Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,760,403	T→C	intergenic (+40445/‑34439)	unknown → / → unknown	tRNA‑Asn‑GTT/tRNA‑Leu‑CAA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,760,403	0	T	C	100.0%	111.8 / NA	40	intergenic (+40445/‑34439)	unknown/unknown	tRNA‑Asn‑GTT/tRNA‑Leu‑CAA
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (20/20);  total (20/20)

CAAACGACGCGCCCCATAGCGCAACCCTTCCCGCAATAATTTTAATTTACGTTTTTCAACGGGAAACATTTCATGAAATGTCGGAAAATGGAACGTTGCCACCATAAACCCCATAAAAATCGATTCAATAAATTAAGCGCCAGATTATAGCAAAAAGCCTTGTTATTTTAAATGCGCCCATGCGCCGTAAAAACACGAGGAAAAACCACCGCACTTAATCTGTCACTTCACTAAAACATGAACTCAATTAATTAACTAAAAAGCTACCCTGGATATTTCGCTACGGAAGGTAGTCAAAGTTACAAAATTTTGTATCAAATATTTTATAAATTTATTCTTTACATTAAACCAAACAAAAATATAATGCGAATCGTTTTTATCTATGGCCAGAATCTGAGGAAATACTATGTATAAAAAAACCAAGATCGCATTTTTCATTTGTACGGCGCTGTATGCACAGCATGTTCT  >  1/1760160‑1760627                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                 caaacGACGCGCCCCATAGCGCAACCCTTCCCGCAATAATTTTAATTTACGTTTTTCAACGGGAAACATTTCATGAAATGTTGGAAAATGGAACGTTGCCACCATAAACCCCATAAAAATCGATTCAATAAATTAAGCGCCAGATTATAGCAAAAAGCCTTGTTATTTTAAATGCGCCCATGCGCCGTAAAAACACGAGGAAAAACCACCGCACTTAATCTGTCACTTCACTAAAACATGAACCCaattaa                                                                                                                                                                                                                           <  1:441788/251‑1 (MQ=255)   aacGACGCGCCCCATAGCGCAACCCTTCCCGCAATAATTTTAATTTACGTTTTTCAACGGGAAACATTTCATGAAATGTCGGAAAATGGAACGTTGCCACCATAAACCCCATAAAAATCGATTCAATAAATTAAGCGCCAGATTATAGCAAAAAGCCTTGTTATTTTAAATGCGCCCATGCGCCGTAAAAACACGAGGAAAAACCACCGCACTTAATCTGTCACTTCACTAAAACATGAACCCaattaatt                                                                                                                                                                                                                         <  1:318621/251‑1 (MQ=255)              ccATAGCGCAACCCTTCCCGCAATAATTTTAATTTACGTTTTTCAACGGGAAACATTTCATGAAATGTCGGAAAATGGAACGTTGCCACCATAAACCCCATAAAAATCGATTCAATAAATTAAGCGCCAGATTATAGCAAAAAGCCTTGTTATTTTAAATGCGCCCATGCGCCGTAAAAACACGAGGAAAAACCACCGCACTTAATCTGTCACTTCACTAAAACATGAACCCaattaat                                                                                                                                                                                                                          >  1:623789/1‑239 (MQ=255)                   gcgcAACCCTTCCCGCAATAATTTTAATTTACGTTTTTCAACGGGAAACATTTCATGAAATGTCGGAAAATGGAACGTTGCCACCATAAACCCCATAAAAATCGATTCAATAAATTAAGCGCCAGATTATAGCAAAAAGCCTTGTTATTTTAAATGCGCCCATGCGCCGTAAAAACACGAGGAAAAACCACCGCACTTAATCTGTCACTTCACTAAAACATGAACCCAATTAATTAAc                                                                                                                                                                                                                      >  1:437903/1‑238 (MQ=255)                    cgcAACCCTTCCCGCAATAATTTTAATTTACGTTTTTCAACGGGAAACATTTCATGAAATGTCGGAAAATGGAACGTTGCCACCATAAACCCCATAAAAATCGATTCAATAAATTAAGCGCCAGATTATAGCAAAAAGCCTTGTTATTTTAAATGCGCCCATGCGCCGTAAAAACACGAGGAAAAACCACCGCACTTAATCTGTCACTTCACTAAAACATGAACCCAATTAATTAACTAAAAAGCTAccc                                                                                                                                                                                                         <  1:890185/250‑1 (MQ=255)                    cgcAACCCTTCCCGCAATAATTTTAATTTACGTTTTTCAACGGGAAACATTTCATGAAATGTCGGAAAATGGAACGTTGCCACCATAAACCCCATAAAAATCGATTCAATAAATTAAGCGCCAGATTATAGCAAAAAGCCTTGTTATTTTAAATGCGCCCATGCGCCGTAAAAACACGAGGAAAAACCACCGCACTTAATCTGTCACTTCACTAAAACATGAACCCAATTAATTAACTAAAAAGCTAccc                                                                                                                                                                                                         <  1:352473/250‑1 (MQ=255)                      cAACCCTTCCCGCAATAATTTTAATTTACGTTTTTCAACGGGAAACATTTCATGAAATGTCGGAAAATGGAACGTTGCCACCATAAACCCCATAAAAATCGATTCAATAAATTAAGCGCCAGATTATAGCAAAAAGCCTTGTTATTTTAAATGCGCCCATGCGCCGTAAAAACACGAGGAAAAACCACCGCACTTAATCTGTCACTTCACTAAAACATGAACCCaattaatt                                                                                                                                                                                                                         <  1:824625/232‑1 (MQ=255)                              cccGCAATAATTTTAATTTACGTTTTTCAACGGGAAACATTTCATGAAATGTCGGAAAATGGAACGTTGCCACCATAAACCCCATAAAAATCGATTCAATAAATTAAGCGCCAGATTATAGCAAAAAGCCTTGTTATTTTAAATGCGCCCATGCGCCGTAAAAACACGAGGAAAAACCACCGCACTTAATCTGTCACTTCACTAAAACATGAACCCAATTAATTAACTAAAAAGCTACCCTGGATATTTCg                                                                                                                                                                                              <  1:566204/251‑1 (MQ=255)                                    ataatTTTAATTTACGTTTTTCAACGGGAAACATTTCATGAAATGTCGGAAAATGGAACGTTGCCACCATAAACCCCATAAAAATCGATTCAATAAATTAAGCGCCAGATTATAGCAAAAAGCCTTGTTATTTTAAATGCGCCCATGCGCCGTAAAAACACGAGGAAAAACCACCGCACTTAATCTGTCACTTCACTAAAACATGAACCCAATTAATTAACTAAAAAGCTACCCTGGATATTTCGCTACgg                                                                                                                                                                                        <  1:362795/251‑1 (MQ=255)                                         tttaatttACGTTTTTCAACGGGAAACATTTCATGAAATGTCGGAAAATGGAACGTTGCCACCATAAACCCCATAAAAATCGATTCAATAAATTAAGCGCCAGATTATAGCAAAAAGCCTTGTTATTTTAAATGCGCCCATGCGCCGTAAAAACACGAGGAAAAACCACCGCACTTAATCTGTCACTTCACTAAAACATGAACCCAATTAATTAACTAAAAAGCTACCCTGGATATTTCGCTACGGAAGGt                                                                                                                                                                                   <  1:506304/251‑1 (MQ=255)                                         tttaatttACGTTTTTCAACGGGAAACATTTCATGAAATGTCGGAAAATGGAACGTTGCCACCATAAACCCCATAAAAATCGATTCAATAAATTAAGCGCCAGATTATAGCAAAAAGCCTTGTTATTTTAAATGCGCCCATGCGCCGTAAAAACACGAGGAAAAACCACCGCACTTAATCTGTCACTTCACTAAAACATGAACCCAATTAATTAACTAAAAAGCTACCCTGGATATTTCGCTACGGAAGGt                                                                                                                                                                                   <  1:803077/251‑1 (MQ=255)                                                                 aaCATTTCATGAAATGTCGGAAAATGGAACGTTGCCACCATAAACCCCATAAAAATCGATTCAATAAATTAAGCGCCAGATTATAGCAAAAAGCCTTGTTATTTTAAATGCGCCCATGCGCCGTAAAAACACGAGGAAAAACCACCGCACTTAATCTGTCACTTCACTAAAACATGAACCCAATTAATTAACTAAAAAGCTACCCTGGATATTTCGCTACGGAAGGTAGTCAAAGTTACAAAATTTTGTAt                                                                                                                                                           <  1:614903/251‑1 (MQ=255)                                                                   cATTTCATGAAATGTCGGAAAATGGAACGTTGCCACCATAAACCCCATAAAAATCGATTCAATAAATTAAGCGCCAGATTATAGCAAAAAGCCTTGTTATTTTAAATGCGCCCATGCGCCGTAAAAACACGAGGAAAAACCACCGCACTTAATCTGTCACTTCACTAAAACATGAACCCAATTAATTAACTAAAAAGCTACCCTGGATATTTCGCTACGGAAGGTAGTCAAAGTTACAAAATTTTGTATCa                                                                                                                                                         >  1:557373/1‑251 (MQ=255)                                                                                           gAACGTTGCCACCATAAACCCCATAAAAATCGATTCAATAAATTAAGCGCCAGATTATAGCAAAAAGCCTTGTTATTTTAAATGCGCCCATGCGCCGTAAAAACACGAGGAAAAACCACCGCACTTAATCTGTCACTTCACTAAAACATGAACCCAATTAATTAACTAAAAAGCTACCCTGGATATTTCGCTACGGAAGGTAGTCAAAGTTACAAAATTTTGTATCAAATATTTTATAAATTTATTc                                                                                                                                     >  1:398376/1‑247 (MQ=255)                                                                                                   ccaccaTAAACCCCATAAAAATCGATTCAATAAATTAAGCGCCAGATTATAGCAAAAAGCCTTGTTATTTTAAATGCGCCCATGCGCCGTAAAAACACGAGGAAAAACCACCGCACTTAATCTGTCACTTCACTAAAACATGAACCCAATTAATTAACTAAAAAGCTACCCTGGATATTTCGCTACGGAAGGTAGTCAAAGTTACAAAATTTTGTATCAAATATTTTATAAATTTATTCTTTACATTAAAc                                                                                                                         <  1:597895/251‑1 (MQ=255)                                                                                                      ccaTAAACCCCATAAAAATCGATTCAATAAATTAAGCGCCAGATTATAGCAAAAAGCCTTGTTATTTTAAATGCGCCCATGCGCCGTAAAAACACGAGGAAAAACCACCGCACTTAATCTGTCACTTCACTAAAACATGAACCCAATTAATTAACTAAAAAGc                                                                                                                                                                                                              <  1:786557/163‑1 (MQ=255)                                                                                                      ccaTAAACCCCATAAAAATCGATTCAATAAATTAAGCGCCAGATTATAGCAAAAAGCCTTGTTATTTTAAATGCGCCCATGCGCCGTAAAAACACGAGGAAAAACCACCGCACTTAATCTGTCACTTCACTAAAACATGAACCCAATTAATTAACTAAAAAGCTACCCTGGATATTTCGCTACGGAAGGTAGTCAAAGTTACAAAATTTTGTATCAAATATTTTATAAATTTATTCTTTACATTAAACcaa                                                                                                                      >  1:783110/1‑251 (MQ=255)                                                                                                      ccaTAAACCCCATAAAAATCGATTCAATAAATTAAGCGCCAGATTATAGCAAAAAGCCTTGTTATTTTAAATGCGCCCATGCGCCGTAAAAACACGAGGAAAAACCACCGCACTTAATCTGTCACTTCACTAAAACATGAACCCAATTAATTAACTAAAAAGCTACCCTGGATATTTCGCTACGGAAGGTAGTCAAAGTTACAAAATTTTGTATCAAATATTTTATAAATTTATTCTTTACATTAAACcaa                                                                                                                      >  1:366226/1‑251 (MQ=255)                                                                                                       caTAAACCCCATAAAAATCGATTCAATAAATTAAGCGCCAGATTATAGCAAAAAGCCTTGTTATTTTAAATGCGCCCATGCGCCGTAAAAACACGAGGAAAAACCACCGCACTTAATCTGTCACTTCACTAAAACATGAACCCAATTAATTAACTAAAAAGCTACCCTGGATATTTCGCTACGGAAGGTAGTCAAAGTTACAAAATTTTGTATCAAATATTTTATAAATTTATTCTTTACATTAAACcaaa                                                                                                                     >  1:595500/1‑251 (MQ=255)                                                                                                                cATAAAAATCGATTCAATAAATTAAGCGCCAGATTATAGCAAAAAGCCTTGTTATTTTAAATGCGCCCATGCGCCGTAAAAACACGAGGAAAAACCACCGCACTTAATCTGTCACTTCACTAAAACATGAACCCAATTAATTAACTAAAAAGCTACCCTGGATATTTCGCTACGGAAGGTAGTCAAAGTTACAAAATTTTGTATCAAATATTTTATAAATTTATTCTTTACATTAAACCAAACAAAAatat                                                                                                            >  1:161748/1‑251 (MQ=255)                                                                                                                  tAAAAATCGATTCAATAAATTAAGCGCCAGATTATAGCAAAAAGCCTTGTTATTTTAAATGCGCCCATGCGCCGTAAAAACACGAGGAAAAACCACCGCACTTAATCTGTCACTTCACTAAAACATGAACCCAATTAATTAACTAAAAAGCTACCCTGGATATTTCGCTACGGAAGGTAGTCAAAGTTACAAAATTTTGTATCAAATATTTTATAAATTTATTCTTTACATTAAACCAAACAAAAATATaa                                                                                                          <  1:274563/251‑1 (MQ=255)                                                                                                                                aTAAATTAAGCGCCAGATTATAGCAAAAAGCCTTGTTATTTTAAATGCGCCCATGCGCCGTAAAAACACGAGGAAAAACCACCGCACTTAATCTGTCACTTCACTAAAACATGAACCCaattaa                                                                                                                                                                                                                           <  1:68056/124‑1 (MQ=255)                                                                                                                                                                      ttttAAATGCGCCCATGCGCCGTAAAAACACGAGGAAAAACCACCGCACTTAATCTGTCACTTCACTAAAACATGAACCCAATTAATTAACTAAAAAGCTACCCTGGATATTTCGCTACGGAAGGTAGTCAAAGTTACAAAATTTTGTATCAAATATTTTATAAATTTATTCTTTACATTAAACCAAACAAAAATATAATGCGAATCGTTTTTATCTATGGCCAGAATCTGAGGAAATACTATGTATaaaa                                                      >  1:106369/1‑251 (MQ=255)                                                                                                                                                                                 cccATGCGCCGTAAAAACACGAGGAAAAACCACCGCACTTAATCTGTCACTTCACTAAAACATGAACCCAATTAATTAACTAAAAAGCTACCCTGGATATTTCGCTACGGAAGGTAGTCAAAGTTACAAAATTTTGTATCAAATATTTTATAAATTTATTCTTTACATTAAACCAAACAAAAATATAATGCGAATCGTTTTTATCTATGGCCAGAATCTGAGGAAATACTATGTATAAAAAAACCAAGAt                                            >  1:280008/1‑250 (MQ=255)                                                                                                                                                                                           gTAAAAACACGAGGAAAAACCACCGCACTTAATCTGTCACTTCACTAAAACATGAACCCAATTAATTAACTAAAAAGCTACCCTGGATATTTCGCTACGGAAGGTAGTCAAAGTTACAAAATTTTGTATCAAATATTTTATAAATTTATTCTTTACATTAAACCAAACAAAAATATAATGCGAATCGTTTTTATCTATGGCCAGAATCTGAGGAAATACTATGTATAAAAAAACCAAGATCGCATTTTTCa                                 >  1:296154/1‑251 (MQ=255)                                                                                                                                                                                                       ggAAAAACCACCGCACTTAATCTGTCACTTCACTAAAACATGAACCCAATTAATTAACTAAAAAGCTACCCTGGATATTTCGCTACGGAAGGTAGTCAAAGTTACAAAATTTTGTATCAAATATTTTATAAATTTATTCTTTACATTAAACCAAACAAAAATATAATGCGAATCGTTTTTATc                                                                                         >  1:373598/1‑183 (MQ=255)                                                                                                                                                                                                        gAAAAACCACCGCACTTAATCTGTCACTTCACTAAAACATGAACCCAATTAATTAACTAAAAAGCTAccc                                                                                                                                                                                                         >  1:135730/1‑70 (MQ=255)                                                                                                                                                                                                                    cACTTAATCTGTCACTTCACTAAAACATGAACCCAATTAATTAACTAAAAAGCTACCCTGGATATTTCGCTACGGAAGGTAGTCAAAGTTACAAAATTTTGTATCAAATATTTTATAAATTTATTCTTTACATTAAACCAAACAAAAATATAATGCGAATCGTTTTTATCTATGGCCAGAATCTGAGGAAATACTATGTATAAAAAAACCAAGATCGCATTTTTCATTTGTACGGCGCTGTATGCACAGc         <  1:424468/250‑1 (MQ=255)                                                                                                                                                                                                                      cTTAATCTGTCACTTCACTAAAACATGAACCCAATTAATTAACTAAAAAGCTACCCTGGATATTTCGCTACGGAAGGTAGTCAAAGTTACAAAATTTTGTATCAAATATTTTATAAATTTATTCTTTACATTAAACCAAACAAAAATATAATGCGAAt                                                                                                   >  1:827655/1‑158 (MQ=255)                                                                                                                                                                                                                        tAATCTGTCACTTCACTAAAACATGAACCCAATTAATTAACTAAAAAGCTACCCTGGATATTTCGCTACGGAAGGTAGTCAAAGTTACAAAATTTTGTATCAAATATTTTATAAATTTATTCTTTACATTAAACCAAACAAAAATATAATGCGAATCGTTTTTATCTATGGCCAGAATCTGAGGAAATACTATGTATAAAAAAACCAAGATCGCATTTTTCATTTGTAc                          <  1:126765/229‑1 (MQ=255)                                                                                                                                                                                                                          aTCTGTCACTTCACTAAAACATGAACCCAATTAATTAACTAAAAAGCTACCCTGGATATTTCGCTACGGAAGGTAGTCAAAGTTACAAAATTTTGTATCAAATATTTTATAAATTTATTCTTTACATTAAACCAAACAAAAATATAATGCGAATCGTTTTTATCTATTTCCAGAATc                                                                            >  1:436297/1‑177 (MQ=255)                                                                                                                                                                                                                          aTCTGTCACTTCACTAAAACATGAACCCAATTAATTAACTAAAAAGCTACCCTGGATATTTCGCTACGGAAGGTAGTCAAAGTTACAAAATTTTGTATCAAATATTTTATAAATTTATTCTTTACATTAAACCAAACAAAAATATAATGCGAATCGTTTTTATCTATGGCCAGAATCTGAgg                                                                       <  1:16358/182‑1 (MQ=255)                                                                                                                                                                                                                          aTCTGTCACTTCACTAAAACATGAACCCAATTAATTAACTAAAAAGCTACCCTGGATATTTCGCTACGGAAGGTAGTCAAAGTTACAAAATTTTGTATCAAATATTTTATAAATTTATTCTTTACATTAAACCAAACAAAAATATAATGCGAATCGTTTTTATCTATGGCCAGAATCTGAGGAAATACTATGTATAAAAAAACCAAGATCGCATTTTTCATTTGTACGGCGCTGTATGCACAGCATGTTCt  <  1:172510/251‑1 (MQ=255)                                                                                                                                                                                                                              gTCACTTCACTAAAACATGAACCCAATTAATTAACTAAAAAGCTACCCTGGATATTTCGCTACGGAAGGTAGTCAAAGTTACAAAATTTTGTATCAAATATTTTATAAATTTATTCTTTACATTAAACCAAACAAAAATATAATGCGAATCGTTTTTATCTATGGCCAGAATCTGAGGAAATACTATGTATAAAAAAACCAAGATCGCATTTTTCATTTGTACGGCGCTGTAt                >  1:403628/1‑233 (MQ=255)                                                                                                                                                                                                                                cacttcactAAAACATGAACCCAATTAATTAACTAAAAAGCTACCCTGGATATTTCGCTACGGAAGGTAGTCAAAGTTACAAAATTTTGTATCAAATATTTTATAAATTTATTCTTTACATTAAACCAAACAAAAATATAATGCGAATCGTTTTTATCTATGGCCAGAATCTGAGGAAATACTATGTATAAAAAAACCAAGATCGCATTTTTCATTTGTACGGCGCTGTATGcac            >  1:233718/1‑235 (MQ=255)                                                                                                                                                                                                                                cacttcactAAAACATGAACCCAATTAATTAACTAAAAAGCTACCCTGGATATTTCGCTACGGAAGGTAGTCAAAGTTACAAAATTTTGTATCAAATATTTTATAAATTTATTCTTTACATTAAACCAAACAAAAATATAATGCGAATCGTTTTTATCTATGGCCAGAATCTGAGGAAATACTATGTATAAAAAAACCAAGATCGCATTTTTCATTTGTACGGCGCTGTATGcac            >  1:227447/1‑235 (MQ=255)                                                                                                                                                                                                                                  cttcactAAAACATGAACCCAATTAATTAACTAAAAAGCTACCCTGGATATTTCGCTACGGAAGGTAGTCAAAGTTACAAAATTTTGTATCAAATATTTTATAAATTTATTCTTTACATTAAACCAAACAAAAATATaa                                                                                                          >  1:149908/1‑139 (MQ=255)                                                                                                                                                                                                                                  cttcactAAAACATGAACCCAATTAATTAACTAAAAAGCTACCCTGGATATTTCGCTACGGAAGGTAGTCAAAGTTACAAAATTTTGTATCAAATATTTTATAAATTTATTCTTTACATTAAACCAAACAAAAATATAATGCGAATCGTTTTTATCTATGGCCAGAATc                                                                            <  1:66471/169‑1 (MQ=255)                                                                                                                                                                                                                                    tcactAAAACATGAACCCAATTAATTAACTAAAAAGCTACCCTGGATATTTCGCTACGGAAGGTAGTCAAAGTTACAAAATTTTGTATCAAATATTTTATAAATTTATTCTTTACATTAAACCAAACAAAAATATAATGCGAATCGTTTTTATCTATGGCCAGAATCTGAGGAAATAc                                                                 <  1:766313/178‑1 (MQ=255)                                                                                                                                                                                                                                     cactAAAACATGAACCCAATTAATTAACTAAAAAGCTACCCTGGATATTTCGCTACGGAAGGTAGTCAAAGTTACAAAATTTTGTATCAAATATTTTATAAATTTATTCTTTACATTAAACCAAACAAAAATATAATGCGAATCGTTTTTATCTATGGCCAGAATCTGAGGAAATACTATGTATAAAAAAACCAAGATCGCATTTTTCATTTGTACGgcgc                     >  1:119334/1‑221 (MQ=255)                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                 CAAACGACGCGCCCCATAGCGCAACCCTTCCCGCAATAATTTTAATTTACGTTTTTCAACGGGAAACATTTCATGAAATGTCGGAAAATGGAACGTTGCCACCATAAACCCCATAAAAATCGATTCAATAAATTAAGCGCCAGATTATAGCAAAAAGCCTTGTTATTTTAAATGCGCCCATGCGCCGTAAAAACACGAGGAAAAACCACCGCACTTAATCTGTCACTTCACTAAAACATGAACTCAATTAATTAACTAAAAAGCTACCCTGGATATTTCGCTACGGAAGGTAGTCAAAGTTACAAAATTTTGTATCAAATATTTTATAAATTTATTCTTTACATTAAACCAAACAAAAATATAATGCGAATCGTTTTTATCTATGGCCAGAATCTGAGGAAATACTATGTATAAAAAAACCAAGATCGCATTTTTCATTTGTACGGCGCTGTATGCACAGCATGTTCT  >  1/1760160‑1760627

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑