Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,654,301	A→T	intergenic (+12462/+10074)	unknown → / ← unknown	tRNA‑Gly‑GCC/LSU rRNA ## 23S rRNA, large subunit ribosomal RNA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,654,301	0	A	T	100.0%	137.6 / NA	52	intergenic (+12462/+10074)	unknown/unknown	tRNA‑Gly‑GCC/LSU rRNA ## 23S rRNA, large subunit ribosomal RNA
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (33/19);  total (33/19)

TTCCCGTTTCAATTTATTTGGTAAACGGTATCAAATTACAGGGGCAAATCGAGTCATTCGATCAATTCGTGATTTTACTGAAAAACACAGTAAATCAAATGGTTTATAAACACGCCATCTCGACTGTTGTACCGGCGCGTTCCGTCGCCCATCACAATGCTAACCAACAGCAGCAACATCAGGGACAACAGCAAGAAACTACTCCTGCCGAAAACAATGTTGAAGCTCAGGCAGAATAACGAAGCTGAAAATATTGAACCCTTTACTCAATAATTTAAGCCAAAGTGCGGTGGATTCTGCGGAAATTTCCACCGCACTTTTTGCGTCTGATAAGCCTGATAATCCCTCATCACATGAAAAATCATGGATTGAATCATGCCACTATCGTGCATTGCCTTTTTGATTCACAAAAAAATACGGATGATTTAAATGAATTTCAACTGCTCACGAAATCCGCTCAAGACAATATTTT  >  1/1654073‑1654544                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                    ttCCCGTTTCAATTTATTTGGTAAACGGTATCAAATTACAGGGGCAAATCGAGTCATTCGATCAATTCGTGATTTTACTGAAAAACACAGTAAATCAAATGGTTTATAAACACGCCATCTCGACTGTTGTACCGGCGCGTTCCGTCGCCCATCACAATGCTAACCAACAGCAGCAACATCAGGGACAACAGCAAGAAACTACTCCTGCCGAAAACAATGTTGAAGCTCTGGc                                                                                                                                                                                                                                                  <  1:648409/232‑1 (MQ=255)                    ggTAAACGGTATCAAATTACAGGGGCAAATCGAGTCATTCGATCAATTCGTGATTTTACTGAAAAACACAGTAAATCAAATGGTTTATAAACACGCCATCTCGACTGTTGTACCGGCGCGTTCCGTCGCCCATCACAATGCTAACCAACAGCAGCAACATCAGGGACAACAGCAAGAAACTACTCCTGCCGAAAACAATGTTGAAGCTCTGGCAGAATAACGAAGCTGAAAATATTGAACCCTTTACTCaa                                                                                                                                                                                                            >  1:619742/1‑251 (MQ=255)                               tCAAATTACAGGGGCAAATCGAGTCATTCGATCAATTCGTGATTTTACTGAAAAACACAGTAAATCAAATGGTTTATAAACACGCCATCTCGACTGTTGTACCGGCGCGTTCCGTCGCCCATCACAATGCTAACCAACAGCAGCAACATCAGGGACAACAGCAAGAAACTACTCCTGCCGAAAACAATGTTGAAGCTCTGGCAGAATAACGAAGCTGAAAATATTGAACCCTTTACTCAATAATTTAAGcc                                                                                                                                                                                                 <  1:693779/251‑1 (MQ=255)                                 aaaTTACAGGGGCAAATCGAGTCATTCGATCAATTCGTGATTTTACTGAAAAACACAGTAAATCAAATGGTTTATAAACACGCCATCTCGACTGTTGTACCGGCGCGTTCCGTCGCCCATCACAATGCTAACCAACAGCAGCAACATCAGGGACAACAGCAAGAAACTACTCCTGCCGAAAACAATGTTGAAGCTCTGGCAGAATAACGAAGCTGAAAATATTGAACCCTTTACTCAATAATTTAAGCCaa                                                                                                                                                                                               >  1:486372/1‑251 (MQ=255)                                 aaaTTACAGGGGCAAATCGAGTCATTCGATCAATTCGTGATTTTACTGAAAAACACAGTAAATCAAATGGTTTATAAACACGCCATCTCGACTGTTGTACCGGCGCGTTCCGTCGCCCATCACAATGCTAACCAACAGCAGCAACATCAGGGACAACAGCAAGAAACTACTCCTGCCGAAAACAATGTTGAAGCTCTGGCAGAATAACGAAGCTGAAAATATTGAACCCTTTACTCAATAATTTAAGCCaa                                                                                                                                                                                               >  1:581972/1‑251 (MQ=255)                                  aaTTACAGGGGCAAATCGAGTCATTCGATCAATTCGTGATTTTACTGAAAAACACAGTAAATCAAATGGTTTATAAACACGCCATCTCGACTGTTGTACCGGCGCGTTCCGTCGCCCATCACAATGCTAACCAACAGCAGCAACATCAGGGACAACAGCAAGAAACTACTCCTGCCGAAAACAATGTTGAAGCTCTGGCAGAATAACGAAGCTGAAAATATTGAACCCTTTACTCAATAATTTAAGCCaaa                                                                                                                                                                                              >  1:377364/1‑251 (MQ=255)                                         ggggCAAATCGAGTCATTCGATCAATTCGTGATTTTACTGAAAAACACAGTAAATCAAATGGTTTATAAACACGCCATCTCGACTGTTGTACCGGCGCGTTCCGTCGCCCATCACAATGCTAACCAACAGCAGCAACATCAGGGACAACAGCAAGAAACTACTCCTGCCGAAAACAATGTTGAAGCTCTGGCAGAATAACGAAGCTGAAAATATTGAACCCTTTACTCAATAATTTAAGCCAAAGTGCGGt                                                                                                                                                                                       >  1:18169/1‑251 (MQ=255)                                         ggggCAAATCGAGTCATTCGATCAATTCGTGATTTTACTGAAAAACACAGTAAATCAAATGGTTTATAAACACGCCATCTCGACTGTTGTACCGGCGCGTTCCGTCGCCCATCACAATGCTAACCAACAGCAGCAACATCAGGGACAACAGCAAGAAACTACTCCTGCCGAAAACAATGTTGAAGCTCTGGCAGAATAACGAAGCTGAAAATATTGAACCCTTTACTCAATAATTTAAGCCAAAGTGCGGt                                                                                                                                                                                       >  1:343690/1‑251 (MQ=255)                                           ggCAAATCGAGTCATTCGATCAATTCGTGATTTTACTGAAAAACACAGTAAATCAAATGGTTTATAAACACGCCATCTCGACTGTTGTACCGGCGCGTTCCGTCGCCCATCACAATGCTAACCAACAGCAGCAACATCAGGGACAACAGCAAGAAACTACTCCTGCCGAAAACAATGTTGAAGCTCTGGCAGAATAACGAAGCTGAAAATATTGAACCCTTTACTCAATAATTTAAGCCAAAGTGCGGTg                                                                                                                                                                                      <  1:211557/250‑1 (MQ=255)                                               aaTCGAGTCATTCGATCAATTCGTGATTTTACTGAAAAACACAGTAAATCAAATGGTTTATAAACACGCCATCTCGACTGTTGTACCGGCGCGTTCCGTCGCCCATCACAATGCTAACCAACAGCAGCAACATCAGGGACAACAGCAAGAAACTACTCCTGCCGAAAACAATGTTGAAGCTCTGGCAGAATAACGAAGCTGAAAATATTGAACCCTTTACTc                                                                                                                                                                                                              >  1:318507/1‑222 (MQ=255)                                                       cATTCGATCAATTCGTGATTTTACTGAAAAACACAGTAAATCAAATGGTTTATAAACACGCCATCTCGACTGTTGTACCGGCGCGTTCCGTCGCCCATCACAATGCTAACCAACAGCAGCAACATCAGGGACAACAGCAAGAAACTACTCCTGCCGAAAACAATGTTGAAGCTCTGGCAGAATAACGAAGCTGAAAATATTGAACCCTTTACTCAATAATTTAAGCCAAAGTGCGGTGGATTCTGCGGaaa                                                                                                                                                                         >  1:493368/1‑251 (MQ=255)                                                         ttCGATCAATTCGTGATTTTACTGAAAAACACAGTAAATCAAATGGTTTATAAACACGCCATCTCGACTGTTGTACCGGCGCGTTCCGTCGCCCATCACAATGCTAACCAACAGCAGCAACATCAGGGACAACAGCAAGAAACTACTCCTGCCGAAAACAATGTTGAAGCTCTGGCAGAATAACGAAGCTGAAAATATTGAACCCTTTACTCAATAATTTAAGCCAAAGTGCGGTGGATTCTGCGGAAAtt                                                                                                                                                                       <  1:655830/251‑1 (MQ=255)                                                             aTCAATTCGTGATTTTACTGAAAAACACAGTAAATCAAATGGTTTATAAACACGCCATCTCGACTGTTGTACCGGCGCGTTCCGTCGCCCATCACAATGCTAACCAACAGCAGCAACATCAGGGACAACAGCAAGAAACTACTCCTGCCGAAAACAATGTTGAAGCTCTGGCAGAATAACGAAGCTGAAAATATTGAACCCTTTACTCAATAATTTAAGCCAAAGTGCGGTGGATTCTGCGGAAATTTCCa                                                                                                                                                                   >  1:795691/1‑251 (MQ=255)                                                              tCAATTCGTGATTTTACTGAAAAACACAGTAAATCAAATGGTTTATAAACACGCCATCTCGACTGTTGTACCGGCGCGTTCCGTCGCCCATCACAATGCTAACCAACAGCAGCAACATCAGGGACAACAGCAAGAAACTACTCCTGCCGAAAGCAATGTTGAAGCTCTGGCAGAATAACGAAGCTGAAAATATTGAACCCTTTACTCAATAATTTAAGCCAAAGTGCGGTGGATTCTGCGGAAATTTCCAc                                                                                                                                                                  <  1:1930/251‑1 (MQ=255)                                                                             aCTGAAAAACACAGTAAATCAAATGGTTTATAAACACGCCATCTCGACTGTTGTACCGGCGCGTTCCGTCGCCCATCACAATGCTAACCAACAGCAGCAACATCAGGGACAACAGCAAGAAACTACTCCTGCCGAAAACAATGTTGAAGCTCTGGCAGAATAACGAAGCTGAAAATATTGAACCCTTTACTCAATAATTTAAGCCAAAGTGCGGTGGATTCTGCGGAAATTTCCACCGCACTTTTTGCGTc                                                                                                                                                   <  1:139217/251‑1 (MQ=255)                                                                                gAAAAACACAGTAAATCAAATGGTTTATAAACACGCCATCTCGACTGTTGTACCGGCGCGTTCCGTCGCCCATCACAATGCTAACCAACAGCAGCAACATCAGGGACAACAGCAAGAAACTACTCCTGCCGAAAACAATGTTGAAGCTCTGGCAGAATAACGAAGCTGAAAATATTGAACCCTTTACTCAATAATTTAAGCCAAAGTGCGGTgg                                                                                                                                                                                     >  1:804321/1‑214 (MQ=255)                                                                                gAAAAACACAGTAAATCAAATGGTTTATAAACACGCCATCTCGACTGTTGTACCGGCGCGTTCCGTCGCCCATCACAATGCTAACCAACAGCAGCAACATCAGGGACAACAGCAAGAAACTACTCCTGCCGAAAACAATGTTGAAGCTCTGGCAGAATAACGAAGCTGAAAATATTGAACCCTTTACTCAATAATTTAAGCCAAAGTGCGGTGGATTCTGCGGAAATTTCCACCGCACTTTTTGCGTCTGa                                                                                                                                                >  1:563017/1‑251 (MQ=255)                                                                                        caGTAAATCAAATGGTTTATAAACACGCCATCTCGACTGTTGTACCGGCGCGTTCCGTCGCCCATCACAATGCTAACCAACAGCAGCAACATCAGGGACAACAGCAAGAAACTACTCCTGCCGAAAACAATGTTGAAGCTCTGGCAGAATAACGAAGCTGAAAATATTGAACCCTTTACt                                                                                                                                                                                                               >  1:322424/1‑180 (MQ=255)                                                                                            aaaTCAAATGGTTTATAAACACGCCATCTCGACTGTTGTACCGGCGCGTTCCGTCGCCCATCACAATGCTAACCAACAGCAGCAACATCAGGGACAACAGCAAGAAACTACTCCTGCCGAAAACAATGTTGAAGCTCTGGCAGAATAACGAAGCTGAAAATATTGAACCCTTTACTCAATAATTTAAGCCAAAGTGCGGTGGATTCTGCGGAAATTTCCACCGCACTTTTTGCGTCTGATAAGCCTGATaa                                                                                                                                    <  1:330467/251‑1 (MQ=255)                                                                                              aTCAAATGGTTTATAAACACGCCATCTCGACTGTTGTACCGGCGCGTTCCGTCGCCCATCACAATGCTAACCAACAGCAGCAACATCAGGGACAACAGCAAGAAACTACTCCTGCCGAAAACAATGTTGAAGCTCTGGCAGAATAACGAAGCTGAAAATATTGAACCCTTTACTCAATAATTTAAGCCAAAGTGCGGTGGATTCTGCGGAAATTTCCACCGCACTTTTTGCGTCTGATAAGCCTGATAAt                                                                                                                                   >  1:311997/1‑250 (MQ=255)                                                                                                    tGGTTTATAAACACGCCATCTCGACTGTTGTACCGGCGCGTTCCGTCGCCCATCACAATGCTAACCAACAGCAGCAACATCAGGGACAACAGCAAGAAACTACTCCTGCCGAAAACAATGTTGAAGCTCTGGCAGAATAACGAAGCTGAAAATATTGAACCCTTTACTCAATAATTTAAGCCAAAGTGCGGTGGATTCTGCGGAAATTTCCACCGCACTTTTTGCGTCTGATAAGCCTGATAATCCCtcat                                                                                                                            >  1:582595/1‑251 (MQ=255)                                                                                                     ggTTTATAAACACGCCATCTCGACTGTTGTACCGGCGCGTTCCGTCGCCCATCACAATGCTAACCAACAGCAGCAACATCAGGGACAACAGCAAGAAACTACTCCTGCCGAAAACAATGTTGAAGCTCTGGCAGAATAACGa                                                                                                                                                                                                                                        >  1:640132/1‑142 (MQ=255)                                                                                                      gTTTATAAACACGCCATCTCGACTGTTGTACCGGCGCGTTCCGTCGCCCATCACAATGCTAACCAACAGCAGCAACATCAGGGACAACAGCAAGAAACTACTCCTGCCGAAAACAATGTTGAAGCTCTGGCAGAATAACGAAGCTGAAAATATTGAACCCTTTACTCAATAAttt                                                                                                                                                                                                      >  1:314619/1‑175 (MQ=255)                                                                                                      gTTTATAAACACGCCATCTCGACTGTTGTACCGGCGCGTTCCGTCGCCCATCACAATGCTAACCAACAGCAGCAACATCAGGGACAACAGCAAGAAACTACTCCTGCCGAAAACAATGTTGAAGCTCTGGCAGAATAACGAAGCTGAAAATATTGAACCCTTTACTCAATAATTTAAGCCAAAGTGCGGTgg                                                                                                                                                                                     <  1:590590/192‑1 (MQ=255)                                                                                                      gTTTATAAACACGCCATCTCGACTGTTGTACCGGCGCGTTCCGTCGCCCATCACAATGCTAACCAACAGCAGCAACATCAGGGACAACAGCAAGAAACTACTCCTGCCGAAAACAATGTTGAAGCTCTGGCAGAATAACGAAGCTGAAAATATTGAACCCTTTACTCAATAATTTAAGCCAAAGTGCGGTGGATTCTGCGGAAATTTCCACCGCACTTTTTGCGTCTGATAAGCCTGATAATccc                                                                                                                                >  1:56935/1‑245 (MQ=255)                                                                                                                     aTCTCGACTGTTGTACCGGCGCGTTCCGTCGCCCATCACAATGCTAACCAACAGCAGCAACATCAGGGACAACAGCAAGAAACTACTCCTGCCGAAAACAATGTTGAAGCTCTGGCAGAATAACGAAGCTGAAAATATTGAACCCTTTACTCAATAATTTAAGCCAAAGTGCGGTGGaaatttccgcagaatcc                                                                                                                                                                    >  1:257746/1‑178 (MQ=255)                                                                                                                       ctcGACTGTTGTACCGGCGCGTTCCGTCGCCCATCACAATGCTAACCAACAGCAGCAACATCAGGGACAACAGCAAGAAACTACTCCTGCCGAAAACAATGTTGAAGCTCTGGCAGAATAACGAAGCTGAAAATATTGAACCCTTTACTCAATAATTTAAGCCAAAGTGCGGTGGATTCTGCGGAAATTTCCACCGCACTTTTTGCGTCTGATAAGCCTGATAATCCCTCATCACATGAAAAATCATGGAt                                                                                                         >  1:604719/1‑251 (MQ=255)                                                                                                                            cTGTTGTACCGGCGCGTTCCGTCGCCCATCACAATGCTAACCAACAGCAGCAACATCAGGGACAACAGCAAGAAACTACTCCTGCCGAAAACAATGTTGAAGCTCTGGCAGAATAACGAAGCTGAAAATATTGAACCCTTTACTCAATAATTTAAGCCAAAGTGCGGTGGATTCTGCGGAAATTTCCACCGCACTTTTTGCGTCTGATAAGCCTGATAATCCCTCATCACATGAAAAAt                                                                                                                >  1:665595/1‑239 (MQ=255)                                                                                                                              gttgtACCGGCGCGTTCCGTCGCCCATCACAATGCTAACCAACAGCAGCAACATCAGGGACAACAGCAAGAAACTACTCCTGCCGAAAACAATGTTGAAGCTCTGGCAGAATAACGAAGCTGAAAATATTGAACCCTTTACTCAATAATTTAAGCCAAAGTGCGGTGGATTCTGCGGAAATTTCCACCGCACTTTTTGCGTCTGATAAGCCTGATAATCCCTCATCACATGAAAAATCATGGATTGAATCa                                                                                                  >  1:140408/1‑251 (MQ=255)                                                                                                                                      ggCGCGTTCCGTCGCCCATCACAATGCTAACCAACAGCAGCAACATCAGGGACAACAGCAAGAAACTACTCCTGCCGAAAACAATGTTGAAGCTCTGGCAGAATAACGAAGCTGAAAATATTGAACCCTTTACTCAATAATTTAAGCCAAAGTGCGGTGGATTCTGCGGAAATTTCCACCGCACTTTTTGCGTCTGATAAGCCTGATAATCCCTCATCACATGAAAAATCATGGATTGAATCATGc                                                                                               <  1:707623/246‑1 (MQ=255)                                                                                                                                        cgcgTTCCGTCGCCCATCACAATGCTAACCAACAGCAGCAACATCAGGGACAACAGCAAGAAACTACTCCTGCCGAAAACAATGTTGAAGCTCTGGCAGAATAACGAAGCTGAAAATATTGAACCCTTTACTCAATAATTTAAGCCAAAGTGCGGTGGATTCTGCGGAAATTTCCACCGCACTTTTTGCGTCTGATAAGCCTGATAATCCCTCATCACATGAAAAATCATGGATTGAATCATGCCACTAt                                                                                         <  1:103718/250‑1 (MQ=255)                                                                                                                                        cgcgTTCCGTCGCCCATCACAATGCTAACCAACAGCAGCAACATCAGGGACAACAGCAAGAAACTACTCCTGCCGAAAACAATGTTGAAGCTCTGGCAGAATAACGAAGCTGAAAATATTGAACCCTTTACTCAATAATTTAAGCCAAAGTGCGGTGGATTCTGCGGAAATTTCCACCGCACTTTTTGCGTCTGATAAGCCTGATAATCCCTCATCACATGAAAAATCATGGATTGAATCATGCCACTAt                                                                                         <  1:761415/250‑1 (MQ=255)                                                                                                                                                        ccctgatgttgctgctgttggttaaCATCAGGGACAACAGCAAGAAACTACTCCTGCCGAAAACAATGTTGAAGCTCTGGCAGAATAACGAAGCTGAAAATATTGAACCCTTTACTCAATAATTTAAGCCAAAGTGCGGTGGATTCTGCGGAAATTTCCACCGCACTTTTTGCGTCTGATAAGCCTGATAATCCCTCATCACATGAAAAATCATGGATTGAATCATGCCACTATCGTGCATTGCCTTTTTg                                                                        >  1:74760/24‑251 (MQ=255)                                                                                                                                                           caATGCTAACCAACAGCAGCAACATCAGGGACAACAGCAAGAAACTACTCCTGCCGAAAACAATGTTGAAGCTCTGGCAGAATAACGAAGCTGAAAATATTGAACCCTTTACTCAATAATTTAAGCCAAAGTGCGGTGGATTCTGCGGAAATTTCCACCGCACTTTTTGCGTCTGATAAGCCTGATAATCCCTCATCACATGAAAAATCATGGATTGAATCATGCCACTATCGTGCATTGCCTTTTTGAtt                                                                     >  1:217507/1‑251 (MQ=255)                                                                                                                                                             aTGCTAACCAACAGCAGCAACATCAGGGACAACAGCAAGAAACTACTCCTGCCGAAAACAATGTTGAAGCTCTGGCAGAATAACGAAGCTGAAAATATTGAACCCTTTACTCAATAATTTAAGCCAAAGTGCGGTGGATTCTGCGGAAATTTCCACCGCACTTTTTGCGTCTGATAAGCCTGATAATCCCTCATCACATGAAAAATCATGGATTGAATCATGCCACTATCGTGCATTGCCTTTTTGATTca                                                                   >  1:310064/1‑251 (MQ=255)                                                                                                                                                                  aaCCAACAGCAGCAACATCAGGGACAACAGCAAGAAACTACTCCTGCCGAAAACAATGTTGAAGCTCTGGCAGAATAACGAAGCTGAAAATATTGAACCCTTTACTCAATAATTTAAGCCAAAGTGCGGTGGATTCTGCGGAAATTTCCACCGCACTTTTTGCGTCTGATAAGCCTGATAAt                                                                                                                                   >  1:800535/1‑182 (MQ=255)                                                                                                                                                                  aaCCAACAGCAGCAACATCAGGGACAACAGCAAGAAACTACTCCTGCCGAAAACAATGTTGAAGCTCTGGCAGAATAACGAAGCTGAAAATATTGAACCCTTTACTCAATAATTTAAGCCAAAGTGCGGTGGATTCTGCGGAAATTTCCACCGCACTTTTTGCGTCTGATAAGCCTGATAATCCCTCATCACATGAAAAATCATGGATTGAATCATGCCACTAt                                                                                         >  1:74192/1‑224 (MQ=255)                                                                                                                                                                        cagcagCAACATCAGGGACAACAGCAAGAAACTACTCCTGCCGAAAACAATGTTGAAGCTCTGGCAGAATAACGAAGCTGAAAATATTGAACCCTTTACTCAATAATTTAAGCCAAAGTGCGGTGGATTCTGCGGAAATTTCCACCGCACTTTTTGCGTCTGATAAGCCTGATAATCCCTCATCACATGAAAAATCATGGATTGAATCATGCCACTATCGTGCAt                                                                                  <  1:77250/225‑1 (MQ=255)                                                                                                                                                                           cagcaACATCAGGGACAACAGCAAGAAACTACTCCTGCCGAAAACAATGTTGAAGCTCTGGCAGAATAACGAAGCTGAAAATATTGAACCCTTTACTCAATAATTTAAGCCAAAGTGCGGTGGATTCTGCGGAAATTTCCACCGCACTTTTTGCGTCTGATAAGCCTGATAATCCCTCATCACATGAAAAATCATGGATTGAATCATGCCACTATCGTGCATTGCCTTTTTGATTCACAAAAAAATAc                                                        <  1:601855/248‑1 (MQ=255)                                                                                                                                                                           cagcaACATCAGGGACAACAGCAAGAAACTACTCCTGCCGAAAACAATGTTGAAGCTCTGGCAGAATAACGAAGCTGAAAATATTGAACCCTTTACTCAATAATTTAAGCCAAAGTGCGGTGGATTCTGCGGAAATTTCCACCGCACTTTTTGCGTCTGATAAGCCTGATAATCCCTCATCACATGAAAAATCATGGATTGAATCATGCCACTATCGTGCATTGCCTTTTTGATTCACAAAAAAATAc                                                        <  1:781674/248‑1 (MQ=255)                                                                                                                                                                              caACATCAGGGACAACAGCAAGAAACTACTCCTGCCGAAAACAATGTTGAAGCTCTGGCAGAATAACGAAGCTGAAAATATTGAACCCTTTACTCAATAATTTAAGCCAAAGAGCGGTGGATTCTGCGGAAATTTCCACCGCACTTTTTGCGTCTGATAAGCCTGATAATCCCTCATCACATGAAAAATCATGGATTGAATCATGCCACTATCGTGCATTGCCTTTTTGATTCACAAAAAAATACGgatga                                                  >  1:52452/1‑251 (MQ=255)                                                                                                                                                                                 cATCAGGGACAACAGCAAGAAACTACTCCTGCCGAAAACAATGTTGAAGCTCTGGCAGAATAACGAAGCTGAAAATATTGAACCCTTTACTCAATAATTTAAGCCAAAGTGCGGTGGATTCTGCGGAAATTTCCACCGCACTTTTTGCGTCTGATAAGCCTGATAATCCCTCATCACATGAAAAATCATGGATTGAATCATGCCACTATCg                                                                                       <  1:8712/211‑1 (MQ=255)                                                                                                                                                                                 cATCAGGGACAACAGCAAGAAACTACTCCTGCCGAAAACAATGTTGAAGCTCTGGCAGAATAACGAAGCTGAAAATATTGAACCCTTTACTCAATAATTTAAGCCAAAGTGCGGTGGATTCTGCGGAAATTTCCACCGCACTTTTTGCGTCTGATAAGCCTGATAATCCCTCATCACATGAAAAATCATGGATTGAATCATGCCACTATCGTGCATTGCCTTTTTGATTCACAAAAAAATACGGATGAttt                                               <  1:601041/251‑1 (MQ=255)                                                                                                                                                                                  aTCAGGGACAACAGCAAGAAACTACTCCTGCCGAAAACAATGTTGAAGCTCTGGCAGAATAACGAAGCTGAAAATATTGAACCCTTTACTCAATAATTTAAGCCAAAGTGCGGTGGATTCTGCGGAAATTTCCACCGCACTTTTTGCGTCTGATAAGCCTGATAATCCCTCATCACATGAAAAATCATGGATTGAATCATGCCACTATCGTGCATTGCCTTTTTGATTCACAAAAAAAt                                                          >  1:549576/1‑239 (MQ=255)                                                                                                                                                                                   tCAGGGACAACAGCAAGAAACTACTCCTGCCGAAAACAATGTTGAAGCTCTGGCAGAATAACGAAGCTGAAAATATTGAACCCTTTACTCAATAATTTAAGCCAAAGTGCGGTGGATTCTGCGGAAATTTCCACCGCACTTTTTGCGTCTGATAAGCCTGATAATCCCTCATCACATGAAAAATCATGGATTGAATCATGCCACTATCGTGCATTGCCTTTTTGATTCACAAAAAAATACGGATGATTTaa                                             >  1:128792/1‑251 (MQ=255)                                                                                                                                                                                                 aaGAAACTACTCCTGCCGAAAACAATGTTGAAGCTCTGGCAGAATAACGAAGCTGAAAATATTGAACCCTTTACTCAATAATTTAAGCCAAAGTGCGGTGGATTCTGCGGAAATTTCCACCGCACTTTTTGCGTCTGATAAGCCTGATAATCCCTCATCACATGAAAAATCATGGATTGAATCATGCCACTATCGTGCATTGCCTTTTTGATTCACAAAAAAATACGGATGATTTAAATGAATTTCAACTg                               <  1:375979/251‑1 (MQ=255)                                                                                                                                                                                                    aaaCTACTCCTGCCGAAAACAATGTTGAAGCTCTGGCAGAATAACGAAGCTGAAAATATTGAACCCTTTACTCAATAATTTAAGCCAAAGTGCGGTGGATTCTGCGGAAATTTCCACCGCACTTTTTGCGTCTGATAAGCCTGATAATCCCTCATCACATGAAAAATCATGGATTGAATCATGCCACTATCGTGCAt                                                                                  >  1:852710/1‑197 (MQ=255)                                                                                                                                                                                                    aaaCTACTCCTGCCGAAAACAATGTTGAAGCTCTGGCAGAATAACGAAGCTGAAAATATTGAACCCTTTACTCAATAATTTAAGCCAAAGTGCGGTGGATTCTGCGGAAATTTCCACCGCACTTTTTGCGTCTGATAAGCCTGATAATCCCTCATCACATGAAAAATCATGGATTGAATCATGCCACTATCGTGCATTGCCTTTTTGATTCACAAAAAAATACGGATGATTTAAATGAATTTCAACTGCt                             >  1:401504/1‑250 (MQ=255)                                                                                                                                                                                                         actCCTGCCGAAAACAATGTTGAAGCTCTGGCAGAATAACGAAGCTGAAAATATTGAACCCTTTACTCAATAATTTAAGCCAAAGTGCGGTGGATTCTGCGGAAATTTCCACCGCACTTTTTGCGTCTGATAAGCCTGATAATCCCTCATCACATGAAAAATCATGGATTGAAt                                                                                                    >  1:372553/1‑174 (MQ=255)                                                                                                                                                                                                                      aCAATGTTGAAGCTCTGGCAGAATAACGAAGCTGAAAATATTGAACCCTTTACTCAATAATTTAAGCCAAAGTGCGGTGGATTCTGCGGAAATTTCCACCGCACTTTTTGCGTCTGATAAGCCTGATAATCCCTCATCACATGAAAAATCATGGATTGAATCATGCCACTATCGTGCATTGCCTTTTTGATTCACAAAAAAATACGGATGATTTAAATGAATTTCAACTGCTCACGAAATCCGCTCAAGAc          <  1:204798/251‑1 (MQ=255)                                                                                                                                                                                                                           gTTGAAGCTCTGGCAGAATAACGAAGCTGAAAATATTGAACCCTTTACTCAATAATTTAAGCCAAAGTGCGGTGGATTCTGCGGAAATTTCCACCGCACTTTTTGCGTCTGATAAGCCTGATAATCCCTCATCACATGAAAAATCATGGATTGAATCATGCCACTATCGTGCATTGCCTTTTTGATTCACAAAAAAATACGGATGATTTAAATGAATTTCAACTGCTCACGAAATCCGCTCAAGACAatat     >  1:319077/1‑251 (MQ=255)                                                                                                                                                                                                                               aaGCTCTGGCAGAATAACGAAGCTGAAAATATTGAACCCTTTACTCAATAATTTAAGCCAAAGTGCGGTGGATTCTGCGGAAATTTCCACCGCACTTTTTGCGTCTGATAAGCCTGATAATCCCTCATCACATGAAAAATCATGGATTGAATCATGCCACTATCGTGCATTGCCTTTTTGATTCACAAAAAAATACGGATGATTTAAATGAATTTCAACTGCTCACGAAATCCGCTCAAGACAATAtttt  <  1:475803/250‑1 (MQ=255)                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                    TTCCCGTTTCAATTTATTTGGTAAACGGTATCAAATTACAGGGGCAAATCGAGTCATTCGATCAATTCGTGATTTTACTGAAAAACACAGTAAATCAAATGGTTTATAAACACGCCATCTCGACTGTTGTACCGGCGCGTTCCGTCGCCCATCACAATGCTAACCAACAGCAGCAACATCAGGGACAACAGCAAGAAACTACTCCTGCCGAAAACAATGTTGAAGCTCAGGCAGAATAACGAAGCTGAAAATATTGAACCCTTTACTCAATAATTTAAGCCAAAGTGCGGTGGATTCTGCGGAAATTTCCACCGCACTTTTTGCGTCTGATAAGCCTGATAATCCCTCATCACATGAAAAATCATGGATTGAATCATGCCACTATCGTGCATTGCCTTTTTGATTCACAAAAAAATACGGATGATTTAAATGAATTTCAACTGCTCACGAAATCCGCTCAAGACAATATTTT  >  1/1654073‑1654544

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑