAAACGCATTGGCAGCAGAAGGCGTGGATTATATTCAAATTGACGAACCTGCTTTAACTGTCGATTTGCCGGCTAAATGGGTTGCGGCTTATAAAGACGTGTACGCCACATTAAGCACACAAGTAAACGCaaaa                                                          >  1:621918/1‑251 (MQ=255)                                                                                                                                                                                aTGTTAAACCCACTATTGTTGGTCCATTAACGTTCTTGTGGTTAGGCAAAGAAAAAGGTGCGGCATTTAATCGCTTTGATTTATTAAATAAACTCATACCGGTTTATGTAGAAATCTTAAACGCATTGGCAGCAGAAGGCGTGGATTATATTCAAATTGACGAACCTGCTTTAACTGTCGATTTGCCGGCTAAATGGGTTGCGGCTTATAAAGACGTGTACGCCACATTAAGCACACAAGTAAACGCaaaa                                                          >  1:196333/1‑251 (MQ=255)                                                                                                                                                                                aTGTTAAACCCACTATTGTTGGTCCATTAACGTTCTTGTGGTTAGGCAAAGAAAAAGGTGCGGCATTTAATCGCTTTGATTTATTAAATAAACTCATACCGGTTTATGTAGAAATCTTAAACGCATTGGCAGCAGAAGGCGTGGATTATATTCAAATTGACGAACCTGCTTTAACTGTCGATTTGCCGGCTAAATGGGTTGCGGCTTATAAAGACGTGTACGCCACATTAAGCACACAAGTAAACGCaaaa                                                          >  1:772916/1‑251 (MQ=255)                                                                                                                                                                                       aCCCACTATTGTTGGTCCATTAACGTTCTTGTGGTTAGGCAAAGAAAAAGGTGCGGCATTTAATCGCTTTGATTTATTAAATAAACTCATACCGGTTTATGTAGAAATCTTAAACGCATTGGCAGCAGAAGGCGTGGATTATATTCAAATTGACGAACCTGCTTTAACTGTCGATTTGCCGGCTAAATGGGTTGCGGCTTATAAAGACGTGTACGCCACATTAAGCACACAAGTa                                                                   >  1:886382/1‑235 (MQ=255)                                                                                                                                                                                       aCCCACTATTGTTGGTCCATTAACGTTCTTGTGGTTAGGCAAAGAAAAAGGTGCGGCATTTAATCGCTTTGATTTATTAAATAAACTCATACCGGTTTATGTAGAAATCTTAAACGCATTGGCAGCAGAAGGCGTGGATTATATTCAAATTGACGAACCTGCTTTAACTGTCGATTTGCCGGCTAAATGGGTTGCGGCTTATAAAGACGTGTACGCCACATTAAGCACACAAGTAAACGCAAAATTa                                                       >  1:71176/1‑247 (MQ=255)                                                                                                                                                                                         ccACTATTGTTGGTCCATTAACGTTCTTGTGGTTAGGCAAAGAAAAAGGTGCGGCATTTAATCGCTTTGATTTATTAAATAAACTCATACCGGTTTATGTAGAAATCTTAAACGCATTGGCAGCAGAAGGCGTGGATTATATTCAAATTGACGAACCTGCTTTAACTGTCGATTTGCCGGCTAAATGGGTTGCGGCTTATAAAGACGTGTACGCCACATTAAGCACACAAGTAAACGCAAAATTACTGTTa                                                 >  1:260338/1‑251 (MQ=255)                                                                                                                                                                                            cTATTGTTGGTCCATTAACGTTCTTGTGGTTAGGCAAAGAAAAAGGTGCGGCATTTAATCGCTTTGATTTATTAAATAAACTCATACCGGTTTATGTAGAAATCTTAAACGCATTGGCAGCAGAAGGCGTGGATTATATTCAAATTGACGAACCTGCTTTAACTGTCGATTTGCCGGCTAAATGGGTTGCGGCTTATAAAGACGTGTACGcc                                                                                     >  1:434701/1‑212 (MQ=255)                                                                                                                                                                                            cTATTGTTGGTCCATTAACGTTCTTGTGGTTAGGCAAAGAAAAAGGTGCGGCATTTAATCGCTTTGATTTATTAAATAAACTCATACCGGTTTATGTAGAAATCTTAAACGCATTGGCAGCAGAAGGCGTGGATTATATTCAAATTGACGAACCTGCTTTAACTGTCGATTTGCCGGCTAAATGGGTTGCGGCTTATAAAGACGTGTACGCCACATTAAGCACACAAGTAAACGc                                                              >  1:256335/1‑235 (MQ=255)                                                                                                                                                                                              aTTGTTGGTCCATTAACGTTCTTGTGGTTAGGCAAAGAAAAAGGTGCGGCATTTAATCGCTTTGATTTATTAAATAAACTCATACCGGTTTATGTAGAAATCTTAAACGCATTGGCAGCAGAAGGCGTGGAt                                                                                                                                                                   <  1:707453/132‑1 (MQ=255)                                                                                                                                                                                               ttgttgGTCCATTAACGTTCTTGTGGTTAGGCAAAGAAAAAGGTGCGGCATTTAATCGCTTTGATTTATTAAATAAACTCATACCGGTTTATGTAGAAATCTTAAACGCATTGGCAGCAGAAGGCGTGGATTATATTCAAATTGACGAACCTGCTTTAACTGTCGATTTGCCGGCTAAATGGGTTGCGGCTTATAAAGACg                                                                                             <  1:113887/201‑1 (MQ=255)                                                                                                                                                                                               ttgttgGTCCATTAACGTTCTTGTGGTTAGGCAAAGAAAAAGGCGCGGCATTTAATCGCTTTGATTTATTAAATAACCTCATACCGGTTTATGTAGAAATCTTAAACGCATTGGCAGCAGAAGGCGTGGATTATATTCAAATTGACGAACCTGCTTTAACTGTCGATTTGCCGGCTAAATGGTCTGCGGCTTATAAAGACGTGTACGCCACATTAAGCACACAAGTAAACGCAAAATTACTGTTAGCCa                                             >  1:531316/1‑249 (MQ=255)                                                                                                                                                                                                     gTCCATTAACGTTCTTGTGGTTAGGCAAAGAAAAAGGTGCGGCATTTAATCGCTTTGATTTATTAAATAAACTCATACCGGTTTATGTAGAAATCTTAAACGCATTGGCAGCAGAAGGCGTGGATTATATTCAAATTGACGAACCTGCTTTAACTGTCGATTTGCCGGCTAAATGGGTTGCGGCTTATAAAGACGTGTACGCCACATTAAGCACACAAGTAAACGCAAAATTACTGTTAGCCACTTAttt                                      >  1:881167/1‑250 (MQ=255)                                                                                                                                                                                                         aTTAACGTTCTTGTGGTTAGGCAAAGAAAAAGGTGCGGCATTTAATCGCTTTGATTTATTAAATAAACTCATACCGGTTTATGTAGAAATCTTAAACGCATTGGCAGCAGAAGGCGTGGATTATATTCAAATTGACGAACCTGCTTTAACTGTCGATTTGCCGGCTAAATGGGTTGCgg                                                                                                         >  1:656058/1‑179 (MQ=255)                                                                                                                                                                                                             aCGTTCTTGTGGTTAGGCAAAGAAAAAGGTGCGGCATTTAATCGCTTTGATTTATTAAATAAACTCATACCGGTTTATGTAGAAATCTTAAACGCATTGGCAGCAGAAGGCGTGGATTATATTCAAATTGACGAACCTGCTTTAACTGTCGATTTGCCGGCTAAATGGGTTGCGGCTTATAAAGACGTGTACGCCACATTAAGCACACAAGTAAACGCAAAATTACTGTTAGCCACTTATTTCGGtt                                 >  1:557900/1‑247 (MQ=255)                                                                                                                                                                                                                ttCTTGTGGTTAGGCAAAGAAAAAGGTGCGGCATTTAATCGCTTTGATTTATTAAATAAACTCATACCGGTTTATGTAGAAATCTTAAACGCATTGGCAGCAGAAGGCGTGGATTATATTCAAATTGACGAACCTGCTTTAACTGTCGATTTGCCGGCTAAATGGGTTGCGGCTTATaa                                                                                                  >  1:861079/1‑179 (MQ=255)                                                                                                                                                                                                                ttCTTGTGGTTAGGCAAAGAAAAAGGTGCGGCATTTAATCGCTTTGATTTATTAAATAAACTCATACCGGTTTATGTAGAAATCTTAAACGCATTGGCAGCAGAAGGCGTGGATTATATTCAAATTGACGAACCTGCTTTAACTGTCGATTTGCCGGCTAAATGGGTTGCGGCTTATAAAGACGTGTACGCCACATTAAGc                                                                            >  1:870578/1‑201 (MQ=255)                                                                                                                                                                                                                  cTTGTGGTTAGGCAAAGAAAAAGGTGCGGCATTTAATCGCTTTGATTTATTAAATAAACTCATACCGGTTTATGTAGAAATCTTAAACGCATTGGCAGCAGAAGGCGTGGATTATATTCAAATTGACGAACCTGCTTTAACTGTCAATTTGCCGGCTAAATGGGTTGCGGCTTATAAAGACGTGTACGCCACATTAAGCACACAAGTAAACGCAAAATTACTGTTAGCCACTTATTTCGGTTCTGTCGCgg                        >  1:516517/1‑251 (MQ=255)                                                                                                                                                                                                                    tgtgGTTAGGCAAAGAAAAAGGTGCGGCATTTAATCGCTTTGATTTATTAAATAAACTCATACCGGTTTATGTAGAAATCTTAAACGCATTGGCAGCAGAAGGCGTGGATTATATTCAAATTGACGAACCTGCTTTAACTGTCGATTTGCCGGCTAAATGGGTTGCGGCTTATAAAGACGTGTACGCCACATTAAGCACACAAGTAAACGCAAAATTACTGTTAGCCACTTATTTCGGTTCTGTCGCGGaa                      <  1:852249/251‑1 (MQ=255)                                                                                                                                                                                                                      tgGTTAGGCAAAGAAAAAGGTGCGGCATTTAATCGCTTTGATTTATTAAATAAACTCATACCGGTTTATGTAGAAATCTTAAACGCATTGGCAGCAGAAGGCGTGGATTATATTCAAATTGACGAACCTGCTTTAACTGTCGATTTGCCGGCTAAATGGGTTGCGGCTTATAAAGACGTGTACGCCACATTAAGCACACAAGTAAACGCAAAATTACTGTTAGCCACTTATTTCGGTTCTGTcg                           <  1:549032/244‑1 (MQ=255)                                                                                                                                                                                                                       ggTTAGGCAAAGAAAAAGGTGCGGCATTTAATCGCTTTGATTTATTAAATAAACTCATACCGGTTTATGTAGAAATCTTAAACGCATTGGCAGCAGAAGGCGTGGATTATATTCAAATTGACGAACCTGCTTTAACTGTCGATTTGCCGGCTAAATGGGTTGCGGCTTATAAAGACGTGTACGCCACATTAAGc                                                                            <  1:415175/194‑1 (MQ=255)                                                                                                                                                                                                                              cAAAGAAAAAGGTGCGGCATTTAATCGCTTTGATTTATTAAATAAACTCATACCGGTTTATGTAGAAATCTTAAACGCATTGGCAGCAGAAGGCGTGGATTATATTCAAATTGACGAACCTGCTTTAACTGTCGATTTGCCGGCTAAATGGGTTGCGGCTTATAAAGACGTGTACGCCACATTAAGCACACAAGTAAACGCAAAATTACTGTTAGCCACTTATTTCGGTTCTGTCGCGGAACACGCTGCg             <  1:723611/250‑1 (MQ=255)                                                                                                                                                                                                                                aaGAAAAAGGTGCGGCATTTAATCGCTTTGATTTATTAAATAAACTCATACCGGTTTATGTAGAAATCTTAAACGCATTGGCAGCAGAAGGCGTGGATTATATTCAAATTGACGAACCTGCTTTAACTGTCGATTTGCCGGCTAAATGGGTTGCGGCTTATAAAGACGTGTACGcc                                                                                     >  1:138926/1‑176 (MQ=255)                                                                                                                                                                                                                                aaGAAAAAGGTGCGGCATTTAATCGCTTTGATTTATTAAATAAACTCATACCGGTTTATGTAGAAATCTTAAACGCATTGGCAGCAGAAGGCGTGGATTATATTCAAATTGACGAACCTGCTTTAACTGTCGATTTGCCGGCTAAATGGGTTGCGGCTTATAAAGACGTGTACGCCACATTAAGCACACAAGTAAACGCAAAATTACTGTTAGCCACTTATTTCGGTTCTGTCGCGGAACACGCTGCGtta          >  1:762656/1‑251 (MQ=255)                                                                                                                                                                                                                                  gAAAAAGGTGCGGCATTTAATCGCTTTGATTTATTAAATAAACTCATACCGGTTTATGTAGAAATCTTAAACGCATTGGCAGCAGAAGGCGTGGATTATATTCAAATTGACGAACCTGCTTTAACTGTCGATTTGCCGGCTAAATGGGTTGCGGCTTATaaa                                                                                                 <  1:486104/162‑1 (MQ=255)                                                                                                                                                                                                                                  gAAAAAGGTGCGGCATTTAATCGCTTTGATTTATTAAATAAACTCATACCGGTTTATGTAGAAATCTTAAACGCATTGGCAGCAGAAGGCGTGGATTATATTCAAATTGACGAACCTGCTTTAACTGTCGATTTGCCGGCTAAATGGGTTGCGGCTTATAAAGACGTGTACGCCACATTAAGCACACAAGTAAACGCAAAATTACTGTTAGCCACTTATTTCGGTTc                                >  1:237333/1‑227 (MQ=255)                                                                                                                                                                                                                                         gTGCGGCATTTAATCGCTTTGATTTATTAAATAAACTCATACCGGTTTATGTAGAAATCTTAAACGCATTGGCAGCAGAAGGCGTGGATTATATTCAAATTGACGAACCTGCTTTAACTGTCGATTTGCCGGCTAAATGGGTTGCGGCTTATAAAGACGTGTACGCCACATTAAGCACACAAGTAAACGCAAAATTACTGTTAGCCACTTATTTCGGTTCTGTCGCGGAACACGCTGCGTTATTAAAAGc  >  1:129029/1‑250 (MQ=255)                                                                                                                                                                                                                                              gCATTTAATCGCTTTGATTTATTAAATAAACTCATACCGGTTTATGTAGAAATCTTAAACGCATTGGCAGCAGAAGGCGTGGATTATATTCaaa                                                                                                                                                         <  1:457683/94‑1 (MQ=255)                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                               GATCAATATTTCCAACTGGCGCGCGGTAACAAAGCACAATTTGCCATTGAAATGACCAAATGGTTTGACACCAACTATCATTACCTCGTGCCGGAATTCCACGCGGATACCCAATTCAAAGCTAATCCGGCGCATTACGTGCAACAAATTCGCGAAGCCAAAGCCCTTGGCTTGAATGTTAAACCCACTATTGTTGGTCCATTAACGTTCTTGTGGTTAGGCAAAGAAAAAGGTGCGGCATTTGATCGCTTTGATTTATTAAATAAACTCATACCGGTTTATGTAGAAATCTTAAACGCATTGGCAGCAGAAGGCGTGGATTATATTCAAATTGACGAACCTGCTTTAACTGTCGATTTGCCGGCTAAATGGGTTGCGGCTTATAAAGACGTGTACGCCACATTAAGCACACAAGTAAACGCAAAATTACTGTTAGCCACTTATTTCGGTTCTGTCGCGGAACACGCTGCGTTATTAAAAGC  >  1/1768425‑1768906

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑