Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,709,434	G→A	intergenic (‑39486/‑10449)	unknown ← / → unknown	tRNA‑Arg‑CCG/tRNA‑Asn‑GTT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,709,434	0	G	A	100.0%	42.9 / NA	18	intergenic (‑39486/‑10449)	unknown/unknown	tRNA‑Arg‑CCG/tRNA‑Asn‑GTT
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (8/10);  total (8/10)

ACGGAAGCCTGCGCCATAGCAGCGACAATTTCCGGTTTATCCATCGGACCATCATCCACCGGCTGACAAGAGGCAATTAATCCGTTCTGAAGTTGGGCGAAAACGGCTTGATGAGATAATTTTTGAGATAGCTGTGACATAGAAACTCCTCTTTTTTGCGGCTGCATTTGGCAGTCATTCTAGTCAATGGAGACATTCTACTAAAATAAAATTTCATTTTAAAATATTTTTTGAAGTAATATTCCAACTATGTGATCTACTTCTCATTTTTGAAATAAAGCTTCATTCTCTATTTAAAAACGATTTTTTTACTTTAGAATGCAAACTGAGCTGTAATTCAAACATCCCATATTCATTATTTGTTACTACAGGAGTGTAGCCATGAAACTAGCCAAACTTTTCCTTGCCACTGCAATTGCACTAGGTGTGACCTCTGTTGCCAATGCCGCAGAATATGATTT  >  1/1709199‑1709659                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                  aCGGAAGCCTGCGCCATAGCAGCGACAATTTCCGGTTTATCCATCGGACCATCATCCACCGGCTGACAAGAGGCAATTAATCCGTTCTGAAGTTGGGCGAAAACGGCTTGATGAGATAATTTTTGAGATAGCTGTGACATAGAAACTCCTCTTTTTTGCGGCTGCATTTGGCAGTCATTCTAGTCAGTGGAGACATTCTACTAAAATAAAATTTCATTTTAAAATATTTTTTGAAATAATATTCCAACTAt                                                                                                                                                                                                                    >  1:209759/1‑251 (MQ=255)          tGCGCCATAGCAGCGACAATTTCCGGTTTATCCATCGGACCATCATCCACCGGCTGACAAGAGGCAATTAATCCGTTCTGAAGTTGGGCGAAAACGGCTTGATGAGATAATTTTTGAGATAGCTGTGACATAGAAACTCCTCTTTTTTGCGGCTGCATTTGGCAGTCATTCTAGTCAGTGGAGACATTCTACTAAAATAAAATTTCATTTTAAAATATTTTTTGAAATAATATTCCAACTATGTGATCTAc                                                                                                                                                                                                           <  1:387401/251‑1 (MQ=255)          tGCGCCATAGCAGCGACAATTTCCGGTTTATCCATCGGACCATCATCCACCGGCTGACAAGAGGCAATTAATCCGTTCTGAAGTTGGGCGAAAACGGCTTGATGAGATAATTTTTGAGATAGCTGTGACATAGAAACTCCTCTTTTTTGCGGCTGCATTTGGCAGTCATTCTAGTCAGTGGAGACATTCTACTAAAATAAAATTTCATTTTAAAATATTTTTTGAAATAATATTCCAACTATGTGATCTAc                                                                                                                                                                                                           <  1:45375/251‑1 (MQ=255)                                                       tcCACCGGCTGACAAGAGGCAATTAATCCGTTCTGAAGTTGGGCGAAAACGGCTTGATGAGATAATTTTTGAGATAGCTGTGACATAGAAACTCCTCTTTTTTGCGGCTGCATTTGGCAGTCATTCTAGTCAGTGGAGACATTCTACTAAAATAAAATTTCATTTTAAAATATTTTTTGAAATAATATTCCAACTATGTGATCTACTTCTCATTTTTGAAATAAAGc                                                                                                                                                                                      <  1:388346/227‑1 (MQ=255)                                                                          cAATTAATCCGTTCTGAAGTTGGGCGAAAACGGCTTGATGAGATAATTTTTGAGATAGCTGTGACATAGAAACTCCTCTTTTTTGCGGCTGCATTTGGCAGTCATTCTAGTCAGTGGAGACATTCTACTAAAATAAAATTTCATTTTAAAATATTTTTTGAAATAATATTCCAACTATGTGATCTACTTCTCATTTTTGAAATAAAGCTTCATTCTCTATTTAAAAACGATTTTTTTACTTTAGAATGCaa                                                                                                                                           >  1:794681/1‑251 (MQ=255)                                                                                    gTTCTGAAGTTGGGCGAAAACGGCTTGATGAGATAATTTTTGAGATAGCTGTGACATAGAAACTCCTCTTTTTTGCGGCTGCATTTGGCAGTCATTCTAGTCAGTGGAGACATTCTACTAAAATAAAATTTCATTTTAAAATATTTTTTGAAATAATATTCCAACTATGTGATCTAc                                                                                                                                                                                                           >  1:718156/1‑177 (MQ=255)                                                                                                     aaaCGGCTTGATGAGATAATTTTTGAGATAGCTGTGACATAGAAACTCCTCTTTTTTGCGGCTGCATTTGGCAGTCATTCTAGTCAGTGGAGACATTCTACTAAAATAAAATTTCATTTTAAAATATTTTTTGAAATAATATTCCAACTATGTGATCTACTTCTCATTTTTGAAATAAAGCTTCATTCTCTATTTAAAAACGATTTTTTTACTTTAGAATGCAAACTGAGCTGTAATTCAAACATCCCata                                                                                                                <  1:771915/251‑1 (MQ=255)                                                                                                         ggCTTGATGAGATAATTTTTGAGATAGCTGTGACATAGAAACTCCTCTTTTTTGCGGCTGCATTTGGCAGTCATTCTAGTCAGTGGAGACATTCTACTAAAATAAAATTTCATTTTAAAATATTTTTTGAAATAATATTCCAACTATGTGATCTACTTCTCATTTTTGAAATAAAGCTTCATtc                                                                                                                                                                               <  1:390569/184‑1 (MQ=255)                                                                                                                            tGAGATAGCTGTGACATAGAAACTCCTCTTTTTTGCGGCTGCATTTGGCAGTCATTCTAGTCAGTGGAGACATTCTACTAAAATAAAATTTCATTTTAAAATATTTTTTGTAATAATATTCCAACTATGTGATCTACTTCTCATTTTTGAAATAAAGCTTCATTCTCTATTTAAAAACGATTTTTTTACTTTAGAATGCAAACTGAGCTGTAATTCAAACATCCCATATTCATTATTTGTTACTACAGGAg                                                                                         <  1:237341/251‑1 (MQ=255)                                                                                                                            tGAGATAGCTGTGACATAGAAACTCCTCTTTTTTGCGGCTGCATTTGGCAGTCATTCTAGTCAGTGGAGACATTCTACTAAAATAAAATTTCATTTTAAAATATTTTTTGAAATAATATTCCAACTATGTGATCTACTTCTCATTTTTGAAATAAAGCTTCATTCTCTATTTAAAAACGATTTTTTTACTTTAGAATGCAAACTGAGCTGTAATTCAAACATCCCATATTCATTATTTGTTACTACAGGAg                                                                                         <  1:71736/251‑1 (MQ=255)                                                                                                                                          cATAGAAACTCCTCTTTTTTGCGGCTGCATTTGGCAGTCATTCTAGTCAGTGGAGACATTCTACTAAAATAAAATTTCATTTTAAAATATTTTTTGAAATAATATTCCAACTATGTGATCTACTTCTCATTTTTGAAATAAAGCTTCATtc                                                                                                                                                                               <  1:756924/151‑1 (MQ=255)                                                                                                                                                  ctcctcTTTTTTGCGGCTGCATTTGGCAGTCATTCTAGTCAGTGGAGACATTCTACTAAAATAAAATTTCATTTTAAAATATTTTTTGAAATAATATTCCAACTATGTGATCTACTTCTCATTTTTGAAATAAAGCTTCATTCTCTATTTAAAAACGATTTTTTTACTTTAGAATGCAAACTGAGCTGTAATTCAAACATCCCATAtt                                                                                                              >  1:56457/1‑208 (MQ=255)                                                                                                                                                                                 aTTCTAGTCAGTGGAGACATTCTACTAAAATAAAATTTCATTTTAAAATATTTTTTGAAATAATATTCCAACTATGTGATCTACTTCTCATTTTTGAAATAAAGCTTCATTCTCTATTTAAAAACGATTTTTTTACTTTAGAATGCAAACTGAGCTGTAATTCAAACATCCCATATTCATTATTTGTTACTACAGGAGTGTAGCCATGAAACTAGCCAAACTTTTCCTTGCCACTGCAATTGCACTAGgtg                                    >  1:636916/1‑251 (MQ=255)                                                                                                                                                                                      aGTCAGTGGAGACATTCTACTAAAATAAAATTTCATTTTAAAATATTTTTTGAAATAATATTCCAACTATGTGATCTACTTCTCATTTTTGAAATAAAGCTTCATTCTCTATTTAAAAACGATTTTTTTACTTTAGAATGCAAACTGAGCTGTAATTCAAACATCCCATATTCATTATTTGTTACTACAGGAg                                                                                         >  1:106523/1‑193 (MQ=255)                                                                                                                                                                                           gtGGAGACATTCTACTAAAATAAAATTTCATTTTAAAATATTTTTTGAAATAATATTCCAACTATGTGATCTACTTCTCATTTTTGAAATAAAGCTTCATTCTCTATTTAAAAACGATTTTTTTACTTTAGAATGCAAACTGAGCTGTAATTCAAACATCCCa                                                                                                                  >  1:196451/2‑163 (MQ=255)                                                                                                                                                                                           gtGGAGACATTCTACTAAAATAAAATTTCATTTTAAAATATTTTTTGAAATAATATTCCAACTATGTGATCTACTTCTCATTTTTGAAATAAAGCTTCATTCTCTATTTAAAAACGATTTTTTTACTTTAGAATGCAAACTGAGCTGTAATTCAAACATCCCATATTCATTATTTGTTACTACAgg                                                                                           >  1:92351/2‑186 (MQ=255)                                                                                                                                                                                              gagaCATTCTACTAAAATAAAATTTCATTTTAAAATATTTTTTGAAATAATATTCCAACTATGTGATCTACTTCTCATTTTTGAAATAAAGCTTCATTCTCTATTTAAAAACGATTTTTTTACTTTAGAATGCAAACTGAGCTGTAATTCAAACATCCCATATTCATTATTTGTTACTACAGGAGTGTAGCCATGAAACTAGCCAAACTTTTCCTTGCCACTGCAATTGCACTAGGTGTGACCTCTGTTg                        <  1:298774/250‑1 (MQ=255)                                                                                                                                                                                                                   atTTCATTTTAAAATATTTTTTGAAATAATATTCCAACTATGTGATCTACTTCTCATTTTTGAAATAAAGCTTCATTCTCTATTTAAAAACGATTTTTTTACTTTAGAATGCAAACTGAGCTGTAATTCAAACATCCCATATTCATTATTTGTTACTACAGGAGTGTAGCCATGAAACTAGCCAAACTTTTCCTTGCCACTGCAATTGCACTAGGTGTGACCTCTGTTGCCAATGCCGCAGAATATGAttt  <  1:779217/251‑1 (MQ=255)                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                  ACGGAAGCCTGCGCCATAGCAGCGACAATTTCCGGTTTATCCATCGGACCATCATCCACCGGCTGACAAGAGGCAATTAATCCGTTCTGAAGTTGGGCGAAAACGGCTTGATGAGATAATTTTTGAGATAGCTGTGACATAGAAACTCCTCTTTTTTGCGGCTGCATTTGGCAGTCATTCTAGTCAATGGAGACATTCTACTAAAATAAAATTTCATTTTAAAATATTTTTTGAAGTAATATTCCAACTATGTGATCTACTTCTCATTTTTGAAATAAAGCTTCATTCTCTATTTAAAAACGATTTTTTTACTTTAGAATGCAAACTGAGCTGTAATTCAAACATCCCATATTCATTATTTGTTACTACAGGAGTGTAGCCATGAAACTAGCCAAACTTTTCCTTGCCACTGCAATTGCACTAGGTGTGACCTCTGTTGCCAATGCCGCAGAATATGATTT  >  1/1709199‑1709659

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑