Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,741,803	T→C	intergenic (+21845/‑53039)	unknown → / → unknown	tRNA‑Asn‑GTT/tRNA‑Leu‑CAA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,741,803	0	T	C	100.0%	216.7 / NA	77	intergenic (+21845/‑53039)	unknown/unknown	tRNA‑Asn‑GTT/tRNA‑Leu‑CAA
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (42/35);  total (42/35)

GTCAGAAAACGTCCGCCGCCCGGCATAGCAATATCGCCCACCAACATGGTCGGCAAACCCGCCGCCTTGGCGCGTTGCCCCAAACGTTGCAAGTAGCTAATCATGTCAGGGTGTCCGTAATAGCGGTTTTTATTCA‑TGCGAATCACTTGATAGCCGTCGCCTTTATAAGGCAAAGGCTGGGCGCCTATGATACAACCGTTGGAATAACTGCCGATAGCCTGCGATTTCCCGTCTTCTGCGGGAATCGGACGTTTAATTTGTTGCCAATCTTGCGGCGCGGCAAAAGCGTTGGCGGCGTAAAAAACGCTGCTGAAAACGACCGCACTTTTGATGAATTGGAGAAAGGTTTTATTCATGATGGCATCCGTTTTTCAGGTTTATCGGCATTGCGCTTTAAAACGCAACAAATGATCCAACAATACAATCGCCGTCATAGCTTCCGCAATCGGCACGGCGCGGATGCCGACACAAGGAT  >  1/1741572‑1742046                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                    gTCAGAAAACGTCCGCCGCCCGGCATAGCAATATCGCCCACCAACATGGTCGGCAAACCCGCCGCCTTGGCGCGTTGCCCCAAACGTTGCAAGTAGCTAATCATGTCAGGGTGTCCGTAATAGCGGTTTTTATTCA‑TGCGAATCACTTGATAGCCGTCGCCTTTATAAGGCAAAAGCTGGGCGCCTATGATACAACCGTTGGAATAACTGCCGATAGCCTGCGATTTCCCGCCTTCTGCGGGAATCGGACg                                                                                                                                                                                                                                  >  1:571433/1‑251 (MQ=255)                       gCATAGCAATATCGCCCACCAACATGGTCGGCAAACCCGCCGCCTTGGCGCGTTGCCCCAAACGTTGCAAGTAGCTAATCATGTCAGGGTGTCCGTAATAGCGGTTTTTATTCA‑TGCGAATCACTTGATAGCCGTCGCCTTTATAAGGCAAAGGCTGGGCGCCTATGATACAACCGTTGGAATAACTGCCGATAGCCTGCGATTTCCCGCCTTCTGCGGGAATCGGACGTTTaa                                                                                                                                                                                                                             <  1:882240/234‑1 (MQ=255)                       gCATAGCAATATCGCCCACCAACATGGTCGGCAAACCCGCCGCCTTGGCGCGTTGCCCCAAACGTTGCAAGTAGCTAATCATGTCAGGGTGTCCGTAATAGCGGTTTTTATTCA‑TGCGAATCACTTGATAGCCGTCGCCTTTATAAGGCAAAGGCTGGGCGCCTATGATACAACCGTTGGAATAACTGCCGATAGCCTGCGATTTCCCGCCTTCTGCGGGAATCGGACGTTTAATTTGTTGCCAATCTTg                                                                                                                                                                                                             >  1:728986/1‑250 (MQ=255)                       gCATAGCAATATCGCCCACCAACATGGTCGGCAAACCCGCCGCCTTGGCGCGTTGCCCCAAACGTTGCAAGTAGCTAATCATGTCAGGGTGTCCGTAATAGCGGTTTTTATTCA‑TGCGAATCACTTGATAGCCGTCGCCTTTATAAGGCAAAGGCTGGGCGCCTATGATACAACCGTTGGAATAACTGCCGATAGCCTGCGATTTCCCGCCTTCTGCGGGAATCGGACGTTTAATTTGTTGCCAATCTTg                                                                                                                                                                                                             >  1:182247/1‑250 (MQ=255)                       gCATAGCAATATCGCCCACCAACATGGTCGGCAAACCCGCCGCCTTGGCGCGTTGCCCCAAACGTTGCAAGTAGCTAATCATGTCAGGGTGTCCGTAATAGCGGTTTTTATTCA‑TGCGAATCACTTGATAGCCGTCGCCTTTATAAGGCAAAGGCTGGGCGCCTATGATACAACCGTTGGAATAACTGCCGATAGCCTGCGATTTCCCGCCTTCTGCGGGAATCGGACGTTTAATTTGTTGCCAATCTTg                                                                                                                                                                                                             >  1:260197/1‑250 (MQ=255)                         aTAGCAATATCGCCCACCAACATGGTCGGCAAACCCGCCGCCTTGGCGCGTTGCCCCAAACGTTGCAAGTAGCTAATCATGTCAGGGTGTCCGTAATAGCGGTTTTTATTCA‑TGCGAATCACTTGATAGCCGTCGCCTTTATAAGGCAAAGGCTGGGCGCCTATGATACAACCGTTGGAATAACTGCCGATAGCCTGCGATTTCCCGCCTTCTGCGGGAATCGGACGTTTAATTTGTTGCCAATCTTgcgg                                                                                                                                                                                                          <  1:113461/251‑1 (MQ=255)                            gCAATATCGCCCACCAACATGGTCGGCAAACCCGCCGCCTTGGCGCGTTGCCCCAAACGTTGCAAGTAGCTAATCATGTCAGGGTGTCCGTAATAGCGGTTTTTATTCA‑TGCGAATCACTTGATAGCCGTCGCCTTTATAAGGCAAAGGCTGGGCGCCTATGATACAACCGTTGGAATAACTGCCGATAGCCTGCGATTTCCCGCCTTCTGCGGGAATCGGACGTTTAATTTGTTGCCAATCTTgcggcg                                                                                                                                                                                                        >  1:858620/1‑250 (MQ=255)                                                                             gCCCCAAACGTTGCAAGTAGCTAATCATGTCAGGGTGTCCGTAATAGCGGTTTTTATTCA‑TGCGAATCACTTGATAGCCGTCGCCTTTATAAGGCAAAGGCTGGGCGCCTATGATACAACCGTTGGAATAACTGCCGATAGCCTGCGATTTCCCGCCTTCTGCGGGAATCGGACGTTTAATTTGTTGCCAATCTTGCGGCGCGGCAAAAGCGTTGGCGGCGTAAAAAACGCTGCTGAAAACGACCGCACtt                                                                                                                                                      >  1:486449/1‑251 (MQ=255)                                                                               cccAAACGTTGCAAGTAGCTAATCATGTCAGGGTGTCCGTAATAGCGGTTTTTATTCA‑TGCGAATCACTTGATAGCCGTCGCCTTTATAAGGCAAAGGCTGGGCGCCTATGATACAACCGTTGGAATAACTGCCGATAGCCTGCGATTTCCCGCCTTCTGCGGGAATCGGACGTTTAATTTGTTGCCAAt                                                                                                                                                                                                                 >  1:817410/1‑190 (MQ=255)                                                                                  aaaCGTTGCAAGTAGCTAATCATGTCAGGGTGTCCGTAATAGCGGTTTTTATTCA‑TGCGAATCACTTGATAGCCGTCGCCTTTATAAGGCAAAGGCTGGGCGCCTATGATACAACCGTTGGAATAACTGCCGATAGCCTGCGATTTCCCGCCTTCTGCGGGAATCGGACGTTTAATTTGTTGCCAATCTTGCGGCGCGGCAAAAGCGTTGGCGGCGTAAAAAACGCTGCTGAAAACGACCGCACTTTtgat                                                                                                                                                 >  1:665436/1‑251 (MQ=255)                                                                                   aaCGTTGCAAGTAGCTAATCATGTCAGGGTGTCCGTAATAGCGGTTTTTATTCA‑TGCGAATCACTTGATAGCCGTCGCCTTTATAAGGCAAAGGCTGGGCGCCTATGATACAACCGTTGGAATAACTGCCGATAGCCTGCGATTTCCCGCCTTCTGCGGGAATCGGACGTTTAATTTGTTGCCAATCTTGCGGCGCGGCAAAAGCGTTGGCGGCGTAAAAAACGCTGCTGAAAACGACCGCACTTTtgatg                                                                                                                                                <  1:79430/251‑1 (MQ=255)                                                                                    aCGTTGCAAGTAGCTAATCATGTCAGGGTGTCCGTAATAGCGGTTTTTATTCA‑TGCGAATCACTTGATAGCCGTCGCCTTTATAAGGCAAAGGCTGGGCGCCTATGATACAACCGTTGGAATAACTGCCGATAGCCTGCGATTTCCCGCCTTCTGCGGGAATCGGACGTTTAATTTGTTGCCAATCTTGCGGCGCGGCAAAAGCGTTGGCGGCGTAAAAAACGCTGCTGAAAACGACCGCACTTTtgatga                                                                                                                                               >  1:779753/1‑251 (MQ=255)                                                                                              tAGCTAATCATGTCAGGGTGTCCGTAATAGCGGTTTTTATTCA‑TGCGAATCACTTGATAGCCGTCGCCTTTATAAGGCAAAGGCTGGGCGCCTATGATACAACCGTTGGAATAACTGCCGATAGCCTGCGATTTCCCGCCTTCTGCGGGAATCGGACGTTTAATTTGTTGCCAATCTTGCGGCGCGGCAAAAGCGTTGGCGGCGTAAAAAACGCTGCTGAAAACGACCGCACTTTTGATGAATTGGAGaaa                                                                                                                                     >  1:41664/1‑251 (MQ=255)                                                                                              tAGCTAATCATGTCAGGGTGTCCGTAATAGCGGTTTTTATTCA‑TGCGAATCACTTGATAGCCGTCGCCTTTATAAGGCAAAGGCTGGGCGCCTATGATACAACCGTTGGAATAACTGCCGATAGCCTGCGATTTCCCGCCTTCTGCGGGAATCGGACGTTTAATTTGTTGCCAATCTTGCGGCGCGGCAAAAGCGTTGGCGGCGTAAAAAACGCTGCTGAAAACGACCGCACTTTTGATGAATTGGAGaaa                                                                                                                                     >  1:401418/1‑251 (MQ=255)                                                                                                    aTCATGTCAGGGTGTCCGTAATAGCGGTTTTTATTCA‑TGCGAATCACTTGATAGCCGTCGCCTTTATAAGGCAAAGGCTGGGCGCCTATGATACAACCGTTGGAATAACTGCCGATAGCCTGCGATTTCCCGCCTTCTGCGGGAATCGGACGTTTAATTTGTTGCCAATCTTGCGGCGCGGCAAAAGCGTTGGCGGCGTAAAAAACGCTGCTGAAAACGACCGCACTTTTGATGAATTGGAGAAAGGtttt                                                                                                                               >  1:475030/1‑251 (MQ=255)                                                                                                       aTGTCAGGGTGTCCGTAATAGCGGTTTTTATTCA‑TGCGAATCACTTGATAGCCGTCGCCTTTATAAGGCAAAGGCTGGGCGCCTATGATACAACCGTTGGAATAACTGCCGATAGCCTGCGATTTCCCGCCTTCTGCGGGAATCGGACGTTTAATTTGTTGCCAATCTTGCGGCGCGGCAAAAGCGTTGGCGGCGTAAAAAACGCTGCTGAAAACGACCGCACTTTTGATGAAt                                                                                                                                             >  1:143953/1‑234 (MQ=255)                                                                                                       aTGTCAGGGTGTCCGTAATAGCGGTTTTTATTCA‑TGCGAATCACTTGATAGCCGTCGCCTTTATAAGGCAAAGGCTGGGCGCCTATGATACAACCGTTGGAATAACTGCCGATAGCCTGCGATTTCCCGCCTTCTGCGGGAATCGGACGTTTAATTTGTTGCCAATCTTGCGGCGCGGCAAAAGCGTTGGCGGCGTAAAAAACGCTGCTGAAAACGACCGCACTTTTGATGAATTGGAGAAAGGTTTTAtt                                                                                                                            >  1:278444/1‑251 (MQ=255)                                                                                                       aTGTCAGGGTGTCCGTAATAGCGGTTTTTATTCA‑TGCGAATCACTTGATAGCCGTCGCCTTTATAAGGCAAAGGCTGGGCGCCTATGATACAACCGTTGGAATAACTGCCGATAGCCTGCGATTTCCCGCCTTCTGCGGGAATCGGACGTTTAATTTGTTGCCAATCTTGCGGCGCGGCAAAAGCGTTGGCGGCGTAAAAAACGCTGCTGAAAACGACCGCACTTTTGATGAATTGGAGAAAGGTTTTAtt                                                                                                                            >  1:473634/1‑251 (MQ=255)                                                                                                        tgttaGGGTGTTCGTAATAGGGGTTTTTAATCAGTG‑GAATCACTTGATAGCCGGCGCCTTTATAAGGCAAAGGCTTGGCGGCTTTGATACAACCGTTGGAAAAACTGGCGATTGGCTGGGATTTCCCGCCTTTTGGGGGAATCGGACCTTTAATTTTTTGCCAATCTTGCGGGGCGGCAAAAGCGTTGGGGGCGTTAAAAACGCTGCTGAAAACGACCGCACTTTTGATGAATTGGAGAAAGGTTTTATTc                                                                                                                           <  1:239903/247‑1 (MQ=255)                                                                                                        tGTCAGGGTGTCCGTAATAGCGGTTTTTATTCA‑TGCGAATCACTTGATAGCCGTCGCCTTTATAAGGCAAAGGCTGGGCGCCTATGATACAACCGTTGGAATAACTGCCGATAGCCTGCGATTTCCCGCCTTCTGCGGGAATCGGACGTTTAATTTGTTGCCAATCTTgcgg                                                                                                                                                                                                          <  1:897005/172‑1 (MQ=255)                                                                                                        tGTCAGGGTGTCCGTAATAGCGGTTTTTATTCA‑TGCGAATCACTTGATAGCCGTCGCCTTTATAAGGCAAAGGCTGGGCGCCTATGATACAACCGTTGGAATAACTGCCGATAGCCTGCGATTTCCCGCCTTCTGCGGGAATCGGACGTTTAATTTGTTGCCAATCTTGCGGCGCGGCAAAAGCGTTGGCGGCGTAAAAAACGCTGCTGAAAACGACCGCACTTTTGATGAATTGGAGAAAGGTTTTAtt                                                                                                                            >  1:815745/1‑250 (MQ=255)                                                                                                        tGTCAGGGTGTCCGTAATAGCGGTTTTTATTCA‑TGCGAATCACTTGATAGCCGTCGCCTTTATAAGGCAAAGGCTGGGCGCCTATGATACAACCGTTGGAATAACTGCCGATAGCCTGCGATTTCCCGCCTTCTGCGGGAATCGGACGTTTAATTTGTTGCCAATCTTGCGGCGCGGCAAAAGCGTTGGCGGCGTAAAAAACGCTGCTGAAAACGACCGCACTTTTGATGAATTGGAGAAAGGTTTTATTc                                                                                                                           <  1:741824/251‑1 (MQ=255)                                                                                                              ggTGTCCGTAATAGCGGTTTTTATTCA‑TGCGAATCACTTGATAGCCGTCGCCTTTATAAGGCAAAGGCTGGGCGCCTATGATACAACCGTTGGAATAACTGCCGATAGCCTGCGATTTCCCGCCTTCTGCGGGAATCGGACGTTTAATTTGTTGCCAATCTTGCGGCGCGGCAAAAGCGTTGGCGGCGTAAAAAACGCTGCTGAAAACGACCGCACTTTTGATGAATTGGAGAAAGGTTTTATTCatgat                                                                                                                      <  1:202378/250‑1 (MQ=255)                                                                                                              ggTGTCCGTAATAGCGGTTTTTATTCA‑TGCGAATCACTTGATAGCCGTCGCCTTTATAAGGCAAAGGCTGGGCGCCTATGATACAACCGTTGGAATAACTGCCGATAGCCTGCGATTTCCCGCCTTCTGCGGGAATCGGACGTTTAATTTGTTGCCAATCTTGCGGCGCGGCAAAAGCGTTGGCGGCGTAAAAAACGCTGCTGAAAACGACCGCACTTTTGATGAATTGGAGAAAGGTTTTATTCatg                                                                                                                        <  1:13474/248‑1 (MQ=255)                                                                                                                 gtCCGTAATAGCGGTTTTTATTCA‑TGCGAATCACTTGATAGCCGTCGCCTTTATAAGGCAAAGGCTGGGCGCCTATGATACAACCGTTGGAATAACTGCCGATAGCCTGCGATTTCCCGCCTTCTGCGGGAATCGGACGTTTAATTTGTTGCCAATCTTGCGGCGCGGCAAAAGCg                                                                                                                                                                                             >  1:97055/1‑176 (MQ=255)                                                                                                                   ccGTAATAGCGGTTTTTATTCA‑TGCGAATCACTTGATAGCCGTCGCCTTTATAAGGCAAAGGCTGGGCGCCTATGATACAACCGTTGGAATAACTGCCGATAGCCTGCGATTTCCCGCCTTCTGCGGGAATCGGACGTTTAATTTGTTGCCAATCTTGCGGCGCGGCAAAAGCGTTGGCGGCGTAAAAAACGCTGCTGAAAACGACCGCACTTTTGATGAATTGGAGAAAGGTTTTATTCatgat                                                                                                                      <  1:695488/245‑1 (MQ=255)                                                                                                                   ccGTAATAGCGGTTTTTATTCA‑TGCGAATCACTTGATAGCCGTCGCCTTTATAAGGCAAAGGCTGGGCGCCTATGATACAACCGTTGGAATAACTGCCGATAGCCTGCGATTTCCCGCCTTCTGCGGGAATCGGACGTTTAATTTGTTGCCAATCTTGCGGCGCGGCAAAAGCGTTGGCGGCGTAAAAAACGCTGCTGAAAACGACCGCACTTTTGATGAATTGGAGAAAGGTTTTATTCATGATGGCAt                                                                                                                 >  1:409720/1‑250 (MQ=255)                                                                                                                              gTTTTTATTCA‑TGCGAATCACTTGATAGCCGTCGCCTTTATAAGGCAAAGGCTGGGCGCCTATGATACAACCGTTGGAATAACTGCCGATAGCCTGCGATTTCCCGCCTTCTGCGGGAATCGGACGTTTAATTTGTTGCCAATCTTGCGGCGCGGCAAAAGCGTTGGCGGCGTAAAAAACGCTGCTGAAAACGACCGCACTTTTGATGAATTGGAGAAAGGTTTTATTCATGATGGCATCCGTTTTTCAgg                                                                                                     >  1:776766/1‑251 (MQ=255)                                                                                                                                       cA‑TGCGAATCACTTGATAGCCGTCGCCTTTATAAGGCAAAGGCTGGGCGCCTATGATACAACCGTTGGAATAACTGCCGATAGCCTGCGATTTCCCGCCTTCTGCGGGAATCGGACGTTTAATTTGTTGCCAATCTTGCGGCGCGGCAAAAGCGTTGGCGGCGTAAAAAACGCTGCTGAAAACGACCGCACTTTTGATGAATTGGAGAAAGGTTTTATTCATGATGGCATCCGTTTTTCAGGTTTATCgg                                                                                             >  1:370752/1‑250 (MQ=255)                                                                                                                                                   cTTGATAGCCGTCGCCTTTATAAGGCAAAGGCTGGGCGCCTATGATACAACCGTTGGAATAACTGCCGATAGCCTGCGATTTCCCGCCTTCTGCGGGAATCGGACGTTTAATTTGTTGCCAATCTTGCGGCGCGGCAAAAGCGTTGGCGGCGTAAAAAACGCTGCTGAAAACGACCGCACTTTtgatg                                                                                                                                                <  1:655993/188‑1 (MQ=255)                                                                                                                                                   cTTGATAGCCGTCGCCTTTATAAGGCAAAGGCTGGGCGCCTATGATACAACCGTTGGAATAACTGCCGATAGCCTGCGATTTCCCGCCTTCTGCGGGAATCGGACGTTTAATTTGTTGCCAATCTTGCGGCGCGGCAAAAGCGTTGGCGGCGTAAAAAACGCTGCTGAAAACGACCGCACTTTTGATGAATTGGAGAAAGGTTTTATTCatgat                                                                                                                      >  1:352417/1‑214 (MQ=255)                                                                                                                                                   cTTGATAGCCGTCGCCTTTATAAGGCAAAGGCTGGGCGCCTATGATACAACCGTTGGAATAACTGCCGATAGCCTGCGATTTCCCGCCTTCTGCGGGAATCGGACGTTTAATTTGTTGCCAATCTTGCGGCGCGGCAAAAGCGTTGGCGGCGTAAAAAACGCTGCTGAAAACGACCGCACTTTTGATGAATTGGAGAAAGGTTTTATTCATGATGGCATCCGTTTTTCAGGTTTATCGGCATTGCGCTTTa                                                                                 >  1:365863/1‑251 (MQ=255)                                                                                                                                                   cTTGATAGCCGTCGCCTTTATAAGGCAAAGGCTGGGCGCCTATGATACAACCGTTGGAATAACTGCCGATAGCCTGCGATTTCCCGCCTTCTGCGGGAATCGGACGTTTAATTTGTTGCCAATCTTGCGGCGCGGCAAAAGCGTTGGCGGCGTAAAAAACGCTGCTGAAAACGACCGCACTTTTGATGAATTGGAGAAAGGTTTTATTCATGATGGCATCCGTTTTTCAGGTTTATCGGCATTGCGCTTTa                                                                                 >  1:336692/1‑251 (MQ=255)                                                                                                                                                   cTTGATAGCCGTCGCCTTTATAAGGCAAAGGCTGGGCGCCTATGATACAACCGTTGGAATAACTGCCGATAGCCTGCGATTTCCCGCCTTCTGCGGGAATCGGACGTTTAATTTGTTGCCAATCTTGCGGCGCGGCAAAAGCGTTGGCGGCGTAAAAAACGCTGCTGAAAACGACCGCACTTTTGATGAATTGGAGAAAGGTTTTATTCATGATGGCATCCGTTTTTCAGGTTTATCGGCATTGCGCTTTa                                                                                 >  1:528433/1‑251 (MQ=255)                                                                                                                                                   cTTGATAGCCGTCGCCTTTATAAGGCAAAGGCTGGGCGCCTATGATACAACCGTTGGAATAACTGCCGATAGCCTGCGATTTCCCGCCTTCTGCGGGAATCGGACGTTTAATTTGTTGCCAATCTTGCGGCGCGGCAAAAGCGTTGGCGGCGTAAAAAACGCTGCTGAAAACGACCGCACTTTTGATGAATTGGAGAAAGGCTTTATTCATGATGGCATCCGTTTTTCAGGTTTATCGGCATTGCGCTTTa                                                                                 >  1:525618/1‑251 (MQ=255)                                                                                                                                                     tGATAGCCGTCGCCTTTATAAGGCAAAGGCTGGGCGCCTATGATACAACCGTTGGAATAACTGCCGATAGCCTGCGATTTCCCGCCTTCTGCGGGAATCGGACGTTTAATTTGTTGCCAATCTTgcgg                                                                                                                                                                                                          >  1:358595/1‑128 (MQ=255)                                                                                                                                                               cGCCTTTATAAGGCAAAGGCTGGGCGCCTATGATACAACCGTTGGAATAACTGCCGATAGCCTGCGATTTCCCGCCTTCTGTGGGAATCGGACGTTTAATTTGTTGCCAATCTTGCGGCGCGGCAAAAGCGTTGGCGGCGTAAAAAACGCTGCTGAAAACGACCGCACTTTTGATGAATTGGAGAAAGGTTTTATTCATGATGGCATCCGTTTTTCAGGTTTATCGGCATTGCGCTTTAAAACGCAACaaa                                                                     <  1:447926/251‑1 (MQ=255)                                                                                                                                                                 ccTTTATAAGGCAAAGGCTGGGCGCCTATGATACAACCGTTGGAATAACTTCCGATAGCCTGCGATTTCCCGCCTTCTGCGGGAATCGGACGTTTAATTTGTTGCCAATCTTGCGGCGCGGCAAAAGCGTTGGCGGCGTAAAAAACGCTGCTGAAAACGACCGCACTTTTGATGAATTGgag                                                                                                                                        >  1:318119/1‑182 (MQ=255)                                                                                                                                                                 ccTTTATAAGGCAAAGGCTGGGCGCCTATGATACAACCGTTGGAATAACTGCCGATAGCCTGCGATTTCCCGCCTTCTGCGGGAATCGGACGTTTAATTTGTTGCCAATCTTGCGGCGCGGCAAAAGCGTTGGCGGCGTAAAAAACGCTGCTGAAAACGACCGCACTTTTGATGAATTGGAGAAAGGTTTTATTCATGATGGCATCCGTTTTTCAGGTTTATCGGCATTGCGCTTTAAAACGCAACAAATg                                                                   >  1:418011/1‑251 (MQ=255)                                                                                                                                                                    ttATAAGGCAAAGGCTGGGCGCCTATGATACAACCGTTGGAATAACTGCCGATAGCCTGCGATTTCCCGCCTTCTGCGGGAATCGGACGTTTAATTTGTTGCCAATCTTGCGGCGCGGCAAAAGCGTTGGCGGCGTAAAAAACGCTGCTGAAAACGACCGCACTTTTGATGAATTGGAGAAAGGTTTTATTCATGATGGCATCCGTTTTTc                                                                                                        <  1:253067/211‑1 (MQ=255)                                                                                                                                                                    ttATAAGGCAAAGGCTGGGCGCCTATGATACAACCGTTGGAATAACTGCCGATAGCCTGCGATTTCCCGCCTTCTGCGGGAATCGGACGTTTAATTTGTTGCCAATCTTGCGGCGCGGCAAAAGCGTTGGCGGCGTAAAAAACGCTGCTGAAAACGACCGCACTTTTGATGAATTGGAGAAAGGTTTTATTCATGATGGCATCCGTTTTTCAGGTTTATCGGCATTGCGCTTTAAAACGCAACAAATGAt                                                                 >  1:372085/1‑250 (MQ=255)                                                                                                                                                                     tataAGGCAAAGGCTGGGCGCCTATGATACAACCGTTGGAATAACTGCCGATAGCCTGCGATTTCCCGCCTTCTGCGGGAATCGGACGTTTAATTTGTTGCCAATCTTGCGGCGCGGCAAAAGCGTTGGCGGCGTAAAAAACGCTGCTGAAAACGACCGCACTTTTGATGAATTGGAGAAAGGTTTTATTCATGATGGCATCCGTTTTTCAGGTTTATCGGCATTGCGCTTTAAAACGCAACAAATGATcc                                                               <  1:64401/251‑1 (MQ=255)                                                                                                                                                                        aaGGCAAAGGCTGGGCGCCTATGATACAACCGTTGGAATAACTGCCGATAGCCTGCGATTTCCCGCCTTCTGCGGGAATCGGACGTTTAATTTGTTGCCAATCTTGCGGCGCGGCAAAAGCGTTGGCGGCGTAAAAAACGCTGCTGAAAACGACCGCACTTTTGATGAATTGGAGAAAGGTTTTATTCATGATGGCATCCGTTTTTCAGGTTTATCGGCATTGCGCTTTAAAACGCAACAAATGATCcaac                                                            <  1:725577/251‑1 (MQ=255)                                                                                                                                                                        aaGGCAAAGGCTGGGCGCCTATGATACAACCGTTGGAATAACTGCCGATAGCCTGCGATTTCCCGCCTTCTGCGGGAATCGGACGTTTAATTTGTTGCCAATCTTGCGGCGCGGCAAAAGCGTTGGCGGCGTAAAAAACGCTGCTGAAAACGACCGCACTTTTGATGAATTGGAGAAAGGTTTTATTCATGATGGCATCCGTTTTTCAGGTTTATCGGCATTGCGCTTTAAAACGCAACAAATGATCcaac                                                            <  1:117322/251‑1 (MQ=255)                                                                                                                                                                          ggCAAAGGCTGGGCGCCTATGATACAACCGTTGGAATAACTGCCGATAGCCTGCGATTTCCCGCCTTCTGCGGGAATCGGACGTTTAATTTGTTGCCAATCTTGCGGCGCGGCAAAAg                                                                                                                                                                                               >  1:793404/1‑118 (MQ=255)                                                                                                                                                                          ggCAAAGGCTGGGCGCCTATGATACAACCGTTGGAATAACTGCCGATAGCCTGCGATTTCCCGCCTTCTGCGGGAATCGGACGTTTAATTTGTTGCCAATCTTGCGGCGCGGCAAAAGCGTTGGCGGCGTAAAAAACGCTGCTGAAAACGACCGCACTTTTGATGaa                                                                                                                                              >  1:174434/1‑167 (MQ=255)                                                                                                                                                                          ggCAAAGGCTGGGCGCCTATGATACAACCGTTGGAATAACTGCCGATAGCCTGCGATTTCCCGCCTTCTGCGGGAATCGGACGTTTAATTTGTTGCCAATCTTGCGGCGCGGCAAAAGCGTTGGCGGCGTAAAAAACGCTGCTGAAAACGACCGCACTTTTGATGAATTGGAGAAAGGTTTTATTCATGATGGCATCCGTTTTTCAGGTTTATCGGCATTGCGCTTTAAAAc                                                                             >  1:622809/1‑232 (MQ=255)                                                                                                                                                                          ggCAAAGGCTGGGCGCCTATGATACAACCGTTGGAATAACTGCCGATAGCCTGCGATTTCCCGCCTTCTGCGGGAATCGGACGTTTAATTTGTTGCCAATCTTGCGGCGCGGCAAAAGCGTTGGCGGCGTAAAAAACGCTGCTGAAAACGACCGCACTTTTGATGAATTGGAGAAAGGTTTTATTCATGATGGCATCCGTTTTTCAGGTTTATCGGCATTGCGCTTTAAAACGCAACAAATGATCcaacaa                                                          >  1:637525/1‑251 (MQ=255)                                                                                                                                                                            cAAAGGCTGGGCGCCTATGATACAACCGTTGGAATAACTGCCGATAGCCTGCGATTTCCCGCCTTCTGCGGGAATCGGACGTTTAATTTGTTGCCAATCTTGCGGCGCGGCAAAAGCGTTGGCGGCGTAAAAAACGCTGCTGAAAACGACCGCACTTTTGATGAATTGGAGAAAGGTTTTATTCATGATGGCATCCGTTTTTCAGGTTTATCGGCATTGCGCTTTAAAACGCAACAAATGATCCAacaat                                                         <  1:788879/250‑1 (MQ=255)                                                                                                                                                                             aaaGGCTGGGCGCCTATGATACAACCGTTGGAATAACTGCCGATAGCCTGCGATTTCCCGCCTTCTGCGGGAATCGGACGTTTAATTTGTTGCCAATCTTGCGGCGCGGCAAAAGCGTTGGCGGCGTAAAAAACGCTGCTGAAAACGACCGCACTTTTGATGAATTGGAGAAAGGTTTTATTCATGATGGCATCCGTTTTTCAGGTTTATCGGCATTGCGCTTTAAAACGCAACAAATGATCCAacaatac                                                       <  1:786344/251‑1 (MQ=255)                                                                                                                                                                             aaaGGCTGGGCGCCTATGATACAACCGTTGGAATAACTGCCGATAGCCTGCGATTTCCCGCCTTCTGCGGGAATCGGACGTTTAATTTGTTGCCAATCTTGCGGCGCGGCAAAAGCGTTGGCGGCGTAAAAAACGCTGCTGAAAACGACCGCACTTTTGATGAATTGGAGAAAGGTTTTATTCATGATGGCATCCGTTTTTCAGGTTTATCGGCATTGCGCTTTAAAACGCAACAAATGATCCAacaatac                                                       <  1:87776/251‑1 (MQ=255)                                                                                                                                                                             aaaGGCTGGGCGCCTATGATACAACCGTTGGAATAACTGCCGATAGCCTGCGATTTCCCGCCTTCTGCGGGAATCGGACGTTTAATTTGTTGCCAATCTTGCGGCGCGGCAAAAGCGTTGGCGGCGTAAAAAACGCTGCTGAAAACGACCGCACTTTTGATGAATTGGAGAAAGGTTTTATTCATGATGGCATCCGTTTTTCAGGTTTATCGGCATTGCGCTTTAAAACGCAACAAATGATCCAacaatac                                                       <  1:676429/251‑1 (MQ=255)                                                                                                                                                                               aGGCTGGGCGCCTATGATACAACCGTTGGAATAACTGCCGATAGCCTGCGATTTCCCGCCTTCTGCGGGAATCGGACGTTTAATTTGTTGCCAATCTTGCGGCGCGGCAAAAGCGTTGGCGGCGTAAAAAACGCTGCTGAAAACGACCGCACTTTTGATGAATTGGAGAAAGGTTTTATTCATGATGGCATCCGTTTTTCAGGTTTATCGGCATTGCGCTTTAAAACGCAACAAATGATCCAacaatacaa                                                     >  1:783478/1‑251 (MQ=255)                                                                                                                                                                                 gCTGGGCGCCTATGATACAACCGTTGGAATAACTGCCGATAGCCTGCGATTTCCCGCCTTCTGCGGGAATCGGACGTTTAATTTGTTGCCAATCTTGCGGCGCGGCAAAAGCGTTGGCGGCGTAAAAAACGCTGCTGAAAACGACCGCACTTTTGATGAATTGGAGAAAGGTTTTATTCATGATGGCATCCGTTTTTCAGGTTTATCGGCATTGCGCTTTAAAACGCAACAAATGATCCAacaatacaat                                                    <  1:692853/250‑1 (MQ=255)                                                                                                                                                                                         ccTATGATACAACCGTTGGAATAACTGCCGATAGCCTGCGATTTCCCGCCTTCTGCGGGAATCGGACGTTTAATTTGTTGCCAATCTTGCGGCGCGGCAAAAGCGTTGGCGGCGTAAAAAACGCTGCTGAAAACGACCGCACTTTTGATGAATTGGAGAAAGGTTTTATTCATGATGGCATCCGTTTTTCAGGTTTATCGGCATTGCGCTTTAAAACGCAACAAATg                                                                   <  1:257509/227‑1 (MQ=255)                                                                                                                                                                                          cTATGATACAACCGTTGGAATAACTGCCGATAGCCTGCGATTTCCCGCCTTCTGCGGGAATCGGACGTTTAATTTGTTGCCAATCTTGCGGCGCGGCAAAAGCGTTGGCGGCGTAAAAAACgc                                                                                                                                                                          <  1:45301/123‑1 (MQ=255)                                                                                                                                                                                             tGATACAACCGTTGGAATAACTGCCGATAGCCTGCGATTTCCCGCCTTCTGCGGGAATCGGACGTTTAATTTGTTGCCAATCTTGCGGCGCGGCAAAAGCGTTGGCGGCGTAAAAAACGCTGCTGAAAACGACCGCACTTTTGATGAATTGGAGAAAGGTTTTATTCATGATGGCATCCGTTTTTCAGGTTTATCGGCATTGCGCTTTAAAACGCAACAAATGATCCAacaatac                                                       <  1:528913/235‑1 (MQ=255)                                                                                                                                                                                             tGATACAACCGTTGGAATAACTGCCGATAGCCTGCGATTTCCCGCCTTCTGCGGGAATCGGACGTTTAATTTGTTGCCAATCTTGCGGCGCGGCAAAAGCGTTGGCGGCGTAAAAAACGCTGCTGAAAACGACCGCACTTTTGATGAATTGGAGAAAGGTTTTATTCATGATGGCATCCGTTTTTCAGGTTTATCGGCATTGCGCTTTAAAACGCAACAAATGATCCAACAATACAATCGCCGTCATAg                                         >  1:846258/1‑249 (MQ=255)                                                                                                                                                                                             tGATACAACCGTTGGAATAACTGCCGATAGCCTGCGATTTCCCGCCTTCTGCGGGAATCGGACGTTTAATTTGTTGCCAATCTTGCGGCGCGGCAAAAGCGTTGGCGGCGTAAAAAACGCTGCTGAAAACGACCGCACTTTTGATGAATTGGAGAAAGGTTTTATTCATGATGGCATCCGTTTTTCAGGTTTATCGGCATTGCGCTTTAAAACGCAACAAATGATCCAACAATACAATCGCCGTCATAg                                         <  1:506465/249‑1 (MQ=255)                                                                                                                                                                                             tGATACAACCGTTGGAATAACTGCCGATAGCCTGCGATTTCCCGCCTTCTGCGGGAATCGGACGTTTAATTTGTTGCCAATCTTGCGGCGCGGCAAAAGCGTTGGCGGCGTAAAAAACGCTGCTGAAAACGACCGCACTTTTGATGAATTGGAGAAAGGTTTTATTCATGATGGCATCCGTTTTTCAGGTTTATCGGCATTGCGCTTTAAAACGCAACAAATGATCCAACAATACAATCGCCGTCATAGCt                                       <  1:684759/251‑1 (MQ=255)                                                                                                                                                                                             tGATACAACCGTTGGAATAACTGCCGATAGCCTGCGATTTCCCGCCTTCTGCGGGAATCGGACGTTTAATTTGTTGCCAATCTTGCGGCGCGGCAAAAGCGTTGGCGGCGTAAAAAACGCTGCTGAAAACGACCGCACTTTTGATGAATTGGAGAAAGGTTTTATTCATGATGGCATCCGTTTTTCAGGTTTATCGGCATTGCGCTTTAAAACGCAACAAATGATCCAACAATACAATCGCCGTCATAGCt                                       <  1:393442/251‑1 (MQ=255)                                                                                                                                                                                             tGATACAACCGTTGGAATAACTGCCGATAGCCTGCGATTTCCCGCCTTCTGCGGGAATCGGACGTTTAATTTGTTGCCAATCTTGCGGCGCGGCAAAAGCGTTGGCGGCGTAAAAAACGCTGCTGAAAACGACCGCACTTTTGATGAATTGGAGAAAGGTTTTATTCATGATGGCATCCGTTTTTCAGGTTTATCGGCATTGCGCTTTAAAACGCAACAAATGATCCAACAATACAATCGCCGTCATAGCt                                       <  1:59162/251‑1 (MQ=255)                                                                                                                                                                                               aTACAACCGTTGGAATAACTGCCGATAGCCTGCGATTTCCCGCCTTCTGCGGGAATCGGACGTTTAATTTGTTGCCAATCTTGCGGCGCGGCAAAAGCGTTGGCGGCGTAAAAAACGCTGCTGAAAACGACCGCACTTTTGATGAATTGGAGAAAGGTTTTATTCATGATGGCATCCGTTTTTCAGGTTTATCGGCATTGCGCTTTAAAACGCAACAAATGATCCAACAATACAATCGCCGTCATAGCtt                                      >  1:97628/1‑250 (MQ=255)                                                                                                                                                                                               aTACAACCGTTGGAATAACTGCCGATAGCCTGCGATTTCCCGCCTTCTGCGGGAATCGGACGTTTAATTTGTTGCCAATCTTGCGGCGCGGCAAAAGCGTTGGCGGCGTAAAAAACGCTGCTGAAAACGACCGCACTTTTGATGAATTGGAGAAAGGTTTTATTCATGATGGCATCCGTTTTTCAGGTTTATCGGCATTGCGCTTTAAAACGCAACAAATGATCCAACAATACAATCGCCGTCATAGCtt                                      >  1:68599/1‑250 (MQ=255)                                                                                                                                                                                               aTACAACCGTTGGAATAACTGCCGATAGCCTGCGATTTCCCGCCTTCTGCGGGAATCGGACGTTTAATTTGTTGCCAATCTTGCGGCGCGGCAAAAGCGTTGGCGGCGTAAAAAACGCTGCTGAAAACGACCGCACTTTTGATGAATTGGAGAAAGGTTTTATTCATGATGGCATCCGTTTTTCAGGTTTATCGGCATTGCGCTTTAAAACGCAACAAATGATCCAACAATACAATCGCCGTCATAGCtt                                      >  1:839605/1‑250 (MQ=255)                                                                                                                                                                                               aTACAACCGTTGGAATAACTGCCGATAGCCTGCGATTTCCCGCCTTCTGCGGGAATCGGACGTTTAATTTGTTGCCAATCTTGCGGCGCGGCAAAAGCGTTGGCGGCGTAAAAAACGCTGCTGAAAACGACCGCACTTTTGATGAATTGGAGAAAGGTTTTATTCATGATGGCATCCGTTTTTCAGGTTTATCGGCATTGCGCTTTAAAACGCAACAAATGATCCAACAATACAATCGCCGTCATAGCt                                       >  1:172935/1‑249 (MQ=255)                                                                                                                                                                                                    aCCGTTGGAATAACTGCCGATAGCCTGCGATTTCCCGCCTTCTGCGGGAATCGGACGTTTAATTTGTTGCCAATCTTGCGGTGCGGCAAAAGCGTTGGCGGCGTAAAAAACGCTGCTGAAAACGACCGCACTTTTGATGAATTGGAGAAAGGTTTTATTCATGATGGCATCCGTTTTTCAGGTTTATCGGCATTGCGCTTTAAAACGCAACAAATGATCCAACAATACAATCGCCGTCATAGCTTCCGCaa                                <  1:436877/251‑1 (MQ=255)                                                                                                                                                                                                    aCCGTTGGAATAACTGCCGATAGCCTGCGATTTCCCGCCTTCTGCGGGAATCGGACGTTTAATTTGTTGCCAATCTTGCGGCGCGGCAAAAGCGTTGGCGGCGTAAAAAACGCTGCTGAAAACGACCGCACTTTTGATGAATTGGAGAAAGGTTTTATTCATGATGGCATCCGTTTTTCAGGTTTATCGGCATTGCGCTTTAAAAc                                                                             <  1:563841/206‑1 (MQ=255)                                                                                                                                                                                                     ccGTTGGAATAACTGCCGATAGCCTGCGATTTCCCGCCTTCTGCGGGAATCGGACGTTTAATTTGTTGCCAATCTTGCGGCGCGGCAAAAGCGTTGGCGGCGTAAAAAACGCTGCTGAAAACGACCGCACTTTTGATGAATTGGAGAAAGGTTTTATTCATGATGGCATCCGTTTTTCAGGTTTATCGGCATTGCGCTTTAAAACGCAACAAATGATCCAACAATACAATCg                                                  <  1:504324/232‑1 (MQ=255)                                                                                                                                                                                                      cGTTGGAATAACTGCCGATAGCCTGCGATTTCCCGCCTTCTGCGGGAATCGGACGTTTAATTTGTTGCCAATCTTGCGGCGCGGCAAAAGCGTTGGCGGCGTAAAAAACGCTGCTGAAAACGACCGCACTTTTGATGAATTGGAGAAAGGTTTTATTCatgat                                                                                                                      <  1:480731/163‑1 (MQ=255)                                                                                                                                                                                                          ggAATAACTGCCGATAGCCTGCGATTTCCCGCCTTCTGCGGGAATCGGACGTTTAATTTGTTGCCAATCTTGCGGCGCGGCAAAAGCGTTGGCGGCGTAAAAAACGCTGCTGAAAACGACCGCACTTTTGATGAATTGGAGAAAGGTTTTATTCATGATGGCATCCGTTTTTCAGGTTTATCGGCATTGCGCTTTAAAACGCAACAAATGATCCAACAATACAATCGCCGTCATAGCTTCCGCAATCGGCa                          >  1:570219/1‑251 (MQ=255)                                                                                                                                                                                                                   gCCGATAGCCTGCGATTTCCCGCCTTCTGCGGGAATCGGACGTTTAATTTGTTGCCAATCTTGCGGCGCGGCAAAAGCGTTGGCGGCGTAAAAAACGCTGCTGAAAACGACCGCACTTTTGATGAATTGGAGAAAGGTTTTATTCATGATGGCATCCGTTTTTCAGGTTTATCGGCATTGCGCTTTAAAAc                                                                             <  1:318874/191‑1 (MQ=255)                                                                                                                                                                                                                   gCCGATAGCCTGCGATTTCCCGCCTTCTGCGGGAATCGGACGTTTAATTTGTTGCCAATCTTGCGGCGCGGCAAAAGCGTTGGCGGCGTAAAAAACGCTGCTGAAAACGACCGCACTTTTGATGAATTGGAGAAAGGTTTTATTCATGATGGCATCCGTTTTTCAGGTTTATCGGCATTGCGCTTTAAAACGCAACAAATGATCCAACAATACAATCGCCGTCATAGCTTCCGCAATCGGCACGGCGCGGa                 >  1:651263/1‑251 (MQ=255)                                                                                                                                                                                                                          gCCTGCGATTTCCCGCCTTCTGCGGGAATCGGACGTTTAATTTGTTGCCAATCTTGCGGCGCGGCAAAAGCGTTGGCGGCGTAAAAAACGCTGCTGAAAACGACCGCACTTTTGATGAATTGGAGAAAGGTTTTATTCATGATGGCATCCGTTTTTCAGGTTTATCGGCATTGCGCTTTAAAACGCAACAAATGATCCAACAATACAATCGCCGTCATAGCTTCCGCAATCGGCACGGCGCGGATGCCGa           >  1:582484/1‑250 (MQ=255)                                                                                                                                                                                                                               cGATTTCCCGCCTTCTGCGGGAATCGGACGTTTAATTTGTTGCCAATCTTGCGGCGCGGCAAAAGCGTTGGCGGCGTAAAAAACGCTGCTGAAAACGACCGCACTTTTGATGAATTGGAGAAAGGTTTTATTCATGATGGCATCCGTTTTTCAGGTTTATCGGCATTGCGCTTTAAAACGCAACAAATGATCCAACAATACAATCGCCGTCATAGCTTCCGCAATCGGCACGGCGCGGATGCCGACACaa      <  1:832701/250‑1 (MQ=255)                                                                                                                                                                                                                                  tttCCCGCCTTCTGCGGGAATCGGACGTTTAATTTGTTGCCAATCTTGCGGCGCGGCAAAAGCGTTGGCGGCGTAAAAAACGCTGCTGAAAACGACCGCACTTTTGATGAATTGGAGAAAGGTTTTATTCATGATGGCATCCGTTTTTCAGGTTTATCGGCATTGCGCTTTAAAACGCAACAAATGATCCAACAATACAATCGCCGTCATAGCTTCCGCAATCGGCACGGCGCGGATGCCGACACAAGGAt  <  1:595577/251‑1 (MQ=255)                                                                                                                                                                                                                                  tttCCCGCCTTCTGCGGGAATCGGACGTTTAATTTGTTGCCAATCTTGCGGCGCGGCAAAAGCGTTGGCGGCGTAAAAAACGCTGCTGAAAACGACCGCACTTTTGATGAATTGGAGAAAGGTTTTATTCATGATGGCATCCGTTTTTCAGGTTTATCGGCATTGCGCTTTAAAACGCAACAAATGATCCAACAATACAATCGCCGTCATAGCTTCCGCAATCGGCACGGCGCGGATGCCGACACAAGGAt  <  1:28825/251‑1 (MQ=255)                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                    GTCAGAAAACGTCCGCCGCCCGGCATAGCAATATCGCCCACCAACATGGTCGGCAAACCCGCCGCCTTGGCGCGTTGCCCCAAACGTTGCAAGTAGCTAATCATGTCAGGGTGTCCGTAATAGCGGTTTTTATTCA‑TGCGAATCACTTGATAGCCGTCGCCTTTATAAGGCAAAGGCTGGGCGCCTATGATACAACCGTTGGAATAACTGCCGATAGCCTGCGATTTCCCGTCTTCTGCGGGAATCGGACGTTTAATTTGTTGCCAATCTTGCGGCGCGGCAAAAGCGTTGGCGGCGTAAAAAACGCTGCTGAAAACGACCGCACTTTTGATGAATTGGAGAAAGGTTTTATTCATGATGGCATCCGTTTTTCAGGTTTATCGGCATTGCGCTTTAAAACGCAACAAATGATCCAACAATACAATCGCCGTCATAGCTTCCGCAATCGGCACGGCGCGGATGCCGACACAAGGAT  >  1/1741572‑1742046

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑