Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,647,186	G→A	intergenic (+5347/+17189)	unknown → / ← unknown	tRNA‑Gly‑GCC/LSU rRNA ## 23S rRNA, large subunit ribosomal RNA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,647,186	0	G	A	100.0%	20.6 / NA	10	intergenic (+5347/+17189)	unknown/unknown	tRNA‑Gly‑GCC/LSU rRNA ## 23S rRNA, large subunit ribosomal RNA
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (6/4);  total (6/4)

GAGTTATCTCATAGGATTGATAGCCGATAAAATAGAAGCGCTCCCGGCAATTACGCTAATAACCTCTTCCGGTATAAGCCTAATCCTAGGTTTTTTATATTCCAGATATATTGTTTTTCGAAATAATAAGTAATAATTAAACACTAAAAAGGTTAATATGTATAATAAATTATTTATAAATAATGACATAAAAAAATTAGTTTATATCGCTTGTATTATTTGGATATTATGTGCCACTTTTTTAATTATACGGAACTGGAGTGATGTCGCAAATTTCGCATTGAGTGATACTGATGATCTTATGCGCTATCATCAATATAGTGAGTGGTTAAAAAATGGGAATTGGTATTTACTGCCGGATCCTCAATTTAACCCACAAGACGGCGTTATCATGCACTGGT  >  1/1647002‑1647402                                                                                                                                                                                         |                                                                                                                                                                                                                         gAGTTATCTCATAGGATTGATAGCCGATAAAATAGAAGCGCTCCCGGCAATTACGCTAATAACCTCTTCCGGTATAAGCCTAATCCTAGGTTTTTTATATTCCAGATATATTGTTTTTCGAAATAATAAGTAATAATTAAACACTAAAAAGGTTAATATGTATAATAAATTATTTATAAATAATAACATAAAAAAATTAGTTTATATCGCTTGTATTATTTGGATATTATGTGCCACt                                                                                                                                                                     <  1:5869/238‑1 (MQ=255)      aTCTCATAGGATTGATAGCCGATAAAATAGAAGCGCTCCCGGCAATTACGCTAATAACCTCTTCCGGTATAAGCCTAATCCTAGGTTTTTTATATTCCAGATATATTGTTTTTCGAAATAATAAGTAATAATTAAACACTAAAAAGGTTAATATGTATAATAAATTATTTATAAATAATAACATAAAAAAATTAGTTTATATCGCTTGTATTATTTGGATATTATGTGCCACTTTTTTAATTATACGGaa                                                                                                                                                    >  1:763812/1‑250 (MQ=255)                                                                   ttCCGGTATAAGCCTAATCCTAGGTTTTTTATATTCCAGATATATTGTTTTTCGAAATAATAAGTAATAATTAAACACTAAAAAGGTTAATATGTATAATAAATTATTTATAAATAATAACATAAAAAAATTAGTTTATATCGCTTGTATTATTTGGATATTATGTGCCACTTTTTTAATTATACGGAACTGGAGTGATGTCGCAAATTTCGCATTGAGCGATACTGATGATCTTATGCGCTATCATCAat                                                                                      <  1:842868/251‑1 (MQ=255)                                                                                               ttATATTCCAGATATATTGTTTTTCGAAATAATAAGTAATAATTAAACACTAAAAAGGTTAATATGTATAATAAATTATTTATAAATAATAACATAAAAAAATTAGTTTATATCGCTTGTATTATTTGGATATTATGTGCCACTTTTTTAATTATACGGAACTGGAGTGATGTCGCAAATTTCGCATTGAGCGATACTGATGATCTTATGCGCTATCATCAATATAGTGAGTGGTTaaaaaa                                                                   >  1:560144/1‑242 (MQ=255)                                                                                                                 gTTTTTCGAAATAATAAGTAATAATTAAACACTAAAAAGGTTAATATGTATAATAAATTATTTATAAATAATAACATAAAAAAATTAGTTTATATCGCTTGTATTATTTGGATATTATGTGCCACTTTTTTAATTATACGGAACTGGAGTGATGTCGCAAATTTCGCATTGAGCGATACTGATGATCTTATGCGCTATCATCAATATAGTGAGTGGTTAAAAAATGGGAATTGGTATTTACTGCCGGATc                                         >  1:480259/1‑250 (MQ=255)                                                                                                                             aataaGTAATAATTAAACACTAAAAAGGTTAATATGTATAATAAATTATTTATAAATAATAACATAAAAAAATTAGTTTATATCGCTTGTATTATTTGGATATTATGTGCCACTTTTTTAATTATACGGAACTGGAGTGATGTCGCAAATTTCGCATTGAGCGATACTGATGATCTTATGCGCTATCATCAATATAGTGAGTGGTTAAAAAATGGGAATTGGTATTTACTGCCGGATCCTCAATTTAAcc                             >  1:291422/1‑250 (MQ=255)                                                                                                                                  gTAATAATTAAACACTAAAAAGGTTAATATGTATAATAAATTATTTATAAATAATAACATAAAAAAATTAGTTTATATCGCTTGTATTATTTGGATATTATGTGCCACTTTTTTAAt                                                                                                                                                             >  1:58311/1‑117 (MQ=255)                                                                                                                                                   aaaaGGTTAATATGTATAATAAATTATTTATAAATAATAACATAAAAAAATTAGTTTATATCGCTTGTATTATTTGGATATTATGTGCCACTTTTTTAATTATACGGAACTGGAGTGATGTCGCAAATTTCGCATTGAGCGATACTGATGATCTTATGCGCTATCATCAATATAGTGAGTGGTTAAAAAATGGGAATTGGTATTTACTGCCGGATCCTCAATTTAACCCACAAGACGGCGTTATCATGCAc      <  1:252364/251‑1 (MQ=255)                                                                                                                                                        gTTAATATGTATAATAAATTATTTATAAATAATAACATAAAAAAATTAGTTTATATCGCTTGTATTATTTGGATATTATGTGCCACTTTTTTAATTATACGGAACTGGAGTGATGTCGCAAATTTCGCATTGAGCGATACTGATGATCTTATGCGCTATCATCAATATAGTGAGTGGTTAAAAAATGGGAATTGGTATTTACTGCCGGATCCTCAATTTAACCCACAAGACGGCGTTATCATGCACTGGt  >  1:81424/1‑250 (MQ=255)                                                                                                                                                              atGTATAATAAATTATTTATAAATAATAACATAAAAAAATTAGTTTATATCGCTTGTATTATTTGGATATTATGTGCCACTTTTTTAATTATACGGAACTGGAGTGATGTCGCAAATTTCGCATTGAGCGATACTGATGATCTTATGCGCTATCATCAATATAGTGAGTGGTTAAAAAATGGGAATTGGTATTTACTGCCGGATCCTCAATTTAACCCACAAGACGGCGTTATCATGCaa      <  1:538508/240‑2 (MQ=255)                                                                                                                                                                                         |                                                                                                                                                                                                                         GAGTTATCTCATAGGATTGATAGCCGATAAAATAGAAGCGCTCCCGGCAATTACGCTAATAACCTCTTCCGGTATAAGCCTAATCCTAGGTTTTTTATATTCCAGATATATTGTTTTTCGAAATAATAAGTAATAATTAAACACTAAAAAGGTTAATATGTATAATAAATTATTTATAAATAATGACATAAAAAAATTAGTTTATATCGCTTGTATTATTTGGATATTATGTGCCACTTTTTTAATTATACGGAACTGGAGTGATGTCGCAAATTTCGCATTGAGTGATACTGATGATCTTATGCGCTATCATCAATATAGTGAGTGGTTAAAAAATGGGAATTGGTATTTACTGCCGGATCCTCAATTTAACCCACAAGACGGCGTTATCATGCACTGGT  >  1/1647002‑1647402

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑