Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,689,840	T→C	intergenic (‑19892/‑30043)	unknown ← / → unknown	tRNA‑Arg‑CCG/tRNA‑Asn‑GTT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,689,840	0	T	C	100.0%	251.9 / NA	86	intergenic (‑19892/‑30043)	unknown/unknown	tRNA‑Arg‑CCG/tRNA‑Asn‑GTT
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (37/49);  total (37/49)

TGCATTAATGGTTCTTAATCGGTCGCTAATCGAATTATATTTTGCATGCTTTATCGGTTTATCAAATAATCCGGTAAAGGTCGGAGAACTGTAAAATTGCTCTAATGTTCTTAAGCTGTTATGTCGTACATTTAATGATGAAAATAGCAGTAAATAAAACATCACTTCACCGCTTAATTTTTTCACTTGACGGTCAACTTGATATTCCAATGCAAACATTCTTAACTGTTCTTTCGGAATATAGCTTAAGATTTCTCTGAGTTTCATCGCTCACCTCACTCTCTGTTAATAAGGTGATTTTACCTTTTTCTCAGGTTTCGAACAGCTATGGTCGGGAGACGCTCGCCATCGTGGGGGGATAATATTATTCTAATTTAATTGATATATTCAATTCCAAAGTATAGCCTCTATTATTTGTAAAATTCTCCTTAGTAAAACTTATAATA  >  1/1689595‑1690040                                                                                                                                                                                                                                                      |                                                                                                                                                                                                         tGCATTAATGGTTCTTAATCGGTCGCTAATCGAATTATATTTTGCATGCTTTATCGGTTTATCAAATAATCCGGTAAAGGTCGGAGAACTGTAAAATTGCTCTAATGTTCTTAAGCTGTTATGTCGTACATTTAATGATGAAAATAGCAGTAAATAAAACATCACTTCACCGCTTAATTTTTTCACTTGACGGTCAACTTGATATTCCAATGCAAACATTCTTAACTGTTCTTTCGGAATATAGCCTAAGa                                                                                                                                                                                                     <  1:41366/251‑1 (MQ=255)  gCATTAATGGTTCTTAATCGGTCGCTAATCGAATTATATTTTGCATGCTTTATCGGTTTATCAAATAATCCGGTAAAGGTCGGAGAACTGTAAAATTGCTCTAATGTTCTTAAGCTGTTATGTCGTACATTTAATGATGAAAATAGCAGTAAATAAAACATCACTTCACCGCTTAATTTTTTCACTTGACGGTCAACTTGATATTCCAATGCAAACATTCTTAACTGTTCTTTCGGAATATAGCCTAAGAt                                                                                                                                                                                                    >  1:392427/1‑251 (MQ=255)  gCATTAATGGTTCTTAATCGGTCGCTAATCGAATTATATTTTGCATGCTTTATCGGTTTATCAAATAATCCGGTAAAGGTCGGAGAACTGTAAAATTGCTCTAATGTTCTTAAGCTGTTATGTCGTACATTTAATGATGAAAATAGCAGTAAATAAAACATCACTTCACCGCTTAATTTTTTCACTTGACGGTCAACTTGATATTCCAATGCAAACATTCTTAACTGTTCTTTCGGAATATAGCCTAAGAt                                                                                                                                                                                                    >  1:875345/1‑251 (MQ=255)  gCATTAATGGTTCTTAATCGGTCGCTAATCGAATTATATTTTGCATGCTTTATCGGTTTATCAAATAATCCGGTAAAGGTCGGAGAACTGTAAAATTGCTCTAATGTTCTTAAGCTGTTATGTCGTACATTTAATGATGAAAATAGCAGTAAATAAAACATCACTTCACCGCTTAATTTTTTCACTTGACGGTCAACTTGATATTCCAATGCAAACATTCTTAACTGTTCTTTCGGAATATAGCCTAAGAt                                                                                                                                                                                                    >  1:771210/1‑251 (MQ=255)        aTGGTTCTTAATCGGTCGCTAATCGAATTATATTTTGCATGCTTTATCGGTTTATCAAATAATCCGGTAAAGGTCGGAGAACTGTAAAATTGCTCTAATGTTCTTAAGCTGTTATGTCGTACATTTAATGATGAAAATAGCAGTAAATAAAACATCACTTCACCGCTTAATTTTTTCACTTGACGGTCAACTTGATATTCCAATGCAAACATTCTTAACTGTTCTTTCGGAATATAGCCTAAGATTTctct                                                                                                                                                                                              <  1:636712/251‑1 (MQ=255)           gTTCTTAATCGGTCGCTAATCGAATTATATTTTGCATGCTTTATCGGTTTATCAAATAATCCGGTAAAGGTCGGAGAACTGTAAAATTGCTCTAATGTTCTTAAGCTGTTATGTCGTACATTTAATGATGAAAATAGCAGTAAATAAAACATCACTTCACCGCTTAATTTTTTCACTTGACGGTCAACTTGATATTCCAATGCAAACATTCTTAACTGTTCTTTCGGAATATAGCCTAAGAt                                                                                                                                                                                                    >  1:122037/1‑242 (MQ=255)           gTTCTTAATCGGTCGCTAATCGAATTATATTTTGCATGCTTTATCGGTTTATCAAATAATCCGGTAAAGGTCGGAGAACTGTAAAATTGCTCTAATGTTCTTAAGCTGTTATGTCGTACATTTAATGATGAAAATAGCAGTAAATAAAACATCACTTCACCGCTTAATTTTTTCACTTGACGGTCAACTTGATATTCCAATGCAAACATTCTTAACTGTTCTTTCGGAATATAGCCTAAGATTTCTCTGAg                                                                                                                                                                                           >  1:397592/1‑251 (MQ=255)               ttAATCGGTCGCTAATCGAATTATATTTTGCATGCTTTATCGGTTTATCAAATAATCCGGTAAAGGTCGGAGAACTGTAAAATTGCTCTAATGTTCTTAAGCTGTTATGTCGTACATTTAATGATGAAAATAGCAGTAAATAAAACATCACTTCACCGCTTAATTTTTTCACTTGACGGTCAACTTGATATTCCAATGCAAACATTCTTAACTGTTCTTTCGGAATATAGCCTAAGATTTCTCTGAGttt                                                                                                                                                                                        >  1:454174/1‑250 (MQ=255)                    cGGTCGCTAATCGAATTATATTTTGCATGCTTTATCGGTTTATCAAATAATCCGGTAAAGGTCGGAGAACTGTAAAATTGCTCTAATGTTCTTAAGCTGTTATGTCGTACATTTAATGATGAAAATAGCAGTAAATAAAACATCACTTCACCGCTTAATTTTTTCGCTTGACGGTCAACTTGATATTCCAATGCAAACATTCTTAACTGTTCTTTCGGAATATAGCCTAAGATTTCTCTGAGTTTCATCGc                                                                                                                                                                                  <  1:103410/251‑1 (MQ=255)                       tCGCTAATCGAATTATATTTTGCATGCTTTATCGGTTTATCAAATAATCCGGTAAAGGTCGGAGAACTGTAAAATTGCTCTAATGTTCTTAAGCTGTTATGTCGTACATTTAATGATGAAAATAGCAGTAAATAAAACATCACTTCACCGCTTAATTTTTTCACTTGACGGTCAACTTGATATTCCAATGCAAACATTCTTAACTGTTCTTTCGGAATATAGCCTAAGATTTCTCTGAGTTTCATCGctc                                                                                                                                                                                <  1:463183/250‑1 (MQ=255)                           tAATCGAATTATATTTTGCATGCTTTATCGGTTTATCAAATAATCCGGTAAAGGTCGGAGAACTGTAAAATTGCTCTAATGTTCTTAAGCTGTTATGTCGTACATTTAATGATGAAAATAGCAGTAAATAAAACATCACTTCACCGCTTAATTTTTTCACTTGACGGTCAACTTGATATTCCAATGCAAACATTCTTAACTGTTCTTTCGGAATATAGCCTAAGATTTCTCTGAGTTTCATCGctcacctc                                                                                                                                                                           <  1:661033/251‑1 (MQ=255)                                 aaTTATATTTTGCATGCTTTATCGGTTTATCAAATAATCCGGTAAAGGTCGGAGAACTGTAAAATTGCTCTAATGTTCTTAAGCTGTTATGTCGTACATTTAATGATGAAAATAGCAGTAAATAAAACATCACTTCACCGCTTAATTTTTTCACTTGACGGTCAACTTGATATTCCAATGCAAACATTCTTAACTGTTCTTTCGGAATATAGCCTAAGATTTCTCTGAGTTTCATCGCTCACCTCActct                                                                                                                                                                      <  1:461323/250‑1 (MQ=255)                                 aaTTATATTTTGCATGCTTTATCGGTTTATCAAATAATCCGGTAAAGGTCGGAGAACTGTAAAATTGCTCTAATGTTCTTAAGCTGTTATGTCGTACATTTAATGATGAAAATAGCAGTAAATAAAACATCACTTCACCGCTTAATTTTTTCACTTGACGGTCAACTTGATATTCCAATGCAAACATTCTTAACTGTTCTTTCGGAATATAGCCTAAGATTTCTCTGAGTTTCATCGCTCACCTCActct                                                                                                                                                                      >  1:270973/1‑250 (MQ=255)                                   ttATATTTTGCATGCTTTATCGGTTTATCAAATAATCCGGTAAAGGTCGGAGAACTGTAAAATTGCTCTAATGTTCTTAAGCTGTTATGTCGTACATTTAATGATGAAAATAGCAGTAAATAAAACATCACTTCACCGCTTAATTTTTTCACTTGACGGTCAACTTGATATTCCAATGCAAACATTCTTAACTGTTCTTTCGGAATATAGCCTAAGATTTCTCTGAGTTTCATCGCTCACCTCActct                                                                                                                                                                      >  1:612973/1‑248 (MQ=255)                                    tataTTTTGCATGCTTTATCGGTTTATCAAATAATCCGGTAAAGGTCGGAGAACTGTAAAATTGCTCTAATGTTCTTAAGCTGTTATGTCGTACATTTAATGATGAAAATAGCAGTAAATAAAACATCACTTCACCGCTTAATTTTTTCACTTGACGGTCAACTTGATATTCCAATGCAAACATTCTTAACTGTTCTTTCGGAATATAGCCTAAGATTTCTCTGAGTTTCATCGCTCACCTCACTCTCTGt                                                                                                                                                                  <  1:483144/251‑1 (MQ=255)                                     atatTTTGCATGCTTTATCGGTTTATCAAATAATCCGGTAAAGGTCGGAGAACTGTAAAATTGCTCTAATGTTCTTAAGCTGTTATGTCGTACATTTAATGATGAAAATAGCAGTAAATAAAACATCACTTCACCGCTTAATTTTTTCACTTGACGGTCAACTTGATATTCCAATGCAAACATTCTTAACTGTTCTTTCGGAATATAGCCTAAGATTTCTCTGAGTTTCATCGCTCACCTCACTCTCTGtt                                                                                                                                                                 >  1:691305/1‑251 (MQ=255)                                        ttttGCATGCTTTATCGGTTTATCAAATAATCCGGTAAAGGTCGGAGAACTGTAAAATTGCTCTAATGTTCTTAAGCTGTTATGTCGTACATTTAATGATGAAAATAGCAGTAAATAAAACATCACTTCACCGCTTAATTTTTTCACTTGACGGTCAACTTGATATTCCAATGCAAACATTCTTAACTGTTCTTTCGGAATATAGCCTAAGATTTCTCTGAGTTTCATCGCTCACCTCACTCTCTGTtaat                                                                                                                                                              <  1:773516/251‑1 (MQ=255)                                         tttGCATGCTTTATCGGTTTATCAAATAATCCGGTAAAGGTCGGAGAACTGTAAAATTGCTCTAATGTTCTTAAGCTGTTATGTCGTACATTTAATGATGAAAATAGCAGTAAATAAAACATCACTTCACCGCTTAATTTTTTCACTTGACGGTCAACTTGATATTCCAATGCAAACATTCTTAACTGTTCTTTCGGAATATAGCCTAAGATTTCTCTGAGTTTCATCGCTCACCTCActctc                                                                                                                                                                     >  1:317832/1‑243 (MQ=255)                                          gtGCATGCTTTATCGGGTTAATAAATAATCCGGTAAAGGTCGGAGAACTGTAAAATTGCTCTAATGTTCTTAAGCTGTTATGTCGTACATTTAATGATGAAAATAGCAGTAAATAAAACATCACTTCACCGCTTAATTTTTTCACTTGACGGTCAACTTGATATTCCAATGCAAACATTCTTAACTGTTCTTTCGGAATATAGCCTAAGATTTCTCTGAGTTTCATCGCTCACCTCACTCTCTGTtaat                                                                                                                                                              <  1:305104/248‑1 (MQ=255)                                                 cTTTATCGGTTTATCAAATAATCCGGTAAAGGTCGGAGAACTGTAAAATTGCTCTAATGTTCTTAAGCTGTTATGTCGTACATTTAATGATGAAAATAGCAGTAAATAAAACATCACTTCACCGCTTAATTTTTTCACTTGACGGTCAACTTGATATTCCAATGCAAACATTCTTAACTGTTCTTTCGGAATATAGCCTAAGATTtctc                                                                                                                                                                                               >  1:698003/1‑209 (MQ=255)                                                 cTTTATCGGTTTATCAAATAATCCGGTAAAGGTCGGAGAACTGTAAAATTGCTCTAATGTTCTTAAGCTGTTATGTCGTACATTTAATGATGAAAATAGCAGTAAATAAAACATCACTTCACCGCTTAATTTTTTCACTTGACGGTCAACTTGATATTCCAATGCAAACATTCTTAACTGTTCTTTCGGAATATAGCCTAAGATTTCTCTGAGTTTCATCGCTCACCTCACTCTCTGTtaata                                                                                                                                                             <  1:86967/243‑1 (MQ=255)                                                 cTTTATCGGTTTATCAAATAATCCGGTAAAGGTCGGAGAACTGTAAAATTGCTCTAATGTTCTTAAGCTGTTATGTCGTACATTTAATGATGAAAATAGCAGTAAATAAAACATCACTTCACCGCTTAATTTTTTCACTTGACGGTCAACTTGATATTCCAATGCAAACATTCTTAACTGTTCTTTCGGAATATAGCCTAAGATTTCTCTGAGTTTCATCGCTCACCTCACTCTCTGTTAATAAGGTGAtt                                                                                                                                                     >  1:887318/1‑251 (MQ=255)                                                 cTTTATCGGTTTATCAAATAATCCGGTAAAGGTCGGAGAACTGTAAAATTGCTCTAATGTTCTTAAGCTGTTATGTCGTACATTTAATGATGAAAATAGCAGTAAATAAAACATCACTTCACCGCTTAATTTTTTCACTTGACGGTCAACTTGATATTCCAATGCAAACATTCTTAACTGTTCTTTCGGAATATAGCCTAAGATTTCTCTGAGTTTCATCGCTCACCTCACTCTCTGTTAATAAGGTGAtt                                                                                                                                                     >  1:70764/1‑251 (MQ=255)                                                               cAAATAATCCGGTAAAGGTCGGAGAACTGTAAAATTGCTCTAATGTTCTTAAGCTGTTATGTCGTACATTTAATGATGAAAATAGCAGTAAATAAAACATCACTTCACCGCTTAATTTTTTCACTTGACGGTCAACTTGATATTCCAATGCAAACATTCTTAACTGTTCTTTCGGAATATAGCCTAAGAt                                                                                                                                                                                                    <  1:874901/190‑1 (MQ=255)                                                                 aataatCCGGTAAAGGTCGGAGAACTGTAAAATTGCTCTAATGTTCTTAAGCTGTTATGTCGTACATTTAATGATGAAAATAGCAGTAAATAAAACATCACTTCACCGCTTAATTTTTTCACTTGACGGTCAACTTGATATTCCAATGCAAACATTCTTAACTGTTCTTTCGGAATATAGCCTAAGATTTCTCTGAGTTTCATCGCTCACCTCACTCTCTGTTAATAAGGTGATTTTACCTTTTTCTCAgg                                                                                                                                     <  1:9174/251‑1 (MQ=255)                                                                     atCCGGTAAAGGTCGGAGAACTGTAAAATTGCTCTAATGTTCTTAAGCTGTTATGTCGTACATTTAATGATGAAAATAGCAGTAAATAAAACATCACTTCACCGCTTAATTTTTTCACTTGACGGTCAACTTGATATTCCAATGCAAACATTCTTAACTGTTCTTTCGGAATATAGCCTAAGATTTCTCTGAGTTTCATCGCTCACCTCACTCTCTGTTAATAAGGTg                                                                                                                                                        <  1:791549/228‑1 (MQ=255)                                                                               ggTCGGAGAACTGTAAAATTGCTCTAATGTTCTTAAGCTGTTATGTCGTACATTTAATGATGAAAATAGCAGTAAATAAAACATCACTTCACCGCTTAATTTTTTCACTTGACGGTCAACTTGATATTCCAATGCAAACATTCTTAACTGTTCTTTCGGAATATAGCCTAAGATTTCTCTGAGTTTCATCGCTCACCTCACTCTCTGTTAATAAGGTGATTTTACCTTTTTCTCAGGTTTCGAACAg                                                                                                                           >  1:796598/1‑247 (MQ=255)                                                                                 tCGGAGAACTGTAAAATTGCTCTAATGTTCTTAAGCTGTTATGTCGTACATTTAATGATGAAAATAGCAGTAAATAAAACATCACTTCACCGCTTAATTTTTTCACTTGACGGTCAACTTGATATTCCAATGCAAACATTCTTAACTGTTCTTTCGGAATATAGCCTAAGATTTCTCTGAGTTTCATCGCTCACCTCACTCTCTGTTAATAAGGTGATTTTACCTTTTTCTCAGGTTTCGAACAGCTATgg                                                                                                                     <  1:379480/251‑1 (MQ=255)                                                                                  cGGAGAACTGTAAAATTGCTCTAATGTTCTTAAGCTGTTATGTCGTACATTTAATGATGAAAATAGCAGTAAATAAAACATCACTTCACCGCTTAATTTTTTCACTTGACGGTCAACTTGATATTCCAATGCAAACATTCTTAACTGTTCTTTCGGAATATAGCCTAAGATTTCTCTGAGTTTCATCGCTCACCTCACTCTCTGTTAATAAGGTGATTTTACCTTTTTCTCAGGTTTCGAACAGCTATGGt                                                                                                                    <  1:94782/251‑1 (MQ=255)                                                                                    gagaACTGTAAAATTGCTCTAATGTTCTTAAGCTGTTATGTCGTACATTTAATGATGAAAATAGCAGTAAATAAAACATCACTTCACCGCTTAATTTTTTCACTTGACGGTCAACTTGATATTCCAATGCAAACATTCTTAACTGTTCTTTCGGAATATAGCCTAAGATTTCTCTGAGTTTCATCGCTCACCTCACTCTCTGTTAATAAGGTGATTTTACCTTTTTCTCAGGTTTCGAACAGCTATGGTc                                                                                                                   <  1:683293/250‑1 (MQ=255)                                                                                        aCTGTAAAATTGCTCTAATGTTCTTAAGCTGTTATGTCGTACATTTAATGATGAAAATAGCAGTAAATAAAACATCACTTCACCGCTTAATTTTTTCACTTGACGGTCAACTTGATATTCCAATGCAAACATTCTTAACTGTTCTTTCGGAATATAGCCTAAGATTTCTCTGAGTTTCATCGCTCACCTCACTCTCTGTTAATAAGGTGATTTTACCTTTTTCTCAGGTTTCGAACAGCTATGGTCGGgag                                                                                                              <  1:636680/251‑1 (MQ=255)                                                                                         cTGTAAAATTGCTCTAATGTTCTTAAGCTGTTATGTCGTACATTTAATGATGAAAATAGCAGTAAATAAAACATCACTTCACCGCTTAATTTTTTCACTTGACGGTCAACTTGATATTCCAATGCAAACATTCTTAACTGTTCTTTCGGAATATAGCCTAAGATTTCTCTGAGTTTCATCGCTCACCTCACTCTCTGTtaata                                                                                                                                                             <  1:356233/203‑1 (MQ=255)                                                                                         cTGTAAAATTGCTCTAATGTTCTTAAGCTGTTATGTCGTACATTTAATGATGAAAATAGCAGTAAATAAAACATCACTTCACCGCTTAATTTTTTCACTTGACGGTCAACTTGATATTCCAATGCAAACATTCTTAACTGTTCTTTCGGAATATAGCCTAAGATTTCTCTGAGTTTCATCGCTCACCTCACTCTCTGTtaata                                                                                                                                                             >  1:327073/1‑203 (MQ=255)                                                                                         cTGTAAAATTGCTCTAATGTTCTTAAGCTGTTATGTCGTACATTTAATGATGAAAATAGCAGTAAATAAAACATCACTTCACCGCTTAATTTTTTCACTTGACGGTCAACTTGATATTCCAATGCAAACATTCTTAACTGTTCTTTCGGAATATAGCCTAAGATTTCTCTGAGTTTCATCGCTCACCTCACTCTCTGTTAATAAGGTGATTTTACCTTTTTCTCAGGTTTCGAACAGCTATGGTCGGgaga                                                                                                             <  1:120592/251‑1 (MQ=255)                                                                                         cTGTAAAATTGCTCTAATGTTCTTAAGCTGTTATGTCGTACATTTAATGATGAAAATAGCAGTAAATAAAACATCACTTCACCGCTTAATTTTTTCACTTGACGGTCAACTTGATATTCCAATGCAAACATTCTTAACTGTTCTTTCGGAATATAGCCTAAGATTTCTCTGAGTTTCATCGCTCACCTCACTCTCTGTTAATAAGGTGATTTTACCTTTTTCTCAGGTTTCGAACAGCTATGGTCGGgaga                                                                                                             <  1:292869/251‑1 (MQ=255)                                                                                         cTGTAAAATTGCTCTAATGTTCTTAAGCTGTTATGTCGTACATTTAATGATGAAAATAGCAGTAAATAAAACATCACTTCACCGCTTAATTTTTTCACTTGACGGTCAACTTGATATTCCAATGCAAACATTCTTAACTGTTCTTTCGGAATATAGCCTAAGATTTCTCTGAGTTTCATCGCTCACCTCACTCTCTGTTAATAAGGTGATTTTACCTTTTTCTCAGGTTTCGAACAGCTATGGTCGGgaga                                                                                                             <  1:359352/251‑1 (MQ=255)                                                                                           gTAAAATTGCTCTAATGTTCTTAAGCTGTTATGTCGTACATTTAATGATGAAAATAGCAGTAAATAAAACATCACTTCACCGCTTAATTTTTTCACTTGACGGTCAACTTGATATTCCAATGCAAACATTCTTAACTGTTCTTTCGGAATATAGCCTAAGATTTCTCTGAGTTTCATCGCTCACCTCACTCTCTGTTAATAAGGTGATTTTACCTTTTTCTCAGGTTTCGAACAGCTATGGTCGGGAGACg                                                                                                           >  1:273014/1‑251 (MQ=255)                                                                                           gTAAAATTGCTCTAATGTTCTTAAGCTGTTATGTCGTACATTTAATGATGAAAATAGCAGTAAATAAAACATCACTTCACCGCTTAATTTTTTCACTTGACGGTCAACTTGATATTCCAATGCAAACATTCTTAACTGTTCTTTCGGAATATAGCCTAAGATTTCTCTGAGTTTCATCGCTCACCTCACTCTCTGTTAATAAGGTGATTTTACCTTTTTCTCAGGTTTCGAACAGCTATGGTCGGGAGACg                                                                                                           >  1:854388/1‑251 (MQ=255)                                                                                                   gCTCTAATGTTCTTAAGCTGTTATGTCGTACATTTAATGATGAAAATAGCAGTAAATAAAACATCACTTCACCGCTTAATTTTTTCACTTGACGGTCAACTTGATATTCCAATGCAAACATTCTTAACTGTTCTTTCGGAATATAGCCTAAGATTTCTCTGAGTTTCATCGCTCACCTCACTCTCTGTTAATAAGGTGATTTTACCTTTTTCTCAGGTTTCGAACAGCTATGGTc                                                                                                                   <  1:785741/235‑1 (MQ=255)                                                                                                        aaTGTTCTTAAGCTGTTATGTCGTACATTTAATGATGAAAATAGCAGTAAATAAAACATCACTTCACCGCTTAATTTTTTCACTTGACGGTCAACTTGATATTCCAATGCAAACATTCTTAACTGTTCTTTCGGAATATAGCCTAAGATTTCTCTGAGTTTCATCGCTCACCTCACTCTCTGtt                                                                                                                                                                 <  1:161703/184‑1 (MQ=255)                                                                                                          tGTTCTTAAGCTGTTATGTCGTACATTTAATGATGAAAATAGCAGTAAATAAAACATCACTTCACCGCTTAATTTTTTCACTTGACGGTCAACTTGATATTCCAATGCAAACATTCTTAACTGTTCTTTCGGAATATAGCCTAAGATTTCTCTGAGTTTCATCGCTCACCTCACTCTCTGTTAATAAGGTGATTTTACCTTTTTCTCAGGTTTCGAACAGCTATGGTCGGGAGACGCTCGCCATCGTgggg                                                                                            <  1:415516/251‑1 (MQ=255)                                                                                                           gTTCTTAAGCTGTTATGTCGTACATTTAATGATGAAAATAGCAGTAAATAAAACATCACTTCACCGCTTAATTTTTTCACTTGACGGTCAACTTGATATTCCAATGCAAACATTCTTAACTGTTCTTTCGGAATATAGCCTAAGATTTCTCTGAGTTTCATCGCTCACCTCACTCTCTGTTAATAAGGTGATTTTAcc                                                                                                                                                >  1:620203/1‑198 (MQ=255)                                                                                                            ttCTTAAGCTGTTATGTCGTACATTTAATGATGAAAATAGCAGTAAATAAAACATCACTTCACCGCTTAATTTTTTCACTTGACGGTCAACTTGATATTCCAATGCAAACATTCTTAACTGTTCTTTCGGAATATAGCCTAAGATTTCTCTGAGTTTCATCGCTCACCTCACTCTCTGTTAATAAGGTGATTTTACCTTTTTCTCAGGTTTCGAACAGCTATGGTCGGGAGACGCTCGCCATCGTgggggg                                                                                          >  1:472364/1‑251 (MQ=255)                                                                                                                   gCTGTTATGTCGTACATTTAATGATGAAAATAGCAGTAAATAAAACATCACTTCACCGCTTAATTTTTTCACTTGACGGTCAACTTGATATTCCAATGCAAACATTCTTAACTGTTCTTTCGGAATATAGCCTAAGATTTCTCTGAGTTTCATCGCTCACCTCACTCTCTGTTAATAAGGTGATTTTACCTTTTTCTCAGGTTTCGAACAGCTGTGGtttcctaacgtgtgcctgaaatatc                                                                                            >  1:311509/1‑218 (MQ=255)                                                                                                                   gCTGTTATGTCGTACATTTAATGATGAAAATAGCAGTAAATAAAACATCACTTCACCGCTTAATTTTTTCACTTGACGGTCAACTTGATATTCCAATGCAAACATTCTTAACTGTTCTTTCGGAATATAGCCTAAGATTTCTCTGAGTTTCATCGCTCACCTCACTCTCTGTTAATAAGGTGATTTTACCTTTTTCTCAGGTTTCGAACAGCTATGGTCGGGAGACGCTCGCCATCGTGGGGGGATAatat                                                                                   >  1:220290/1‑251 (MQ=255)                                                                                                                    cTGTTATGTCGTACATTTAATGATGAAAATAGCAGTAAATAAAACATCACTTCACCGCTTAATTTTTTCACTTGACGGTCAACTTGATATTCCAATGCAAACATTCTTAACTGTTCTTTCGGAATATAGCCTAAGATTTCTCTGAGTTTCATCg                                                                                                                                                                                   <  1:818447/154‑1 (MQ=255)                                                                                                                    cTGTTATGTCGTACATTTAATGATGAAAATAGCAGTAAATAAAACATCACTTCACCGCTTAATTTTTTCACTTGACGGTCAACTTGATATTCCAATGCAAACATTCTTAACTGTTCTTTCGGAATATAGCCTAAGATTTCTCTGAGTTTCATCGCTCACCTCACTCTCTGtt                                                                                                                                                                 <  1:224215/172‑1 (MQ=255)                                                                                                                    cTGTTATGTCGTACATTTAATGATGAAAATAGCAGTAAATAAAACATCACTTCACCGCTTAATTTTTTCACTTGACGGTCAACTTGATATTCCAATGCAAACATTCTTAACTGTTCTTTCGGAATATAGCCTAAGATTTCTCTGAGTTTCATCGCTCACCTCACTCTCTGTtaata                                                                                                                                                             >  1:291568/1‑176 (MQ=255)                                                                                                                    cTGTTATGTCGTACATTTAATGATGAAAATAGCAGTAAATAAAACATCACTTCACCGCTTAATTTTTTCACTTGACGGTCAACTTGATATTCCAATGCAAACATTCTTAACTGTTCTTTCGGAATATAGCCTAAGATTTCTCTGAGTTTCATCGCTCACCTCACTCTCTGTTAATAAGGTGATTTTACCTTTTTCTCAGGTTTCGAACAGCTATGGTCGGGAGACGCTCGCCATCGTGGGGGGATAatat                                                                                   <  1:321399/250‑1 (MQ=255)                                                                                                                    cTGTTATGTCGTACATTTAATGATGAAAATAGCAGTAAATAAAACATCACTTCACCGCTTAATTTTTTCACTTGACGGTCAACTTGATATTCCAATGCAAACATTCTTAACTGTTCTTTCGGAATATAGCCTAAGATTTCTCTGAGTTTCATCGCTCACCTCACTCTCTGTTAATAAGGTGATTTTACCTTTTTCTCAGGTTTCGAACAGCTATGGTCGGGAGACGCTCGCCATCGTGGGGGGATAatat                                                                                   <  1:475602/250‑1 (MQ=255)                                                                                                                      gTTATGTCGTACATTTAATGATGAAAATAGCAGTAAATAAAACATCACTTCACCGCTTAATTTTTTCACTTGACGGTCAACTTGATATTCCAATGCAAACATTCTTAACTGTTCTTTCGGAATATAGCCTAAGATTTCTCTGAGTTTCATCGCTCACCTCACTCTCTGTTAATAAGGTGATTTTACCTTTTTCTCAGGTTTCGAACAGCTATGGTCGGGAGACGCTCGCCATCGTGGGGGGATAatat                                                                                   >  1:508963/1‑248 (MQ=255)                                                                                                                        tATGTCGTACATTTAATGATGAAAATAGCAGTAAATAAAACATCACTTCACCGCTTAATTTTTTCACTTGACGGTCAACTTGATATTCCAATGCAAACATTCTTAACTGTTCTTTCGGAATATAGCCTAAGATTTCTCTGAGTTTCATCGCTCACCTCACTCTCTGTTAATAAGGTGATTTTACCTTTTTCTCAGGTTTCGAACAGCTATGGTCGGGAGACGCTCGCCATCGTGGGGGGATAATATTATTc                                                                              <  1:398931/251‑1 (MQ=255)                                                                                                                        tATGTCGTACATTTAATGATGAAAATAGCAGTAAATAAAACATCACTTCACCGCTTAATTTTTTCACTTGACGGTCAACTTGATATTCCAATGCAAACATTCTTAACTGTTCTTTCGGAATATAGCCTAAGATTTCTCTGAGTTTCATCGCTCACCTCACTCTCTGTTAATAAGGTGATTTTACCTTTTTCTCAGGTTTCGAACAGCTATGGTCGGGAGACGCTCGCCATCGTGGGGGGATAATATTATTc                                                                              <  1:426036/251‑1 (MQ=255)                                                                                                                           gTCGTACATTTAATGATGAAAATAGCAGTAAATAAAACATCACTTCACCGCTTAATTTTTTCACTTGACGGTCAACTTGATATTCCAATGCAAACATTCTTAACTGTTCTTTCGGAATATAGCCTAAGATTTCTCTGAGTTTCATCGCTCACCTCACTCTCTGTTAATAAGGTGATTTTACCTTTTTCTCAGGTTTCGAAc                                                                                                                             <  1:403995/201‑1 (MQ=255)                                                                                                                           gTCGTACATTTAATGATGAAAATAGCAGTAAATAAAACATCACTTCACCGCTTAATTTTTTCACTTGACGGTCAACTTGATATTCCAATGCAAACATTCTTAACTGTTCTTTCGGAATATAGCCTAAGATTTCTCTGAGTTTCATCGCTCACCTCACTCTCTGTTAATAAGGTGATTTTACCTTTTTCTCAGGTTTCGAACAGCTATGGTCGGGAGACGCTCGCCATCGTGGGGGGATAATATTATTc                                                                              >  1:263493/1‑248 (MQ=255)                                                                                                                           gTCGTACATTTAATGATGAAAATAGCAGTAAATAAAACATCACTTCACCGCTTAATTTTTGCACTTGACGGTCAACTTGATATTCCAATGCAAACATTCTTAACTGTTCTTTCGGAATATAGCCTAAGATTTCTCTGAGTTTCATCGCTCACCTCACTCTCTGTTAATAAGGTGATTTTACCTTTTTCTCAGGTTTCGAACAGCTATGGTCGGGAGACGCTCGCCATCGt                                                                                                <  1:429306/230‑1 (MQ=255)                                                                                                                                 cATTTAATGATGAAAATAGCAGTAAATAAAACATCACTTCACCGCTTAATTTTTTCACTTGACGGTCAACTTGATATTCCAATGCAAACATTCTTAACTGTTCTTTCGGAATATAGCCTAAGATTTCTCTGAGTTTCATCGCTCACCTCActc                                                                                                                                                                       <  1:256563/153‑1 (MQ=255)                                                                                                                                 cATTTAATGATGAAAATAGCAGTAAATAAAACATCACTTCACCGCTTAATTTTTTCACTTGACGGTCAACTTGATATTCCAATGCAAACATTCTTAACTGTTCTTTCGGAATATAGCCTAAGATTTCTCTGAGTTTCATCGCTCACCTCACTCTCTGTTAATAAgg                                                                                                                                                          >  1:533988/1‑166 (MQ=255)                                                                                                                                 cATTTAATGATGAAAATAGCAGTAAATAAAACATCACTTCACCGCTTAATTTTTTCACTTGACGGTCAACTTGATATTCCAATGCAAACATTCTTAACTGTTCTTTCGGAATATAGCCTAAGATTTCTCTGAGTTTCATCGCTCACCTCACTCTCTGCTAATAAGGTGATTTTACCTTTTTCTCAGGTTTCGAACAGCTATGGTCGGGAGACGCTCGCCATCGTgg                                                                                              <  1:523847/226‑1 (MQ=255)                                                                                                                                           tgaAAATAGCAGTAAATAAAACATCACTTCACCGCTTAATTTTTTCACTTGACGGTCAACTTGATATTCCAATGCAAACATTCTTAACTGTTCTTTCGGAATATAGCCTAAGATTTCTCTGAGTTTCATCGCTCACCTCACTCTCTGTTAATAAGGTGATTTTACCTTTTTCTCAGGTTTCGAACAGCTATGGTCGGGAGACGCTCGCCATCGTGGGGGGATAATATTATTCTAATTTAATTGATATATTc                                                           <  1:712635/251‑1 (MQ=255)                                                                                                                                            gaAAATAGCAGTAAATAAAACATCACTTCACCGCTTAATTTTTTCACTTGACGGTCAACTTGATATTCCAATGCAAACATTCTTAACTGTTCTTTCGGAATATAGCCTAAGATTTCTCTGAGTTTCATCGCTCACCTCACTCTCTGTTAATAAGGTGATTTTACCTTTTTCTCAGGTTTCGAACAGCTATGGTCGGgaga                                                                                                             <  1:463044/200‑1 (MQ=255)                                                                                                                                            gaAAATAGCAGTAAATAAAACATCACTTCACCGCTTAATTTTTTCACTTGACGGTCAACTTGATATTCCAATGCAAACATTCTTAACTGTTCTTTCGGAATATAGCCTAAGATTTCTCTGAGTTTCATCGCTCACCTCACTCTCTGTTAATAAGGTGATTTTACCTTTTTCTCAGGTTTCGAACAGCTATGGTCGGGAGACGCTCGCCATCGTGGGGGGATAATATTATTc                                                                              >  1:173274/1‑231 (MQ=255)                                                                                                                                                      gTAAATAAAACATCACTTCACCGCTTAATTTTTTCACTTGACGGTCAACTTGATATTCCAATGCAAACATTCTTAACTGTTCTTTCGGAATATAGCCTAAGATTTCTCTGAGTTTCATCGCTCACCTCACTCTCTGTtaata                                                                                                                                                             <  1:793503/142‑1 (MQ=255)                                                                                                                                                        aaataaaACATCACTTCACCGCTTAATTTTTTCACTTGACGGTCAACTTGATATTCCAATGCAAACATTCTTAACTGTTCTTTCGGAATATAGCCTAAGATTTCTCTGAGTTTCATCGCTCACCTCACTCTCTGTtaata                                                                                                                                                             >  1:26621/1‑140 (MQ=255)                                                                                                                                                        aaataaaACATCACTTCACCGCTTAATTTTTTCACTTGACGGTCAACTTGATATTCCAATGCAAACATTCTTAACTGTTCTTTCGGAATATAGCCTAAGATTTCTCTGAGTTTCATCGCTCACCTCACTCTCTGTTAATAAGGTGATTTTACCt                                                                                                                                               <  1:255196/154‑1 (MQ=255)                                                                                                                                                        aaataaaACATCACTTCACCGCTTAATTTTTTCACTTGACGGTCAACTTGATATTCCAATGCAAACATTCTTAACTGTTCTTTCGGAATATAGCCTAAGATTTCTCTGAGTTTCATCGCTCACCTCACTCTCTGTTAATAAGGTGATTTTACCTTTTTCTCAGGTTTCGAACAGCTATGGTCGGGAGACGCTCGCCATCGTGGGGGGATAATATTATTc                                                                              >  1:530891/1‑219 (MQ=255)                                                                                                                                                        aaataaaACATCACTTCACCGCTTAATTTTTTCACTTGACGGTCAACTTGATATTCCAATGCAAACATTCTTAACTGTTCTTTCGGAATATAGCCTAAGATTTCTCTGAGTTTCATCGCTCACCTCACTCTCTGTTAATAAGGTGATTTTACCTTTTTCTCAGGTTTCGAACAGCTATGGTCGGGAGACGCTCGCCATCGTGGGGGGATAATATTATTCTAATTTAATTGATATATTc                                                           <  1:311729/238‑1 (MQ=255)                                                                                                                                                          ataaaACATCACTTCACCGCTTAATTTTTTCACTTGACGGTCAACTTGATATTCCAATGCAAACATTCTTAACTGTTCTTTCGGAATATAGCCTAAGATTTCTCTGAGTTTCATCGCTCACCTCACTCTCTGTTAATAAGGTGATTTTACCTTTTTCTCAGGTTTCGAACAGCTa                                                                                                                        >  1:792424/1‑175 (MQ=255)                                                                                                                                                             aaaCATCACTTCACCGCTTAATTTTTTCACTTGACGGTCAACTTGATATTCCAATGCAAACATTCTTAACTGTTCTTTCGGAATATAGCCTAAGATTTCTCTGAGTTTCATCGCTCACCTCACTCTCTGTTAATAAGGTGATTTTACCTTTTTCTCAGGTTTCGAACAGCTATGGTCGGGAGACGCTCGCCATCGTGGGGGGATAATATTATTCTAATTTAATTGATATATTCAATTCCAAAGTATAGCct                                         <  1:690560/251‑1 (MQ=255)                                                                                                                                                             aaaCATCACTTCACCGCTTAATTTTTTCACTTGACGGTCAACTTGATATTCCAATGCAAACATTCTTAACTGTTCTTTCGGAATATAGCCTAAGATTTCTCTGAGTTTCATCGCTCACCTCACTCTCTGTTAACAAGGTGATTTTACCTTTTTCTCAGGTTTCGAACAGCTATGGTCGGGAGACGCTCGCCATCGTGGGGGGATAATATTATTCTAATTTAATTGATATATTCAATTCCAAAGTATAGCct                                         <  1:223762/251‑1 (MQ=255)                                                                                                                                                                  tCACTTCACCGCTTAATTTTTTCACTTGACGGTCAACTTGATATTCCAATGCAAACATTCTTAACTGTTCTTTCGGAATATAGCCTAAGATTTCTCTGAGTTTCATCGCTCACCTCACTCTCTGTTAATAAGGTGATTTTACCTTTTTCTCAGGTTTCGAACAGCTATGGTc                                                                                                                   >  1:836525/1‑172 (MQ=255)                                                                                                                                                                   cACTTCACCGCTTAATTTTTTCACTTGACGGTCAACTTGATATTCCAATGCAAACATTCTTAACTGTTCTTTCGGAATATAGCCTAAGATTTCTCTGAGTTTCATCGCTCACCTCACTCTCTGTTAATAAGGTGATTTTACCTTTTTCTCAGGTTTCGAACAGCTATGGTCGGGAGACGCTCGCCATCGTGGGGGGATAATATTATTCTAATTTAATTGATATATTCAATTCCAAAGTATAGCCTCtatta                                   <  1:376455/251‑1 (MQ=255)                                                                                                                                                                    aCTTCACCGCTTAATTTTTTCACTTGACGGTCAACTTGATATTCCAATGCAAACATTCTTAACTGTTCTTTCGGAATATAGCCTAAGATTTCTCTGAGTTTCATCGCTCACCTCACTCTCTGTTAATAAGGTGATTTTACCTTTTTCTCAGGTTTCGAACAGCTATGGTCGGGAGACGCTCGCCATCGTGGGGGGATAATATTATTCTAATTTAATTGATATATTCAATTCCAAAGTATAGCCTCtattat                                  <  1:463899/251‑1 (MQ=255)                                                                                                                                                                            gCTTAATTTTTTCACTTGACGGTCAACTTGATATTCCAATGCAAACATTCTTAACTGTTCTTTCGGAATATAGCCTAAGATTTCTCTGAGTTTCATCGCTCACCTCACTCTCTGTTAATAAGGTGATTTTACCTTTTTCTCAGGTTTCGAACAGCTATGGTCGGgag                                                                                                              <  1:412498/167‑1 (MQ=255)                                                                                                                                                                                                    aaCTTGATATTCCAATGCAAACATTCTTAACTGTTCTTTCGGAATATAGCCTAAGATTTCTCTGAGTTTCATCGCTCACCTCACTCTCTGTTAATAAGGTGATTTTACCTTTTTCTCAGGTTTCGAACAGCTATGGTCGGGAGACGCTCGCCATCGTGGGGGGATAATATTATTCTAATTTAATTGATATATTCAATTCCAAAGTATAGCCTCTATTATTTGTAAAATTCTCCTTAGTAAAACTtataata  <  1:848504/251‑1 (MQ=255)                                                                                                                                                                                                     aCTTGATATTCCAATGCAAACATTCTTAACTGTTCTTTCGGAATATAGCCTAAGATTTCTCTGAGTTTCATCGctcacc                                                                                                                                                                             >  1:409676/1‑79 (MQ=255)                                                                                                                                                                                                        tGATATTCCAATGCAAACATTCTTAACTGTTCTTTCGGAATATAGCCTAAGATTTCTCTGAGTTTCATCGCTCACCTCACTCTCTGTTAATAAGGTGATTTTACCTTTTTCTCAGGTTTCGAACAGCTATGGTCGGGAGACGCTCGCCATCGTGGGGGGATAatat                                                                                   <  1:317036/166‑1 (MQ=255)                                                                                                                                                                                                        tGATATTCCAATGCAAACATTCTTAACTGTTCTTTCGGAATATAGCCTAAGATTTCTCTGAGTTTCATCGCTCACCTCACTCTCTGTTAATAAGGTGATTTTACCTTTTTCTCAGGTTTCGAACAGCTATGGTCGGGAGACGCTCGCCATCGTGGGGGGATAATATTATTCTAATTTAATTGATATATTCAATTCCAAAGTATAGcc                                          <  1:304780/207‑1 (MQ=255)                                                                                                                                                                                                                         cATTCTTAACTGTTCTTTCGGAATATAGCCTAAGATTTCTCTGAGTTTCATCGCTCACCTCACTCTCTGTTAATAAGGTGATTTTACCTTTTTCTCAGGTTTCGAACAGCTATGGTCGGGAGACGCTCGCCATCGTGGGGGGATAATATTATTCtaatt                                                                         >  1:290822/1‑159 (MQ=255)                                                                                                                                                                                                                               tAACTGTTCTTTCGGAATATAGCCTAAGATTTCTCTGAGTTTCATCGCTCACCTCACTCTCTGTTAATAAGGTGATTTTACCTTTTTCTCAGGTTTCGAACAGCTATGGTCGGGAGACGCTCGCCATCGTGGGGGGATAATATTATTc                                                                              >  1:198811/1‑148 (MQ=255)                                                                                                                                                                                                                                    gTTCTTTCGGAATATAGCCTAAGATTTCTCTGAGTTTCATCGCTCACCTCACTCTCTGTTAATAAGGTGATTTTACCTTTTTCTCAGGTTTCGAACAGCTATGGTCGGgaga                                                                                                             <  1:36925/112‑1 (MQ=255)                                                                                                                                                                                                                                    gTTCTTTCGGAATATAGCCTAAGATTTCTCTGAGTTTCATCGCTCACCTCACTCTCTGTTAATAAGGTGATTTTACCTTTTTCTCAGGTTTCGAACAGCTATGGTCGGGAGACGCTCGCCATCGTGGGGGGATAATATTATTCTAATTTAATTGATATATTCAATTCCAAAGTATAGcc                                          <  1:372063/179‑1 (MQ=255)                                                                                                                                                                                                                                             gAATATAGCCTAAGATTTCTCTGAGTTTCATCGCTCACCTCACTCTCTGTtaata                                                                                                                                                             >  1:128815/1‑55 (MQ=255)                                                                                                                                                                                                                                             gAATATAGCCTAAGATTTCTCTGAGTTTCATCGCTCACCTCACTCTCTGTTAATAAGGTGATTTTACCTTTTTCTCAGGTTTCGAACAGCTATGGTCGGGAGACGCTCGCCATCGTGGGGGGATAATATTATTCTAATTTAATTGATATATTCAATTCCAAAGTATAGCCTCtat                                     >  1:158951/1‑175 (MQ=255)                                                                                                                                                                                                                                               atatAGCCTAAGATTTCTCTGAGTTTCATCGCTCACCTCACTCTCTGTTAATAAGGTGATTTTACCTTTTTCTCAGGTTTCGAACAGCTATGGTCGGGAGACGCTCGCCATCGTGGGGGGATAATATTATTc                                                                              >  1:771873/1‑132 (MQ=255)                                                                                                                                                                                                                                                  taGCCTAAGATTTCTCTGAGTTTCATCGCTCACCTCACTCTCTGTTAATAAGGAGATTTTACCTTTTTCTCAGGTTTCGAACAGCTATGGTCGGgag                                                                                                              >  1:398575/1‑97 (MQ=255)                                                                                                                                                                                                                                                      |                                                                                                                                                                                                         TGCATTAATGGTTCTTAATCGGTCGCTAATCGAATTATATTTTGCATGCTTTATCGGTTTATCAAATAATCCGGTAAAGGTCGGAGAACTGTAAAATTGCTCTAATGTTCTTAAGCTGTTATGTCGTACATTTAATGATGAAAATAGCAGTAAATAAAACATCACTTCACCGCTTAATTTTTTCACTTGACGGTCAACTTGATATTCCAATGCAAACATTCTTAACTGTTCTTTCGGAATATAGCTTAAGATTTCTCTGAGTTTCATCGCTCACCTCACTCTCTGTTAATAAGGTGATTTTACCTTTTTCTCAGGTTTCGAACAGCTATGGTCGGGAGACGCTCGCCATCGTGGGGGGATAATATTATTCTAATTTAATTGATATATTCAATTCCAAAGTATAGCCTCTATTATTTGTAAAATTCTCCTTAGTAAAACTTATAATA  >  1/1689595‑1690040

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑