Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,655,423	C→T	intergenic (+13584/+8952)	unknown → / ← unknown	tRNA‑Gly‑GCC/LSU rRNA ## 23S rRNA, large subunit ribosomal RNA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,655,423	0	C	T	100.0%	195.9 / NA	69	intergenic (+13584/+8952)	unknown/unknown	tRNA‑Gly‑GCC/LSU rRNA ## 23S rRNA, large subunit ribosomal RNA
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (29/40);  total (29/40)

ACATAATGCTGTAACGGCTGCTGCGCCGTGCAAATCACACGTACATTGGCATAATGCTCCTCTTCCTCTCCGACACGGCGGAAAGTACCGTCGCTTAAAAAACGCAGAAGCTTCGCCTGCAAAGTCAGAGGCAACTCCTCAACACCATCTAACAACACCGTGCCGCCATCGGCATATTCAAAAAAACCGATAAATTCACCATCTTTATTACGCCGCCCGAACATTTCCGTTTCCGCGTCCTCGCCGGGAATGCCGGCACAGTTTACGGCGAGAAATGCTTTATTTCGGCGCGCACTAAAATCATGGCAGGCTTTGGCAATCACGTCTTTTCCCGTGCCGGTTTCACCTTGAATCACCAACGGCGCATCCAATAACGCAAATTTTCTGGCTTTCGCAATAACATCCCGCATTCGCGGATTTCGGCTGACGATGTGTGAAAAAGGATCGGTGGTTGAAGAAGTTGCCATAAGTGCCTCAATAA  >  1/1655184‑1655664                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                  aCATAATGCTGTAACGGCTGCTGCGCCGTGCAAATCACACGTACATTGGCATAATGCTCCTCTTCCTCTCCGACACGGCGGAAAGTACCGTCGCTTAAAAAACGCAGAAGCTTCGCCTGCAAAGTCAGAGGCAACTCCTCAACACCATCTAACAACACCGTGCCGCCATCGGCATATTCAAAAAAACCGATAAATTCACCATCTTTATTACGCCGCCCGAACATTTCCGTTTCCGCGTCTTCGCCGGGAAt                                                                                                                                                                                                                                        >  1:496765/1‑251 (MQ=255) aCATAATGCTGTAACGGCTGCTGCGCCGTGCAAATCACACGTACATTGGCATAATGCTCCTCTTCCTCTCCGACACGGCGGAAAGTACCGTCGCTTAAAAAACGCAGAAGCTTCGCCTGCAAAGTCAGAGGCAACTCCTCAACACCATCTAACAACACCGTGCCGCCATCGGCATATTCAAAAAAACCGATAAATTCACCATCTTTATTACGCCGCCCGAACATTTCCGTTTCCGCGTCTTCGCCGGGAAt                                                                                                                                                                                                                                        >  1:462010/1‑251 (MQ=255)     aaTGCTGTAACGGCTGCTGCGCCGTGCAAATCACACGTACATTGGCATAATGCTCCTCTTCCTCTCCGACACGGCGGAAAGTACCGTCGCTTAAAAAACGCAGAAGCTTCGCCTGCAAAGTCAGAGGCAACTCCTCAACACCATCTAACAACACCGTGCCGCCATCGGCATATTCAAAAAAACCGATAAATTCACCATCTTTATTACGCCGCCCGAACATTTCCGTTTCCGCGTCTTCGCCGGGAATGCCg                                                                                                                                                                                                                                    <  1:188031/251‑1 (MQ=255)      aTGCTGTAACGGCTGCTGCGCCGTGCAAATCACACGTACATTGGCATAATGCTCCTCATCCTCTCCGACACGGCGGAAAGTACCGTCGCTTAAAAAACGCAGAAGCTTCGCCTGCAAAGTCAGAGGCAACTCCTCAACACCATCTAACAACACCGTGCCGCCATCGGCATATTCAAAAAAACCGATAAATTCACCATCTTTATTACGCCGCCCGAACATTTCCGTTTCCGCGTCTTCGCCGGGAATGCCgg                                                                                                                                                                                                                                   <  1:378434/251‑1 (MQ=255)         cTGTAACGGCTGCTGCGCCGTGCAAATCACACGTACATTGGCATAATGCTCCTCTTCCTCTCCGACACGGCGGAAAGTACCGTCGCTTAAAAAACGCAGAAGCTTCGCCTGCAAAGTCAGAGGCAACTCCTCAACACCATCTAACAACACCGTGCCGCCATCGGCATATTCAAAAAAACCGATAAATTCACCATCTTTATTACGCCGCCCGAACATTTCCGTTTCCGCGTCTTCGCCggg                                                                                                                                                                                                                                           >  1:884947/1‑240 (MQ=255)         cTGTAACGGCTGCTGCGCCGTGCAAATCACACGTACATTGGCATAATGCTCCTCTTCCTCTCCGACACGGCGGAAAGTACCGTCGCTTAAAAAACGCAGAAGCTTCGCCTGCAAAGTCAGAGGCAACTCCTCAACACCATCTAACAACACCGTGCCGCCATCGGCATATTCAAAAAAACCGATAAATTCACCATCTTTATTACGCCGCCCGAACATTTCCGTTTCCGCGTCTTCGCCGGGAATGCCGGcac                                                                                                                                                                                                                                <  1:323699/251‑1 (MQ=255)               cGGCTGCTGCGCCGTGCAAATCACACGTACATTGGCATAATGCTCCTCTTCCTCTCCGACACGGCGGAAAGTACCGTCGCTTAAAAAACGCAGAAGCTTCGCCTGCAAAGTCAGAGGCAACTCCTCAACACCATCTAACAACACCGTGCCGCCATCGGCATATTCAAAAAAACCGATAAATTCACCATCTTTATTACGCCGCCCGAACATTTCCGTTTCCGCGTCTTCGCCGGGAATGCCGGCACAGttt                                                                                                                                                                                                                           <  1:50560/250‑1 (MQ=255)                  ctgctgCGCCGTGCAAATCACACGTACATTGGCATAATGCTCCTCTTCCTCTCCGACACGGCGGAAAGTACCGTCGCTTAAAAAACGCAGAAGCTTCGCCTGCAAAGTCAGAGGCAACTCCTCAACACCATCTAACAACACCGTGCCGCCATCGGCATATTCAAAAAAACCGATAAATTCACCATCTTTATTACGCCGCCCGAACATTTCCGTTTCCGCGTCTTCGCCGGGAATGCCGGCACAGTTTACgg                                                                                                                                                                                                                       >  1:26106/1‑251 (MQ=255)                   tgctgcGCCGTGCAAATCACACGTACATTGGCATAATGCTCCTCTTCCTCTCCGACACGGCGGAAAGTACCGTCGCTTAAAAAACGCAGAAGCTTCGCCTGCAAAGTCAGAGGCAACTCCTCAACACCATCTAACAACACCGTGCCGCCATCGGCATATTCAAAAAAACCGATAAATTCACCATCTTTATTACGCCGCCCGAACATTTCCGTTTCCGCGTCTTCGCCGGGAATGCCGGCACAGTTTACGGc                                                                                                                                                                                                                      <  1:589633/251‑1 (MQ=255)                                   tCACACGTACATTGGCATAATGCTCCTCTTCCTCTCCGACACGGCGGAAAGTACCGTCGCTTAAAAAACGCAGAAGCTTCGCCTGCAAAGTCAGAGGCAACTCCTCAACACCATCTAACAACACCGTGCCGCCATCGGCATATTCAAAAAAACCGATAAATTCACCATCTTTATTACGCCGCCCGAACATTTCCGTTTCCGCGTCTTCGCCGGGAATGCCGGcac                                                                                                                                                                                                                                <  1:273343/225‑1 (MQ=255)                                          tACATTGGCATAATGCTCCTCTTCCTCTCCGACACGGCGGAAAGTACCGTCGCTTAAAAAACGCAGAAGCTTCGCCTGCAAAGTCAGAGGCAACTCCTCAACACCATCTAACAACACCGTGCCGCCATCGGCATATTCAAAAAAACCGATAAATTCACCATCTTTATTACGCCGCCCGAACATTTCCGTTTCCGCGTCTTCGCCGGGAATGCCGGCACAGTTTACGGCGAGAAATGCTTTATTTCGgcgcg                                                                                                                                                                                               >  1:181382/1‑251 (MQ=255)                                          tACATTGGCATAATGCTCCTCTTCCTCTCCGACACGGCGGAAAGTACCGTCGCTTAAAAAACGCAGAAGCTTCGCCTGCAAAGTCAGAGGCAACTCCTCAACACCATCTAACAACACCGTGCCGCCATCGGCATATTCAAAAAAACCGATAAATCCACCATCTTTATTACGCCGCCCGAACATTTCCGTTTCCGCGTCTTCGCCGGGAATGCCGGCACAGTTTACGGCGAGAAATGCTTTATTTCGgcgcg                                                                                                                                                                                               >  1:744221/1‑251 (MQ=255)                                            cATTGGCATAATGCTCCTCTTCCTCTCCGACACGGCGGAAAGTACCGTCGCTTAAAAAACGCAGAAGCTTCGCCTGCAAAGTCAGAGGCAACTCCTCAACACCATCTAACAACACCGTGCCGCCATCGGCATATTCAAAAAAACCGATAAATTCACCATCTTTATTACGCCGCCCGAACATTTCCGTTTCCGCGTCTTCGCCGGGAATGCCGGCACAGTTTACGGc                                                                                                                                                                                                                      >  1:29505/1‑226 (MQ=255)                                                 gCATAATGCTCCTCTTCCTCTCCGACACGGCGGAAAGTACCGTCGCTTAAAAAACGCAGAAGCTTCGCCTGCAAAGTCAGAGGCAACTCCTCAACACCATCTAACAACACCGTGCCGCCATCGGCATATTCAAAAAAACCAATAAATTCACCATCTTTATTACGCCGCCCGAACATTTCCGTTTCCGCGTCTTCGCCGGGAATGCCGGCACAGTTTACGGCGAGAAATGCTTTAttt                                                                                                                                                                                                      <  1:826895/237‑1 (MQ=255)                                                   aTAATGCTCCTCTTCCTCTCCGACACGGCGGAAAGTACCGTCGCTTAAAAAACGCAGAAGCTTCGCCTGCAAAGTCAGAGGCAACTCCTCAACACCATCTAACAACACCGTGCCGCCATCGGCATATTCAAAAAAACCGATAAATTCACCATCTTTATTACGCCGCCCGAACATTTCCGTTTCCGCGTCTTCGCCGGGAATGCCGGCACAGTTTACGGCGAGAAATGCTTTATTTCGGCGTGCACTAAAAt                                                                                                                                                                                      <  1:640540/251‑1 (MQ=255)                                                   aTAATGCTCCTCTTCCTCTCCGACACGGCGGAAAGTACCGTCGCTTAAAAAACGCAGAAGCTTCGCCTGCAAAGTCAGAGGCAACTCCTCAACACCATCTAACAACACCGTGCCGCCATCGGCATATTCAAAAAAACCGATAAATTCACCATCTTTATTACGCCGCCCGAACATTTCCGTTTCCGCGTCTTCGCCGGGAATGCCGGCACAGTTTACGGCGAGAAATGCTTTATTTCGGCGCGCACTAAAAt                                                                                                                                                                                      <  1:196104/251‑1 (MQ=255)                                                      aTGCTCCTCTTCCTCTCCGACACGGCGGAAAGTACCGTCGCTTAAAAAACGCAGAAGCTTCGCCTGCAAAGTCAGAGGCAACTCCTCAACACCATCTAACAACACCGTGCCGCCATCGGCATATTCAAAAAAACCGATAAATTCACCATCTTTATTACGCCGCCCGAACATTTCCGTTTCCGCGTCTTCGCCGGGAATGCCGGCACAGttt                                                                                                                                                                                                                           <  1:838197/211‑1 (MQ=255)                                                           cctcttcctctCCGACACGGCGGAAAGTACCGTCGCTTAAAAAACGCAGAAGCTTCGCCTGCAAAGTCAGAGGCAACTCCTCAACACCATCTAACAACACCGTGCCGCCATCGGCATATTCAAAAAAACCGATAAATTCACCATCTTTATTACGCCGCCCGAACATTTCCGTTTCCGCGTCTTCGCCGGGAATGCCGGCACAGTTTACGGCGAGAAATGCTTTATTTCGGCGCGCACTaaaa                                                                                                                                                                                       >  1:734795/1‑242 (MQ=255)                                                           cctcttcctctCCGACACGGCGGAAAGTACCGTCGCTTAAAAAACGCAGAAGCTTCGCCTGCAAAGTCAGAGGCAACTCCTCAACACCATCTAACAACACCGTGCCGCCATCGGCATATTCAAAAAAACCGATAAATTCACCATCTTTATTACGCCGCCCGAACATTTCCGTTTCCGCGTCTTCGCCGGGAATGCCGGCACAGTTTACGGCGAGAAATGCTTTATTTCGGCGCGCACTAAAATCATGGCAg                                                                                                                                                                              >  1:234806/1‑251 (MQ=255)                                                             tcttcctctCCGACACGGCGGAAAGTACCGTCGCTTAAAAAACGCAGAAGCTTCGCCTGCAAAGTCAGAGGCAACTCCTCAACACCATCTAACAACACCGTGCCGCCATCGGCATATTCAAAAAAACCGATAAATTCACCATCTTTATTACGCCGCCCGAACATTTCCGTTTCCGCGTCTTCGCCGGGAATGCCGGCACAGTTTACGGCGAGAAATGCTTTATTTCGGCGCGCACTAAAATCATGGCAGGc                                                                                                                                                                            <  1:446653/251‑1 (MQ=255)                                                                  ctctCCGACACGGCGGAAAGTACCGTCGCTTAAAAAACGCAGAAGCTTCGCCTGCAAAGTCAGAGGCAACTCCTCAACACCATCTAACAACACCGTGCCGCCATCGGCATATTCAAAAAAACCGATAAATTCACCATCTTTATTACGCCGCCCGAACATTTCCGTTTCCGCGTCTTCGCCGGGAATGCCGGCACAGTTTACGGCGAGAAATGCTTTATTTCGGCGCGCACTAAAATCATGGCAGGCGTTgg                                                                                                                                                                       >  1:46989/1‑251 (MQ=255)                                                                   tctcCGACACGGCGGAAAGTACCGTCGCTTAAAAAACGCAGAAGCTTCGCCTGCAAAGTCAGAGGCAACTCCTCAACACCATCTAACAACACCGTGCCGCCATCGGCATATTCAAAAAAACCGATAAATTCACCATCTTTATTACGCCGCCCGAACATTTCCGTTTCCGCGTCTTCGCCGGGAATGCCGGCACAGTTTACGGCGAGAAATGCTTTATTTCGGCGCGCACTAAAATCATGGCAGGCTTTGGc                                                                                                                                                                      <  1:211821/251‑1 (MQ=255)                                                                    ctcCGACACGGCGGAAAGTACCGTCGCTTAAAAAACGCAGAAGCTTCGCCTGCAAAGTCAGAGGCAACTCCTCAACACCATCTAACAACACCGTGCCGCCATCGGCATATTCAAAAAAACCGATAAATTCACCATCTTTATTACGCCGCCCGAACATTTCCGTTTCCGCGTCTTCGCCGGGAATGCCGGCACAGTTTACGGCGAGAAATGCTTTATTTCGGCGCGCACTAAAATCATGGCAGGCTTTGGCa                                                                                                                                                                     >  1:237125/1‑251 (MQ=255)                                                                              gcggAAAGTACCGTCGCTTAAAAAACGCAGAAGCTTCGCCTGCAAAGTCAGAGGCAACTCCTCAACACCATCTAACAACACCGTGCCGCCATCGGCATATTCAAAAAAACCGATAAATTCACCATCTTTATTACGCCGCCCGAACATTTCCGTTTCCGCGTCTTCGCCGGGAATGCCGGCACAGTTTACGGCGAGAAATGCTTTATTTCGGCGCGCACTAAAATCATGGCAGGCTTTGGCAATCACGTCt                                                                                                                                                            <  1:42230/250‑1 (MQ=255)                                                                                ggAAAGTACCGTCGCTTAAAAAACGCAGAAGCTTCGCCTGCAAAGTCAGAGGCAACTCCTCAACACCATCTAACAACACCGTGCCGCCATCGGCATATTCAAAAAAACCGATAAATTCACCATCTTTATTACGCCGCCCGAACATTTCCGTTTCCGCGTCTTCGCCGGGAATGCCGGCACAGTTTACGGCGAGAAATGCTTTATTTCGGCGCGCACTAAAATCATGGCAGGCTTTGGc                                                                                                                                                                      <  1:529709/238‑1 (MQ=255)                                                                                  aaaGTACCGTCGCTTAAAAAACGCAGAAGCTTCGCCTGCAAAGTCAGAGGCAACTCCTCAACACCATCTAACAACACCGTGCCGCCATCGGCATATTCAAAAAAACCGATAAATTCACCATCTTTATTACGCCGCCCGAACATTTCCGTTTCCGCGTCTTCGCCGGGAATGCCGGCACAGTTTACGGCGAGAAATGCTTTATTTCGGCGCGCACTAAAATCATGGCAGGCTTTGGCAATCACGTCTTTTcc                                                                                                                                                       <  1:351474/251‑1 (MQ=255)                                                                                                            aaGCTTCGCCTGCAAAGTCAGAGGCAACTCCTCAACACCATCTAACAACACCGTGCCGCCATCGGCATATTCAAAAAAACCGATAAATTCACCATCTTTATTACGCCGCCCGAACATTTCCGTTTCCGCGTCTTCGCCGGGAATGCCGGCACAGTTTACGGCGAGAAATGCTTTATTTCGGCGCGCACTAAAATCATGGCAGGCTTTGGCAATCACGTCTTTTCCCGTGCCGGTTTCACCTTGAATCAcc                                                                                                                              <  1:387428/250‑1 (MQ=255)                                                                                                            aaGCTTCGCCTGCAAAGTCAGAGGCAACTCCTCAACACCATCTAACAACACCGTGCCGCCATCGGCATATTCAAAAAAACCGATAAATTCACCATCTTTATTACGCCGCCCGAACATTTCCGTTTCCGCGTCTTCGCCGGGAATGCCGGCACAGTTTACGGCGAGAAATGCTTTATTTCGGCGCGCACTAAAATCATGGCAGGCTTTGGCAATCACGTCTTTTCCCGTGCCGGTTTCACCTTGAATCACCa                                                                                                                             <  1:789145/251‑1 (MQ=255)                                                                                                             aGCTTCGCCTGCAAAGTCAGAGGCAACTCCTCAACACCATCTAACAACACCGTGCCGCCATCGGCATATTCAAAAAAACCGATAAATTCACCATCTTTATTACGCCGCCCGAACATTTCCGTTTCCGCGTCTTCGCCGGGAATGCCGGCACAGTTTACGGCGAGAAATGCTTTATTTCGGCGCGCACTAAAATCATGGCAGGCTTTGGCAATCACGTCTTTTCCCGTGCCGGTTTCACCTTGAATc                                                                                                                                 <  1:133470/246‑1 (MQ=255)                                                                                                             aGCTTCGCCTGCAAAGTCAGAGGCAACTCCTCAACACCATCTAACAACACCGTGCCGCCATCGGCATATTCAAAAAAACCGATAAATTCACCATCTTTATTACGCCGCCCGAACATTTCCGTTTCCGCGTCTTCGCCGGGAATGCCGGCACAGTTTACGGCGAGAAATGCTTTATTTCGGCGCGCACTAAAATCATGGCAGGCTTTGGCAATCACGTCTTTTCCCGTGCCGGTTTCACCTTGAATc                                                                                                                                 <  1:83815/246‑1 (MQ=255)                                                                                                                  cGCCTGCAAAGTCAGAGGCAACTCCTCAACACCATCTAACAACACCGTGCCGCCATCGGCATATTCAAAAAAACCGATAAATTCACCATCTTTATTACGCCGCCCGAACATTTCCGTTTCCGCGTCTTCGCCGGGAATGCCGGCACAGTTTACGGCGAGAAATGCTTTATTTCGGCGCGCACTAAAATCATGGCAGGCTTTGGCAATCACGTCTTTTCCCGTGCCGGTTTCACCTTGAATCACCAACGgcg                                                                                                                       <  1:524759/251‑1 (MQ=255)                                                                                                                        cAAAGTCAGAGGCAACTCCTCAACACCATCTAACAACACCGTGCCGCCATCGGCATATTCAAAAAAACCGATAAATTCACCATCTTTATTACGCCGCCCGAACATTTCCGTTTCCGCGTCTTCGCCGGGAATGCCGGCACAGTTTACGGCGAGAAATGCTTTATTTCGGCGCGCACTAAAATCATGGCAGGCTTTGGCAATCACGTCTTTTCCCGTGCCGGTTTCACCTTGAATCACCAACGGCGCATCCa                                                                                                                 <  1:606767/251‑1 (MQ=255)                                                                                                                            gTCAGAGGCAACTCCTCAACACCATCTAACAACACCGTGCCGCCATCGGCATATTCAAAAAAACCGATAAATTCACCATCTTTATTACGCCGCCCGAACATTTCCGTTTCCGCGTCTTCGCCGGGAATGCCGGCACAGTTTACGGCGAGAAATGCTTTATTTCGGCGCGCACTAAAATCATGGCAGGCTTTGGCAATCACGTCTTTTCCCGTGCCGGTTTCACCTTGAATCACCAACGGCGCATCCAATaa                                                                                                             >  1:714812/1‑251 (MQ=255)                                                                                                                             tCAGAGGCAACTCCTCAACACCATCTAACAACACCGTGCCGCCATCGGCATATTCAAAAAAACCGATAAATTCACCATCTTTATTACGCCGCCCGAACATTTCCGTTTCCGCGTCTTCGCCGGGAATGCCGGCACAGTTTACGGCGAGAAATGCTTTATTTCGGCGCGCGCTAAAATCATGGCAGGCTTTGGCAATCACGTCTTTTCCCGTGCCGGTTTCACCTTGAATCACCAACGGCGCATCCAATAAc                                                                                                            <  1:314756/251‑1 (MQ=255)                                                                                                                                       ctcctcAACACCATCTAACAACACCGTGCCGCCATCGGCATATTCAAAAAAACCGATAAATTCACCATCTTTATTACGCCGCCCGAACATTTCCGTTTCCGCGTCTTCGCCGGGAATGCCGGCACAGTTTACGGCGAGAAATGCTTTATTTCGGCGCGCACTAAAAt                                                                                                                                                                                      >  1:347344/1‑167 (MQ=255)                                                                                                                                         cctcAACACCATCTAACAACACCGTGCCGCCATCGGCATATTCAAAAAAACCGATAAATTCACCATCTTTATTACGCCGCCCGAACATTTCCGTTTCCGCGTCTTCGCCGGGAATGCCGGCACAGTTTACGTCGAGAAATGCTTTATTTCGGCGCGCACTAAAATCATGGCAGGCTTTGGCAATCACGTCTTTTCCCGTGCCGGTTTCACCTTGAATCACCAACGGCGCATCCAATAACGCAAAtttt                                                                                                   >  1:483111/1‑248 (MQ=255)                                                                                                                                         cctcAACACCATCTAACAACACCGTGCCGCCATCGGCATATTCAAAAAAACCGATAAATTCACCATCTTTATTACGCCGCCCGAACATTTCCGTTTCCGCGTCTTCGCCGGGAATGCCGGCACAGTTTACGGCGAGAAATGCTTTATTTCGGCGCGCACTAAAATCATGGCAGGCTTTGGCAATCACGTCTTTTcc                                                                                                                                                       >  1:121670/1‑196 (MQ=255)                                                                                                                                         cctcAACACCATCTAACAACACCGTGCCGCCATCGGCATATTCAAAAAAACCGATAAATTCACCATCTTTATTACGCCGCCCGAACATTTCCGTTTCCGCGTCTTCGCCGGGAATGCCGGCACAGTTTACGGCGAGAAATGCTTTATTTCGGCGCGCACTAAAATCATGGCAGGCTTTGGCAATCACGTCTTTTCCCGTGCCGGTTTCACCTTGAATCACCAACGGCGCATCCAATAACGCAAAtttt                                                                                                   >  1:759537/1‑248 (MQ=255)                                                                                                                                         cctcAACACCATCTAACAACACCGTGCCGCCATCGGCATATTCAAAAAAACCGATAAATTCACCATCTTTATTACGCCGCCCGAACATTTCCGTTTCCGCGTCTTCGCCGGGAATGCCGGCACAGTTTACGGCGAGAAATGCTTTATTTCGGCGCGCACTAAAATCATGGCAGGCTTTGGCAATCACGTCTTTTCCCGTGCCGGTTTCACCTTGAATCACCAACGGCGCATCCAATAACGCAAAtttt                                                                                                   >  1:751281/1‑248 (MQ=255)                                                                                                                                                    tCTAACAACACCGTGCCGCCATCGGCATATTCAAAAAAACCGATAAATTCACCATCTTTATTACGCCGCCCGAACATTTCCGTTTCCGCGTCTTCGCCGGGAATGCCGGCACAGTTTACgg                                                                                                                                                                                                                       <  1:772027/121‑1 (MQ=255)                                                                                                                                                    tCTAACAACACCGTGCCGCCATCGGCATATTCAAAAAAACCGATAAATTCACCATCTTTATTACGCCGCCCGAACATTTCCGTTTCCGCGTCTTCGCCGGGAATGCCGGCACAGTTTACGGCGAGAAATGCTTTATTTCGGCGCGCACTAAAATCATGGCAGGCTTTGGCAATCACGTCTTTTCCCGTGCCGGTTTCACCTTGAATCACCAACGGCGCATCCAATAACGCAAATTTTCTGGCTTTCGCAAt                                                                                     >  1:831619/1‑251 (MQ=255)                                                                                                                                                     cTAACAACACCGTGCCGCCATCGGCATATTCAAAAAAACCGATAAATTCACCATCTTTATTACGCCGCCCGAACATTTCCGTTTCCGCGTCTTCGCCGGGAATGCCGGCACAGTTTACGGCGAGAAATGCTTTATTTCGGCGCGCACTAAAATCATGGCAGGCTTTGGCAATCACGTCTTTTCCCGTGCCGGTTTCACCTTGAATCACCAACgg                                                                                                                         >  1:863589/1‑214 (MQ=255)                                                                                                                                                     cTAACAACACCGTGCCGCCATCGGCATATTCAAAAAAACCGATAAATTCACCATCTTTATTACGCCGCCCGAACATTTCCGTTTCCGCGTCTTCGCCGGGAATGCCGGCACAGTTTACGGCGAGAAATGCTTTATTTCGGCGCGCACTAAAATCATGGCAGGCTTTGGCAATCACGTCTTTTCCCGTGCCGGTTTCACCTTGAATCACCAACGGCGCATCCAATAACGCAAATTTTCTGGCTTTCGCAATa                                                                                    >  1:508663/1‑251 (MQ=255)                                                                                                                                                      tAACAACACCGTGCCGCCATCGGCATATTCAAAAAAACCGATAAATTCACCATCTTTATTACGCCGCCCGAACATTTCCGTTTCCGCGTCTTCGCCGGGAATGCCGGCACAGTTTACGGCGAGAAATGCTTTATTTCGGCGCGCACTAAAATCATGGCAGGCTTTGGc                                                                                                                                                                      >  1:847166/1‑168 (MQ=255)                                                                                                                                                       aacaacACCGTGCCGCCATCGGCATATTCAAAAAAACCGATAAATTCACCATCTTTATTACGCCGCCCGAACATTTCCGTTTCCGCGTCTTCGCCGGGAATGCCGGCACAGTTTACGGCGAGAAATGCTTTATTTCGGCGCGCACTAAAATc                                                                                                                                                                                     <  1:814615/152‑1 (MQ=255)                                                                                                                                                       aacaacACCGTGCCGCCATCGGCATATTCAAAAAAACCGATAAATTCACCATCTTTATTACGCCGCCCGAACATTTCCGTTTCCGCGTCTTCGCCGGGAATGCCGGCACAGTTTACGGCGAGAAATGCTTTATTTCGGCGCGCACTAAAATCATGGCAGGCTTTGGCAATCACGTCTTTTCCCGTGCCGGTTTCACCTTGAATCACCAACGGCGCATCCAATAACGCAAATTTTCTGGCTTTCGCAATaa                                                                                   >  1:812201/1‑250 (MQ=255)                                                                                                                                                       aacaacACCGTGCCGCCATCGGCATATTCAAAAAAACCGATAAATTCACCATCTTTATTACGCCGCCCGAACATTTCCGTTTCCGCGTCTTCGCCGGGAATGCCGGCACAGTTTACGGCGAGAAATGCTTTATTTCGGCGCGCACTAAAATCATGGCAGGCTTTGGCAATCACGTCTTTTCCCGTGCCGGTTTCACCTTGAATCACCAACGGCGCATCCAATAACGCAAATTTTCTGGCTTTCGCAATAAc                                                                                  <  1:702109/251‑1 (MQ=255)                                                                                                                                                         caacaCCGTGCCGCCATCGGCATATTCAAAAAAACCGATAAATTCACCATCTTTATTACGCCGCCCGAACATTTCCGTTTCCGCGTCTTCGCCGGGAATGCCGGCACAGTTTACGGCGAGAAATGCTTTATTTCGGCGCGCACTAAAATCATGGCAGGCTTTGGCAATCACGTCTTTTCCCGTGCCGGTTTCACCTTGAATCACCAACGGCGCATCCAATAACGCAAATTTTCTGGCTTTCGCAATAACAt                                                                                <  1:230715/251‑1 (MQ=255)                                                                                                                                                                gTGCCGCCATCGGCATATTCAAAAAAACCGATAAATTCACCATCTTTATTACGCCGCCCGAACATTTCCGTTTCCGCGTCTTCGCCGGGAATGCCGGCACAGTTTACGGCGAGAAATGCTTTATTTCGGCGCGCACTAAAATCATGGCAGGCTTTGGCAATCACGTCTTTTccc                                                                                                                                                      <  1:505379/174‑1 (MQ=255)                                                                                                                                                                  gccgccATCGGCATATTCAAAAAAACCGATAAATTCACCATCTTTATTACGCCGCCCGAACATTTCCGTTTCCGCGTCTTCGCCGGGAATGCCGGCACAGTTTACGGCGAGAAATGCTTTATTTCGGCGCGCACTAAAATCATGGCAGGCTTTGGCAATCACGTCTTTTCCCGTGCCGGTTTCACCTTGAATCACCAACGGCGCATCCAATAACGCAAATTTTCTGGCTTTCGCAATAACATCCCGCAtt                                                                        >  1:308643/1‑250 (MQ=255)                                                                                                                                                                             cATATTCAAAAAAACCGATAAATTCACCATCTTTATTACGCCGCCCGAACATTTCCGTTTCCGCGTCTTCGCCGGGAATGCCGGCACAGTTTACGGCGAGAAATGCTTTATTTCGGCGCGCACTAAAATCATGGCAGGCTTTGGCAATCACGTCTTTTCCCGTGCCGGTTTCTCCTTGAATCACCAACGGCGCATCCAATAACGCAAATTTTCTGGCTTTCGCAATAACATCCCGCATTCGCGGATTTCg                                                             <  1:43367/250‑1 (MQ=255)                                                                                                                                                                                           ccGATAAATTCACCATCTTTATTACGCCGCCCGAACATTTCCGTTTCCGCGTCTTCGCCggg                                                                                                                                                                                                                                           >  1:108358/1‑62 (MQ=255)                                                                                                                                                                                            cGATAAATTCACCATCTTTATTACGCCGCCCGAACATTTCCGTTTCCGCGTCTTCGCCGGGAATGCCGGCACAGTTTACGGCGAGAAATGCTTTATTTCGGCGCGCACTAAAATCATGGCAGGCTTTGGCAATCACGTCTTTTCCCGTGCCGGTTTCACCTTGAATCACCAACGGCGCATCCAATAACGCAAATTTTCTGGCTTTCGCAATAACATCCCGCATTCGCGGATTTCGGCTGACGATGTGTGaa                                             <  1:357744/251‑1 (MQ=255)                                                                                                                                                                                                 aaTTCACCATCTTTATTACGCCGCCCGAACATTTCCGTTTCCGCGTCTTCGCCGGGAATGCCGGCACAGTTTACGGCGAGAAATGCTTTATTTCGGCGCGCACTAAAATCATGGCAGGCTTTGGCAATCACGTCTTTTCCCGTGCCGGTTTCACCTTGAATCACCAACGGCGCATCCAATAACGCAAATTTTCTGGCTTTCGCAATAACATCCCGCATTCGCGGATTTCGGCTGACGATGTGTGAAAAAgg                                        <  1:308374/251‑1 (MQ=255)                                                                                                                                                                                                          tCTTTATTACGCCGCCCGAACATTTCCGTTTCCGCGTCTTCGCCGGGAATGCCGGCACAGTTTACGGCGAGAAATGCTTTATTTCGGCGCGCACTAAAATCATGGCAGGCTTTGGCAATCACGTCTTTTCCCGTGCCGGTTTCACCTTGAATCACCAACGGCGCATCCAATAACGCAAATTTTCTGGCTTTCGCAATAACATCCCGCATTCGCGGATTTCGGCTGACGATGTGTGAAAAAGGATCggtggt                               >  1:252680/1‑251 (MQ=255)                                                                                                                                                                                                          tCTTTATTACGCCGCCCGAACATTTCCGTTTCCGCGTCTTCGCCGGGAATGCCGGCACAGTTTACGGCGAGAAATGCTTTATTTCGGCGCGCACTAAAATCATGGCAGGCTTTGGCAATCACGTCTTTTCCCGTGCCGGTTTCACCTTGAATCACCAACGGCGCATCCAATAACGCAAATTTTCTGGCTTTCGCAATAACATCCCGCATTCGCGGATTTCGGCTGACGATGTGTGAAAAAGGATCggtggt                               >  1:464153/1‑251 (MQ=255)                                                                                                                                                                                                           cTTTATTACGCCGCCCGAACATTTCCGTTTCCGCGTCTTCGCCGGGAATGCCGGCACAGTTTACGGCGAGAAATGCTTTATTTCGGCGCGCACTAAAATCATGGCAGGCTTTGGCAATCACGTCTTTTCCCGTGCCGGTTTCACCTTGAATCAcc                                                                                                                              <  1:497107/155‑1 (MQ=255)                                                                                                                                                                                                           cTTTATTACGCCGCCCGAACATTTCCGTTTCCGCGTCTTCGCCGGGAATGCCGGCACAGTTTACGGCGAGAAATGCTTTATTTCGGCGCGCACTAAAATCATGGCAGGCTTTGGCAATCACGTCTTTTCCCGTGCCGGTTTCACCTTGAATCACCAACGGCGCATCCAATAACGCAAATTTTCTGGCTTTCGCAATAACATCCCGCATTCGCGGATTTCGGCTGACGATGTGTGAAAAAGGATCGGTGGtt                              >  1:816309/1‑251 (MQ=255)                                                                                                                                                                                                           cTTTATTACGCCGCCCGAACATTTCCGTTTCCGCGTCTTCGCCGGGAATGCCGGCACAGTTTACGGCGAGAAATGCTTTATTTCGGCGCGCACTAAAATCATGGCAGGCTTTGGCAATCACGTCTTTTCCCGTGCCGGTTTCACCTTGAATCACCAACGGCGCATCCAATAACGCAAATTTTCTGGCTTTCGCAATAACATCCCGCATTCGCGGATTTCGGCTGACGATGTGTGAAAAAGGATCGGTGGtt                              >  1:88704/1‑251 (MQ=255)                                                                                                                                                                                                             ttattaCGCCGCCCGAACATTTCCGTTTCCGCGTCTTCGCCGGGAATGCCGGCACAGTTTACGGCGAGAAATGCTTTATTTCGGCGCGCACTAAAATCATGGCAGGCTTTGGCAATCACGTCTTTTCCCGTGCCGGTTTCACCTTGAATCGCCAACGGCGCATCCAATAACGCAAATTTTCTGGCTTTCGCAATAACATCCCGCATTCGCGGATTTCGGc                                                           <  1:4449/220‑1 (MQ=255)                                                                                                                                                                                                             ttattaCGCCGCCCGAACATTTCCGTTTCCGCGTCTTCGCCGGGAATGCCGGCACAGTTTACGGCGAGAAATGCTTTATTTCGGCGCGCACTAAAATCATGGCAGGCTTTGGCAATCACGTCTTTTCCCGTGCCGGTTTCACCTTGAATCACCAACGGCGCATCCAATAACGCAAATTTTCTGGCTTTCGCAATAACATCCCGCATTCGCGGATTTCGGCTGACGATGTGTGAAAAAGGATCGGTGGTTga                            >  1:819305/1‑251 (MQ=255)                                                                                                                                                                                                                ttaCGCCGCCCGAACATTTCCGTTTCCGCGTCTTCGCCGGGAATGCCGGCACAGTTTACGGCGAGAAATGCTTTATTTCGGCGCGCACTAAAATCATGGCAGGCTTTGGCAATCACGTCTTTTCCCGTGCCGGTTTCACCTTGAATCACCAACGGCGCATCCAATAACGCAAATTTTCTGGCTTTCGCAATAACATCCCGCATTCGCGGATTTCGGCTGACGATGTGTGAAAAAGGATCGGTGGTTgaaga                         <  1:813547/251‑1 (MQ=255)                                                                                                                                                                                                                ttaCGCCGCCCGAACATTTCCGTTTCCGCGCCTTCGCCGGGAATGCCGGCACAGTTTACGGCGAGAAATGCTTTATTTCGGCGCGCACTAAAATCATGGCAGGCTTTGGCAATCACGTCTTTTCCCGTGCCGGTTTCACCTTGAATCACCAACGGCGCATCCAATAACGCAAATTTTCTGGCTTTCGCAATAACATCCCGCATTCGCGGATTTCGGCTGACGATGTGTGAAAAAGGATCGGTGGTTgaaga                         <  1:124702/251‑1 (MQ=255)                                                                                                                                                                                                                            aaCATTTCCGTTTCCGCGTCTTCGCCGGGAATGCCGGCACAGTTTACGGCGAGAAATGCTTTATTTCGGCGCGCACTAAAATCATGGCAGGCTTTGGCAATCACGTCTTTTCCCGTGCCGGTTTCACCTTGAATCACCAACGGCGCATCCAATAACGCAAATTTTCTGGCTTTCGCAATAACATCCCGCATTCGCGGATTTCGGCTGACGATGTGTGAAAAAGGATCGGTGGTTGAAGAAGTTGCCATAAg             <  1:896606/251‑1 (MQ=255)                                                                                                                                                                                                                             aCATTTCCGTTTCCGCGTCTTCGCCGGGAATGCCGGCACAGTTTACGGCGAGAAATGCTTTATTTCGGCGCGCACTAAAATCATGGCAGGCTTTGGCAATCACGTCTTTTCCCGTGCCGGTTTCACCTTGAATCACCAACGGCGCATCCAATAACGCAAATTTTCTGGCTTTCGCAATAACATCCCGCATTCGCGGATTTCGGCTGACGATGTGTGAAAAAGGATCGGTGGTTGAAGAAGTTGCCATAAGt            <  1:615687/251‑1 (MQ=255)                                                                                                                                                                                                                             aCATTTCCGTTTCCGCGTCTTCGCCGGGAATGCCGGCACAGTTTACGGCGAGAAATGCTTTATTTCGGCGCGCACTAAAATCATGGCAGGCTTTGGCAATCACGTCTTTTCCCGTGCCGGTTTCACCTTGAATCACCAACGGCGCATCCAATAACGCAAATTTTCTGGCTTTCGCAATAACATCCCGCATTCGCGGATTTCGGCTGACGATGTGTGAAAAAGGATCGGTGGTTGAAGAAGTTGCCATAAGt            <  1:583935/251‑1 (MQ=255)                                                                                                                                                                                                                             aCATTTCCGTTTACGCGTCTTCGCCGGGAATGCCGGCACAGTTTACGGCGAGAAATGCTTTATTTCGGCGCGCACTAAAATCATGGCAGGCTTTGGCAATCACGTCTTTTCCCGTGCCGGTTTCACCTTGAATCACCAACGGCGCATCCAATAACGCAAATTTTCTGGCTTTCGCAATAACATCCCGCATTCGCGGATTTCGGCTGACGATGTGTGAAAAAGGATCGGTGGTTGAAGAAGTTGCCATAAGt            <  1:269569/251‑1 (MQ=255)                                                                                                                                                                                                                              cATTTCCGTTTCCGCGTCTTCGCCGGGAATGCCGGCACAGTTTACGGCGAGAAATGCTTTATTTCGGCGCGCACTAAAATCATGGCAGGCTTTGGCAATCACGTCTTTTCCCGTGCCGGTTTCACCTTGAATCACCAACGGCGCATCCAATAACGCAAATTTTCTGGCTTTCGCAATAACATCCCGCATTCGCGGATTTCGGCTGACGATGTGTGAAAAAGGATCGGTGGTTGAAGAAGTTGCCATAAg             <  1:342066/249‑1 (MQ=255)                                                                                                                                                                                                                                       ttccgCGTCTTCGCCGGGAATGCCGGCACAGTTTACGGCGAGAAATGCTTTATTTCGGCGCGCACTAAAATCATGGCAGGCTTTGGCAATCACGTCTTTTCCCGTGCCGGTTTCACCTTGAATCACCAACGGCGCATCCAATAACGCAAATTTTCTGGCTTTCGCAATAACATCCCGCATTCGCGGATTTCGGCTGACGATGTGTGAAAAAGGATCGGTGGTTGAAGAAGTTGCCATAAGTGCCTCaataa  <  1:392587/251‑1 (MQ=255)                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                  ACATAATGCTGTAACGGCTGCTGCGCCGTGCAAATCACACGTACATTGGCATAATGCTCCTCTTCCTCTCCGACACGGCGGAAAGTACCGTCGCTTAAAAAACGCAGAAGCTTCGCCTGCAAAGTCAGAGGCAACTCCTCAACACCATCTAACAACACCGTGCCGCCATCGGCATATTCAAAAAAACCGATAAATTCACCATCTTTATTACGCCGCCCGAACATTTCCGTTTCCGCGTCCTCGCCGGGAATGCCGGCACAGTTTACGGCGAGAAATGCTTTATTTCGGCGCGCACTAAAATCATGGCAGGCTTTGGCAATCACGTCTTTTCCCGTGCCGGTTTCACCTTGAATCACCAACGGCGCATCCAATAACGCAAATTTTCTGGCTTTCGCAATAACATCCCGCATTCGCGGATTTCGGCTGACGATGTGTGAAAAAGGATCGGTGGTTGAAGAAGTTGCCATAAGTGCCTCAATAA  >  1/1655184‑1655664

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑