Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,648,569	A→G	intergenic (+6730/+15806)	unknown → / ← unknown	tRNA‑Gly‑GCC/LSU rRNA ## 23S rRNA, large subunit ribosomal RNA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,648,569	0	A	G	100.0%	31.0 / NA	13	intergenic (+6730/+15806)	unknown/unknown	tRNA‑Gly‑GCC/LSU rRNA ## 23S rRNA, large subunit ribosomal RNA
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (5/8);  total (5/8)

GCAATGTCTGTTGCAATTCCTTTACAAGCTTATACTTGCTACTCATTAAGAGAGAAATGTTCATTACCGATAACAAAAACTATCATTTTATTTTTATCAATACCTACATTTCTATTAATTTCAACTAAAATAATCATAAATTTCAACGATGAAAATGAATCCAAAGAGATTTATGACGAAAAATATAATAATAGCTACTATGCGACGAAAAAATTACTTAATAAAAACAATTTAGAAAATAAAATTATATTACCTCCTATTGATTTAGGTGCTGCCGTTATTGCTACAACATCCAATGCTTCTATTTCTGCGCCTTACCATAGAAATATAAGAGGAAATTCTGATACAATGCGGTTTTATACGTCAGTTAATACAGATGATGCAGAAAAATACTTGATTAGAAATAATGTGGATTAT  >  1/1648362‑1648778                                                                                                                                                                                                                |                                                                                                                                                                                                                  gCAATGTCTGTTGCAATTCCTTTACAAGCTTATACTTGCTACTCATTAAGAGAGAAATGTTCATTACCGATAACAAAAACTATCATTTTATTTTTATCAATACCTACATTTCTATTAATTTCAACTAAAATAATCATAAATTTCAACGATGAAAATGAATCCAAAGAGATTTATGACGAAAAATATAATAATAGCTACTATGCGACGGAAAAATTACTTAATAAAAACAATTTAGAAAATAAAATTATAtt                                                                                                                                                                        >  1:459835/1‑251 (MQ=255)                                            cATTAAGAGAGAAATGTTCATTACCGATAACAAAAACTATCATTTTATTTTTATCAATACCTACATTTCTATTAATTTCAACTAAAATAATCATAAATTTCAACGATGAAAATGAATCCAAAGAGATTTATGACGAAAAATATAATAATAGCTACTATGCGACGGAAAAATTACTTAATAAAAACAATTTAGAAAATAAAATTATATTACCTCCTATTGATTTAGGTGCTGCCGTTATTGCTACAACATcc                                                                                                                             <  1:414799/251‑1 (MQ=255)                                                                                  aTCATTTTATTTTTATCAATACCTACATTTCTATTAATTTCAACTAAAATAATCATAAATTTCAACGATGAAAATGAATCCAAAGAGATTTATGACGAAAAATATAATAATAGCTACTATGCGACGGAAAAATTACTTAATAAAAACAATTTAGAAAATAAAATTATATTACCTCCTATTGATTTAGGTGCTGCCGTTATTGCTACAACATCCAATGCTTCTATTTCTGCGCCTTACCACAGAAATATAag                                                                                       <  1:16505/251‑1 (MQ=255)                                                                                                 tCAATACCTACATTTCTATTAATTTCAACTAAAATAATCATAAATTTCAACGATGAAAATGAATCCAAAGAGATTTATGACGAAAAATATAATAATAGCTACTATGCGACGGAAAAATTACTTAATAAAAACAATTTAGAAAATAAAATTATATTACCTCCTATTGATTTAGGTGCTGCCGTTATTGCTACAACATCCAATGCTTCTATTTCTGCGCCTTACCACAGAAATATAAGAGGAAATTCTGATAc                                                                        <  1:438127/251‑1 (MQ=255)                                                                                                           cATTTCTATTAATTTCAACTAAAATAATCATAAATTTCAACGATGAAAATGAATCCAAAGAGATTTATGACGAAAAATATAATAATAGCTACTATGCGACGGAAAAATTACTTAATAAAAACAATTTAGAAAATAAAATTATATTACCTCCTATTGATTTAGGTGCTGCCGTTATTGc                                                                                                                                       <  1:487752/178‑1 (MQ=255)                                                                                                                  aTTAATTTCAACTAAAATAATCATAAATTTCAACGATGAAAATGAATCCAAAGAGATTTATGACGAAAAATATAATAATAGCTACTATGCGACGGAAAAATTACTTAATAAAAACAATTTAGAAAATAAAATTATATTACCTCCTATTGATTTAGGTGCTGCCGTTATTGCTACAACATCCAATGCTTCTATTTCTGCGCCTTACCACAGAAATATAAGAGGAAATTCTGATACAATGCGGTTTTATACGt                                                       >  1:336669/1‑251 (MQ=255)                                                                                                                          cAACTAAAATAATCATAAATTTCAACGATGAAAATGAATCCAAAGAGATTTATGACGAAAAATATAATAATAGCTACTATGCGACGGAAAAATTACTTAATAAAAACAATTTAGAAAATAAAATTATATTACCTCCTATTGATTTAGGTGCTGCCGTTATTGCTACAACATCCAATGCTTCTATTTCTGCGCCTTACCac                                                                                                  <  1:147249/200‑2 (MQ=255)                                                                                                                            aCTAAAATAATCATAAATTTCAACGATGAAAATGAATCCAAAGAGATTTATGACGAAAAATATAATAATAGCTACTATGCGACGGAAAAATTACTTAATAAAAACAATTTAGAAAATAAAATTATATTACCTCCTATTGATTTAGGTGCTGCCGTTATTGCTACAACATCCAATGCTTCTATTTCTGCGCCTTACCACAGAAATATAAGAGGAAATTCTGATACAATGCGGTTTTATACGTCAGTTAATAc                                             <  1:197881/251‑1 (MQ=255)                                                                                                                                       cATAAATTTCAACGATGAAAATGAATCCAAAGAGATTTATGACGAAAAATATAATAATAGCTACTATGCGACGGAAAAATTACTTAATAAAAACAATTTAGAAAATAAAATTATATTACCTCCTATTGATTTAGGTGCTGCCGTTATTGCTACAACATCCAATGCTTCTATTTCTGCGCCTTACCACAGAAATATAAGAGGAAATTCTGATA‑AATGCGGTTTTATACGTCAGTTAATAc                                             >  1:336391/1‑239 (MQ=255)                                                                                                                                        aTAAATTTCAACGATGAAAATGAATCCAAAGAGATTTATGACGAAAAATATAATAATAGCTACTATGCGACGGAAAAATTACTTAATAAAAACAATTTAGAAAATAAAATTATATTACCTCCTATTGATTTAGGTGCTGCCGTTATTGCTACAACATCCAATGCTTCTATTTCTGCGCCTTACCACAGAAATATAagag                                                                                     >  1:446569/1‑199 (MQ=255)                                                                                                                                                     aTGAAAATGAATCCAAAGAGATTTATGACGAAAAATATAATAATAGCTACTATGCGACGGAAAAATTACTTAATAAAAACAATTTAGAAAATAAAATTATATTAc                                                                                                                                                                      <  1:5463/105‑1 (MQ=255)                                                                                                                                                                       agaTTTATGACGAAAAATATAATAATAGCTACTATGCGACGGAAAAATTACTTAATAAAAACAATTTAGAAAATAAAATTATATTACCTCCTATTGATTTAGGTGCTGCCGTTATTGCTACAACATCCAATGCTTCTATTTCTGCGCCTTACCACAGAAATATAAGAGGAAATTCTGATACAATGCGGTTTTATACGTCAGTTAATACAGATGATGCAGAAAAATACTTGATTAAAAATAATGTGGATtat  >  1:527916/1‑251 (MQ=255)                                                                                                                                                                                                  gCTACTATGCGACGGAAAAATTACTTAATAAAAACAATTTAGAAAATAAAATTATATTACCTCCTATTGATTTAGGTGCTGCCGTTATTGCTACAACATCCAATGCTTCTATTTCTGCGCCTTACCACAGAAATATAAGAGGAAATTc                                                                              <  1:772459/148‑1 (MQ=255)                                                                                                                                                                                                                |                                                                                                                                                                                                                  GCAATGTCTGTTGCAATTCCTTTACAAGCTTATACTTGCTACTCATTAAGAGAGAAATGTTCATTACCGATAACAAAAACTATCATTTTATTTTTATCAATACCTACATTTCTATTAATTTCAACTAAAATAATCATAAATTTCAACGATGAAAATGAATCCAAAGAGATTTATGACGAAAAATATAATAATAGCTACTATGCGACGAAAAAATTACTTAATAAAAACAATTTAGAAAATAAAATTATATTACCTCCTATTGATTTAGGTGCTGCCGTTATTGCTACAACATCCAATGCTTCTATTTCTGCGCCTTACCATAGAAATATAAGAGGAAATTCTGATACAATGCGGTTTTATACGTCAGTTAATACAGATGATGCAGAAAAATACTTGATTAGAAATAATGTGGATTAT  >  1/1648362‑1648778

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑