Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,751,858	A→C	intergenic (+31900/‑42984)	unknown → / → unknown	tRNA‑Asn‑GTT/tRNA‑Leu‑CAA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,751,858	0	A	C	100.0%	228.2 / NA	83	intergenic (+31900/‑42984)	unknown/unknown	tRNA‑Asn‑GTT/tRNA‑Leu‑CAA
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (28/55);  total (28/55)

AATCGAAACCGATGAAAAAGGCCGTGAAAGCATCATCGTGAACGAAATTCCGTATCAAGTGAACAAAGCGAAACTCATCGAAAAAATTGCCGATTTCGTCAAAGACAAAAAAATTGAGGGGATCAGCGGGTTGCGCGATGAATCCGACAAAGACGGTATGCGCATCGTCATCGAAATCAAACGTGATGCGGTCGGCGAAGTGGTGTTAAATAACCTGTATGCGTTGACACAAATGCAAGTCACTTTAGGGATTAATATTGTCGCACTTGACCACGGTCAGCCCAAACTATTGAATTTAAAACAGCTCATTGAGGCGTTCGTATTACACCGCCGCGAAGTGGTGACCCGTCGTACGATTTTTGAATTACGCAAAGCCCGTGAACGTACGCACATCTTAGAAGGCTTGGCGATTGCCTTGGCAAACGTCGATCCGATTATCGAATTAATCCGCAAAGCACCAAATCCGGAAAACGCCAAACGTGAAT  >  1/1751612‑1752096                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                               aaTCGAAACCGATGAAAAAGGCCGTGAAAGCATCATCGTGAACGAAATTCCGTATCAAGTGAACAAAGCGAAACTCATCGAAAAAATTGCCGATTTCGTCAAAGACAAAAAAATTGAGGGGATCAGCGGGTTGCGCGATGAATCCGACAAAGACGGTATGCGCATCGTCATCGAAATCAAACGTGATGCGGTCGGCGAAGTGGTGTTAAATAACCTGTATGCGTTGACACAAATGCAAGTCACTTTCGGGa                                                                                                                                                                                                                                            <  1:17437/251‑1 (MQ=255)       aaCCGATGAAAAAGGCCGTGAAAGCATCATCGTGAACGAAATTCCGTATCAAGTGAACAAAGCGAAACTCATCGAAAAAATTGCCGATTTCGTCAAAGACAAAAAAATTGAGGGGATCAGCGGGTTGCGCGATGAATCCGACAAAGACGGTATGCGCATCGTCATCGAAATCAAACGTGATGCGGTCGGCGAAGTGGTGTTAAATAACCTGTATGCGTTGACACAAATGCAAGTCACTTTCGGGATTAata                                                                                                                                                                                                                                      <  1:607889/251‑1 (MQ=255)        aCCGATGAAAAAGGCCGTGAAAGCATCATCGTGAACGAAATTCCGTATCAAGTGAACAAAGCGAAACTCATCGAAAAAATTGCCGATTTCGTCAAAGACAAAAAAATTGAGGGGATCAGCGGGTTGCGCGATGAATCCGACAAAGACGGTATGCGCATCGTCATCGAAATCAAACGTGATGCGGTCGGCGAAGTGGTGTTAAATAACCTGTATGCGTTGACACAAATGCAAGTCACTTTCGGGATTAatat                                                                                                                                                                                                                                     <  1:457262/251‑1 (MQ=255)        aCCGATGAAAAAGGCCGTGAAAGCATCATCGTGAACGAAATTCCGTATCAAGTGAACAAAGCGAAACTCATCGAAAAAATTGCCGATTTCGTCAAAGACAAAAAAATTGAGGGGATCAGCGGGTTGCGCGATGAATCCGACAAAGACGGTATGCGCATCGTCATCGAAATCAAACGTGATGCGGTCGGCGAAGTGGTGTTAAATAACCTGTATGCGTTGACACAAATGCAAGTCACTTTCGGGATTAatat                                                                                                                                                                                                                                     <  1:48819/251‑1 (MQ=255)                aaaaGGCCGTGAAAGCATCATCGTGAACGAAATTCCGTATCAAGTGAACAAAGCGAAACTCATCGAAAAAATTGCCGATTTCGTCAAAGACAAAAAAATTGAGGGGATCAGCGGGTTGCGCGATGAATCCGACAAAGACGGTATGCGCATCGTCATCGAAATCAAACGTGATGCGGTCGGCGAAGTGGTGTTAAATAACCTGTATGCGTTGACACAAATGCAAGTCACTTTCGGGATTAATATTGTCGCAc                                                                                                                                                                                                                             <  1:358680/251‑1 (MQ=255)                      ccGTGAAAGCATCATCGTGAACGAAATTCCGTATCAAGTGAACAAAGCGAAACTCATCGAAAAAATTGCCGATTTCGTCAAAGACAAAAAAATTGAGGGGATCAGCGGGTTGCGCGATGAATCCGACAAAGACGGTATGCGCATCGTCATCGAAATCAAACGTGATGCGGTCGGCGAAGTGGTGTTAAATAACCTGTATGCGTTGACACAAATGCAAGTCACTTTCGGGATTAATATTGTCGCACTTGAcc                                                                                                                                                                                                                       <  1:611295/251‑1 (MQ=255)                       cGTGAAAGCATCATCGTGAACGAAATTCCGTATCAAGTGAACAAAGCGAAACTCATCGAAAAAATTGCCGATTTCGTCAAAGACAAAAAAATTGAGGGGATCAGCGGGTTGCGCGATGAATCCGACAAAGACGGTATGCGCATCGTCATCGAAATCAAACGTGATGCGGTCGGCGAAGTGGTGTTAAATAACCTGTATGCGTTGACACAAATGCAAGTCACTTTCGGGATTAATATTGTCGCACTTGACCa                                                                                                                                                                                                                      <  1:554331/251‑1 (MQ=255)                         tGAAAGCATCATCGTGAACGAAATTCCGTATCAAGTGAACAAAGCGAAACTCATCGAAAAAATTGCCGATTTCGTCAAAGACAAAAAAATTGAGGGGATCAGCGGGTTGCGCGATGAATCCGACAAAGACGGTATGCGCATCGTCATCGAAATCAAACGTGATGCGGTCGGCGAAGTGGTGTTAAATAACCTGTATGCGTTGACACAAATGCAAGTCACTTTCGGGATTAATATTGTCGCACTTGACCAc                                                                                                                                                                                                                     <  1:753807/250‑1 (MQ=255)                          gAAAGCATCATCGTGAACGAAATTCCGTATCAAGTGAACAAAGCGAAACTCATCGAAAAAATTGCCGATTTCGTCAAAGACAAAAAAATTGAGGGGATCAGCGGGTTGCGCGATGAATCCGACAAAGACGGTATGCGCATCGTCATCGAAATCAAACGTGATGCGGTCGGCGAAGTGGTGTTAAATAACCTGTATGCGTTGACACAAATGCAAGTCACTTTCGGGATTAATATTGTCg                                                                                                                                                                                                                                <  1:118656/238‑1 (MQ=255)                                     cGTGAACGAAATTCCGTATCAAGTGAACAAAGCGAAACTCATCGAAAAAATTGCCGATTTCGTCAAAGACAAAAAAATTGAGGGGATCAGCGGGTTGCGCGATGAATCCGACAAAGACGGTATGCGCATCGTCATCGAAATCAAACGTGATGCGGTCGGCGAAGTGGTGTTAAATAACCTGTATGCGTTGACACAAATGCAAGTCACTTTCGGGATTAATATTGTCGCACTTGACCACGGTCAGCCCaaa                                                                                                                                                                                                         >  1:339530/1‑250 (MQ=255)                                        gAACGAAATTCCGTATCAAGTGAACAAAGCGAAACTCATCGAAAAAATTGCCGATTTCGTCAAAGACAAAAAAATTGAGGGGATCAGCGGGTTGCGCGATGAATCCGACAAAGACGGTATGCGCATCGTCATCGAAATCAAACGTGATGCGGTCGGCGAAGTGGTGTTAGATAACCTGTATGCGTTGACACAAATGCAAGTCACTTTCGGGAt                                                                                                                                                                                                                                           >  1:423160/1‑213 (MQ=255)                                          aCGAAATTCCGTATCAAGTGAACAAAGCGAAACTCATCGAAAAAATTGCCGATTTCGTCAAAGACAAAAAAATTGAGGGGATCAGCGGGTTGCGCGATGAATCCGACAAAGACGGTATGCGCATCGTCATCGAAATCAAACGTGATGCGGTCGGCGAAGTGGTGTTAAATAACCTGTATGCGTTGACACAAATGCAAGTCACTTTCGGGATTAATATTGTCGCACTTGACCACGGTCAGCCCAAACTATTg                                                                                                                                                                                                   <  1:188314/251‑1 (MQ=255)                                               aTTCCGTATCAAGTGAACAAAGCGAAACTCATCGAAAAAATTGCCGATTTCGTCAAAGACAAAGAAATTGAGGGGATCAGCGGGTTGCGCGATGAATCCGACAAAGACGGTATGCGCATCGTCATCGAAATCAAACGTGATGCGGTCGGCGAAGTGGTGTTAAATAACCTGTATGCGTTGACACAAATGCAAGTCACTTTCGGGATTAATATTGTCGCACTTGACCACGGTCAGCCCAAACTATTGAAttt                                                                                                                                                                                              >  1:597785/1‑251 (MQ=255)                                                  ccGTATCAAGTGAACAAAGCGAAACTCATCGAAAAAATTGCCGATTTCGTCAAAGACAAAAAAATTGAGGGGATCAGCGGGTTGCGCGATGAATCCGACAAAGACGGTATGCGCATCGTCATCGAAATCAAACGTGATGCGGTCGGCGAAGTGGTGTTAAATAACCTGTATGCGTTGACACAAATGCAAGTCACTTTCGGGATTAATATTGTCGCACTTGACCACGGTCAGCCCAAACTATTGAATTTaaa                                                                                                                                                                                           >  1:537160/1‑251 (MQ=255)                                                     tATCAAGTGAACAAAGCGAAACTCATCGAAAAAATTGCCGATTTCGTCAAAGACAAAAAAATTGAGGGGATCAGCGGGTTGCGCGATGAATCCGACAAAGACGGTATGCGCATCGTCATCGAAATCAAACGTGATGCGGTCGGCGAAGTGGTGTTAAATAACCTGTATGCGTTGACACAAATGCAAGTCACTTTCGGGATTAATATTGTCGCACTTGACCACGGTCAGCCCAAACTATTGAATTTAAAAc                                                                                                                                                                                         <  1:58865/250‑1 (MQ=255)                                                            tGAACAAAGCGAAACTCATCGAAAAAATTGCCGATTTCGTCAAAGACAAAAAAATTGAGGGGATCAGCGGGTTGCGCGATGAATCCGACAAAGACGGTATGCGCATCGTCATCGAAATCAAACGTGATGCGGTCGGCGAAGTGGTGTTAAATAACCTGTATGCGTTGACACAAATGCAAGTCACTTTCGGGATTAATATTGTCGCACTTGACCACGGTCAGCCCAAACTATTGAATTTAAAACAGCTCAtt                                                                                                                                                                                 <  1:343646/251‑1 (MQ=255)                                                             gAACAAAGCGAAACTCATCGAGAAAATTGCCGATTTCGTCAAAGACAAAAAAATTGAGGGGATCAGCGGGTTGCGCGATGAATCCGACAAAGACGGTATGCGCATCGTCATCGAAATCAAACGTGATGCGGTCGGCGAAGTGGTGTTAAATAACCTGTATGCGTTGACACAAATGCAAGTCACTTTCGGGATTAATATTGTCGCACTTGACCACGGTCAGCCCAAACTATTGAATTTAAAAc                                                                                                                                                                                         <  1:129119/242‑1 (MQ=255)                                                                  aaGCGAAACTCATCGAAAAAATTGCCGATTTCGTCAAAGACAAAAAAATTGAGGGGATCAGCGGGTTGCGCGATGAATCTGACAAAGACGGTATGCGCATCGTCATCGAAATCAAACGTGATGCGGTCGGCGAAGTGGTGTTAAATAACCTGTATGCGTTGACACAAATGCAAGTCACTTTCGGGATTAATATTGTCGCACTTGACCACGGTCAGCCCAAACTATTGAATTTAAAACAGCTCATTGAgg                                                                                                                                                                             <  1:437549/249‑1 (MQ=255)                                                                  aaGCGAAACTCATCGAAAAAATTGCCGATTTCGTCAAAGACAAAAAAATTGAGGGGATCAGCGGGTTGCGCGATGAATCCGACAAAGACGGTATGCGCATCGTCATCGAAATCAAACGTGATGCGGTCGGCGAAGTGGTGTTAAATAACCTGTATGCGTTGACACAAATGCAAGTCACTTTCGGGATTAATATTGTCGCACTTGACCACGGTCAGCCCAAACTATTGAATTTAAAACAGCTCATTGAGGCg                                                                                                                                                                           <  1:462205/251‑1 (MQ=255)                                                                   aGCGAAACTCATCGAAAAAATTGCCGATTTCGTCAAAGACAAAAAAATTGAGGGGATCAGCGGGTTGCGCGATGAATCCGACAAAGACGGTATGCGCATCGTCATCGAAATCAAACGTGATGCGGTCGGCGAAGTGGTGTTAAATAACCTGTATGCGTTGACACAAATGCAAGTCACTTTCGGGATTAATATTGTCGCACTTGACCACGGTCAGCCCAAACTATTGAAt                                                                                                                                                                                                <  1:248880/229‑1 (MQ=255)                                                                       aaaCTCATCGAAAAAATTGCCGATTTCGTCAAAGACAAAAAAATTGAGGGGATCAGCGGGTTGCGCGATGAATCCGACAAAGACGGTATGCGCATCGTCATCGAAATCAAACGTGATGCGGTCGGCGAAGTGGTGTTAAATAACCTGTATGCGTTGACACAAATGCAAGTCACTTTCGGGATTAATATTGTCGCACTTGACCACGGTCAGCCCAAACTATTGAATTTAAAACAGCTCATTGAGGCGTTCGt                                                                                                                                                                      <  1:643290/251‑1 (MQ=255)                                                                          cTCATCGAAAAAATTGCCGATTTCGTCAAAGACAAAAAAATTGAGGGGATCAGCGGGTTGCGCGATGAATCCGACAAAGACGGTATGCGCATCGTCATCGAAATCAAACGTGATGCGGTCGGCGAAGTGGTGTTAAATAACCTGTATGCGTTGACACAAATGCAAGTCACTTTCGGGATTAATATTGTCGCACTTGACCACGGTCAGCCCAAACTATTGAATTTAAAACAGCTCATTGAGGCGTTCGTAtt                                                                                                                                                                   <  1:540146/251‑1 (MQ=255)                                                                            cATCGAAAAAATTGCCGATTTCGTCAAAGACAAAAAAATTGAGGGGATCAGCGGGTTGCGCGATGAATCCGACAAAGACGGTATGCGCATCGTCATCGAAATCAAACGTGATGCGGTCGGCGAAGTGGTGTTAAATAACCTGTATGCGTTGACACAAATGCAAGTCACTTTCGGGATTAATATTGTCGCACTTGACCACGGTCAGCCCAAACTATTGAATTTAAAACAGCTCATTGAGGCGTTCGTATTac                                                                                                                                                                 <  1:824068/251‑1 (MQ=255)                                                                              tCGAAAAAATTGCCGATTTCGTCAAAGACAAAAAAATTGAGGGGATCAGCGGGTTGCGCGATGAATCCGACAAAGACGGTATGCGCATCGTCATCGAAATCAAACGTGATGCGGTCGGCGAAGTGGTGTTAAATAACCTGTATGCGTTGACACAAATGCAAGTCACTTTCGGGATTAATATTGTCGCACTTGTCCACGGTCAGCCCAAACTATTGAATTTAAAACAGCTCATTGAGGCGTTCGTATTacac                                                                                                                                                               <  1:90408/251‑1 (MQ=255)                                                                              tCGAAAAAATTGCCGATTTCGTCAAAGACAAAAAAATTGAGGGGATCAGCGGGTTGCGCGATGAATCCGACAAAGACGGTATGCGCATCGTCATCGAAATCAAACGTGATGCGGTCGGCGAAGTGGTGTTAAATAACCTGTATGCGTTGACACAAATGCAAGTCACTTTCGGGATTAATATTGTCGCACTTGACCACGGTCAGCCCAAACTATTGAATTTAAAACAGCTCATTGAGGCGTTCGTATTacac                                                                                                                                                               <  1:860942/251‑1 (MQ=255)                                                                              tCGAAAAAATTGCCGATTTCGTCAAAGACAAAAAAATTGAGGGGATCAGCGGGTTGCGCGATGAATCCGACAAAGACGGTATGCGCATCGTCATCGAAATCAAACGTGATGCGGTCGGCGAAGTGGTGTTAAATAACCTGTATGCGTTGACACAAATGCAAGTCACTTTCGGGATTAATATTGTCGCACTTGACCACGGTCAGCCCAAACTATTGAATTTAAAACAGCTCATTGAGGCGTTCGTATTacac                                                                                                                                                               <  1:263060/251‑1 (MQ=255)                                                                              tCGAAAAAATTGCCGATTTCGTCAAAGACAAAAAAATTGAGGGGATCAGCGGGTTGCGCGATGAATCCGACAAAGACGGTATGCGCATCGTCATCGAAATCAAACGTGATGCGGTCGGCGAAGTGGTGTTAAATAACCTGTATGCGTTGACACAAATGCAAGTCACTTTCGGGATTAATATTGTCGCACTTGACCACGGTCAGCCCAAACTATTGAATTTAAAACAGCTCATTGAGGCGTTCGTATTacac                                                                                                                                                               <  1:518213/251‑1 (MQ=255)                                                                                   aaaaTTGCCGATTTCGTCAAAGACAAAAAAATTGAGGGGATCAGCGGGTTGCGCGATGAATCCGACAAAGACGGTATGCGCATCGTCATCGAAATCAAACGTGATGCGGTCGGCGAAGTGGTGTTAAATAACCTGTATGCGTTGACACAAATGCAAGTCACTTTCGGGATTAATATTGTCGCACTTGACCACGGTCAGCCCAAACTATTGAATTTAAAACAGCTCATTGAGGCGTTCGTATTACAccgccg                                                                                                                                                          >  1:718967/1‑251 (MQ=255)                                                                                           cGATTTCGTCAAAGACAAAAAAATTGAGGGGATCAGCGGGTTGCGCGATGAATCCGACAAAGACGGTATGCGCATCGTCATCGAAATCAAACGTGATGCGGTCGGCGAAGTGGTGTTAAATAACCTGTATGCGTTGACACAAATGCAAGTCACTTTCGGGATTAATATTGTCGCACTTGACCACGGTCAGCCCAAACTATTGAATTTAAAACAGCTCATTGAGGCGTTCGTATTACACCGCCGCGAAgtgg                                                                                                                                                  <  1:318713/251‑1 (MQ=255)                                                                                                tCGTCAAAGACAAAAAAATTGAGGGGATCAGCGGGTTGCGCGATGAATCCGACAAAGACGGTATGCGCATCGTCATCGAAATCAAACGTGATGCGGTCGGCGAAGTGGTGTTAAATAACCTGTATGCGTTGACACAAATGCAAGTCACTTTCGGGATTAATATTGTCGCACTTGACCACGGTCAGCCCAAGCTATTGAATTTAAAACAGCTCATTGAGGCGTTCGTATTACACCGCCGCGAAGTGGTGAcc                                                                                                                                             <  1:502206/251‑1 (MQ=255)                                                                                                tCGTCAAAGACAAAAAAATTGAGGGGATCAGCGGGTTGCGCGATGAATCCGACAAAGACGGTATGCGCATCGTCATCGAAATCAAACGTGATGCGGTCGGCGAAGTGGTGTTAAATAACCTGTATGCGTTGACACAAATGCAAGTCACTTTCGGGATTAATATTGTCGCACTTGACCACGGTCAGCCCAAACTATTGAATTTAAAACAGCTCATTGAGGCGTTCGTATTACACCGCCGCGAAGTGGTGAcc                                                                                                                                             <  1:901709/251‑1 (MQ=255)                                                                                                         aCAAAAAAATTGAGGGGATCAGCGGGTTGCGCGATGAATCCGACAAAGACGGTATGCGCATCGTCATCGAAATCAAACGTGATGCGGTCGGCGAAGTGGTGTTAAATAACCTGTATGCGTTGACACAAATGCAAGTCACTTTCGGGATTAATATTGTCGCACTTGACCACGGTCAGCCCAAACTATTGAATTTAAAACAGCTCATTGAGGCGTTCGTATTACACCGCCGCGAAGTGGTGACCCGTCGTAc                                                                                                                                     <  1:334033/250‑1 (MQ=255)                                                                                                          cAAAAAAATTGAGGGGATCAGCGGGTTGCGCGATGAATCCGACAAAGACGGTATGCGCATCGTCATCGAAATCAAACGTGATGCGGTCGGCGAAGTGGTGTTAAATAACCTGTATGCGTTGACACAAATGCAAGTCACTTTCGGGATTAATATTGTCGCACTTGACCACGGTCAGCCCAAACTATTGAATTTAAAACAGCTCATTGAGGCGTTCGTATTACACCGCCGCGAAGTGGTGACCCGTCGTACGa                                                                                                                                   <  1:200110/251‑1 (MQ=255)                                                                                                          cAAAAAAATTGAGGGGATCAGCGGGTTGCGCGATGAATCCGACAAAGACGGTATGCGCATCGTCATCGAAATCAAACGTGATGCGGTCGGCGAAGTGGTGTTAAATAACCTGTATGCGTTGACACAAATGCAAGTCACTTTCGGGATTAATATTGTCGCACTTGACCACGGTCAGCCCAAACTATTGAATTTAAAACAGCTCATTGAGGCGTTCGTATTACACCGCCGCGAAGTGGTGACCCGTCGTACGa                                                                                                                                   <  1:493456/251‑1 (MQ=255)                                                                                                           aaaaaaaTTGAGGGGATCAGCGGGTTGCGCGATGAATCCGACAAAGACGGTATGCGCATCGTCATCGAAATCAAACGTGATGCGGTCGGCGAAGTGGTGTTAAATAACCTGTATGCGTTGACACAAATGCAAGTCACTTTCGGGATTAATATTGTCGCACTTGACCACGGTCAGCCCAAACTATTGAATTTAAAACAGCTCATTGAGGCGTTCGTATTACACCGCCGCGAAGTGGTGACCCGTCGTACGAt                                                                                                                                  <  1:478910/251‑1 (MQ=255)                                                                                                                         gATCAGCGGGTTGCGCGATGAATCCGACAAAGACGGTATGCGCATCGTCATCGAAATCAAACGTGATGCGGTCGGCGAAGTGGTGTTAAATAACCTGTATGCGTTGACACAAATGCAAGTCACTTTCggg                                                                                                                                                                                                                                             >  1:132103/1‑130 (MQ=255)                                                                                                                         gATCAGCGGGTTGCGCGATGAATCCGACAAAGACGGTATGCGCATCGTCATCGAAATCAAACGTGATGCGGTCGGCGAAGTGGTGTTAAATAACCTGTATGCGTTGACACAAATGCAAGTCACTTTCGGGATTAATATTGTCGCACTTGACCACGGTCAGCCCAAACTATTGAATTTAAAACAGCTCATTGAGGCGTTCGTATTACACCGCCGCGAAGTGGTGa                                                                                                                                               <  1:814126/224‑1 (MQ=255)                                                                                                                         gATCAGCGGGTTGCGCGATGAATCCGACAAAGACGGTATGCGCATCGTCATCGAAATCAAACGTGATGCGGTCGGCGAAGTGGTGTTAAATAACCTGTATGCGTTGACACAAATGCAAGTCACTTTCGGGATTAATATTGTCGCACTTGACCACGGTCAGCCCAAACTATTGAATTTAAAACAGCTCATTGAGGCGTTCGTATTACACCGCCGCGAAGTGGTGACCCGTCGTACGa                                                                                                                                   <  1:617920/236‑1 (MQ=255)                                                                                                                         gATCAGCGGGTTGCGCGATGAATCCGACAAAGACGGTATGCGCATCGTCATCGAAATCAAACGTGATGCGGTCGGCGAAGTGGTGTTAAATAACCTGTATGCGTTGACACAAATGCAAGTCACTTTCGGGATTAATATTGTCGCACTTGACCACGGTCAGCCCAAACTATTGAATTTAAAACAGCTCATTGAGGCGTTCGTATTACACCGCCGCGAAGTGGTGACCCGTCGTACGATTTTTGAATTACGCa                                                                                                                    >  1:147982/1‑251 (MQ=255)                                                                                                                         gATCAGCGGGTTGCGCGATGAATCCGACAAAGACGGTATGCGCATCGTCATCGAAATCAAACGTGATGCGGTCGGCGAAGTGGTGTTAAATAACCTGTATGCGTTGACACAAATGCAAGTCACTTTCGGGATTAATATTGTCGCACTTGACCACGGTCAGCCCAAACTATTGAATTTAAAACAGCTCATTGAGGCGTTCGTATTACACCGCCGCGAAGTGGTGACCCGTCGTACGATTTTTGAATTACGCa                                                                                                                    >  1:811395/1‑251 (MQ=255)                                                                                                                           tCAGCGGGTTGCGCGATGAATCCGACAAAGACGGTATGCGCATCGTCATCGAAATCAAACGTGATGCGGTCGGCGAAGTGGTGTTAAATAACCTGTATGCGTTGACACAAATGCAAGTCACTTTCGGGATTAATATTGTCGCACTTGACCACGGTCAGCCCAAACTATTGAATTTAAAACAGCTCATTGAGGCGTTCGTAt                                                                                                                                                                    >  1:891024/1‑201 (MQ=255)                                                                                                                                    tGCGCGATGAATCCGACAAAGACGGTATGCGCATCGTCATCGAAATCAAACGTGATGCGGTCGGCGAAGTGGTGTTAAATAACCTGTATGCGTTGACACAAATGCAAGTCACTTTCGGGATTAATATTGTCGCACTTGACCACGGTCAGCCCAAACTATTGAATTTAAAACAGCTCATTGAGGCGTTCGTATTACACCGCCGCGAAGTGGTGACCCGTCGTACGATTTTTGAATTACGCAAAGCCCGTGaa                                                                                                         <  1:164050/251‑1 (MQ=255)                                                                                                                                        cgATGAATCCGACAAAGACGGTATGCGCATCGTCATCGAAATCAAACGTGATGCGGTCGGCGAAGTGGTGTTAAATAACCTGTATGCGTTGACACAAATGCAAGTCACTTTCGGGATTAATATTGTCGCACTTGACCACGGTCAGCCCAAACTATTGAATTTAAAACAGCTCATTGAGGCGTTCGTATTACACCGCCGCGAAGTGGTGACCCGTCGTACGa                                                                                                                                   >  1:620898/1‑221 (MQ=255)                                                                                                                                              aTCCGACAAAGACGGTATGCGCATCGTCATCGAAATCAAACGTGATGCGGTCGGCGAAGTGGTGTTAAATAACCTGTATGCGTTGACACAAATGCAAGTCACTTTCGGGATTAATATTGTCGCACTTGACCACGGTCAGCCCAAACTATTGAATTTAAAACAGCTCATTGAGGCGTTCGTATTACACCGCCGCGAAGTGGTGACCCGTCGTACGATTTTTGAATTACGCAAAGCCCGTGAACGTACGcaca                                                                                               >  1:835593/1‑251 (MQ=255)                                                                                                                                                  gACAAAGACGGTATGCGCATCGTCATCGAAATCAAACGTGATGCGGTCGGCGAAGTGGTGTTAAATAACCTGTATGCGTTGACACAAATGCAAGTCACTTTCGGGATTAATATTGTCGCACTTGAcc                                                                                                                                                                                                                       <  1:610623/127‑1 (MQ=255)                                                                                                                                                     aaaGACGGTATGCGCATCGTCATCGAAATCAAACGTGATGCGGTCGGCGAAGTGGTGTTAAATAACCTGTATGCGTTGACACAAATGCAAGTCACTTTCGGGATTAATATTGTCGCACTTGACCACGGTCAGCCCAAACTATTGAATTTAAAACAGCTCATTGAGGCGTTCGTATTACACCGCCGCGAAGTGGTGACCCGTCGTACGATTTTTGAATTACGCAAAGCCCGTGAACGTACGCACATCTTAGa                                                                                        <  1:348317/251‑1 (MQ=255)                                                                                                                                                       aGACGGTATGCGCATCGTCATCGAAATCAAACGTGATGCGGTCGGCGAAGTGGTGTTAAATAACCTGTATGCGTTGACACAAATGCAAGTCACTTTCGGGATTAATATTGTCGCACTTGACCACGGTCAGCCCAAACTATTGAATTTAAAACAGCTCATTGAGGCGTTCGTATTACACCGCCGCGAAGTGGTGACCCGTCGTACGATTTTTGAATTACGCAAAGCCCGTGAACGTACGCACATCTTAGAAg                                                                                      >  1:387576/1‑251 (MQ=255)                                                                                                                                                         aCGGTATGCGCATCGTCATCGAAATCAAACGTGATGCGGTCGGCGAAGTGGTGTTAAATAACCTGTATGCGTTGACACAAATGCAAGTCACTTTCGGGATTAATATTGTCGCACTTGACCACGGTCAGCCCAAACTATTGAATTTAAAACAGCTCATTGAGGCGTTCGTATTACACCGCCGCGAAGTGGTGACCCGTCGTACGATTTTTGAATTACGCAAAGCCCGTGAACGTACGCACATCTTAGAAgg                                                                                     <  1:342665/250‑1 (MQ=255)                                                                                                                                                         aCGGTATGCGCATCGTCATCGAAATCAAACGTGATGCGGTCGGCGAAGTGGTGTTAAATAACCTGTATGCGTTGACACAAATGCAAGTCACTTTCGGGATTAATATTGTCGCACTTGACCACGGTCAGCCCAAACTATTGAATTTAAAACAGCTCATTGAGGCGTTCGTATTACACCGCCGCGAAGTGGTGACCCGTCGTACGATTTTTGAATTACGCAAAGCCCGTGAACGTACGCACATCTTAGAAg                                                                                      <  1:524634/249‑1 (MQ=255)                                                                                                                                                         aCGGTATGCGCATCGTCATCGAAATCAAACGTGATGCGGTCGGCGAAGTGGTGTTAAATAACCTGTATGCGTTGACACAAATGCAAGTCACTTTCGGGATTAATATTGTCGCACTTGACCACGGTCAGCCCAAACTATTGAATTTAAAACAGCTCATTGAGGCGTTCGTATTACACCGCCGCGAAGTGGTGACCCGTCGTACGATTTTTGAATTACGCAAAGCCCGTGAACGTACGCACATCTTAGAAg                                                                                      <  1:35010/249‑1 (MQ=255)                                                                                                                                                         aCGGTATGCGCATCGTCATCGAAATCAAACGTGATGCGGTCGGCGAAGTGGTGTTAAATAACCTGTATGCGTTGACACAAATGCAAGTCACTTTCGGGATTAATATTGTCGCACTTGACCACGGTCAGCCCAAACTATTGAATTTAAAACAGCTCATTGAGGCGTTCGTATTACACCGCCGCGAAGTGGTGACCCGTCGTACGATTTTTGAATTACGCAAAGCCCGTGAACGTACGCACATCTTAGAAGGc                                                                                    <  1:112181/251‑1 (MQ=255)                                                                                                                                                          cGGTATGCGCATCGTCATCGAAATCAAACGTGATGCGGTCGGCGAAGTGGTGTTAAATAACCTGTATGCGTTGACACAAATGCAAGTCACTTTCGGGATTAATATTGTCGCACTTGACCACGGTCAGCCCAAACTATTGAATTTAAAACAGCTCATTGAgg                                                                                                                                                                             <  1:776015/161‑1 (MQ=255)                                                                                                                                                          cGGTATGCGCATCGTCATCGAAATCAAACGTGATGCGGTCGGCGAAGTGGTGTTAAATAACCTGTATGCGTTGACACAAATGCAAGTCACTTTCGGGATTAATATTGTCGCACTTGACCACGGTCAGCCCAAACTATTGAATTTAAAACAGCTCATTGAGGCGTTCGTATTACACCGCCGCGAAGTGGTGACCCGTCGTACGATTTTTGAATTACGCAAAGCCCGTGAACGTACGCACATCTTAGAAgg                                                                                     >  1:6852/1‑249 (MQ=255)                                                                                                                                                          cGGTATGCGCATCGTCATCGAAATCAAACGTGATGCGGTCGGCGAAGTGGTGTTAAATAACCTGTATGCGTTGACACAAATGCAAGTCACTTTCGGGATTAATATTGTCGCACTTGACCACGGTCAGCCCAAACTATTGAATTTAAAACAGCTCATTGAGGCGTTCGTATTACACCGCCGCGAAGTGGTGACCCGTCGTACGATTTTTGAATTACGCAAAGCCCGTGAACGTACGCACATCTTAGAAgg                                                                                     >  1:892918/1‑249 (MQ=255)                                                                                                                                                            gTATGCGTATCGTCATCGAAATCAAACGTGATGCGGTCGGCGAAGTGGTGTTAAATAACCTGTATGCGTTGACACAAATGCAAGTCACTTTCGGGATTAATATTGTCGCACTTGACCACGGTCAGCCCAAACTATTGAATTTAAAAc                                                                                                                                                                                         <  1:299004/147‑1 (MQ=255)                                                                                                                                                            gTATGCGCATCGTCATCGAAATCAAACGTGATGCGGTCGGCGAAGTGGTGTTAAATAACCTGTATGCGTTGACACAAATGCAAGTCACTTTCGGGATTAATATTGTCGCACTTGACCACGGTCAGCCCAAACt                                                                                                                                                                                                       <  1:482223/133‑1 (MQ=255)                                                                                                                                                            gTATGCGCATCGTCATCGAAATCAAACGTGATGCGGTCGGCGAAGTGGTGTTAAATAACCTGTATGCGTTGACACAAATGCAAGTCACTTTCGGGATTAATATTGTCGCACTTGACCACGGTCAGCCCAAACTATTGAATTTAAAACAGCTCATTGAGGCGTTCGTATTACACCGCCGCGAAGTGGTGACCCGTCGTACGa                                                                                                                                   <  1:704688/201‑1 (MQ=255)                                                                                                                                                            gTATGCGCATCGTCATCGAAATCAAACGTGATGCGGTCGGCGAAGTGGTGTTAAATAACCTGTATGCGTTGACACAAATGCAAGTCACTTTCGGGATTAATATTGTCGCACTTGACCACGGTCAGCCCAAACTATTGAATTTAAAACAGCTCATTGAGGCGTTCGTATTACACCGCCGCGAAGTGGTGACCCGTCGTACGATTTTTGAATTACGCAAAGCCCGTGAACGTACGCACATCTTAGAAGGCTTg                                                                                 >  1:847640/1‑251 (MQ=255)                                                                                                                                                            gTATGCGCATCGTCATCGAAATCAAACGTGATGCGGTCGGCGAAGTGGTGTTAAATAACCTGTATGCGTTGACACAAATGCAAGTCACTTTCGGGATTAATATTGTCGCACTTGACCACGGTCAGCCCAAACTATTGAATTTAAAACAGCTCATTGAGGCGTTCGTATTACACCGCCGCGAAGTGGTGACCCGTCGTACGATTTTTGAATTACGCAAAGCCCGTGAACGTACGCACATCTTAGAAGGCTTg                                                                                 >  1:824266/1‑251 (MQ=255)                                                                                                                                                            gTATGCGCATCGTCATCGAAATCAAACGTGATGCGGTCGGCGAAGTGGTGTTAAATAACCTGTATGCGTTGACACAAATGCAAGTCACTTTCGGGATTAATATTGTCGCACTTGACCACGGTCAGCCCAAACTATTGAATTTAAAACAGCTCATTGAGGCGTTCGTATTACACCGCCGCGAAGTGGTGACCCGTCGTACGATTTTTGAATTACGCAAAGCCCGTGAACGTACGCACATCTTAGAAGGCTTg                                                                                 >  1:335492/1‑251 (MQ=255)                                                                                                                                                                       gTCATCGAAATCAAACGTGATGCGGTCGGCGAAGTGGTGTTAAATAACCTGTATGCGTTGACACAAATGCAAGTCACTTTCGGGATTAATATTGTCGCACTTGACCACGGTCAGCCCAAACTATTGAATTTAAAACAGCTCATTGAGGCGTTCGTATTACACCGCCGCGAAGTGGTGACCCGTCGTACGATTTTTGAATTACGCAAAGCCCGTGAACGTACGCACATCTTAGAAGGCTTGGCGATTGCCtt                                                                      >  1:17556/1‑251 (MQ=255)                                                                                                                                                                           tCGAAATCAAACGTGATGCGGTCGGCGAAGTGGTGTTAAATAACCTGTATGCGTTGACACAAATGCAAGTCACTTTCGGGATTAATATTGTCGCACTTGACCACGGTCAGCCCAAACTATTGAATTTAAAACAGCTCATTGAGGCGTTCGTATTACACCGCCGCGAAGTGGTGACCCGTCGTACGATTTTTGAATTACGCAAAGCCCGTGAACGTACGCACATCTTAGAAGGCTTGGCGATTGCCTTGGCa                                                                  <  1:256619/251‑1 (MQ=255)                                                                                                                                                                               aaTCAAACGTGATGCGGTCGGCGAAGTGGTGTTAAATAACCTGTATGCGTTGACACAAATGCAAGTCACTTTCGGGATTAATATTGTCGCACTTGACCACGGTCAGCCCAAACTATTGAATTTAAAACAGCTCATTGAGGCGTTCGTATTACAccgccg                                                                                                                                                          <  1:197406/159‑1 (MQ=255)                                                                                                                                                                                aTCAAACGTGATGCGGTCGGCGAAGTGGTGTTAAATAACCTGTATGCGTTGACACAAATGCAAGTCACTTTCGGGATTAATATTGTCGCACTTGACCACGGTCAGCCCAAACTATTGAATTTAAAACAGCTCATTGAGGCGTTCGTATTACACCGCCGCGAAGTGGTGACCCGTCGTACGATTTTTGAATTACGCAAAGCCCGTGAACGTACGCACATCTTAGAAGGCTTGGCGATTGCCTTGGCAAACGt                                                             <  1:820761/251‑1 (MQ=255)                                                                                                                                                                                    aaCGTGATGCGGTCGGCGAAGTGGTGTTAAATAACCTGTATGCGTTGACACAAATGCAAGTCACTTTCGGGATTAATATTGTCGCACTTGACCACGGTCAGCCCAAACTATTGAATTTAAAACAGCTCATTGa                                                                                                                                                                               >  1:663250/1‑133 (MQ=255)                                                                                                                                                                                               gTCGGCGAAGTGGTGTTAAATAACCTGTATGCGTTGACACAAATGCAAGTCACTTTCGGGATTAATATTGTCGCACTTGACCACGGTCAGCCCAAACTATTGAATTTAAAACAGCTCATTGAGGCGTTCGTATTACACCGCCGCGAAGTGGTGACCCGTCGTACGATTTTTGAATTACGCAAAGCCCGTGAACGTACGCACATCTTAGAAGGCTTGGCGATTGCCTTGGCAAACGTCGATCCGATTATCg                                               <  1:50328/250‑1 (MQ=255)                                                                                                                                                                                                          ggtgTTAAATAACCTGTATGCGTTGACACAAATGCAAGTCACTTTCGGGATTAATATTGTCGCACTTGACCACGGTCAGCCCAAACTATTGAATTTAAAACAGCTCATTGa                                                                                                                                                                               >  1:674513/1‑111 (MQ=255)                                                                                                                                                                                                           gtgtTAAATAACCTGTATGCGTTGACACAAATGCAAGTCACTTTCGGGATTAATATTGTCGCACTTGACCACGGTCAGCCCAAACTATTGAATTTAAAACAGCTCATTGAGGCGTTCGTATTACACCGCCGCGAAGTGGTGACCCGTCGTACGATTTTTGAATTACGCAAAGCCCGTGAACGTACGCACATCTTAGAAGGc                                                                                    >  1:850855/1‑201 (MQ=255)                                                                                                                                                                                                             gtTAAATAACCTGTATGCGTTGACACAAATGCAAGTCACTTTCGGGATTAATATTGTCGCACTTGACCACGGTCAGCCCAAACTATTGAATTTAAAACAGCTCATTGAGGCg                                                                                                                                                                           <  1:638276/112‑1 (MQ=255)                                                                                                                                                                                                               tAAATAACCTGTATGCGTTGACACAAATGCAAGTCACTTTCGGGATTAATATTGTCGCACTTGACCACGGTCAGCCCAAACTATTGAATTTAAAACAGCTCAt                                                                                                                                                                                  <  1:314125/103‑1 (MQ=255)                                                                                                                                                                                                               tAAATAACCTGTATGCGTTGACACAAATGCAAGTCACTTTCGGGATTAATATTGTCGCACTTGACCACGGTCAGCCCAAACTATTGAATTTAAAACAGCTCATTGAGGCGTTCGTATTacac                                                                                                                                                               >  1:101946/1‑122 (MQ=255)                                                                                                                                                                                                               tAAATAACCTGTATGCGTTGACACAAATGCAAGTCACTTTCGGGATTAATATTGTCGCACTTGACCACGGTCAGCCCAAACTATTGAATTTAAAACAGCTCATTGAGGCGTTCGTATTACACCGCCGCGAAGTGGTGACCCGTCGTACGATTTTTGAATTACGCAAAGCCCGTGAACGTACGCACATCTTAGa                                                                                        <  1:869833/193‑1 (MQ=255)                                                                                                                                                                                                               tAAATAACCTGTATGCGTTGACACAAATGCAAGTCACTTTCGGGATTAATATTGTCGCACTTGACCACGGTCAGCCCAAACTATTGAATTTAAAACAGCTCATTGAGGCGTTCGTATTACACCGCCGCGAAGTGGTGACCCGTCGTACGATTTTTGAATTACGCAAAGCCCGTGAACGTACGCACATCTTAGAAGGCTTGGCGATTGCCTTGGCAAACGTCGATCCGATTATCGAATTAATCCGCAAAGCa                              >  1:842430/1‑251 (MQ=255)                                                                                                                                                                                                                aaaTAACCTGTATGCGTTGACACAAATGCAAGTCACTTTCGGGATTAATATTGTCGCACTTGACCACGGTCAGCCCAAACTATTGAATTTAAAACAGCTCATTGAGGCGTTCGTATTACACCGCCGCGAAGTGGTGACCCGTCGTACGATTTTTGAATTACGCAAAGCCCGTGAACGTACGCACATCTTAGAAgg                                                                                     <  1:639788/195‑1 (MQ=255)                                                                                                                                                                                                                aaaTAACCTGTATGCGTTGACACAAATGCAAGTCACTTTCGGGATTAATATTGTCGCACTTGACCACGGTCAGCCCAAACTATTGAATTTAAAACAGCTCATTGAGGCGTTCGTATTACACCGCCGCGAAGTGGTGACCCGTCGTACGATTTTTGAATTACGCAAAGCCCGTGAACGTACGCACATCTTAGAAGGCTTGGCGATTGCCTTGGCAAACGTCGATCCGATTATCGAATTAATCCGCAAAGCa                              >  1:809365/1‑250 (MQ=255)                                                                                                                                                                                                                     aCCTGTATGCGTTGACACAAATGCAAGTCACTTTCGGGATTAATATTGTCGCACTTGACCACGGTCAGCCCAAACTATTGAATTTAAAACAGCTCATTGAGGCGTTCGTATTACACCGCCGCGAAGTGGTGACCCGTCGTACGATTTTTGAATTACGCAAAGCCCGTGACCGTACGCACATCTTAGAAGGCTTGGCGATTGCCTTGGCAAACGTCGATCCGATTATCGAATTAATCCGCAAAGc                               >  1:425390/1‑244 (MQ=255)                                                                                                                                                                                                                      ccTGTATGCGTTGACACAAATGCAAGTCACTTTCGGGATTAATATTGTCGCACTTGACCACGGTCAGCCCAAACTATTGAATTTAAAACAGCTCATTGAGGCGTTCGTATTACAcc                                                                                                                                                              <  1:583159/116‑1 (MQ=255)                                                                                                                                                                                                                      ccTGTATGCGTTGACACAAATGCAAGTCACTTTCGGGATTAATATTGTCGCACTTGACCACGGTCAGCCCAAACTATTGAATTTAAAACAGCTCATTGAGGCGTTCGTATTACACCGCCGCGAAGTGGTGACCCGTCGTa                                                                                                                                      >  1:729822/1‑140 (MQ=255)                                                                                                                                                                                                                      ccTGTATGCGTTGACACAAATGCAAGTCACTTTCGGGATTAATATTGTCGCACTTGACCACGGTCAGCCCAAACTATTGAATTTAAAACAGCTCATTGAGGCGTTCGTATTACACCGCCGCGAAGTGGTGACCCGTCGTACGATTTTTGAATTACGCAAAGCCCGTGAACGTACGCACATCTTAGAAGGCTTGGCGATTGCCTTGGCAAACGTCGATCCGATTATCGAATTAATCCGCAAAGc                               <  1:557296/243‑1 (MQ=255)                                                                                                                                                                                                                              cGTTGACACAAATGCAAGTCACTTTCGGGATTAATATTGTCGCACTTGACCACGGTCAGCCCAAACTATTGAATTTAAAACAGCTCATTGAGGCGTTCGTATTACACCGCCGCGAAGTGGTGACCCGTCGTACGATTTTTGAATTACGCAAAGCCCGTGAACGTACGCACATCTTAGAAGGCTTGGCGATTGCCTTGGCAAACGTCGATCCGATTATCGAATTAATCCGCAAAGCACCAAATCCGGaaaa                >  1:83103/1‑250 (MQ=255)                                                                                                                                                                                                                                    cacaAATGCAAGTCACTTTCGGGATTAATATTGTCGCACTTGACCACGGTCAGCCCAAACTATTGAATTTAAAACAGCTCATTGAGGCGTTCGTATTACACCGCCGCGAAGTGGTGACCCGTCGTACGATTTTTGAATTACGCAAAGCCCGTGAACGTACGcac                                                                                                >  1:706393/1‑164 (MQ=255)                                                                                                                                                                                                                                     acaAATGCAAGTCACTTTCGGGATTAATATTGTCGCACTTGACCACGGTCAGCCCAAACTATTGAATTTAAAACAGCTCATTGAGGCGTTCGTATTACACCGCCGCGAAGTGGTGACCCGTCGTACGATTTTTGAATTACGc                                                                                                                     <  1:475562/142‑1 (MQ=255)                                                                                                                                                                                                                                            cAAGTCACTTTCGGGATTAATATTGTCGCACTTGACCACGGTCAGCCCAAACTATTGAATTTAAAACAGCTCATTGAGGCGTTCGTATTACACCGCCGCGAAGTGGTGACCCGTCGTACGATTTTTGAATTACGCAAAGCCCGTGAACGTACGCACATCTTAGAAGGCTTGGCGATTGCCTTGGCAAACGTCGATCCGATTATCGAATTAATCCGCAAAGCACCAAATCCGGAAAACGCCAAACGTGAAt  <  1:683376/250‑1 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                               AATCGAAACCGATGAAAAAGGCCGTGAAAGCATCATCGTGAACGAAATTCCGTATCAAGTGAACAAAGCGAAACTCATCGAAAAAATTGCCGATTTCGTCAAAGACAAAAAAATTGAGGGGATCAGCGGGTTGCGCGATGAATCCGACAAAGACGGTATGCGCATCGTCATCGAAATCAAACGTGATGCGGTCGGCGAAGTGGTGTTAAATAACCTGTATGCGTTGACACAAATGCAAGTCACTTTAGGGATTAATATTGTCGCACTTGACCACGGTCAGCCCAAACTATTGAATTTAAAACAGCTCATTGAGGCGTTCGTATTACACCGCCGCGAAGTGGTGACCCGTCGTACGATTTTTGAATTACGCAAAGCCCGTGAACGTACGCACATCTTAGAAGGCTTGGCGATTGCCTTGGCAAACGTCGATCCGATTATCGAATTAATCCGCAAAGCACCAAATCCGGAAAACGCCAAACGTGAAT  >  1/1751612‑1752096

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑