Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,636,132	A→G	intergenic (‑191504/‑5632)	unknown ← / → unknown	tRNA‑Met‑CAT/tRNA‑Gly‑GCC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,636,132	0	A	G	100.0%	163.7 / NA	60	intergenic (‑191504/‑5632)	unknown/unknown	tRNA‑Met‑CAT/tRNA‑Gly‑GCC
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (34/26);  total (34/26)

GAATTACAGGAATTAAAAGATTTCTGCAAAACACACTATAATTTGACCGCACTTGAGCTTTGGGATTTGTCCTTTTACAGCGAGAAACAAAAACAACATTTATATGCCATTAGCGATGAAGAATTGCGCCCTTATTTCCCGGAAGATCGTGTCCTTAACGGCTTATTTGAACTGATTAAACGCATTTTCAACATTCGTGCCGTAGAACGTCATGATGTGGAAACCTGGCATAAAGATGTGCACTTCTTTGACTTAATTGATGAAAATGATGAAGTTCGCGGCAGCTTCTATTTAGATTTGTATGCCCGTGAACATAAACGCGGCGGCGCATGGATGGACGATTGTATCGGTCGTCGCAAAACCATCGACGGTAGTCTACAAAAACCGGTCGCCTATTTAACCTGTAACTTCAATCGTCCGCTCGGTGATCAACCGGCATTATTCACCCATGATGAAGTCACTACCCTGTTCCATGAAT  >  1/1635891‑1636368                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                             gAATTACAGGAATTAAAAGATTTCTGCAAAACACACTATAATTTGACCGCACTTGAGCTTTGGGATTTGTCCTTTTACAGCGAGAAACAAAAACAACATTTATATGCCATTAGCGATGAAGAATTGCGCCCTTATTTCCCGGAAGATCGTGTCCTTAACGGCTTATTTGCACTGATTAAACGCATTTTCAACATTCGTGCCGTAGAACGTCATGATGTGGAAACCTGGCATAAAGATGTGCGCTTCTTTGa                                                                                                                                                                                                                                     >  1:434373/1‑251 (MQ=255)  aaTTACAGGAATTAAAAGATTTCTGCAAAACACACTATAATTTGACCGCACTTGAGCTTTGGGATTTGTCCTTTTACAGCGAGAAACAAAAACAACATTTATATGCCATTAGCGATGAAGAATTGCGCCCTTATTTCCCGGAAGATCGTGTCCTTAACGGCTTATTTGAACTGATTAAACGCATTTTCAACATTCGTGCCGTAGAACGTCATGATGTGGAAACCTGGCATAAAGATGTGCGCTTCTTTGa                                                                                                                                                                                                                                     >  1:312525/1‑250 (MQ=255)                             aaaCACACTATAATTTGACCGCACTTGAGCTTTGGGATTTGTCCTTTTACAGCGAGAAACAAAAACAACATTTATATGCCATTAGCGATGAAGAATTGCGCCCTTATTTCCCGGAAGATCGTGTCCTTAACGGCTTATTTGAACTGATTAAACGCATTTTCAACATTCGTGCCGTAGAACGTCATGATGTGGAAACCTGGCATAAAGATGTGCGCTTCTTTGACTTAATTGATGAAAATGATGAAGTTcg                                                                                                                                                                                                          >  1:250915/1‑250 (MQ=255)                                  cacTATAATTTGACCGCACTTGAGCTTTGGGATTTGTCCTTTTACAGCGAGAAACAAAAACAACATTTATATGCCATTAGCGATGAAGAATTGCGCCCTTATTTCCCGGAAGATCGTGTCCTTAACGGCTTATTTGAACTGATTAAACGCATTTTCAACATTCGTGCCGTAGAACGTCATGATGTGGAAACCTGGCATAAAGATGTGCGCTTCTTTGACTTAATTGATGAAAATGATGAAGTTCGCGGCAg                                                                                                                                                                                                    >  1:395977/1‑251 (MQ=255)                                  cacTATAATTTGACCGCACTTGAGCTTTGGGATTTGTCCTTTTACAGCGAGAAACAAAAACAACATTTATATGCCATTAGCGATGAAGAATTGCGCCCTTATTTCCCGGAAGATCGTGTCCTTAACGGCTTATTTGAACTGATTAAACGCATTTTCAACATTCGTGCCGTAGAACGTCATGATGTGGAAACCTGGCATAAAGATGTGCGCTTCTTTGACTTAATTGATGAAAATGATGAAGTTCGCGGCAg                                                                                                                                                                                                    >  1:220033/1‑251 (MQ=255)                                  cacTATAATTTGACCGCACTTGAGCTTTGGGATTTGTCCTTTTACAGCGAGAAACAAAAACAACATTTATATGCCATTAGCGATGAAGAATTGCGCCCTTATTTCCCGGAAGATCGTGTCCTTAACGGCTTATTTGAACTGATTAAACGCATTTTCAACATTCGTGCCGTAGAACGTCATGATGTGGAAACCTGGCATAAAGATGTGCGCTTCTTTGACTTAATTGATGAAAATGATGAAGTTCGCGGCAg                                                                                                                                                                                                    >  1:805726/1‑251 (MQ=255)                                  cacTATAATTTGACCGCACTTGAGCTTTGGGATTTGTCCTTTTACAGCGAGAAACAAAAACAACATTTATATGCCATTAGCGATGAAGAATTGCGCCCTTATTTCCCGGAAGATCGTGTCCTTAACGGCTTATTTGAACTGATTAAACGCATTTTCAACATTCGTGCCGTAGAACGTCATGATGTGGAAACCTGGCATAAAGATGTGCGCTTCTTTGACTTAATTGATGAAAATGATGAAGTTCGCGGCAg                                                                                                                                                                                                    >  1:794646/1‑251 (MQ=255)                                    cTATAATTTGACCGCACTTGAGCTTTGGGATTTGTCCTTTTACAGCGAGAAACAAAAACAACATTTATATGCCATTAGCGATGAAGAATTGCGCCCTTATTTCCCGGAAGATCGTGTCCTTAACGGCTTATTTGAACTGATTAAACGCATTTTCAACATTCGTGCCGTAGAACGTCATGATGTGGAAACCTGGCATAAAGATGTGCGCTTCTTTGACTTAATTGATGAAAATGATGAAGTTCGcagcag                                                                                                                                                                                                    >  1:493236/1‑249 (MQ=255)                                                        aGCTTTGGGATTTGTCCTTTTACAGCGAGAAACAAAAACAACATTTATATGCCATTAGCGATGAAGAATTGCGCCCTTATTTCCCGGAAGATCGTGTCCTTAACGGCTTATTTGAACTGATTAAACGCATTTTCAACATTCGTGCCGTAGAACGTCATGATGTGGAAACCTGGCATAAAGATGTGCGCTTCTTTGACTTAATTGATGAAAATGATGAAGTTCGCGGCAGCTTCTATTTAGATTTGTATGc                                                                                                                                                                               >  1:82425/1‑250 (MQ=255)                                                              gggATTTGTCCTTTTACAGCGAGAAACAAAAACAACATTTATATGCCATTAGCGATGAAGAATTGCGCCCTTATTTCCCGGAAGATCGTGTCCTTAACGGCTTATTTGAACTGATTAAACGCATTTTCAACATTCGTGCCGTAGAACGTCATGATGTGGAAACCTGGCATAAAGATGTGCGCTTCTTTGACTTAATTGATGAAAATGATGAAGTTCGCGGCAGCTTCTATTTAGATTTGTATGCCCGTGaa                                                                                                                                                                        >  1:746442/1‑251 (MQ=255)                                                                     gTCCTTTTACAGCGAGAAACAAAAACAACATTTATATGCCATTAGCGATGAAGAATTGCGCCCTTATTTCCCGGAAGATCGTGTCCTTAACGGCTTATTTGAACTGATTAAACGCATTTTCAACATTCGTGCCGTAGAACGTCATGATGTGGAAACCTGGCATAAAGATGTGCGCTTCTTTGACTTAATTGATGAAAATGATGAAGTTCGCGGCAGCTTCTATTTAGATTTGTATGCCCGTGAACATaaa                                                                                                                                                                  >  1:472376/1‑250 (MQ=255)                                                                     gTCCTTTTACAGCGAGAAACAAAAACAACATTTATATGCCATTAGCGATGAAGAATTGCGCCCTTATTGCCCGGAAGATCGTGTCCTTAACGGCTTATTTGAACTGATTAAACGCATTTTCAACATTCGTGCCGTAGAACGTCATGATGTGGAAACCTGGCATAAAGATGTGCGCTTCTTTGACTTAATTGATGAAAATGATGAAGTTCGCGGCAGCTTCTATTTAGATTTGTATGcc                                                                                                                                                                              <  1:88152/238‑1 (MQ=255)                                                                        cTTTTACAGCGAGAAACAAAAACAACATTTATATGCCATTAGCGATGAAGAATTGCGCCCTTATTTCCCGGAAGATCGTGTCCTTAACGGCTTATTTGAACTGATTAAACGCATTTTCAACATTCGTGCCGTAGAACGTCATGATGTGGAAACCTGGCATAAAGATGTGCGCTTCTTTGACt                                                                                                                                                                                                                                   >  1:28228/1‑182 (MQ=255)                                                                                     aaacaaaaacaaCATTTATATGCCATTAGCGATGAAGAATTGCGCCCTTATTTCCCGGAAGATCGTGTCCTTAACGGCTTATTTGAACTGATTAAACGCATTTTCAACATTCGTGCCGTAGAACGTCATGATGTGGAAGCCTGGCATAAAGATGTGCGCTTCTTTGACTTAATTGATGAAAATGATGAAGTTCGCGGCAGCTTCTATTTAGATTTGTATGCCCGTGAACATAAACGCGGCGGCGCatggat                                                                                                                                                 >  1:368836/1‑251 (MQ=255)                                                                                       acaaaaacaaCATTTATATGCCATTAGCGATGAAGAATTGCGCCCTTATTTCCCGGAAGATCGTGTCCTTAACGGCTTATTTGAACTGATTAAACGCATTTTCAACATTCGTGCCGTAGAACGTCATGATGTGGAAACCTGGCATAAAGATGTGCGCTTCTTTGACTTAATTGATGAAAATGATGAAGTTCGCGGCAGCTTCTATTTAGATTTGTATGCCCGTGAACATAAACGCGGCGGCGCatggatgg                                                                                                                                               >  1:416290/1‑251 (MQ=255)                                                                                         aaaaacaaCATTTATATGCCATTAGCGATGAAGAATTGCGCCCTTATTTCCCGGAAGATCGTGTCCTTAACGGCTTATTTGAACTGATTAAACGCATTTTCAACATTCGTGCCGTAGAACGTCATGATGTGGAAACCTGGCATAAAGATGTGCGCTTCTTTGACTTAATTGATGAAAATGATGAAGTTCGCGGCAGCTTCTATTTAGATTTGTATGCCCGTGAACATAAACGCGGCGGCGCATGGATGGAc                                                                                                                                             <  1:108808/251‑1 (MQ=255)                                                                                           aaacaaCATTTATATGCCATTAGCGATGAAGAATTGCGCCCTTATTTCCCGGAAGATCGTGTCCTTAACGGCTTATTTGAACTGATTAAACGCATTTTCAACATTCGTGCCGTAGAACGTCATGATGTGGAAACCTGGCATAAAGATGTGCGCTTCTTTGACTTAATTGATGAAAATGATGAAGTTCGCGGCAGCTTCTATTTAGATTTGTATGCCCGTGAACATAAACGCGGCGGCGCATGGATGGACGa                                                                                                                                           >  1:419075/1‑251 (MQ=255)                                                                                            aacaacATTTATATGCCATTAGCGATGAAGAATTGCGCCCTTATTTCCCGGAAGATCGTGTCCTTAACGGCTTATTTGAACTGATTAAACGCATTTTCAACATTCGTGCCGTAGAACGTCATGATGTGGAAACCTGGCATAAAGATGTGCGCTTCTTTGACTTAATTGATGAAAATGATGAAGTTCGCGGCAGCTTCTATTTAGATTTGTATGCCCGTGAACATAAACGCGGCGGCGCATGGATGGACGAt                                                                                                                                          <  1:848699/251‑1 (MQ=255)                                                                                                acaTTTATATGCCATTAGCGATGAAGAATTGCGCCCTTATTTCCCGGAAGATCGTGTCCTTAACGGCTTATTTGAACTGATTAAACGCATTTTCAACATTCGTGCCGTAGAACGTCATGATGTGGAAACCTGGCATAAAGATGTGCGCTTCTTTGACTTAATTGATGAAAATGATGAAGTTCGCGGCAGCTTCTATTTAGATTTGTATGCCCGTGAACATAAACGCGGCGGCGCATGGATGGACGATTGTa                                                                                                                                      >  1:60854/1‑251 (MQ=255)                                                                                                 caTTTATATGCCATTAGCGATGAAGAATTGCGCCCTTATTTCCCGGAAGATCGTGTCCTTAACGGCTTATTTGAACTGATTAAACGCATTTTCAACACTCGTGCCGTAGAACGTCATGATGTGGAAACCTGGCATAAAGATGTGCGCTTCTTTGACTTAATTGATGAAAATGATGAAGTTCGCGGCAGCTTCTATTTAGAtt                                                                                                                                                                                      <  1:146067/202‑1 (MQ=255)                                                                                                   tttATATGCCATTAGCGATGAAGAATTGCGCCCTTATTTCCCGGAAGATCGTGTCCTTAACGGCTTATTTGAACTGATTAAACGCATTTTCAACATTCGTGCCGTAGAACGTCATGATGTGGAAACCTGGCATAAAGATGTGCGCTTCTTTGACTTAATTGATGAAAATGATGAAGTTCGCGGCAGCTTCTATTTAGATTTGTATGCCCGTGAACATAAACGCGGCGGCGCATGGATGGACGATTGTATCg                                                                                                                                   >  1:841852/1‑251 (MQ=255)                                                                                                           ccATTAGCGATGAAGAATTGCGCCCTTATTTCCCGGAAGATCGTGTCCTTAACGGCTTATTTGAACTGATTAAACGCATTTTCAACATTCGTGCCGTAGAACGTCATGATGTGGAAACCTGGCATAAAGATGTGCGCTTCTTTGACTTAATTGATGAAAATGATGAAGTTCGCGGCAGCTTCTATTTAGATTTGTATGCCCGTGAACATAAACGCGGCGGCGCATGGATGGACGAt                                                                                                                                          <  1:52550/236‑1 (MQ=255)                                                                                                            cATTAGCGATGAAGAATTGCGCCCTTATTTCCCGGAAGATCGTGTCCTTAACGGCTTATTTGAACTGATTAAACGCATTTTCAACATTCGTGCCGTAGAACGTCATGATGTGGAAACCTGGCATAAAGATGTGCGCTTCTTTGACTTAATTGATGAAAATGATGAAGTTCGCGGCAGCTTCTATTTAGATTTGTATGCCCGTGAACATAAACGCGGCGGCGCATGGATGGACGATTGTATCGgtc                                                                                                                                <  1:591656/245‑1 (MQ=255)                                                                                                                      gaagaaTTGCGCCCTTATTTCCCGGAAGATCGTGTCCTTAACGGCTTATTTGAACTGATTAAACGCATTTTCAACATTCGTGCCGTAGAACGTCATGATGTGGAAACCTGGCATAAAGATGTGCGCTTCTTTGACtt                                                                                                                                                                                                                                  >  1:264766/1‑137 (MQ=255)                                                                                                                      gaagaaTTGCGCCCTTATTTCCCGGAAGATCGTGTCCTTAACGGCTTATTTGAACTGATTAAACGCATTTTCAACATTCGTGCCGTAGAACGTCATGATGTGGAAACCTGGCATAAAGATGTGCGCTTCTTTGACTTAATTGATGAAAATGATGAAGTTCGCGGCAGCTTCTATTTAGAtt                                                                                                                                                                                      >  1:114130/1‑181 (MQ=255)                                                                                                                      gaagaaTTGCGCCCTTATTTCCCGGAAGATCGTGTCCTTAACGGCTTATTTGAACTGATTAAACGCATTTTCAACATTCGTGCCGTAGAACGTCATGATGTGGAAACCTGGCATAAAGATGTGCGCTTCTTTGACTTAATTGATGAAAATGATGAAGTTCGCGGCAGCTTCTATTTAGATTTGTATGCCCGTGAACATAAACGCGGCGGCGCATGGATGGACGATTGTATCGGTCGTCGCAAAACCATCGa                                                                                                                >  1:150491/1‑251 (MQ=255)                                                                                                                          aaTTGCGCCCTTATTTCCCGGAAGATCGTGTCCTTAACGGCTTATTTGAACTGATTAAACGCATTTTCAACATTCGTGCCGTAGAACGTCATGATGTGGAAACCTGGCATAAAGATGTGCGCTTCTTTGACTTAATTGATGAAAATGATGAAGTTCGCGGCAGCTTCTATTTAGATTTGTATGCCCGTGAACATAAACGCGGCGGCGCATGGATGGACGATTGTATCGGTCGTCGCAAAACCATCGACGGt                                                                                                            <  1:659145/251‑1 (MQ=255)                                                                                                                                   cTTATTTCCCGGAAGATCGTGTCCTTAACGGCTTATTTGAACTGATTAAACGCATTTTCAACATTCGTGCCGTAGAACGTCATGATGTGGAAACCTGGCATAAAGATGTGCGCTTCTTTGACTTAATTGATGAAAATGATGAAGTTCGCGGCAGCTTCTATTTAGATTTGTATGCCCGTGAACATAAACGCGGCGGCGCATGGATGGACGATTGTATCGGTCGTCGCAAAACCATCGACGGTAGTCTACaa                                                                                                   <  1:582881/251‑1 (MQ=255)                                                                                                                                   cTTATTTCCCGGAAGATCGTGTCCTTAACGGCTTATTTGAACTGATTAAACGCATTTTCAACATTCGTGCCGTAGAACGTCATGATGTGGAAACCTGGCATAAAGATGTGCGCTTCTTTGACTTAATTGATGAAAATGATGAAGTTCGCGGCAGCTTCTATTTAGATTTATATGCCCGTGAACATAAACGCGGCGGCGCATGGATGGACGATTGTATCGGTCGTCGCAAAACCATCGACGGTAGTCTACaa                                                                                                   >  1:448039/1‑251 (MQ=255)                                                                                                                                          cccGGAAGATCGTGTCCTTAACGGCTTATTTGAACTGATTAAACGCATTTTCAACATTCGTGCCGTAGAACGTCATGATGTGGAAACCTGGCATAAAGATGTGCGCTTCTTTGACTTAATTGATGAAAATGATGAAGTTCGCGGCAGCTTCTATTTAGATTTGTATGCCCGTGAACATAAACGCGGCGGCGCATGGATGGACGATTGTATCGGTCGTCGCAAAACCATCGACGGTAGTCTACAAAAAc                                                                                               <  1:719311/248‑1 (MQ=255)                                                                                                                                               aaGATCGTGTCCTTAACGGCTTATTTGAACTGATTAAACGCATTTTCAACATTCGTGCCGTAGAACGTCATGATGTGGAAACCTGGCATAAAGATGTGCGCTTCTTTGACTTAATTGATGAAAATGATGAAGTTCGCGGCAGCTTCTATTTAGAtt                                                                                                                                                                                      >  1:784477/1‑156 (MQ=255)                                                                                                                                                  aTCGTGTCCTTAACGGCTTATTTGAACTGATTAAACGCATTTTCAACATTCGTGCCGTAGAACGTCATGATGTGGAAACCTGGCATAAAGATGTGCGCTTCTTTGACTTAATTGATGAAAATGATGAAGTTCGCGGCAGCTTCTATTTAGATTTGTATGCCCGTGAACAt                                                                                                                                                                     >  1:242502/1‑170 (MQ=255)                                                                                                                                                        tCCTTAACGGCTTATTTGAACTGATTAAACGCATTTTCAACATTCGTGCCGTAGAACGTCATGATGTGGAAACCTGGCATAAAGATGTGCGCTTCTTTGACTTAATTGATGAAAATGATGAAGTTCGCGGCAGCTTCTATTTAGATTTGTATGCCCGTGAACATAAACGCGGCGGCGCATGGATGGACGATTGTATCGGTCGTCGCAAAACCATCGACGGTAGTCTACAAAAACCGGTCGCCTATTTAAcc                                                                              <  1:94792/251‑1 (MQ=255)                                                                                                                                                        tCCTTAACGGCTTATTTGAACTGATTAAACGCATTTTCAACATTCGTGCCGTAGAACGTCATGATGTGGAAACCTGGCATAAAGATGTGCGCTTCTTTGACTTAATTGATGAAAATGATGAAGTTCGCGGCAGCTTCTATTTAGATTTGTATGCCCGTGAACATAAACGCGGCGGCGCATGGATGGACGATTGTATCGGTCGTCGCAAAACCATCGACGGTAGTCTACAAAAACCGGTCGCCTATTTAAcc                                                                              <  1:727509/251‑1 (MQ=255)                                                                                                                                                        tCCTTAACGGCTTATTTGAACTGATTAAACGCATTTTCAACATTCGTGCCGTAGAACGTCATGATGTGGAAACCTGGCATAAAGATGTGCGCTTCTTTGACTTAATTGATGAAAATGATGAAGTTCGCGGCAGCTTCTATTTAGATTTGTATGCCCGTGAACATAAACGCGGCGGCGCATGGATGGACGATTGTATCGGTCGTCGCAAAACCATCGACGGTAGTCTACAAAAACCGGTCGCCTATTTAAc                                                                               <  1:362822/250‑1 (MQ=255)                                                                                                                                                             aaCGGCTTATTTGAACTGATTAAACGCATTTTCAACATTCGTGCCGTAGAACGTCATGATGTGGAAACCTGGCATAAAGATGTGCGCTTCTTTGACTTAATTGATGAAAATGATGAAGTTCGCGGCAGCTTCTATTTAGATTTGTATGCCCGTGAACATAAACGCGGCGGCGCATGGATGGACGATTGTATCGGTCGTCGCAAAACCATCGACGGTAGTCTACAAAAACCGGTCGCCTATTTAACCTGTaa                                                                         <  1:534925/251‑1 (MQ=255)                                                                                                                                                                    tATTTGAACTGATTAAACGCATTTTCAACATTCGTGCCGTAGAACGTCATGATGTGGAAACCTGGCATAAAGATGTGCGCTTCTTTGACTTAATTGATGAAAATGATGAAGTTCGCGGCAGCTTCTATTTAGAtt                                                                                                                                                                                      <  1:694555/135‑1 (MQ=255)                                                                                                                                                                              gATTAAACGCATTTTCAACATTCGTGCCGTAGAACGTCATGATGTGGAAACCTGGCATAAAGATGTGCGCTTCTTTGACTTAATTGATGAAAATGATGAAGTTCGCGGCAGCTTCTATTTAGAtt                                                                                                                                                                                      >  1:581727/1‑125 (MQ=255)                                                                                                                                                                              gATTAAACGCATTTTCAACATTCGTGCCGTAGAACGTCATGATGTGGAAACCTGGCATAAAGATGTGCGCTTCTTTGACTTAATTGATGAAAATGATGAAGTTCGCGGCAGCTTCTATTTAGATTTGTATGCCCGTGAACATAAACGCGGCGGCGCATGGATGGACGATTGTATCGGTCGTCGCAAAACCATCGACGGTAGTCTACAAAAACCGGTCGCCTATTTAACCTGTAACTTCAATCGTCCGCTCg                                                        >  1:245160/1‑251 (MQ=255)                                                                                                                                                                                       cATTTTCAACATTCGTGCCGTAGAACGTCATGATGTGGAAACCTGGCATAAAGATGTGCGCTTCTTTGACTTAATTGATGAAAATGATGAAGTTCGCGGCAGCTTCTATTTAGATTTGTATGCCCGTGAACATAAACGCGGCGGCGCATGGATGGACGATTGTATCGGTCGTCGCAAAACCATCGACGGTAGTCTACAAAAAc                                                                                               >  1:40345/1‑203 (MQ=255)                                                                                                                                                                                           ttCAACATTCGTGCCGTAGAACGTCATGATGTGGAAACCTGGCATAAAGATGTGCGCTTCTTTGACTTAATTGATGAAAATGATGAAGTTCGCGGCAGCTTCTATTTAGATTTGTATGCCCGTGAACATAAACGCGGCGGCGCATGGACGGACGATTGTATCGGTCGTCGCAAAACCATCGACGGTAGTCTACAAAAACCGGTCGCCTATTTAACCTGTAACTTCAATCGTCCGCTCGGTGATCAACCgg                                            >  1:655478/1‑250 (MQ=255)                                                                                                                                                                                                cATTCGTGCCGTAGAACGTCATGATGTGGAAACCTGGCATAAAGATGTGCGCTTCTTTGACTTAATTGATGAAAATGATGAAGTTCGCGGCAGCTTCTATTTAGATTTGTATGCCCGTGAACATAAACGCGGCGGCGCATGGATGGACGATTGTATCGGTCGTCGCAAAACCATCGACGGTAGTCTACAAAAACCGGTCGCCTATTTAACCTGTAACTTCAATCGTCCGCTCGGTGATCAACCGGCattat                                      >  1:589362/1‑251 (MQ=255)                                                                                                                                                                                                        ccGTAGAACGTCATGATGTGGAAACCTGGCATAAAGATGTGCGCTTCTTTGACTTAATTGATGAAAATGATGAAGTTCGCGGCAGCTTCTATTTAGATTTGTATGCCCGTGAACATAAACGCGGCGGCGCATGGATGGACGATTGTATCGGTCGTCGCAAAACCATCGACGGTAGTCTACAAAAACCGGTCGCCTATTTAACCTGTAACTTCAATCGTCCGCTCGGTGATCAACCGGCATTATTCACCCat                              >  1:785238/1‑251 (MQ=255)                                                                                                                                                                                                              aaCGTCATGATGTGGAAACCTGGCATAAAGATGTGCGCTTCTTTGACTTAATTGATGAAAATGATGAAGTTCGCGGCAGTTTCTATTTAGATTTGTATGCCCGTGAACATAAACGCGGCGGCGCATGGATGGACGATTGTATCGGTCGTCGCAAAACCATCGACGGTAGTCTACAAAAACCGGTCGCCTATTTAACCTGTAACTTCAATCGTCCGCTCGGTGATCAACCGGCATTATTCACCCatgatg                          <  1:443635/249‑1 (MQ=255)                                                                                                                                                                                                              aaCGTCATGATGTGGAAACCTGGCATAAAGATGTGCGCTTCTTTGACTTAATTGATGAAAATGATGAAGTTCGCGGCAGTTTCTATTTAGATTTGTATGCCCGTGAACATAAACGCGGCGGCGCATGGATGGACGATTGTATCGGTCGTCGCAAAACCATCGACGGTAGTCTACAAAAACCGGTCGCCTATTTAACCTGTAACTTCAATCGTCCGCTCGGTGATCAACCGGCATTATTCACCCatgatg                          <  1:456373/249‑1 (MQ=255)                                                                                                                                                                                                              aaCGTCATGATGTGGAAACCTGGCATAAAGATGTGCGCTTCTTTGACTTAATTGATGAAAATGATGAAGTTCGCGGCAGCTTCTATTTAGATTTGTATGCCCGTGAACATAAACGCGGCGGCGCATGGATGGACGATTGTATCGGTCGTCGCAAAACCATCGACGGTAGTCTACAAAAACCGGTCGCCTATTTAACCTGTAACTTCAATCGTCCGCTCGGTGATCAACCGGCATTATTCACCCatgatg                          <  1:400215/249‑1 (MQ=255)                                                                                                                                                                                                               aCGTCATGATGTGGAAACCTGGCATAAAGATGTGCGCTTCTTTGACTTAATTGATGAAAATGATGAAGTTCGCGGCAGCTTCTATTTAGATTTGTATGCCCGTGAACATAAACGCGGCGGCGCATGGATGGACGATTGTATCGGTCGTCGCAAAACCATCGACGGTAGTCTACAAAAACCGGTCGCCTATTTAACCTGTAACTTCAATCGTCCGCTCGGTGATCAACCGGCATTATTCACCCATGATGAAg                       <  1:673863/251‑1 (MQ=255)                                                                                                                                                                                                                 gTCATGATGTGGAAACCTGGCATAAAGATGTGCGCTTCTTTGACTTAATTGATGAAAATGATGAAGTTCGCGGCAGCTTCTATTTAGATTTGTATGCCCGTGAACATAAACGCGGCGGCGCATGGATGGACGATTGTATCGGTCGTCGCAAAACCATCGACGGTAGTCTACAAAAACCGGTCGCCTATTTAACCTGTAACTTCAATCGTCCGCTCGGTGATCAACCGGCATTATTCACCCATGATGAAGt                      >  1:502433/1‑250 (MQ=255)                                                                                                                                                                                                                        tgtgGAAACCTGGCATAAAGATGTGCGCTTCTTTGACTTAATTGATGAAAATGATGAAGTTCGCGGCAGCTTCTATTTAGATTTGTATGCCCGTGAACATAAACGCGGCGGCGCATGGATGGACGATTGTATCGGTCGTCGCAAAACCATCGACGGTAGTCTACAAAAACCGGTCGCCTATTTAACCTGTAACTTCAATCGTCCGCTCGGTGATCAACCGGCATTATTCACCCATGATGAAGTCACTAccc              <  1:513107/251‑1 (MQ=255)                                                                                                                                                                                                                         gtgGAAACCTGGCATAAAGATGTGCGCTTCTTTGACTTAATTGATGAAAATGATGAAGTTCGCGGCAGCTTCTATTTAGATTTGTATGCCCGTGAACATAAACGCGGCGGCGCATGGATGGACGATTGTATCGGTCGTCGCAAAACCATCGACGGTAGTCTACAAAAACCGGTCGCCTATTTAACCTGTAACTTCAATCGTc                                                              <  1:598897/202‑1 (MQ=255)                                                                                                                                                                                                                         gtgGAAACCTGGCATAAAGATGTGCGCTTCTTTGACTTAATTGATGAAAATGATGAAGTTCGCGGCAGCTTCTATTTAGATTTGTATGCCCGTGAACATAAACGCGGCGGCGCATGGATGGACGATTGTATCGGTCGTCGCAAAACCATCGACGGTAGTCTACAAAAACCGGTCGCCTATTTAACCTGTAACTTCAATCGTCCGCTCGGTGATCAACCGGCATTATTCACCCatgatg                          >  1:240589/1‑238 (MQ=255)                                                                                                                                                                                                                            gAAACCTGGCATAAAGATGTGCGCTTCTTTGACTTAATTGATGAAAATGATGAAGTTCGCGGCAGCTTCTATTTAGATTTGTATGCCCGTGAACATAAACGCGGCGGCGCATGGATGGACGATTGTATCGGTCGTCGCAAAACCATCGACGGTAGTCTACAAAAACCGGTCGCCTATTTAACCTGTAACTTCAATCGTCCGCTCGGTGATCAACCGGCATTATTCACCCATGATGAAGTCACTACCCTGt           <  1:143910/250‑1 (MQ=255)                                                                                                                                                                                                                             aaaCCTGGCATAAAGATGTGCGCTTCTTTGACTTAATTGATGAAAATGATGAAGTTCGCGGCAGCTTCTATTTAGATTTGTATGCCCGTGAACATAAACGCGGCGGCGCATGGATGGACGATTGTATCGGTCGTCGCAAAACCATCGACGGTAGTCTACAAAAACCGGTCGCCTATTTAACCTGTAACTTCAATCGTCCGCTCGGTGATCAACCGGCATTATTCACCCATGATGAAGTCACTACCCTGTTc         <  1:623971/251‑1 (MQ=255)                                                                                                                                                                                                                             aaaCCTGGCATAAAGATGTGCGCTTCTTTGACTTAATTGATGAAAATGATGAAGTTCGCGGCAGCTTCTATTTAGATTTGTATGCCCGTGAACATAAACGCGGCGGCGCATGGATGGACGATTGTATCGGTCGTCGCAAAACCATCGACGGTAGTCTACAAAAACCGGTCGCCTATTTAACCTGTAACTTCAATCGTCCGCTCGGTGATCAACCGGCATTATTCACCCATGATGAAGTCACTACCCTGTTc         <  1:589436/251‑1 (MQ=255)                                                                                                                                                                                                                                  tGGCATAAAGATGTGCGCTTCTTTGACTTAATTGATGAAAATGATGAAGTTCGCGGCAGCTTCTATTTAGATTTGTATGCCCGTGAACATAAACGCGGCGGCGCATGGATGGACGATTGTATCGGTCGTCGCAAAACCATCGACGGTAGTCTACAAAAACCGGTCGCCTATTTAACCTGTAACTTCAATCGTCCGCTCGGTGATCAACCGGCATTATTCACCCATGATGAAGTCACTACCCTGTTCCATGa    <  1:321025/251‑1 (MQ=255)                                                                                                                                                                                                                                   ggCATAAAGATGTGCGCTTCTTTGACTTAATTGATGAAAATGATGAAGTTCGCGGCAGCTTCTATTTAGATTTGTATGCCCg                                                                                                                                                                            <  1:2232/82‑1 (MQ=255)                                                                                                                                                                                                                                   ggCATAAAGATGTGCGCTTCTTTGACTTAATTGATGAAAATGATGAAGTTCGCGGCAGCTTCTATTTAGATTTGTATGCCCGTGAACATAAACGCGGCGGCGCATGGATGGACGATTGTATCGGTCGTCGCAAAACCATCGACGGTAGTCTACAAAAACCGGTCGCCTATTTAACCTGTAACTTCAATCGTCCGCTCGGTGATCAACCGGCATTATTCACCCATGATGAAGTCACTACCCTGTTCCATGaa   >  1:826904/1‑251 (MQ=255)                                                                                                                                                                                                                                    gCATAAAGATGTGCGCTTCTTTGACTTAATTGATGAAAATGATGAAGTTCGCGGCAGCTTCTATTTAGATTTGTATGCCCGTGAACATAAACGCGGCGGCGCATGGATGGACGATTGTATCGGTCGTCGCAAAACCATCGACGGTAGTCTACAAAAACCGGTCGCCTATTTAACCTGTAACTTCAATCGTCCGCTCGGTGATCAACCGGCATTATTCACCCa                               >  1:248984/1‑222 (MQ=255)                                                                                                                                                                                                                                    gCATAAAGATGTGCGCTTCTTTGACTTAATTGATGAAAATGATGAAGTTCGCGGCAGCTTCTATTTAGATTTGTATGCCCGTGAACATAAACGCGGCGGCGCATGGATGGACGATTGTATCGGTCGTCGCAAAACCATCGACGGTAGTCTACAAAAACCGGTCGCCTATTTAACCTGTAACTTCAATCGTCCGCTCGGTGATCAACCGGCATTATTCACCCATGATGAAGTCACTAccc              <  1:328607/239‑1 (MQ=255)                                                                                                                                                                                                                                    gCATAAAGATGTGCGCTTCTTTGACTTAATTGATGAAAATGATGAAGTTCGCGGCAGCTTCTATTTAGATTTGTATGCCCGTGAACATAAACGCGGCGGCGCATGGATGGACGATTGTATCGGTCGTCGCAAAACCATCGACGGTAGTCTACAAAAACCGGTCGCCTATTTAACCTGTAACTTCAATCGTCCGCTCGGTGATCAACCGGCATTATTCACCCATGATGAAGTCACTACCCTGTTCCATGAAt  >  1:147429/1‑251 (MQ=255)                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                             GAATTACAGGAATTAAAAGATTTCTGCAAAACACACTATAATTTGACCGCACTTGAGCTTTGGGATTTGTCCTTTTACAGCGAGAAACAAAAACAACATTTATATGCCATTAGCGATGAAGAATTGCGCCCTTATTTCCCGGAAGATCGTGTCCTTAACGGCTTATTTGAACTGATTAAACGCATTTTCAACATTCGTGCCGTAGAACGTCATGATGTGGAAACCTGGCATAAAGATGTGCACTTCTTTGACTTAATTGATGAAAATGATGAAGTTCGCGGCAGCTTCTATTTAGATTTGTATGCCCGTGAACATAAACGCGGCGGCGCATGGATGGACGATTGTATCGGTCGTCGCAAAACCATCGACGGTAGTCTACAAAAACCGGTCGCCTATTTAACCTGTAACTTCAATCGTCCGCTCGGTGATCAACCGGCATTATTCACCCATGATGAAGTCACTACCCTGTTCCATGAAT  >  1/1635891‑1636368

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑