Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,690,270	A→G	intergenic (‑20322/‑29613)	unknown ← / → unknown	tRNA‑Arg‑CCG/tRNA‑Asn‑GTT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,690,270	0	A	G	100.0%	33.3 / NA	13	intergenic (‑20322/‑29613)	unknown/unknown	tRNA‑Arg‑CCG/tRNA‑Asn‑GTT
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (9/4);  total (9/4)

TTTTAATAGAATCACACTTTATTGAATTAAGCAATAAACCACTAAATACCTTTAAGATAACTTCTTTTGTAGATTCCGATATGGATACATTATTAATATAAACTTTTAATTTTAAATTACCAACAATTTTTTCATCTCCATATATATAATATGTAATCATATCTGAAAATGAATTTTCTTAATCTATTAGTAATGGCCTACTATACGCCCACCATCTATTATCATAGCCAGGGTGATAGTCAGTCATTATAATTTTGTCATTTTTAAGAAAATTTCTAGCCAAATATGGAGACCAGCCTTTCTTTGAAGGAACAAAAGGAGGATTAGATTCTACACCTTTTAATTTCTTTATGTTACATACACTTCTTTCTAAATCTTCTTTATCTCTTATTTGGTAGATT  >  1/1690090‑1690490                                                                                                                                                                                     |                                                                                                                                                                                                                             ttttAATAGAATCACACTTTATTGAATTAAGCAATAAACCACTAAATACCTTTAAGATAACTTCTTTTGTAGATTCCGATATGGATACATTATTAATATAAACTTTTAATTTTAAATTACCAACAATTTTTTCATCTCCATATATATAATATGTAATCATATCTGAAAATGAATTTTCTTGATCTATTAGTAATGGCCTACTATACGCCCACCATCTATTATCATAGCCAGGGTGATAGTCAGTCATtata                                                                                                                                                        >  1:279661/1‑251 (MQ=255)                   ttATTGAATTAAGCAATAAACCACTAAATACCTTTAAGATAACTTCTTTTGTAGATTCCGATATGGATACATTATTAATATAAACTTTTAATTTTAAATTACCAACAATTTTTTCATCTCCATATATATAATATGTAATCATATCTGAAAATGAATTTTCTTGATCTATTa                                                                                                                                                                                                                      >  1:654399/1‑171 (MQ=255)                                                                                   ggATACATTATTAATATAAACTTTTAATTTTAAATTACCAACAATTTTTTCATCTCCATATATATAATATGTAATCATATCTGAAAATGAATTTTCTTGATCTATTAGTAATGGCCTACtat                                                                                                                                                                                                       >  1:209049/1‑122 (MQ=255)                                                                                       aCATTATTAATATAAACTTTTAATTTTAAATTACCAACAATTTTTTCATCTCCATATATATAATATGTAATCATATCTGAAAATGAATTTTCTTGATCTATTAGTAATGGCCTACTATACGCCCACCATCTATTATCAt                                                                                                                                                                                  >  1:121143/1‑139 (MQ=255)                                                                                        cATTATTAATATAAACTTTTAATTTTAAATTACCAACAATTTTTTCATCTCCATATATATAATATGTAATCATATCTGAAAATGAATTTTCTTGATCTATTAGTAATGGCCTACTATACGCCCACCATCTATTATCATAGCCAGGGTGATAGTCAGTCATTATAATTTTGTCATTTTTAAGAAAATTTCTAGCCAAATATGGAGACCAGCCTTTCTTTGAAGGAACAAAAGGAGGATTAGATTCTacac                                                                   <  1:606323/249‑1 (MQ=255)                                                                                                                                            atatatatAATATGTAATCATATCTGAAAATGAATTTTCTTGATCTATTAGTAATGGCCTACTATACGCCCACCATCTATTATCATAGCCAGGGTGATAGTCAGTCATTATAATTTTGTCATTTTTAAGAAAATTTCTAGCCAAATATGGAGAc                                                                                                              >  1:478575/1‑154 (MQ=255)                                                                                                                                               tatataATATGTAATCATATCTGAAAATGAATTTTCTTGATCTATTAGTAATGGCCTACTATACGCCCACCATCTATTATCATAGCCAGGGTGATAGTCAGTCATTATAATTTTGTCATTTTTAAGAAAATTTCTAGCCAAATATGGAGAc                                                                                                              <  1:381103/151‑1 (MQ=255)                                                                                                                                                atataATATGTAATCATATCTGAAAATGAATTTTCTTGATCTATTAGTAATGGCCTACTATACGCCCACCATCTATTATCATAGCCAGGGTGATAGTCAGTCATTATAATTTTGTCATTTTTAAGAAAATTTCTAGCCAAATATGGAGACCAGCCTTTCTTTGAAGGAACAAAAGGAGGATTAGATTCTacac                                                                   >  1:530078/1‑193 (MQ=255)                                                                                                                                                     atatGTAATCATATCTGAAAATGAATTTTCTTGATCTATTAGTAATGGCCTACTATACGCCCACCATCTATTATCATAGCCAGGGTGATAGTCAGTCATTATAATTTTGTCATTTTTAAGAAAATTTCTAGCCAAATATGgag                                                                                                                >  1:816409/1‑143 (MQ=255)                                                                                                                                                       atGTAATCATATCTGAAAATGAATTTTCTTGATCTATTAGTAATGGCCTACTATACGCCCACCATCTATTATCATAGCCAGGGTGATAGTCAGTCATTATAATTTTGTCATTTTTAAGAAAATTTCTAGCCAAATATGGAGACCAGCCTTTCTTTGAAGGAACAAAAGGAGGATTAGATTCTACACCTTTTAATTTCTTTATGTTACATACACTTCTTTCTAAATCTTCTTTATCTCTTATTTGGTAGAtt  >  1:718000/1‑251 (MQ=255)                                                                                                                                                            aTCATATCTGAAAATGAATTTTCTTGATCTATTAGTAATGGCCTACTATACGCCCACCATCTATTATCATAGCCAGGGTGATAGTCAGTCATTATAATTTTGTCATTTTTAAGAAAATTTCTAGCCAAATATGGAGAc                                                                                                              >  1:410472/1‑138 (MQ=255)                                                                                                                                                            aTCATATCTGAAAATGAATTTTCTTGATCTATTAGTAATGGCCTACTATACGCCCACCATCTATTATCATAGCCAGGGTGATAGTCAGTCATTATAATTTTGTCATTTTTAAGAAAATTTCTAGCCAAATATGGAGACCAGCCTTTCTTTGAAGGAACAAAagg                                                                                    <  1:249531/164‑1 (MQ=255)                                                                                                                                                                 atCTGAAAATGAATTTTCTTGATCTATTAGTAATGGCCTACTATACGCCCACCATCTATTATCATAGCCAGGGTGATAGTCAGTCATTATAATTTTGTCATTTTTAAGAAAATTTCTAGCCAAATATGGAGACCAGCCTTTCTTTGAAGGAACAAAAGGAGGATTAGATTCTACACCTTTTAAt                                                           <  1:626064/184‑1 (MQ=255)                                                                                                                                                                                     |                                                                                                                                                                                                                             TTTTAATAGAATCACACTTTATTGAATTAAGCAATAAACCACTAAATACCTTTAAGATAACTTCTTTTGTAGATTCCGATATGGATACATTATTAATATAAACTTTTAATTTTAAATTACCAACAATTTTTTCATCTCCATATATATAATATGTAATCATATCTGAAAATGAATTTTCTTAATCTATTAGTAATGGCCTACTATACGCCCACCATCTATTATCATAGCCAGGGTGATAGTCAGTCATTATAATTTTGTCATTTTTAAGAAAATTTCTAGCCAAATATGGAGACCAGCCTTTCTTTGAAGGAACAAAAGGAGGATTAGATTCTACACCTTTTAATTTCTTTATGTTACATACACTTCTTTCTAAATCTTCTTTATCTCTTATTTGGTAGATT  >  1/1690090‑1690490

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑