Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,646,793	C→A	intergenic (+4954/+17582)	unknown → / ← unknown	tRNA‑Gly‑GCC/LSU rRNA ## 23S rRNA, large subunit ribosomal RNA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,646,793	0	C	A	100.0%	26.4 / NA	12	intergenic (+4954/+17582)	unknown/unknown	tRNA‑Gly‑GCC/LSU rRNA ## 23S rRNA, large subunit ribosomal RNA
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (7/5);  total (7/5)

ATTTGCACACTATTTTCACTGATTACCGGTACTTCCGTTATCTTCCAGTATCTCAAGCTTGGTACGGTACTAAAAATATCTACTGCTGTATTCGCTACCGGCATGGGGATTCTGGCTATATTATTTTTTGCAATTGCATTAATTCTAGACTCAATATCCAATGCAAGAAAAGAACTTAAAAGAATGAATTATCTCAATATTAAATAAAAAATGATAAAGCAATTCTTTCGATACTTCTCAGTAGGTATCATAAATACAATAATACACTGGGGAGTATTTTATATTTTCTATCGGTTAACATACGTACAGTCATTAAGCAACCTTATAGGATTCAGTACCGCCGTAATATTCTCTTTTTTTGTAAATTCAAAATATAC  >  1/1646565‑1646941                                                                                                                                                                                                                                     |                                                                                                                                                     aTTTGCACACTATTTTCACTGATTACCGGTACTTCCGTTATCTTCCAGTATCTCAAGCTTGGTACGGTACTAAAAATATCTACTGCTGTATTCGCTACCGGCATGGGGATTCTGGCTATATTATTTTTTGCAATTGCATTAATTCTAGACTCAATATCCAATGCAAGAAAAGAACTTAAAAGAATGAATTATCTCAATATTAAATAAAAAATGATAAAGCAATTCTTTAGATACTTCTCAGt                                                                                                                                         <  1:637523/242‑1 (MQ=255)          cTATTTTCACTGATTACCGGTACTTCCGTTATCTTCCAGTATCTCAAGCTTGGTACGGTACTAAAAATATCTACTGCTGTATTCGCTACCGGCATGGGGATTCTGGCTATATTATTTTTTGCAATTGCATTAATTCTAGACTCAATATCCAATGCAAGAAAAGAACTTAAAAGAATGAATTATCTCAATATTAAATAAAAAATGATAAAGCAATTCTTTAGATACTTCTCAGt                                                                                                                                         >  1:49835/1‑233 (MQ=255)          cTATTTTCACTGATTACCGGTACTTCCGTTATCTTCCAGTATCTCAAGCTTGGTACGGTACTAAAAATATCTACTGCTGTATTCGCTACCGGCATGGGGATTCTGGCTATATTATTTTTTGCAATTGCATTAATTCTAGACTCAATATCCAATGCAAGAAAAGAACTTAAAAGAATGAATTATCTCAATATTAAATAAAAAATGATAAAGCAATTCTTTAGATACTTCTCAGTAGGTATCATAAATAc                                                                                                                          <  1:687251/248‑1 (MQ=255)                 cACTGATTACCGGTACTTCCGTTATCTTCCAGTATCTCAAGCTTGGTACGGTACTAAAAATATCTACTGCTGTATTCGCTACCGGCATGGGGATTCTGGCTATATTATTTTTTGCAATTGCATTAATTCTAGACTCAATATCCAATGCAAGAAAAGAACTTAAAAGAATGAATTATCTCAATATTAAATAAAAAATGATAAAGCAATTCTTTAGATACTTCTCAGTAGGTATCATAAATACAATAATacac                                                                                                                <  1:163927/251‑1 (MQ=255)                                             ccAGTATCTCAAGCTTGGTACGGTACTAAAAATATCTACTGCTGTATTCGCTACCGGCATGGGGATTCTGGCTATATTATTTTTTGCAATTGCATTAATTCTAGACTCAATATCCAATGCAAGAAAAGAACTTAAAAGAATGAATTATCTCAATATTAAATAAAAAATGATAAAGCAATTCTTTAGATACTTCTCAGt                                                                                                                                         >  1:63276/1‑198 (MQ=255)                                                                                   ctgctgTATTCGCTACCGGCATGGGGATTCTGGCTATATTATTTTTTGCAATTGCATTAATTCTAGACTCAATATCCAATGCAAGAAAAGAACTTAAAAGAATGAATTATCTCAATATTAAATAAAAAATGATAAAGCAATTCTTTAGATACTTCTCAGTAGGTa                                                                                                                                    >  1:133546/1‑165 (MQ=255)                                                                                                             aTTCTGGCTATATTATTTTTTGCAATTGCATTAATTCTAGACTCAATATCCAATGCAAGAAAAGAACTTAAAAGAATGAATTATCTCAATATTAAATAAAAAATGATAAAGCAATTCTTTAGATACTTCTCAGTAGGTATCATAAATACAATAATACACTGGGGAGTATTTTATATTTTCTATCGGTTAACATGCGTACAGTCATTAAGCAACCTTATAGGAt                                                <  1:291635/223‑1 (MQ=255)                                                                                                                  ggCTATATTATTTTTTGCAATTGCATTAATTCTAGACTCAATATCCAATGCAAGAAAAGAACTTAAAAGAATGAATTATCTCAATATTAAATAAAAAATGATAAAGCAATTCTTTAGATACTTCTCAGTAg                                                                                                                                       >  1:491827/1‑131 (MQ=255)                                                                                                                               tttGCAATTGCATTAATTCTAGACTCAATATCCAATGAAAGAAAAGAACTTAAAAGAATGAATTATCTAAATATTAAAAAAAAAATGAAAAAGCAATTCTTTAGATACTTCTCAGTAGGTATCATAAATAAAATAATACACTGGGGAGTATTTTATATTTTCTATCGGTTAACATACGTACAGTCATTAAGAAACCTTATAGGATTCAGTACCGCCGTAATATTCTCTTTTTTTATAAATTCAAAATATAc  <  1:380282/251‑1 (MQ=255)                                                                                                                                                        cAATATCCAATGCAAGAAAAGAACTTAAAAGAATGAATTATCTCAATATTAAATAAAAAATGATAAAGCAATTCTTTAGATACTTCTCAGTAGGTATCATAAATACAATAATACACTGGGGAGTAttt                                                                                                    >  1:272785/1‑128 (MQ=255)                                                                                                                                                                                                          aaataaaAAATGATAAAGCAATTCTTTAGATACTTCTCAGTAg                                                                                                                                       >  1:224773/1‑43 (MQ=255)                                                                                                                                                                                                                                tCTTTAGATACTTCTCAGTAGGTATCATAAATACAATAATACACTGGGGAGTATTTTATATTTTCTATCGGTTAACATACGTACAGTCATTAAGCAACCTTATAg                                                   >  1:881592/1‑105 (MQ=255)                                                                                                                                                                                                                                     |                                                                                                                                                     ATTTGCACACTATTTTCACTGATTACCGGTACTTCCGTTATCTTCCAGTATCTCAAGCTTGGTACGGTACTAAAAATATCTACTGCTGTATTCGCTACCGGCATGGGGATTCTGGCTATATTATTTTTTGCAATTGCATTAATTCTAGACTCAATATCCAATGCAAGAAAAGAACTTAAAAGAATGAATTATCTCAATATTAAATAAAAAATGATAAAGCAATTCTTTCGATACTTCTCAGTAGGTATCATAAATACAATAATACACTGGGGAGTATTTTATATTTTCTATCGGTTAACATACGTACAGTCATTAAGCAACCTTATAGGATTCAGTACCGCCGTAATATTCTCTTTTTTTGTAAATTCAAAATATAC  >  1/1646565‑1646941

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑