Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,670,064	A→T	intergenic (‑116/‑49819)	unknown ← / → unknown	tRNA‑Arg‑CCG/tRNA‑Asn‑GTT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,670,064	0	A	T	100.0%	30.5 / NA	13	intergenic (‑116/‑49819)	unknown/unknown	tRNA‑Arg‑CCG/tRNA‑Asn‑GTT
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (9/4);  total (9/4)

CAACTGAGCTATAGCCACCATATTGAATTAGCTGAATTTGCATGACCTTTGGCGCGCTCGACAGGATTTGAACCTGCGACCTTTGGCTCCGGAGGCCAACGCTCTATCCAACTGAGCTACGAGCGCTGGAAGCGTCGTTGCGGGGCGTATATTAAAAGTTTCAAAAGTGCTTGTCTAGTCTTTTTTTATAAATTTTTATTAGCAGCTA‑TTTTTTATTCAAAGTGAATATAATTTATAAATTATCTGATTATTTTTTATTTAATTTTAACTTTTTAATGAAAATATTAATTTCTATTTAATTTTAGAACGTCTTGCAGCACGGCGCTTCATGCGACGTATCCAGCGGGTAATTTTCCACGCCCGTGTAATAGAGTAAGAATATAAGAAGAACAGAATAATGAAAGCAATAAACATCACCCATTCATTAATAT  >  1/1669823‑1670253                                                                                                                                                                                                                                                   |                                                                                                                                                                                              cAACTGAGCTATAGCCACCATATTGAATTAGCTGAATTTGCATGACCTTTGGCGCGCTCGACAGGATTTGAACCTGCGACCTTTGGCTCCGGAGGCCAACGCTCTATCCAACTGAGCTACGAGCGCTGGAAGCGTCGTTGCGGGGCGTATATTAAAAGTTTCAAAAGTGCTTGTCTAGTCTTTTTTTATAAATTTTTATTAGCAGCTATTTTTTTATTCAAAGTGAATATAATTTATAAATTTTCTGatta                                                                                                                                                                                       >  1:542419/1‑251 (MQ=255)                    atatTGAATTAGCTGAATTTGCATGACCTTTGGCGCGCTCGACAGGATTTGAACCTGCGACCTTTGGCTCCGGAGGCCAACGCTCTATCCAACTGAGCTACGAGCGCTGGAAGCGTCGTTGCGGGGCGTATATTAAAAGTTTCAAAAGTGCTTGTCTAGTCTTTTTTTATAAATTTTTATTAGCAGCTATTTTTTTATTCAAAGTGAATATAATTTATAAATTTTCTGATTATTTTTTATTTAATTTTaa                                                                                                                                                                     >  1:757450/1‑250 (MQ=255)                                                                 gATTTGAACCTGCGACCTTTGGCTCCGGAGGCCAACGCTCTATCCAACTGAGCTACGAGCGCTGGAAGCGTCGTTGCGGGGCGTATATTAAAAGTTTCAAAAGTGCTTGTCTAGTCTTTTTTTATAAATTTTTATTAGCAGCTATTTTTTTATTCAAAGTGAATATAATTTATAAATTTTCTGATTATTTTTTATTTAATTTTAACTTTTTAATGAAAATATTAATTTCTATTTAATTTTAGAACGTCTTg                                                                                                                       >  1:390917/1‑251 (MQ=255)                                                                    ttGAACCTGCGACCTTTGGCTCCGGAGGCCAACGCTCTATCCAACTGAGCTACGAGCGCTGGAAGCGTCGTTGCGGGGCGTATATTAAAAGTTTCAAAAGTGCTTGTCTAGTCTTTTTTTATAAATTTTTATTAGCAGCTATTTTTTTATTCAAAGTGAATATAATTTATAAATTTTCTGATTATTTTTTATTTAATTTTAACTTTTTAATGAAAATATTAATTTCTATTTAATTTTAGAACGTCTTgcag                                                                                                                    <  1:417979/251‑1 (MQ=255)                                                                                                             cAACTGAGCTACGAGCGCTGGAAGCGTCGTTGCGGGGCGTATATTAAAAGTTTCAAAAGTGCTTGTCTAGTCTTTTTTTATAAATTTTTATTAGCAGCTATTTTTTTATTCAAAGTGAATATAATTTATAAATTTTCTGATTATTTTTTATTTAATTTTAACTTTTTAATGAAAATATTAATTTCTATTTAATTTTAGAACGTCTTGCAGCACGGCGCTTCATGCGACGTATCCAGCGGGTAATTTTCCa                                                                            >  1:520762/1‑250 (MQ=255)                                                                                                                    gCTACGAGCGCTGGAAGCGTCGTTGCGGGGCGTATATTAAAAGTTTCAAAAGTGCTTGTCTAGTCTTTTTTTATAAATTTTTATTAGCAGCTATTTTTTTATTCAAAGTGAATATAATTTATAAATTTTCTGATTATTTTTTATTTAATTTTAACTTTTTAATGAAAATATTAATTTCTATTTAATTTTAGAACGTCTTGCAGCACGGCGCTTCATGCGACGTATCCAGCGGGTAATTTTCCACGCCCgtg                                                                    >  1:818525/1‑251 (MQ=255)                                                                                                                            cgcTGGAAGCGTCGTTGCGGGGCGTATATTAAAAGTTTCAAAAGTGCTTGTCTAGTCTTTTTTTATAAATTTTTATTAGCAGCTATTTTTTTATTCAAAGTGAATATAATTTATAAATTTTCTGATTATTTTTTATTTAATTTTAACTTTTTAATGAAAATATTAATTTCTATTTAATTTTAGAACGTCTTGCAGCACGGCGCTTCATGCGACGTATCCAGCGGGTAATTTTCCACGCCCGTGTAATag                                                              <  1:483895/249‑1 (MQ=255)                                                                                                                            cgcTGGAAGCGTCGTTGCGGGGCGTATATTAAAAGTTTCAAAAGTGCTTGTCTAGTCTTTTTTTATAAATTTTTATTAGCAGCTATTTTTTTATTCAAAGTGAATATAATTTATAAATTTTCTGATTATTTTTTATTTAATTTTAACTTTTTAATGAAAATATTAATTTCTATTTAATTTTAGAACGTCTTGCAGCACGGCGCTTCATGCGACGTATCCAGCGGGTAATTTTCCACGCCCGTGTAATag                                                              <  1:700944/249‑1 (MQ=255)                                                                                                                                      gtcgtTGCGGGGCGTATATTAAAAGTTTCAAAAGTGCTTGTCTAGTCTTTTTTTATAAATTTTTATTAGCAGCTATTTTTTTATTCAAAGTGAATATAATTTATAAATTTTCTGATTATTTTTTATTTAATTTTAACTTTTTAATGAAAATATTAATTTCTATTTAATTTTAGAACGTCTTGCAGCACGGCGCTTCATGCGACGTATCCAGCGGGTAATTTTCCACGCCCGTGTAATag                                                              >  1:463586/1‑239 (MQ=255)                                                                                                                                                                                                                   tttttATTCAAAGTGAATATAATTTATAAATTTTCTGATTATTTTTTATTTAATTTTAACTTTTTAATGAAAATATTAATTTCTATTTAATTTTAGAACGTCTTGCAGCACGGCGCTTCATGCGACGTATCCAGCGGGTAATTTTCCACGCCCGTGTAATAGAGTAAGAATATAAGAAGAACAGAATAATGAAAGCAATAAACATCACCCATTCATTAatat  >  1:14629/1‑222 (MQ=255)                                                                                                                                                                                                                               gTGAATATAATTTATAAATTTTCTGATTATTTTTTATTTAATTTTAACTTTTTAATGAAAATATTAATTTCTATTTAATTTTAGAACGTCTTGCAGCACGGCGCTTCATGCGACGTATCCAGCGGGTAATTTTCCACGCCCGTGTAATAGAGTAAGAATATAAGAAGAACAGAATAATGAAAGCAATAAACATCGCCCATTCATTAatat  <  1:625478/210‑1 (MQ=255)                                                                                                                                                                                                                                 gAATATAATTTATAAATTTTCTGATTATTTTTTATTTAATTTTAACTTTTTAATGAAAATATTAATTTCTATTTAATTTTAGAACGTCTTGCAGCACGGCGCTTCATGCGACGTATCCAGCGGGTAATTTTCCACGCCCGTGTAATagag                                                            >  1:364153/1‑150 (MQ=255)                                                                                                                                                                                                                                 gAATATAATTTATAAATTTTCTGATTATTTTTTATTTAATTTTAACTTTTTAATGAAAATATTAATTTCTATTTAATTTTAGAACGTCTTGCAGCACGGCGCTTCATGCGACGTATCCAGCGGGTAATTTTCCACGCCCGTGTAATAGAGTAAGAATATAAGAAGAACAGAATAATGAAAGCAATAAACATCACCCATTCATTAatat  >  1:417170/1‑208 (MQ=255)                                                                                                                                                                                                                                                   |                                                                                                                                                                                              CAACTGAGCTATAGCCACCATATTGAATTAGCTGAATTTGCATGACCTTTGGCGCGCTCGACAGGATTTGAACCTGCGACCTTTGGCTCCGGAGGCCAACGCTCTATCCAACTGAGCTACGAGCGCTGGAAGCGTCGTTGCGGGGCGTATATTAAAAGTTTCAAAAGTGCTTGTCTAGTCTTTTTTTATAAATTTTTATTAGCAGCTA‑TTTTTTATTCAAAGTGAATATAATTTATAAATTATCTGATTATTTTTTATTTAATTTTAACTTTTTAATGAAAATATTAATTTCTATTTAATTTTAGAACGTCTTGCAGCACGGCGCTTCATGCGACGTATCCAGCGGGTAATTTTCCACGCCCGTGTAATAGAGTAAGAATATAAGAAGAACAGAATAATGAAAGCAATAAACATCACCCATTCATTAATAT  >  1/1669823‑1670253

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑