Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,687,617	A→G	intergenic (‑17669/‑32266)	unknown ← / → unknown	tRNA‑Arg‑CCG/tRNA‑Asn‑GTT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,687,617	0	A	G	100.0%	190.0 / NA	69	intergenic (‑17669/‑32266)	unknown/unknown	tRNA‑Arg‑CCG/tRNA‑Asn‑GTT
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (34/35);  total (34/35)

TCAACACCTTCACCCAAGACCAAATCATCAACGCTCTGCAAGACCGCAAGAGCGGTAAAATCGAAGGCGCCGGCAACCGCGCCAAATCCACCCTCAGCGAACAGGATATGCAAGATGTGGCAGGCTATGTGGAAACGTTGAAGAAATAATGGAAAA‑GTGCGGTCGGTTTTGG‑AAA‑TGTTTTTTGAGAACAAAATGAAGGTTCAAACATCCAATGCTTAA‑ACCTGATCAGAACAAGCTATGAATGCTTGCGCTATGCCAAAACAGCAGGGG‑CAGGTCCTGTGCCTGCCCGAATTTTT‑CGATGAATCCAACGGGCGGGCACAGGA‑C‑CCGCCCCTACGCGAAAACACAAAAAAACACCTTTGATAGCGCACGCTTCATAGCGCGTGCTTTTAACTTATTGATATTACAGGCACGCGTTAGGAAACGCGCACCAGCAAAATGTAACGTTTTTCAAAATTCAACTGTCATAAGGAAACTCTATG  >  1/1687380‑1687858                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                      tcaACACCTTCACCCAAGACCAAATCATCAACGCTCTGCAAGACCGCAAGAGCGGTAAAATCGAAGGCGCCGGCAACCGCGCCAAATCCACCCTCAGCGAACAGGATATGCAAGATGTGGCAGGCTATGTGGAAACGTTGAAGAAATAATGGAAAA‑GTGCGGTCGGTTTTGG‑AAA‑TGTTTTTTGAGAACAAAATGAAGGTTCAAACATCCAATGCTTAA‑ACCTGATCAGAACAAGCTGTGAATGCTTgcgc                                                                                                                                                                                                                                          <  1:268610/251‑1 (MQ=255) tcaACACCTTCACCCAAGACCAAATCATCAACGCTCTGCAAGACCGCAAGAGCGGTAAAATCGAAGGCGCCGGCAACCGCGCCAAATCCACCCTCAGCGAACAGGATATGCAAGATGTGGCAGGCTATGTGGAAACGTTGAAGAAATAATGGAAAA‑GTGCGGTCGGTTTTGG‑AAA‑TGTTTTTTGAGAACAAAATGAAGGTTCAAACATCCAATGCTTAA‑ACCTGATCAGAACAAGCTGTGAATGCTTgcgc                                                                                                                                                                                                                                          <  1:506349/251‑1 (MQ=255) tcaACACCTTCACCCAAGACCAAATCATCAACGCTCTGCAAGACCGCAAGAGCGGTAAAATCGAAGGCGCCGGCAACCGCGCCAAATCCACCCTCAGCGAACAGGATATGCAAGATGTGGCAGGCTATGTGGAAACGTTGAAGAAATAATGGAAAA‑GTGCGGTCGGTTTTGG‑AAA‑TGTTTTTTGAGAACAAAATGAAGGTTCAAACATCCAATGCTTAA‑ACCTGATCAGAACAAGCTGTGAATGCTTgcgc                                                                                                                                                                                                                                          <  1:265137/251‑1 (MQ=255)       cctacACCCAAGACCAAATCATCAACGCTCTGCAAGACCGCAAGAGCGGTAAAATCGAAGGCGCCGGCAACCGCGCCAAATCCACCCTCAGCGAACAGGATATGCAAGATGTGGCAGGCTATGTGGAAACGTTGAAGAAATAATGGAAAA‑GTGCGGTCGGTTTTGG‑AAA‑TGTTTTTTGAGAACAAAATGAAGGTTCAAACATCCAATGCTTAA‑ACCTGATCAGAACAAGCTGTGAATGCTTGCGCTATGc                                                                                                                                                                                                                                     >  1:449188/5‑250 (MQ=255)       ccTTCACCCAAGACCAAATCATCAACGCTCTGCAAGACCGCAAGAGCGGTAAAATCGAAGGCGCCGGCAACCGCGCCAAATCCACCCTCAGCGAACAGGATATGCAAGATGTGGCAGGCTATGTGGAAACGTTGAAGAAATAATGGAAAA‑GTGCGGTCGGTTTTGG‑AAA‑TGTTTTTTGAGAACAAAATGAAGGTTCAAACATCCAATGCTTAA‑ACCTGATCAGAACAAGCTGTGAATGCTTGCGCTATGc                                                                                                                                                                                                                                     >  1:449211/1‑250 (MQ=255)             cccAAGACCAAATCATCAACGCTCTGCAAGACCGCAAGAGCGGTAAAATCGAAGGCGCCGGCAACAGCGCCAAATCCACCCTCAGCGAACAGGATATGCAAGATGTGGCAGGCTATGTGGAAACGTTGAAGAAATAATGGAAAA‑GTGCGGTCGGTTTTGG‑AAA‑TGTTTTTTGAGAACAAAATGAAGGTTCAAACATCCAATGCTTAA‑ACCTGATCAGAACAAGCTGTGAATGCTTGCGCTATGCCAAAAca                                                                                                                                                                                                                              >  1:540341/1‑251 (MQ=255)                 aGACCAAATCATCAACGCTCTGCAAGACCGCAAGAGCGGTAAAATCGAAGGCGCCGGCAACCGCGCCAAATCCACCCTCAGCGAACAGGATATGCAAGATGTGGCAGGCTATGTGGAAACGTTGAAGAAATAATGGAAAA‑GTGCGGTCGGTTTTGG‑AAA‑TGTTTTTTGAGAACAAAATGAAGGTTCAAACATCCAATGCTTAA‑ACCTGATCAGAACAAGCTGTGAATGCTTGCGCTATGCCAAAAcagcag                                                                                                                                                                                                                          >  1:567929/1‑251 (MQ=255)                 aGACCAAATCATCAACGCTCTGCAAGACCGCAAGAGCGGTAAAATCGAAGGCGCCGGCAACCGCGCCAAATCCACCCTCAGCGAACAGGATATGCAAGATGTGGCAGGCTATGTGGAAACGTTGAAGAAATAATGGAAAA‑GTGCGGTCGGTTTTGG‑AAA‑TGTTTTTTGAGAACAAAATGAAGGTTCAAACATCCAATGCTTAA‑ACCTGATCAGAACAAGCTGTGAATGCTTGCGCTATGCCAAAAcagcag                                                                                                                                                                                                                          >  1:231267/1‑251 (MQ=255)                    ccAAATCATCAACGCTCTGCAAGACCGCAAGAGCGGTAAAATCGAAGGCGCCGGCAACCGCGCCAAATCCACCCTCAGCGAACAGGATATGCAAGATGTGGCAGGCTATGTGGAAACGTTGAAGAAATAATGGAAAA‑GTGCGGTCGGTTTTGG‑AAA‑TGTTTTTTGAGAACAAAATGAAGGTTCAAACATCCAATGCTTAA‑ACCTGATCAGAACAAGCTGTGAATGCTTGCGCTATGCCAAAACAGCAgggg                                                                                                                                                                                                                       <  1:358706/251‑1 (MQ=255)                          catcaACGCTCTGCAAGACCGCAAGAGCGGTAAAATCGAAGGCGCCGGCAACCGCGCCAAATCCACCCTCAGCGAACAGGATATGCAAGATGTGGCAGGCTATGTGGAAACGTTGAAGAAATAATGGAAAA‑GTGCGGTCGGTTTTGG‑AAA‑TGTTTTTTGAGAACAAAATGAAGGTTCAAACATCCAATGCTTAA‑ACCTGATCAGAACAAGCTGTGAATGCTTGCGCTATGCCAAAACAGCAGGGG‑CAGGt                                                                                                                                                                                                                 >  1:376788/1‑250 (MQ=255)                                  ctctGCAAGACCGCAAGAGCGGTAAAATCGAAGGCGCCGGCAACCGCGCCAAATCCACCCTCAGCGAACAGGATATGCAAGATGTGGCAGGCTATGTGGAAACGTTGAAGAAATAATGGAAAA‑GTGCGGTCGGTTTTGG‑AAA‑TGTTTTTTGAGAACAAAATGAAGGTTCAAACATCCAATGCTTAA‑ACCTGATCAGAACAAGCTGTGAATGCTTGCGCTATGCCAAAACAGCAGGGG‑CAGGTCCTGtgc                                                                                                                                                                                                          >  1:880061/1‑249 (MQ=255)                                  ctctGCAAGACCGCAAGAGCGGTAAAATCGAAGGCGCCGGCAACCGCGCCAAATCCACCCTCAGCGAACAGGATATGCAAGATGTGGCAGGCTATGTGGAAACGTTGAAGAAATAATGGAAAA‑GTGCGGTCGGTTTTGG‑AAA‑TGTTTTTTGAGAACAAAATGAAGGTTCAAACATCCAATGCTTAA‑ACCTGATCAGAACAAGCTGTGAATGCTTGCGCTATGCCAAAACAGCAGGGG‑CAGGTCCTGtgc                                                                                                                                                                                                          <  1:875183/249‑1 (MQ=255)                                    ctGCAAGACCGCAAGAGCGGTAAAATCGAAGGCGCCGGCAACCGCGCCAAATCCACCCTCAGCGAACAGGATATGCAAGATGTGGCAGGCTATGTGGAAACGTTGAAGAAATAATGGAAAA‑GTGCGGTCGGTTTTGG‑AAA‑TGTTTTTTGAGAACAAAATGAAGGTTCAAACATCCAATGCTTAA‑ACCTGATCAGAACAAGCTGTGAATGCTTGCGCTATGCCAAAACAGCAGGGG‑CAGGTCCTGtgcctg                                                                                                                                                                                                       >  1:238327/1‑250 (MQ=255)                                     tGCAAGACCGCAAGAGCGGTAAAATCGAAGGCGCCGGCAACCGCGCCAAATCCACCCTCAGCGAACAGGATATGCAAGATGTGGCAGGCTATGTGGAAACGTTGAAGAAATAATGGAAAA‑GTGCGGTCGGTTTTGG‑AAA‑TGTTTTTTGAGAACAAAATGAAGGTTCAAACATCCAATGCTTAA‑ACCTGATCAGAACAAGCTGTGAATGCTTGCGCTATGCCAAAACAGCAGGGG‑CAGGTCCTGtgcctgcc                                                                                                                                                                                                     <  1:466226/251‑1 (MQ=255)                                                       gTAAAATCGAAGGCGCCGGCAACCGCGCCAAATCCACCCTCAGCGAACAGGATATGCAAGATGTGGCAGGCTATGTGGAAACGTTGAAGAAATAATGGAAAA‑GTGCGGTCGGTTTTGG‑AAA‑TGTTTTTTGAGAACAAAATGAAGGTTCAAACATCCAATGCTTAA‑ACCTGATCAGAACAAGCTGTGaa                                                                                                                                                                                                                                                   <  1:321060/188‑1 (MQ=255)                                                            aTCGAAGGCGCCGGCAACCGCGCCAAATCCACCCTCAGCGAACAGGATATGCAAGATGTGGCAGGCTATGTGGAAACGTTGAAGAAATAATGGAAAA‑GTGCGGTCGGTTTTGG‑AAA‑TGTTTTTTGAGAACAAAATGAAGGTTCAAACATCCAATGCTTAA‑ACCTGATCAGAACAAGCTGTGAATGCTTGCGCTATGCCAAAACAGCAGGGG‑CAGGTCCTGTGCCTGCCCGAATTTTT‑CGATGAATCCAAcg                                                                                                                                                                             >  1:471509/1‑251 (MQ=255)                                                              cGAAGGCGCCGGCAACCGCGCCAAATCCACCCTCAGCGAACAGGATATGCAAGATGTGGCAGGCTATGTGGAAACGTTGAAGAAATAATGGAAAA‑GTGCGGTCGGTTTTGG‑AAA‑TGTTTTTTGAGAACAAAATGAAGGTTCAAACATCCAATGCTTAA‑ACCTGATCAGAACAAGCTGTGAATGCTTGCGCTATGCCAAAACAGCAGGGG‑CAGGTCCTGTGCCTGCCCGAATTTTT‑CGATGAATCCAAcggg                                                                                                                                                                           <  1:645109/251‑1 (MQ=255)                                                                aaGGCGCCGGCAACCGCGCCAAATCCACCCTCAGCGAACAGGATATGCAAGATGTGGCAGGCTATGTGGAAACGTTGAAGAAATAATGGAAAA‑GTGCGGTCGGTTTTGG‑AAA‑TGTTTTTTGAGAACAAAATGAAGGTTCAAACATCCAATGCTTAA‑ACCTGATCAGAACAAGCTGTGAATGCTTGCGCTATGCCAAAACAGCAGGGG‑CAGGTCCTGTGCCTGCCCGAATTTTT‑CGATGAATCCAAcgggcg                                                                                                                                                                         <  1:495031/251‑1 (MQ=255)                                                                     gcCGGCAACCGCGCCAAATCCACCCTCAGCGAACAGGATATGCAAGATGTGGCAGGCTATGTGGAAACGTTGAAGAAATAATGGAAAA‑GTGCGGTCGGTTTTGG‑AAA‑TGTTTTTTGAGAACAAAATGAAGGTTCAAACATCCAATGCTTAA‑ACCTGATCAGAACAAGCTGTGAATg                                                                                                                                                                                                                                                 <  1:117362/176‑1 (MQ=255)                                                                      ccGGCAACCGCGCCAAATCCACCCTCAGCGAACAGGATATGCAAGATGTGGCAGGCTATGTGGAAACGTTGAAGAAATAATGGAAAA‑GTGCGGTCGGTTTTGG‑AAA‑TGTTTTTTGAGAACAAAATGAAGGTTCAAACATCCAATGCTTAA‑ACCTGATCAGAACAAGCTGTGAATGCTTGCGCTATGCCAAAACAGCAGGGG‑CAGGTCCTGTGCCTGCCCGAATTTTT‑CGATGAATCCAACGGGCGGGcac                                                                                                                                                                    <  1:799071/250‑1 (MQ=255)                                                                          cAACCGCGCCAAATCCACCCTCAGCGAACAGGATATGCAAGATGTGGCAGGCTATGTGGAAACGTTGAAGAAATAATGGAAAA‑GTGCGGTCGGTTTTGG‑AAA‑TGTTTTTTGAGAACAAAATGAAGGTTCAAACATCCAATGCTTAA‑ACCTGATCAGAACAAGCTGTGAATGCTTGCGCTATGCCAAAACAGCAGGGG‑CAGGTCCTGTGCCTGCCCGAATTTTT‑CGATGAATCCAACGGGCGGGCACAGGA‑c                                                                                                                                                              <  1:160622/251‑1 (MQ=255)                                                                           aaCCGCGCCAAATCCACCCTCAGCGAACAGGATATGCAAGATGTGGCAGGCTATGTGGAAACGTTGAAGAAATAATGGAAAA‑GTGCGGTCGGTTTTGG‑AAA‑TGTTTTTTGAGAACAAAATGAAGGTTCAAACATCCAATGCTTAA‑ACCTGATCAGAACAAGCTGTGAATGCTTGCGCTATGCCAAAACAGCAGGGG‑CAGGTCCTGTGCCTGCCCGAATTTTT‑CGATGAATCCAACGGGCGGGCACAGGA‑c‑c                                                                                                                                                            <  1:94900/251‑1 (MQ=255)                                                                                  ccAAATCCACCCTCAGCGAACAGGATATGCAAGATGTGGCAGGCTATGTGGAAACGTTGAAGAAATAATGGAAAA‑GTGCGGTCGGTTTTGG‑AAA‑TGTTTTTTGAGAACAAAATGAAGGTTCAAACATCCAATGCTTAA‑ACCTGATCAGAACAAGCTGTGAATGCTTGCGCTATGCCAAAACAGCAGGGG‑CAGGTCCTGTGCCTGCCCGAATTTTT‑CGATGAATCCAACGGGCGGGCACAGGA‑C‑CCGccc                                                                                                                                                       >  1:494638/1‑249 (MQ=255)                                                                                     aaTCCACCCTCAGCGAACAGGATATGCAAGATGTGGCAGGCTATGTGGAAACGTTGAAGAAATAATGGAAAA‑GTGCGGTCGGTTTTGG‑AAA‑TGTTTTTTGAGAACAAAATGAAGGTTCAAACATCCAATGCTTAA‑ACCTGATCAGAACAAGCTGTGAATGCTTGCGCTATGCCAAAACAGCAGGGG‑CAGGTCCTGTGCCTGCCCGAATTTTT‑CGATGAATCCAACGGGCGGGCACAGGA‑c                                                                                                                                                              >  1:490302/1‑240 (MQ=255)                                                                                     aaTCCACCCTCAGCGAACAGGATATGCAAGATGTGGCAGGCTATGTGGAAACGTTGAAGAAATAATGGAAAA‑GTGCGGTCGGTTTTGG‑AAA‑TGTTTTTTGAGAACAAAATGAAGGTTCAAACATCCAATGCTTAA‑ACCTGATCAGAACAAGCTGTGAATGCTTGCGCTATGCCAAAACAGCAGGGG‑CAGGTCCTGTGCCTGCCCGAATTTTT‑CGATGAATCCAACGGGCGGGCACAGGA‑C‑CCGCCCCTAcg                                                                                                                                                  >  1:289406/1‑251 (MQ=255)                                                                                     aaTCCACCCTCAGCGAACAGGATATGCAAGATGTGGCAGGCTATGTGGAAACGTTGAAGAAATAATGGAAAA‑GTGCGGTCGGTTTTGG‑AAA‑TGTTTTTTGAGAACAAAATGAAGGTTCAAACATCCAATGCTTAA‑ACCTGATCAGAACAAGCTGTGAATGCTTGCGCTATGCCAAAACAGCAGGGG‑CAGGTCCTGTGCCTGCCCGAATTTTT‑CGATGAATCCAACGGGCGGGCACAGGA‑C‑CCGCCCCTAcg                                                                                                                                                  >  1:203552/1‑251 (MQ=255)                                                                                               cAGCGAACAGGATATGCAAGATGTGGCAGGCTATGTGGAAACGTTGAAGAAATAATGGAAAA‑GTGCGGTCGGTTTTGG‑AAA‑TGTTTTTTGAGAACAAAATGAAGGTTCAAACATCCAATGCTTAA‑ACCTGATCAGAACAAGCTGTGAATGCt                                                                                                                                                                                                                                               <  1:86868/152‑1 (MQ=255)                                                                                               cAGCGAACAGGATATGCAAGATGTGGCAGGCTATGTGGAAACGTTGAAGAAATAATGGAAAA‑GTGCGGTCGGTTTTGG‑AAA‑TGTTTTTTGAGAACAAAATGAAGGTTCAAACATCCAATGCTTAA‑ACCTGATCAGAACAAGCTGTGAATGCTTGCGCTATGCCAAAACAGCAGGGG‑CAGGTCCTGTGCCTGCCCGAATTTTT‑CGATGAATCCAACGGGCGGGCACAGGA‑C‑CCGCCCCTACGCGAAAAcaca                                                                                                                                        >  1:376389/1‑251 (MQ=255)                                                                                                 gCGAACAGGATATGCAAGATGTGGCAGGCTATGTGGAAACGTTGAAGAAATAATGGAAAA‑GTGCGGTCGGTTTTGG‑AAA‑TGTTTTTTGAGAACAAAATGAAGGTTCAAACATCCAATGCTTAA‑ACCTGATCAGAACAAGCTGTGAATg                                                                                                                                                                                                                                                 >  1:211427/1‑148 (MQ=255)                                                                                                   gAACAGGATATGCAAGATGTGGCAGGCTATGTGGAAACGTTGAAGAAATAATGGAAAA‑GTGCGGTCGGTTTTGG‑AAA‑TGTTTTTTGAGAACAAAATGAAGGTTCAAACATCCAATGCTTAA‑ACCTGATCAGAACAAGCTGTGAATGCTTGCGCTATGCCAAAACAGCAGGGG‑CAGGTCCTGTGCCTGCCCGAATTTTT‑CGATGAATCCAACGGGCGGGCACAGGA‑c                                                                                                                                                              <  1:606436/226‑1 (MQ=255)                                                                                                        ggATATGCAAGATGTGGCAGGCTATGTGGAAACGTTGAAGAAATAATGGAAAA‑GTGCGGTCGGTTTTGG‑AAA‑TGTTTTTTGAGAACAAAATGAAGGTTCAAACATCCAATGCTTAA‑ACCTGATCAGAACAAGCTGTGAATGCTTGCGCTATGCCAAAACAGCAGGGG‑CAGGTCCTGTGCCTGCCCGAATTTTT‑CGATGAATCCAAc                                                                                                                                                                              <  1:307597/206‑1 (MQ=255)                                                                                                        ggATATGCAAGATGTGGCAGGCTATGTGGAAACGTTGAAGAAATAATGGAAAA‑GTGCGGTCGGTTTTGG‑AAA‑TGTTTTTTGAGAACAAAATGAAGGTTCAAACATCCAATGCTTAA‑ACCTGATCAGAACAAGCTGTGAATGCTTGCGCTATGCCAAAACAGCAGGGG‑CAGGTCCTGTGCCTGCCCGAATTTTT‑CGATGAATCCAACGGGCGGGCACAGGA‑C‑CCGCCCCTACGCGAAAACACAAAAAAaca                                                                                                                                >  1:279316/1‑250 (MQ=255)                                                                                                        ggATATGCAAGATGTGGCAGGCTATGTGGAAACGTTGAAGAAATAATGGAAAA‑GTGCGGTCGGTTTTGG‑AAA‑TGTTTTTTGAGAACAAAATGAAGGTTCAAACATCCAATGCTTAA‑ACCTGATCAGAACAAGCTGTGAATGCTTGCGCTATGCCAAAACAGCAGGGG‑CAGGTCCTGTGCCTGCCCGAATTTTT‑CGATGAATCCAACGGGCGGGCACAGGA‑C‑CCGCCCCGAAGCGAAAACACAAAAAAaca                                                                                                                                >  1:559430/1‑250 (MQ=255)                                                                                                            atGCAAGATGTGGCAGGCTATGTGGAAACGTTGAAGAAATAATGGAAAA‑GTGCGGTCGGTTTTGG‑AAA‑TGTTTTTTGAGAACAAAATGAAGGTTCAAACATCCAATGCTTAA‑ACCTGATCAGAACAAGCTGTGAATGCt                                                                                                                                                                                                                                               <  1:557619/139‑1 (MQ=255)                                                                                                            atGCAAGATGTGGCAGGCTATGTGGAAACGTTGAAGAAATAATGGAAAA‑GTGCGGTCGGTTTTGG‑AAA‑TGTTTTTTGAGAACAAAATGAAGGTTCAAACATCCAATGCTTAA‑ACCTGATCAGAACAAGCTGTGAATGCTTGCGCTATGCCAAAACAGCAGGGG‑CAGGTCCTGTGCCTGCCCGAATTTTT‑CGATGAATCCAACGGGCGGGCACAGGA‑C‑CCGcccc                                                                                                                                                      <  1:177763/224‑1 (MQ=255)                                                                                                                 aGATGTGGCAGGCTATGTGGAAACGTTGAAGAAATAATGGAAAA‑GTGCGGTCGGTTTTGG‑AAA‑TGTTTTTTGAGAACAAAATGAAGGTTCAAACATCCAATGCTTAA‑ACCTGATCAGAACAAGCTGTGAATGCTTGCGCTATGCCAAAACAGCAGGGG‑CAGGTCCTGTGCCTGCCCGAATTTTT‑CGATGAATCCAACGGGCGGGCACAGGA‑c                                                                                                                                                              >  1:147856/1‑212 (MQ=255)                                                                                                                 aGATGTGGCAGGCTATGTGGAAACGTTGAAGAAATAATGGAAAA‑GTGCGGTCGGTTTTGG‑AAA‑TGTTTTTTGAGAACAAAATGAAGGTTCAAACATCCAATGCTTAA‑ACCTGATCAGAACAAGCTGTGAATGCTTGCGCTATGCCAAAACAGCAGGGG‑CAGGTCCTGTGCCTGCCCGAATTTTT‑CGATGAATCCAACGGGCGGGCACAGGA‑c                                                                                                                                                              >  1:574127/1‑212 (MQ=255)                                                                                                                 aGATGTGGCAGGCTATGTGGAAACGTTGAAGAAATAATGGAAAA‑GTGCGGTCGGTTTTGG‑AAA‑TGTTTTTTGAGAACAAAATGAAGGTTCAAACATCCAATGCTTAA‑ACCTGATCAGAACAAGCTGTGAATGCTTGCGCTATGCCAAAACAGCAGGGG‑CAGGTCCTGTGCCTGCCCGAATTTTT‑CGATGAATCCAACGGGCGGGCACAGGA‑C‑CCGCCCCTACGCGAAAACACAAAAAAACACCTTTGATAg                                                                                                                      >  1:695978/1‑251 (MQ=255)                                                                                                                   gtggggaaggttATGTGGAAACTTTAAAAAAAAAAGGGAAAA‑GTGCGGT‑GGTTTTGGAAAA‑TTTTTTTTGAAAACAAAATGAAGGTCAAAACACCCAATGCTAAA‑CCCTGATCAGAACAAGCTGTAAATGCTTGCGCTATGCAAAAACAGCGGGGG‑CAGGTCCTGTGCCTGCCCGATTTTTT‑CAATGAATCCAGGGGGCGGGCACAGGA‑C‑CCGCCCCTACGCGAAAACACAAAAAAACACCTTTGATAg                                                                                                                      <  1:21012/238‑1 (MQ=255)                                                                                                                   aTGTGGCAGGTTTTGTAGAAGCTTTAAAAAAAAAATAGAAAT‑GTGCGGTC‑GTTTTGG‑AAATTGTTTTTTAAAAAAAAAAGGAAGTTGCAAACCTCAAATGCTCAA‑TCCTGACCAGAAAAAGCTGAAAATGTTTGCGTTATCCAAAAACAGCGGGGG‑CGGGCCCTTTTCCTCCCCAAATTTTT‑CGATGAACCCACCGGGCGGTAACA‑GA‑CTCCGCCCCTACGCGAAAACACAAAAAAACACCTTTGATAg                                                                                                                      <  1:119310/249‑1 (MQ=255)                                                                                                                              tATGTGGAAACGTTAAAAAAAAAAGGAAAAA‑GTGCGGTCGGTTTTGG‑AAA‑TTTTTTTTGAAAAAAAAATGAGGGTTAAAACATCCAATGCTTAA‑CCCTGATCAGAACAAGCTGTGAATGCTTGCGCTATGCCAAAACAGCAGGGG‑CAGGTCCTGTGCCTGCCCGATTTTTT‑CGATGAACCCAACGGGGGGGCACAGGC‑C‑CCGCCCCTACGCGAAAACACAAAAAAACACCTTTGATAGCGCACGCTTCATa                                                                                                         <  1:426403/251‑1 (MQ=255)                                                                                                                               ttgtgGAAACTTTGAAAAAATAATGGAAAG‑GGGCGGTCGTTTTTGG‑AAA‑TTTTTTTAGAAAACAAAATGAGGGTTCAACCGT‑CAATTGTTAACACCGGACCAGAAAAAGCTGTGAGTCCTTGCGCTACCCAAAAACAGAGGGGG‑CAGGTCGTGTGCCTGCCGGATTTTTT‑CAAT‑AAAACAACCGGGGGGAAACGGAGA‑CCGCCCCTACGCGAAAACACAAAAAAACACCTTTGATAGCGCACGCTTCATAg                                                                                                        <  1:551403/250‑1 (MQ=255)                                                                                                                               ttgggaaaaCGTTGAAAAAATAATGGAAAA‑GTGCGGTCGTTTTTGG‑AAA‑TTTTTTTTGAAAAAAAAATGAGGTTTCAAACATCCAATGCTAAA‑CCCTGATCAGAACAAGCGGTGAATGCTTGCGCTACGCAAAAACAGCGGGGG‑CGGCTCTGGTCCCGGCGAGATTTTTA‑AGATAAAGCCAAGGGGCGGGCAGGGGA‑C‑CCGCCCCTACGCGAAAACACAAAAAAACACCTTTGATAGCGCACGCTTCATAg                                                                                                        <  1:390689/245‑1 (MQ=255)                                                                                                                               aTGTGGAAACGTTGAAGAAATAATGGAAAA‑GTGCGGTCGGTTTTGG‑AAA‑TGTTTTTTGAGAACAAAATGAAGGTTCAAACATCCAATGCTTAA‑ACCTGATCAGAACAAGCTGTGAATGCTTGCGCTATGCCAAAACAGCAGGGG‑CAGGTCCTGTGCCTGCCCGAATTTTT‑CGATGAATCCAACGGGCGGGCACAGGA‑C‑CTGCCCCtgctgttttggcat                                                                                                                                        <  1:262049/219‑14 (MQ=255)                                                                                                                               aTGTGGAAACGTTGAAGAAATAATGGAAAA‑GTGCGGTCGGTTTTGG‑AAA‑TGTTTTTTGAGAACAAAATGAAGGTTCAAACATCCAATGCTTAA‑ACCTGATCAGAACAAGCTGTGAATGCTTGCGCTATGCCAAAACAGCAGGGG‑CAGGTCCTGTGCCTGCCCGAATTTTT‑CGATGAATCCAACGGGCGGGCACAGGA‑C‑CCGCCCCTACGCGAAAACACAAAAAAACACCTTTGATAGCGCACGCTTCATAg                                                                                                        >  1:741888/1‑251 (MQ=255)                                                                                                                                tgtggaatcgtcaaaaaaaTAAGGGAAAG‑GTGCGGCAGGTTTTGG‑AAA‑TTTTTTTAGAGAACAAAATGAAGTTTAAAACACCAAAGGCTTAA‑CCCTAACCAGAACAAGTTGTGAGTGCTTGGGTTATCCAAAAACA‑AAGGGGACGGGCCCTTGGCCTG‑CCGAATTTTTGGGATAAACCCAGGGGGGGCGCACAGGA‑C‑CCGCCCCTACGCGAAAACACAAAAAAACGCCTTTGATAGCGCACGCTTCATAgc                                                                                                       <  1:803106/235‑1 (MQ=255)                                                                                                                                tgtgaaaaggttaaaaaaaTAAGGAAAAA‑GGGCGGTCGGTTTTGG‑AAT‑TGTTTTTTGAGAAAAAAATGAAGGTTCAAACATCCAATGCTAAA‑CCCTGATCAGAACAAGCTGTGAATGCTTGCGCTATGCCAAAACAGCGGGGG‑CAGGTCCTGTGCCTGCCCGAATTTTT‑CGATAAATCCAACGGGCGGGCACAGGA‑C‑CCGCCCCTACGCGAAAACACAAAAAAACACCTTTGATAGCGCACGCTTCATAgc                                                                                                       <  1:660838/235‑1 (MQ=255)                                                                                                                                tgtgAAAACGTTAAAGAAATAA‑GGAAAAGGTGCGGTCGGTTTTGA‑AAT‑TTTTTTTTGAGAAAAAAATGAAGTTTCAAACATCAAATGCTAAA‑CCCTGATCAGAACAGGTTGTGAATGCTTGCGCTATGCAAAAACAGCGGGGG‑CAGGTCCTGTGCCT‑CCCCAATTTTTCCGATAAATCCAACGGGCGGGCACGGGA‑C‑CCGCCCCTACGCGAAAACACAAAAAAACACCTTTGATAGCGCACGCTTCATAgc                                                                                                       <  1:282356/251‑1 (MQ=255)                                                                                                                                  ggaaaaCGTAGAAAAAAAAATGAAAAA‑GGGCGTTCGGTTTGGA‑AAA‑TTTTTTTTGAGAAAAAAATGAAGGTCAAAACACCCAATGCTTAA‑CCCTGATCAGAACAAGCTGTGAATGCTTGCGCTATGCCAAAACAGCGGGGG‑CAGGTCCTGTGCCTGCCCGATTTTTT‑CGATAAACCCAACGGGCGGGCACAGGA‑C‑CCGCCCCTACGCGAAAACACAAAAAAACACCTTTGATAGCGCACGCTTCATAgcg                                                                                                      <  1:574506/247‑1 (MQ=255)                                                                                                                                        cGTTGAAGAAATAATGGAAAA‑GTGCGGTCGGTTTTGG‑AAA‑TGTTTTTTGAGAACAAAATGAAGGTTCAAACATCCAATGCTTAA‑ACCTGATCAGAACAAGCTGTGAATGCTTGCGCTATGCCAAAACAGCAGGGG‑CAGGTCCTGTGCCTGCCCGAATTTTT‑CGATGAATCCAAcggg                                                                                                                                                                           >  1:13680/1‑177 (MQ=255)                                                                                                                                             aaaaaaTAATGGAAAA‑GTGGGGTGGTTTTTGG‑AAA‑TGTTTTTGGAAAAAAAAATGAAGGTTCAAACACCAAATGCTTAA‑CCCTGATCAGAACAAGCTGTGAATGCTTGCGCTATGCAAAAACAGCGGGGG‑CAGGTCCTGTGCCTGCCCGATTTTTT‑CGATGAATCCAACGGGCGGGCACAGGA‑C‑CCGCCCCTACGCGAAAACACAAAAAAACACCTTTGATAGCGCACGCTTCATAGCGCGTGCTTTTAAc                                                                                          <  1:208927/248‑1 (MQ=255)                                                                                                                                                     atgaaaaa‑GTGCGGTGGGTTTTGA‑AAA‑TTTTTTTTGAAAACAAAAGGAGGGTTAAAACATCAAATGCTAAA‑CCCTGATCAGAACAAGCTGTGAATGCTTGCGCTATGCAAAAACAGCGGGGG‑CGGGTCCTGGGCCTGCCCAATTTTTT‑CGATGAACCCAACGGGCGGGCACGGGA‑C‑CCGCCCCTACGCGAAAACACAAAAAAACACCTTTGAt                                                                                                                        <  1:727740/209‑1 (MQ=255)                                                                                                                                                       ggAAAA‑GTGCGGTCGGTTTTGG‑AAA‑TGTTTTTTGAGAACGAAATGAAGGTTCAAACATCCAATGCTTAA‑ACCTGATCAGAACAAGCTGTGAATGCTTGCGCTATGCCAAAACAGCAGGGG‑CAGGTCCTGTGCCTGCCCGAATTTTT‑CGATGAATCCAACGGGCGGGCACAGGA‑C‑CCGCCCCTACGCGAAAACACAAAAAAACACCTTTGATAGCGCACGCTTCATAGCGCGTGCTTTTAACTTATTGatat                                                                                >  1:123244/1‑251 (MQ=255)                                                                                                                                                               tggggtcgttTTTGG‑AAT‑TTTTTTTTGAGAAAAAAATGAAGGTTCAAACATCCAATGCTTAA‑ACCTGATCAGAACAGGCTGTGAATGCTTGCGCTATGCCAAAACGGCAGGGG‑CAGGTCCTGTGCCTGCCCAATTTTTT‑CGATGAACCCACCGGGCGGGCACAGGA‑C‑CCGCCCCTACGCGAAAACACAAAAAAACACCTTTGATAGCGCACGCTTCATAGCGCGTGCTTTTAACTTATTGATATTACAgg                                                                          <  1:796962/247‑1 (MQ=255)                                                                                                                                                                             g‑AAA‑TGTTTTTTGAGAACAAAATGAAGGTTCAAACATCCAATGCTTAA‑ACCTGATCAGAACAAGCTGTGAATGCTTGCGCTATGCCAAAACAGCAGGGG‑CAGGTCCTGTGCCTGCCCGAATTTTT‑CGATGAATCCAACGGGCGGGCACAGGA‑C‑CCGCCCCTACGCGAAAACACAAAAAAACACCTTTGATAGCGCACGCTTCATAGCGCGTGCTTTTAACTTATTGATATTACAGGCACGCGTTAGGaaa                                                            >  1:520299/1‑250 (MQ=255)                                                                                                                                                                                    gTTTTTTGAGAACAAAATGAAGGTTCAAACATCCAATGCTTAA‑ACCTGATCAGAACAAGCTGTGAATGCTTGCGCTATGCCAAAACAGCAGGGG‑CAGGTCCTGTGCCTGCCCGAATTTTT‑CGATGAATCCAACGGGCGGGCACAGGA‑C‑CCGCCCCTACGCGAAAACACAAAAAAACACCTTTGATAGCGCACGCTTCATAgc                                                                                                       >  1:316204/1‑202 (MQ=255)                                                                                                                                                                                             gaACAAAATGAAGGTTCAAACATCCAATGCTTAA‑ACCTGATCAGAACAAGCTGTGAATGCTTGCGCTATGCCAAAACAGCAGGGG‑CAGGTCCTGTGCCTGCCCGAATTTTT‑CGATGAATCCAACGGGCGGGCACAGGA‑C‑CCGCCCCTACGCGAAAACACAAAAAAACACCTTTGATAGCGCACGCTTCATAGCGCGTGCTTTTAACTTATTGATATTACAGGCACGCGTTAGGAAACGCGCACCAGCaaaa                                             >  1:242446/1‑251 (MQ=255)                                                                                                                                                                                                  aaaTGAAGGTTCAAACATCCAATGCTTAA‑ACCTGATCAGAACAAGCTGTGAATGCTTGCGCTATGCCAAAACAGCAGGGG‑CAGGTCCTGTGCCTGCCCGAATTTTT‑CGATGAATCCAACGGGCGGGCACAGGA‑C‑CCGCCCCTACGCGAAAACACAAAAAAACACCTTTGATAGCGCACGCTTCATAGCGCGTGCTTTTAACTTATTGATATTACAGGCAcgcg                                                                    >  1:67361/1‑223 (MQ=255)                                                                                                                                                                                                             cAAACATCCAATGCTTAA‑ACCTGATCAGAACAAGCTGTGAATGCTTGCGCTATGCCAAAACAGCAGGGG‑CAGGTCCTGTGCCTGCCCGAATTTTT‑CGATGAATCCAACGGGCGGGCACAGGA‑C‑CCGCCCCTACGCGAAAACACAAAAAAACACCTTTGATAGCGCACGCTTCATAGCGCGTGCTTTTAACTTATTGATATTACAGGCACGCGTTAGGAAACGCGCACCAGCAAAATGTAACGTTTTTCaaa                             >  1:756043/1‑251 (MQ=255)                                                                                                                                                                                                             cAAACATCAAATGCTAAA‑ACCTGATCAGAACAGGCTGTGAATGCTTGCGTTATGCAAAAACAGCGGGGG‑CAGGTCCTGTGCCTGCCCGATTTTTT‑CGATGAATCCAACGGGCGGGCACAGGA‑C‑CCGCCCCTACGCGAAAACACAAAAAAACACCTTTGAt                                                                                                                        <  1:547767/160‑1 (MQ=255)                                                                                                                                                                                                                 cttCCAATGCTTAA‑ACCTGATCAGAACAAGCTGTGAATGCTTGCGCTATGCCAAAACAGCAGGGG‑CAGGTCCTGTGCCTGCCCGAATTTTT‑CGATGAATCCAACGGGCGGGCACAGGA‑C‑CCGcc                                                                                                                                                        <  1:243203/122‑1 (MQ=255)                                                                                                                                                                                                                         gCTTAA‑ACCTGATCAGAACAAGCTGTGAATGCTTGCGCTATGCCAAAACAGCAGGGG‑CAGGTCCTGTGCCTGCCCGAATTTTT‑CGATGAATCCAACGGGCGGGCACAGGA‑C‑CCGcccc                                                                                                                                                      >  1:149391/1‑118 (MQ=255)                                                                                                                                                                                                                         gCTTAA‑ACCTGATCAGAACAAGCTGTGAATGCTTGCGCTATGCCAAAACAGCAGGGG‑CAGGTCCTGTGCCTGCCCGAATTTTT‑CGATGAATCCAACGGGCGGGCACAGGA‑C‑CCGCCCCTACGCGAAAACACAAAAAAACACCTTTGATAGCGCACGCTTCATAGCGCGTGCTTTTAACTTATTGATATTACAGGCACGCGTTAGGAAACGCGCACCAGCAAAATGTAACGTTTTTCAAAATTCAACTGTCa                 >  1:560495/1‑251 (MQ=255)                                                                                                                                                                                                                              a‑aCCTGATCAGAACAAGCTGTGAATGCTTGCGCTATGCCAAAACAGCGGGGG‑CAGGTCCTGTGCCTGCCCGAATTTTT‑CGATGAATCCAACGGGCGGGCACAGGA‑C‑CCGCCCCTACGCGAAAACACAAAAAAACACCTTTGATAg                                                                                                                      >  1:432288/1‑145 (MQ=255)                                                                                                                                                                                                                              a‑aCCTGATCAGAACAAGCTGTGAATGCTTGCGCTATGCCAAAACAGCGGGGG‑CAGGTCCTGTGCCTGCCCGAATTTTT‑CGATAAACCCAACGGGCGGGCACAGGA‑C‑CCGCCCCTACGCGAAAACACAAAAAAACACCTTTGATAGCGCACGCTTCATAGCGCGTGCTTTTAACTTATTGATATTACAGGCACGCGTTAGGAAACGCGCACCAGCAAAATGTAACGTTTTTCAAAATTCAACTGTCATAAgg            <  1:509388/251‑1 (MQ=255)                                                                                                                                                                                                                              a‑aCCTGATCAGAACAAGCTGTGAATGCTTGCGCTATGCAAAAACAGCGGGGG‑CAGGTCCTGTGCCTGCCCGAATTTTT‑CGATGAATCCAACGGGCGGGCACAGGA‑C‑CCGCCCCTACGCGAAAACACAAAAAAACACCTTTGATAGCGCACGCTTCATAGCGCGTGCTTTTAACTTATTGATATTACAGGCACGCGTTAGGAAACGCGCACCAGCAAAATGTAACGTTTTTCAAAATTCAACTGTCATAAgg            <  1:302490/251‑1 (MQ=255)                                                                                                                                                                                                                                    gATCAGAACAAGCTGTGAATGCTTGCGCTATGCCAAAACAGCAGGGG‑CAGGTCCTGTGCCTGCCCGAATTTTT‑CGATGAATCCAACGGGCGGGCACAGGA‑C‑CCGCCCCTACGCGAAAACACAAAAAAACACCTTTGATAGCGCACGCTTCATAGCGCGTGCTTTTAACTTATTGATATTACAGGCACGCGTTAGGAAACGCGCACCAGCAAAATGTAACGTTTTTCAAAATTCAACTGTCATAAgg            >  1:880841/1‑246 (MQ=255)                                                                                                                                                                                                                                         gAACAAGCTGTGAATGCTTGCGCTATGCCAAAACAGCAGGGG‑CAGGTCCTGTGCCTGCCCGAATTTTT‑CGATGAATCCAACGGGCGGGCACAGGA‑C‑CCGCCCCTACGCGAAAACACAAAAAAACACCTTTGATAGCGCACGCTTCATAGCGCGTGCTTTTAACTTATTGATATTACAGGCACGCGTTAGGAAACGCGCACCAGCAAAATGTAACGTTTTTCAAAATTCAACTGTCATAAGGAAACTCTATg  >  1:573828/1‑251 (MQ=255)                                                                                                                                                                                                                                          aaCAAGCTGTGAATGCTTGCGCTATGCCAAAACAGCAGGGG‑CAGGTCCTGTGCCTGCCCGAATTTTT‑CGATGAATCCAACGGGCGGGCACAGGA‑C‑CCGCCCCTACGCGAAAACACAAAAAAACACCTTTGATAGCGCACGCTTCATAGCGCGTGCTTTTAACTTATTGATATTACAGGCACGCGTTAGGAAACGCGCACCAGCaa                                               >  1:639449/1‑205 (MQ=255)                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                      TCAACACCTTCACCCAAGACCAAATCATCAACGCTCTGCAAGACCGCAAGAGCGGTAAAATCGAAGGCGCCGGCAACCGCGCCAAATCCACCCTCAGCGAACAGGATATGCAAGATGTGGCAGGCTATGTGGAAACGTTGAAGAAATAATGGAAAA‑GTGCGGTCGGTTTTGG‑AAA‑TGTTTTTTGAGAACAAAATGAAGGTTCAAACATCCAATGCTTAA‑ACCTGATCAGAACAAGCTATGAATGCTTGCGCTATGCCAAAACAGCAGGGG‑CAGGTCCTGTGCCTGCCCGAATTTTT‑CGATGAATCCAACGGGCGGGCACAGGA‑C‑CCGCCCCTACGCGAAAACACAAAAAAACACCTTTGATAGCGCACGCTTCATAGCGCGTGCTTTTAACTTATTGATATTACAGGCACGCGTTAGGAAACGCGCACCAGCAAAATGTAACGTTTTTCAAAATTCAACTGTCATAAGGAAACTCTATG  >  1/1687380‑1687858

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑