Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,652,438	C→T	intergenic (+10599/+11937)	unknown → / ← unknown	tRNA‑Gly‑GCC/LSU rRNA ## 23S rRNA, large subunit ribosomal RNA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,652,438	0	C	T	100.0%	173.9 / NA	65	intergenic (+10599/+11937)	unknown/unknown	tRNA‑Gly‑GCC/LSU rRNA ## 23S rRNA, large subunit ribosomal RNA
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (35/30);  total (35/30)

GACTACCGGTTATTCCGCCACTGCCTACGCCGAAAAAAAGAATGACGGCACGCAAAAACACCGGCAAAGTTCACCGCACTTTTATCGTGAAAATGCCCATCGTGAACAAATTACGCAGAACGTATTGAATGCCCACAAAACCTTGTGGACGGCGTTAAAAAATCAGCCTCAAACGGAAATGACCATAGCGGAAGTCAGCAAACCCAATACCGATTTTTTACACGCGCTTGCGCTTATCAACGACGAGGCATTATTGTTACAGCAAGGACAGCATTTTTATTTGTTGTCATTAGCCCGTTTGCAGCATTTAAAATTGGAGTTGAATTTAACCTTGGCGCCAACATCTCAGCCATTGTTAATCCCGATTATTTTTCGCTTATCTAATGACCAATGGCAGGCATGGTTACAACAAAAAACGTTCTTTGAGCAAATCGGTTTTACTTTTACGGAAGAGGCGACACAACATAAAATAACGCTGCAAAA  >  1/1652201‑1652683                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                                      gACTACCGGTTATTCCGCCACTGCCTACGCCGAAAAAAAGAATGACGGCACGCAAAAACACCGGCAAAGTTCACCGCACTTTTATCGTGAAAATGCCCATCGTGAACAAATTACGCAGAACGTATTGAATGCCCACAAAACCTTGTGGACGGCGTTAAAAAATCAGCCTCAAACGGAAATGACCATAGCGGAAGTCAGCAAACCCAATACCGATTTTTTACACGCGCTTGCGCTTATTAac                                                                                                                                                                                                                                                    >  1:661000/1‑241 (MQ=255)    tACCGGTTATTCCGCCACTGCCTACGCCGAAAAAAAGAATGACGGCACGCAAAAACACCGGCAAAGTTCACCGCACTTTTATCGTGAAAATGCCCATCGTGAACAAATTACGCAGAACGTATTGAATGCCCACAAAACCTTGTGGACGGCGTTAAAAAATCAGCCTCAAACGGAAATGACCATAGCGGAAGTCAGCAAACCCAATACCGATTTTTTACACGCGCTTGCGCTTATTAACGACGAGGCattat                                                                                                                                                                                                                                       <  1:651429/251‑1 (MQ=255)       cGGTTATTCCGCCACTGCCTACGCCGAAAAAAAGAATGACGGCACGCAAAAACACCGGCAAAGTTCACCGCACTTTTATCGTGAAAATGCCCATCGTGAACAAATTACGCAGAACGTATTGAATGCCCACAAAACCTTGTGGACGGCGTTAAAAAATCAGCCTCAAACGGAAATGACCATAGCGGAAGTCAGCAAACCCAATACCGATTTTTTACACGCGCTTGCGCTTATTAACGACGAGGCATTATTg                                                                                                                                                                                                                                     <  1:815034/250‑1 (MQ=255)             ttCCGCCACTGCCTACGCCGAAAAAAAGAATGACGGCACGCAAAAACACCGGCAAAGTTCACCGCACTTTTATCGTGAAAATGCCCATCGTGAACAAATTACGCAGAACGTATTGAATGCCCACAAAACCTTGTGGACGGCGTTAAAAAATCAGCCTCAAACGGAAATGACCATAGCGGAAGTCAGCAAACCCAATACCGATTTTTTACACGCGCTTGCGCTTATTAacga                                                                                                                                                                                                                                                  <  1:206710/231‑1 (MQ=255)                       gCCTACGCCGAAAAAAAGAATGACGGCACGCAAAAACACCGGCAAAGTTCACCGCACTTTTATCGTGAAAATGCCCATCGTGAACAAATTACGCAGAACGTATTGAATGCCCACAAAACCTTGTGGACGGCGTTAAAAAATCAGCCTCAAACGGAAATGACCATAGCGGAAGTCAGCAAACCCAATACCGATTTTTTACACGCGCTTGCGCTTATTAACGACGAGGCATTATTGtt                                                                                                                                                                                                                                   <  1:853309/236‑1 (MQ=255)                                         aaTGACGGCACGCAAAAACACCGGCAAAGTTCACCGCACTTTTATCGTGAAAATGCCCATCGTGAACAAATTACGCAGAACGTATTGAATGCCCACAAAACCTTGTGGACGGCGTTAAAAAATCAGCCTCAAACGGAAATGACCATAGCGGAAGTCAGCAAACCCAATACCGATTTTTTACACGCGCTTGCGCTTATTAACGACGAGGCATTATTGTTACAGCAAGGACAGCATTTTTATTTGTTGTCAtt                                                                                                                                                                                                  <  1:442890/251‑1 (MQ=255)                                                   ctttaatttttgttcatgggtgaCCGCACTTTTATCGTGAAAATGCCCATCGTGAACAAATTACGCAGAACGTATTGAATGCCCACAAAACCTTGTGGACGGCGTTAAAAAATCAGCCTCAAACGGAAATGACCATAGCGGAAGTCAGCAAACCCAATACCGATTTTTTACACGCGCTTGCGCTTATTAACGACGAGGCATTATTGTTACAGCAAGGACAGCATTTTTATTTGTTGTCATTAGCCCGTTTg                                                                                                                                                                                        >  1:621533/23‑251 (MQ=255)                                                   cGCAAAAACACCGGCAAAGTTCACCGCACTTTTATCGTGAAAATGCCCATCGTGAACAAATTACGCAGAACGTATTGAATGCCCACAAAACCTTGTGGACGGCGTTAAAAAATCAGCCTCAAACGGAAATGACCATAGCGGAAGTCAGCAAACCCAATACCGATTTTTTACACGCGCTTGCGCTTATTAACGACGAGGCATTATTGTTACAGCAAGGAc                                                                                                                                                                                                                        <  1:556033/219‑1 (MQ=255)                                                                     gTTCACCGCACTTTTATCGTGAAAATGCCCATCGTGAACAAATTACGCAGAACGTATTGAATGCCCACAAAACCTTGTGGACGGCGTTAAAAAATCAGCCTCAAACGGAAATGACCATAGCGGAAGTCAGCAAACCCAATACCGATTTTTTACACGCGCTTGCGCTTATTAACGACGAGGCATTATTGTTACAGCAAGGACAGCATTTTTATTTGTTGTCATTAGCCCGTTTGCAGCATTTAAAATTGGAg                                                                                                                                                                      >  1:660399/1‑251 (MQ=255)                                                                     gTTCACCGCACTTTTATCGTGAAAATGCCCATCGTGAACAAATTACGCAGAACGTATTGAATGCCCACAAAACCTTGTGGACGGCGTTAAAAAATCAGCCTCAAACGGAAATGACCATAGCGGAAGTCAGCAAACCCAATACCGATTTTTTACACGCGCTTGCGCTTATTAACGACGAGGCATTATTGTTACAGCAAGGACAGCATTTTTATTTGTTGTCATAAGCCCGTTTGCAGAATTTAAAATTGGAg                                                                                                                                                                      >  1:894090/1‑251 (MQ=255)                                                                           cGCACTTTTATCGTGAAAATGCCCATCGTGAACAAATTACGCAGAACGTATTGAATGCCCACAAAACCTTGTGGACGGCGTTAAAAAATCAGCCTCAAACGGAAATGACCATAGCGGAAGTCAGCAAACCCAATACCGATTTTTTACACGCGCTTGCGCTTATTAACGACGAGGCATTATTGTTACAGCAAGGACAGCATTTTTATTTGTTGTCATTAGCCCGTTTGCAGCATTTAAAATTGGAGTTGAAt                                                                                                                                                                <  1:262876/251‑1 (MQ=255)                                                                                ttttATCGTGAAAATGCCCATCGTGAACAAATTACGCAGAACGTATTGAATGCCCACAAAACCTTGTGGACGGCGTTAAAAAATCAGCCTCAAACGGAAATGACCATAGCGGAAGTCAGCAAACCCAATACCGATTTTTTACACGCGCTTGCGCTTATTAACGACGAGGCATTATTGTTACAGCAAGGACAGCA‑TTTTATTTGTTGTCATTAGCCCGTTTGCAGCATTTAAAATTGGAGTTGAATTTAAcc                                                                                                                                                          <  1:606911/251‑1 (MQ=255)                                                                                              tGCCCATCGTGAACAAATTACGCAGAACGTATTGAATGCCCACAAACCCTTGTGGACGGCGTTAAAAAATCAGCCTCAAACGGAAATGACCATAGCGGAAGTCAGCAAACCCAATACCGATTTTTTACACGCGCTTGCGCTTATTAACGACGAGGCATTATTGTTACAGAAAGGACAGCATTTTTATTTGTTGTCATTAGCCCGTTTGCAGCATTTAAAATTGGAGTTGAATTTAACCTTGGCGCCAACAt                                                                                                                                             >  1:696422/1‑251 (MQ=255)                                                                                                   aTCGTGAACAAATTACGCAGAACGTATTGAATGCCCACAAAACCTTGTGGACGGCGTTAAAAAATCAGCCTCAAACGGAAATGACCATAGCGGAAGTCAGCAAACCCAATACCGATTTTTTACACGCGCTTGCGCTTATTAACGACGAGGCATTATTGTTACAGCAAGGACAGCATTTTTATTTGTTGTCATTAGCCCGTTTGCAGCATTTAAAATTGGAGTTGAATTTAAcc                                                                                                                                                          >  1:176913/1‑233 (MQ=255)                                                                                                    tCGTGAACAAATTACGCAGAACGTATTGAATGCCCACAAAACCTTGTGGACGGCGTTAAAAAATCAGCCTCAAACGGAAATGACCATAGCGGAAGTCAGCAAACCCAATACCGATTTTTTACACGCGCTTGCGCTTATTAACGACGAGGCATTATTGTTACAGCAAGGACAGCATTTTTATTTGTTGTCATTAGCCCGTTTGCAGCATTTAAAATTGGAGTTGAATTTAACCTTGGCGCCAACATCTCAg                                                                                                                                        >  1:411029/1‑250 (MQ=255)                                                                                                        gAACAAATTACGCAGAACGTATTGAATGCCCACAAAACCTTGTGGACGGCGTTAAAAAATCAGCCTCAAACGGAAATGACCATAGCGGAAGTCAGCAAACCCAATACCGATTTTTTACACGCGCTTGCGCTTATTAACGACGAGGCATTATTGTTACAGCAAGGACAGCAtttt                                                                                                                                                                                                                <  1:176525/174‑1 (MQ=255)                                                                                                         aaCAAATTACGCAGAACGTATTGAATGCCCACAAAACCTTGTGGACGGCGTTAAAAAATCAGCCTCAAACGGAAATGACCACAGCGGAAGTCAGCAAACCCAATACCGATTTTTTACACGCGCTTGCGCTTATTAACGACGAGGCATTATTGTTACAGCAAGGACAGCATTTTTATTTGTTGTCATTAGCCCGTTTGCAGCATTTAAAATTGGAGTTGAATTTAACCTTGGCGCCAACATCTCAGCCATTg                                                                                                                                  <  1:455897/251‑1 (MQ=255)                                                                                                           cAAATTACGCAGAACGTATTGAATGCCCACAAAACCTTGTGGACGGCGTTAAAAAATCAGCCTCAAACGGAAATGACCATAGCGGAAGTCAGCAAACCCAATACCGATTTTTTACACGCGCTTGCGCTTATTAACGACGAGGCATTATTGTTACAGCAAGGACAGCATTTTTATTTGTTGTCATTAGCCCGTTTGCAGCATTTAAAATTGGAGTTGAATTTAACCTTGGCGCCAACATCTCAGCCATTGtt                                                                                                                                <  1:143967/251‑1 (MQ=255)                                                                                                                tACGCAGAACGTATTGAATGCCCACAAAACCTTGTGGACGGCGTTAAAAAATCAGCCTCAAACGGAAATGACCATAGCGGAAGTCAGCAAACCCAATACCGATTTTTTACACGCGCTTGCGCTTATTAACGACGAGGCATTATTGTTACAGCAAGGACAGCATTTTTATTTGTTGTCATTAGCCCGTTTGCAGCATTTAAAATTGGAGTTGAATTTAACCTTGGCGCCAACATCTCAGCCATTGTTAATcc                                                                                                                           <  1:810333/251‑1 (MQ=255)                                                                                                                 aCGCAGAACGTATTGAATGCCCACAAAACCTTGTGGACGGCGTTAAAAAATCAGCCTCAAACGGAAATGACCATAGCGGAAGTCAGCAAACCCAATACCGATTTTTTACACGCGCTTGCGCTTATTAACGACGAGGCATTATTGTTACAGCAAGGACAGCATTTTTATTTGTTGTCATTAGCCCGTTTGCAGCATTTAAAATTGGAGTTGAATTTAACCTTGGCGCCAACATCTCAGCCATTGTTAATcc                                                                                                                           >  1:414429/1‑250 (MQ=255)                                                                                                                      gAACGTATTGAATGCCCACAAAACCTTGTGGACGGCGTTAAAAAATCAGCCTCAAACGGAAATGACCATAGCGGAAGTCAGCAAACCCAATACCGATTTTTTACACGCGCTTGCGCTTATTAACGACGAGGCATTATTGTTACAGc                                                                                                                                                                                                                              >  1:605858/1‑146 (MQ=255)                                                                                                                       aaCGTATTGAATGCCCACAAAACCTTGTGGACGGCGTTAAAAAATCAGCCTCAAACGGAAATGACCATAGCGGAAGTCAGCAAACCCAATACCGATTTTTTACACGCGCTTGCGCTTATTAACGACGAGGCATTATTGTTACAGCAAGGACAGCATTTTTATTTGTTGTCATTAGCCCGTTTGCAGCATTTAAAATTGGAGTTGAATTTAACCTTGGCGCCAACATCTCAGCCATTGTTAATCCCGatta                                                                                                                     <  1:569437/250‑1 (MQ=255)                                                                                                                       aaCGTATTGAATGCCCACAAAACCTTGTGGACGGCGTTAAAAAATCAGCCTCAAACGGAAATGACCATAGCGGAAGTCAGCAAACCCAATACCGATTTTTTACACGCGCTTGCGCTTATTAACGACGAGGCATTATTGTTACAGCAAGGACAGCATTTTTATTTGTTGTCATTAGCCCGTTTGCAGCATTTAAAATTGGAGTTGAATTTAACCTTGGCGCCAACATCTCAGCCATTGTTAATCCCGatta                                                                                                                     <  1:14662/250‑1 (MQ=255)                                                                                                                       aaCGTATTGAATGCCCACAAAACCTTGTGGACGGCGTTAAAAAATCAGCCTCAAACGGAAATGACCATAGCGGAAGTCAGCAAACCCAATACCGATTTTTTACACGCGCTTGCGCTTATTAACGACGAGGCATTATTGTTACAGCAAGGACAGCATTTTTATTTGTTGTCATTAGCCCGTTTGCAGCATTTAAAATTGGAGTTGAATTTAACCTTGGCGCCAACATCTCAGCCATTGTTAATCCCGatt                                                                                                                      <  1:708195/249‑1 (MQ=255)                                                                                                                        aCGTATTGAATGCCCACAAAACCTTGTGGACGGCGTTAAAAAATCAGCCTCAAACGGAAATGACCATAGCGGAAGTCAGCAAACCCAATACCGATTTTTTACACGCGCTTGCGCTTATTAACGACGAGGCATTATTGTTACAGCAAGGACAGCATTTTTATTTGTTGTCATTAGCCCGTTTgcag                                                                                                                                                                                     <  1:594524/185‑1 (MQ=255)                                                                                                                          gTATTGAATGCCCACAAAACCTTGTGGACGGCGTTAAAAAATCAGCCTCAAACGGAAATGACCATAGCGGAAGTCAGCAAACCCAATACCGATTTTTTACACGCGCTTGCGCTTATTAACGACGAGGCATTATTGTTACAGCAAGGACAGCATTTTTATTTGTTGTCATTAGCCCGTTTGCAGCATTTAAAATTGGAGTTGAATTTAACCTTGGCGCCAACATCTCAGCCATTGtt                                                                                                                                >  1:765230/1‑236 (MQ=255)                                                                                                                          gTATTGAATGCCCACAAAACCTTGTGGACGGCGTTAAAAAATCAGCCTCAAACGGAAATGACCATAGCGGAAGTCAGCAAACCCAATACCGATTTTTTACACGCGCTTGCGCTTATTAACGACGAGGCATTATTGTTACAGCAAGGACAGCATTTTTATTTGTTGTCATTAGCCCGTTTGCAGCATTTAAAATTGGAGTTGAATTTAACCTTGGCGCCAACATCTCAGCCATTGTTAATCCCGATTAtttt                                                                                                                 >  1:891001/1‑251 (MQ=255)                                                                                                                          gTATTGAATGCCCACAAAACCTTGTGGACGGCGTTAAAAAATCAGCCTCAAACGGAAATGACCATAGCGGAAGTCAGCAAACCCAATACCGATTTTTTACACGCGCTTGCGCTTATTAACGACGAGGCATTATTGTTACAGCAAGGACAGCATTTTTATTTGTTGTCATTAGCCCGTTTGCAGCATTTAAAATTGGAGTTGAATTTAACCTTGGCGCCAACATCTCAGCCATTGTTAATCCCGATTAtttt                                                                                                                 >  1:782828/1‑251 (MQ=255)                                                                                                                          gTATTGAATGCCCACAAAACCTTGTGGACGGCGTTAAAAAATCAGCCTCAAACGGAAATGACCATAGCGGAAGTCAGCAAACCCAATACCGATTTTTTACACGCGCTTGCGCTTATTAACGACGAGGCATTATTGTTACAGCAAGGACAGCATTTTTATTTGTTGTCATTAGCCCGTTTGCAGCATTTAAAATTGGAGTTGAATTTAACCTTGGCGCCAACATCTCAGCCATTGTTAATCCCGATTAtttt                                                                                                                 >  1:590535/1‑251 (MQ=255)                                                                                                                            aTTGAATGCCCACAAAACCTTGTGGACGGCGTTAAAAAATCAGCCTCAAACGGAAATGACCATAGCGGAAGTCAGCAAACCCAATACCGATTTTTTACACGCGCTTGCGCTTATTAACGACGAGGCATTATTGTTACAGCAAGGACAGCATTTTTATTTGTTGTCATTAGCCCGTTTGCAGCATTTAAAATTGGAGTTGAATTTAACCTTGGCGCCAACATCTCAGCCATTGTTAATCCCGATTAttttt                                                                                                                >  1:419346/1‑250 (MQ=255)                                                                                                                                   gCCCACAAAACCTTGTGGACGGCGTTAAAAAATCAGCCTCAAACGGAAATGACCATAGCGGAAGTCAGCAAACCCAATACCGATTTTTTACACGCGCTTGCGCTTATTAACGACGAGGCATTATTGTTACAGCAAGGACAGCATTTTTATTTGTTGTCATTAGCCCGTTTGCAGCATTTAAAATTGGAGTTGAATTTAACCTTGGCGCCAACATCTCAGCCATTGTTAATCCCGATTATTTTTCGCTTAt                                                                                                         >  1:54475/1‑250 (MQ=255)                                                                                                                                    cccACAAAACCTTGTGGACGGCGTTAAAAAATCAGCCTCAAACGGAAATGACCATAGCGGAAGTCAGCAAACCCAATACCGATTTTTTACACGCGCTTGCGCTTATTAacga                                                                                                                                                                                                                                                  <  1:173865/112‑1 (MQ=255)                                                                                                                                          aaaCCTTGTGGACGGCGTTAAAAAATCAGCCTCAAACGGAAATGACCATAGCGGAAGTCAGCAAACCCAATACCGATTTTTTACACGCGCTTGCGCTTATTAACGACGAGGCATTATTGTTACAGCAAGGACAGCATTTTTATTTGTTGTCATTAGCCCGTTTGCAGCATTTAAAATTGGAGTTGAATTTAACCTTGGCGCCAACATCTCAGCCATTGTTAATCCCGATTATTTTTCGCTTATCTAATGAc                                                                                                 <  1:96738/251‑1 (MQ=255)                                                                                                                                          aaaCCTTGTGGACGGCGTTAAAAAATCAGCCTCAAACGGAAATGACCATAGCGGAAGTCAGCAAACCCAATACCGATTTTTTACACGCGCTTGCGCTTATTAACGACGAGGCATTATTGTTACAGCAAGGACAGCATTTTTATTTGTTGTCATTAGCCCGTTTGCAGCATTTAAAATTGGAGTTGAATTTAACCTTGGCGCCAACATCTCAGCCATTGTTAATCCCGATTATTTTTCGCTTATCTAATGAc                                                                                                 <  1:318548/251‑1 (MQ=255)                                                                                                                                            aCCTTGTGGACTGCGTTAAAAAATCAGCCTCAAACGGAAATGACCATAGCGGAAGTCAGCAAACCCAATACCGATTTTTTACACGCGCTCGCGCTTATTAacga                                                                                                                                                                                                                                                  >  1:453867/1‑104 (MQ=255)                                                                                                                                            aCCTTGTGGACGGCGTTAAAAAATCAGCCTCAAACGGAAATGACCATAGCGGAAGTCAGCAAACCCAATACCGATTTTTTACACGCGCTTGCGCTTATTAACGACGAGGCATTATTGTTACAGCAAGGAc                                                                                                                                                                                                                        >  1:560468/1‑130 (MQ=255)                                                                                                                                            aCCTTGTGGACGGCGTTAAAAAATCAGCCTCAAACGGAAATGACCATAGCGGAAGTCAGCAAACCCAATACCGATTTTTTACACGCGCTTGCGCTTATTAACGACGAGGCATTATTGTTACAGCAAGGACAGCATTTTTATTTGTTGTCATTAGCCCGTTTGCAGCATTTAAAATTGGAGTTGAATTTAACCTTGGCGCCAACATCTCAGCCATTGtt                                                                                                                                >  1:297474/1‑218 (MQ=255)                                                                                                                                            aCCTTGTGGACGGCGTTAAAAAATCAGCCTCAAACGGAAATGACCATAGCGGAAGTCAGCAAACCCAATACCGATTTTTTACACGCGCTTGCGCTTATTAACGACGAGGCATTATTGTTACAGCAAGGACAGCATTTTTATTTGTTGTCATTAGCCCGTTTGCAGCATTTAAAATTGGAGTTGAATTTAACCTTGGCGCCAACATCTCAGCCATTGTTAATCCCGATTATTTTTCGCTTATCTAATGACCa                                                                                               >  1:43528/1‑251 (MQ=255)                                                                                                                                              cTTGTGGACGGCGTTAAAAAATCAGCCTCAAACGGAAATGACCATAGCGGAAGTCAGCAAACCCAATACCGATTTTTTACACGCGCTTGCGCTTATTAACGACGAGGCATTATTGTTACAGCAAGGACAGCATTTTTATTTGTTGTCATTAGCCCGTTTGCAGCATTTAAAATTGGAGTTGAATTTAACCTTGGCGCCAACATCTCAGCCATTGTTAATCCCGATTATTTTTCGCTTATCTAATGACCaa                                                                                              >  1:149332/1‑250 (MQ=255)                                                                                                                                              cTTGTGGACGGCGTTAAAAAATCAGCCTCAAACGGAAATGACCATAGCGGAAGTCAGCAAACCCAATACCGATTTTTTACACGCGCTTGCGCTTATTAACGACGAGGCATTATTGTTACAGCAAGGACAGCATTTTTATTTGTTGTCATTAGCCCGTTTGCAGCATTTAAAATTGGAGTTGAATTTAACCTTGGCGCCAACATCTCAGCCATTGTTAATCCCGATTATTTTTCGCTTATCTAATGACCAAt                                                                                             >  1:707331/1‑251 (MQ=255)                                                                                                                                                tgtgGACGGCGTTAAAAAATCAGCCTCAAACGGAAATGACCATAGCGGAAGTCAGCAAACCCAATACCGATTTTTTACACGCGCTTGCGCTTATTAACGACGAGGCATTATTGTTACAGCAAGGACAGCATTTTTATTTGTTGTCATTAGCCCGTTTGCAGCATTTAAAATTGGAGTTGAATTTAACCTTGGCGCCAACATCTCAGCCATTGTTAATCCCGATTATTTTTCGCTTATCTAATGACCAATgg                                                                                           <  1:781008/251‑1 (MQ=255)                                                                                                                                                        gCGTTAAAAAATCAGCCTCAAACGGAAATGACCATAGCGGAAGTCAGCAAACCCAATACCGATTTTTTACACGCGCTTGCGCTTATTAACGACGAGGCATTATTg                                                                                                                                                                                                                                     >  1:828896/1‑105 (MQ=255)                                                                                                                                                        gCGTTAAAAAATCAGCCTCAAACGGAAATGACCATAGCGGAAGTCAGCAAACCCAATACCGATTTTTTACACGCGCTTGCGCTTATTAACGACGAGGCATTATTGTTACAGCAAGGACAGCATTTTTATTTGTTGTCATTAGCCCGTTTGCAGCATTTAAAATTGGAGTTGAATTTAACCTTGGCGCCAACATCTCAGCCATTGTTAATCCCGATTATTTTTCGCTTATCTAATGACCAATggc                                                                                          >  1:51587/1‑244 (MQ=255)                                                                                                                                                        gCGTTAAAAAATCAGCCTCAAACGGAAATGACCATAGCGGAAGTCAGCAAACCCAATACCGATTTTTTACACGCGCTTGCGCTTATTAACGACGAGGCATTATTGTTACAGCAAGGACAGCATTTTTATTTGTTGTCATTAGCCCGTTTGCAGCATTTAAAATTGGAGTTGAATTTAACCTTGGCGCCAACATCTCAGCCATTGTTAATCCCGATTATTTTTCGCTTATCTAATGACCAATggc                                                                                          >  1:857562/1‑244 (MQ=255)                                                                                                                                                           ttAAAAAATCAGCCTCAAACGGAAATGACCATAGCGGAAGTCAGCAAACCCAATACCGATTTTTTACACGCGCTTGCGCTTATTAACGACGAGGCATTATTGTTACAGCAAGGACAGCATTTTTATTTGTTGTCATTAGCCCGTTTGCAGCATTTAAAATTGGAGTTGAATTTAACCTTGGCGCCAACATCTCAGCCATTGTTAATCCCGATTATTTTTCGCTTATCTAATGACCAATGGCAGGCATGGtt                                                                                <  1:134261/251‑1 (MQ=255)                                                                                                                                                            tAAAAAATCAGCCTCAAACGGAAATGACCATAGCGGAAGTCAGCAAACCCAATACCGATTTTTTACACGCGCTTGCGCTTATTAACGACGAGGCATTATTGTTACAGCAAGGACAGCATTTTTATTTGTTGTCATTAGCCCGTTTGCAGCATTTAAAATTGGAGTTGAATTTAACCTTGGCGCCAACAtct                                                                                                                                           <  1:731814/191‑1 (MQ=255)                                                                                                                                                                aaaTCAGCCTCAAACGGAAATGACCATAGCGGAAGTCAGCAAACCCAATACCGATTTTTTACACGCGCTTGCGCTTATTAACGACGAGGCATTATTGTTACAGCAAGGACAGCATTTTTATTTGTTGTCATTAGCCCGTTTGCAGCATTTAAAATTGGAGTTGAATTTAACCTTGGCGCCAACATCTCAGCCATTGTTAATCCCGATTATTTTTCGCTTATCTAATGACCAATGGCAGGCATGGTTacaac                                                                           <  1:70840/251‑1 (MQ=255)                                                                                                                                                                aaaTCAGCCTCAAACGGAAATGACCATAGCGGAAGTCAGCAAACCCAATACCGATTTTTTACACGCGCTTGCGCTTATTAACGACGAGGCATTATTGTTACAGCAAGGACAGCATTTTTATTTGTTGTCATTAGCCCGTTTGCAGCATTTAAAATTGGAGTTGAATTTAACCTTGGCGCCAACATCTCAGCCATTGTTAATCCCGATTATTTTTCGCTTATCTAATGACCAATGGCAGGCATGGTTacaac                                                                           <  1:464791/251‑1 (MQ=255)                                                                                                                                                                                gAAATGACCATAGCGGAAGTCAGCAAACCCAATACCGATTTTTTACACGCGCTTGCGCTTATTAACGACGAGGCATTATTGTTACAGCAAGGACAGCATTTTTATTTGTTGTCATTAGCCCGTTTGCAGCATTTAAAATTGGAGTTGAATTTAACCTTGGCGCCAACATCTCAGCCATTGTTAATCCCGATTATTTTTCGCTTATCTAATGACCAATGGCAGGCATGGTTACAACAAAAAACGTTCTTTGa                                                           >  1:441757/1‑251 (MQ=255)                                                                                                                                                                                   aTGACCATAGCGGAAGTCAGCAAACCCAATACCGATTTTTTACACGCGCTTGCGCTTATTAACGACGAGGCATTATTGTTACAGCAAGGACAGCATTTTTATTTGTTGTCATTAGCCCGTTTGCAGCATTTAAAATTGGAGTTGAATTTAACCTTGGCGCCAACATCTCAGCCATTGTTAATCCCGATTATTTTTCGCTTATCTAATGACCAATGGCAGGCATGGTTACAACAAAAAACGTTCTTTGAGCa                                                        >  1:206750/1‑251 (MQ=255)                                                                                                                                                                                       ccATAGCGGAAGTCAGCAAACCCAATACCGATTTTTTACACGCGCTTGCGCTTATTAACGACGAGGCATTATTGTTACAGCAAGGACAGCATTTTTATTTGTTGTCATTAGCCCGTTTGCAGCATTTAAAATTGGAGTTGAATTTAACCTTGGCGCCAACATCTCAGCCATTGTTAATCCCGATTATTTTTCGCTTATCTAATGAcc                                                                                                <  1:156036/207‑1 (MQ=255)                                                                                                                                                                                       ccATAGCGGAAGTCAGCAAACCCAATACCGATTTTTTACACGCGCTTGCGCTTATTAACGACGAGGCATTATTGTTACAGCAAGGACAGCATTTTTATTTGTTGTCATTAGCCCGTTTGCAGCATTTAAAATTGGAGTTGAATTTAACCTTGGCGCCAACATCTCAGCCATTGTTAATCCCGATTATTTTTCGCTTATCTAATGACCAATGGCAGGCATGGTTACAACAAAAAACGTTCTTTGAGCAAAt                                                     >  1:817648/1‑250 (MQ=255)                                                                                                                                                                                          tAGCGGAAGTCAGCAAACCCAATACCGATTTTTTACACGCGCTTGCGCTTATTAACGACGAGGCATTATTGTTACAGCAAGGACAGCATTTTTATTTGTTGTCATTAGCCCGTTTGCAGCATTTAAAATTGGAGTTGAATTTAACCTTGGCGCCAACATCTCAGCCATTGTTAATCCCGATTATTTTTCGCTTATCTAATGACCAATGGCAGGCATGGTTACAACaaaaa                                                                      >  1:242047/1‑230 (MQ=255)                                                                                                                                                                                                    cAGCAAACCCAATACCGATTTTTTACACGCGCTTGCGCTTATTAACGACGAGGCATTATTGTTACAGCAAGGACAGCATTTTTATTTGTTGTCATTAGCCCGTTTGCAGCATTTAAAATTGGAGTTGAATTTAACCTTGGCGCCAACATCTCAGCCATTGTTAATCCCGATTATTTTTCGCTTATCTAATGACCAATGGCAGGCATGGTTac                                                                              >  1:888345/1‑212 (MQ=255)                                                                                                                                                                                                         aaCCCAATACCGATTTTTTACACGCGCTTGCGCTTATTAACGACGAGGCATTATTGTTACAGCAAGGACAGCATTTTTATTTGTTGTCATTAGCCCGTTTGCAGCATTTAAAATTGGAGTTGAATTTAACCTTGGCGCCAACATCTCAGCCATTGTTAATCCCGATTATTTTTCGCTTATCTAATGACCAATGGCAGGCATGGTTACAACAAAAAACGTTCTTTGAGCAAATCGGTTTTACTTTTACGGaa                                  >  1:400025/1‑251 (MQ=255)                                                                                                                                                                                                          aCCCAATACCGATTTTTTACACGCGCTTGCGCTTATTAACGACGAGGCATTATTGTTACAGCAAGGACAGCATTTTTATTTGTTGTCATTAGCCCGTTTGCAGCATTTAAAATTGGAGTTGAATTTAACCTTGGCGCCAACATCTCAGCCATTGTTAATCCCGATTATTTTTCGCTTATCTAATGACCAATGGCAGGCATGGTTACAACAAAAAACGTTCTTTGAGCAAATc                                                    >  1:333251/1‑232 (MQ=255)                                                                                                                                                                                                                           tACACGCGCTTGCGCTTATTAACGACGAGGCATTATTGTTACAGCAAGGACAGCATTTTTATTTGTTGTCATTAGCCCGTTTGCAGCATTTAAAATTGGAGTTGAATTTAACCTTGGCGCCAACATCTCAGCCATTGTTAATCCCGATTATTTTTCGCTTATCTAATGACCAATGGCAGGCATGGTTACAACAAAAAACGTTCTTTGAGCAAATCGGTTTTACTTTTACGGAAGAGGCGACACAACATaaa                >  1:668716/1‑251 (MQ=255)                                                                                                                                                                                                                            acacGCGCTTGCGCTTATTAACGACGAGGCATTATTGTTACAGCAAGGACAGCATTTTTATTTGTTGTCATTAGCCCGTTTGCAGCATTTAAAATTGGAGTTGAATTTAACCTTGGCGCCAACATCTCAGCCATTGTTAATCCCGATTATTTTTCGCTTATCTAATGACCAATGGCAGGCATGGTTACAACAAAAAACGTTCTTTGAGCAAATc                                                    <  1:605994/214‑1 (MQ=255)                                                                                                                                                                                                                             cacGCGCTTGCGCTTATTAACGACGAGGCATTATTGTTACAGCAAGGACAGCATTTTTATTTGTTGTCATTAGCCCGTTTGCAGCATTTAAAATTGGAGTTGAATTTAACCTTGGCGCCAACATCTCAGCCATTGTTAATCCCGATTATTTTTCGCTTATCTAATGACCAATGGCAGGCATGGTTACAACAAAAAACGTTCTTTGAGCAAATCGGTTTTACTTTTACGGAAGAGGCGACACAACATAAAAt              <  1:684765/251‑1 (MQ=255)                                                                                                                                                                                                                                 cgcttgcgcttATTAACGACGAGGCATTATTGTTACAGCAAGGACAGCATTTTTATTTGTTGTCATTAGCCCGTTTGCAGCATTTAAAATTGGAGTTGAATTTAACCTTGGCGCCAACATCTCAGCCATTGTTAATCCCGATTATTTTTCGCTTATCTAATGACCAATGGCAGGCATGGTTACAACAAAAAACGTTCTTTGAGCAAATCGGTTTTACTTTTACGGAAGAGGCGACACAACATAAAATAACg          >  1:726991/1‑251 (MQ=255)                                                                                                                                                                                                                                   cttgcgcttATTAACGACGAGGCATTATTGTTACAGCAAGGACAGCATTTTTATTTGTTGTCATTAGCCCGTTTGCAGCATTTAAAATTGGAGTTGAATTTAACCTTGGCGCCAACATCTCAGCCATTGTTAATCCCGATTATTTTTCGCTTATCTAATGACCAATGGCAGGCATGGTTACAACAAAAAACGTTCTTTGAGCAAATCGGTTTTACTTTTACGGAAGAGGCGACACAACATAAAATAACGc         <  1:160038/250‑1 (MQ=255)                                                                                                                                                                                                                                   cttgcgcttATTAACGACGAGGCATTATTGTTACAGCAAGGACAGCATTTTTATTTGTTGTCATTAGCCCGTTTGCAGCATTTAAAATTGGAGTTGAATTTAACCTTGGCGCCAACATCTCAGCCATTGTTAATCCCGATTATTTTTCGCTTATCTAATGACCAATGGCAGGCATGGTTACAACAAAAAACGTTCTTTGAGCAAATCGGTTTTACTTTTACGGAAGAGGCGACACAACATAAAATAACGc         <  1:51356/250‑1 (MQ=255)                                                                                                                                                                                                                                         cttATTAACGACGAGGCATTATTGTTACAGCAAGGACAGCATTTTTATTTGTTGTCATTAGCCCGTTTGCAGCATTTAAAATTGGAGTTGAATTTAACCTTGGCGCCAACAtc                                                                                                                                            >  1:844905/1‑113 (MQ=255)                                                                                                                                                                                                                                         cttATTAACGACGAGGCATTATTGTTACAGCAAGGACAGCATTTTTATTTGTTGTCATTAGCCCGTTTGCAGCATTTAAAATTGGAGTTGAATTTAACCTTGGCGCCAACATCTCAGCCATTGTTAATCCCGATTATTTTTCGCTTATCTAATGACCAATGGCAGGCATGGTTACAACAAAAAACGTTCTTTGAGCAAATCGGTTTTACTTTTACGGAAGAGGCGACACAACATAAAAt              <  1:515226/239‑1 (MQ=255)                                                                                                                                                                                                                                         cttATTAACGACGAGGCATTATTGTTACAGCAAGGACAGCATTTTTATTTGTTGTCATTAGCCCGTTTGCAGCATTTAAAATTGGAGTTGAATTTAACCTTGGCGCCAACATCTCAGCCATTGTTAATCCCGATTATTTTTCGCTTATCTAATGACCAATGGCAGGCATGGTTACAACAAAAAACGTTCTTTGAGCAAATCGGTTTTACTTTTACGGAAGAGGCGACACAACATAAAATAACGCTGCaaaa  >  1:843278/1‑251 (MQ=255)                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                                      GACTACCGGTTATTCCGCCACTGCCTACGCCGAAAAAAAGAATGACGGCACGCAAAAACACCGGCAAAGTTCACCGCACTTTTATCGTGAAAATGCCCATCGTGAACAAATTACGCAGAACGTATTGAATGCCCACAAAACCTTGTGGACGGCGTTAAAAAATCAGCCTCAAACGGAAATGACCATAGCGGAAGTCAGCAAACCCAATACCGATTTTTTACACGCGCTTGCGCTTATCAACGACGAGGCATTATTGTTACAGCAAGGACAGCATTTTTATTTGTTGTCATTAGCCCGTTTGCAGCATTTAAAATTGGAGTTGAATTTAACCTTGGCGCCAACATCTCAGCCATTGTTAATCCCGATTATTTTTCGCTTATCTAATGACCAATGGCAGGCATGGTTACAACAAAAAACGTTCTTTGAGCAAATCGGTTTTACTTTTACGGAAGAGGCGACACAACATAAAATAACGCTGCAAAA  >  1/1652201‑1652683

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑