Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,660,130	A→G	intergenic (+18291/+4245)	unknown → / ← unknown	tRNA‑Gly‑GCC/LSU rRNA ## 23S rRNA, large subunit ribosomal RNA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,660,130	0	A	G	100.0%	227.4 / NA	81	intergenic (+18291/+4245)	unknown/unknown	tRNA‑Gly‑GCC/LSU rRNA ## 23S rRNA, large subunit ribosomal RNA
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (35/46);  total (35/46)

TTACCAACTTATCCAACTGGTGCAGCGAAAAATTTGATCAAATTATGGATGAGGCATTGGATACTTCCAATCAGCGCGTACGCTCCGCTGCTTATAATCAGGCACAGACGTTAATTTTATCGGAGTTACCAATCATTCCGATCGCGAACGTTAAGCGGGTTCTCGTCGCCAGTTCGCGTGTTCAAAATATCGACATGAGTCCGTTCGGTAGCCTGAACTTTTCAACCCTCTCCCTGCGAAAAGGACACAAATAATGTTTAATTCACTGATTCGGCAATGTGTTTTTCTCGTTTTAACGTCAATTATCCTCACATTGATAAGTTACGTTATTTTAATGCAGGATCCATTAAATGCCGAACTGGCGACACCGTATTTTTACAATGGCTACCTTAATTATTTAGGCAACCTTACCCGTGGTGATTTAGGTATCAGTTACAATGGCGGGCAGCTGTTAAAATCCACGATTTTTACTGTTTTACCCCCCA  >  1/1659886‑1660370                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                 ttACCAACTTATCCAACTGGTGCAGCGAAAAATTTGATCAAATTATGGATGAGGCATTGGATACTTCCAATCAGCGCGTACGCTCCGCTGCTTATAATCAGGCACAGACGTTAATTTTATCGGAGTTACCAATCATTCCGATCGCGAACGTTAAGCGGGTTCTCGTCGCCAGTTCGCGTGTTCAAAATATCGACATGAGTCCGTTCGGTAGCCTGAACTTTTCAACCCTCTCCCTGCGAAAAGGGCACaaa                                                                                                                                                                                                                                            <  1:207960/251‑1 (MQ=255)      aaCTTATCCAACTGGTGCAGCGAAAAATTTGATCAAATTATGGATGAGGCATTGGATACTTCCAATCAGCGCGTACGCTCCGCTGCTTATAATCAGGCACAGACGTTAATTTTATCGGAGTTACCAATCATTCCGATCGCGAACGTTAAGCGGGTTCTCGTCGCCAGTTCGCGTGTTCAAAATATCGACATGAGTCCGTTCGGTAGCCTGAACTTTTCAACCCTCTCCCTGCGAAAAGGGCACAAATAATg                                                                                                                                                                                                                                       >  1:661719/1‑251 (MQ=255)        cTTATCCAACTGGTGCAGCGAAAAATTTGATCAAATTATGGATGAGGCATTGGATACTTCCAATCAGCGCGTACGCTCCGCTGCTTATAATCAGGCACAGACGTTAATTTTATCGGAGTTACCAATCATTCCGATCGCGAACGTTAAGCGGGTTCTCGTCGCCAGTTCGCGTGTTCAAAATATCGACATGAGTCCGTTCGGTAGCCTGAACTTTTCAACCCTCTCCCTGCGAAAAGGGCACAAATAATGtt                                                                                                                                                                                                                                     >  1:154720/1‑251 (MQ=255)         ttATCCAACTGGTGCAGCGAAAAATTTGATCAAATTATGGATGAGGCATTGGATACTTCCAATCAGCGCGTACGCTCCGCTGCTTATAATCAGGCACAGACGTTAATTTTATCGGAGTTACCAATCATTCCGATCGCGAACGTTAAGCGGGTTCTCGTCGCCAGTTCGCGTGTTCAAAATATCGACATGAGTCCGTTCGGTAGCCTGAACTTTTCAACCCTCTCCCTGCGAAAAGGGCACAAATAATGttt                                                                                                                                                                                                                                    <  1:813157/251‑1 (MQ=255)         ttATCCAACTGGTGCAGCGAAAAATTTGATCAAATTATGGATGAGGCATTGGATACTTCCAATCAGCGCGTACGCTCCGCTGCTTATAATCAGGCACAGACGTTAATTTTATCGGAGTTACCAATCATTCCGATCGCGAACGTTAAGCGGGTTCTCGTCGCCAGTTCGCGTGTTCAAAATATCGACATGAGTCCGTTCGGTAGCCTGAACTTTTCAACCCTCTCCCTGCGAAAAGGGCACAAATAATGttt                                                                                                                                                                                                                                    <  1:238363/251‑1 (MQ=255)          tATCCAACTGGTGCAGCGAAAAATTTGATCAAATTATGGATGAGGCATTGGATACTTCCAATCAGCGCGTACGCTCCGCTGCTTATAATCAGGCACAGACGTTAATTTTATCGGAGTTACCAATCATTCCGATCGCGAACGTTAAGCGGGTTCTCGTCGCCAGTTCGCGTGTTCAAAATATCGACATGAGTCCGTTCGGTAGCCTGAACTTTTCAACCCTCTCCCTGCGAAAAGGGCACAAATAATg                                                                                                                                                                                                                                       >  1:597416/1‑247 (MQ=255)               aaCTGGTGCAGCGAAAAATTTGATCAAATTATGGATGAGGCATTGGATACTTCCAATCAGCGCGTACGCTCCGCTGCTTATAATCAGGCACAGACGTTAATTTTATCGGAGTTACCAATCATTCCGATCGCGAACGTTAAGCGGGTTCTCGTCGCCAGTTCGCGTGTTCAAAATATCGACATGAGTCCGTTCGGTAGCCTGAACTTTTCAACCCTCTCCCTGCGAAAAGGGCACAAATAATg                                                                                                                                                                                                                                       >  1:129836/1‑242 (MQ=255)                aCTGGTGCAGCGAAAAATTTGATCAAATTATGGATGAGGCATTGGATACTTCCAATCAGCGCGTACGCTCCGCTGCTTATAATCAGGCACAGACGTTAATTTTATCGGAGTTACCAATCATTCCGATCGCGAACGTTAAGCGGGTTCTCGTCGCCAGTTCGCGTGTTCAAAATATCGACATGAGTCCGTTCGGTAGCCTGAACTTTTCAACCCTCTCCCTGCGAAAAGGGCACAAATAATGttt                                                                                                                                                                                                                                    <  1:149498/244‑1 (MQ=255)                     tGCAGCGAAAAATTTGATCAAATTATGGATGAGGCATTGGATACTTCCAATCAGCGCGTACGCTCCGCTGCTTATAATCAGGCACAGACGTTAATTTTATCGGAGTTACCAATCATTCCGATCGCGAACGTTAAGCGGGTTCTCGTCGCCAGTTCGCGTGTTCAAAATATCGACATGAGTCCGTTCGGTAGCCTGAACTTTTCAACCCTCTCCCTGCGAAAAGGGCACAAATAATGTTTAATTCACTGAtt                                                                                                                                                                                                                        <  1:596716/251‑1 (MQ=255)                         gCGAAAAATTTGATCAAATTATGGATGAGGCATTGGATACTTCCAATCAGCGCGTACGCTCCGCTGCTTATAATCAGGCACAGACGTTAATTTTATCGGAGTTACCAATCATTCCGATCGCGAACGTTAAGCGGGTTCTCGTCGCCAGTTCGCGTGTTCAAAATATCGACATGAGTCCGTTCGGTAGCCTGAACTTTTCAACCCTCTCCCTGCGAAAAGGGCACAAATAATGTTTAATTCACTGATTCgg                                                                                                                                                                                                                     >  1:690162/1‑250 (MQ=255)                                         aaTTATGGATGAGGCATTGGATACTTCCAATCAGCGCGTACGCTCCGCTGCTTATAATCAGGCATAGACGTTAATTTTATCGGAGTTACCAATCATTCCGATCGCGAACGTTAAGCGGGTTCTCGTCGCCAGTTCGCGTGTTCAAAATATCGACATGAGTCCGTTCGGTAGCCTGAACTTTTCAACCCTCTCCCTGCGAAAAGGGCACAAATAATGTTTAATTCACTGATTCgg                                                                                                                                                                                                                     <  1:443038/234‑1 (MQ=255)                                         aaTTATGGATGAGGCATTGGATACTTCCAATCAGCGCGTACGCTCCGCTGCTTATAATCAGGCACAGACGTTAATTTTATCGGAGTTACCAATCATTCCGATCGCGAACGTTAAGCGGGTTCTCGTCGCCAGTTCGCGTGTTCAAAATATCGACATGAGTCCGTTCGGTAGCCTGAACTTTTCAACCCTCTCCCTGCGAAAAGGGCACAAATAATGTTTAATTCACTGATTCGGCaa                                                                                                                                                                                                                  <  1:412363/237‑1 (MQ=255)                                         aaTTATGGATGAGGCATTGGATACTTCCAATCAGCGCGTACGCTCCGCTGCTTATAATCAGGCACAGACGTTAATTTTATCGGAGTTACCAATCATTCCGATCGCGAACGTTAAGCGGGTTCTCGTCGCCAGTTCGCGTGTTCAAAATATCGACATGAGTCCGTTCGGTAGCCTGAACTTTTCAACCCTCTCCCTGCGAAAAGGGCACAAATAATGTTTAATTCACTGATTCGGCAATGTGTTTTTCTCGt                                                                                                                                                                                                    >  1:138344/1‑251 (MQ=255)                                               ggATGAGGCATTGGATACTTCCAATCAGCGCGTACGCTCCGCTGCTCATAATCAGGCACAGACGTTAATTTTATCGGAGTTACCAATCATTCCGATCGCGAACGTTAAGCGGGTTCTCGTCGCCAGTTCGCGTGTTCAAAATATCGACATGAGTCCGTTCGGTAGCCTGAACTTTTCAACCCTCTCCCTGCGAAAAGGGCACAAATAATGTTTAAt                                                                                                                                                                                                                                 <  1:424425/216‑1 (MQ=255)                                                gATGAGGCATTGGATACTTCCAATCAGCGCGTACGCTCCGCTGCTTATAATCAGGCACAGACGTTAATTTTATCGGAGTTACCAATCATTCCGATCGCGAACGTTAAGCGGGTTCTCGTCGCCAGTTCGCGTGTTCAAAATATCGACATGAGTCCGTTCGGTAGCCTGAACTTTTCAACCCTCTCCCTGCGAAAAGGGCACAAATAATGTTTAATTCACTGATTCGGCAATGTGTTTTTCTCGTTTTAACg                                                                                                                                                                                             >  1:372633/1‑251 (MQ=255)                                                      gCATTGGATACTTCCAATCAGCGCGTACGCTCCGCTGCTTATAATCAGGCACAGACGTTAATTTTATCGGAGTTACCAATCATTCCGATCGCGAACGTTAAGCGGGTTCTCGTCGCCAGTTCGCGTGTTCAAAATATCGACATGAGTCCGTTCGGTAGCCTGAACTTTTCAACCCTCTCCCTGCGAAAAGGGCACAAATAATGTTTAATTCACTGATTCGGCAATGTGTTTTTCTCGTTTTAACGTCAAtt                                                                                                                                                                                       >  1:513328/1‑251 (MQ=255)                                                       cATTGGATACTTCCAATCAGCGCGTACGCTCCGCTGCTTATAATCAGGCACAGACGTTAATTTTATCGGAGTTACCAATCATTCCGATCGCGAACGTTAAGCGGGTTCTCGTCGCCAGTTCGCGTGTTCAAAATATCGACATGAGTCCGTTCGGTAGCCTGAACTTTTCAACCCTCTCCCTGCGAAAAGGGCACAAATAATGTTTAATTCACTGATTCGGCAATGTGTTTTTCTCGTTTTAACGTCAATTa                                                                                                                                                                                      <  1:418455/251‑1 (MQ=255)                                                         ttGGATACTTCCAATCAGCGCGTACGCTCCGCTGCTTATAATCAGGCACAGACGTTAATTTTATCGGAGTTACCAATCATTCCGATCGCGAACGTTAAGCGGGTTCTCGTCGCCAGTTCGCGTGTTCAAAATATCGACATGAGTCCGTTCGGTAGCCTGAACTTTTCAACCCTCTCCCTGCGAAAAGGGCACAAATAATGTTTAATTCACTGATTCGGCAATGTGTTTTTCTCGTTTTAACGTCAATTATc                                                                                                                                                                                    <  1:227050/251‑1 (MQ=255)                                                          tGGATACTTCCAAGCAGCGCGTGCGCTCCGCTGCTTATAATCAGGCACAGACGTTAATTTTATCGGAGTTACCAATCATTCCGATCGCGAACGTTAAGCGGGTTCTCGTCGCCAGTTCGCGTGTTCAAAATATCGACATGAGTCCGTTCGGTAGCCTGAACTTTTCAACCCTCTCCCTGCGAAAAGGGCACAAATAATGTTTAATTCACTGATTCGGCAATGTGTTTTTCTCGTTTTAACGTCAATTATcc                                                                                                                                                                                   <  1:463987/251‑1 (MQ=255)                                                           ggATACTTCCAATCAGCGCGTACGCTCCGCTGCTTATAATCAGGCACAGACGTTAATTTTATCGGAGTTACCAATCATTCCGATCGCGAACGTTAAGCGGGTTCTCGTCGCCAGTTCGCGTGTTCAAAATATCGACATGAGTCCGTTCGGTAGCCTGAACTTTTCAACCCTCTCCCTGCGAAAAGGGCACAAATAATGTTTAATTCACTGATTCGGCAATGTGTTTTTCTCGTTTTAACGTCAATTATc                                                                                                                                                                                    >  1:696260/1‑249 (MQ=255)                                                           ggATACTTCCAATCAGCGCGTACGCTCCGCTGCTTATAATCAGGCACAGACGTTAATTTTATCGGAGTTACCAATCATTCCGATCGCGAACGTTAAGCGGGTTCTCGTCGCCAGTTCGCGTGTTCAAAATATCGACATGAGTCCGTTCGGTAGCCTGAACTTTTCAACCCTCTCCCTGCGAAAAGGGCACAAATAATGTTTAATTCACTGATTCGGCAATGTGTTTTTCTCGTTTTAACGTCAATTATc                                                                                                                                                                                    >  1:285894/1‑249 (MQ=255)                                                                cTTCCAATCAGCGCGTACGCTCCGCTGCTTATAATCAGGCACAGACGTTAATTTTATCGGAGTTACCAATCATTCCGATCGCGAACGTTAAGCGGGTTCTCGTCGCCAGTTCGCGTGTTCAAAATATCGACATGAGTCCGTTCGGTAGCCTGAACTTTTCAACCCTCTCCCTGCGAAAAGGGCACAAATAATGTTTAATTCACTGAtt                                                                                                                                                                                                                        <  1:845223/208‑1 (MQ=255)                                                                    cAATCAGCGCGTACGCTCCGCTGCTTATAATCAGGCACAGACGTTAATTTTATCGGAGTTACCAATCATTCCGATCGCGAACGTTAAGCGGGTTCTCGTCGCCAGTTCGCGTGTTCAAAATATCGACATGAGTCCGTTCGGTAGCCTGAACTTTTCAACCCTCTCCCTGCGAAAAGGGCACAAATAATGTTTAATTCACTGATTCGGCAATGTGTTTTTCTCGTTTTAACGTCAATTATCCTCACATTGAt                                                                                                                                                                         >  1:370959/1‑251 (MQ=255)                                                                    cAATCAGCGCGTACGCTCCGCTGCTTATAATCAGGCACAGACGTTAATTTTATCGGAGTTACCAATCATTCCGATCGCGAACGTTAAGCGGGTTCTCGTCGCCAGTTCGCGTGTTCAAAATATCGACATGAGTCCGTTCGGTAGCCTGAACTTTTCAACCCTCTCCCTGCGAAAAGGGCACAAATAATGTTTAATTCACTGATTCGGCAATGTGTTTTTCTCGTTTTAACGTCAATTATCCTCACATTGAt                                                                                                                                                                         <  1:808246/251‑1 (MQ=255)                                                                      aTCAGCGCGTACGCTCCGCTGCTTATAATCAGGCACAGACGTTAATTTTATCGGAGTTACCAATCATTCCGATCGCGAACGGTAAGCGGGTTCTCGTCGCCAGTTCGCGTGTTCAAAATATCGACATGAGTCCGTTCGGTAGCCTGAACTTTTCAACCCTCTCCCTGCGAAAAGGGCACAAATAATGTTTAATTCACTGATTCGGCAATGTGTTTTTCTCGTTTTAACGTCAATTATCCTCACATTGATaa                                                                                                                                                                       <  1:434476/251‑1 (MQ=255)                                                                           cgcgTACGCTCCGCTGCTTATAATCAGGCACAGACGTTAATTTTATCGGAGTTACCAATCATTCCGATCGCGAACGTTAAGCGGGTTCTCGTCGCCAGTTCGCGTGTTCAAAATATCGACATGAGTCCGTTCGGTAGCCTGAACTTTTCAACCCTCTCCCTGCGAAAAGGGCACAAATAATGTTTAATTCACTGATTCGGCAATGTGTTTTTCTCGTTTTAACGTCAATTATCCTCACATTGATAAGTTa                                                                                                                                                                   <  1:862204/250‑1 (MQ=255)                                                                           cgcgTACGCTCCGCTGCTTATAATCAGGCACAGACGTTAATTTTATCGGAGTTACCAATCATTCCGATCGCGAACGTTAAGCGGGTTCTCGTCGCCAGTTCGCGTGTTCAAAATATCGACATGAGTCCGTTCGGTAGCCTGAACTTTTCAACCCTCTCCCTGCGAAAAGGGCACAAATAATGTTTAATTCACTGATTCGGCAATGTGTTTTTCTCGTTTTAACGTCAATTATCCTCACATTGATAAGTTa                                                                                                                                                                   <  1:692080/250‑1 (MQ=255)                                                                                        ctgctTATAATCAGGCACAGACGTTAATTTTATCGGAGTTACCAATCATTCCGATCGCGAACGTTAAGCGGGTTCTCGTCGCCAGTTCGCGTGTTCAAAATATCGACATGAGTCCGTTCGGTAGCCTGAACTTTTCAACCCTCTCCCTGCGAAAAGGGCACAAATAATGTTTAATTCACTGATTCGGCAATGTGTTTTTCTCGTTTTAACGTCAATTATCCTCACATTGATAAGTTACGTTATTTTAATg                                                                                                                                                      <  1:549905/250‑1 (MQ=255)                                                                                        ctgctTATAATCAGGCACAGACGTTAATTTTATCGGAGTTACCAATCATTCCGATCGCGAACGTTAAGCGGGTTCTCGTCGCCAGTTCGCGTGTTCAAAATATCGACATGAGTCCGTTCGGTAGCCTGAACTTTTCAACCCTCTCCCTGCGAAAAGGGCACAAATAATGTTTAATTCACTGATTCGGCAATGTGTTTTTCTCGTTTTAACGTCAATTATCCTCACATTGATAAGTTACGTTATTTTAATg                                                                                                                                                      <  1:271487/250‑1 (MQ=255)                                                                                          gctTATAATCAGGCACAGACGTTAATTTTATCGGAGTTACCAATCATTCCGATCGCGAACGTTAAGCGGGTTCTCGTCGCCAGTTCGCGTGTTCAAAATATCGACATGAGTCCGTTCGGTAGCCTGAACTTTTCAACCCTCTCCCTGCGAAAAGGGCACAAATAATg                                                                                                                                                                                                                                       >  1:661872/1‑167 (MQ=255)                                                                                          gctTATAATCAGGCACAGACGTTAATTTTATCGGAGTTACCAATCATTCCGATCGCGAACGTTAAGCGGGTTCTCGTCGCCAGTTCGCGTGTTCAAAATATCGACATGAGTCCGTTCGGTAGCCTGAACTTTTCAACCCTCTCCCTGCGAAAAGGGCACAAATAATGt                                                                                                                                                                                                                                      <  1:816357/168‑1 (MQ=255)                                                                                          gctTATAATCAGGCACAGACGTTAATTTTATCGGAGTTACCAATCATTCCGATCGCGAACGTTAAGCGGGTTCTCGTCGCCAGTTCGCGTGTTCAAAATATCGACATGAGTCCGTTCGGTAGCCTGAACTTTTCAACCCTCTCCCTGCGAAAAGGGCACAAATAATGTTTAAt                                                                                                                                                                                                                                 >  1:510367/1‑173 (MQ=255)                                                                                          gctTATAATCAGGCACAGACGTTAATTTTATCGGAGTTACCAATCATTCCGATCGCGAACGTTAAGCGGGTTCTCGTCGCCAGTTCGCGTGTTCAAAATATCGACATGAGTCCGTTCGGTAGCCTGAACTTTTCAACCCTCTCCCTGCGAAAAGGGCACAAATAATGTTTAATTCACTGATTCGGCAATGTGTTTTTCTCGTTTTAACGTCAATTATCCTCACATTGATAAGTTACGtt                                                                                                                                                               <  1:821750/239‑1 (MQ=255)                                                                                          gctTATAATCAGGCACAGACGTTAATTTTATCGGAGTTACCAATCATTCCGATCGCGAACGTTAAGCGGGTTCTCGTCGCCAGTTCGCGTGTTCAAAATATCGACATGAGTCCGTTCGGTAGCCTGAACTTTTCAACCCTCTCCCTGCGAAAAGGGCACAAATAATGTTTAATTCACTGATTCGGCAATGTGTTTTTCTCGTTTTAACGTCAATTATCCTCACATTGATAAGTTACGTTATTTTAATGCAg                                                                                                                                                   >  1:467289/1‑251 (MQ=255)                                                                                          gctTATAATCAGGCACAGACGTTAATTTTATCGGAGTTACCAATCATTCCGATCGCGAACGTTAAGCGGGTTCTCGTCGCCAGTTCGCGTGTTCAAAATATCGACATGAGTCCGTTCGGTAGCCTGAACTTTTCAACCCTCTCCCTGCGAAAAGGGCACAAATAATGTTTAATTCACTGATTCGGCAATGTGTTTTTCTCGTTTTAACGTCAATTATCCTCACATTGATAAGTTACGTTATTTTAATGCAg                                                                                                                                                   >  1:619235/1‑251 (MQ=255)                                                                                          gctTATAATCAGGCACAGACGTTAATTTTATCGGAGTTACCAATCATTCCGATCGCGAACGTTAAGCGGGTTCTCGTCGCCAGTTCGCGTGTTCAAAATATCGACATGAGTCCGTTCGGTAGCCTGAACTTTTCAACCCTCTCCCTGCGAAAAGGGCACAAATAATGTTTAATTCACTGATTCGGCAATGTGTTTTTCTCGTTTTAACGTCAATTATCCTCACATTGATAAGTTACGTTATTTTAATGCAg                                                                                                                                                   >  1:840619/1‑251 (MQ=255)                                                                                          gctTATAATCAGGCACAGACGTTAATTTTATCGGAGTTACCAATCATTCCGATCGCGAACGTTAAGCGGGTTCTCGTCGCCAGTTCGCGTGTTCAAAATATCGACATGAGTCCGTTCGGTAGCCTGAACTTTTCAACCCTCTCCCTGCGAAAAGGGCACAAATAATGTTTAATTCACTGATTCGGCAATGTGTTTTTCTCGTTTTAACGTCAATTATCCTCACATTGATAAGTTACGTTATTTTAATGCAg                                                                                                                                                   >  1:529384/1‑251 (MQ=255)                                                                                          gctTATAATCAGGCACAGACGTTAATTTTATCGGAGTTACCAATCATTCCGATCGCGAACGTTAAGCGGGTTCTCGTCGCCAGTTCGCGTGTTCAAAATATCGACATGAGTCCGTTCGGTAGCCTGAACTTTTCAACCCTCTCCCTGCGAAAAGGGCACAAATAATGTTTAATTCACTGATTCGGCAATGTGTTTTTCTCGTTTTAACGTCAATTATCCTCACATTGATAAGTTACGTTATTTTAATGCAg                                                                                                                                                   >  1:142979/1‑251 (MQ=255)                                                                                          gctTATAATCAGGCACAGACGTTAATTTTATCGGAGTTACCAATCATTCCGATCGCGAACGT‑‑AGCGGGTTCTCGTCGCCAGTTCGCGTGTTCAAAATATCGACATGAGTCCGTTCGGTAGCCTGAACTTTTCAACCCTCTCCCTGCGAAAAGGGCACAAATAATGt                                                                                                                                                                                                                                      >  1:448995/1‑166 (MQ=255)                                                                                             tataATCAGGCACAGACGTTAATTTTATCGGAGTTACCAATCATTCCGATCGCGAACGTTAAGCGGGTTCTCGTCGCCAGTTCGCGTGTTCAAAATATCGACATGAGTCCGTTCGGTAGCCTGAACTTTTCAACCCTCTCCCTGCGAAAAGGGCACAAATAATGTTTAATTCACTGATTCGGCAATGTGTTTTTCTCGTTTTAACGTCAATTATCCTCACATTGATAAGTTACGTTATTTTAATGCAGGAt                                                                                                                                                <  1:537614/251‑1 (MQ=255)                                                                                              ataATCAGGCACAGACGTTAATTTTATCGGAGTTACCAATCATTCCGATCGCGAACGTTAAGCGGGTTCTCGTCGCCAGTTCGCGTGTTCAAAATATCGACATGAGTCCGTTCGGTAGCCTGAACTTTTCAACCCTCTCCCTGCGAAAAGGGCACAAATAATGTTTAATTCACTGATTCGGCAATGTGTTTTTCTCGTTTTAAc                                                                                                                                                                                              <  1:710551/204‑1 (MQ=255)                                                                                                      gCACAGACGTTAATTTTATCGGAGTTACCAATCATTCCGATCGCGAACGTTAAGCGGGTTCTCGTCGCCAGTTCGCGTGTTCAAAATATCGACATGAGTCCGTTCGGTAGCCTGAACTTTTCAACCCTCTCCCTGCGAAAAGGGCACAAATAATGTTTAATTCACTGATTCGGCAATGTGTTTTTCTCGTTTTAACGTCAATTATCCTCACATTGATAAGTTACGTTATTTTAATGCAGGATCCATTAAAt                                                                                                                                       >  1:422999/1‑251 (MQ=255)                                                                                                            aCGTTAATTTTATCGGAGTTACCAATCATTCCGATCGCGAACGTTAAGCGGGTTCTCGTCGCCAGTTCGCGTGTTCAAAATATCGACATGAGTCCGTTCGGTAGCCTGAACTTTTCAACCCTCTCCCTGCGAAAAGGGCACAAATAATGTTTAATTCACTGATTCGGCAATGTGTTTTTCTCGTTTTAACGTCAATTATCCTCACATTGATAAGTTACGTTATTTTAATGCAGGATCCATTAAATGc                                                                                                                                     <  1:500279/247‑1 (MQ=255)                                                                                                             cGTTAATTTTATCGGAGTTACCAATCATTCCGATCGCGAACGTTAAGCGGGTTCTCGTCGCCAGTTCGCGTGTTCAAAATATCGACATGAGTCCGTTCGGTAGCCTGAACTTTTCAACCCTCTCCCTGCGAAAAGGGCACAAATAATGTTTAATTCACTGATTCGGCAATGTGTTTTTCTCGTTTTAACGTCAATTATCCTCACATTGATAAGTTAc                                                                                                                                                                  >  1:39793/1‑217 (MQ=255)                                                                                                                           gAGTTACC‑ATCATTCCGATCGCGAACGTTAAGCGGGTTCTCGTCGCCAGTTCGCGTGTTCAAAATATCGACATGAGTCCGTTCGGTAGCCTGAACTTTTCAACCCTCTCCCTGCGAAAAGGGCACAaat                                                                                                                                                                                                                                           <  1:540270/129‑1 (MQ=255)                                                                                                                             gTTACCAATCATTCCGATCGCGAACGTTAAGCGGGTTCTCGTCGCCAGTTCGCGTGTTCAAAATATCGACATGAGTCCGTTCGGTAGCCTGAACTTTTCAACCCTCTCCCTGCGAAAAGGGCACAAATAATGTTTAATTCACTGATTCGGCAATGTGTTTTTCTCGTTTTAACGTCAATTATCCTCACAt                                                                                                                                                                             >  1:71162/1‑190 (MQ=255)                                                                                                                             gTTACCAATCATTCCGATCGCGAACGTTAAGCGGGTTCTCGTCGCCAGTTCGCGTGTTCAAAATATCGACATGAGTCCGTTCGGTAGCCTGAACTTTTCAACCCTCTCCCTGCGAAAAGGGCACAAATAATGTTTAATTCACTGATTCGGCAATGTGTTTTTCTCGTTTTAACGTCAATTATCCTCACATTGATAAGTTACGTTATTTTAATGCAGGATCCATTAAATGCCGAACTGGCGACACCGTAttt                                                                                                                >  1:34018/1‑251 (MQ=255)                                                                                                                              ttACCAATCATTCCGATCGCGAACGTTAAGCGGGTTCTCGTCGCCAGTTCGCGTGTTCAAAATATCGACATGAGTCCGTTCGGTAGCCTGAACTTTTCAACCCTCTCCCTGCGAAAAGGGCACAAATAATGTTTAATTCACTGATTCGGCAATGTGTTTTTCTCGTTTTAACGTCAATTa                                                                                                                                                                                      <  1:741671/180‑1 (MQ=255)                                                                                                                               tACCAATCATTCCGATCGCGAACGTTAAGCGGGTTCTCGTCGCCAGTTCGCGTGTTCAAAATATCGACATGAGTCCGTTCGGTAGCCTGAACTTTTCAACC‑‑CTCCCTGCGAAAAGGGCACAAATAATGTTTAATTCACTGATTCGGCAATGTGTTTTTCTCGTTTTAACGTCAATTATCCTCACATTGATAAGTTACGTTATTTTAATGCAGGATCCATTAAATGCCGAACTGGCGACACCGTATTTTTAc                                                                                                            <  1:828246/251‑1 (MQ=255)                                                                                                                                 ccAATCATTCCGATCGCGAACGTTAAGCGGGTTCTCGTCGCCAGTTCGCGTGTTCAAAATATCGACATGAGTCCGTTCGGTAGCCTGAACTTTTCAACCCTCTCCCTGCGAAAAGGGCACAAATAATGTTTAATTCACTGATTCGGCAATGTGTTTTTCTCGTTTTAACGTCAATTATCCTCACATTGATAAGTTACGTTATTTTAATGCAGGATCCATTAAATGCCGAACTGGCGACACCGTATTTTTAc                                                                                                            <  1:376333/251‑1 (MQ=255)                                                                                                                                 ccAATCATTCCGATCGCGAACGTTAAGCGGGTTCTCGTCGCCAGTTCGCGTGTTCAAAATATCGACATGAGTCCGTTCGGTAGCCTGAACTTTTCAACCCTCTCCCTGCGAAAAGGGCACAAATAATGTTTAATTCACTGATTCGGCAATGTGTTTTTCTCGTTTTAACGTCAATTATCCTCACATTGATAAGTTACGTTATTTTAATGCAGGATCCATTAAATGCCGAACTGGCGACACCGTATTTTTAc                                                                                                            <  1:176056/251‑1 (MQ=255)                                                                                                                                  cAATCATTCCGATCGCGAACGTTAAGCGGGTTCTCGTCGCCAGTTCGCGTGTTCAAAATATCGACATGAGTCCGTTCGGTAGCCTGAACTTTTCAACCCTCTCCCTGCGAAAAGGGCACAAATAATGTTTAATTCACTGATTCGGCAATGTGTTTTTCTCGTTTTAACGTCAATTATCCTCACATTGATAAGTTACGTTATTTTAATGCAGGATCCATTAAATGCCGAACTGGCGACACCg                                                                                                                     >  1:597104/1‑241 (MQ=255)                                                                                                                                  cAATCATTCCGATCGCGAACGTTAAGCGGGTTCTCGTCGCCAGTTCGCGTGTTCAAAATATCGACATGAGTCCGTTCGGTAGCCTGAACTTTTCAACCCTCTCCCTGCGAAAAGGGCACAAATAATGTTTAATTCACTGATTCGGCAATGTGTTTTTCTCGTTTTAACGTCAATTATCCTCACATTGATAAGTTACGTTATTTTAATGCAGGATCCATTAAATGCCGAACTGGCGACACCGTATTTTTACa                                                                                                           >  1:680299/1‑251 (MQ=255)                                                                                                                                                 cgAACGTTAAGCGGGTTCTCGTCGCCAGTTCGCGTGTTCAAAATATCGACATGAGTCCGTTCGGTAGCCTGAACTTTTCAACCCTCTCCCTGCGAAAAGGGCACAAATAATGTTTAATTCACTGATTCGGCAATGTGTTTTTCTCGTTTTAACGTCAATTATCCTCACATTGATAAGTTACGTTATTTTAATGCAGGATCCATTAAATGCCGAACTGGCGACACCGTATTTTTACAATGGCTACCTTAatt                                                                                            <  1:222816/251‑1 (MQ=255)                                                                                                                                                 cgAACGTTAAGCGGGTTCTCGTCGCCAGTTCGCGTGTTCAAAATATCGACATGAGTCCGTTCGGTAGCCTGAACTTTTCAACCCTCTCCCTGCGAAAAGGGCACAAATAATGTTTAATTCACTGATTCGGCAATGTGTTTTTCTCGTTTTAACGTCAATTATCCTCACATTGATAAGTTACGTTATTTTAATGCAGGATCCATTAAATGCCGAACTGGCGACACCGTATTTTTACAATGGCTACCTTAatt                                                                                            <  1:645259/251‑1 (MQ=255)                                                                                                                                                   aaCGTTAAGCGGGTTCTCGTCGCCAGTTCGCGTGTTCAAAATATCGACATGAGTCCGTTCGGTAGCCTGAACTTTTCAACCCTCTCCCTGCGAAAAGGGCACAAATAATGTTTAATTCACTGATTCGGCAATGTGTTTTTCTCGTTTTAACGTCAATTATCCTCACATTGATAAGTTACGTTATTTTAATGCAGGATCCATTAAATGCCGAACTGGCGACACCGTATTTTTACAATGGCTACCTTAatta                                                                                           <  1:678307/250‑1 (MQ=255)                                                                                                                                                     cGTTAAGCGGGTTCTCGTCGCCAGTTCGCGTGTTCAAAATATCGACATGAGTCCGTTCGGTAGCCTGAACTTTTCAACCCTCTCCCTGCGAAAAGGGCACAAATAATGTTTAATTCACTGATTCGGCAATGTGTTTTTCTCGTTTTAACGTCAATTATCCTcaca                                                                                                                                                                              <  1:804202/165‑1 (MQ=255)                                                                                                                                                     cGTTAAGCGGGTTCTCGTCGCCAGTTCGCGTGTTCAAAATATCGACATGAGTCCGTTCGGTAGCCTGAACTTTTCAACCCTCTCCCTGCGAAAAGGGCACAAATAATGTTTAATTCACTGATTCGGCAATGTGTTTTTCTCGTTTTAACGTCAATTATCCTCACAt                                                                                                                                                                             <  1:87207/166‑1 (MQ=255)                                                                                                                                                      gTTAAGCGGGTTCTCGTCGCCAGTTCGCGTGTTCAAAATATCGACATGAGTCCGTTCGGTAGCCTGAACTTTTCAACCCTCTCCCTGCGAAAAGGGCACAAATAATGTTTAATTCACTGATTCGGCAATGTGTTTTTCTCGTTTTAACGTCAATTATCCTCACATTGATAAGTTACGTTATTTTAATGc                                                                                                                                                     >  1:263947/1‑189 (MQ=255)                                                                                                                                                              ggTTCTCGTCGCCAGTTCGCGTGTTCAAAATATCGACATGAGTCCGTTCGGTAGCCTGAACTTTTCAACCCTCTCCCTGCGAAAAGGGCACAAATAATGTTTAATTCACTGATTCGGCAATGTGTTTTTCTCGTTTTAACGTCAATTATCCTCACATTGATAAGTTACGTTATTTTAATGCAGGAt                                                                                                                                                <  1:36882/186‑1 (MQ=255)                                                                                                                                                                  ctcGTCGCCAGTTCGCGTGTTCAAAATATCGACATGAGTCCGTTCGGTAGCCTGAACTTTTCAACCCTCTCCCTGCGAAAAGGGCACAAATAATGTTTAATTCACTGATTCGGCAATGTGTTTTTCTCGTTTTAACGTCAATTATCCTCACATTGATAAGTTACGTTa                                                                                                                                                              <  1:125858/168‑1 (MQ=255)                                                                                                                                                                   tcgtcgCCAGTTCGCGTGTTCAAAATATCGACATGAGTCCGTTCGGTAGCCTGAACTTTTCAACCCTCTCCCTGCGAAAAGGGCACAAATAATGTTTAATTCACTGATTCGGCAATGTGTTTTTCTCGTTTTAACGTCAATTATCCTCACATTGATAAGTTACGTTATTTTAATGCAGGATCCATTAAATGCCGAACTGGCGACACCGTATTTTTACAATGGCTACCTTAATTATTTAGGCAACCTTAccc                                                                          <  1:659608/251‑1 (MQ=255)                                                                                                                                                                   tcgtcgCCAGTTCGCGTGTTCAAAATATCGACATGAGTCCGTTCGGTAGCCTGAACTTTTCAACCCTCTCCCTGCGAAAAGGGCACAAATAATGTTTAATTCACTGATTCGGCAATGTGTTTTTCTCGTTTTAACGTCAATTATCCTCACATTGATAAGTTACGTTATTTTAATGCAGGATCCATTAAATGCCGAACTGGCGACACCGTATTTTTACAATGGCTACCTTAATTATTTAGGCAACCTTAccc                                                                          <  1:146247/251‑1 (MQ=255)                                                                                                                                                                            gTTCGCGTGTTCAAAATATCGACATGAGTCCGTTCGGTAGCCTGAACTTTTCAACCCTCTCCCTGCGAAAAGGGCACAAATAATGTTTAATTCACTGATTCGGCAATGTGTTTTTCTCGTTTTAACGTCAATTATCCTCACATTGATAAGTTACGTTATTTTAATGCAGAATCCATTAAATGCCGAACTGGCGACACCGTATTTTTACAATGGCTACCTTAATTATTTAGGCAACCTTACCCGTGGTGAtt                                                                 >  1:423286/1‑251 (MQ=255)                                                                                                                                                                               cgcgTGTTCAAAATATCGACATGAGTCCGTTCGGTAGCCTGAACTTTTTAACCCTCTCCCTGCGAAAAGGGCACAAATAATGTTTAATTCACTGATTCGGCAATGTGTTTTTCTCGTTTTAACGTCAATTATCCTCACATTGATAAGTTACGTTATTTTAATGCAGGATCCATTAAATGCCGAACTGGCGACACCGTATTTTTACAATGGCTACCtt                                                                                                >  1:289363/1‑217 (MQ=255)                                                                                                                                                                                         aaaTATCGACATGAGTCCGTTCGGTAGCCTGAACTTTTCAACCCTCTCCCTGCGAAAAGGGCACAAATAATGTTTAATTCACTGATTCGGCAATGTGTTTTTCTCGTTTTAACGTCAATTATCCTCACATTGATAAGTTACGTTATTTTAATGCAGGATCCATTAAATGCCGAACTGGCGACACCGTATTTTTACAATGGCTACCTTAATTATTTAGGCAACCTTACCCGTGGTGATTTAGGTATCAGTTa                                                    >  1:371731/1‑251 (MQ=255)                                                                                                                                                                                               cGACATGAGTCCGTTCGGTAGCCTGAACTTTTCAACCCTCTCCCTGCGAAAAGGGCACAAATAATGTTTAATTCACTGATTTGGCAATGTGTTTTTCTCGTTTTAACGTCAATTATCCTCACATTGATAAGTTACGTTATTTTAATGCAGGATCCATTAAATGCCGAACTGGCGACACCGTATTTTTACAATGGCTACCTTAATTATTTAGGCAACCTTACCCGTGGTGATTTAGGTATCAGTTACAATgg                                              <  1:448231/251‑1 (MQ=255)                                                                                                                                                                                               cGACATGAGTCCGTTCGGTAGCCTGAACTTTTCAACCCTCTCCCTGCGAAAAGGGCACAAATAATGTTTAATTCACTGATTCGGCAATGTGTTTTTCTCGTTTTAACGTCAATTATCCTCACATTGATAAGTTACGTTATTTTAATGCAGGATCCATTAAATGCCGAACTGGCGACACCGTATTTTTACAATGGCTACCTTAATTATTTAGGCAACCTTACCCGTGGTGATTTAGGTATCAGTTACAATgg                                              <  1:413387/251‑1 (MQ=255)                                                                                                                                                                                                 aCATGAGTCCGTTCGGTAGCCTGAACTTTTCAACCCTCTCCCTGCGAAAAGGGCACAAATAATGTTTAATTCACTGATTCGGCAATGTGTTTTTCTCGTTTTAACGTCAATTATCCTc                                                                                                                                                                                 <  1:683708/118‑1 (MQ=255)                                                                                                                                                                                                 aCATGAGTCCGTTCGGTAGCCTGAACTTTTCAACCCTCTCCCTGCGAAAAGGGCACAAATAATGTTTAATTCACTGATTCGGCAATGTGTTTTTCTCGTTTTAACGTCAATTATCCTCACATTGATAAGTTACGTTATTTTAATGCAGGATCCATTAAATGCCGAACTGGCGACACCGTATTTTTAc                                                                                                            >  1:212521/1‑187 (MQ=255)                                                                                                                                                                                                 aCATGAGTCCGTTCGGTAGCCTGAACTTTTCAACCCTCTCCCTGCGAAAAGGGCACAAATAATGTTTAATTCACTGATTCGGCAATGTGTTTTTCTCGTTTTAACGTCAATTATCCTCACATTGATAAGTTACGTTATTTTAATGCAGGATCCATTAAATGCCGAACTGGCGACACCGTATTTTTACAATGGCTACCTTAATTATTTAGGCAACCTTAccc                                                                          <  1:511074/221‑1 (MQ=255)                                                                                                                                                                                                 aCATGAGTCCGTTCGGTAGCCTGAACTTTTCAACCCTCTCCCTGCGAAAAGGGCACAAATAATGTTTAATTCACTGATTCGGCAATGTGTTTTTCTCGTTTTAACGTCAATTATCCTCACATTGATAAGTTACGTTATTTTAATGCAGGATCCATTAAATGCCGAACTGGCGACACCGTATTTTTACAATGGCTACCTTAATTATTTAGGCAACCTTACCCGTGGTGATTTAGGTATCAGTTACaa                                                 <  1:495794/246‑1 (MQ=255)                                                                                                                                                                                                         ccGTTCGGTAGCCTGAACTTTTCAACCCTCTCCCTGCGAAAAGGGCACAAATAATGTTTAATTCACTGATTCGGCAATGTGTTTTTCTCGTTTTAACGTCAATTATCCTCACATTGATAAGTTACGTTATTTTAATGCAGGATCCATTAAATGCCGAACTGGCGACACCGTATTTTTACAATGGCTACCTTAATTATTTAGGCAACCTTACCCGTGGTGATTTAGGTATCAGTTACAATGGCGGGCAg                                       >  1:260105/1‑248 (MQ=255)                                                                                                                                                                                                           gTTCGGTAGCCTGAACTTTTCACCCCTCTCCCTGCGAAAAGGGCACAAATAATGTTTAATTCACTGATTCGGCAATGTGTTTTtctc                                                                                                                                                                                                      <  1:362284/87‑1 (MQ=255)                                                                                                                                                                                                                    ccTGAACTTTTCAACCCTCTCCCTGCGAAAAGGGCACAAATAATGTTTAATTCACTGATTCGGCAATGTGTTTTTCTCGTTTTAACGTCAATTATCCTCACATTGATAAGTTACGTTATTTTAATGCAGGATCCATTAAATGCCGAACTGGCGACACCGTATTTTTACAATGGCTACCTTAATTATTTAGGCAACCTTACCCGTGGTGATTTAGGTATCAGTTACAATGGCGGGCAGCTGTTAAAATCCAc                         <  1:517841/251‑1 (MQ=255)                                                                                                                                                                                                                            tttCAACCCTCTCCCTGCGAAAAGGGCACAAATAATGTTTAATTCACTGATTCGGCAATGTGTTTTTCTCGTTTTAACGTCAATTATCCTCACATTGATAAGTTACGTTATTTTAATGCAGGATCCAt                                                                                                                                            <  1:101002/128‑1 (MQ=255)                                                                                                                                                                                                                            tttCAACCCTCTCCCTGCGAAAAGGGCACAAATAATGTTTAATTCACTGATTCGGCAATGTGTTTTTCTCGTTTTAACGTCAATTATCCTCACATTGATAAGTTACGTTATTTTAATGCAGGATCCATTAAATGCCGAACTGGCGACACCGTATTTTTACAATGGCTACCTTAATTATTTAGGCAACCTTACCCGTGGTGATTTAgg                                                             >  1:879656/1‑207 (MQ=255)                                                                                                                                                                                                                            tttCAACCCTCTCCCTGCGAAAAGGGCACAAATAATGTTTAATTCACTGATTCGGCAATGTGTTTTTCTCGTTTTAACGTCAATTATCCTCACATTGATAAGTTACGTTATTTTAATGCAGGATCCATTAAATGCCGAACTGGCGACACCGTATTTTTACAATGGCTACCTTAATTATTTAGGCAACCTTACCCGTGGTGATTTAGGTATCAGTTACAATGGCGGGCAGCTGTTAAAATCCACGAtttttt                 >  1:559132/1‑250 (MQ=255)                                                                                                                                                                                                                                        cccTGCGAAAAGGGCACAAATAATGTTTAATTCACTGATTCGGCAATGTGTTTTTCTCGTTTTAACGTCAATTATCCTCACATTGATAAGTTACGTTATTTTAATGCAGGATCCATTAAATGCCGAACTGGCGACACCGTATTTTTACAATGGCTACCTTAATTATTTAGGCAACCTTACCCGTGGTGATTTAGGTATCAGTTACAATGGCGGGCAGCTGTTAAAATCCACGATTTTTACTGTTTTAcccc     <  1:393136/251‑1 (MQ=255)                                                                                                                                                                                                                                            gCGAAAAGGGCACAAATAATGTTTAATTCACTGATTCGGCAATGTGTTTTTCTCGTTTTAACGTCAATTATCCTCACATTGATAAGTTACGTTATTTTAATGCAGGATCCATTAAATGCCGAACTGGCGACACCGTATTTTTACAATGGCTACCTTAATTATTTAGGCAACCTTAccc                                                                          >  1:313372/1‑178 (MQ=255)                                                                                                                                                                                                                                            gCGAAAAGGGCACAAATAATGTTTAATTCACTGATTCGGCAATGTGTTTTTCTCGTTTTAACGTCAATTATCCTCACATTGATAAGTTACGTTATTTTAATGCAGGATCCATTAAATGCCGAACTGGCGACACCGTATTTTTACAATGGCTACCTTAATTATTTAGGCAACCTTACCCGTGGTGATTTAGGTATCAGTTACAATGGCGGGCAGCTGTTAAAATCCACGATTTTTACTGTTTTACCCCCCa  >  1:114890/1‑250 (MQ=255)                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                 TTACCAACTTATCCAACTGGTGCAGCGAAAAATTTGATCAAATTATGGATGAGGCATTGGATACTTCCAATCAGCGCGTACGCTCCGCTGCTTATAATCAGGCACAGACGTTAATTTTATCGGAGTTACCAATCATTCCGATCGCGAACGTTAAGCGGGTTCTCGTCGCCAGTTCGCGTGTTCAAAATATCGACATGAGTCCGTTCGGTAGCCTGAACTTTTCAACCCTCTCCCTGCGAAAAGGACACAAATAATGTTTAATTCACTGATTCGGCAATGTGTTTTTCTCGTTTTAACGTCAATTATCCTCACATTGATAAGTTACGTTATTTTAATGCAGGATCCATTAAATGCCGAACTGGCGACACCGTATTTTTACAATGGCTACCTTAATTATTTAGGCAACCTTACCCGTGGTGATTTAGGTATCAGTTACAATGGCGGGCAGCTGTTAAAATCCACGATTTTTACTGTTTTACCCCCCA  >  1/1659886‑1660370

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑