Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,685,830	C→A	intergenic (‑15882/‑34053)	unknown ← / → unknown	tRNA‑Arg‑CCG/tRNA‑Asn‑GTT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,685,830	0	C	A	100.0%	211.5 / NA	76	intergenic (‑15882/‑34053)	unknown/unknown	tRNA‑Arg‑CCG/tRNA‑Asn‑GTT
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (35/41);  total (35/41)

GCAACTTCAGCAACAATATCCCGATTTGGATATTCATCCGATCCGCAAAGTCTCCGCCTCCGAAGGGCAAATTCTGGATAAGATTAAAGGCTTGATGGGCTTGATTTCCGTGGTGATTCTGATTTTAGCCACCCTCTGCGTGAACACCACTCTCATCGCCATCGTGGGCGAACGGGCAAAAGAATTCGCGTTGCAAAAAGCCCTCGGCGCGAAAAGCCGGGACATCATCAAACAAATCGGCGCGGAAACCTTAATTATCGCCGTATGCGCGATCATCAGCGGCTTAATTATCGGTTATTTACTGGCGCAAGTACTCGGCTTAA‑CCGTCTTCAAAGCCTACATCGACATGCGCCTGCCGGTGTTGCCGATCACCATCGGATTATCCTTATTAGTGGCGTTTATCGCAGTTATCGTACCGACCCGCCGCGCCTTAGACATTCAAACCGCCAATGTGTTAAAAGGGGAATAACATGGCTAAAAC  >  1/1685588‑1686068                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                gCAACTTCAGCAACAATATCCCGATTTGGATATTCATCCGATCCGCAAAGTCTCCGCCTCCGAAGGGCAAATTCTGGATAAGATTAAAGGCTTGATGGGCTTGATTTCCGTGGTGATTCTGATTTTAGCCACCCTCTGCGTGAACACCACTCTCATCGCCATCGTGGGCGAACGGGCAAAAGAATTCGCGTTGCAAAAAGCCCTCGGCGCGAAAAGCCGGGACATCATCAAACAAATCGGCGAGGAAAc                                                                                                                                                                                                                                           >  1:811653/1‑249 (MQ=255)      ttCAGCAACAATATCCCGATTTGGATATTCATCCGATCCGCAAAGTCTCCGCCTCCGAAGGGCAAATTCTGGATAAGATTAAAGGCTTGATGGGCTTGATTTCCGTGGTGATTCTGATTTTAGCCACCCTCTGCGTGAACACCACTCTCATCGCCATCGTGGGCGAACGGGCAAAAGAATTCGCGTTGCAAAAAGCCCTCGGCGCGAAAAGCCGGGACATCATCAAACAAATCGGCGAGGAAACCTTAAtt                                                                                                                                                                                                                                    <  1:476428/251‑1 (MQ=255)       tCAGCAACAATATCCCGATTTGGATATTCATCCGATCCGCAAAGTCTCCGCCTCCGAAGGGCAAATTCTGGATAAGATTAAAGGCTTGATGGGCTTGATTTCCGTGGTGATTCTGATTTTAGCCACCCTCTGCGTGAACACCACTCTCATCGCCATCGTGGGCGAACGGGCAAAAGAATTCGCGTTGCAAAAAGCCCTCGGCGCGAAAAGCCGGGACATCATCAAACAAATCGGCGAGGAAACCTTAATTa                                                                                                                                                                                                                                   <  1:293969/251‑1 (MQ=255)       tCAGCAACAATATCCCGATTTGGATATTCATCCGATCCGCAAAGTCTCCGCCTCCGAAGGGCAAATTCTGGATAAGATTAAAGGCTTGATGGGCTTGATTTCCGTGGTGATTCTGATTTTAGCCACCCTCTGCGTGAACACCACTCTCATCGCCATCGTGGGCGAACGGGCAAAAGAATTCGCGTTGCAAAAAGCCCTCGGCGCGAAAAGCCGGGACATCATCAAACAAATCGGCGAGGAAACCTTAATTa                                                                                                                                                                                                                                   <  1:261592/251‑1 (MQ=255)       tCAGCAACAATATCCCGATTTGGATATTCATCCGATCCGCAAAGTCTCCGCCTCCGAAGGGCAAATTCTGGATAAGATTAAAGGCTTGATGGGCTTGATTTCCGTGGTGATTCTGATTTTAGCCACCCTCTGCGTGAACACCACTCTCATCGCCATCGTGGGCGAACGGGCAAAAGAATTCGCGTTGCAAAAAGCCCTCGGCGCGAAAAGCCGGGACATCATCAAACAAATCGGCGAGGAAACCTTAATTa                                                                                                                                                                                                                                   <  1:221708/251‑1 (MQ=255)       tCAGCAACAATATCCCGATTTGGATATTCATCCGATCCGCAAAGTCTCCACCTCCGAAGGGCAAATTCTGGATAAGATTAAAGGCTTGATGGGCTTGATTTCCGTGGTGATTCTGATTTTAGCCACCCTCTGCGTGAACACCACTCTCATCGCCATCGTGGGCGAACGGGCAAAAGAATTCGCGTTGCAAAAAGCCCTCGGCGCGAAAAGCCGGGACATCATCAAACAAATCGGCGAGGAAACCGTAATTa                                                                                                                                                                                                                                   <  1:306263/251‑1 (MQ=255)        cAGCAACAATATCCCGATTTGGATATTCATCCGATCCGCAAAGTCTCCGCCTCCGAAGGGCAAATTCTGGATAAGATTAAAGGCTTGATGGGCTTGATTTCCGTGGTGATTCTGATTTTAGCCACCCTCTGCGTGAACACCACTCTCATCGCCATCGTGGGCGAACGGGCAAAAGAATTCGCGTTGCAAAAAGCCCTCGGCGCGAAAAGCCGGGACATCATCAAACAAATCGGCGAGGAAACCTTAATTAt                                                                                                                                                                                                                                  >  1:407997/1‑251 (MQ=255)        cAGCAACAATATCCCGATTTGGATATTCATCCGATCCGCAAAGTCTCCGCCTCCGAAGGGCAAATTCTGGATAAGATTAAAGGCTTGATGGGCTTGATTTCCGTGGTGATTCTGATTTTAGCCACCCTCTGCGTGAACACCACTCTCATCGCCATCGTGGGCGAACGGGCAAAAGAATTCGCGTTGCAAAAAGCCCTCGGCGCGAAAAGCCGGGACATCATCAAACAAATCGGCGAGGAAACCTTAATTAt                                                                                                                                                                                                                                  >  1:38235/1‑251 (MQ=255)              caaTATCCCGATTTGGATATTCATCCGATCCGCAAAGTCTCCGCCTCCGAAGGGCAAATTCTGGATAAGATTAAAGGCTTGATGGGCTTGATTTCCGTGGTGATTCTGATTTTAGCCACCCTCTGCGTGAACACCACTCTCATCGCCATCGTGGGCGAACGGGCAAAAGAATTCGCGTTGCAAAAAGCCCTCGGCGCGAAAAGCCGGGACATCATCAAACAAATCGGCGAGGAAACCTTAAtt                                                                                                                                                                                                                                    >  1:189280/1‑243 (MQ=255)                 tatCCCGATTTGGATATTCATCCGATCCGCAAAGTCTCCGCCTCCGAAGGGCAAATTCTGGATAAGATTAAAGGCTTGATGGGCTTGATTTCCGTGGTGATTCTGATTTTAGCCACCCTCTGCGTGAACACCACTCTCATCGCCATCGTGGGCGAACGGGCAAAAGAATTCGCGTTGCAAAAAGCCCTCGGCGCGAAAAGCCGGGACATCATCAAACAAATCGGCGAGGAAACCTTAATTATCGCCGTATg                                                                                                                                                                                                                         <  1:273287/251‑1 (MQ=255)                  atCCCGATTTGGATATTCATCCGATCCGCAAAGTCTCCGCCTCCGAAGGGCAAATTCTGGATAAGATTAAAGGCTTGATGGGCTTGATTTCCGTGGTGATTCTGATTTTAGCCACCCTCTGCGTGAACACCACTCTCATCGCCATCGTGGGCGAACGGGCAAAAGAATTCGCGTTGCAAAAAGCCCTCGGCGCGAAAAGCCGGGACATCATCAAACAAATCGGCGAGGAAACCTTAATTATCGCCGTATg                                                                                                                                                                                                                         >  1:10847/1‑250 (MQ=255)                   tCCCGATTTGGATATTCATCCGATCCGCAAAGTCTCCGCCTCCGAAGGGCAAATTCTGGATAAGATTAAAGGCTTGATGGGCTTGATTTCCGTGGTGATTCTGATTTTAGCCACCCTCTGCGTGAACACCACTCTCATCGCCATCGTGGGCGAACGGGCAAAAGAATTCGCGTTGCAAAAAGCCCTCGGCGCGAAAAGCCGGGACATCATCAAACAAATCGGCGAGGAAACCTTAATTATCGCCGTATgcg                                                                                                                                                                                                                       <  1:101337/251‑1 (MQ=255)                             gATATTCATCCGATCCGCAAAGTCTCCGC‑‑CCGAAGGGCAAATTCTGGATAAGATTAAAGGCTTGATGGGCTTGATTTCCGTGGTGATTCTGATTTTAGCCACCCTCTGCGTGAACACCACTCTCATCGCCATCGTGGGCGAACGGGCAAAAGAATTCGCGTTGCAAAAAGCCCTCGGCGCGAAAAGCCGGGACATCATCAAACAAATCGGCGAGGAAACCTTAATTATCGCCGTATGCGCGATCATCAGc                                                                                                                                                                                                            <  1:212420/250‑1 (MQ=255)                                atTCATCCGATCCGCAAAGTCTCCGCCTCCGAAGGGCAAATTCTGGATAAGATTAAAGGCTTGATGGGCTTGATTTCCGTGGTGATTCTGATTTTAGCCACCCTCTGCGTGAACACCACTCTCATCGCCATCGTGGGCGAACGGGCAAAAGAATTCGCGTTGCAAAAAGCCCTCGGCGCGAAAAGCCGGGACATCATCAAACAAATCGGCGAGGAAACCTTAATTATCGCCGTATGCGCGATCATCAGCgg                                                                                                                                                                                                          <  1:84185/251‑1 (MQ=255)                                   cATCCGATCCGCAAAGTCTCCGCCTCCGAAGGGCAAATTCTGGATAAGATTAAAGGCTTGATGGGCTTGATTTCCGTGGTGATTCTGATTTTAGCCACCCTCTGCGTGAACACCACTCTCATCGCCATCGTGGGCGAACGGGCAAAAGAATTCGCGTTGCAAAAAGCCCTCGGCGCGAAAAGCCGGGACATCATCAAACAAATCGGCGAGGAAACCTTAATTATCGCCGTATGCGCGATCATCAGCGGCtt                                                                                                                                                                                                       >  1:608358/1‑251 (MQ=255)                                              cAAAGTCTCCGCCTCCGAAGGGCAAATTCTGGATAAGATTAAAGGCTTGATGGGCTTGATTTCCGTGGTGATTCTGATTTTAGCCACCCTCTGCGTGAACACCACTCTCATCGCCATCGTGGGCGAACGGGCAAAAGAATTCGCGTTGCAAAAAGCCCTCGGCGCGAAAAGCCGGGACATCATCAAACAAATCGGCGAGGAAACCTTAATTATCGCCGTATGCGCGATCATCAGCGGCTTAATTATCGGt                                                                                                                                                                                             <  1:814636/250‑1 (MQ=255)                                                  gTCTCCGCCTCCGAAGGGCAAATTCTGGATAAGATTAAAGGCTTGATGGGCTTGATTTCCGTGGTGATTCTGATTTTAGCCACCCTCTGCGTGAACACCACTCTCATCGCCATCGTGGGCGAACGGGCAAAAGAATTCGCGTTGCAAAAAGCCCTCGGCGCGAAAAGCCGGGACATCATCAAACAAATCGGCGAGGAAACCTTAATTa                                                                                                                                                                                                                                   <  1:30370/208‑1 (MQ=255)                                                   tctcCGCCTCCGAAGGGCAAATTCTGGATAAGATTAAAGGCTTGATGGGCTTGATTTCCGTGGTGATTCTGATTTTAGCCACCCTCTGCGTGAACACCACTCTCATCGCCATCGTGGGCGAACGGGCAAAAGAATTCGCGTTGCAAAAAGCCCTCGGCGCGAAAAGCCGGGACATCATCAAACAAATCGGCGAGGAAACCTTAATTATCGCCGTATGCGCGATCATCAGCGGCTTAATTATCGGTTAttt                                                                                                                                                                                        <  1:110398/250‑1 (MQ=255)                                                    ctcCGCCTCCGAAGGGCAAATTCTGGATAAGATTAAAGGCTTGATGGGCTTGATTTCCGTGGTGATTCTGATTTTAGCCACCCTCTGCGTGAACACCACTCTCATCGCCATCGTGGGCGAACGGGCAAAAGAATTCGCGTTGCAAAAAGCCCTCGGCGCGAAAAGCCGGGACATCATCAAACAAATCGGCGAGGAAACCTTAATTATCGCCGTATGCGCGATCATCAGCGGCTTAATTATCGGTTATTTAc                                                                                                                                                                                      <  1:656902/251‑1 (MQ=255)                                                    ctcCGCCTCCGAAGGGCAAATTCTGGATAAGATTAAAGGCTTGATGGGCTTGATTTCCGTGGTGATTCTGATTTTAGCCACCCTCTGCGTGAACACCACTCTCATCGCCATCGTGGGCGAACGGGCAAAAGAATTCGCGTTGCAAAAAGCCCTCGGCGCGAAAAGCCGGGACATCATCAAACAAATCGGCGAGGAAACCTTAATTATCGCCGTATGCGCGATCATCAGCGGCTTAATTATCGGTTATTTAc                                                                                                                                                                                      <  1:355822/251‑1 (MQ=255)                                                               aaGGGCAAATTCTGGATAAGATTAAAGGCTTGATGGGCTTGATTTCCGTGGTGATTCTGATTTTAGCCACCCTCTGCGTGAACACCACTCTCATCGCCATCGTGGGCGAACGGGCAAAAGAATTCGCGTTGCAAAAAGCCCTCGGCGCGAAAAGCCGGGACATCATCAAACAAATCGGCGAGGAAACCTTAATTATCGCCGTATGCGCGATCATCAGCGGCTTAATTATCGGTTATTTACTGGCGCAAGt                                                                                                                                                                            <  1:154673/250‑1 (MQ=255)                                                                  ggCAAATTCTGGATAAGATTAAAGGCTTGATGGGCTTGATTTCCGTGGTGATTCTGATTTTAGCCACCCTCTGCGTGAACACCACTCTCACCGCCATCGTGGGCGAACGGGCAAAAGAATTCGCGTTGCAAAAAGCCCTCGGCGCGAAAAGCCGGGACATCATCAAACAAATCGGCGAGGAAACCTTAATTATCGCCGTATGCGCGATCATCAGCGGCTTAATTATCGGTTATTTACTGGCGCAAGTAc                                                                                                                                                                          <  1:514015/249‑1 (MQ=255)                                                                            ggATAAGATTAAAGGCTTGATGGGCTTGATTTCCGTGGTGATTCTGATTTTAGCCACCCTCTGCGTGAACACCACTCTCATCGCCATCGTGGGCGAACGGGCAGAAGAATTCGCGTTGCAAAAAGCCCTCGGCGCGAAAAGCCGGGACATCATCAAACAAATCGGCGAGGAAACCTTAATTATCGCCGTATGCGCGATCATCAGCGGCTTAATTATCGGTTATTTACTGGCGCAAGTACTCGGCTTAA‑CCg                                                                                                                                                             >  1:16967/1‑251 (MQ=255)                                                                            ggATAAGATTAAAGGCTTGATGGGCTTGATTTCCGTGGTGATTCTGATTTTAGCCACCCTCTGCGTGAACACCACTCTCATCGCCATCGTGGGCGAACGGGCAAAAGAATTCGCGTTGCAAAAAGCCCTCGGCGCGAAAAGCCGGGACATCATCAAACAAATCGGCGAGGAAACCTTAATTATCGCCGTATGCGCGATCATCAGCGGCTTAATTATCGGt                                                                                                                                                                                             >  1:385570/1‑220 (MQ=255)                                                                            ggATAAGATTAAAGGCTTGATGGGCTTGATTTCCGTGGTGATTCTGATTTTAGCCACCCTCTGCGTGAACACCACTCTCATCGCCATCGTGGGCGAACGGGCAAAAGAATTCGCGTTGCAAAAAGCCCTCGGCGCGAAAAGCCGGGACATCATCAAACAAATCGGCGAGGAAACCTTAATTATCGCCGTATGCGCGATCATCAGCGGCTTAATTATCGGTTATTTACTGGCGCAAGTACTCGGCTTAA‑CCg                                                                                                                                                             >  1:82870/1‑251 (MQ=255)                                                                                 aGATTAAAGGCTTGATGGGCTTGATTTCCGTGGTGATTCTGATTTTAGCCACCCTCTGCGTGAACACCACTCTCATCGCCATCGTGGGCGAACGGGCAAAAGAATTCGCGTTGCAAAAAGCCCTCGGCGCGAAAAGCCGGGACATCATCAAACAAATCGGCGAGGAAACCTTAATTATCGCCGTATg                                                                                                                                                                                                                         >  1:755528/1‑187 (MQ=255)                                                                                  gATTAAAGGCTTGATGGGCTTGATTTCCGTGGTGATTCTGATTTTAGCCACCCTCTGCGTGAACACCACTCTCATCGCCATCGTGGGCGAACGGGCAAAAGAATTCGCGTTGCAAAAAGCCCTCGGCGCGAAAAGCCGGGACATCATCAAACAAATCGGCGAGGAAACCTTAATTATCGCCGTATgcg                                                                                                                                                                                                                       >  1:599162/1‑188 (MQ=255)                                                                                  gATTAAAGGCTTGATGGGCTTGATTTCCGTGGTGATTCTGATTTTAGCCACCCTCTGCGTGAACACCACTCTCATCGCCATCGTGGGCGAACGGGCAAAAGAATTCGCGTTGCAAAAAGCCCTCGGCGCGAAAAGCCGGGACATCATCAAACAAATCGGCGAGGAAACCTTAATTATCGCCGTATGCGCGATCATCAGCGGCTTAATTATCg                                                                                                                                                                                               >  1:270322/1‑212 (MQ=255)                                                                                  gATTAAAGGCTTGATGGGCTTGATTTCCGTGGTGATTCTGATTTTAGCCACCCTCTGCGTGAACACCACTCTCATCGCCATCGTGGGCGAACGGGCAAAAGAATTCGCGTTGCAAAAAGCCCTCGGCGCGAAAAGCCGGGACATCATCAAACAAATCGGCGAGGAAACCTTAATTATCGCCGTATGCGCGATCATCAGCGGCTTAATTATCGGTTATTTAc                                                                                                                                                                                      <  1:751479/221‑1 (MQ=255)                                                                                  gATTAAAGGCTTGATGGGCTTGATTTCCGTGGTGATTCTGATTTTAGCCACCCTCTGCGTGAACACCACTCTCATCGCCATCGTGGGCGAACGGGCAAAAGAATTCGCGTTGCAAAAAGCCCTCGGCGCGAAAAGCCGGGACATCATCAAACAAATCGGCGAGGAAACCTTAATTATCGCCGTATGCGCGATCATCAGCGGCTTAATTATCGGTTATTTACTGGCGCAAGTACTCGGCTTAA‑CCGTCTTCa                                                                                                                                                       >  1:329581/1‑251 (MQ=255)                                                                                   aTTAAAGGCTTGATGGGCTTGATTTCCGTGGTGATTCTGATTTTAGCCACCCTCTGCGTGAACACCACTCTCATCGCCATCGTGGGCGAACGGGCAAAAGAATTCGCGTTGCAAAAAGCCCTCGGCGCGAAAAGCCGGGACATCATCAAACAAATCGGCGAGGAAACCTTAATTATCGCCGTATGCGCGATCATCAGCGGCTTAATTATCGGTTATTTACTGGCGCAAGTACTCGGCTTAA‑CCGTCTTCaa                                                                                                                                                      >  1:311191/1‑251 (MQ=255)                                                                                      aaaGGCTTGATGGGCTTGATTTCCGTGGTGATTCTGATTTTAGCCACCCTCTGCGTGAACACCACTCTCATCGCCATCGTGGGCGAACGGGCAAAAGAATTCGCGTTGCAAAAAGCCCTCGGCGCGAAAAGCCGGGACATCATCAAACAAATCGGCGAGGAAACCTTAATTATCGCCGTATGCGCGATCATCAGCGGCTTAATTATCGGTTATTTACTGGCGCAAGTACTCGGCTTAA‑CCGTCTTCAAAGc                                                                                                                                                   <  1:800947/251‑1 (MQ=255)                                                                                         ggCTTGATGGGCTTGATTTCCGTGGTGATTCTGATTTTAGCCACCCTCTGCGTGAACACCACTCTCATCGCCATCGTGGGCGAACGGGCAAAAGAATTCGCGTTGCAAAAAGCCCTCGGCGCGAAAAGCCGGGACATCATCAAACAAATCGGCGAGGAAACCTTAATTATCGCCGTATGCGCGATCATCAGCGGCTTAATTATCGGTTATTTACTGGCGCAAGTACTCGGCTTAA‑CCGTCTTCAAAGCCTa                                                                                                                                                >  1:641151/1‑251 (MQ=255)                                                                                                  ggCTTGATTTCCGTGGTGATTCTGATTTTAGCCACCCTCTGCGTGAACACCACTCTCATCGCCATCGTGGGCGAACGGGCAAAAGAATTCGCGTTGCAAAAAGCCCTCGGCGCGAAAAGCCGGGACATCATCAAACAAATCGGCGAGGAAACCTTAATTATCGCCGTATGCGCGATCATCAGCGGCTTAATTATCGGTTATTTACTGGCGCAAGTACTCGGCTTAA‑CCGTCTTCAAAGCCTACATCGACAt                                                                                                                                       >  1:101228/1‑251 (MQ=255)                                                                                                           tCCGTGGTGATTCTGATTTTAGCCACCCTCTGCGTGAACACCACTCTCATCGCCATCGTGGGCGAACGGGCAAAAGAATTCGCGTTGCAAAAAGCCCTCGGCGCGAAAAGCCGGGACATCATCAAACAAATCGGCGAGGAAACCTTAATTATCGCCGTATGCGCGATCATCAGCGGCTTAATTATCGGTTATTTACTGGCGCAAGTACTCGGCTTAA‑CCGTCTTCAAAGCCTACATCGACa                                                                                                                                        <  1:21096/241‑1 (MQ=255)                                                                                                                       ctaaTTTTAGCCACCCTCTGCGTGAACACCACTCTCATCGCCATCGTGGGCGAACCGGCAAAAGAATTCGCGTTGCAAAAAGCCCTCGGCGCGAAAAGCCGGGACATCATCAAACAAATCGGCGAGGAAACCTTAATTATCGCCATATGCGCGATCATCAGCGGCTTAATTATCGGTTATTTACTGGCGCAAGTACTCGGCTTAA‑CCGTCTTCAAAGCCTACATCGACATGCGCCTGCCGGTGTTGCCGAt                                                                                                                  <  1:517909/248‑1 (MQ=255)                                                                                                                              tAGCCACCCTCTGCGTGAACACCACTCTCATCGCCATCGTGGGCGAACGGGCAAAAGAATTCGCGTTGCAAAAAGCCCTCGGCGCGAAAAGCCGGGACATCATCAAACAAATCGGCGAGGAAACCTTAATTATCGCCGTATGCGCGATCATCAGc                                                                                                                                                                                                            <  1:865653/155‑1 (MQ=255)                                                                                                                              tAGCCACCCTCTGCGTGAACACCACTCTCATCGCCATCGTGGGCGAACGGGCAAAAGAATTCGCGTTGCAAAAAGCCCTCGGCGCGAAAAGCCGGGACATCATCAAACAAATCGGCGAGGAAACCTTAATTATCGCCGTATGCGCGATCATCAGCGGCTTAATTATCGGTTATTTACTGGCGCAAGTACTCGGCTTAA‑CCGTCTTCAAAg                                                                                                                                                    <  1:552615/210‑1 (MQ=255)                                                                                                                              tAGCCACCCTCTGCGTGAACACCACTCTCATCGCCATCGTGGGCGAACGGGCAAAAGAATTCGCGTTGCAAAAAGCCCTCGGCGCGAAAAGCCGGGACATCATCAAACAAATCGGCGAGGAAACCTTAATTATCGCCGTATGCGCGATCATCAGCGGCTTAATTATCGGTTATTTACTGGCGCAAGTACTCGGCTTAA‑CCGTCTTCAAAGCCTACATCGACATGCGCCTGCCGGTGTTGCCGATCACCAt                                                                                                            >  1:731738/1‑250 (MQ=255)                                                                                                                                gCCACCCTCTGCGTGAACACCACTCTCATCGCCATCGTGGGCGAACGGGCAAAAGAATTCGCGTTGCAAAAAGCCCTCGGCGCGAAAAGCCGGGACATCATCAAACAAATCGGCGAGGAAACCTTAATTATCGCCGTATGCGCGATCATCAGCGGCTTAATTATCGGTTATTTACTGGCGCAAGTACTCGGCTTAA‑CCGTCTTCAAAGCCTACATCGACATGCGCCTGcc                                                                                                                              >  1:796771/1‑230 (MQ=255)                                                                                                                                  cACCCTCTGCGTGAACACCACTCTCATCGCCATCGTGGGCGAACGGGCAAAAGAATTCGCGTTGCAAAAAGCCCTCGGCGCGAAAAGCCGGGACATCATCAAACAAATCGGCGAGGAAACCTTAATTATCGCCGTATGCGCGATCATCAGCGGCTTAATTATCGGTTATTTACTGGCGCAAGTACTCGGCTTAA‑CCGTCTTCAAAGCCTACATCGACATGCGCCTGCCGGTGTTGCCGATCACCATCGGAt                                                                                                       <  1:141199/251‑1 (MQ=255)                                                                                                                                    cccTCTGCGTGAACACCACTCTCATCGCCATCGTGGGCGAACGGGCAAAAGAATTCGCGTTGCAAAAAGCCTTCGGCGCGAAAAGCCGGGACATCATCAAACAAATCGGCGAGGAAACCTTAATTATCGCCGTATGCGCGATCATCAGCGGCTTAATTATCGGTTATTTACTGGCGCAAGTACTCGGCTTAA‑CCGTCTTCAAAGCCTACATCGACATGCGCCTGCCGGTGTTGCCGATCAc                                                                                                               <  1:427474/241‑1 (MQ=255)                                                                                                                                    cccTCTGCGTGAACACCACTCTCATCGCCATCGTGGGCGAACGGGCAAAAGAATTCGCGTTGCAAAAAGCCCTCGGCGCGAAAAGCCGGGACATCATCAAACAAATCGGCGAGGAAACCTTAATTATCGCCGTATGCGCGATCATCAGCGGCTTAATTATCGGTTATTTACTGGCGCAAGTACTCGGCTTAA‑CCGTCTTCAAAGCCTACAt                                                                                                                                             <  1:850458/211‑1 (MQ=255)                                                                                                                                    cccTCTGCGTGAACACCACTCTCATCGCCATCGTGGGCGAACGGGCAAAAGAATTCGCGTTGCAAAAAGCCCTCGGCGCGAAAAGCCGGGACATCATCAAACAAATCGGCGAGGAAACCTTAATTATCGCCGTATGCGCGATCATCAGCGGCTTAATTATCGGTTATTTACTGGCGCAAGTACTCGGCTTAA‑CCGTCTTCAAAGCCTACATCGACATGCGCCTGCCGGTGTTGCCGATCACCATCGGATTa                                                                                                     >  1:744638/1‑251 (MQ=255)                                                                                                                                    cccTCTGCGTGAACACCACTCTCATCGCCATCGTGGGCGAACGGGCAAAAGAATTCGCGTTGCAAAAAGCCCTCGGCGCGAAAAGCCGGGACATCATCAAACAAATCGGCGAGGAAACCTTAATTATCGCCGTATGCGCGATCATCAGCGGCTTAATTATCGGTTATTTACTGGCGCAAGTACTCGGCTTAA‑CCGTCTTCAAAGCCTACATCGACATGCGCCTGCCGGTGTTGCCGATCACCATCGGATTa                                                                                                     >  1:399837/1‑251 (MQ=255)                                                                                                                                        ctGCGTGAACACCACTCTCATCGCCATCGTGGGCGAACGGGCAAAAGAATTCGCGTTGCAAAAAGCCCTCGGCGCGAAAAGCCGGGACATCATCAAACAAATCGGCGAGGAAACCTTAATTATCGCCGTATGCGCGATCATCAGCGGCTTAATTATCGGTTATTTACTGGCGCAAGTACTCGGCTTAA‑CCGTCTTCAAAGCCTACATCGACATGCGCCTGCCGGTGTTGCCGATCACCATCGGATTATc                                                                                                   >  1:34552/1‑249 (MQ=255)                                                                                                                                          gCGTGAACACCACTCTCATCGCCATCGTGGGCGAACGGGCAAAAGAATTCGCGTTGCAAAAAGCCCTCGGCGCGAAAAGCCGGGACATCATCAAACAAATCGGCGAGGAAACCTTAATTATCGCCGTATGCGCGATCATCAGCGGCTTAATTATCGGTTATTTACTGGCGCAAGTACTCGGCTTAA‑CCGTCTTCAAAGCCTACATCGACATGCGCCTGCCGGTGTTGCCGATCAc                                                                                                               <  1:494791/235‑1 (MQ=255)                                                                                                                                          gCGTGAACACCACTCTCATCGCCATCGTGGGCGAACGGGCAAAAGAATTCGCGTTGCAAAAAGCCCTCGGCGCGAAAAGCCGGGACATCATCAAACAAATCGGCGAGGAAACCTTAATTATCGCCGTATGCGCGATCATCAGCGGCTTAATTATCGGTTATTTACTGGCGCAAGTACTCGGCTTAA‑CCGTCTTCAAAGCCTACATCGACATGCGCCTGCCGGTGTTGCCGATCACCATCGGATTATCCtta                                                                                               >  1:256048/1‑251 (MQ=255)                                                                                                                                             tGAACACCACTCTCATCGCCATCGTGGGCGAACGGGCAAAAGAATTCGCGTTGCAAAAAGCCCTCGGCGCGAAAAGCCGGGACATCATCAAACAAATCGGCGAGGAAACCTTAATTATCGCCGTATGCGCGATCATCAGCGGCTTAATTATCGGTTATTTACTGGCGCAAGTACTCGGCTTAA‑CCGTCTTCAAAGCCTACATCGACATGCGCCTGCCGGTGTTGCCGATCACCATCGGATTATCCttatt                                                                                             <  1:323729/250‑1 (MQ=255)                                                                                                                                               aaCACCACTCTCATCGCCATCGTGGGCGAACGGGCAAAAGAATTCGCGTTGCAAAAAGCCCTCGGCGCGAAAAGCCGGGACATCATCAAACAAATCGGCGAGGAAACCTTAATTATCGCCGTATGCGCGATCATCAGCGGCTTAATTATCGGTTATTTACTGGCGCAAGTACTCGGCTTAA‑CCGTCTTCAAAGCCTACATCGACATg                                                                                                                                      >  1:419546/1‑207 (MQ=255)                                                                                                                                                acacCACTCTCATCGCCATCGTGGGCGAACGGGCAAAAGAATTCGCGTTGCAAAAAGCCCTCGGCGCGAAAAGCCGGGACATCATCAAACAAATCGGCGAGGAAACCTTAATTATCGCCGTATGCGCGATCATCAGCGGCTTAATTATCGGTTATTTACTGGCGCAAGTACTCGGCTTAA‑CCGTCTTCAAAGCCTACATCGACATGCGCCTGCCGGTGTTGCCGATCACCATCGGATTATCCttattaatg                                                                                         >  1:828466/1‑248 (MQ=255)                                                                                                                                                 caccacTCTCATCGCCATCGTGGGCGAACGGGCAAAAGAATTCGCGTTGCAAAAAGCCCTCGGCGCGAAAAGCCGGGACATCATCAAACAAATCGGCGAGGAAACCTTAATTATCGCCGTATGcgcg                                                                                                                                                                                                                     <  1:315773/127‑1 (MQ=255)                                                                                                                                                   ccacTCTCATCGCCATCGTGGGCGAACGGGCAAAAGAATTCGCGTTGCAAAAAGCCCTCGGCGCGAAAAGCCGGGACATCATCAAACAAATCGGCGAGGAAACCTTAATTATCGCCGTATGCGCGATCATCAGCGGCTTAATTATCGGTTATTTACTGGCGCAAGTACTCGGCTTAA‑CCGTCTTCAAAGCCTACATCGACATGCGCCTGCCGGTGTTGCCGATCACCATCGGATTATCCttatt                                                                                             <  1:70652/244‑1 (MQ=255)                                                                                                                                                     acTCTCATCGCCATCGTGGGCGAACGGGCAAAAGAATTCGCGTTGCAAAAAGCCCTCGGCGCGAAAAGCCGGGACATCATCAAACAAATCGGCGAGGAAACCTTAATTATCGCCGTATGCGCGATCATCAGCGGCTTAATTATCGGTTATTTACTGGCGCAAGTACTCGGCTTAA‑CCGTCTTCAAAGCCTACATCGACATGCGCCTGCCGGTGTTGCCGATCACCATCGGATTATCCTTATTAATGGCGtt                                                                                    >  1:391954/1‑251 (MQ=255)                                                                                                                                                     acTCTCATCGCCATCGTGGGCGAACGGGCAAAAGAATTCGCGTTGCAAAAAGCCCTCGGCGCGAAAAGCCGGGACATCATCAAACAAATCGGCGAGGAAACCTTAATTATCGCCGTATGCGCGATCATCAGCGGCTTAATTATCGGTTATTTACTGGCGCAAGTACTCGGCTTAA‑CCGTCTTCAAAGCCTACATCGACATGCGCCTGCCGGTGTTGCCGATCACCATCGGATTATCCTTATTAATGGCGtt                                                                                    >  1:664460/1‑251 (MQ=255)                                                                                                                                                      ctctCATCGCCATCGTGGGCGAACGGGCAAAAGAATTCGCGTTGCAAAAAGCCCTCGGCGCGAAAAGCCGGGACATCATCAAACAAATCGGCGAGGAAACCTTAATTATCGCCGTATGCGCGATCATCAGCGGCTTAATTATCGGTTATTTACTGGCGCAAGTACTCGGCTTAA‑CCGTCTTCAAAGCCTACATCGACATGCGCCTGCCGGTGTTGCCGATCACCATCGGATTATCCTTATTAATGGCGttt                                                                                   >  1:815354/1‑251 (MQ=255)                                                                                                                                                        ctcATCGCCATCGTGGGCGAACGGGCAAAAGAATTCGCGTTGCAAAAAGCCCTCGGCGCGAAAAGCCGGGACATCATCAAACAAATCGGCGAGGAAACCTTAATTATCGCCGTa                                                                                                                                                                                                                           >  1:583236/1‑114 (MQ=255)                                                                                                                                                        ctcATCGCCATCGTGGGCGAACGGGCAAAAGAATTCGCGTTGCAAAAAGCCCTCGGCGCGAAAAGCCGGGACATCATCAAACAAATCGGCGAGGAAACCTTAATTATCGCCGTATGCGCGATCATCAGCGGCTTAATTATCGGTTATTTACTGGCGCAAGTACTCGGCTTAA‑CCGTCTTCAAAGCCTACATc                                                                                                                                            <  1:561751/192‑1 (MQ=255)                                                                                                                                                          cATCGCCATCGTGGGCGAACGGGCAAAAGAATTCGCGTTGCAAAAAGCCCTCGGCGCGAAAAGCCGGGACATCATCAAACAAATCGGCGAGGAAACCTTAATTATCGCCGTATGCGCGATCATCAGCGGCTTAATTATCGGTTATTTACTGGCGCAAGTACTCGGCTTAA‑CCGTCTTCAAAGCCTACATCGACATGCGCCTGCCGGTGTTGCCGATCACCATCGGATTATCCTTATTAATGGCGTTTATCg                                                                               >  1:899857/1‑251 (MQ=255)                                                                                                                                                            tCGCCATCGTGGGCGAACGGGCAAAAGAATTCGCGTTGCAAAAAGCCCTCGGCGCGAAAAGCCGGGACATCATCAAACAAATCGGCGAGGAAACCTTAATTATCGCCGTATGCGCGATCATCAGCGGCTTAATTATCGGTTATTTACTGGCGCAAGTACTCGGCTTAA‑CCGTCTTCAAAGCCTACATCGACATGCGCCTGCCGGTGTTGCCGATc                                                                                                                 <  1:315991/215‑1 (MQ=255)                                                                                                                                                                       ggCGAACGGGCAAAAGAATTCGCGTTGCAAAAAGCCCTCGGCGCGAAAAGCCGGGACATCATCAAACAAATCGGCGAGGAAACCTTAATTATCGCCGTATGCGCGATCATCAGCGGCTTAATTATCGGTTATTTACTGGCGCAAGTACTCGGCTTAA‑CCGTCTTCAAAGCCTACATCGACATGCGCCTGCCGGTGTTGCCGATCACCATCGGATTATCCTTATTAATGGCGTTTATCGCAGTTATCGTAc                                                                   <  1:524989/250‑1 (MQ=255)                                                                                                                                                                           aaCGGGCAAAAGAATTCGCGTTGCAAAAAGCCCTCGGCGCGAAAAGCCGGGACATCATCAAACAAATCGGCGAGGAAACCTTAATTATCGCCGTATGCGCGATCATCAGCGGCTTAATTATCGGTTATTTACTGGCGCAAGTACTCGGCTTAA‑CCGTCTTCAAAGCCTACATCGACATGCGCCTGCCGGTGTTGCCGATCACCATCGGATTATCCTTATTAATGGCGTTTATCGCAGTTATCGTACCGAcc                                                              <  1:577338/251‑1 (MQ=255)                                                                                                                                                                             cGGGCAAAAGAATTCGCGTTGCAAAAAGCCCTCGGCGCGAAAAGCCGGGACATCATCAAACAAATCGGCGAGGAAACCTTAATTATCGCCGTATGCGCGATCATCAGCGGCTTAATTATCGGTTATTTACTGGCGCAAGTACTCGGCTTAA‑CCGTCTTCAAAGCCTACATCGACATGCGCCTGCCGGTGTTGCCGATCACCATCGGATTATCCTTATTAATGGCGTTTATCGCAGTTATCGTACCGACccg                                                            >  1:392131/1‑251 (MQ=255)                                                                                                                                                                               ggCAAAAGAATTCGCGTTGCAAAAAGCCCTCGGCGCGAAAAGCCGGGACATCATCAAACAAATCGGCGAGGAAACCTTAATTATCGCCGTATGCGCGATCATCAGCGGCTTAATTATCGGTTATTTACTGGCGCAAGTACTCGGCTTAA‑CCGTCTTCAAAGCCTACATCGACATGCGCCTGCCGGTGTTGCCGATCACCATCGGATTATCCTTATTAATGGCGTTTATCGCAGTTATCGTACCGACccgc                                                           >  1:296809/1‑250 (MQ=255)                                                                                                                                                                                  aaaaGAATTCGCGTTGCAAAAAGCCCTCGGCGCGAAAAGCCGGGACATCATCAAACAAATCGGCGAGGAAACCTTAATTATCGCCGTATGCGCGATCATCAGCGGCTTAATTATCGGTTATTTACTGGCGCAAGTACTCGGCTTAA‑CCGTCTTCAAAGCCTACATCGACATGCGCCTGCCGGTGTTGCCGATCACCATCGGATTATCCTTATTAATGGCGTTTATCGCAGTTATCGTACCGACCCGCcgcg                                                       >  1:25242/1‑251 (MQ=255)                                                                                                                                                                                                  cAAAAAGCCCTCGGCGCGAAAAGCCGGGACATCATCAAACAAATCGGCGAGGAAACCTTAATTATCGCCGTATGCGCGATCATCAGCGGCTTAATTATCGGTTATTTACTGGCGCAAGTCCTCGGCTTAA‑CCGTCTTCAAAGCCTACATCGACATGCGCCTGCCGGTGTTGCCGATCACCATCGGATTATCCTTATTAATGGCGTTTATCGCAGTTATCGTACCGACCCGCCGCGCCTTAGACATTCAAAc                                       <  1:456566/251‑1 (MQ=255)                                                                                                                                                                                                            ccGGCGCAAAAACCCGGGACATCATCAAACAAATCGGCGAGGAACCCTTAATTATCGCCGTAGGCGCGATCATCAGCGGTTTAATTATCGTTTATTTCCTGGCGCAAGTACTCGGCTTAA‑CCGTCTTCAAAGCCTACATCGACATGCGCCTGCCGGTGTTGCCGATCCCCATCGGATTATCCTTATTAAGGGCTTTTATCGCAGTTATCGTACCGACCCGCCGCGCCTTAGACATTCAAACCGCCAAtgtg                             <  1:596508/250‑1 (MQ=255)                                                                                                                                                                                                                        gCCGGGACATCATCAAACAAATCGGCGAGGAAACCTTAATTATCGCCGTATGCGCGATCATCAGCGGCTTAATTATCGGTTATTTACTGGCGCAAGTACTCGGCTTAA‑CCGTCTTCAAAGCCTACATCGACATGCGCCTGCCGGTGTTGCCGATCACCATCGGATTATcc                                                                                                  <  1:699652/170‑1 (MQ=255)                                                                                                                                                                                                                              aCATCATCAAACAAATCGGCGAGGAACCCTTAATTATCGCCGTAGGCGCGATCATCAGCGGCTTAATTATCGGTTATTTCCGGGCGCAAGTACTCGGTTAAA‑CCGTCTTAAAAGCCTACTTCGACAGGCGCCTGCCGGTGTTGCCGACCCCCACCGGATTATCCTTATTAATGGCTTTTATCGCATTTATCGTACCGCCCCGCCGCGCCTTAGACATTCAAACCGCCAATGTGTTAAAAGGGGAATAACAt           <  1:689238/251‑1 (MQ=255)                                                                                                                                                                                                                                 tcatcaAACAAATCGGCGAGGAAACCTTAATTATCGCCGTATGCGCGATCATCAGCGGCTTAATTATCGGTTATTTACTGGCGCAAGTGCTCGGCTTAA‑CCGTCTTCAAAGCCTACATCGACATGCGCCTGCCGGTGTTGCCGATCACCATCGGATTATCCttatt                                                                                             <  1:11935/166‑1 (MQ=255)                                                                                                                                                                                                                                 tcatcaAACAAATCGGCGAGGAAACCTTAATTATCGCCGTATGCGCGATCATCAGCGGCTTAATTATCGGTTATTTACTGGCGCAAGTACTCGGCTTAA‑CCGTCTTCAAAGCCTACATCGACATGCGCCTGCCGGTGTTGCCGATCACCATCGGATTATcc                                                                                                  <  1:360586/161‑1 (MQ=255)                                                                                                                                                                                                                                 tcatcaAACAAATCGGCGAGGAAACCTTAATTATCGCCGTATGCGCGATCATCAGCGGCTTAATTATCGGTTATTTACTGGCGCAAGTACTCGGCTTAA‑CCGTCTTCAAAGCCTACATCGACATGCGCCTGCCGGTGTTGCCGATCACCATCGGATTATCCTTATTAATGGCGTTTATCGCAGTTATCGTACCGACCCGCCGCGCCTTAGACATTCAAACCGCCAATGTGTTAAAAGGGGAATAACATgg         >  1:472284/1‑250 (MQ=255)                                                                                                                                                                                                                                 tcatcaAACAAATCGGCGAGGAAACCTTAATTATCGCCGTATGCGCGATCATCAGCGGCTTAATTATCGGTTATTTACTGGCGCAAGTACTCGGCTTAA‑CCGTCTTCAAAGCCTACATCGACATGCGCCTGCCGGTGTTGCCGATCACCATCGGATTATCCTTATTAATGGCGTTTATCGCAGTTATCGTACCGACCCGCCGCGCCTTAGACATTCAAACCGCCAATGTGTTAAAAGGGGAATAACATgg         >  1:482525/1‑250 (MQ=255)                                                                                                                                                                                                                                     caaacaaaTCGGCGAGGAAACCTTAATTATCGCCGTATGCGCGATCATCAGCGGCTTAATTATCGGTTATTTACTGGCGCAAGTACTCGGCTTAA‑CCGTCTTCAAAGCCTACATCGACATGCGCCTGCCGGTGTTGCCGATCACCATCGGATTATCCTTATTAATGGCGTTTATCGCAGTTATCGTACCGACCCGCCGCGCCTTAGACATTCAAACCGCCAATGTGTTAAAAGGGGAATAACATGGCTaaa    >  1:588043/1‑251 (MQ=255)                                                                                                                                                                                                                                       aacaaaTCGGCGAGGAACCCTAATTTACCGCCGTATGCGCGATCACCAGCGGTTTAATTATCGTTTATTTACGGGCGCAGGTCCT‑GGGTTAACCCGTCTCCAAACCCTACACCGACATGCGCCTGCGGGTGTTGCCGACCCCCATCGGTTTATCCTTATTAAGGGCTTTTATCGCAGTTATCGTCCCGACCCGCCGCGCCTTAGACATTCAAACCGCCAATGTGTTAAAAGGGGAATAACATGGCTAAAAc  <  1:11922/251‑1 (MQ=255)                                                                                                                                                                                                                                       aacaaaTCGGCGAGGAAACCTTAATTATCGCCGTATGCGCGTTCATCAGCGGTTTAATTATCGGTTATTTCCGGGCGCAAGTACTCGGCTTAA‑CCGTCTTAAAAGCCTACATCGACATGCCCCTGCCGTTGTTGCCGATCCCCATCGGATTATCCTTATTAATGGCTTTTATCGCAGTTATCGTACCGACCCGCCGCGCCTTAGACATTCAAACCGCCAATGTGTTAAAAGGGGAATAACATGGCTAAAAc  <  1:159033/251‑1 (MQ=255)                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                GCAACTTCAGCAACAATATCCCGATTTGGATATTCATCCGATCCGCAAAGTCTCCGCCTCCGAAGGGCAAATTCTGGATAAGATTAAAGGCTTGATGGGCTTGATTTCCGTGGTGATTCTGATTTTAGCCACCCTCTGCGTGAACACCACTCTCATCGCCATCGTGGGCGAACGGGCAAAAGAATTCGCGTTGCAAAAAGCCCTCGGCGCGAAAAGCCGGGACATCATCAAACAAATCGGCGCGGAAACCTTAATTATCGCCGTATGCGCGATCATCAGCGGCTTAATTATCGGTTATTTACTGGCGCAAGTACTCGGCTTAA‑CCGTCTTCAAAGCCTACATCGACATGCGCCTGCCGGTGTTGCCGATCACCATCGGATTATCCTTATTAGTGGCGTTTATCGCAGTTATCGTACCGACCCGCCGCGCCTTAGACATTCAAACCGCCAATGTGTTAAAAGGGGAATAACATGGCTAAAAC  >  1/1685588‑1686068

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑