Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,649,523	G→A	intergenic (+7684/+14852)	unknown → / ← unknown	tRNA‑Gly‑GCC/LSU rRNA ## 23S rRNA, large subunit ribosomal RNA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,649,523	0	G	A	100.0%	97.3 / NA	37	intergenic (+7684/+14852)	unknown/unknown	tRNA‑Gly‑GCC/LSU rRNA ## 23S rRNA, large subunit ribosomal RNA
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (20/17);  total (20/17)

GCTTTGGGGTATCAAGGTAAAGTAAAAAGCCCGACTTACACGTTAGTGGAAGAATATCGATTCCGCAACAAAACCGTTTACCATTTTGATTTGTATCGTCTTGCCGATCCTGAAGAGCTGGAATTTATGGGCATTCGTGATTATTTTAGTGAAAACACGCTTTGCTTGATTGAGTGGGCTGAGAAAGGAACAGGCATGCTTATGGCGGCAGATTTGTTGGTCAACATTGCCTATGCGGAAACTGCCCGTCACATTGAATTGGTTGCTCAATCGCCAATCGGCAGACAGATTATTGAACAATTAAATAACGATGATGTACCACAATGAAACTCGGCAATATCTTTAAATTATTTCTTGTCAACAGCGTTTTTTTCTTTGCAATGCCCGTTCTCGCCGGTAACGCATGGACAATTGCCGTTGATCCCGGTCATGGCGGGAAAGACCCCGGCGCTATCGGGCGCAACCTAAAAATTTATGAAAAAAA  >  1/1649276‑1649759                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                             gCTTTGGGGTATCAAGGTAAAGTAAAAAGCCCGACTTACACGTTAGTGGAAGAATATCGATTCCGCAACAAAACCGTTTACCATTTTGATTTGTATCGTCTTGCCGATCCTGAAGAGCTGGAATTTATGGGCATTCGTGATTATTTTAGTGAAAACACGCTTTGCTTGATTGAGTGGGCTGAGAAAGGAACAGGCATGCTTATGGCGGCAGATTTGTTGGTCAACATTGCCTATGCGGAAACTGCCCATca                                                                                                                                                                                                                                           >  1:754433/1‑251 (MQ=255)  cTTTGGGGTATCAAGGTAAAGTAAAAAGCCCGACTTACACGTTAGTGGAAGAATATCGATTCCGCAACAAAACCGTTTACCATTTTGATTTGTATCGTCTTGCCGATCCTGAAGAGCTGGAATTTATGGGCATTCGTGATTATTTTAGTGAAAACACGCTTTGCTTGATTGAGTGGGCTGAGAAAGGAACAGGCATGCTTATGGCGGCAGATTTGTTGGTCAACATTGCCTATGCGGAAACTGCCCATcac                                                                                                                                                                                                                                          <  1:67017/251‑1 (MQ=255)                                  aCTTACACGTTAGTGGAAGAATATCGATTCCGCAACAAAACCGTTTACCATTTTGATTTGTATCGTCTTGCCGATCCTGAAGAGCTGGAATTTATGGGCATTCGTGATTATTTTAGTGAAAACACGCTTTGCTTGATTGAGTGGGCTGAGAAAGGAACAGGCATGCTTATGGCGGCAGATTTGTTGGTCAACATTGCCTATGCGGAAACTGCCCATCACATTGAATTGGTTGCTc                                                                                                                                                                                                                          <  1:676121/235‑1 (MQ=255)                                  aCTTACACGGTAGTGGAAGAATATCGATTCCGCAACAAAACCGTTTACCATTTTGATTTGTATCGTCTTGCCGATCCTGAAGAGCTGGAATTTATGGGCATTCGTGATTATTTTAGTGAAAACACGCTTTGCTTGATTGAGTGGGCTGAGAAAGGAACAGGCATGCTTATGGCGGCAGATTTGTTGGTCAACATTGCCTATGCGGTAACTGCCCATCACAtgtaat                                                                                                                                                                                                                                   >  1:292160/1‑221 (MQ=255)                                    ttACACGTTAGTGGAAGAATATCGATTCCGCAACAAAACCGTTTACCATTTTGATTTGTATCGTCTTGCCGATCCTGAAGAGCTGGAATTTATGGGCATTCGTGATTATTTTAGTGAAAACACGCTTTGCTTGATTGAGTGGGCTGAGAAAGGAACAGGCATGCTTATGGCGGCAGATTTGTTGGTCAACATTGCCTATGCGGAAACTGCCCATCACATTGAATTGGTTGCTCAATCGCCAATCGGcagac                                                                                                                                                                                                        <  1:586922/251‑1 (MQ=255)                                           ttAGTGGAAGAATATCGATTCCGCAACAAAACCGTTTACCATTTTGATTTGTATCGTCTTGCCGATCCTGAAGAGCTGGAATTTATGGGCATTCGTGATTATTTTAGTGAAAACACGCTTTGCTTGATTGAGTGGGCTGAGAAAGGAACAGGCATGCTTATGGCGGCAGATTTGTTGGTCAACATTGCCTATGCGGAAACTGCCCATCACATTGAATTGGTTGCTCAATCGCCAATCGGCAGACAGattat                                                                                                                                                                                                 >  1:329804/1‑251 (MQ=255)                                                ggAAGAATATCGATTCCGCAACAAAACCGTTTACCATTTTGATTTGTATCGTCTTGCCGATCCTGAAGAGCTGGAATTTATGGGCATTCGTGATTATTTTAGTGAAAACACGCTTTGCTTGATTGAGTGGGCTGAGAAAGGAACAGGCATGCTTATGGCGGCAGATTTGTTGGTCAACATTGCCTATGCGGAAACTGCCCATCACATTGAATTGGTTGCTCAATCGCCAATCGGCAGACAGATTATTGaa                                                                                                                                                                                             >  1:689330/1‑250 (MQ=255)                                                 gaagaaTATCGATTCCGCAACAAAACCGTTTACCATTTTGATTTGTATCGTCTTGCCGATCCTGAAGAGCTGGAATTTATGGGCATTCGTGATTATTTTAGTGAAAACACGCTTTGCTTGATTGAGTGGGCTGAGAAAGGAACAGGCATGCTTATGGCGGCAGATTTGTTGGTCAACATTGCCTATGCGGAAACTGCCCATCACATTGAATTGGTTGCTCAATCGCCAATCGGCAGACAGATTATTGAAc                                                                                                                                                                                            <  1:160369/250‑1 (MQ=255)                                                     aaTATCGATTCCGCAACAAAACCGTTTACCATTTTGATTTGTATCGTCTTGCCGATCCTGAAGAGCTGGAATTTATGGGATTTCGTGATAATTTTAGTAAAAACACGCTTTGCTTGATTAAGTGGCCTGAAAAAGGACCAGGCATGCTTATGGCGGCAGTTTTGTTGGTCAACATTGCCTATGCGGAAACTCCCCATCACATTGAATTGGTTGCTCAATTGCCAACCGGCAGAACGATTATTGAACAATTa                                                                                                                                                                                       >  1:900895/1‑251 (MQ=255)                                                              tCCGCAACAAAACCGTTTACCATTTTGATTTGTATCGTCTTGCCGATCCTGAAGAGCTGGAATTTATGGGCATTCGTGATTATTTTAGTGAAAACACGCTTTGCTTGATTGAGTGGGCTGAGAAAGGAACAGGCATGCTTATGGCGGCAGATTTGTTGGTCAACATTGCCTATGCGGAAACTGCCCATCACATTGAATTGGTTGCTCAATCGCCAATCGGCAGACAGATTATTGAACAATTAAATAACgat                                                                                                                                                                              <  1:893438/251‑1 (MQ=255)                                                                    acaaAACCGTTTACCATTTTGATTTGTATCGTCTTGCCGATCCTGAAGAGCTGGAATTTATGGGCATTCGTGATTATTTTAGTGAAAACACGCTTTGCTTGATTGAGTGGGCTGAGAAAGGAACAGGCATGCTTATGGCGGCAGATTTGTTGGTCAACATTGCCTATGCGGAAACTGCCCATCACATTGAATTGGTTGCTCAATCGCCAATCGGCAGACAGATTATTGAACAATTAAATAACGATGATGTa                                                                                                                                                                        >  1:307163/1‑251 (MQ=255)                                                                          ccGTTTACCATTTTGATTTGTATCGTCTTGCCGATCCTGAAGAGCTGGAATTTATGGGCATTCGTGATTATTTTAGTGAAAACACGCTTTGCTTGATTGAGTGGGCTGAGAAAGGAACAGGCATGCTTATGGCGGCAGATTTGTTGGTCAACATTGCCTATGCGGAAACTGCCCATCACATTGAATTGGTTGCTCAATCGCCAATCGGCAGACAGATTATTGAACAATTAAATaa                                                                                                                                                                                  <  1:308945/235‑1 (MQ=255)                                                                                aCCATTTTGATTTGTATCGTCTTGCCGATCCTGAAGAGCTGGAATTTATGGGCATTCGTGATTATTTTAGTGAAAACACGCTTTGCTTGATTGAGTGGGCTGAGAAAGGAACAGGCATGCTTATGGCGGCAGATTTGTTGGTCAACATTGCCTATGCGGAAACTGCCCATCACATTGAATTGGTTGCTCAATCGCCAATCGGCAGACAGATTATTGAACAATTAAATAACGATGATGTACCACAATGAAAc                                                                                                                                                            <  1:311983/251‑1 (MQ=255)                                                                                                           aTCCTGAAGAGCTGGAATTTATGGGCATTCGTGATTATTTTAGTGAAAACACGCTTTGCTTGATTGAGTGGGCTGAGAAAGGAACAGGCATGCTTATGGCGGCAGATTTGTTGGTCAACATTGCCTATGCGGAAACTGCCCATcac                                                                                                                                                                                                                                          >  1:290513/1‑146 (MQ=255)                                                                                                           aTCCTGAAGAGCTGGAATTTATGGGCATTCGTGATTATTTTAGTGAAAACACGCTTTGCTTGATTGAGTGGGCTGAGAAAGGAACAGGCATGCTTATGGCGGCAGATTTGTTGGTCAACATTGCCTATGCGGAAACTGCCCATCACATTGAAtt                                                                                                                                                                                                                                  <  1:591865/154‑1 (MQ=255)                                                                                                           aTCCTGAAGAGCTGGAATTTATGGGCATTCGTGATTATTTTAGTGAAAACACGCTTTGCTTGATTGAGTGGGCTGAGAAAGGAACAGGCATGCTTATGGCGGCAGATTTGTTGGTCAACATTGCCTATGCGGAAACTGCCCATCACATTGAATTGGTTGCTCAATCGCCAATCGGCAGACAGATTATTGAACAATTAAATAACGATGATGTACcac                                                                                                                                                                    >  1:646627/1‑216 (MQ=255)                                                                                                                         gAATTTATGGGCATTCGTGATTATTTTAGTGAAAACACGCTTTGCTTGATTGAGTGGGCTGAGAAAGGAACAGGCATGCTTATGGCGGCAGATTTGTTGGTCAACATTGCCTATGCGGAAACTGCCCATCACATTGAATTGGTTGCTCAATCGCCAATCGGCAGACAGATTATTGAACAATTAAATAACGATGATGTACcac                                                                                                                                                                    <  1:372953/202‑1 (MQ=255)                                                                                                                            tttATGGGCATTCGTGATTATTTTAGTGAAAACACGCTTTGCTTGATTGAGTGGGCTGAGAAAGGAACAGGCATGCTTATGGCGGCAGATTTGTTGGTCAACATTGCCTATGCGGAAACTGCCCATCACATTGAATTGGTTGCTCAATCGCCAATCGGCAGACAGATTATTGAACAATTAAATAACGATGATGTACCACAATGAAACTCGGCAATATCTTTAAATTATTTCTTGTCAACAGCGttttttt                                                                                                                 >  1:739086/1‑250 (MQ=255)                                                                                                                                             ttattTTAGTGAAAACACGCTTTGCTTGATTGAGTGGGCTGAGAAAGGAACAGGCATGCTTATGGCGGCAGATTTGTTGGTCAACATTGCCTATGCGGAAACTGCCCATCACATTGAATTGGTTGCTCAATCGCCAATCGGCAGACAGATTATTGAACAATTAAATAACGATGATGTACCACAATGAAACTCGGCAATATCTTTAAATTATTTCTTGTCAACAGCGTTTTTTTCTTTGCAATGCCCGtt                                                                                                 >  1:542601/1‑249 (MQ=255)                                                                                                                                                                  ttGCTTGATTGAGTGGGCTGAGAAAGGAACAGGCATGCTTATGGCGGCAGATTTGTTGGTCAACATTGCCTATGCGGAAACTGCCCATCACATTGAATTGGTTGCTCAATCGCCAATCGGCAGACAGATTATTg                                                                                                                                                                                               >  1:425498/1‑134 (MQ=255)                                                                                                                                                                  ttGCTTGATTGAGTGGGCTGAGAAAGGAACAGGCATGCTTATGGCGGCAGATTTGTTGGTCAACATTGCCTATGCGGAAACTGCCCATCACATTGAATTGGTTGCTCAATCGCCAATCGGCAGACAGATTATTGAACAATTAAATAACGATGATGTACCACAATGAAACTCGGCAATATCTTTAAATTATTTCTTGTCAACAGCGTTTTTTTCTTTGCAATGCCCGTtct                                                                                               >  1:149693/1‑230 (MQ=255)                                                                                                                                                                  ttGCTTGATTGAGTGGGCTGAGAAAGGAACAGGCATGCTTATGGCGGCAGATTTGTTGGTCAACATTGCCTATGCGGAAACTGCCCATCACATTGAATTGGTTGCTCAATCGCCAATCGGCAGACAGATTATTGAACAATTAAATAACGATGATGTACCACAATGAAACTCGGCAATATCTTTAAATTATTTCTTGTCAACAGCGTTTTTTTCTTTGCAATGCCCGTTCTCGCCGGTAACGCATGGACAAt                                                                          >  1:384555/1‑251 (MQ=255)                                                                                                                                                                  ttGCTTGATTGAGTGGGCTGAGAAAGGAACAGGCATGCTTATGGCGGCAGATTTGTTGGTCAACATTGCCTATGCGGAAACTGCCCATCACATTGAATTGGTTGCTCAATCGCCAATCGGCAGACAGATTATTGAACAATTAAATAACGATGATGTACCACAATGAAACTCGGCAATATCTTTAAATTATTTCTTGTCAACAGCGTTTTTTTCTTTGCAATGCCCGTTCTCGCCGGTAACGCATGGACAAt                                                                          >  1:224288/1‑251 (MQ=255)                                                                                                                                                                    gCTTGATTGAGTGGGCTGAGAAAGGAACAGGCATGCTTATGGCGGCAGATTTGTTGGTCAACATTGCCTATGCGGAAACTGCCCATCACATTGAATTGGTTGCTCAATCGCCAATCGGCAGACAGATTATTg                                                                                                                                                                                               <  1:570193/132‑1 (MQ=255)                                                                                                                                                                    gCTTGATTGAGTGGGCTGAGAAAGGAACAGGCATGCTTATGGCGGCAGATTTGTTGGTCAACATTGCCTATGCGGAAACTGCCCATCACATTGAATTGGTTGCTCAATCGCCAATCGGCAGACAGATTATTGAACAATTAAATAACGATGATGTACCACAATGAAACTCGGCAATATCTTTAAATTATTTCTTGTCAACAGCGTTTTTTTc                                                                                                                >  1:101324/1‑211 (MQ=255)                                                                                                                                                                         attgaGTGGGCTGAGAAAGGAACAGGCATGCTTATGGCGGCAGATTTGTTGGTCAACATTGCCTATGCGGAAACTGCCCATCACATTGAATTGGTTGCTCAATCGCCAATCGGCAGACAGATTATTGAACAATTAAATAACGATGATGTACCACAATGAAACTCGGCAATATCTTTAAATTATTTCTTGTCAACAGCGTTTTTTTCTTTGCAATGCCCGTTCTCGCCGGTaa                                                                                      >  1:655243/1‑232 (MQ=255)                                                                                                                                                                                   cTGAGAAAGGAACAGGCATGCTTATGGCGGCAGATTTGTTGGTCAACATTGCCTATGCGGAAACTGCCCATCACATTGAATTGGTTGCTCAATCGCCAATCGGCAGACAGATTATTGAACAATTAAATAACGATGATGTACCACAATGAAACTCGGCAATATCTTTAAATTATTTCTTGTCAACAGCGTTTTTTTCTTTGCAATGCCCGTTCTCGCCGGTAACGCATGGACAATTGCCGTTGATCCCGGTc                                                         <  1:571893/251‑1 (MQ=255)                                                                                                                                                                                   cTGAGAAAGGAACAGGCATGCTTATGGCGGCAGATTTGTTGGTCAACATTGCCTATGCGGAAACTGCCCATCACATTGAATTGGTTGCTCAATCGCCAATCGGCAGACAGATTATTGAACAATTAAATAACGATGATGTACCACAATGAAACTCGGCAATATCTTTAAATTATTTCTTGTCAACAGCGTTTTTTTCTTTGCAATGCCCGTTCTCGCCGGTAACGCATGGACAATTGCCGTTGATCCCGGTc                                                         <  1:633483/251‑1 (MQ=255)                                                                                                                                                                                     gagaAAGGAACAGGCATGCTTATGGCGGCAGATTTGTTGGTCAACATTGCCTATGCGGAAACTGCCCATCACATTGAATTGGTTGCTCAATCGCCAATCGGCAGACAGATTATTGAACAATTAAATAACGATGATGTACCACAATGAAACTCGGCAATATCTTTAAATTATTTCTTGTCAACAg                                                                                                                          <  1:849383/184‑1 (MQ=255)                                                                                                                                                                                        aaaGGAACAGGCATGCTTATGGCGGCAGATTTGTTGGTCAACATTGCCTATGCGGAAACTGCCCATCACATTGAATTGGTTGCTCAATCGCCAATCGGCAGACAGATTATTGAACAATTAAATAACGATGATGTACCACAATGAAACTCGGCAATATCTTTAAATTATTTCTTGTCAACAGCGTTTTTTTCTTTGCAATGCCCGTTCTCGCCGGTAACGCATGGACAATTGCCGTTGATCCCGGTCATGGc                                                    <  1:393368/251‑1 (MQ=255)                                                                                                                                                                                            gAACAGGCATGCTTATGGCGGCAGATTTGTTGGTCAACATTGCCTATGCGGAAACTGCCCATCACATTGAATTGGTTGCTCAATCGCCAATCGGCAGACAGATTATTGAACAATTAAATAACGATGATGTACCACAATGAAACTCGGCAATATCTTTAAATTATTTCTTGTCAACAGCGTTTTTTTCTTTGCAATGCCCGTTCTCGCCGGTAACGc                                                                                   >  1:467083/1‑216 (MQ=255)                                                                                                                                                                                                                 cAGATTTGTTGGTCAACATTGCCTATGCGGAAACTGCCCATCACATTGAATTGGTTGCTCAATCGCCAATCGGCAGACAGATTATTGAACAATTAAATAACGATGATGTACCACAATGAAACTCGGCAATATCTTTAAATTATTTCTTGTCAACAGCGTTTTTTTCTTTGCAATGCCCGTTCTCGCCGGTAACGCATGGACAATTGCCGTTGATCCCGGTCATGGCGGGAAAGACCCCGGCGCTATCggg                            <  1:678788/250‑1 (MQ=255)                                                                                                                                                                                                                   gATTTGTTGGTCAACATTGCCTATGCGGAAACTGCCCATCACATTGAATTGGTTGCTCAATCGCCAATCGGCAGACAGATTATTGAACAATTAAATAACGATGATGTACCACAATGAAACTCGGCAATATCTTTAAATTATTTCTTGTCAACAGCGTTTTTTTCTTTGCAATGCCCGTTCTCGCCGGTAACGCATGGACAATTGCCGTTGATCCCGGTCATGGCGGGAAAGACCCCGGCGCTATCGGgcg                          >  1:555339/1‑250 (MQ=255)                                                                                                                                                                                                                           ggTCAACATTGCCTATGCGGAAACTGCCCATCACATTGAATTGGTTGCTCAATCGCCAATCGGCAGACAGATTATTGAACAATTAAATAACGATGATGTACCACAATGAAACTCGGCAATATCTTTAAATTATTTCTTGTCAACAGCGTTTTTTTCTTTGCAATGCCCGTTCTCGCCGGTAACGCATGGAc                                                                             <  1:225649/191‑1 (MQ=255)                                                                                                                                                                                                                                aCATTGCCTATGCGGAAACTGCCCATCACATTGAATTGGTTGCTCAATCGCCAATCGGCAGACAGATTATTGAACAATTAAATAACGATGATGTACCACAATGAAACTCGGCAATATCTTTAAATTATTTCTTGTCAACAGCGTTTTTTTCTTTGCAATGCCCGTTCTCGCCGGTAACGCATGGACAATTGCCGTTGATCCCGGTCATGGCGGGAAAGACCCCGGCGCTATCGGGCGCAACCTaaaaa               >  1:592195/1‑248 (MQ=255)                                                                                                                                                                                                                                          tGCGGAAACTGCCCATCACATTGAATTGGTTGCTCAATCGCCAATCGGCAGACAGATTATTGAACAATTAAATAACGATGATGTACCACAATGAAACTCGGCAATATCTTTAAATTATTTCTTGTCAACAGCGTTTTTTTCTTTGCAATGCCCGTTCTCGCCGGTAACGCATGGACAATTGCCg                                                                     <  1:722313/184‑1 (MQ=255)                                                                                                                                                                                                                                          tGCGGAAACTGCCCATCACATTGAATTGGTTGCTCAATCGCCAATCGGCAGACAGATTATTGAACAATTAAATAACGATGATGTACCACAATGAAACTCGGCAATATCTTTAAATTATTTCTTGTCAACAGCGTTTTTTTCTTTGCAATGCCCGTTCTCGCCGGTAACGCATGGACAATTGCCGTTGATCCCGGTCATGGCGGGAAAGACCCCGGCGCTATCGGGCGCAACCTAAAAAATTATGaaaaaaa  >  1:739727/1‑251 (MQ=255)                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                             GCTTTGGGGTATCAAGGTAAAGTAAAAAGCCCGACTTACACGTTAGTGGAAGAATATCGATTCCGCAACAAAACCGTTTACCATTTTGATTTGTATCGTCTTGCCGATCCTGAAGAGCTGGAATTTATGGGCATTCGTGATTATTTTAGTGAAAACACGCTTTGCTTGATTGAGTGGGCTGAGAAAGGAACAGGCATGCTTATGGCGGCAGATTTGTTGGTCAACATTGCCTATGCGGAAACTGCCCGTCACATTGAATTGGTTGCTCAATCGCCAATCGGCAGACAGATTATTGAACAATTAAATAACGATGATGTACCACAATGAAACTCGGCAATATCTTTAAATTATTTCTTGTCAACAGCGTTTTTTTCTTTGCAATGCCCGTTCTCGCCGGTAACGCATGGACAATTGCCGTTGATCCCGGTCATGGCGGGAAAGACCCCGGCGCTATCGGGCGCAACCTAAAAATTTATGAAAAAAA  >  1/1649276‑1649759

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑