Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,768,132	T→C	intergenic (+48174/‑26710)	unknown → / → unknown	tRNA‑Asn‑GTT/tRNA‑Leu‑CAA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,768,132	0	T	C	100.0%	101.3 / NA	36	intergenic (+48174/‑26710)	unknown/unknown	tRNA‑Asn‑GTT/tRNA‑Leu‑CAA
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (25/11);  total (25/11)

AAAACGGCTCGCACTTTAACGTTACATAGAAGAAATCCATAAAATCCATAAAGAAATCGATGAAAAAGTGCGGTCGTTTTTAAAAGCGTTTTTTATGATTACCACCGCAAACTAATAAAATACAGGATCATTAATTTCTTTCATAAACTTTTAGAAAATTATTAATTTGCATAATACCCCCGTTTTCGTCATTCTAGCCCACGTATAGACGTTTAGCCGTCTAAATCATTTAATACATTGAGGAATTAGTATGACAACTTTTCATTTATCCGGCTTTCCGCGCGTGGGCGCAAAACGTGAACTTAAATTTGCACAAGAACGTTATTGGCGCGGTGAAATCGCAGAAGCCGATTTATTAGACATCGCCAAAGCCTTACGCGAAATCAACTGGAAACACCAAGCGGCAGCGAATGCGGATTACGTCGCCGTGGCGGATTTCACCTTCTACGATCACATTTTAGACTTGCAAGTGGCAACA  >  1/1767899‑1768376                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                                     aaaacgacccgtaCTTTAACGTTACATAGAAGAAATCCATAAAATCCATAAAGAAATCGATGAAAAAGTGCGGTCGTTTTTAAAAGCGTTTTTTATGATTACCACCGCAAACTAATAAAATACAGGATCATTAATTTCTTTCATAAACTTTTAGAAAATTATTAATTTGCATAATACCCCCGTTTTCGTCATTCTAGCCCACGTATAGACGTTTAGCCGTCTAAATCATTTAACACATTGAGGa                                                                                                                                                                                                                                            >  1:401275/13‑244 (MQ=255) aaaaCGACCCGCACTTTAACGTTACATAGAAGAAATCCATAAAATCCATAAAGAAATCGATGAAAAAGTGCGGTCGTTTTTAAAAGCGTTTTTTATGATTACCACCGCAAACTAATAAAATACAGGATCATTAATTTCTTTCATAAACTTTTAGAAAATTATTAATTTGCATAATACCCCCGTTTTCGTCATTCTAGCCCACGTATAGACGTTTAGCCGTCTAAATCATTTAACACATTGAGGAATTAGTa                                                                                                                                                                                                                                     <  1:218830/251‑1 (MQ=255)                             gaagaaATCCATAAAATCCATAAAGAAATCGATGAAAAAGTGCGGTCGTTTTTAAAAGCGTTTTTTATGATTACCACCGCAAACTAATAAAATACAGGATCATTAATTTCTTTCATAAACTTTTAGAAAATTATTAATTTGCATAATACCCCCGTTTTCGTCATTCTAGCCCACGTATAGACGTTTAGCCGTCTAAATCATTTAACACATTGAGGAATTAGTATGACAACTTTTCATTTATCCGGCTTTc                                                                                                                                                                                                          >  1:142558/1‑250 (MQ=255)                                                                           cGTTTTTAAAAGCGTTTTTTATGATTACCACCGCAAACTAATAAAATACAGGATCATTAATTTCTTTCATAAACTTTTAGAAAATTATTAATTTGCATAATACCCCCGTTTTCGTCATTCTAGCCCACGTATAGACGTTTAGCCGTCTAAATCATTTAACACATTGAGGAATTAGTATGACAACTTTTCATTTATCCGGCTTTCCGCGCGTGGGCGCAAAACGTGAACTTAAATTTGCACAAGAACGTTAt                                                                                                                                                           <  1:539566/251‑1 (MQ=255)                                                                                          tttttATGATTACCACCGCAAACTAATAAAATACAGGATCATTAATTTCTTTCATAAACTTTTAGAAAATTATTAATTTGCATAATACCCCCGTTTTCGTCATTCTAGCCCACGTATAGACGTTTAGCCGTCTAAATCATTTAACACATTGAGGAATTAGTATGACAACTTTTCATTTATCCGGCTTTCCGCGCGTGGGCGCAAAACGTGAACTTAAATTTGCACAAGAACGTTATTGGCGCGGTGAAAt                                                                                                                                             >  1:350790/1‑250 (MQ=255)                                                                                           ttttATGATTACCACCGCAAACTAATAAAATACAGGATCATTAATTTCTTTCATAAACTTTTAGAAAATTATTAATTTGCATAATACCCCCGTTTTCGTCATTCTAGCCCACGTATAGACGTTTAGCCGTCTAAATCATTTAACACATTGAGGAATTAGTATGACAACTTTTCATTTATCCGGCTTTCCGCGCGTGGGCGCAAAACGTGAACTTAAATTTGCACAAGAACGTTATTGGCGCGGTGAAATCg                                                                                                                                           >  1:369229/1‑251 (MQ=255)                                                                                                                                            ttcATAAACTTTTAGAAAATTATTAATTTGCATAATACCCCCGTTTTCGTCATTCTAGCCCACGTATAGACGTTTAGCCGTCTAAATCATTTAACACATTGAGGAATTAGTATGACAACTTTTCATTTATCCGGCTTTCCGCGCGTGGGCGCAAAACGTGAACTTAAATTTGCACAAGAACGTTATTGGCGCGGTGAAATCGCAGAAGCCGATTTATTAGACATCGCCAAAGCCTTACGCGAAATCaa                                                                                             >  1:584881/1‑248 (MQ=255)                                                                                                                                                    cTTTTAGAAAATTATTAATTTGCATAATACCCCCGTTTTCGTCATTCTAGCCCACGTATAGACGTTTAGCCGTCTAAATCATTTAACACATTGAGGAATTAGTATGACAACTTTTCATTTATCCGGCTTTCCGCGCGTGGGCGCAAAACGTGAACTTAAATTTGCACAAGAACGTTATTGGCGCGGTGAAATCGCAGAAGCCGATTTATTAGACATCGCCAAAGCCTTAcgc                                                                                                     >  1:396057/1‑232 (MQ=255)                                                                                                                                                               ttattaATTTGCATAATACCCCCGTTTTCGTCATTCTAGCCCACGTATAGACGTTTAGCCGTCTAAATCATTTAACACATTGAGGAATTAGTATGACAACTTTTCATTTATCCGGCTTTCCGCGCGTGGGCGCAAAACGTGAACTTAAATTTGCACAAGAACGTTATTGGCGCGGTGAAATCGCAGAAGCCGATTTATTAGACAt                                                                                                                     <  1:839882/205‑1 (MQ=255)                                                                                                                                                               ttattaATTTGCATAATACCCCCGTTTTCGTCATTCTAGCCCACGTATAGACGTTTAGCCGTCTAAATCATTTAACACATTGAGGAATTAGTATGACAACTTTTCATTTATCCGGCTTTCCGCGCGTGGGCGCAAAACGTGAACTTAAATTTGCACAAGAACGTTATTGGCGCGGTGAAATCGCAGAAGCCGATTTATTAGACATCGCCAAAGCCTTACGCGAAATCAACTGGAAACACCAAGCGGCAGCg                                                                       >  1:67893/1‑251 (MQ=255)                                                                                                                                                               ttattaATTTGCATAATACCCCCGTTTTCGTCATTCTAGCCCACGTATAGACGTTTAGCCGTCTAAATCATTTAACACATTGAGGAATTAGTATGACAACTTTTCATTTATCCGGCTTTCCGCGCGTGGGCGCAAAACGTGAACTTAAATTTGCACAAGAACGTTATTGGCGCGGTGAAATCGCAGAAGCCGATTTATTAGACATCGCCAAAGCCTTACGCGAAATCAACTGGAAACACCAAGCGGCAGCg                                                                       >  1:603875/1‑251 (MQ=255)                                                                                                                                                               ttattaATTTGCATAATACCCCCGTTTTCGTCATTCTAGCCCACGTATAGACGTTTAGCCGTCTAAATCATTTAACACATTGAGGAATTAGTATGACAACTTTTCATTTATCCGGCTTTCCGCGCGTGGGCGCAAAACGTGAACTTAAATTTGCACAAGAACGTTATTGGCGCGGTGAAATCGCAGAAGCCGATTTATTAGACATCGCCAAAGCCTTACGCGAAATCAACTGGAAACACCAAGCGGCAGCg                                                                       >  1:601666/1‑251 (MQ=255)                                                                                                                                                                         gCATAATACCCCCGTTTTCGTCATTCTAGCCCACGTATAGACGTTTAGCCGTCTAAATCATTTAACACATTGAGGAATTAGTATGACAACTTTTCATTTATCCGGCTTTCCGCGCGTGGGCGCAAAACGTGAACTTAAATTTGCACAAGAACGTTATTGGCGCGGTGAAATCGCAGa                                                                                                                                       <  1:763996/177‑1 (MQ=255)                                                                                                                                                                         gCATAATACCCCCGTTTTCGTCATTCTAGCCCACGTATAGACGTTTAGCCGTCTAAATCATTTAACACATTGAGGAATTAGTATGACAACTTTTCATTTATCCGGCTTTCCGCGCGTGGGCGCAAAACGTGAACTTAAATTTGCACAAGAACGTTATTGGCGCGGTGAAATCGCAGAAGCCGATTTATTAGACATCGCCAAAGCCTTACGCGAAATCAACTGGAAACACCAAGCGGCAGCGAATGCGGAtt                                                             >  1:213974/1‑251 (MQ=255)                                                                                                                                                                           ataataCCCCCGTTTTCGTCATTCTAGCCCACGTATAGACGTTTAGCCGTCTAAATCATTTAACACATTGAGGAATTAGTATGACAACTTTTCATTTATCCGGCTTTCCGCGCGTGGGCGCAAAACGTGAACTTAAATTTGCACAAGAACGTTATTGGCGCGGTGAAATCGCAGAAGCCGATTTATTAGACATCGCCAAAGCCTTACGCGAAATCAACTGGAAACACCAAGCGGCAGCGAATGCGGATTa                                                            >  1:146030/1‑250 (MQ=255)                                                                                                                                                                            taataCCCCCGTTTTCGTCATTCTAGCCCACGTATAGACGTTTAGCCGTCTAAATCATTTAACACATTGAGGAATTAGTATGACAACTTTTCATTTATCCGGCTTTCCGCGCGTGGGCGCAAAACGTGAACTTAAATTTGCACAAGaa                                                                                                                                                                 <  1:83446/148‑1 (MQ=255)                                                                                                                                                                            taataCCCCCGTTTTCGTCATTCTAGCCCACGTATAGACGTTTAGCCGTCTAAATCATTTAACACATTGAGGAATTAGTATGACAACTTTTCATTTATCCGGCTTTCCGCGCGTGGGCGCAAAACGTGAACTTAAATTTGCACAAGAACGTTATTGGCGCGGTGAAATCGCAGAAGCCGATTTATTAGAc                                                                                                                       <  1:813943/190‑1 (MQ=255)                                                                                                                                                                              ataCCCCCGTTTTCGTCATTCTAGCCCACGTATAGACGTTTAGCCGTCTAAATCATTTAACACATTGAGGAATTAGTATGACAACTTTTCATTTATCCGGCTTTCCGCGCGTGGGCGCAAAACGTGAACTTAAATTTGCACAAGAACGTTATTGGCGCGGTGAAATCGCAGAAGCCGATTTATTAGACATCGCCAAAGCCTTACGCGAAATCAACTGGAAACACCAAGCg                                                                             <  1:38645/230‑1 (MQ=255)                                                                                                                                                                                               aTTCTAGCCCACGTATAGACGTTTAGCCGTCTAAATCATTTAACACATTGAGGAATTAGTATGACAAc                                                                                                                                                                                                                              >  1:97629/1‑68 (MQ=255)                                                                                                                                                                                                     gCCCACGTATAGACGTTTAGCCGTCTAAATCATTTAACACATTGAGGAATTAGTATGACAACTTTTCATTTATCCGGCTTTCCGCGCGTGGGCGCAAAACGTGAACTTAAATTTGCACAAGAACGTTATTGGCGCGGTGAAATCGCAGAAGCCGATTTATTAGACATCGCCAAAGCCTTACGCGAAATCAACTGGAAACACCAAGCGGCAGCGAATGCGGATTACGTCGCCGTGGCGGATTTCACCTTCTa                                 >  1:801085/1‑251 (MQ=255)                                                                                                                                                                                                      cccACGTATAGACGTTTAGCCGTCTAAATCATTTAACACATTGAGGAATTAGTATGACAACTTTTCATTTATCCGGCTTTCCGCGCGTGGGCGCAAAACGTGAACTTAAATTTGCACAAGAACGTTATTGGCGCGGTGAAATCGCAGAAGCCGATTTATTAGACATCGCCAAAGCCTTACGCGAAATCAACTGGAAACACCAAGCGGCAGCGAATGCGGATTACGTCGCCGTGGCGGATTTCACCTTCTa                                 >  1:684404/1‑250 (MQ=255)                                                                                                                                                                                                          cGTATAGACGTTTAGCCGTCTAAATCATTTAACACATTGAGGAATTAGTATGACAACTTTTCATTTATCCGGCTTTCCGCGCGTGGGCGCAAAACGTGAACTTAAATTTGCACAAGAACGTTATTGGCGCGGTGAAATCGCAGAAGCCGATTTATTAGACATCGCCAAAGcc                                                                                                           >  1:781403/1‑172 (MQ=255)                                                                                                                                                                                                          cGTATAGACGTTTAGCCGTCTAAATCATTTAACACATTGAGGAATTAGTATGACAACTTTTCATTTATCCGGCTTTCCGCGCGTGGGCGCAAAACGTGAACTTAAATTTGCACAAGAACGTTATTGGCGCGGTGAAATCGCAGAAGCCGATTTATTAGACATCGCCAAAGCCTTACGCGAAATCAACTGGAAACACCAAGCGGCAGCGAATGCGGATTACGTCGCCGTGGCGGATTTCACCTTCTACGAt                             >  1:528669/1‑250 (MQ=255)                                                                                                                                                                                                          cGTATAGACGTTTAGCCGTCTAAATCATTTAACACATTGAGGAATTAGCATGACAACTTTTCATTTATCCGGCTTTCCGCGCGTGGGCGCAAAACGTGAACTTAAATTTGCACAAGAACGTTATTGGCGCGGCGAAATCGCAGAAGCCGATTTATTAGACATCGCCAAAGCCTTACGCGAAATCAACTGGAAACACCAAGCGGCAGCGAATGCGGATTACGTCGCCGTGGCGGATTTCACCTTCTACGAt                             >  1:317004/1‑250 (MQ=255)                                                                                                                                                                                                           gTATAGACGTTTAGCCGTCTAAATCATTTAACACATTGAGGAATTAGTATGACAACTTTTCATTTATCCGGCTTTCCGCGCGTGGGCGCAAAACGTGAACTTAAATTTGCACAAGAACGTTATTGGCGCGGTGAAATCGCAGAAGCCGATTTATTAGACATCGCCAAAGCCTTACGCGAAATCAACTGGAAACACCAAGCGGCAGCGAATGCGGATTACGTCGCCGTGGCGGATTTCACCTTCTACGATca                           >  1:837159/1‑251 (MQ=255)                                                                                                                                                                                                                 aCGTTTAGCCGTCTAAATCATTTAACACATTGAGGAATTAGTATGACAACTTTTCATTTATCCGGCTTTCCGCGCGTGGGCGCAAAACGTGAACTTAAATTTGCACAAGAACGTTATTGGCGCGGTGAAATCGCAGAAGCCGATTTATTAGACATCGCCAAAGcc                                                                                                           >  1:470259/1‑165 (MQ=255)                                                                                                                                                                                                                  cGTTTAGCCGTCTAAATCATTTAACACATTGAGGAATTAGTATGACAACTTTTCATTTATCCGGCTTTCCGCGCGTGGGCGCAAAACGTGAACTTAAATTTGCACAAGAACGTTATTGGCGCGGTGAAATCGCAGAAGCCGATTTATTAGACATCGCCAAAGCCTTACGCGAAATCAACTGGAAACACCAAGCGGCAGCGAATGCGGATTACGTCGCCGTGGCGGATTTCACCTTCTACGATCACATTTTa                    >  1:261488/1‑251 (MQ=255)                                                                                                                                                                                                                    tttAGCCGTCTAAATCATTTAACACATTGAGGAATTAGTATGACAACTTTTCATTTATCCGGCTTTCCGCGCGTGGGCGCAAAACGTGAACTTAAATTTGCACAAGAACGTTATTGGCGCGGTGAAATCGCAGAAGCCGATTTATTAGACATCGCCAAAGCCTTACGCGAAATCAACTGGAAACACCAAGCGGCAGCGAATGCGGATTACGTCGCCGTGGCGGATTTCACCTTCTACGATCACATTTTAGa                  <  1:292705/251‑1 (MQ=255)                                                                                                                                                                                                                     ttAGCCGTCTAAATCATTTAACACATTGAGGAATTAGTATGACAACTTTTCATTTATCCGGCTTTCCGCGCGTGGGCGCAAAACGTGAACTTAAATTTGCACAAGAACGTTATTGGCGCGGTGAAATCGCAGAAGCCGATTTATTAGACATCGCCAAAGCCTTACGCGAAATCAACTGGAAACACCAAGCGGCAGCGAATGCGGATTACGTCGCCGTGGCGGATTTCACCTTCTACGATCACATTTTAGAc                 <  1:324646/251‑1 (MQ=255)                                                                                                                                                                                                                           gTCTAAATCATTTAACACATTGAGGAATTAGTATGACAACTTTTCATTTATCCGGCTTTCCGCGCGTGGGCGCAAAACGTGAACTTAAATTTGCACAAGAACGTTATTGGCGCGGTGAAATCGCAGAAGCCGATTTATTAGAc                                                                                                                       <  1:475937/143‑1 (MQ=255)                                                                                                                                                                                                                           gTCTAAATCATTTAACACATTGAGGAATTAGTATGACAACTTTTCATTTATCCGGCTTTCCGCGCGTGGGCGCAAAACGTGAACTTAAATTTGCACAAGAACGTTATTGGCGCGGTGAAATCGCAGAAGCCGATTTATTAGACATCGCCAAAGCCTTACGCGAAATCAACTGGAAACACCAAGCGGCAGCGAATGCGGATTACGTCGCCGTGGCGGATTTCACCTTCTACGATCACATTTTAGACTTGCaa           >  1:437116/1‑251 (MQ=255)                                                                                                                                                                                                                                  tCATTTAACACATTGAGGAATTAGTATGACAACTTTTCATTTATCCGGCTTTCCGCGCGTGGGCGCAAAACGTGAACTTAAATTTGCACAAGAACGTTATTGGCGCGGTGAAATCGCAGAAGCCGATTTATTAGACATCGCCAAAGcc                                                                                                           >  1:440192/1‑148 (MQ=255)                                                                                                                                                                                                                                   cATTTAACACATTGAGGAATTAGTATGACAACTTTTCATTTATCCGGCTTTCCGCGCGTGGGCGCAAAACGTGAACTTAAATTTGCACAAGAACGTTATTGGCGCGGTGAAATCGCAGAAGCCGATTTATTAGACATCGCCAAAGCCTTACGCGAAATCAACTGGAAACACCAAGCGGCAGCGAATGCGGATTACGTCGCCGTGGCGGATTTCACCTTCTACGATCACATTTTAGACTTGCAAGTGGCaa    >  1:391720/1‑250 (MQ=255)                                                                                                                                                                                                                                    aTTTAACACATTGAGGAATTAGTATGACAACTTTTCATTTATCCGGCTTTCCGCGCGTGGGCGCAAAACGTGAACTTAAATTTGCACAAGAACGTTATTGGCGCGGTGAAATCGCAGAAGCCGATTTATTAGACATCGCCAAAGc                                                                                                            <  1:76963/145‑1 (MQ=255)                                                                                                                                                                                                                                    aTTTAACACATTGAGGAATTAGTATGACAACTTTTCATTTATCCGGCTTTCCGCGCGTGGGCGCAAAACGTGAACTTAAATTTGCACAAGAACGTTATTGGCGCGGTGAAATCGCAGAAGCCGATTTATTAGACATCGCCAAAGCCTTACGCGAAATCAACTGGAAACACCAAGCGGCAGCGAATGCGGATTACGTCGCCGTGGCGGATTTCACCTTCTACGATCACATTTTAGACTTGCAAGTGGCAACa  >  1:690767/1‑251 (MQ=255)                                                                                                                                                                                                                                     tttAACACATTGAGGAATTAGTATGACAACTTTTCATTTATCCGGCTTTCCGCGCGTGGGCGCAAAACGTGAACTTAAATTTGCACAAGAACGTTATTGGCGCGGTGAAATCGCAGAAGCCGATTTATTAGACATCGCCAAAGCCTTAcg                                                                                                      >  1:390415/1‑150 (MQ=255)                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                                     AAAACGGCTCGCACTTTAACGTTACATAGAAGAAATCCATAAAATCCATAAAGAAATCGATGAAAAAGTGCGGTCGTTTTTAAAAGCGTTTTTTATGATTACCACCGCAAACTAATAAAATACAGGATCATTAATTTCTTTCATAAACTTTTAGAAAATTATTAATTTGCATAATACCCCCGTTTTCGTCATTCTAGCCCACGTATAGACGTTTAGCCGTCTAAATCATTTAATACATTGAGGAATTAGTATGACAACTTTTCATTTATCCGGCTTTCCGCGCGTGGGCGCAAAACGTGAACTTAAATTTGCACAAGAACGTTATTGGCGCGGTGAAATCGCAGAAGCCGATTTATTAGACATCGCCAAAGCCTTACGCGAAATCAACTGGAAACACCAAGCGGCAGCGAATGCGGATTACGTCGCCGTGGCGGATTTCACCTTCTACGATCACATTTTAGACTTGCAAGTGGCAACA  >  1/1767899‑1768376

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑