Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,730,391	G→A	intergenic (+10433/‑64451)	unknown → / → unknown	tRNA‑Asn‑GTT/tRNA‑Leu‑CAA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,730,391	0	G	A	100.0%	201.6 / NA	75	intergenic (+10433/‑64451)	unknown/unknown	tRNA‑Asn‑GTT/tRNA‑Leu‑CAA
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (39/36);  total (39/36)

AAACAAGATAGAAAGAATTTTATTCACTACTGAGTGTAACTAAGCCGGCACGTTGTATATATCCATAAATGGAAATATCAATAGATTCAATCAGGTGGGCATATTACACAAATCTGCTACAATGTCCCTGATGGTTATTTATACCATCATTAAGCAGGCAAAAATTGTAATTGTCATTTTATTCAAGGAGTTAATATGAAACTATATTTTAAGCCGGGTGCATGCTCATTCGTACCGCATTTCGCATTGGAAAAAATCAATCAGCCTTATGAAGCCGAAGCAGTGACACAAGAGTATATGAAGAGCCCGGAATACTTAGAGCTCAATCCACAAGGTGCAGTTCCTCTACTCGTTGACGGTAATCTTGTACTTTCCCAAAACTTGGCGATTTTAACTTATTTAGACGATCTTTACCCACAGGCAAAACTCTTTGGCAGCGACTCAGCCAAAGGCAAAGCT  >  1/1730179‑1730637                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                       aaaCAAGATAGAAAGAATTTTATTCACTACTGAGTGTAACTAAGCCGGCACGTTGTATATATCCATAAATGGAAATATCAATAGATTCAATCAGGTGGGCATATTACACAAATCTGCTACAATGTCCCTGATGGTTATTTATACCATCATTAAGCAGGCAAAAATTGTAATTGTCATTTTATTCAAGGAGTTAATATGAAACTATATTTTAAACCGGGTGCATGCTCATTCGTACCGCATTTCGCATTGGa                                                                                                                                                                                                                  >  1:761467/1‑251 (MQ=255) aaaCAAGATAGAAAGAATTTTATTCACTACTGAGTGTAACTAAGCCGGCACGTTGTATATATCCATAAATGGAAATATCAATAGATTCAATCAGGTGGGCATATTACACAAATCTGCTACAATGTCCCTGATGGTTATTTATACCATCATTAAGCAGGCAAAAATTGTAATTGTCATTTTATTCAAGGAGTTAATATGAAACTATATTTTAAACCGGGTGCATGCTCATTCGTACCGCATTTCGCATTGGa                                                                                                                                                                                                                  >  1:19953/1‑251 (MQ=255)                 aTTTTATTCACTACTGAGTGTAACTAAGCCGGCACGTTGTATATATCCATAAATGGAAATATCAATAGATTCAATCAGGTGGGCATATTACACAAATCTGCTACAATGTCCCTGATGGTTATTTATACCATCATTAAGCAGGCAAAAATTGTAATTGTCATTTTATTCAAGGAGTTAATATGAAACTATATTTTAAACCGGGTGCATGc                                                                                                                                                                                                                                            >  1:297504/1‑209 (MQ=255)                 aTTTTATTCACTACTGAGTGTAACTAAGCCGGCACGTTGTATATATCCATAAATGGAAATATCAATAGATTCAATCAGGTGGGCATATTACACAAATCTGCTACAATGTCCCTGATGGTTATTTATACCATCATTAAGCAGGCAAAAATTGTAATTGTCATTTTATTCAAGGAGTTAATATGAAACTATATTTTAAACCGGGTGCATGCTCATTCGTACCGCATTTCGCATTGGAAAAAATCAATCAGc                                                                                                                                                                                                    >  1:683850/1‑249 (MQ=255)                      aTTCACTACTGAGTGTAACTAAGCCGGCACGTTGTATATATCCATAAATGGAAATATCAATAGATTCAATCAGGTGGGCATATTACACAAATCTGCTACAATGTCCCTGATGGTTATTTATACCATCATTAAGCAGGCAAAAATTGTAATTGTCATTTTATTCAAGGAGTTAATATGAAACTATATTTTAAACCGGGTGCATGCTCATTCGt                                                                                                                                                                                                                                    <  1:345797/212‑1 (MQ=255)                                  gtgtAACTAAGCCGGCACGTTGTATATATCCATAAATGGAAATATCAATAGATTCAATCAGGTGGGCATATTACACAAATCTGCTACAATGTCCCTGATGGTTATTTATACCATCATTAAGCAGGCAAAAATTGTAATTGTCATTTTATTCAAGGAGTTAATATGAAACTATATTTTAAACCGGGTGCATGCTCATTCGTACCGCATTTCGCATTGGAAAAAATCAATCAGCCTTATGAAGCCGAAGCAGt                                                                                                                                                                                 >  1:476178/1‑251 (MQ=255)                                  gtgtAACTAAGCCGGCACGTTGTATATATCCATAAATGGAAATATCAATAGATTCAATCAGGTGGGCATATTACACAAATCTGCTACAATGTCCCTGATGGTTATTTATACCATCATTAAGCAGGCAAAAATTGTAATTGTCATTTTATTCAAGGAGTTAATATGAAACTATATTTTAAACCGGGTGCATGCTCATTCGTACCGCATTTCGCATTGGAAAAAATCAATCAGCCTTATGAAGCCGAAGCAGt                                                                                                                                                                                 >  1:412992/1‑251 (MQ=255)                                   tgtAACTAAGCCGGCACGTTGTATATATCCATAAATGGAAATATCAATAGATTCAATCAGGTGGGCATATTACACAAATCTGCTACAATGTCCCTGATGGTTATTTATACCATCATTAAGCAGGCAAAAATTGTAATTGTCATTTTATTCAAGGAGTTAATATGAAACTATATTTTAAACCGGGTGCATGCTCATTCGTACCGCATTTCGCATTGGAAAAAATCAATCAGCCTTATGAAGCCGAAGCAGTg                                                                                                                                                                                <  1:887872/251‑1 (MQ=255)                                           aGCCGGCACGTTGTATATATCCATAAATGGAAATATCAATAGATTCAATCAGGTGGGCATATTACACAAATCTGCTACAATGTCCCTGATGGTTATTTATACCATCATTAAGCAGGCAAAAATTGTAATTGTCATTTTATTCAAGGAGTTAATATGAAACTATATTTTAAACCGGGTGCATGCTCATTCGTACCGCATTTCGCATTGGAAAAAATCAATCAGCCTTATGAAGCCGAAGCAGTGACACAaga                                                                                                                                                                        >  1:340822/1‑251 (MQ=255)                                             ccGGCACGTTGTATATATCCATAAATGGAAATATCAATAGATTCAATCAGGTGGGCATATTACACAAATCTGCTACAATGTCCCTGATGGTTATTTATACCATCATTAAGCAGGCAAAAATTGTAATTGTCATTTTATTCAAGGAGTTAATATGAAACTATATTTTAAACCGGGTGCATGCTCATTCGTACCGCATTTCGCATTGGAAAAAATCAATCAGCCTTATGAAGCCGAAGCAGTGACACAAGAGt                                                                                                                                                                      <  1:479174/251‑1 (MQ=255)                                                 cACGTTGTATATATCCATAAATGGAAATATCAATAGATTCAATCAGGTGGGCATATTACACAAATCTGCTACAATGTCCCTGATGGTTATTTATACCATCATTAAGCAGGCAAAAATTGTAATTGTCATTTTATTCAAGGAGTTAATATGAAACTATATTTTAAACCGGGTGCATGCTCATTCGTACCGCATTTCGCATTGGAAAAAATCAATCAGCCTTATGAAGCCGAAGCAGTGACACAAGAGTatat                                                                                                                                                                  <  1:401141/251‑1 (MQ=255)                                                  aCGTTGTATATATCCATAAATGGAAATATCAATAGATTCAATCAGGTGGGCATATTACACAAATCTGCTACAATGTCCCTGATGGTTATTTATACCATCATTAAGCAGGCAAAAATTGTAATTGTCATTTTATTCAAGGAGTTAATATGAAACTATATTTTAAACCGGGTGCATGCTCATTCGTACCGCATTTCGCATTGGAAAAAATCAATCAGCCTTATGAAGCCGAAGc                                                                                                                                                                                    >  1:229500/1‑232 (MQ=255)                                                  aCGTTGTATATATCCATAAATGGAAATATCAATAGATTCAATCAGGTGGGCATATTACACAAATCTGCTACAATGTCCCTGATGGTTATTTATACCATCATTAAGCAGGCAAAAATTGTAATTGTCATTTTATTCAAGGAGTTAATATGAAACTATATTTTAAACCGGGTGCATGCTCATTCGTACCGCATTTCGCATTGGAAAAAATCAATCAGCCTTATGAAGCCGAAGCAGTGACACAagag                                                                                                                                                                       <  1:133980/245‑1 (MQ=255)                                                   cGTTGTATATATCCATAAATGGAAATATCAATAGATTCAATCAGGTGGGCATATTACACAAATCTGCTACAATGTCCCTGATGGTTATTTATACCATCATTAAGCAGGCAAAAATTGTAATTGTCATTTTATTCAAGGAGTTAATATGAAACTATATTTTAAACCGGGTGCATGCTCATTCGTAc                                                                                                                                                                                                                                  <  1:66745/185‑1 (MQ=255)                                                   cGTTGTATATATCCATAAATGGAAATATCAATAGATTCAATCAGGTGGGCATATTACACAAATCTGCTACAATGTCCCTGATGGTTATTTATACCATCATTAAGCAGGCAAAAATTGTAATTGTCATTTTATTCAAGGAGTTAATATGAAACTATATTTTAAACCGGGTGCATGCTCATTCGTACCGCATTTCGCATTGGAAAAAATCAATCAGCCTTATGAAGCCGAAGCAGTGACACAAGAGTATATGa                                                                                                                                                                <  1:655960/251‑1 (MQ=255)                                                    gTTGTATATATCCATAAATGGAAATATCAATAGATTCAATCAGGTGGGCATATTACACAAATCTGCTACAATGTCCCTGATGGTTATTTATACCATCATTAAGCAGGCAAAAATTGTAATTGTCATTTTATTCAAGGAGTTAATATGAAACTATATTTTAAACCGGGTGCATGCTCATTCGTACCg                                                                                                                                                                                                                                >  1:309320/1‑186 (MQ=255)                                                    gTTGTATATATCCATAAATGGAAATATCAATAGATTCAATCAGGTGGGCATATTACACAAATCTGCTACAATGTCCCTGATGGTTATTTATACCATCATTAAGCAGGCAAAAATTGTAATTGTCATTTTATTCAAGGAGTTAATATGAAACTATATTTTAAACCGGGTGCATGCTCATTCGTACCGCATTTCGc                                                                                                                                                                                                                        <  1:356229/194‑1 (MQ=255)                                                    gTTGTATATATCCATAAATGGAAATATCAATAGATTCAATCAGGTGGGCATATTACACAAATCTGCTACAATGTCCCTGATGGTTATTTATACCATCATTAAGCAGGCAAAAATTGTAATTGTCATTTTATTCAAGGAGTTAATATGAAACTATATTTTAAACCGGGTGCATGCTCATTCGTACCGCATTTCGCATTGGAAAAAATCAATCAGCCTTATGAAGCCGAAGCAGTGACACAAGAGTATATGaa                                                                                                                                                               >  1:282123/1‑251 (MQ=255)                                                    gTTGTATATATCCATAAATGGAAATATCAATAGATTCAATCAGGTGGGCATATTACACAAATCTGCTACAATGTCCCTGATGGTTATTTATACCATCATTAAGCAGGCAAAAATTGTAATTGTCATTTTATTCAAGGAGTTAATATGAAACTATATTTTAAACCGGGTGCATGCTCATTCGTACCGCATTTCGCATTGGAAAAAATCAATCAGCCTTATGAAGCCGAAGCAGTGACACAAGAGTATATGaa                                                                                                                                                               >  1:414801/1‑251 (MQ=255)                                                     ttGTATATATCCATAAATGGAAATATCAATAGATTCAATCAGGTGGGCATATTACACAAATCTGCTACAATGTCCCTGATGGTTATTTATACCATCATTAAGCAGGCAAAAATTGTAATTGTCATTTTATTCAAGGAGTTAATATGAAACTATATTTTAAACCGGGTGCATGCTCATTCGTACCGCATTTCGCATTGGAAAAAATCAATCAGCCTTATGAAGCCGAAGCAGTGACACAAGAGTATATGAag                                                                                                                                                              <  1:735159/251‑1 (MQ=255)                                                     ttGTATATATCCATAAATGGAAATATCAATAGATTCAATCAGGTGGGCATATTACACAAATCTGCTACAATGTCCCTGATGGTTATTTATACCATCATTAAGCAGGCAAAAATTGTAATTGTCATTTTATTCAAGGAGTTAATATGAAACTATATTTTAAACCGGGTGCATGCTCATTCGTACCGCATTTCGCATTGGAAAAAATCAATCAGCCTTATGAAGCCGAAGCAGTGACACAAGAGTATATGAag                                                                                                                                                              >  1:823655/1‑251 (MQ=255)                                                       gTATATATCCATAAATGGAAATATCAATAGATTCAATCAGGTGGGCATATTACACAAATCTGCTACAATGTCCCTGATGGTTATTTATACCATCATTAAGCAGGCAAAAATTGTAATTGTCATTTTATTCAAGGAGTTAATATGAAACTATATTTTAAACCGGGTGCATGCTCATTCGTACCGCATTTCGCATTGGAAAAAATCAATCAGCCTTATGAAGCCGAAGCAGTGacac                                                                                                                                                                            >  1:183322/1‑235 (MQ=255)                                                       gTATATATCCATAAATGGAAATATCAATAGATTCAATCAGGTGGGCATATTACACAAATCTGCTACAATGTCCCTGATGGTTATTTATACCATCATTAAGCAGGCAAAAATTGTAATTGTCATTTTATTCAAGGAGTTAATATGAAACTATATTTTAAACCGGGTGCATGCTCATTCGTACCGCATTTCGCATTGGAAAAAATCAATCAGCCTTATGAAGCCGAAGCAGTGACACAAGAGtat                                                                                                                                                                    >  1:147012/1‑243 (MQ=255)                                                       gTATATATCCATAAATGGAAATATCAATAGATTCAATCAGGTGGGCATATTACACAAATCTGCTACAATGTCCCTGATGGTTATTTATACCATCATTAAGCAGGCAAAAATTGTAATTGTCATTTTATTCAAGGAGTTAATATGAAACTATATTTTAAACCGGGTGCATGCTCATTCGTACCGCATTTCGCATTGGAAAAAATCAATCAGCCTTATGAAGCCGAAGCAGTGACACAAGAGTATATGAag                                                                                                                                                              <  1:638130/249‑1 (MQ=255)                                                                 aTAAATGGAAATATCAATAGATTCAATCAGGTGGGCATATTACACAAATCTGCTACAATGTCCCTGATGGTTATTTATACCATCATTAAGCAGGCAAAAATTGTAATTGTCATTTTATTCAAGGAGTTAATATGAAACTATATTTTAAACCGGGTGCATGCTCATTCGTACCGCATTTCGCATTGGAAAAAATCAATCAGCCTTATGAAGCCGAAGCAGTGACACAAGAGTATATGAAGAGCCCGGAATAc                                                                                                                                                  <  1:654707/251‑1 (MQ=255)                                                                                    gATTCAATCAGGTGGGCATATTACACAAATCTGCTACAATGTCCCTGATGGTTATTTATACCATCATTAAGCAGGCAAAAATTGTAATTGTCATTTTATTCAAGGAGTTAATATGAAACTATATTTTAAACCGGGTGCATGCTCATTCGTACCg                                                                                                                                                                                                                                <  1:184011/154‑1 (MQ=255)                                                                                    gATTCAATCAGGTGGGCATATTACACAAATCTGCTACAATGTCCCTGATGGTTATTTATACCATCATTAAGCAGGCAAAAATTGTAATTGTCATTTTATTCAAGGAGTTAATATGAAACTATATTTTAAACCGGGTGCATGCTCATTCGTACCGCATTTCGCATTGGAAAAAATCAATCAGCCTTATGAAGCCGAAGCAGTGACACAAGAGTATATGa                                                                                                                                                                >  1:320240/1‑218 (MQ=255)                                                                                     aTTCAATCAGGTGGGCATATTACACAAATCTGCTACAATGTCCCTGATGGTTATTTATACCATCATTAAGCAGGCAAAAATTGTAATTGTCATTTTATTCAAGGAGTTAATATGAAACTATATTTTAAACCGGGTGCATGCTCATTCGTACCGCATTTCGCATTGGAAAAAATCAATCAGCCTTATGAAGCCGAAGCAGTGACACAAGAGTATATGAAGAGCCCGGAATACTTAGAGCTCAATCCACAAgg                                                                                                                              <  1:613101/251‑1 (MQ=255)                                                                                        cAATCAGGTGGGCATATTACACAAATCTGCTACAATGTCCCTGATGGTTATTTATACCATCATTAAGCAGGCAAAAATTGTAATTGTCATTTTATTCAAGGAGTTAATATGAAACTATATTTTAAACCGGGTGCATGCTCATTCGTACCGCATTTCGCATTGGAAAAaat                                                                                                                                                                                                            <  1:421717/170‑1 (MQ=255)                                                                                        cAATCAGGTGGGCATATTACACAAATCTGCTACAATGTCCCTGATGGTTATTTATACCATCATTAAGCAGGCAAAAATTGTAATTGTCATTTTATTCAAGGAGTTAATATGAAACTATATTTTAAACCGGGTGCATGCTCATTCGTACCGCATTTCGCATTGGAAAAAATCAATCAGCCTTATGAAGCCGAAGCAGTGACACAAGAGTATATGAAGAGCCCGGAATACTTAGAGCTCAATCCACAAGGTGc                                                                                                                           <  1:270224/251‑1 (MQ=255)                                                                                         aaTCAGGTGGGCATATTACACAAATCTGCTACAATGTCCCTGATGGTTATTTATACCATCATTAAGCAGGCAAAAATTGTAATTGTCATTTTATTCAAGGAGTTAATATGAAACTATATTTTAAACCGGGTGCATGCTCATTCGTAc                                                                                                                                                                                                                                  >  1:119425/1‑147 (MQ=255)                                                                                            cAGGTGGGCATATTACACAAATCTGCTACAATGTCCCTGATGGTTATTTATACCATCATTAAGCAGGCAAAAATTGTAATTGTCATTTTATTCAAGGAGTTAATATGAAACTATATTTTAAACCGGGTGCATGCTCATTCGTACCGCATTTCGCATTGGAAAAAATCAATCAGCCTTATGAAGCCGAAGCAGTGACACAAGAGTATATg                                                                                                                                                                 <  1:557921/209‑1 (MQ=255)                                                                                                 gggCATATTACACAAATCTGCTACAATGTCCCTGATGGTTATTTATACCATCATTAAGCAGGCAAAAATTGTAATTGTCATTTTATTCAAGGAGTTAATATGAAACTATATTTTAAACCGGGTGCATGCTCATTCGTACCGCATTTCGCATTGGAAAAAATCAATCAGCCTTATGAAGCCGAAGCAGTGACACAAGAGTATATGAAGAGCCCGGAATACTTAGAGCTCAATCCACAAGGTGCAGTTCct                                                                                                                    >  1:481059/1‑249 (MQ=255)                                                                                                 gggCATATTACACAAATCTGCTACAATGTCCCTGATGGTTATTTATACCATCATTAAGCAGGCAAAAATTGTAATTGGCATTTTATTCAAGGAGTTAATATGAAACTATATTTTAAACCGGGTGCATGCTCATTCGTACCGCATTTCGCATTGGAAAAAATCAATCAGCCTTATGAAGCCGAAGCAGTGACACAAGAGTATATGAAGAGCCCGGAATACTTAGAGCTCAATCCACAAGGTGCAGTTc                                                                                                                      <  1:434415/247‑1 (MQ=255)                                                                                                     atatTACACAAATCTGCTACAATGTCCCTGATGGTTATTTATACCATCATTAAGCAGGCAAAAATTGTAATTGTCATTTTATTCAAGGAGTTAATATGAAACTATATTTTAAACCGGGTGCATGCTCATTCGTACCGCATTTCGCATTGGAAAAAATCAATCAGCCTTATGa                                                                                                                                                                                             <  1:215644/172‑1 (MQ=255)                                                                                                     atatTACACAAATCTGCTACAATGTCCCTGATGGTTATTTATACCATCATTAAGCAGGCAAAAATTGTAATTGTCATTTTATTCAAGGAGTTAATATGAAACTATATTTTAAACCGGGTGCATGCTCATTCGTACCGCATTTCGCATTGGAAAAAATCAATCAGCCTTATGAAGCCGAAGCAGTGACACAAGAGTATATGAAGAGCCCGGAATACTTAGAGCTCAATCCACAAGGTGCAGTTCCTCTACt                                                                                                               >  1:473258/1‑250 (MQ=255)                                                                                                          acacAAATCTGCTACAATGTCCCTGATGGTTATTTATACCATCATTAAGCAGGCAAAAATTGTAATTGTCATTTTATTCAAGGAGTTAATATGAAACTATATTTTAAACCGGGTGCATGCTCATTCGTACCGCATTTCGCATTGGAAAAAATCAATCAGCCTTATGAAGCCGAAGCAGTGACACAAGAGTATATGAAGAGCCCgg                                                                                                                                                       <  1:98796/205‑1 (MQ=255)                                                                                                          acacAAATCTGCTACAATGTCCCTGATGGTTATTTATACCATCATTAAGCAGGCAAAAATTGTAATTGTCATTTTATTCAAGGAGTTAATATGAAACTATATTTTAAACCGGGTGCATGCTCATTCGTACCGCATTTCGCATTGGAAAAAATCAATCAGCCTTATGAAGCCGAAGCAGTGACACAAGAGTATATGAAGAGCCCGGAATACTTAGAGc                                                                                                                                           >  1:171819/1‑217 (MQ=255)                                                                                                          acacAAATCTGCTACAATGTCCCTGATGGTTATTTATACCATCATTAAGCAGGCAAAAATTGTAATTGTCATTTTATTCAAGGAGTTAATATGAAACTATATTTTAAACCGGGTGCATGCTCATTCGTACCGCATTTCGCATTGGAAAAAATCAATCAGCCTTATGAAGCCGAAGCAGTGACACAAGAGTATATGAAGAGCCCGGAATACTTAGAGCTCAATCcac                                                                                                                                  >  1:829802/1‑226 (MQ=255)                                                                                                          acacAAATCTGCTACAATGTCCCTGATGGTTATTTATACCATCATTAAGCAGGCAAAAATTGTAATTGTCATTTTATTCAAGGAGTTAATATGAAACTATATTTTAAACCGGGTGCATGCTCATTCGTACCGCATTTCGCATTGGAAAAAATCAATCAGCCTTATGAAGCCGAAGCAGTGACACAAGAGTATATGAAGAGCCCGGAATACTTAGAGCTCAATCCACAAGGTGCAGTTCCTCTACTCGTTGa                                                                                                         >  1:14820/1‑251 (MQ=255)                                                                                                                  cTGCTACAATGTCCCTGATGGTTATTTATACCATCATTAAGCAGGCAAAAATTGTAATTGTCATTTTATTCAAGGAGTTAATATGAAACTATATTTTAAACCGGGTGCATGCTCATTCGTACCGCATTTCGCATTGGAAAAAATCAATCAGCCTTATGAAGCCGAAGCAGTGACACAAGAGTATATGAAGAGCCCGGAATACTTAGAGc                                                                                                                                           >  1:347873/1‑209 (MQ=255)                                                                                                                  cTGCTACAATGTCCCTGATGGTTATTTATACCATCATTAAGCAGGCAAAAATTGTAATTGTCATTTTATTCAAGGAGTTAATATGAAACTATATTTTAAACCGGGTGCATGCTCATTCGTACCGCATTTCGCATTGGAAAAAATCAATCAGCCTTATGAAGCCGAAGCAGTGACACAAGAGTATATGAAGAGCCCGGAATACTTAGAGCTCAATCCACAAGGTGCAg                                                                                                                         >  1:326601/1‑227 (MQ=255)                                                                                                                   tGCTACAATGTCCCTGATGGTTATTTATACCATCATTAAGCAGGCAAAAATTGTAATTGTCATTTTATTCAAGGAGTTAATATGAAACTATATTTTAAACCGGGTGCATGCTCATTCGTACCGCATTTCGCATTGGAAAAAATCAATCAGCCTTATGAAGCCGAAGCAGTGACACAAGAGTATATGAAGAGCCCGGAATACTTAGAGCTCAATCCACAAGGTGCAGTTCCTCTACTCGTTGACGGTAATCt                                                                                                <  1:659438/251‑1 (MQ=255)                                                                                                                    gCTACAATGTCCCTGATGGTTATTTATACCATCATTAAGCAGGCAAAAATTGTAATTGTCATTTTATTCAAGGAGTTAATATGAAACTATATTTTAAACCGGGTGCATGCTCATTCGTACCGCATTTCGCATTGGAAAAAATCAATCAGCCTTATGAAGCCGAAGCAGTGACACAAGAGTATATGAAGAGCCCGGAATACTTAGAGCTCAATCCACAAGGTGCAGt                                                                                                                        <  1:752205/226‑1 (MQ=255)                                                                                                                       aCAATGTCCCTGATGGTTATTTATACCATCATTAAGCAGGCAAAAATTGTAATTGTCATTTTATTCAAGGAGTTAATATGAAACTATATTTTAAACCGGGTGCATGCTCATTCGTACCGCATTTCGCATTGGAAAAAATCAATCAGCCTTATGa                                                                                                                                                                                             <  1:841095/154‑1 (MQ=255)                                                                                                                        cAATGTCCCTGATGGTTATTTATACCATCATTAAGCAGGCAAAAATTGTAATTGTCATTTTATTCAAGGAGTTAATATGAAACTATATTTTAAACCGGGTGCATGCTCATTCGTACCGCATTTCGCATTGGAAAAAATCAATCAGCCTTATGAAGCCGAAGCAGTGACACAAGAGTATATGAAGAGCCCGGAATACTTAGAGc                                                                                                                                           >  1:325160/1‑203 (MQ=255)                                                                                                                        cAATGTCCCTGATGGTTATTTATACCATCATTAAGCAGGCAAAAATTGTAATTGTCATTTTATTCAAGGAGTTAATATGAAACTATATTTTAAACCGGGTGCATGCTCATTCGTACCGCATTTCGCATTGGAAAAAATCAATCAGCCTTATGAAGCCGAAGCAGTGACACAAGAGTATATGAAGAGCCCGGAATACTTAGAGCTCAATCCACAAGGTGCAGTTCCTCTACTCGTTGACGGTAATCTTGTAc                                                                                           <  1:873154/251‑1 (MQ=255)                                                                                                                            gTCCCTGATGGTTATTTATACCATCATTAAGCAGGCAAAAATTGTAATTGTCATTTTATTCAAGGAGTTAATATGAAACTATATTTTAAACCGGGTGCATGCTCATTCGt                                                                                                                                                                                                                                    <  1:620535/110‑1 (MQ=255)                                                                                                                              cccTGATGGTTATTTATACCATCATTAAGCAGGCAAAAATTGTAATTGTCATTTTATTCAAGGAGTTAATATGAAACTATATTTTAAACCGGGTGCATGCTCATTCGTACCGCATTTCGCATTGGAAAAAATCAATCAGCCTTATGAAGCCGAAGCAGTGACACAAGAGTATATGAAGAGCCCGGAATACtt                                                                                                                                                <  1:852398/192‑1 (MQ=255)                                                                                                                                  gATGGTTATTTATACCATCATTAAGCAGGCAAAAATTGTAATTGTCATTTTATTCAAGGAGTTAATATGAAACTATATTTTAAACCGGGTGCATGCTCATTCGTACCGCATTTCGCATTGGAAAAAATCAATCAGCCTTATGAAGCCGAAGCAGTGACACAAGAGTATATGAAGAGCCCGGAATACTTAGAGCTCAATCCACAAGGTGCAGTTCCTCTACTc                                                                                                              <  1:670923/222‑1 (MQ=255)                                                                                                                                        tatttatACCATCATTAAGCAGGCAAAAATTGTAATTGTCATTTTATTCAAGGAGTTAATATGAAACTATATTTTAAACCGGGTGCATGCTCATTCGTACCGCATTTCGCATTGGAAAAAATCAATCAGCCTTATGAAGCCGAAGCAGTGACACAAGAGTATATGAAGAGCCCGGAATACTTAGAGCTCAATCCACAAgg                                                                                                                              >  1:488592/1‑200 (MQ=255)                                                                                                                                         atttatACCATCATTAAGCAGGCAAAAATTGTAATTGTCATTTTATTCAAGGAGTTAATATGAAACTATATTTTAAACCGGGTGCATGCTCATTCGTACCGCATTTCGCATTGGAAAAAATCAATCAGCCTTATGAAGCCGAAGCAGTGACACAAGAGTATATGAAGAGCCCGGAATACTTAGAGCTCAATCCACAAGGTGCAGTTCCTCTACTCGTTGACGGTAATCTTGTACTTTCCCAAAACTTGGCg                                                                          >  1:263095/1‑251 (MQ=255)                                                                                                                                         atttatACCATCATTAAGCAGGCAAAAATTGTAATTGTCATTTTATTCAAGGAGTTAATATGAAACTATATTTTAAACCGGGTGCATGCTCATTCGTACCGCATTTCGCATTGGAAAAAATCAATCAGCCTTATGAAGCCGAAGCAGTGACACAAGAGTATATGAAGAGCCCGGAATACTTAGAGCTCAATCCACAAGGTGCAGTTCCTCTACTCGTTGACGGTAATCTTGTACTTTCCCAAAACTTGGCg                                                                          >  1:457168/1‑251 (MQ=255)                                                                                                                                                  atcatTAAGCAGGCAAAAATTGTAATTGTCATTTTATTCAAGGAGTTAATATGAAACTATATTTTAAACCGGGTGCATGCTCATTCGTACCGCATTTCGCATTGGAAAAAATCAATCAGCCTTATGAAGCCGAAGCAGTGACACAAGAGTATATGAag                                                                                                                                                              >  1:19872/1‑158 (MQ=255)                                                                                                                                                   tcatTAAGCAGGCAAAAATTGTAATTGTCATTTTATTCAAGGAGTTAATATGAAACTATATTTTAAACCGGGTGCATGCTCATTCGTACCGCATTTCGCATTGGAAAAAATCAATCAGCCTTATGAAGCCGAAGCAGTGACACAAGAGTATATg                                                                                                                                                                 >  1:174020/1‑154 (MQ=255)                                                                                                                                                   tcatTAAGCAGGCAAAAATTGTAATTGTCATTTTATTCAAGGAGTTAATATGAAACTATATTTTAAACCGGGTGCATGCTCATTCGTACCGCATTTCGCATTGGAAAAAATCAATCAGCCTTATGAAGCCGAAGCAGTGACACAAGAGTATATGAAGAGCCCGGAATACTTAGAGCTCAATCCACAAGGTGCAGTTCCTCTACTCGTTGACGGt                                                                                                     <  1:534788/214‑1 (MQ=255)                                                                                                                                                   tcatTAAGCAGGCAAAAATTGTAATTGTCATTTTATTCAAGGAGTTAATATGAAACTATATTTTAAACCGGGTGCATGCTCATTCGTACCGCATTTCGCATTGGAAAAAATCAATCAGCCTTATGAAGCCGAAGCAGTGACACAAGAGTATATGAAGAGCCCGGAATACTTAGAGCTCAATCCACAAGGTGCAGTTCCTCTACTCGTTGACGGTAATCTTGTACTTTCCCAAAACTTGGCGATTTTAACtt                                                                >  1:669588/1‑251 (MQ=255)                                                                                                                                                               cAAAAATTGTAATTGTCATTTTATTCAAGGAGTTAATATGAAACTATATTTTAAACCGGGTGCATGCTCATTCGTACCGCATTTCGCATTGGAAAAAATCAATCAGCCTTATGAAGCCGAAGCAGTGACACAAGAGTATATGAAGAGCCCGGAATACtt                                                                                                                                                >  1:779675/1‑159 (MQ=255)                                                                                                                                                                      tgtaattgtCATTTTATTCAAGGAGTTAATATGAAACTATATTTTAAACCGGGTGCATGCTCATTCGTACCGCATTTCGCATTGGAAAAAATCAATCAGCCTTATGAAGCCGAAGCAGTGACACAAGAGTATATGAAGAGCCCGGAATACTTAGAGCTCAATCCACAAGGTGCAGTTCCTCTACTCGTTGACGGTAATCTTGTACTTTCCCAAAGCTTGGCGATTTTAACTTATTTAGACGATCTTTAccc                                             <  1:794778/251‑1 (MQ=255)                                                                                                                                                                      tgtaattgtCATTTTATTCAAGGAGTTAATATGAAACTATATTTTAAACCGGGTGCATGCTCATTCGTACCGCATTTCGCAGTGGAAAAAATCAATCAGCCTTATGAAGCCGAAGCAGTGACACAAGAGTATATGAAGAGCCCGGAATACTTAGAGCTCAATCCACAAGGTGCAGTTCCTCTACTCGTTGACGGTAATCTTGTACTTTCCCAAAACTTGGCGATTTTAACTTATTTAGACGATCTTTAccc                                             <  1:446511/251‑1 (MQ=255)                                                                                                                                                                          attgtCATTTTATTCAAGGAGTTAATATGAAACTATATTTTAAACCGGGTGCATGCTCATTCGTACCGCATTTCGCATTGGAAAAAATCAATCAGCCTTATGAAGCCGAAGCAGTGACACAAGAGTATATGAAGAGCCCGGAATACTTAGAGCTCAATCCACAAGGTGCAGTTCCTCTACTCGTTGACGGTAATCTTGTACTTTCCCAAAACTTGGCGATTTTAACTTATTTAGACGATCTTTACCCACAg                                         >  1:640317/1‑251 (MQ=255)                                                                                                                                                                                aTTTTATTCAAGGAGTTAATATGAAACTATATTTTAAACCGGGTGCATGCTCATTCGTACCGCATTTCGCATTGGAAAAAATCAATCAGCCTTATGAAGCCGAAGCAGTGACACAAGAGTATATGAAGAGCCCGGAATACTTAGAGCTCAATCCACAAGGTGCAGTTCCTCTACTCGTTGACGGTAATCTTGTACTTTCCCAAAACTTGGCGATTTTAACt                                                                 <  1:185831/221‑1 (MQ=255)                                                                                                                                                                                     aTTCAAGGAGTTAATATGAAACTATATTTTAAACCGGGTGCATGCTCATTCGTACCGCATTTCGCATTGGAAAAAATCAATCAGCCTTATGAAGCCGAAGCAGTGACACAAGAGTATATGAAGAGCCCGGAATACTTAGAGCTCAATCCACAAGGTGCAGTTCCTCTACTCGTTGACGGTAATCTTGTACTTTCCCAAAACTTGGCGATTTTAACTTATTTAGACGATCTTTACCCACAGGCAAAACTCtt                              >  1:34123/1‑251 (MQ=255)                                                                                                                                                                                            gAGTTAATATGAAACTATATTTTAAACCGGGTGCATGCTCATTCGTACCGCATTTCGCATTGGAAAAAATCAATCAGCCTTATGa                                                                                                                                                                                             <  1:859767/85‑1 (MQ=255)                                                                                                                                                                                              gTTAATATGAAACTATATTTTAAACCGGGTGCATGCTCATTCGTACCGCATTTCGCATTGGAAAAAATCAATCAGCCTTATGAAGCCGAAGCAGTGACACAAGAGTATATGAAGAGCCCGGAATACTTAGAGCTCAATCCACAAGGTGCAGTTCCTCTACTCGTTGACGGTAATCTTGTAc                                                                                           <  1:794809/181‑1 (MQ=255)                                                                                                                                                                                              gTTAATATGAAACTATATTTTAAACCGGGAGCATGATCATTCGTACCGCATTTCGCATTGGAAAAAATTAATCAGCCTTATGACGCCGACGCAGTGACACAAGAGTATATGAAGAGCCCGGAATACTTATAGCTCAATCCACAAGGTGCAGCTCCTCTACTCGTTGACGGAAATCTTGTACTTTCCCAAAACTTGGCGATTTTAACTTATTTAGACGATCTTTACCCACAGCCAAAACTCTTTGGCAGCGa                     >  1:359800/1‑251 (MQ=255)                                                                                                                                                                                                  atatGAAACTATATTTTAAACCGGGTGCATGCTCATTCGTACCGCATTTCGCATTGGAAAAAATCAATCAGCCTTATGAAGCCGAAGCAGTGACACAAGAGTATATGAAGAGCCCGGAATACTTAGAGCTCAATCCACAAGGTGCAGTTCCTCTACTCGTTGACGGTAATCTTGTACTTTCCCAAAACTTGGCGATTTTAACTTATTTAGACGATCTTTACCCACAGGCAAAACTCTTTGGCAGCGACTCa                 >  1:29605/1‑251 (MQ=255)                                                                                                                                                                                                         aCTATATTTTAAACCGGGTGCATGCTCATTCGTACCGCATTTCGCATTGGAAAAAATCAATCAGCCTTATGAAGCCGAAGCAGTGACACAAGAGTATATGAAGAGCCCGGAATACTTAGAGCTCAATCCACAAGGTGCAGTTCCTCTACTCGTTGACGGTAATCTTGTACTTTCCCAAAAc                                                                                <  1:819747/181‑1 (MQ=255)                                                                                                                                                                                                          cTATATTTTAAACCGGGTGCATGCTCATTCGTACCGCATTTCGCATTGGAAAAAATCAATCAGCCTTATGAAGCCGAAGCAGTGACACAAGAGTATATGAag                                                                                                                                                              >  1:167376/1‑102 (MQ=255)                                                                                                                                                                                                          cTATATTTTAAACCGGGTGCATGCTCATTCGTACCGCATTTCGCATTGGAAAAAATCAATCAGCCTTATGAAGCCGAAGCAGTGACACAAGAGTATATGAAGAGCCCGGAATACTTAGAGCTCAATCCACAAGGTGCAGTTCCTCTACTCGTTGACGGTAATCTTGTACTTTCCCAAAACTTGGCGATTTTAACTTATTTAGACGATCTTTACCCACAGGCAAAACTCTTTGGCAGCGACTCAGCCAAAgg         >  1:198087/1‑251 (MQ=255)                                                                                                                                                                                                            atatTTTAAACCGGGTGCATGCTCATTCGTACCGCATTTCGCATTGGAAAAAATCAATCAGCCTTATGAAGCCGAAGCAGTGACACAAGAGTATATGAAGAGCCCGGAATACTTAGAGCTCAATCCACAAg                                                                                                                               >  1:444199/1‑131 (MQ=255)                                                                                                                                                                                                            atatTTTAAACCGGGTGCATGCTCATTCGTACCGCATTTCGCATTGGAAAAAATCAATCAGCCTTATGAAGCCGAAGCAGTGACACAAGAGTATATGAAGAGCCCGGAATACTTAGAGCTCAATCCACAAGGTGCAGTTCCTCTACTCGTTGACGGTAATCTTGTACTTTCCCAAAAc                                                                                <  1:734135/178‑1 (MQ=255)                                                                                                                                                                                                              atTTTAAACCGGGTGCATGCTCATTCGTACCGCATTTCGCATTGGAAAAAATCAATCAGCCTTATGAAGCCGAAGCAGTGACACAAGAGTATATGAAGAGCCCGGAATACTTAGAGCTCAATCCACAAGGTGCAGTTCCTCTACTCGTTGACGGTAATCTTGTAc                                                                                           <  1:31610/165‑1 (MQ=255)                                                                                                                                                                                                              atTTTAAACCGGGTGCATGCTCATTCGTACCGCATTTCGCATTGGAAAAAATCAATCAGCCTTATGAAGCCGAAGCAGTGACACAAGAGTATATGAAGAGCCCGGAATACTTAGAGCTCAATCCACAAGGTGCAGTTCCTCTACTCGTTGACGGTAATCTTGTACTTTCCCAAAACTTGGCGATTTTAACTTATTTAGACGATCTTTAcc                                              >  1:370839/1‑210 (MQ=255)                                                                                                                                                                                                                 ttAAACCGGGTGCATGCTCATTCGTACCGCATTTCGCATTGGAAAAAATCAATCAGCCTTATGAAGCCGAAGCAGTGACACAAGAGTATATGAAGAGCCCGGAATACTTAGAGCTCAATCCACAAGGTGCAGTTCCTCTACTCGTTGACGGTAATCTTGTACTTTCCCAAAACTTGGCGATTTTAACTTATTTAGACGATCTTTACCCACAGGCAAAACTCTTTGGCAGCGACTCAGCCAAAGGCAAAGCt  <  1:118038/251‑1 (MQ=255)                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                       AAACAAGATAGAAAGAATTTTATTCACTACTGAGTGTAACTAAGCCGGCACGTTGTATATATCCATAAATGGAAATATCAATAGATTCAATCAGGTGGGCATATTACACAAATCTGCTACAATGTCCCTGATGGTTATTTATACCATCATTAAGCAGGCAAAAATTGTAATTGTCATTTTATTCAAGGAGTTAATATGAAACTATATTTTAAGCCGGGTGCATGCTCATTCGTACCGCATTTCGCATTGGAAAAAATCAATCAGCCTTATGAAGCCGAAGCAGTGACACAAGAGTATATGAAGAGCCCGGAATACTTAGAGCTCAATCCACAAGGTGCAGTTCCTCTACTCGTTGACGGTAATCTTGTACTTTCCCAAAACTTGGCGATTTTAACTTATTTAGACGATCTTTACCCACAGGCAAAACTCTTTGGCAGCGACTCAGCCAAAGGCAAAGCT  >  1/1730179‑1730637

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑