Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	26,914	T→G	intergenic (–/+104220)	– / ← unknown	–/tRNA‑Ala‑GGC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	26,914	0	T	G	100.0%	170.6 / NA	63	intergenic (–/+104220)	–/unknown	–/tRNA‑Ala‑GGC
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (33/30);  total (33/30)

TGGCGTTGCGCTGCTCATATTCATAAGGGCAACCAGTGTCCCACGCAGGCGCCGGTGCCGAAACGTACACTACCGACCATAGAATTTGCCGAGCAGGCGGCATTCGGGAATTTTTCTAAAATGGACGCCATGGCGTTGGAAGAAATCAGCGAGTTTTGTCCCACAAAAATCGCAATGCCAATTGCAATTGCCCAAAAGCCCAAATTCAGCAACAGCACCAGAACCAACCAAATTTCCGCCAAAAACTGAACCATCAAACCAACGCGCAACATAGTTTCCGCGCCGACCTTATGCACCAATCGCCCATTCAGGAAGGAACCAAAGGTCATCACCGAGATATTCAGCATAAAGAAATAACCGAAATTCTCCGGCTTAATGCCGTAAATGCCGATATACACAATAGACCCCGCCGTGATGAAAGAAAATAAGCCCCCGAAAGCAAAAGAAGCGGCGAACATATACCCCAGCAC  >  1/26680‑27149                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                            ttttgtttcgctttttttttgcgatttttaaaacttttcgcattttttattcttgtctattcttaaataaaaaaggacgccgttttttcttgcgcccttgttttcattggttttgttccacttattcccgcttgattcaacattctccctagtttattcctttttctcattttgttcggtgttttacaTGCCCAAAAGCCCAAATTCAGCAACAGCACCAGAACCAACCAAATTGCCGCCAAAAACTGAAc                                                                                                                                                                                                                             >  1:788025‑M1/189‑251 (MQ=255)     gtttcgctttttttttgcgatttttaaaacttttcgcattttttattcttgtctattcttaaataaaaaaggacgccgttttttcttgcgcccttgttttcattggttttgttccacttattcccgcttgattcaacattctccctagtttattcctttttctcattttattcggtgttttacaTGCCCAAAAGCCCAAATTCAGCAACAGCACCAGAACCAACCAAATTGCCGCCAAAAACTGAACCAt                                                                                                                                                                                                                          <  1:446769‑M1/66‑1 (MQ=255)                         tttttaaaacttttcgcattttttattcttgtctattcttaaataaaaaaggacgccgttttttcttgcgcccttgttttcattggttttgttccacttattcccgcttgattcaacattctccctagtttattcctttttctcattttgttcggtgttttacaTGCCCGAAAGCCCAAATTCAGCAACAGCACCAGAACCAACCAAATTGCCGCCAAAAACTGAACCATCAAACCAACGCGCAACATAGt                                                                                                                                                                                                     <  1:444571‑M1/87‑1 (MQ=255)                                           ttttttattcttgtctattcttaaataaaaaaggacgccgttttttcttgcgcccttgttttcattggttttgttccacttattcccgcttgattcaacattctccctagtttattcctttttctcattttgttcggtgttttacaTGCCCAAAAGCCCAAATTCAGCAACAGCACCAGAACCAACCAAATTGCCGCCAAAAACTGAACCATCAAACCAACGCGCAACATAGTTTCCGCGCCGACCTTATg                                                                                                                                                                                   <  1:320532‑M1/105‑1 (MQ=255)                                           ttttttattcttgtctattcttaaataaaaaaggacgccgttttttcttgcgcccttgttttcattggttttgttccacttattcccgcttgattcaacattctccctagtttattcctttttctcattttgttcggtgttttacaTGCCCAAAAGCCCAAATTCAGCAACAGCACCAGAACCAACCAAATTGCCGCCAAAAACTGAACCATCAAACCAACGCGCAACATAGTTTCCGCGCCGACCTTATg                                                                                                                                                                                   <  1:19564‑M1/105‑1 (MQ=255)                                           ttttttattcttgtctattcttaaataaaaaaggacgccgttttttcttgcgcccttgttttcattggttttgttccacttattcccgcttgattcaacattctccctagtttattcctttttctcattttgttcggtgttttacaTGCCCAAAAGCCCAAATTCAGCAACAGCACCAGAACCAACCAAATTGCCGCCAAAAACTGAACCATCAAACCAACGCGCAACATAGTTTCCGCGCCGACCTTATg                                                                                                                                                                                   <  1:677010‑M1/105‑1 (MQ=255)                                              tttattcttgtctattcttaaataaaaaaggacgccgttttttcttgcgcccttgttttcattggttttgttccacttattcccgcttgattcaacattctccctagtttattcctttttctcattttgttcggtgttttacaTGCCCAAAAGCCCAAATTCAGCAACAGCACCAGAACCAACCAAATTGCCGCCAAAAACTGAACCATCAAACCAACGCGCAACATAGTTTCCGCGCCGACCTTATGCAc                                                                                                                                                                                >  1:601919‑M1/144‑251 (MQ=255)                                                 attcttgtctattcttaaataaaaaaggacgccgttttttcttgcgcccttgttttcattggttttgttctacttattcccgcttgattcaacattctccctagtttattcctttttctcattttgttcggtgttttacaTGCCCAAAAGCCCAAATTCAGCAACAGCACCAGAACCAACCAAATTGCCGCCAAAAACTGAACCATCAAACCAACGCGCAACATAGTTTCCGCGCCGAcc                                                                                                                                                                                        >  1:451625‑M1/141‑240 (MQ=255)                                                       gtctattcttaaataaaaaaggacgccgttttttcttgcgcccttgttttcattggttttgttccacttattcccgcttgattcaacattctccctagtttattcctttttctcattttgttcggtgttttacaTGCCCAAAAGCCCAAATTCAGCAACAGCACCAGAACCAACCAAATTGCCGCCAAAAACTGAACCATCAAACCAACGCGCAACATAGTTTCCGCGCCGACCTTATGCACCAATCGcc                                                                                                                                                                        <  1:441102‑M1/116‑1 (MQ=255)                                                                taaataaaaaaggacgccgttttttcttgcgcccttgttttcattggttttgttccacttattcccgcttgattcaacattctccctagtttattcctttttctcattttgttcggtgttttacaTGCCCAAAAGCCCAAATTCAGCAACAGCACCAGAACCAACCAAATTGCCGCCAAAAACTGAACCATCAAACCAACGCGCAACATAGTTTCCGCGCCGACCTTATGCACCAATCGCCCATTCaggaa                                                                                                                                                              >  1:765031‑M1/126‑251 (MQ=255)                                                                            gacgccgttttttcttgcgcccttgttttcattggttttgttccacttattcccgcttgattcaacattctccctagtttattcctttttctcattttgttcggtgttttacaTGCCCAAAAGCCCAAATTCAGCAACAGCACCAGAACCAACCAAATTGCCGCCAAAAACTGAACCATCAAACCAACGCGCAACATAGTTTCCGCGCCGACCTTATGCCCCAATCGCCCATTCAGGAAGGAACCAAAgg                                                                                                                                                   >  1:335550‑M1/114‑250 (MQ=255)                                                                            gacgccgttttttcttgcgcccttgttttcattggttttgttccacttattcccgcttgattcaacattctccctagtttattcctttttctcattttgttcggtgttttacaTGCCCAAAAGCCCAAATTCAGCAACAGCACCAGAACCAACCAAATTGCCGCCAAAAACTGAACCATCAAACCAACGCGCAACATAGTTTCCGCGCCGACCTTATGCACCAATCGCCCATTCAGGAAGGAACCAAAgg                                                                                                                                                   >  1:832489‑M1/114‑250 (MQ=255)                                                                            gacgccgttttttcttgcgcccttgttttcattggttttgttccacttattcccgcttgattcaacattctccctagtttattcctttttctcattttgttcggtgttttacaTGCCCAAAAGCCCAAATTCAGCAACAGCACCAGAACCAACCAAATTGCCGCCAAAAACTGAACCATCAAACCAACGCGCAACATAGTTTCCGCGCCGACCTTATGCACCAATCGCCCATTCAGGAAGGAACCAAAgg                                                                                                                                                   >  1:212152‑M1/114‑250 (MQ=255)                                                                            gacgccgttttttcttgcgcccttgttttcattggttttgttccacttattcccgcttgattcaacattctccctagtttattcctttttctcattttgttcggtgttttacaTGCCCAAAAGCCCAAATTCAGCAACAGCACCAGAACCAACCAAATTGCCGCCAAAAACTGAACCATCAAACCAACGCGCAACATAGTTTCCGCGCCGACCTTATGCACCAATCGCCCATTCAGGAAGGAACCAAAg                                                                                                                                                    >  1:147901‑M1/114‑249 (MQ=255)                                                                             acgccgttttttcttgcgcccttgttttcattggttttgttccacttattcccgcttgattcaacattctccctagtttattcctttttctcattttgttcggtgttttacaTGCCCAAAAGCCCAAATTCAGCAACAGCACCAGAACCAACCAAATTGCCGCCAAAAACTGAACCATCAAACCAACGCGCAACATAGTTTCCGCGCCGACCTTATGCACCAATCGCCCATTCAGGAAGGAACCAAAGGt                                                                                                                                                  >  1:167400‑M1/113‑250 (MQ=255)                                                                                ccgttttttcttgcgcccttgttttcattggttttgttccacttattcccgcttgattcaacattctccctagtttattcctttttctcattttgttcggtgttttacaTGCCCAAAAGCCCAAATTCAGCAACAGCACCAGAACCAACCAAATTGCCGCCAAAAACTGAACCATCAAACCAACGCGCAACATAGtt                                                                                                                                                                                                    >  1:297207‑M1/110‑197 (MQ=255)                                                                                  gttttttcttgcgcccttgttttcattggttttgttccacttattcccgcttgattcaacattctccctagtttattcctttttctcattttgttcggtgttttacaTGCCCAAAAGCCCAAATTCAGCAACAGCACCAGAACCAACCAAATTGCCGCCAAAAACTGAACCATCAAACCAACGCGCAACATAGTTTCCGCGCCGACCTTATGCACCAATCGCCCATTCAGGAAGGAACCAAAGGtcatca                                                                                                                                             <  1:725008‑M1/143‑1 (MQ=255)                                                                                       ttcttgcgcccttgttttcattggttttgttccacttattcccgcttgattcaacattctccctagtttattcctttttctcattttgttcggtgttttacaTGCCCAAAAGCCCAAATTCAGCAACAGCACCAGAACCAACCAAATTGCCGCCAAAAACTGAACCATCAAACCAACGCGCAACATAGTTTCCGCGCCGACCTTATGCACCAATCGCCCATTCAGGAAGGAACCAAAGGTCATCACCgaga                                                                                                                                       >  1:677987‑M1/103‑251 (MQ=255)                                                                                               cccttgttttcattggttttgttccacttattcccgcttgattcaacattctccctagtttattcctttttctcattttgttcggtgttttacaTGCCCAAAAGCCCAAATTCAGCAACAGCACCAGAACCAACCAAATTGCCGCCAAAAACTGAACCATCAACCCACCCCCCAACATAGTTTCCGCGCCGCCTTTATGCCCCAACCGCCcattcaggagggaaccaaaggcctgccccttatacacatc                                                                                                                                <  1:196400‑M1/156‑20 (MQ=255)                                                                                               cccttgttttcattggttttgttccacttattcccgcttgattcaacattctccctagtttattcctttttctcattttgttcggtgttttacaTGCCCAAAAGCCCAAATTCAGCAACAGCACCAGAACCAACCAAATTGCCGCCAAAAACTGAACCATCAAACCAACGCGCAACATAGTTTc                                                                                                                                                                                                  >  1:159427‑M1/95‑184 (MQ=255)                                                                                                 cttgttttcattggttttgttccacttattcccgcttgattcaacattctccctagtttattcctttttctcattttgttcggtgttttacaTGCCCAAAAGCCCAAATTCAGCAACAGCACCAGAACCAACCAAATTGCCGCCAAAAACTGAACCATCAAACCAACGCGCAACATAGTTTCCGCGCCGACCTTATGCACCAATCGCCCATTCAGGAAGGAACCAAAGGTCATCACCGAGATATTCAGCAt                                                                                                                             >  1:240038‑M1/93‑251 (MQ=255)                                                                                                      tttcattggttttgttccacttattcccgcttgattcaacattctccctagtttattcctttttctcattttgttcggtgttttacaTGCCCAAAAGCCCAAATTCAGCAACAGCACCAGAACCAACCAAATTGCCGCCAAAAACTGAACCATCAAACCAACGCGCAACATAGTTTCCGCGCCGACCTTATGCACCAATCGCCCATTCAGGAAGGAACCAAAGGTCATCACCGAGATATTCAGCATAAAGa                                                                                                                        >  1:897654‑M1/88‑251 (MQ=255)                                                                                                      tttcattggttttgttccacttattcccgcttgattcaacattctccctagtttattcctttttctcattttgttcggtgttttacaTGCCCAAAAGCCCAAATTCAGCAACAGCACCAGAACCAACCAAATTGCCGCCAAAAACTGAACCATCAAACCAACGCGCAACATAGTTTCCGCGCCGACCTTATGCACCAATCGCCCATTCAGGAAGGAACCAAAGGTCATCACCGAGATATTCAGCATAAAGa                                                                                                                        >  1:220939‑M1/88‑251 (MQ=255)                                                                                                              gttttgttccacttattcccgcttgattcaacattctccctagtttattcctttttctcattttgttcggtgttttacaTGCCCAAAAGCCCAAATTCAGCAACAGCACCAGAACCAACCAAATTGCCGCCAAAAACTGAACCATCAAACCAACGCGCAACATAGTTTCCGCGCCGACCTTATGCACCAATCGCCCATTCAGGAAGGAACCAAAGGTCATCACCGAGATATTCAGCATAAAGAAATAAcc                                                                                                                 >  1:624182‑M1/80‑250 (MQ=255)                                                                                                              gttttgttccacttattcccgcttgattcaacattctccctagtttattcctttttctcattttgttcggtgttttacaTGCCCAAAAGCCCAAATTCAGCAACAGCACCAGAACCAACCAAATTGCCGCCAAAAACTGAACCATCAAACCAACGCGCAACATAGTTTCCGCGCCGACCTTATGCACCAATCGCCCATTCAGGAAGGAACCAAAGGTCATCACCGAGATATTCAGCATAAAGAAATAACCg                                                                                                                <  1:652583‑M1/172‑1 (MQ=255)                                                                                                                 ttgttccacttattcccgcttgattcaacattctccctagtttattcctttttctcattttgttcggtgttttacaTGCCCAAAAGCCCAAATTCAGCAACAGCACCAGAACCAACCAAATTGCCGCCAAAAACTGAACCATCAAACCAACGCGCAACATAGTTTCCGCGCCGACCTTATGCACCAATCGCCCATTCAGGAAGGAACCAAAGGTCATCACCGAGATATTCAGCATAAAGa                                                                                                                        <  1:690272‑M1/164‑1 (MQ=255)                                                                                                                        acttattcccgcttgattcaacattctccctagtttattcctttttctcattttgttcggtgttttacaTGCCCAAAAGCCCAAATTCAGCAACAGCACCAGAACCAACCAAATTGCCGCCAAAAACTGAACCATCAAACCAACGCGCAACATAGTTTCCGCGCCGACCTTATGCACCAATCGCCCATTCAGGAAGGAACCAAAgg                                                                                                                                                   <  1:484441‑M1/137‑1 (MQ=255)                                                                                                                         cttattcccgcttgattcaacattctccctagtttattcctttttctcattttgttcggtgttttacaTGCCCAAAAGCCCAAATTCAGCAACAGCACCAGAACCAACCAAATTGCCGCCAAAAACTGAACCATCAAACCAACGCGCAACATAGTTTCCGCGCCGACCTTATGCACCAATCGCCCATTCAGGAAGGAACCAAAGGTCATCACCgaga                                                                                                                                       >  1:741566‑M1/69‑217 (MQ=255)                                                                                                                         cttattcccgcttgattcaacattctccctagtttattcctttttctcattttgttcggtgttttacaTGCCCAAAAGCCCAAATTCAGCAACAGCACCAGAACCAACCAAATTGCCGCCAAAAACTGAACCATCAAACCAACGCGCAACATAGTTTCCGCGCCGACCTTATGCACCAATCGCCCATTCAGGAAGGAACCAAAGGTCATCACCGAGATATTCAGCATAAAGAAATAACCGAAATTCTCCgg                                                                                                     <  1:61844‑M1/183‑1 (MQ=255)                                                                                                                            attcccgcttgattcaacattctccctagtttattcctttttctcattttgttcggtgttttacaTGCCCAAAAGCCCAAATTCAGCAACAGCACCAGAACCAACCAAATTGCCGCCAAAAACTGAACCATCAAACCAACGCGCAACATAGTTTCCGCGCCGACCTTATGCACCAATCGCCCATTCAGGAAGGAACCAAAGGTCATCACCGAGATATTCAGCATAAAGAAATAACCGAAATTCTCCGGCtt                                                                                                  <  1:488163‑M1/186‑1 (MQ=255)                                                                                                                                   cttgattcaacattctccctagtttattcctttttctcattttgttcggtgttttacaTGCCCAAAAGCCCAAATTCAGCAACAGCACCAGAACCAACCAAATTGCCGCCAAAAACTGAACCATCAAACCAACGCGCAACATAGTTTCCGCGCCGACCTTATGCACCAATCGCCCATTCAGGAAGGAACCAAAgg                                                                                                                                                   <  1:875583‑M1/137‑1 (MQ=255)                                                                                                                                       attcaacattctccctagtttattcctttttctcattttgttcggtgttttacaTGCCCAAAAGCCCAAATTCAGCAACAGCACCAGAACCAACCAAATTGCCGCCAAAAACTGAACCATCAAACCAACGCGCAACATAGTTTCCGCGCCGACCTTATGCAc                                                                                                                                                                                >  1:735345‑M1/55‑162 (MQ=255)                                                                                                                                            acattctccctagtttattcctttttctcattttgttcggtgttttacaTGCCCAAAAGCCCAAATTCAGCAACAGCACCAGAACCAACCAAATTGCCGCCAAAAACTGAACCATCAAACCAACGCGCAACATAGTTTCCGCGCCGACCTTATGCACCAATCGCCCATTCAGGAAGGAACCAAAGGTCATCACCGAGATATTCAGCATAAAGAAATAACCGAAATTCTCCGGCTTAATGCCGTAAAtgcag                                                                                  <  1:789438‑M1/202‑3 (MQ=255)                                                                                                                                                tctccctagtttattcctttttctcattttgttcggtgttttacaTGCCCAAAAGCCCAAATTCAGCAACAGCACCAGAACCAACCAAATTGCCGCCAAAAACTGAACCATCAAACCAACGCGCAACATAGTTTCCGCGCCGACCTTATGCACCAATCGCCCATTCAGGCAGGAACCAAAGGTCATCACCGAGATATTCAGCATAAAGAAATAACCGAAATTCTCCGGCTTAATGCCGTAAATGCCGatat                                                                              <  1:453982‑M1/206‑1 (MQ=255)                                                                                                                                                   ccctagtttattcctttttctcattttgttcggtgttttacaTGCCCAAAAGCCCAAATTCAGCAACAGCACCAGAACCAACCAAATTGCCGCCAAAAACTGAACCATCAAACCAACGCGCAACATAGTTTCCGCGCCGACCTTATGc                                                                                                                                                                                  >  1:285213‑M1/43‑148 (MQ=255)                                                                                                                                                   ccctagtttattcctttttctcattttgttcggtgttttacaTGCCCAAAAGCCCAAATTCAGCAACAGCACCAGAACCAACCAAATTGCCGCCAAAAACTGAACCATCAAACCAACGCGCAACATAGTTTCCGCGCCGACCTTATGCACCAATCGCCCATTCAGGAAGGAACCAAAGGTCATCACCGAGATATTCAGCATAAAGAAATAACCGAAATTCTCCGGCTTAATGCCGTAAATGCCGATATaca                                                                           <  1:274667‑M1/209‑1 (MQ=255)                                                                                                                                                   ccctagtttattcctttttctcattttgttcggtgttttacaTGCCCAAAAGCCCAAATTCAGCAACAGCACCAGAACCAACCAAATTGCCGCCAAAAACTGAACCATCAAACCAACGCGCAACATAGTTTCCGCGCCGACCTTATGCACCAATCGCCCATTCAGGAAGGAACCAAAGGTCATCACCGAGATATTCAGCATAAAGAAATAACCGAAATTCTCCGGCTTAATGCCGTAAATGCCGATATac                                                                            <  1:170403‑M1/208‑1 (MQ=255)                                                                                                                                                         tttattcctttttctcattttgttcggtgttttacaTGCCCAAAAGCCCAAATTCAGCAACAGCACCAGAACCAACCAAATTGCCGCCAAAAACTGAACCATCAAACCAACGCGCAACATAGTTTCCGCGCCGACCTTATGCACCAATCGCCCATTCAGGAAGGAACCAAAGGTCATCACCGAGATATTCAGCATAAAGAAATAACCGAAATTCTCCGGCTTAATGCCGTAAATGCCGATATACACAAt                                                                       >  1:634167‑M1/37‑249 (MQ=255)                                                                                                                                                            attcctttttctcattttgttcggtgttttacaTGCCCAAAAGCCCAAATTCAGCAACAGCACCAGAACCAACCAAATTGCCGCCAAAAACTGAACCATCAAACCAACGCGCAACATAGTTTCCGCGCCGACCTTATGCACCAATCGCCCATTCAGGAAGGAACCAAAGGTCATCACCGAGATATTCAGCATAAAGAAATAACCGAAATTCTCCGGCTTAATGCCGTAAATGCCGATATACACAATAGAcc                                                                  >  1:561024‑M1/34‑251 (MQ=255)                                                                                                                                                            attcctttttctcattttgttcggtgttttacaTGCCCAAAAGCCCAAATTCAGCAACAGCACCAGAACCAACCAAATTGCCGCCAAAAACTGAACCATCAAACCAACGCGCAACATAGTTTCCGCGCCGACCTTATGCACCAATCGCCCATTCAGGAAGGAACCAAAGGTCATCACCGAGATATTCAGCATAAAGAAATAACCGAAATTCTCCGGCTTAATGCCGTAAATGCCGATATACACAATAGAcc                                                                  >  1:697935‑M1/34‑251 (MQ=255)                                                                                                                                                             ttcctttttctcattttgttcggtgttttacaTGCCCAAAAGCCCAAATTCAGCAACAGCACCAGAACCAACCAAATTGCCGCCAAAAACTGAACCATCAAACCAACGCGCAACATAGTTTCCGCGCCGACCTTATGCACCAATCGCCCATTCAGGAAGGAACCAAAGGTCATCACCGAGATATTCAGCATAAAGAAATAACCGAAATTCTCCGGCTTAATGCCGTAAATGCCGATATacac                                                                          <  1:79665‑M1/210‑1 (MQ=255)                                                                                                                                                                ctttttctcattttgttcggtgttttacaTGCCCAAAAGCCCAAATTCAGCAACAGCACCAGAACCAACCAAATTGCCGCCAAAAACTGAACCATCAAACCAACGCGCAACATAGTTTCCGCGCCGACCTTATGCACCAATCGCCCATTCAGGAAGGAACCAAAGGTCATCACCGAGATATTCAGCATAAAGAAATAACCGAAATTCTCCGGCTTAATGCCGTAAATGCCGATATACACAATAGAcccc                                                                <  1:856183‑M1/220‑1 (MQ=255)                                                                                                                                                                  ttttctcattttgttcggtgttttacaTGCCCAAAAGCCCAAATTCAGCAACAGCACCAGAACCAACCAAATTGCCGCCAAAAACTGAACCATCAAACCAACGCGCAACATAGTTTCCGCGCCGACCTTATGCACCAATCGCCCATTCAGGAAGGAACCAAAGGTCATCACCGAGat                                                                                                                                      >  1:20038‑M1/28‑177 (MQ=255)                                                                                                                                                                                   gtgttttacaTGCCCAAAAGCCCAAATTCAGCAACAGCACCAGAACCAACCAAATTGCCGCCAAAAACTGAACCATCAGACCAACGCGCAACATAGTTTCCGCGCCGACCTTATGCACCAATCGCCTATTCAGGAAGGAACCAAAGGTCATCACCGAGATATTCAGCATAAAGAAATAACCGAAATTCTCCGGCTTAATGCCGTAAATGCCGATATACACAAt                                                                       >  1:282712‑M1/11‑223 (MQ=255)                                                                                                                                                                                   gtgttttacaTGCCCAAAAGCCCAAATTCAGCAACAGCACCAGAACCAACCAAATTGCCGCCAAAAACTGAACCATCAAACCAACGCGCAACATAGTTTCCGCGCCGACCTTATGCACCAATCGCCCATTCAGGAAGGAACCAAAGGTCATCACCGAGATATTCAGCATAAAGAAATAACCGAAATTCTCCGGCTTAATGCCGTAAATGCCGATATACACAAt                                                                       >  1:469450‑M1/11‑223 (MQ=255)                                                                                                                                                                                   gtgttttacaTGCCCAAAAGCCCAAATTCAGCAACAGCACCAGAACCAACCAAATTGCCGCCAAAAACTGAACCATCAAACCAACGCGCAACATAGTTTCCGCGCCGACCTTATGCACCAATCGCCCATTCAGGAAGGAACCAAAGGTCATCACCGAGATATTCAGCATAAAGAAATAACCGAAATTCTCCGGCTTAATGCCGTAAATGCCGATATACACAATAGACCCCGCCGTGATGAAAGAAAATAAg                                           <  1:535167‑M1/241‑1 (MQ=255)                                                                                                                                                                                        ttacaTGCCCAAAAGCCCAAATTCAGCAACAGCACCAGAACCAACCAAATTGCCGCCAAAAACTGAACCATCAAACCAACGCGCAACATAGTTTCCGCGCCGACCTTATGCACCAATCGCCCATTCAGGAAGGAACCAAAGGTCATCACCGAGATATTCAGCATAAAGAAATAACCGAAATTCTCCGGCTTAATGCCGTAAATGCCGatat                                                                              >  1:305983‑M1/6‑211 (MQ=255)                                                                                                                                                                                               cccAAAAGCCCAAATTCAGCAACAGCACCAGAACCAACCAAATTGCCGCCAAAAACTGAACCATCAAACCAACGCGCAACATAGTTTCCGCGCCGACCTTATGCACCAATCGCCCATTCAGGAAGGAACCAAAGGTCATCAc                                                                                                                                            <  1:368420/142‑1 (MQ=255)                                                                                                                                                                                                   aaaGCCAAAATTCAGCAACAGCACCAGAACCAACCAAATTGCCGCCAAAAACTGAACCATCAAACCAACGCGCAACATAGTTTCCGCGCCGACCTTATGCACCAATCGCCCATTCAGGAAGGAACCAAAGGTCATCACCGAGATATTCAGCATAAAGAAATAACCGAAATTCTCCGGCTTAATGCCGTAAATGCCGATATACACAATAGACCCCGCCGTGATGAAAGAAAATAAGCCCCCGAAAGCAAaag                           <  1:399684/251‑1 (MQ=255)                                                                                                                                                                                                      gCCCAAATTCAGCAACAGCACCAGAACCAACCAAATTGCCGCCAAAAACTGAACCATCAAACCAACGCGCAACATAGTTTCCGCGCCGACCTTATGCACCAATCGCCCATTCAGGAAGGAACCAAAGGTCATCACCGAGATATTCAGCATAAAGAAATAACCGAAATTCTCCGGCTTAATGCCGTAAATGCCGATATACACAATAGAcc                                                                  >  1:218690/1‑209 (MQ=255)                                                                                                                                                                                                             ttCAGCAACAGCACCAGAACCAACCAAATTGCCGCCAAAAACTGAACCATCAAACCAACGCGCAACATAGTTTCCGCGCCGACCTTATGCACCAATCGCCCATTCAGGAAGGAACCAAAGGTCATCACCGAGATATTCAGCATAAAGAAATAACCGAAATTCTCCGGCTTAATGCCGTAAATGCCGATATacac                                                                          >  1:889312/1‑194 (MQ=255)                                                                                                                                                                                                             ttCAGCAACAGCACCAGAACCAACCAAATTGCCGCCAAAAACTGAACCATCAAACCAACGCGCAACATAGTTTCCGCGCCGACCTTATGCACCAATCGCCCATTCAGGAAGGAACCAAAGGTCATCACCGAGATATTCAGCATAAAGAAATAACCGAAATTCTCCGGCTTAATGCCGTAAATGCCGATATACACAAt                                                                       >  1:187513/1‑197 (MQ=255)                                                                                                                                                                                                                        cACCAGAACCAACCAAATTGCCGCCAAAAACTGAACCATCAAACCAACGCGCAACATAGTTTCCGCGCCGACCTTATGCACCAATCGCCCATTCAGGAAGGAACCAAAGGTCATCACCGAGATATTCAGCATAAAGAAATAACCGAAATTCTCCGGCTTAATGCCGTAAATGCCGATATACACAAt                                                                       >  1:166105/1‑186 (MQ=255)                                                                                                                                                                                                                        cACCAGAACCAACCAAATTGCCGCCAAAAACTGAACCATCAAACCAACGCGCAACATAGTTTCCGCGCCGACCTTATGCACCAATCGCCCATTCAGGAAGGAACCAAAGGTCATCACCGAGATATTCAGCATAAAGAAATAACCGAAATTCTCCGGCTTAATGCCGTAAATGCCGATATACACAATAg                                                                     >  1:718642/1‑188 (MQ=255)                                                                                                                                                                                                                        cACCAGAACCAACCAAATTGCCGCCAAAAACTGAACCATCAAACCAACGCGCAACATAGTTTCCGCGCCGACCTTATGCACCAATCGCCCATTCAGGAAGGAACCAAAGGTCATCACCGAGATATTCAGCATAAAGAAATAACCGAAATTCTCCGGCTTAATGCCGTAAATGCCGATATACACAATAGACCCCGCCGTGATGAAAGAAAATAAGCCCCCGAAAGCAAAAGAAGCGGCGAACATATAcccc       <  1:467261/250‑1 (MQ=255)                                                                                                                                                                                                                        cACCAGAACCAACCAAATTGCCGCCAAAAACTGAACCATCAAACCAACGCGCAACATAGTTTCCGCGCCGACCTTATGCACCAATCGCCCATTCAGGAAGGAACCAAAGGTCATCACCGAGATATTCAGCATAAAGAAATAACCGAAATTCTCCGGCTTAATGCCGTAAATGCCGATATACACAATAGACCCCGCCGTGATGAAAGAAAATAAGCCCCCGAAAGCAAAAGAAGCGGCGAACATATAccc        <  1:572976/249‑1 (MQ=255)                                                                                                                                                                                                                        cACCAGAACCAACCAAATTGCCGCCAAAAACTGAACCATCAAACCAACGCGCAACATAGTTTCCGCGCCGACCTTATGCACCAATCGCCCATTCAGGAAGGAACCAAAGGTCATCACCGAGATATTCAGCATAAAGAAATAACCGAAATTCTCCGGCTTAATGCCGTAAATGCCGATATACACAATAGACCCCGCCGTGATGAAAGAAAATAAGCCCCCGAAAGCAAAAGAAGCGGCGAACATATAccc        <  1:519211/249‑1 (MQ=255)                                                                                                                                                                                                                        cACCAGAACCAACCAAATTGCCGCCAAAAACTGAACCATCAAACCAACGCGCAACATAGTTTCCGCGCCGACCTTATGCACCAATCGCCCATTCAGGAAGGAACCAAAGGTCATCACCGAGATATTCAGCATAAAGAAATAACCGAAATTCTCCGGCTTAATGCCGTAAATGCCGATATACACAATAGACCCCGCCGTGATGAAAGAAAATAAGCCCCCGAAAGCAAAAGAAGCGGCGAACATATAcc         <  1:112726/248‑1 (MQ=255)                                                                                                                                                                                                                        cACCAGAACCAACCAAATTGCCGCCAAAAACTGAACCATCAAACCAACGCGCAACATAGTTTCCGCGCCGACCTTATGCACCAATCGCCAATTCAGGAAGGAACCAAAGGTCATCACCGAGATATTCAGCATAAAGAAATAACCGAAATTCTCCGGCTTAATGCCGTAAATGCCGATATACACAATAGACCCCGCCGTGATGAAAGAAAATAAGCCCCCGAAAGCAAAAGAAGCGGCGAACATATAc          <  1:594132/247‑1 (MQ=255)                                                                                                                                                                                                                          ccAGAACCAACCAAATTGCCGCCAAAAACTGAACCATCAACCCAACGCGCAACATAGTTTCCGCGCCGACCTTATGCACCAATCGCCCATTCAGGAAGGAACCAAAGGTCATCACCGAGATATTCAGCATAAAGAAATAACCGAAATTCTCCGGCTTAATGCCGTAAATGCCGATATACACAATAGACCCCGCCGTGATGAAAGAAAATAAGCCCCCGAAAGCAAAAGAAGCGGCGAACATATACCCCAGc    <  1:265878/251‑1 (MQ=255)                                                                                                                                                                                                                          ccAGAACCAACCAAATTGCCGCCAAAAACTGAACCATCAAACCAACGCGCAACATAGTTTCCGCGCCGACCTTATGCACCAATCGCCCATTCAGGAAGGAACCAAAGGTCATCACCGAGATATTCAGCATAAAGAAATAACCGAAATTCTCCGGCTTAATGCCGTAAATGCCGATATACACAATAGACCCCGCCGTGATGAAAGAAAATAAGCCCCCGAAAGCAAAAGAAGCGGCGAACATATACCCCAGc    <  1:344846/251‑1 (MQ=255)                                                                                                                                                                                                                             gAACCAACCAAATTGCCGCCAAAAACTGAACCATCAAACCAACGCGCAACATAGTTTCCGCGCCGACCTTATGCACCAATCGCCCATTCAGGAAGGAACCAAAGGTCATCACCGAGATATTCAGCATAAAGAAATAACCGAAATTCTCCGGCTTAATGCCGTAAATGCCGATATACACAATAGACCCCGCCGTGATGAAAGAAAATAAGCCCCCGAAAGCAAAAGAAGCGGCGAACATATACCCCAGCAc  >  1:479680/1‑250 (MQ=255)                                                                                                                                                                                                                                  aaccaaATTGCCGCCAAAAACTGAACCATCAAACCAACGCGCAACATAGTTTCCGCGCCGACCTTATGCACCAATCGCCCATTCAGGAAGGAACCAAAGGTCATCACCGAGATATTCAGCATAAAGAAATAACCGAAATTCTCCGGCTTAATGCCGTAAATGCCGATATACACAAt                                                                       >  1:631549/1‑176 (MQ=255)                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                            TGGCGTTGCGCTGCTCATATTCATAAGGGCAACCAGTGTCCCACGCAGGCGCCGGTGCCGAAACGTACACTACCGACCATAGAATTTGCCGAGCAGGCGGCATTCGGGAATTTTTCTAAAATGGACGCCATGGCGTTGGAAGAAATCAGCGAGTTTTGTCCCACAAAAATCGCAATGCCAATTGCAATTGCCCAAAAGCCCAAATTCAGCAACAGCACCAGAACCAACCAAATTTCCGCCAAAAACTGAACCATCAAACCAACGCGCAACATAGTTTCCGCGCCGACCTTATGCACCAATCGCCCATTCAGGAAGGAACCAAAGGTCATCACCGAGATATTCAGCATAAAGAAATAACCGAAATTCTCCGGCTTAATGCCGTAAATGCCGATATACACAATAGACCCCGCCGTGATGAAAGAAAATAAGCCCCCGAAAGCAAAAGAAGCGGCGAACATATACCCCAGCAC  >  1/26680‑27149

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑