Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,648,762	G→A	intergenic (+6923/+15613)	unknown → / ← unknown	tRNA‑Gly‑GCC/LSU rRNA ## 23S rRNA, large subunit ribosomal RNA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,648,762	0	G	A	100.0%	35.9 / NA	15	intergenic (+6923/+15613)	unknown/unknown	tRNA‑Gly‑GCC/LSU rRNA ## 23S rRNA, large subunit ribosomal RNA
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (7/8);  total (7/8)

AGATTTATGACGAAAAATATAATAATAGCTACTATGCGACGAAAAAATTACTTAATAAAAACAATTTAGAAAATAAAATTATATTACCTCCTATTGATTTAGGTGCTGCCGTTATTGCTACAACATCCAATGCTTCTATTTCTGCGCCTTACCATAGAAATATAAGAGGAAATTCTGATACAATGCGGTTTTATACGTCAGTTAATACAGATGATGCAGAAAAATACTTGATTAGAAATAATGTGGATTATATTCTGATTGAGAAAAATTTTGCCAATGTTTTAGCCGATAAATATAAGGGGAAAGAAATCATGGCTCAAAAATTAATAGATAAGAAAGATATACCTGATTATCTTGAATTTATTGATGCCAATGAAAACACCAATATTATTTTATATAAATTTATTAAACCCGAACTCAAATAACTTAACTATAATGT  >  1/1648528‑1648966                                                                                                                                                                                                                                           |                                                                                                                                                                                                             agaTTTATGACGAAAAATATAATAATAGCTACTATGCGACGGAAAAATTACTTAATAAAAACAATTTAGAAAATAAAATTATATTACCTCCTATTGATTTAGGTGCTGCCGTTATTGCTACAACATCCAATGCTTCTATTTCTGCGCCTTACCACAGAAATATAAGAGGAAATTCTGATACAATGCGGTTTTATACGTCAGTTAATACAGATGATGCAGAAAAATACTTGATTAAAAATAATGTGGATtat                                                                                                                                                                                              >  1:527916/1‑251 (MQ=255)                                                       ataaAAACAATTTAGAAAATAAAATTATATTACCTCCTATTGATTTAGGTGCTGCCGTTATTGCTACAACATCCAATGCTTCTATTTCTGCGCCTTACCACAGAAATATAAGAGGAAATTCTGATACAATGCGGTTTTATACGTCAGTTAATACAGATGATGCAGAAAACTACTTGATTAAAAATAATGTGGATTATATTCTGATTGAGAAAAATTTTGCCAATGTTTTAGCCGATAAATATAAGGGgaaa                                                                                                                                        >  1:439739/1‑251 (MQ=255)                                                                                 atatTACCTCCTATTGATTTAGGTGCTGCCGTTATTGCTACAACATCCAATGCTTCTATTTCTGCGCCTTACCACAGAAATATAAGAGGAAATTCTGATACAATGCGGTTTTATACGTCAGTTAATACAGATGATGCAGAAAAATACTTGATTAAAAATAATGTGGATTATATTCTGATTGAGAAAAATTTTGCCAATGTTTTAGCCGATAAATATAAggg                                                                                                                                            <  1:139466/221‑1 (MQ=255)                                                                                      aCCTCCTATTGATTTAGGTGCTGCCGTTATTGCTACAACATCCAATGCTTCTATTTCTGCGCCTTACCACAGAAATATAAGAGGAAATTCTGATACAATGCGGTTTTATACGTCAGTTAATACAGATGATGCAGAAAAATACTTGATTAAAAATAATGTGGATTATATTCTGATTGAGAAAAATTTTGCCAATGTTTTAGCCGATAAATATAAggg                                                                                                                                            <  1:556961/216‑1 (MQ=255)                                                                                            tATTGATTTAGGTGCTGCCGTTATTGCTACAACATCCAATACTTCTATTTCTGCGCCTTACCACAGAAATATAAGAGGAAATTCTGATACAATGCGGTTTTATACGTCAGTTAATACAGATGATGCAGAAAAATACTTGATTAAAAATAATGTGGATTATATTCTGATTGAGAAAAATTTTGCCAATGTTTTAGCCGATAAATATAAGGGGAAAGAAATCATGGCTCAAAAATTAATAGATAAGAAAGata                                                                                                   >  1:273666/1‑251 (MQ=255)                                                                                                          ctgcCGTTATTGCTACAACATCCAATGCTTCTATTTCTGCGCCTTACCACAGAAATATAAGAGGAAATTCTGATACAATGCGGTTTTATACGTCAGTTAATACAGATGATGCAGAAAAATACTTGATTAAAAATAATGTGGATTATATTCTGATTGAGAAAAATTTTGCCAATGTTTTAGCCGATAAATATAAGGGGAAAGAAATCATGGCTCAAAAATTAATAGATAAGAAAGATATACCTGATTATCt                                                                                      <  1:99260/250‑1 (MQ=255)                                                                                                            gcCGTTATTGCTACAACATCCAATGCTTCTATTTCTGCGCCTTACCACAGAAATATAAGAGGAAATTCTGATACAATGCGGTTTTATACGTCAGTTAATACAGATGATGCAGAAAAATACTTGATTAAAAATAATGTGGATTATATTCTGATTGAGAAAAATTTTGCCAATGTTTTAGCCGATAAATATAAGGGGAAAGAAATCATGGCTCAAAAATTAATAGATAAGAAAGATATAcc                                                                                               <  1:546466/239‑1 (MQ=255)                                                                                                               gTTATTGCTACAACATCCAATGCTTCTATTTCTGCGCCTTACCACAGAAATATAAGAGGAAATTCTGATACAATGCGGTTTTATACGTCAGTTAATACAGATGATGCAGAAAAATACTTGATTAAAAATAATGTGGATTATATTCTGa                                                                                                                                                                                       >  1:139765/1‑148 (MQ=255)                                                                                                                                    gCTTCTATTTCTGCGCCTTACCACAGAAATATAAGAGGAAATTCTGATACAATGCGGTTTTATACGTCAGTTAATACAGATGATGCAGAAAAATACTTGATTAAAaat                                                                                                                                                                                                          >  1:721180/1‑108 (MQ=255)                                                                                                                                                                   taAGAGGAAATTCTGATACAATGCGGTTTTATACGTCAGTTAATACAGATGATGCAGAAAAATACTTGATTAAAAATAATGTGGATTATATTCTGATTGAGAAAAATTTTGCCAATGTTTTAGCCGATAAATATAAGGGGAAAGAAATCATGGCTCAAAAATTAATAGATAAGAAAGATATACCTGATTATCTTGAATTTATTGATGCCAATGAAAACAcc                                                          <  1:37990/221‑1 (MQ=255)                                                                                                                                                                             ttCTGATACAATGCGGTTTTATACGTCAGTTAATACAGATGATGCAGAAAAATACTTGATTAAAAATAATGTgg                                                                                                                                                                                                   <  1:689890/74‑1 (MQ=255)                                                                                                                                                                                                        aGTTAATACAGATGATGCAGAAAAATACTTGATTAAAAATAATGTgg                                                                                                                                                                                                   <  1:523466/47‑1 (MQ=255)                                                                                                                                                                                                         gTTAATACAGATGATGCAGAAAAATACTTGATTAAAAATAATGTGGATTATATTCTGATTGAGAAAAATTTTGCCAATGTTTTAGCCGATAAATATAAGGGGAAAGAAAt                                                                                                                                   <  1:310685/110‑1 (MQ=255)                                                                                                                                                                                                            aaTACAGATGATGCAGAAAAATACTTGATTAAAAATAATGTGGATTATATTCTGATTGAGAAAAATTTTGCCAATGTTTTAGCCGATAAATATAAGGGGAAAGAAATCATGGCTCAAAAATTAATAGATAAGAAAGATATAcc                                                                                               >  1:722431/1‑143 (MQ=255)                                                                                                                                                                                                                           gAAAAATACTTGATTAAAAATAATGTGGATTATATTCTGATTGAGAAAAATTTTGCCAATGTTTTAGCCGATAAATATAAGGGGAAAGAAATCATGGCTCAAAAATTAATAGATAAGAAAGATATACCTGATTATCTTGAATTTATTGATGCCAATGAAAACACCAATATTATTTTATATAAATTTATTAAACCCGAACTCAAATAACTTAACTAtaatgt  >  1:841356/1‑221 (MQ=255)                                                                                                                                                                                                                                           |                                                                                                                                                                                                             AGATTTATGACGAAAAATATAATAATAGCTACTATGCGACGAAAAAATTACTTAATAAAAACAATTTAGAAAATAAAATTATATTACCTCCTATTGATTTAGGTGCTGCCGTTATTGCTACAACATCCAATGCTTCTATTTCTGCGCCTTACCATAGAAATATAAGAGGAAATTCTGATACAATGCGGTTTTATACGTCAGTTAATACAGATGATGCAGAAAAATACTTGATTAGAAATAATGTGGATTATATTCTGATTGAGAAAAATTTTGCCAATGTTTTAGCCGATAAATATAAGGGGAAAGAAATCATGGCTCAAAAATTAATAGATAAGAAAGATATACCTGATTATCTTGAATTTATTGATGCCAATGAAAACACCAATATTATTTTATATAAATTTATTAAACCCGAACTCAAATAACTTAACTATAATGT  >  1/1648528‑1648966

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑