Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,744,616	C→T	intergenic (+24658/‑50226)	unknown → / → unknown	tRNA‑Asn‑GTT/tRNA‑Leu‑CAA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,744,616	0	C	T	100.0%	199.6 / NA	73	intergenic (+24658/‑50226)	unknown/unknown	tRNA‑Asn‑GTT/tRNA‑Leu‑CAA
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (43/30);  total (43/30)

GTCACCGCGCGATTAAAGCGCGGCGCAATAATGAGGATACAGAACCCTAACGCAATAATGGTATTAATTTCACCAAGCACATCATTGGCTAAA‑CCGCTATCCATCACGCTGCTGAAAATCGAAGTATTCAACAATAAAACCAC‑GGCGACAATAATCCGCTTGGTAATGGATTGGAA‑TTT‑CTCCGCCGTTTCCTGTGAAAAGCCGAAGTGGCGCACTAAAATACCGTTCGGG‑CGCAATACCGCCAGCAGGAAACTAAAGAACCACCAATAGCCCGCCATACTCAGCGACCACTGCCAGAATTCCGCCGGGTTACGGAAGAAGAAAAAGCCGACAAGCTGACAAATTGCCAAAAACCATAACGTTCCTGACAGGGACAAAATTGCTGTGTAAAACAACGCAATCGGCGTATGCCACTGGCTGTCGGAGCGCAACGTATTGATTTCACCGTTCATTAACGCAAAGCGGTGTTTAATTGCCGGTTTGAAT  >  1/1744371‑1744856                                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                                 gTCACCGCGCGATTAAAGCGCGGCGCAATAATGAGGATACAGAACCCTAACGCAATAATGGTATTAATTTCACCAAGCACATCATTGGCTAAA‑CCGCTATCCATCACGCTGCTGAAAATCGAAGTATTCAACAATAAAACCAC‑GGCGACAATAATCCGCTTGGTAATGGATTGGAA‑TTT‑CTCCGCCGTTTCCTGTGAAAAGCCGAAGTGGCGCACTAAAATACCGTTCGGG‑CGCAATACCGCCAGTAGGaa                                                                                                                                                                                                                                             >  1:517411/1‑251 (MQ=255)     ccGCGCGATTAAAGCGCGGCGCAATAATGAGGATACAGAACCCTAACGCAATAATGGTATTAATTTCACCAAGCACATCATTGGCTAAA‑CCGCTATCCATCACGCTGCTGAAAATCGAAGTATTCAACAATAAAACCAC‑GGCGACAATAATCCGCTTGGTAATGGATTGGAA‑TTT‑CTCCGCCGTTTCCTGTGAAAAGCCGAAGTGGCGCACTAAAATACCGTTCGGG‑CGCAATACCGCCAGTAGGAAACTa                                                                                                                                                                                                                                         >  1:182305/1‑251 (MQ=255)            aTTAAAGCGCGGCGCAATAATGAGGATACAGAACCCTAACGCAATAATGGTATTAATTTCACCAAGCACATCATTGGCTAAA‑CCGCTATCCATCACGCTGCTGAAAATCGAAGCATTCAACAATAAAACCAC‑GGCGACAATAATCCGCTTGGTAATGGATTGGAA‑TTT‑CTCCGCCGTTTCCTGTGAAAAGCCGAAGAGGCGCACTAAAATACCGATCGGG‑CGCAATACCGCCAGTAGGAAACTAAAGAac                                                                                                                                                                                                                                   >  1:484257/1‑250 (MQ=255)                   cgcgGCGAAATAATGGGGATACAGACCCCAAACAAAATAATGGTATAAATTTCACCAAAAAAATCTTTGACTAAA‑CCGCTACCCATCACGCTGCAGAAAATCAAATTATTCAACAATAAAACCAC‑GGCGACAATAATCCGCTTGGAAAGGGATTGGAA‑TTT‑CTCCGCCGTTTCCTGTGAAAAGCCGAAGGGGCGCACAAAAATCCCGTTGGGG‑CGCAATACCGCCAGTAGGAAACTAAAGAACCACCAAt                                                                                                                                                                                                                            <  1:195152/250‑1 (MQ=255)                       gcgcAATAATGAGGATACAGAACCCTAACGCAATAATGGTATTAATTTCACCAAGCACATCATTGGCTAAA‑CCGCTATCCATCACGCTGCTGAAAATCGAAGTATTCAACAATAAAACCAC‑GGCGACAATAATCCGCTTGGTAATGGATTGGAA‑TTT‑CTCCGCCGTTTCCTGTGAAAAGCCGAAGTGGCGCACTAAAATACCGTTCGGG‑CGCAATACCGCCAGTAGGAAACTAAAGAACCACCAATAGcc                                                                                                                                                                                                                        >  1:614494/1‑250 (MQ=255)                          cAATAATGAGGATACAGAACCCTAACGCAATAATGGTATTAATTTCACCAAGCACATCATTGGCTAAA‑CCGCTATCCATCACGCTGCTGAAAATCGAAGTATTCAACAATAAAACCAC‑GGCGACAATAATCCGCTTGGTAATGGATTGGAA‑TTT‑CTCCGCCGTTTCCTGTGAAAAGCCGAAGTGGCGCACTAAAATACCGTTCGGG‑CGCAATACCGCCAGTAGGAAACTAAAGAACCACCAAt                                                                                                                                                                                                                            >  1:870273/1‑243 (MQ=255)                            ataatGAGGATACAGAACCCTAACGCAATAATGGTATTAATTTCACCAAGCACATCATTGGCTAAA‑CCGCTATCCATCACGCTGCTGAAAATCGAAGTATTCAACAATAAAACCAC‑GGCGACAATAATCCGCTTGGTAATGGATTGGAA‑TTT‑CTCCGCCGTTTCCTGTGAAAAGCCGAAGTGGCGCACTAAAATACCGTTCGGG‑CGCAATACCGCCAGTAGGAAACTAAAGAacc                                                                                                                                                                                                                                  >  1:741308/1‑235 (MQ=255)                            ataatGAGGATACAGAACCCTAACGCAATAATGGTATTAATTTCACCAAGCACATCATTGGCTAAA‑CCGCTATCCATCACGCTGCTGAAAATCGAAGTATTCAACAATAAAACCAC‑GGCGACAATAATCCGCTTGGTAATGGATTGGAA‑TTT‑CTCCGCCGTTTCCTGTGAAAAGCCGAAGTGGCGCACTAAAATACCGTTCGGG‑CGCAATACCGCCAGTAGGAAACTAAAGAACCACCAATAGCCCGCCAt                                                                                                                                                                                                                  >  1:127345/1‑251 (MQ=255)                              aatGAGGATACAGAACCCTAACGCAATAATGGTATTAATTTCACCAAGCACATCATTGGCTAAA‑CCGCTATCCATCACGCTGCTGAAAATCGAAGTATTCAACAATAAAACCAC‑GGCGACAATAATCCGCTTGGTAATGGATTGGAA‑TTT‑CTCCGCCGTTTCCTGTGAAAAGCCGAAGTGGCGCACTAAAATACCGTTCGGG‑CGCAATACCGCCAGTAGGAAACTAAAGAACCACCAATAGCCCGCCATa                                                                                                                                                                                                                 >  1:182151/1‑250 (MQ=255)                                tggggatacaaaccccaacccaaaaaatGGTATAAATTCCACAAAGCAAATCTTGGGAAAAA‑CCGCTATCCACCCCGCGGCGAAAAATCGAAGTTTTAAACAA‑AAAACCCCGGGCGAAAAAAACCCGC‑TGGGAAAGGGTTGGAATTTT‑CCCCGCCGTTTCCTGTGAAAAGCAGAAGGGGCGCAAAAAAATACCGTCCGGG‑CGAAATACCGCCAGTAGGAAACTAAAGAACCACCAATAGCCCGCCATACTc                                                                                                                                                                                                              <  1:666273/226‑1 (MQ=255)                                  ggaaaaCAGACCCCAAACGAAAAAATGGTATAAATTTCACCAAGCAAATC‑TTGGGAAAACCCCCTACCCACCACGCTGCTAAAAACCAAAGTATCAAACAAAAAACCCAG‑GGGGAAAATAATCCGCTTGGAAATGAATTGAAA‑TTC‑CTCCGCCTTTTCCGGTAAAAAGCCAAAGGGGCGCACTAAAACACCGTCCGGG‑CGCAATACCGCCAGTAGGAAACTAAAGAACCACCAATAGCCCGCCATACTc                                                                                                                                                                                                              <  1:287105/244‑1 (MQ=255)                                  aGGATACAGACCCCAAACGCAAAAAGGTTATAAATTCCACCAACAACATAATTGGCAAAA‑CCGCTATCCACCCCGCTGCTGAAAATCAAATTATTAAAAAAAAAAACCAC‑GGCGAAAAAAATCCCCTGGGTAATGGATGGAAA‑TTT‑CCCCGCCGTTCCCTGTAAAAAGCCGAGGTGGAGCACAAAAATCCCGTCCGGG‑CGCAATACCGCCAGTAGGAAACTAAAGAACCACCAATAGCCCGCCATACTc                                                                                                                                                                                                              <  1:409279/249‑1 (MQ=255)                                     aaaaagaccccaaccgaaaaaatGTTATTATTTCCACAAACCACATAATGGGCAAAC‑CCGCTACCCACCCCGCTGCTAAAAACCGAATTATTCAAAAAAAAAACCCC‑GGCAAAAATAACCCGTTGGGAAAGGGATGGAAA‑TTT‑CCCCGCCGTTTCCTGTGAAAAGCCGAAGGGGCGCACAAAAATACCGTTCGGG‑CGCAATACCGCCAGTAGGAAACTAAAGAACCACCAATAGCCCGCCATACTCAGCGa                                                                                                                                                                                                         <  1:461905/231‑1 (MQ=255)                                                     cAATAATGGTATTAATTTCACCAAGCACATCATTGGCTAAA‑CCGCTATCCATCACGCTGCTGAAAATCGAAGTATTCAACAATAAAACCAC‑GGCGACATTAATCCGCTTGGTAATGGATTGGAA‑TTT‑CTCCGCCGTTTCCTGTGAAAAGCCGAAGTGGCGCACTAAAATACCGTTCGGG‑CGCAATACCGCCAGTAGGAAACTAAAGAACCACCAATAGCCCGCCATACTCAGCGACCACTGCCAGAATTc                                                                                                                                                                                          >  1:644027/1‑250 (MQ=255)                                                     cAATAATGGTATTAATTTCACCAAGCACATCATTGGCTAAA‑CCGCTATCCATCACGCTGCTGAAAATCGAAGTATTCAACAATAAAACCAC‑GGCGACAATAATCCGCTTGGTAATGGATTGGAA‑TTT‑CTCCGCCGTTTCCTGTGAAAAGCCGAAGTGGCGCACTAAAATCCCGTTCGGG‑CGCAATACCGCCAGTAGGAAACTAAAGAACCACCAATAGcc                                                                                                                                                                                                                        >  1:450254/1‑220 (MQ=255)                                                     cAATAATGGTATTAATTTCACCAAGCACATCATTGGCTAAA‑CCGCTATCCATCACGCTGCTGAAAATCGAAGTATTCAACAATAAAACCAC‑GGCGACAATAATCCGCTTGGTAATGGATTGGAA‑TTT‑CTCCGCCGTTTCCTGTGAAAAGCCGAAGTGGCGCACTAAAATACCGTTCGGG‑CGCAATACCGCCAGTAGGAAACTAAAGAACCACCAATAGCCCGCCATACTCAGCGa                                                                                                                                                                                                         >  1:777051/1‑235 (MQ=255)                                                            ggTATTAATTTCACCAAGCACATCATTGGCTAAA‑CCGCTATCCATCACGCTGCTGAAAATCGAAGTATTCAACAATAAAACCAC‑GGCGACAATAATCCGCTTGGTAATGGATTGGAA‑TTT‑CTCCGCCGTTTCCTGTGAAAAGCCGAAGTGGCGCACTAAAATACCGTTCGGG‑CGCAATACCGCCAGTAGGAAACTAAAGAACCACCAATAGCCCGCCATACTCAGCGACCACTGCCAGAATTCCGCCGGGt                                                                                                                                                                                  >  1:504266/1‑251 (MQ=255)                                                                 tAATTTCACCAAGCACATCATTGGCTAAA‑CCGCTATCCATCACGCTGCTGAAAATCGAAGTATTCAACAATAAAACCAC‑GGCGACAATAATCCGCTTGGTAATGGATTGGAA‑TTT‑CTCCGCCGTTTCCTGTGAAAAGCCGAAGTGGCGCACTAAAATACCGTTCGGG‑CGCAATACCGCCAGTAGGAAACTAAAGAACCACCAATAGCCCGCCATACTCAGCGACCa                                                                                                                                                                                                      >  1:418199/1‑226 (MQ=255)                                                                             gCACATCATTGGCTAAA‑CCGCTATCCATCACGCTGCTGAAAATCGAAGTATTCAACAATAAAACCAC‑GGCGACAATAATCCGCTTGGTAATGGATTGGAA‑TTT‑CTCCGCCGTTTCCTGTGAAAAGCCGAAGTGGCGCACTAAAATACCGTTCGGG‑CGCAATACCGCCAGTAGGAAACTAAAGAACCACCAATAGCCCGCCATACTCAGCGACCACTGCCAGAATTCCGCCGGGTTACGGAAGAAGAAAAAg                                                                                                                                                                 >  1:785921/1‑251 (MQ=255)                                                                                catcatTGGCTAAA‑CCGCTATCCATCACGCTGCTGAAAATCGAAGTATTCAACAATAAAACCAC‑GGCGACAATAATCCGCTTGGTAATGGATTGGAA‑TTT‑CTCCGCCGTTTCCTGTGAAAAGCCGAAGTGGCGCACTAAAATACCGTTCGGG‑CGCAATACCGCCAGTAGGAAACTAAAGAACCACCAATAGCCCGCCATACTCAGCGACCACTGCCAGAATTCCGCCGGGTTACGGAAGAAGAAAAAGCCg                                                                                                                                                              >  1:747313/1‑251 (MQ=255)                                                                                  tcatTGGCTAAA‑CCGCTATCCATCACGCTGCTGAAAATCGAAGTATTCAACAATAAAACCAC‑GGCGACAATAATCCGCTTGGTAATGGATTGGAA‑TTT‑CTCCGCCGTTTCCTGTGAAAAGCCGAAGTGGCGCACTAAAATACCGTTCGGG‑CGCAATACCGCCAGTAGGACACTAAAGAACCACCAATAGCCCGCCATACTCAGCGACCACTGCCAGAATTCCGCCGGGTTACGGAAGAAGAAAAAGCCGa                                                                                                                                                             >  1:428202/1‑250 (MQ=255)                                                                                                              cggctaaaaaTCGAAGTATTCAACAAAAAACCCAC‑GGCAAAAATAACCCGCTGGGTAATGGATTGAAT‑TTT‑CTCCGCGGTTCCCTGAGAAAAGCCGAAGGGGCGCACAAAAATGCCGTTCGGG‑CGAAATACCGCCAGTAGGAAACTAAAGAACCACCAATAGCCCGCCATACTCAGCGACCACTGCCAGAATTCCGCCGGGTTACGGAAGAAGAAAAAGCCGACAAGCTGACAAATTGCCAAAAACCATaa                                                                                                                                 <  1:879334/245‑1 (MQ=255)                                                                                                                 caaaaaaTCAAAGTATAAAACAAAAAACCCAC‑GGCGAAAAAAACCCGTTTGGAAAGGGA‑TGGAATTTT‑CCCCGCCGTTTCCTGTAAAAAGCCGAAGTGGCGCACAAAAATCCCGTTCGGG‑CGCAATACCGCCAGTAGGAAACTAAAGAACCACCAATAGCCCGCCATACTCAGCGACCACTGCCAGAATTCCGCCGGGTTACGGAAGAAGAAAAAGCCGACAAGCTGACAAATTGCCAAAAACCATAACg                                                                                                                               <  1:348985/247‑1 (MQ=255)                                                                                                                          gAAGTATTCAACAATAAAACCAC‑GGCGACAATAATCTGCTTGGTAATGGATTGGAA‑TTT‑CTCCGCCGTTTCCTGTGAAAAGCCGAAGTGGCGCACTAAAATACCGTTCGGG‑CGCAATACCGCCAGTAGGAAACTAAAGAACCACCAATAGCCCGCCATACTCAGCGACCACTGCCAGAATTCCGCCGGGTTACGGAAGAAGAAAAAGCCGACAAGCTGACAAATTGCCAAAAACCATAACGTTCCTGACAg                                                                                                                     >  1:576659/1‑251 (MQ=255)                                                                                                                             gTATTCAACAATAAAACCAC‑GGCGACAATAATCCGCTTGGTAATGGATTGGAA‑TTT‑CTCCGCCGTTTCCTGTGAAAAGCCGAAGTGGCGCACTAAAATACCGTTCGGG‑CGCAATACCGCCAGTAGGAAACTAAAGAACCACCAATAGCCCGCCATACTCAGCGACCACTGCCAGAATTCCGCCGGGTTACGGAAGAAGAAAAAGCCGACAAGCTGACAAATTGCCAAAAACCATAACGTTCCTGACAGGGa                                                                                                                  >  1:857771/1‑251 (MQ=255)                                                                                                                               aTTCAACAATAAAACCAC‑GGCGACAATAATCCGCTTGGTAATGGATTGGAA‑TTT‑CTCCGCCGTTTCCTGTGAAAAGCCGAAGTGGCGCACTAAAATACCGTTCGGG‑CGCAATACCGCCAGTAGGAAACTAAAGAACCACCAATAGCCCGCCATACTCAGCGACCACTGCCAGAATTCCGCCGGGTTACGGAAGAAGAAAAAGCCGACAAGCTGACAAATTGCCAAAAACCATAACGTTCCTGACAGGGACa                                                                                                                >  1:312578/1‑251 (MQ=255)                                                                                                                               aTTCAACAATAAAACCAC‑GGCGACAATAATCCGCTTGGAAATGGATTGGAA‑TTT‑CTCCGCCGTTTCCTGTGAAAAGCCGAAGTGGCGCACTAAAATACCGTTCGGG‑CGCAATACCGCCAGTAGGAAACTAAAGAACCACCAATAGCCCGCCATACTAAGCGACCACTGCCAGAATTCCGCCGGGTTACGGAAGAAGAAAAAGCCGACAAGCTGACAAATTGCCAAAAACCCTAACGTTCCTGACAGGGACa                                                                                                                >  1:583547/1‑251 (MQ=255)                                                                                                                                    acaaTAAAACCAC‑GGCGACAATAATCCGCTTGGTAATGGATTGGAA‑TTT‑CTCCGCCGTTTCCTGTGAAAAGCCGAAGTGGCGCACTAAAATACCGTTCGGG‑CGCAATACCGCCAGTAGGAAACTAAAGAACCACCAATAGCCCGCCATACTCAGCGACCACTGCCAGAATTc                                                                                                                                                                                          >  1:48214/1‑172 (MQ=255)                                                                                                                                     caaTAAAACCAC‑GGCGACAATAATCCGCTTGGTAATGGATTGGAA‑TTT‑CTCCGCCGTTTCCTGTGAAAAGCCGAAGTGGCGCACTAAAATACCGTTCGGG‑CGCAATACCGCCAGTAGGAAACTAAAGAACCACCAATAGCCCGCCATACTCAGCGACCACTGCCAGAATTCCGCCGGGTTACGgaag                                                                                                                                                                          >  1:215386/1‑187 (MQ=255)                                                                                                                                     caaTAAAACCAC‑GGCGACAATAATCCGCTTGGTAATGGATTGGAA‑TTT‑CTCCGCCGTTTCCTGTGAAAAGCCGAAGTGGCGCACTAAAATACCGTTCGGG‑CGCAATACCGCCAGTAGGAAACTAAAGAACCACCAATAGCCCGCCATACTCAGCGACCACTGCCAGAATTCCGCCGGGTTACGGAAGAAGAAAAAGCCGACAAGCTGACAAATTGCCAAAAACCATAACGTTCCTGACAGGGACAAAATTg                                                                                                          >  1:870701/1‑251 (MQ=255)                                                                                                                                              ccccggcaaaaataccccGCTTGGTAATGGA‑TGGAA‑TTTCCCCCGCCGTTCCCTGTAAAAAGCCGAGGGGGCGCACAAAAACCCCTTTGGGG‑CGAAATACCGCCAGTAGGAAACTAAAGAACCACCAATAGCCCGCCATACTCAGCGACCACTGCCAGAATTCCGCCGGGTTACGGAAGAAGAAAAAGCCGACAAGCTGACAAATTGCCAAAAACCATAACGTTCCTGACAGGGACAAAATTGCTGTGTaa                                                                                                  <  1:400663/235‑1 (MQ=255)                                                                                                                                                      aCAATAATCCGCTTGGTAATGGATTGGAA‑TTT‑CTCCGCCGTTTCCTGTGAAAAGCCGAAGTGGCGCACTAAAATACCGTTCGGG‑CGCAATACCGCCAGTAGGAAACTAAAGAACCACCAATAGCCCGCCATACTCAGCGACCACTGCCAGAATTCCGCCGGGTTACGGAAGAAGAAAAAGCCGACAAGCTGACAAATTGCCAAAAACCATAACGTTCCTGACAGGGACAAAATTGCTGTGTAAAACAACg                                                                                           >  1:140102/1‑250 (MQ=255)                                                                                                                                                              ccgtttGGT‑ATGGATTTGAA‑TTTCCCCCCCCTTTTCCTGTAAAAAGCCGAAGTGCCGCACAAAAATCCCGTCCGGC‑CCAAATCCCGCCAGTAGGAAACTAAAGAACCACCAATAGCCCGCCATACTCAGCGACCACTGCCAGAATTCCGCCGGGTTACGGAAGAAGAAAAAGCCGACAAGCTGACAAATTGCCAAAAACCATAACGTTCCTGACAGGGACAAAATTGCTGTGTAAAACAACGCAATCGGCg                                                                                  <  1:197765/247‑1 (MQ=255)                                                                                                                                                              ccgttgggaaaTGGATGGGAT‑TTT‑CCCCGCCGTTTCCTGTGAAAAGCCGAAGTGGCGCACAAAAATCCCGTCGGGG‑CGCAATACCGCCAGTAGGAAACTAAAGAACCACCAATAGCCCGCCATACTCAGCGACCACTGCCAGAATTCCGCCGGGTTACGGAAGAAGAAAAAGCCGACAAGCTGACAAATTGCCAAAAACCATAACGTTCCTGACAGGGACAAAATTGCTGTGTAAAACAACGCAATCGGCg                                                                                  <  1:251656/242‑1 (MQ=255)                                                                                                                                                                 cTTGGTAATGGATTGGAA‑TTT‑CTCCGCCGTTTCCTGTGAAAAGCCGAAGTGGCGCACTAAAATACCGTTCGGG‑CGCAATACCGCCAGTAGGAAACTAAAGAACCACCAATAGCCCGCCATACTCAGCGACCACTGCCAGAATTCCGCCGGGTTACGGAAGAAGAAAAAGCCGACGAGCTGACAAATTGCCAAAAACCATAACGTTCCTGACAGGGACAAAATTGCTGTGTAAAACAACGCAATCGGCGTAt                                                                               >  1:43676/1‑251 (MQ=255)                                                                                                                                                                   tGGTAATGGATTGGAA‑TTT‑CTCCGCCGTTTCCTGTGAAAAGCCGAAGTGGCGCACTAAAATACCGTTCGGG‑CGCAATACCGCCAGTAGGAAACTAAAGAACCACCAATAGCCCGCCATACTCAGCGACCACTGCCAGAATTCCGCCGGGTTACGGAAGAAGAAAAAGCCGACAAGCTGACAAATTGCCAAAAACCATAACGTTCCTGACAGGGACAAAATTGCTGTGTAAAACAACGCAATCGGCGTATg                                                                              >  1:587440/1‑250 (MQ=255)                                                                                                                                                                   tGGTAATGGATTGGAA‑TTT‑CTCCGCCGTTTCCTGTGAAAAGCCGAAGTGGCGCACTAAAATACCGTTCGGG‑CGCAATACCGCCAGTAGGAAACTAAAGAACCACCAATAGCCCGCCATACTCAGCGACCACTGCCAGAATTCCGCCGGGGTACGGAAGAAGAAAAAGCCGACAAGCTGACAAATTGCCAAAAACCATAACGTTCCTGACAGGGACAAAATTGCTGTGTAAAACAACGCAATCGGCGTATg                                                                              >  1:412413/1‑250 (MQ=255)                                                                                                                                                                   tGGTAATGGA‑TGGAATTTT‑CCCCGCCGTTCCCTGTAAAAGGCCGAAGTGGCGCACAAAAATCCCGTCCGGG‑CGCAATACCGCCAGTAGGAAACTAAAGAACCACCAATAGCCCGCCATACTCAGCGACCACTGCCAGAATTCCGCCGGGTTACGGAAGAAGAAAAAGCCGACAAGCTGACAAATTGCCAAAAACCATAACGTTCCTGACAGGGACAAAATTGCTGTGTAAaacaa                                                                                             <  1:710858/235‑1 (MQ=255)                                                                                                                                                                     gaagtGGATT‑GAA‑TTTCCTCCGCCGTTTCCTGGGAAAGGCCGAAGGGGCGCACTAAAATACCGTTCGGG‑CGCAATGCCGCCAGTAGGAAACTAAAGAACCACCAATAGCCCGCCATACTCAGCGACCACTGCCAGAATTCCGCCGGGTTACGGAAGAAGAAAAAGCCGACAAGCTGACAAATTGCCAAAAACCATAACGTTCCTGACAGGGACAAAATTGCTGTGTAAAACAACGCAATCGGCGTATGcc                                                                            <  1:478364/246‑1 (MQ=255)                                                                                                                                                                     gTAATGGATTGGAA‑TTT‑CTCCGCCGTTTCCTGTGAAAAGCCGAAGTGGCGCACTAAAATACCGTTCGGG‑CGCAATACCGCCAGTAGGAAACTAAAGAACCACCAATAGCCCGCCATACTCAGCGACCACTGCCAGAATTCCGCCGGGTTACGGAAGAAGaaaaa                                                                                                                                                                  >  1:362188/1‑164 (MQ=255)                                                                                                                                                                     gTAATGGATTGGAA‑TTT‑CTCCGCCGTTTCCTGTGAAAAGCCGAAGTGGCGCACTAAAATACCGTTCGGG‑CGCAATACCGCCAGTAGGAAACTAAAGAACCACCAATAGCCCGCCATACTCAGCGACCACTGCCAGAATTCCGCCGGGTTACGGAAGAAGAAAAAGCCGACAAGCTGACAAATTGCCAAAAACCATAAc                                                                                                                                >  1:304264/1‑198 (MQ=255)                                                                                                                                                                            aTTGGAA‑TTT‑CTCCGCCGTTTCCTGTGAAAAGCCGAAGTGGCGCACTAAAATACCGTTCGGG‑CGCAATACCGCCAGTAGGAAACTAAAGAACCACCAATAGCCCGCCATACTCAGCGACCACTGCCAGAATTCCGCCGGGTTACGGAAGAAGAAAAAGCCGACAAGCTGACAAATTGCCAAAAACCATAACGTTCCTGACAGGGACAAAATTGCTGTGTAAAACAACGCAATCGGCGTATGCCACTgg                                                                       >  1:859597/1‑248 (MQ=255)                                                                                                                                                                               ggAA‑TTT‑CTCCGCCGTTTCCTGTGAAAAGCCGAAGTGGCGCACTAAAATACCGTTCGGG‑CGCAATACCGCCAGTAGGAAACTAAAGAACCACCAATAGCCCGCCATACTCAGCGACCACTGCCAGAATTCCGCCGGGTTACGGAAGAAGAAAAAGCCGACAAGCTGACAAATTGCCAAAAACCATAACGTTCCTGAc                                                                                                                       >  1:515675/1‑197 (MQ=255)                                                                                                                                                                                  aattttcccccccgTTTCCTGTAAAAAGCCAAAGTGGCCCAAAAAAATCCCGTCCGGG‑CGAAATACCGCCAGTAGGAAACTAAAGAACCACCAATAGCCCGCCATACTCAGCGACCACTGCCAGAATTCCGCCGGGTTACGGAAGAAGAAAAAGCCGACAAGCTGACAAATTGCCAAAAACCATAACGTTCCTGACAGGGACAAAATTGCTGTGTAAAACAACGCAATCGGCGTATGCCACTGGCTGTCgg                                                                <  1:660321/240‑1 (MQ=255)                                                                                                                                                                                   tttt‑CCCCGCCTTTTCCTGTGAAAAGCCGAAGTGCCGCACAAAAATCCCGTCCGGG‑CGAAATACCGCCAGTAGGAAACTAAAGAACCACCAATAGCCCGCCATACTCAGCGACCACTGCCAGAATTCCGCCGGGTTACGGAAGAAGAAAAAGCCGACAAGCTGACAAATTGCCAAAAACCATAACGTTCCTGACAGGGACAAAATTGCTGTGTAAAACAACGCAATCGGCGTATGCCACTGGCTGTCGGAg                                                              <  1:781036/250‑1 (MQ=255)                                                                                                                                                                                    tttcccccccctttcccTGTAAAAACCCGAAGTGGCCCACTAAAATCCCGTCCGGG‑CGCAAAACCGCCAGTAGAAAACTAAAGAACCACCAATAGCCCGCCATACTCAGCGACCACTGCCAGAATTCCGCCGGGTTACGGAAGAAGAAAAAGCCGACAAGCTGACAAATTGCCAAAAACCATAACGTTCCTGACAGGGACAAAATTGCTGTGTAAAACAACGCAATCGGCGTATGCCACTGGCTGTCGGAg                                                              <  1:321230/236‑1 (MQ=255)                                                                                                                                                                                                 ttCCTGTGAAAAGCCGAAGTGGCGCACTAAAATACCGTTCGGG‑CGCAATACCGCCAGTAGGAAACTAAAGAACCACCAATAGCCCGCCATACTCAGCGACCACTGCCAGAATTCCGCCGGGTTACGGAAGAAGAAAAAGCCGACAAGCTGACAAATTGCCAAAAACCATAACGTTCCTGACAGGGACAAAATTGCTGTGTAAAACAACGCAATCGGCGTATGCCACTGGCTGTCGGAGCGCAACGTATTGa                                                 >  1:408458/1‑251 (MQ=255)                                                                                                                                                                                                  cccTGTAAAAGGCCGAGGGGGCGCACAAAAATCCCTTGCGGG‑CACAATACCGCCAGTAGGAAACTAAAGAACCACCAATAGCCCGCCATACTCAGCGACCACTGCCAGAATTCCGCCGGGTTACGGAAGAAGAAAAAGCCGACAAGCTGACAAATTGCCAAAAACCATAACGTTCCTGACAGGGACAAAATTGCTGTGTAAAACAACGCAATCGGCGTATGCCACTGGCTGTCGGAGCGCAACGTATTGAt                                                <  1:196001/250‑1 (MQ=255)                                                                                                                                                                                                   ccTGTAAAAAGCCGAGGTGGCGCACAAAAATCCCGTCCGGG‑CGCAATACCGCCAGTAGGAAACTAAAGAACCACCAATAGCCCGCCATACTCAGCGACCACTGCCAGAATTCCGCCGGGTTACGGAAGAAGAAAAAGCCGACAAGCTGACAAATTGCCAAAAACCATAACGTTCCTGACAGGGACAAAATTGCTGTGTAAAACAACGCAATCGGCGTATGCCACTGGCTGTCGGAGCGCAACGTATTGAtt                                               <  1:468361/251‑1 (MQ=255)                                                                                                                                                                                                    gctcggagatgtgtaaaagagacaaaaaaTCCCGTTCGGG‑CGCAATACCGCCAGTAGGAAACTAAAGACCCACCAATAGCCCGCCATACTCAGCGACCACTGCCAGAATTCCGCCGGGTTACGGAAGAAGAAAAAGCCGACAAGCTGACAAATTGCCAAAAACCATAACGTTCCTGACAGGGACAAAATTGCTGTGTAAAACAACGCAATCGGCGTATGCCACTGGCTGTCGGAGCGCAACGTATTGAtt                                               <  1:481914/225‑1 (MQ=255)                                                                                                                                                                                                              ccGAAGTGGCGCACTAAAATACCGTTCGGG‑CGCAATACCGCCAGTAGGAAACTAAAGAACCACCAATAGCCCGCCATACTCAGCGACCACTGCCAGAATTCCGCCGGGTTACGGAAGAAGAAAAAGCCGACAAGCTGACAAATTGCCAAAAACCATAACGTTCCTGACAGGGACAAAATTGCTGTGTAAAACAACGCAATCGGCGTATGCCACTGGCTGTCGGAGCGCAACGTATTGATTTCACCGTTCAt                                    >  1:297605/1‑251 (MQ=255)                                                                                                                                                                                                                gAAGGGGCGCAATAAAATACCGTCGGGG‑CGAAATCCCGCCAGTAGGAAACTAAAGAACCACCAATAGCCCGCCATACTCAGCGACCACTGCCAGAATTCCGCCGGGTTACGGAAGAAGAAAAAGCCGACAAGCTGACAAATTGCCAAAAACCATAACGTTCCTGACAGGGACAAAATTGCTGTGTAAAACAACGCAATCGGCGTATGCCACTGGCTGTCGGAGCGCAACGTATTGATTTCACCGTTCAtt                                   <  1:275524/250‑1 (MQ=255)                                                                                                                                                                                                                 aaggggcccaaaaaaaccccGTTCGGG‑CGAAATCCCGCCAGTAGGAAACTAAAGAACCACCAATAGCCCGCCATACTCAGCGACCACTGCCAGAATTCCGCCGGGTTACGGAAGAAGAAAAAGCCGACAAGCTGACAAATTGCCAAAAACCATAACGTTCCTGACAGGGACAAAATTGCTGTGTAAAACAACGCAATCGGCGTATGCCACTGGCTGTCGGAGCGCAACGTATTGATTTCACCGTTCATTaa                                 <  1:365975/233‑1 (MQ=255)                                                                                                                                                                                                                 aaGTGGCGCACAAAAACCCCGTCCGGG‑CGAAACCCCGCCAGTAGGAAACTAAAGAACCACCAATAGCCCGCCATACTCAGCGACCACTGCCAGAATTCCGCCGGGTTACGGAAGAAGAAAAAGCCGACAAGCTGACAAATTGCCAAAAACCATAACGTTCCTGACAGGGACAAAATTGCTGTGTAAAACAACGCAATCGGCGTATGCCACTGGCTGTCGGAGCGCAACGTATTGATTTCACCGTTCATTaa                                 <  1:74569/251‑1 (MQ=255)                                                                                                                                                                                                                     ggcccacaaaaaTCCCGTCCGGG‑CGAAATACCGCCAGTAGGAAACTAAAGAACCACCAATAGCCCGCCATACTCAGCGACCACTGCCAGAATTCCGCCGGGTTACGGAAGAAGAAAAAGCCGACAAGCTGACAAATTGCCAAAAACCATAACGTTCCTGACAGGGACAAAATTGCTGTGTAAAACAACGCAATCGGCGTATGCCACTGGCTGTCGGAGCGCAACGTATTGATTTCACCGTTCATTAACGc                              <  1:81866/242‑1 (MQ=255)                                                                                                                                                                                                                     ggCGCACAAAAATACCGTTGGGG‑CGAAATACCGCCAGTAGGAAACTAAAGAACCACCAATAGCCCGCCATACTCAGCGACCACTGCCAGAATTCCGCCGGGTTACGGAAGAAGAAAAAGCCGACAAGCTGACAAATTGCCAAAAACCATAACGTTCCTGACAGGGACAAAATTGCTGTGTAAAACAACGCAATCGGCGTATGCCACTGGCTGTCGGAGCGCAACGTATTGATTTCACCGTTCATTAACGc                              <  1:700352/250‑1 (MQ=255)                                                                                                                                                                                                                      cctcattaaaaccccatccGGG‑CGCACTACCGCCAGTAGAAAACTAAAGAACCACCAATAGCCCGACATACTCAGCGACCACTGCCAGAATTCCGCCGGGTTACGGAAGAAGAAAAAGCCGACAAGCTGACAAATTGCCAAAAACCATAACGTTCCTGACAGGGACAAAATTGCTGTGTAAAACAACGCAATCGGCGTATGCCAATGGCTGTCGGAGCGCAACGTATTGATTTCACCGTTCATTAACACaa                            <  1:508085/233‑1 (MQ=255)                                                                                                                                                                                                                         cacaaaaaTCCCGTTGGGG‑CGAAATACCGCCAGTAGGAAACTAAAGAACCACCAATAGCCCGCCATACTCAGCGACCACTGCCAGAATTCCGCCGGGTTACGGAAGAAGAAAAAGCCGACAAGCTGACAAATTGCCAAAAACCATAACGTTCCTGACAGGGACAAAATTGCTGTGTAAAACAACGCAATCGGCGTATGCCACTGGCTGTCGGAGCGCAACGTATTGATTTCACCGTTCATTAACGCAAAGc                         <  1:466689/247‑1 (MQ=255)                                                                                                                                                                                                                         cACTAAAATCCCGTTCGGG‑CGCAATACCGCCAGTAGGAAACTAAAGAACCACCAATAGCCCGCCATACTCAGCGACCACTGCCAGAATTCCGCCGGGTTACGGAAGAAGAAAAAGCCGACAAGCTGACaa                                                                                                                                                  <  1:168683/130‑1 (MQ=255)                                                                                                                                                                                                                         cACTAAAATACCGTTCGGG‑CGCAATACCGCCAGTAGGAAACTAAAGAACCACCAATAGCCCGCCATACTCAGCGACCACTGCCAGAATTCCGCCGGGTTACGGAAGAAGAAAAAGCCGACAAGCTGACAAATTGCCAAAAACCATAACGTTCCTGACAGGGACAAAATTGCTGTGTAAAACAACGCAATCGGCGTATGCCACTGGCTGTCGGAGCGCAACGTATTGATTTCACCGTTCATTAACGc                              >  1:366941/1‑246 (MQ=255)                                                                                                                                                                                                                           caaaaaTCCCGTTCGGG‑CGCAATACCGCCAGTAGGAAACTAAAGAACCACCAATAGCCCGCCATACTCAGCGACCACTGCCAGAATTCCGCCGGGTTACgg                                                                                                                                                                             <  1:477318/99‑1 (MQ=255)                                                                                                                                                                                                                           cTAAAATACCGTTCGGG‑CGCAATACCGCCAGTAGGAAACTAAAGAACCACCAATAGCCCGCCATACTCAGCGACCACTGCCAGAATTCCGCCGGGTTACGGAAGAAGAAAAAGCCGACAAGCTGACAAATTGCCAAAAACCATAACGTTCCTGACAGGGACAAAAt                                                                                                            >  1:579888/1‑166 (MQ=255)                                                                                                                                                                                                                           cTAAAATACCGTTCGGG‑CGCAATACCGCCAGTAGGAAACTAAAGAACCACCAATAGCCCGCCATACTCAGCGACCACTGCCAGAATTCCGCCGGGTTACGGAAGAAGAAAAAGCCGACAAGCTGACAAATTGCCAAAAACCATAACGTTCCTGACAGGGACAAAATTGCTGTGTAAAACAACGCAATCGGCGTATGCCACTGGCTGTCGGAGCGCAAc                                                        >  1:653589/1‑218 (MQ=255)                                                                                                                                                                                                                           cTAAAATACCGTTCGGG‑CGCAATACCGCCAGTAGGAAACTAAAGAACCACCAATAGCCCGCCATACTCAGCGACCACTGCCAGAATTCCGCCGGGTTACGGAAGAAGAAAAAGCCGACAAGCTGACAAATTGCCAAAAACCATAACGTTCCTGACAGGGACAAAATTGCTGTGTAAAACAACGCAATCGGCGTATGCCACTGGCTGTCGGAGCGCAACGTATTGATTTCAc                                           >  1:206376/1‑231 (MQ=255)                                                                                                                                                                                                                           cTAAAATACCGTTCGGG‑CGCAATACCGCCAGTAGGAAACTAAAGAACCACCAATAGCCCGCCATACTCAGCGACCACTGCCAGAATTCCGCCGGGTTACGGAAGAAGAAAAAGCCGACAAGCTGACAAATTGCCAAAAACCATAACGTTCCTGACAGGGACAAAATTGCTGTGTAAAACAACGCAATCGGCGTATGCCACTGGCTGTCGGAGCGCAACGTATTGATTTCACCGTTCATTAACGCAAAGCgg                       >  1:807924/1‑251 (MQ=255)                                                                                                                                                                                                                           cTAAAATACCGTTCGGG‑CGCAATACCGCCAGTAGGAAACTAAAGAACCACCAATAGCCCGCCATACTCAGCGACCACTGCCAGAATTCCGCCGGGTTACGGAAGAAGAAAAAGCCGACAAGCTGACAAATTGCCAAAAACCATAACGTTCCTGACAGGGACAAAATTGCTGTGTAAAACAACGCAATCGGCGTATGCCACTGGCTGTCGGAGCGCAACGTATTGATTTCACCGTTCATTAACGCAAAGCgg                       >  1:379722/1‑251 (MQ=255)                                                                                                                                                                                                                           atAAAATCCCG‑TCGGGCCGCAAAACCGCCAGTAGAAAACTAAAGACCCACCAATAGCCCGCCATACTCAGCGACCACTGCCAGAATTCCGCCGGGTTACGGAAGAAGAAAAAGCCGGCAAGCTGACAAATTGCCAAAAACCATAACGTTCCTGACAGGGACAAAATTGCTGTGTAAAACAACGCAATCGGCGTATGCCACTGGCTGTCGGAGCGCAACGTATTGATTTCACCGTTCATTAACGCAAAGCgg                       <  1:605033/250‑1 (MQ=255)                                                                                                                                                                                                                             aaaaTACCGTTCGGG‑CGCAATACCGCCAGTAGGAAACTAAAGAACCACCAATAGCCCGCCATACTCAGCGACCACTGCCAGAAt                                                                                                                                                                                            >  1:737875/1‑84 (MQ=255)                                                                                                                                                                                                                             aaaaTACCGTTCGGG‑CGCAATACCGCCAGTAGGAAACTAAAGAACCACCAATAGCCCGCCATACTCAGCGACCACTGCCAGAATTCCGCCGGGTTACGGAAGAAGAAAAAGCCGACAAGCTGACAAATTGCCAAAAACCATAACGTTCCTGACAGGGACAAAATTGCTGTGTAAAACAACGCAATCGGCGTATGCCACTGGCTGTCGGAGCGCAACGTATTGATTTCACCGTTCATTAACGCAAAGCGgtg                     >  1:751661/1‑251 (MQ=255)                                                                                                                                                                                                                                atcccGTCCGGG‑CGCAATCCCGCCAGTAGGAAACTAAAGAACCACCAATAGCCCGCCATACTCAGCGACCACTGCCAGAATTCCGCCGGGTTACGGAAGAAGAAAAAGCCGACAAGCTGACAAATTGCCAAAAACCATAACGTTCCTGACAGGGACAAAATTGCTGTGTAAAACAACGCAATCGGCGTATGCCACTGGCTGTCGGAGCGCAACGTATTGATTTCACCGTTCATTAACGCAAAGCGGTGttt                  <  1:50272/248‑1 (MQ=255)                                                                                                                                                                                                                                   ccGTTGGGG‑CGCAATACCGCCAGTAGGAAACTAAAGAACCACCAATAGCCCGCCATACTCAGCGACCACTGCCAGAATTCCGCCGGGTTACGGAAGAAGAAAAAGCCGACAAGCTGACAAATTGCCAAAAACCATAACGTTCCTGACAGGGACAAAATTGCTGTGTAAAACAACGCAATCGGCGTATGCCACTGGCTGTCGGAGCGCAACGTATTGATTTCACCGTTCATTAACGCAAAGCGGTGTTTAAt               <  1:665015/251‑1 (MQ=255)                                                                                                                                                                                                                                          gg‑CGCAATACCGCCAGTAGGAAACTAAAGAACCACCAATAGCCCGCCATACTCAGCGACCACTGCCAGAATTCCGCCGGGTTACGGAAGAAGAAAAAGCCGACAAGCTGACAAATTGCCAAAAACCATAACGTTCCTGACAGGGACAAAAt                                                                                                            >  1:289152/1‑151 (MQ=255)                                                                                                                                                                                                                                                 aTACCGCCAGTAGGAAACTAAAGAACCACCAATAGCCCGCCATACTCAGCGACCACTGCCAGAATTCCGCCGGGTTACGGAAGAAGAAAAAGCCGACAAGCTGACAAATTGCCAAAAACCATAACGTTCCTGACAGGGACAAAATTGCTGTGTAAAACAACGCAATCGGCGTATGCCACTGGCTGTCGGAGCGCAACGTATTGATTTCACCGTTCATTAACGCAAAGCGGTGTTTAATTGCCGGTTTGAAt  >  1:119954/1‑251 (MQ=255)                                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                                 GTCACCGCGCGATTAAAGCGCGGCGCAATAATGAGGATACAGAACCCTAACGCAATAATGGTATTAATTTCACCAAGCACATCATTGGCTAAA‑CCGCTATCCATCACGCTGCTGAAAATCGAAGTATTCAACAATAAAACCAC‑GGCGACAATAATCCGCTTGGTAATGGATTGGAA‑TTT‑CTCCGCCGTTTCCTGTGAAAAGCCGAAGTGGCGCACTAAAATACCGTTCGGG‑CGCAATACCGCCAGCAGGAAACTAAAGAACCACCAATAGCCCGCCATACTCAGCGACCACTGCCAGAATTCCGCCGGGTTACGGAAGAAGAAAAAGCCGACAAGCTGACAAATTGCCAAAAACCATAACGTTCCTGACAGGGACAAAATTGCTGTGTAAAACAACGCAATCGGCGTATGCCACTGGCTGTCGGAGCGCAACGTATTGATTTCACCGTTCATTAACGCAAAGCGGTGTTTAATTGCCGGTTTGAAT  >  1/1744371‑1744856

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑