Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,647,287	T→C	intergenic (+5448/+17088)	unknown → / ← unknown	tRNA‑Gly‑GCC/LSU rRNA ## 23S rRNA, large subunit ribosomal RNA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,647,287	0	T	C	100.0%	101.8 / NA	38	intergenic (+5448/+17088)	unknown/unknown	tRNA‑Gly‑GCC/LSU rRNA ## 23S rRNA, large subunit ribosomal RNA
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (24/14);  total (24/14)

TTCCGGTATAAGCCTAATCCTAGGTTTTTTATATTCCAGATATATTGTTTTTCGAAATAATAAGTAATAATTAAACACTAAAAAGGTTAATATGTATAATAAATTATTTATAAATAATGACATAAAAAAATTAGTTTATATCGCTTGTATTATTTGGATATTATGTGCCACTTTTTTAATTATACGGAACTGGAGTGATGTCGCAAATTTCGCATTGAGTGATACTGATGATCTTATGCGCTATCATCAATATAGTGAGTGGTTAAAAAATGGGAATTGGTATTTACTGCCGGATCCTCAATTTAACCCACAAGACGGCGTTATCATGCACTGGTCCAGAGTTGTGGATATTCCATTAGCCGGGGTAGCCCTGCTTGCTTCTATTTTCACCGATTGGAACACCGCATTCACCATATCGAATGTTATAACACCACTCCTTTATCTATTAATTTTTTTAT  >  1/1647068‑1647525                                                                                                                                                                                                                            |                                                                                                                                                                                                                                               ttCCGGTATAAGCCTAATCCTAGGTTTTTTATATTCCAGATATATTGTTTTTCGAAATAATAAGTAATAATTAAACACTAAAAAGGTTAATATGTATAATAAATTATTTATAAATAATAACATAAAAAAATTAGTTTATATCGCTTGTATTATTTGGATATTATGTGCCACTTTTTTAATTATACGGAACTGGAGTGATGTCGCAAATTTCGCATTGAGCGATACTGATGATCTTATGCGCTATCATCAat                                                                                                                                                                                                                 <  1:842868/251‑1 (MQ=255)                             ttATATTCCAGATATATTGTTTTTCGAAATAATAAGTAATAATTAAACACTAAAAAGGTTAATATGTATAATAAATTATTTATAAATAATAACATAAAAAAATTAGTTTATATCGCTTGTATTATTTGGATATTATGTGCCACTTTTTTAATTATACGGAACTGGAGTGATGTCGCAAATTTCGCATTGAGCGATACTGATGATCTTATGCGCTATCATCAATATAGTGAGTGGTTaaaaaa                                                                                                                                                                                              >  1:560144/1‑242 (MQ=255)                                               gTTTTTCGAAATAATAAGTAATAATTAAACACTAAAAAGGTTAATATGTATAATAAATTATTTATAAATAATAACATAAAAAAATTAGTTTATATCGCTTGTATTATTTGGATATTATGTGCCACTTTTTTAATTATACGGAACTGGAGTGATGTCGCAAATTTCGCATTGAGCGATACTGATGATCTTATGCGCTATCATCAATATAGTGAGTGGTTAAAAAATGGGAATTGGTATTTACTGCCGGATc                                                                                                                                                                    >  1:480259/1‑250 (MQ=255)                                                           aataaGTAATAATTAAACACTAAAAAGGTTAATATGTATAATAAATTATTTATAAATAATAACATAAAAAAATTAGTTTATATCGCTTGTATTATTTGGATATTATGTGCCACTTTTTTAATTATACGGAACTGGAGTGATGTCGCAAATTTCGCATTGAGCGATACTGATGATCTTATGCGCTATCATCAATATAGTGAGTGGTTAAAAAATGGGAATTGGTATTTACTGCCGGATCCTCAATTTAAcc                                                                                                                                                        >  1:291422/1‑250 (MQ=255)                                                                                 aaaaGGTTAATATGTATAATAAATTATTTATAAATAATAACATAAAAAAATTAGTTTATATCGCTTGTATTATTTGGATATTATGTGCCACTTTTTTAATTATACGGAACTGGAGTGATGTCGCAAATTTCGCATTGAGCGATACTGATGATCTTATGCGCTATCATCAATATAGTGAGTGGTTAAAAAATGGGAATTGGTATTTACTGCCGGATCCTCAATTTAACCCACAAGACGGCGTTATCATGCAc                                                                                                                                 <  1:252364/251‑1 (MQ=255)                                                                                      gTTAATATGTATAATAAATTATTTATAAATAATAACATAAAAAAATTAGTTTATATCGCTTGTATTATTTGGATATTATGTGCCACTTTTTTAATTATACGGAACTGGAGTGATGTCGCAAATTTCGCATTGAGCGATACTGATGATCTTATGCGCTATCATCAATATAGTGAGTGGTTAAAAAATGGGAATTGGTATTTACTGCCGGATCCTCAATTTAACCCACAAGACGGCGTTATCATGCACTGGt                                                                                                                             >  1:81424/1‑250 (MQ=255)                                                                                            atGTATAATAAATTATTTATAAATAATAACATAAAAAAATTAGTTTATATCGCTTGTATTATTTGGATATTATGTGCCACTTTTTTAATTATACGGAACTGGAGTGATGTCGCAAATTTCGCATTGAGCGATACTGATGATCTTATGCGCTATCATCAATATAGTGAGTGGTTAAAAAATGGGAATTGGTATTTACTGCCGGATCCTCAATTTAACCCACAAGACGGCGTTATCATGCaa                                                                                                                                 <  1:538508/240‑2 (MQ=255)                                                                                                                        aCATAAAAAAATTAGTTTATATCGCTTGTATTATTTGGATATTATGTGCCACTTTTTTAATTATACGGAACTGGAGTGATGTCGCAAATTTCGCATTGAGCGATACTGATGATCTTATGCGCTATCATCAat                                                                                                                                                                                                                 <  1:801285/132‑1 (MQ=255)                                                                                                                                 aaTTAGTTTATATCGCTTGTATTATTTGGATATTATGTGCCACTTTTTTAATTATACGGAACTGGAGTGATGTCGCAAATTTCGCATTGAGCGATACTGATGATCTTATGCGCTATCATCAATATAGTGAGTGGTTAAAAAATGGGAATTGGTATTTACTGCCGGATCCTCAATTTAACCCACAAGACGGCGTTATCATGCACTGGTCCAGAGTTGTGGATATTCCATTAGCCGGGGTAGCCCtgcttg                                                                                   >  1:580298/1‑249 (MQ=255)                                                                                                                                     aGTTTATATCGCTTGTATTATTTGGATATTATGTGCCACTTTTTTAATTATACGGAACTGGAGTGATGTCGCAAATTTCGCATTGAGCGATACTGATGATCTTATGCGCTATCATCAATATAGTGAGTGGTTAAAAAATGGGAATTGGTATTTACTGCCGGATCCTCAATTTAAcc                                                                                                                                                        >  1:898274/1‑176 (MQ=255)                                                                                                                                     aGTTTATATCGCTTGTATTATTTGGATATTATGTGCCACTTTTTTAATTATACGGAACTGGAGTGATGTCGCAAATTTCGCATTGAGCGATACTGATGATCTTATGCGCTATCATCAATATAGTGAGTGGTTAAAAAATGGGAATTGGTATTTACTGCCGGATCCTCAATTTAACCCACAAGACGGCGTTATCATGCAc                                                                                                                                 >  1:411281/1‑199 (MQ=255)                                                                                                                                     aGTTTATATCGCTTGTATTATTTGGATATTATGTGCCACTTTTTTAATTATACGGAACTGGAGTGATGTCGCAAATTTCGCATTGAGCGATACTGATGATCTTATGCGCTATCATCAATATAGTGAGTGGTTAAAAAATGGGAATTGGTATTTACTGCCGGATCCTCAATTTAACCCACAAGACGGCGTTATCATGCACTGGTCCAGAGt                                                                                                                      >  1:519896/1‑210 (MQ=255)                                                                                                                                     aGTTTATATCGCTTGTATTATTTGGATATTATGTGCCACTTTTTTAATTATACGGAACTGGAGTGATGTCGCAAATTTCGCATTGAGCGATACTGATGATCTTATGCGCTATCATCAATATAGTGAGTGGTTAAAAAATGGGAATTGGTATTTACTGCCGGATCCTCAATTTAACCCACAAGACGGCGTTATCATGCACTGGTCCAGAGTTGTGGATATTCCATTAGCCGGGGTAGCCCTGCTTGCTTCTa                                                                             >  1:851922/1‑251 (MQ=255)                                                                                                                                      gTTTATATCGCTTGTATTATTTGGATATTATGTGCCACTTTTTTAATTATACGGAACTGGAGTGATGTCGCAAATTTCGCATTGAGCGATACTGATGATCTTATGCGCTatca                                                                                                                                                                                                                      <  1:344144/113‑1 (MQ=255)                                                                                                                                           tatCGCTTGTATTATTTGGATATTATGTGCCACTTTTTTAATTATACGGAACTGGAGTGATGTCGCAAATTTCGCATTGAGCGATACTGATGATCTTATGCGCTATCATCAATATAGTGAGTGGTTAAAAAATGGGAATTGGTATTTACTGCCGGATCCTCAATTTAACCCACAAGACGGCGTTATCATGCACTGGTCCAGAGt                                                                                                                      >  1:254015/1‑204 (MQ=255)                                                                                                                                              cGCTTGTATTATTTGGATATTATGTGCCACTTTTTTAATTATACGGAACTGGAGTGATGTCGCAAATTTCGCATTGAGCGATACTGATGATCTTATGCGCTATCATCAATATAGTGAGTGGTTAAAAAATGGGAATTGGTATTTACTGCCGGATCCTCAATTTAACCCACAAGACGGCGTTATCa                                                                                                                                      >  1:284168/1‑185 (MQ=255)                                                                                                                                              cGCTTGTATTATTTGGATATTATGTGCCACTTTTTTAATTATACGGAACTGGAGTGATGTCGCAAATTTCGCATTGAGCGATACTGATGATCTTATGCGCTATCATCAATATAGTGAGTGGTTAAAAAATGGGAATTGGTATTTACTGCCGGATCCTCAATTTAACCCACAAGACGGCGTTATCATGCACTGGTCCAGAGt                                                                                                                      >  1:245950/1‑201 (MQ=255)                                                                                                                                              cGCTTGTATTATTTGGATATTATGTGCCACTTTTTTAATTATACGGAACTGGAGTGATGTCGCAAATTTCGCATTGAGCGATACTGATGATCTTATGCGCTATCATCAATATAGTGAGTGGTTAAAAAATGGGAATTGGTATTTACTGCCGGATCCTCAATTTAACCCACAAGACGGCGTTATCATGCACTGGTCCAGAGTTGTGGATATTCCATTAGCCGGGGTAGCCCTGCTTGCTTCTATTTTCACCg                                                                    >  1:312250/1‑251 (MQ=255)                                                                                                                                                cTTGTATTATTTGGATATTATGTGCCACTTTTTTAATTATACGGAACTGGAGTGATGTCGCAAATTTCGCATTGAGCGATACTGATGATCTTATGCGCTATCATCAATATAGTGAGTGGTTAAAAAATGGGAATTGGTGTTTACTGCCGGATCCTCAATTTAACCCACAAGACGGCGTTATCATGCACTGGTCCAGAGTTGTGGATATTCCATTAGCCGGGGTAGCCCTGCTTGCTTCTATTTTCACCGAt                                                                  >  1:566724/1‑251 (MQ=255)                                                                                                                                                cTTGTATTATTTGGATATTATGTGCCACTTTTTTAATTATACGGAACTGGAGTGATGTCGCAAATTTCGCATTGAGCGATACTGATGATCTTATGCGCTATCATCAATATAGTGAGTGGTTAAAAAATGGGAATTGGTATTTACTGCCGGATCCTCAATTTAACCCACAAGACGGCGTTATCATGCACTGGTCCagag                                                                                                                       >  1:551128/1‑198 (MQ=255)                                                                                                                                                   gTATTATTTGGATATTATGTGCCACTTTTTTAATTATACGGAACTGGAGTGATGTCGCAAATTTCGCATTGAGCGATACTGATGATc                                                                                                                                                                                                                                   >  1:31549/1‑87 (MQ=255)                                                                                                                                                   gTATTATTTGGATATTATGTGCCACTTTTTTAATTATACGGAACTGGAGTGATGTCGCAAATTTCGCATTGAGCGATACTGATGATCTTATGCGCTATCATCAATATAGTGAGTGGTTAAAAAATGGGAATTGGTATTTACTGCCGGATCCTCAATTTAACCCACAAGACGGCGTTATCa                                                                                                                                      <  1:113103/180‑1 (MQ=255)                                                                                                                                                        attTGGATATTATGTGCCACTTTTTTAATTATACGGAACTGGAGTGATGTCGCAAATTTCGCATTGAGCGATACTGATGATCTTATGCGCTATCATCAATATAGTGAGTGGTTAAAAAATGGGAATTGGTATTTACTGCCGGATCCTCAATTTAACCCACAAGACGGCGTTATCATGCACTGGTCCAGAGTTGTGGATATTCCATTAGCCGGGGTAGCCCTGCTTGCTTCTATTTTCACCGATTGGAaca                                                           >  1:806832/1‑250 (MQ=255)                                                                                                                                                            ggATATTATGTGCCACTTTTTTAATTATACGGAACTGGAGTGATGTCGCAAATTTCGCATTGAGCGATACTGATGATCTTATGCGCTATCATCAATATAGTGAGTGGTTAAAAAATGGGAATTGGTATTTACTGCCGGATCCTCAATTTAACCCACAAGACGGCGTTATCATGCACTGGTCCAGAGTTGTGGATATTCCATTAGCCGGGGTAGCCCTGCTTGCTTCTATTTTCACCGATTGGAACACCGCa                                                      >  1:335323/1‑251 (MQ=255)                                                                                                                                                             gATATTATGTGCCACTTTTTTAATTATACGGAACTGGAGTGATGTCGCAAATTTCGCATTGAGCGATACTGATGATCTTATGCGCTATCATCAATATAGTGAGTGGTTAAAAAATGGGAATTGGTATTTACTGCCGGATCCTCAATTTAACCCACAAGACGGCGTTATCATGCACTGGTCCAGAGTTGTGGATATTCCATTAGCCGGGGTAGCCCTGCTTGCTTCt                                                                              <  1:209570/226‑1 (MQ=255)                                                                                                                                                                attatGTGCCACTTTTTTAATTATACGGAACTGGAGTGATGTCGCAAATTTCGCATTGAGCGATACTGATGATCTTATGCGCTATCATCAATATAGTGAGTGGTTAAAAAATGGGAATTGGTATTTACTGCCGGATCCTCAATTTAACCCACAAGACGGCGTTATCATGCACTGGTCCAGAGTTGTGGATATTCCATTAGCCGGGGTAGCCCTGCTTGCTTCTATTTTCACCGATTGGAACACCGCAt                                                     <  1:238992/248‑1 (MQ=255)                                                                                                                                                                 ttatGTGCCACTTTTTTAATTATACGGAACTGGAGTGATGTCGCAAATTTCGCATTGAGCGATACTGATGATCTTATGCGCTATCATCAATATAGTGAGTGGTTAAAAAATGGGAATTGGTATTTACTGCCGGATCCTCAATTTAACCCACAAGACGGCGTTATCATGCACTGGTCCAGAGTTGTGGATATTCCATTAGCCGGGGTAGCCCTGCTTGCTTCTATTTTCACCGATTGGAACACCGCATTCAc                                                 <  1:220898/251‑1 (MQ=255)                                                                                                                                                                                     tataCGGAACTGGAGTGATGTCGCAAATTTCGCATTGAGCGATACTGATGATCTTATGCGCTATCATCAATATAGTGAGTGGTTAAAAAATGGGAATTGGTATTTACTGCCGGATCCTCAATTTAACCCACAAGACGGCGTTATCATGCACTGGTCCAGAGTTGTGGATATTCCATTAGCCGGGGTAGCCCTGCTTGCTTCTATTTTCACCGATTGGAACACCGCATTCACCATATCGAATGTTATAacac                             <  1:690910/251‑1 (MQ=255)                                                                                                                                                                                           gAACTGGAGTGATGTCGCAAATTTCGCATTGAGCGATACTGATGATCTTATGCGCTATCATCAATATAGTGAGTGGTTAAAAAATGGGAATTGGTATTTACTGCCGGATCCTCAATTTAACCCACAAGACGGCGTTATCATGCACTGGTCCAGAGt                                                                                                                      >  1:617823/1‑156 (MQ=255)                                                                                                                                                                                           gAACTGGAGTGATGTCGCAAATTTCGCATTGAGCGATACTGATGATCTTATGCGCTATCATCAATATAGTGAGTGGTTAAAAAATGGGAATTGGTATTTACTGCCGGATCCTCAATTTAACCCACAAGACGGCGTTATCATGCACTGGTCCAGAGTTGTGGATATTCCATTAGCCGGGGTAGccc                                                                                         >  1:548977/1‑185 (MQ=255)                                                                                                                                                                                           gAACTGGAGTGATGTCGCAAATTTCGCATTGAGCGATACTGATGATCTTATGCGCTATCATCAATATAGTGAGTGGTTAAAAAATGGGAATTGGTATTTACTGCCGGATCCTCAATTTAACCCACAAGACGGCGTTATCATGCACTGGTCCAGAGTTGTGGATATTCCATTAGCCGGGGTAGCCCTGCTTGCTTCTATTTTCACCGATTGGAac                                                            <  1:118337/214‑1 (MQ=255)                                                                                                                                                                                           gAACTGGAGTGATGTCGCAAATTTCGCATTGAGCGATACTGATGATCTTATGCGCTATCATCAATATAGTGAGTGGTTAAAAAATGGGAATTGGTATTTACTGCCGGATCCTCAATTTAACCCACAAGACGGCGTTATCATGCACTGGTCCAGAGTTGTGGATATTCCATTAGCCGGGGTAGCCCTGCTTGCTTCTATTTTCACCGATTGGAACACCGCATTCAcc                                                <  1:446711/226‑1 (MQ=255)                                                                                                                                                                                           gAACTGGAGTGATGTCGCAAATTTCGCATTGAGCGATACTGATGATCTTATGCGCTATCATCAATATAGTGAGTGGTTAAAAAATGGGAATTGGTATTTACTGCCGGATCCTCAATTTAACCCACAAGACGGCGTTATCATGCACTGGTCCAGAGTTGTGGATATTCCATTAGCCGGGGTAGCCCTGCTTGCTTCTATTTTCACCGATTGGAACACCGCATTCACCATATCGAATGTTATAacac                             >  1:770010/1‑245 (MQ=255)                                                                                                                                                                                           gAACTGGAGTGATGTCGCAAATTTCGCATTGAGCGATACTGATGATCTTATGCGCTATCATCAATATAGTGAGTGGTTAAAAAATGGGAATTGGTATTTACTGCCGGATCCTCAATTTAACCCACAAGACGGCGTTATCATGCACTGGTCCAGAGTTGTGGATATTCCATTAGCCGGGGTAGCCCTGCTTGCTTCTATTTTCACCGATTGGAACACCGCATTCACCATATCGAATGTTATAACACCACTc                        >  1:376701/1‑250 (MQ=255)                                                                                                                                                                                             aCTGGAGTGATGTCGCAAATTTCGCATTGAGCGATACTGATGATCTTATGCGCTATCATCAATATAGTGAGTGGTTAAAAAATGGGAATTGGTATTTACTGCCGGATCCTCAATTTAACCCACAAGACGGCGTTATCATGCACTGGTCCAGAGTTGTGGATATTCCATTAGCCGGGGTAGCCCTGCTTGCTTCTATTTTCACCGATTGGAACACCGCATTCACCATATCGAATGTTATAACACCACTCCtt                     <  1:431868/251‑1 (MQ=255)                                                                                                                                                                                                 gAGTGATGTCGCAAATTTCGCATTGAGCGATACTGATGATCTTATGCGCTATCATCAATATAGTGAGTGGTTAAAAAATGGGAATTGGTATTTACTGCCGGATCCTCAATTTAACCCACAAGACGGCGTTATCATGCACTGGTCCAGAGTTGTGGATATTCCATTAGCCGGGGTAGCCCTGCTTGCTTCTATTTTCACCGATTGGAACACCGCATTCACCATATCGAATGTTATAACACCACTCCTTTAt                  >  1:659142/1‑250 (MQ=255)                                                                                                                                                                                                        gTCGCAAATTTCGCATTGAGCGATACTGATGATCTTATGCGCTATCATCAATATAGTGAGTGGTTAAAAAATGGGAATTGGTATTTACTGCCGGATCCTCAATTTAACCCACAAGACGGCGTTATCATGCACTGGTCCAGAGTTGTGGat                                                                                                               >  1:671963/1‑150 (MQ=255)                                                                                                                                                                                                                tttCGCATTGAGCGATACTGATGATCTTATGCGCTATCATCAATATAGTGAGTGGTTAAAAAATGGGAATTGGTATTTACTGCCGGATCCTCAATTTAACCCACAAGACGGCGTTATCATGCACTGGTCCAGAGTTGTGGATATTCCATTAGCCGGGGTAGCCCTGCTTGCTTCTATTTTCACCGATTGGAACACCGCATTCACCATATCGAATGTTATAACACCACTCCTTTATCTATTAATTTTTttat  <  1:308377/251‑1 (MQ=255)                                                                                                                                                                                                                            |                                                                                                                                                                                                                                               TTCCGGTATAAGCCTAATCCTAGGTTTTTTATATTCCAGATATATTGTTTTTCGAAATAATAAGTAATAATTAAACACTAAAAAGGTTAATATGTATAATAAATTATTTATAAATAATGACATAAAAAAATTAGTTTATATCGCTTGTATTATTTGGATATTATGTGCCACTTTTTTAATTATACGGAACTGGAGTGATGTCGCAAATTTCGCATTGAGTGATACTGATGATCTTATGCGCTATCATCAATATAGTGAGTGGTTAAAAAATGGGAATTGGTATTTACTGCCGGATCCTCAATTTAACCCACAAGACGGCGTTATCATGCACTGGTCCAGAGTTGTGGATATTCCATTAGCCGGGGTAGCCCTGCTTGCTTCTATTTTCACCGATTGGAACACCGCATTCACCATATCGAATGTTATAACACCACTCCTTTATCTATTAATTTTTTTAT  >  1/1647068‑1647525

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑