Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,618,037	C→A	intergenic (‑173409/‑23727)	unknown ← / → unknown	tRNA‑Met‑CAT/tRNA‑Gly‑GCC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,618,037	0	C	A	100.0%	171.9 / NA	64	intergenic (‑173409/‑23727)	unknown/unknown	tRNA‑Met‑CAT/tRNA‑Gly‑GCC
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (29/35);  total (29/35)

CGTTCGCATAGCCAAATGCCGATGAAGAAGCAAAGTGCGGTCAAAATAAAAAATGTTTTTGCGCCGAGTACCGGCAGCAATAACGCGCCTAAAATCACGCCGACCAAACTGCCCCAAGTGCCGGGCGCGGGACGCAATAAACCGGAGCCGAACCCCAGCGCGAAAAAATGAATAGGATTGCGCAAATTCACCCGTTCGAGCGGATGTTGTAATGACGTCATAAGTGAAACCTATTTGAAATGCTCAAAACCGTATGGCAACTCAAGCTCGACCGCCAGCCCGTTGCGTTGAAACTGAATACGTTTTTTGCCCGCGTGACGAATTTGCCCGATACAGGTATATGGCACGCCGAGATGCGCCAGGGCTTTTTCTAATTTGACGATATTGTTGTCAGGTACGGTGAAACAAAGTTCGTAGTCTTCGCCGCCGGACAAGGCAAACTGTTCCGCTTTCGCCAAACCGACTTCGGCGACCAACTC  >  1/1617795‑1618273                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                             cGTTCGCATAGCCAAATGCCGATGAAGAAGCAAAGTGCGGTCAAAATAAAAAATGTTTTTGCGCCGAGTACCGGCAGCAATAACGCGCCTAAAATCACGCCGACCAAACTGCCCCAAGTGCCGGGCGCGGGACGCAATAAACCGGAGCCGAACCCCAGCGCGAAAAAATGAATAGGATTGCGCAAATTCACCCGTTCGAGCGGATGTTGTAATGACGTCATAAGTGAAACCTATTTGAAATGATCAAAAc                                                                                                                                                                                                                                       <  1:499751/250‑1 (MQ=255) cGTTCGCATAGCCAAATGCCGATGAAGAAGCAAAGTGCGGTCAAAATAAAAAATGTTTTTGCGCCGAGTACCGGCAGCAATAACGCGCCTAAAATCACGCCGACCAAACTGCCCCAAGTGCCGGGCGCGGGACGCAATAAACCGGAGCCGAACCCCAGCGCGAAAAAATGAATAGGATTGCGCAAATTCACCCGTTCGAGCGGATGTTGTAATGACGTCATAAGTGAAACCTATTTGAAATGATCAAAAc                                                                                                                                                                                                                                       <  1:468030/250‑1 (MQ=255)    tCGCATAGCCAAATGCCGATGAAGAAGCAAAGTGCGGTCAAAATAAAAAATGTTTTTGCGCCGAGTACCGGCAGCAATAACGCGCCTAAAATCACGCCGACCAAACTGCCCCAAGTGCCGGGCGCGGGACGCAATAAACCGGAGCCGAACCCCAGCGCGAAAAAATGAATAGGATTGCGC‑AATTCACCCGTTCGAGCGGATGTTGTAATGACGTCATAAGTGAAACCTATTTGAAATGATCAAAAc                                                                                                                                                                                                                                       <  1:696503/246‑1 (MQ=255)        aTAGCCAAATGCCGATGAAGAAGCAAAGTGCGGTCAAAATAAAAAATGTTTTTGCGCCGAGTACCGGCAGCAATAACGCGCCTAAAATCACGCCGACCAAACTGCCCCAAGTGCCGGGCGCGGGACGCAATAAACCGGAGCCGAACCCCAGCGCGAAAAAATGAATAGGATTGCGCAAATTCACCCGTTCGAGCGGATGTTGTAATGACGTCATAAGTGAAACCTATTTGAAATGATCAAAACCGTATGGc                                                                                                                                                                                                                               <  1:604847/251‑1 (MQ=255)           gCCAAATGCCGATGAAGAAGCAAAGTGCGGTCAAAATAAAAAATGTTTTTGCGCCGAGTACCGGCAGCAATAACGCGCCTAAAATCACGCCGACCAAACTGCCCCAAGTGCCGGGCGCGGGACGCAATAAACCGGAGCCGAACCCCAGCGCGAAAAAATGAATAGGATTGCGCAAATTCACCCGTTCGAGCGGATGTTGTAATGACGTCATAAGTGAAACCTATTTGAAATGATCAAAAc                                                                                                                                                                                                                                       >  1:644747/1‑240 (MQ=255)           gCCAAATGCCGATGAAGAAGCAAAGTGCGGTCAAAATAAAAAATGTTTTTGCGCCGAGTACCGGCAGCAATAACGCGCCTAAAATCACGCCGACCAAACTGCCCCAAGTGCCGGGCGCGGGACGCAATAAACCGGAGCCGAACCCCAGCGCGAAAAAATGAATAGGATTGCGCAAATTCACCCGTTCGAGCGGATGTTGTAATGACGTCATAAGTGAAACCTATTTGAAATGATCAAAAc                                                                                                                                                                                                                                       >  1:661066/1‑240 (MQ=255)                   ccGATGAAGAAGCAAAGTGCGGTCAAAATAAAAAATGTTTTTGCGCCGAGTACCGGCAGCAATAACGCGCCTAAAATCACGCCGACCAAACTGCCCCAAGTGCCGGGCGCGGGACGCAATAAACCGGAGCCGAACCCCAGCGCGAAAAAATGAATAGGATTGCGCAAATTCACCCGTTCGAGCGGATGTTGTAATGACGTCATAAGTGAAACCTATTTGAAATGATCAAAACCGTATGGCAACTCAAGCt                                                                                                                                                                                                                     <  1:840171/250‑1 (MQ=255)                             agCAAAGTGCGGTCAAAATAAAAAATGTTTTTGCGCCGAGTACCGGCAGCAATAACGCGCCTAAAATCACGCCGACCAAACTGCCCCAAGTGCCGGGCGCGGGACGCAATAAACCGGAGCCGAACCCCAGCGCGAAAAAATGAATAGGATTGCGCAAATTCACCCGTTCGAGCGGATGTTGTAGTGACGTCATAAGTGAAACCTATTTGAAATGATCAAAACCGTATGGCAACTCAAGCTCGACCGCCAg                                                                                                                                                                                                           >  1:189169/1‑250 (MQ=255)                                            aaaTAAAAAATGTTTTTGCGCCGAGTACCGGCAGCAATAACGCGCCTAAAATCACGCCGACCAAACTGCCCCAAGTGCCGGGCGCGGGACGCAATAAACCGGAGCCGAACCCCAGCGCGAAAAAATGAATAGGATTGCGCAAATTCACCCGTTCGAGCGGATGTTGTAATGACGTCATAAGTGAAACCTATTTGAAATGATCAAAACCGTATGGCAACTCAAGCTCGACCGCCAGCCCGTTGCGTTGAAAc                                                                                                                                                                                           <  1:560516/251‑1 (MQ=255)                                            aaaTAAAAAATGTTTTTGCGCCGAGTACCGGCAGCAATAACGCGCCTAAAATCACGCCGACCAAACTGCCCCAAGTGCCGGGCGCGGGACGCAATAAACCGGAGCCGAACCCCAGCGCGAAAAAATGAATAGGATTGCGCAAATTCACCCGTTCGAGCGGATGTTGTAATGACGTCATAAGTGAAACCTATTTGAAATGATCAAAACCGTATGGCAACTCAAGCTCGACCGCCAGCCCGTTGCGTTGAAAc                                                                                                                                                                                           <  1:19689/251‑1 (MQ=255)                                                 aaaaaTGTTTTTGCGCCGAGTACCGGCAGCAATAACGCGCCTAAAATCACGCCGACCAAACTGCCCCAAGTGCCGGGCGCGGGACGCAATAAACCGGAGCCGAACCCCAGCGCGAAAAAATGAATAGGATTGCGCAAATTCACCCGTTCGAGCGGATGTTGTAATGACGTCATAAGTGAAACCTATTTGAAATGATCAAAACCGTATGGCAACTCAAGCTCGACCGCCAGCCCGTTGCGTTGAAACTGAAt                                                                                                                                                                                      <  1:475946/251‑1 (MQ=255)                                                           ttGCGCCGAGTACCGGCAGCAATAA‑‑CGCCTAAAATCACGCCGACCAAACTGCCCCAAGTGCCGGGCGCGGGACGCAATAAACCGGAGCCGAACCCCAGCGCGAAAAAATGAATAGGATTGCGCAAATTCACCCGTTCGAGCGGATGTTGTAATGACGTCATAAGTGAAACCTATTTGAAATGATCAAAACCGTATGGCAACTCAAGCTCGACCGCCAGCCCGTTGCGTTGAAACTGAATACGTTTTTTGcc                                                                                                                                                                          <  1:346868/251‑1 (MQ=255)                                                                  gAGTACCGGCAGCAATAACGCGCCTAAAATCACGCCGACCAAACTGCCCCAAGTGCCGGGCGCGGGACGCAATAAACCGGAGCCGAACCCCAGCGCGAAAAAATGAATAGGATTGCGCAAATTCACCCGTTCGAGCGGATGTTGTAATGACGTCATAAGTGAAACCTATTTGAAATGATCAAAACCGTATGGCAACTCAAGCTCGACCGCCAGCCCGTTGCGTTGAAACTGAATACGTTTTTTGCCCGCGt                                                                                                                                                                     >  1:461183/1‑251 (MQ=255)                                                                  gAGTACCGGCAGCAATAACGCGCCTAAAATCACGCCGACCAAACTGCCCCAAGTGCCGGGCGCGGGACGCAATAAACCGGAGCCGAACCCCAGCGCGAAAAAATGAATAGGATTGCGCAAATTCACCCGTTCGAGCGGATGTTGTAATGACGTCATAAGTGAAACCTATTTGAAATGATCAAAACCGTATGGCAACTCAAGCTCGACCGCCAGCCCGTTGCGTTGAAACTGAATACGTTTTTTGCCCGCGt                                                                                                                                                                     >  1:39191/1‑251 (MQ=255)                                                                   aGTACCGGCAGCAATAACGCGCCTAAAATCACGCCGACCAAACTGCCCCAAGTGCCGGGCGCGGGACGCAATAAACCGGAGCCGAACCCCAGCGCGAAAAAATGAATAGGATTGCGCAAATTCACCCGTTCGAGCGGATGTTGTAATGACGTCATAAGTGAAACCTATTTGAAATGATCAAAAc                                                                                                                                                                                                                                       >  1:671847/1‑184 (MQ=255)                                                                       ccGGCAGCAATAACGCGCCTAAAATCACGCCGACCAAACTGCCCCAAGTGCCGGGCGCGGGACGCAATAAACCGGAGCCGAACCCCAGCGCGAAAAAATGAATAGGATTGCGCAAATTCACCCGTTCGAGCGGATGTTGTAATGACGTCATAAGTGAAACCTATTTGAAATGATCAAAACCGTATGGCAACTCAAGCTCGACCGCCAGCCCGTTGCGTTGAAACTGAATACGTTTTTTGCCCGCGTGACGa                                                                                                                                                                >  1:673551/1‑251 (MQ=255)                                                                                    cgcgCCTAAAATCACGCCGACCAAACTGCCCCAAGTGCCGGGCGCGGGACGCAATAAACCGGAGCCGAACCCCAGCGCGAAAAAATGAATAGGATTGCGCAAATTCACCCGTTCGAGCGGATGTTGTAATGACGTCATAAGTGAAACCTATTTGAAATGATCAAAACCGTATGGCAACTCAAGCTCGACCGCCAGCCCGTTGCGTTGAAACTGAATACGTTTTTTGCCCGCGTGACGAATTTGCCCGATAc                                                                                                                                                   <  1:211288/251‑1 (MQ=255)                                                                                              aTCACGCCGACCAAACTGCCCCAAGTGCCGGGCGCGGGACGCAATAAACCGGAGCCGAACCCCAGCGCGAAAAAATGAATAGGATTGCGCAAATTCACCCGTTCGAGCGGATGTTGTAATGACGTCATAAGTGAAACCTATTTGAAATGATCAAAACCGTATGGCAACTCAAGCTCGACCGCCAGCCCGTTGCGTTGAAACTGAATACGTTTTTTGCCCGCGTGACGAATTTGCCCGATACAGGTATATgg                                                                                                                                         <  1:319052/251‑1 (MQ=255)                                                                                              aTCACGCCGACCAAACTGCCCCAAGTGCCGGGCGCGGGACGCAATAAACCGGAGCCGAACCCCAGCGCGAAAAAATGAATAGGATTGCGCAAATTCACCCGTTCGAGCGGATGTTGTAATGACGTCATAAGTGAAACCTATTTGAAATGATCAAAACCGTATGGCAACTCAAGCTCGACCGCCAGCCCGTTGCGTTGAAACTGAATACGTTTTTTGCCCGCGTGACGAATTTGCCCGATACAGGTATATgg                                                                                                                                         <  1:900055/251‑1 (MQ=255)                                                                                              aTCACGCCGACCAAACTGCCCCAAGTGCCGGGCGCGGGACGCAATAAACCGGAGCCGAACCCCAGCGCGAAAAAATGAATAGGATTGCGCAAATTCACCCGTTCGAGCGGATGTTGTAATGACGTCATAAGTGAAACCTATTTGAAATGATCAAAACCGTATGGCAACTCAAGCTCGACCGCCAGCCCGTTGCGTTGAAACTGAATACGTTTTTTGCCCGCGTGACGAATTTGCCCGATACAGGTATATgg                                                                                                                                         <  1:375388/251‑1 (MQ=255)                                                                                              aTCACGCCGACCAAACTGCCCCAAGTGCCGGGCGCGGGACGCAATAAACCGGAGCCGAACCCCAGCGCGAAAAAATGAATAGGATTGCGCAAATTCACCCGTTCGAGCGGATGTTGTAATGACGTCATAAGTGAAACCTATTTGAAATGATCAAAACCGTATGGCAACTCAAGCTCGACCGCCAGCCCGTTGCGTTGAAACTGAATACGTTTTTTGCCCGCGTGACGAATTTGCCCGATACAGGTATATgg                                                                                                                                         <  1:25736/251‑1 (MQ=255)                                                                                              aTCACGCCGACCAAACTGCCCCAAGTGCCGGGCGCGGGACGCAATAAACCGGAGCCGAACCCCAGCGCGAAAAAATGAATAGGATTGCGCAAATTCACCCGTACGAGCGGATGTTGTAATGACGTCATAAGTGAAACCTATTTGAAATGATCAAAACCGTATGGCAACGCAAGCTCGACCGCCAGCCCGTTGCGTTGAAACTGAATACGTTTTTTGCCCGCGTGACGAATTTGCCCGATACAGGTATATgg                                                                                                                                         <  1:259194/251‑1 (MQ=255)                                                                                               tCACGCCGACAAAACTGCCCCAAGTGCCGGGCGCGGGACGCAATAAACCGGAGCCGAACCCCAGCGCGAAAAAATGAATAGGATTGCGCAAATTCACCCGTTCGAGCGGATGTTGTAATGACGTCATAAGTGAAACCTATTTGAAATGATCAAAACCGTATGGCAACTCAAGCTCGACCGCCAGCCCGTTGCGTTGAAACTGAATACGTTTTTTGCCCGCGTGACGAATTTGCCCGATACAGGTATATGGc                                                                                                                                        <  1:660273/251‑1 (MQ=255)                                                                                                       aCCAAACTGCCCCAAGTGCCGGGCGCGGGACGCAATAAACCGGAGCCGAACCCCAGCGCGAAAAAATGAATAGGATTGCGCAAATTCACCCGTTCGAGCGGATGTTGTAATGACGTCATAAGTGAAACCTATTTGAAATGATCAAAACCGTATGGCAACTCAAGCTCGACCGCCAGCCCGTTGCGTTGAAACTGAATACGTTTTTTGCCCGCGTGACGAATTTGCCCGATACAGGTATATgg                                                                                                                                         >  1:487633/1‑242 (MQ=255)                                                                                                         cAAACTGCCCCAAGTGCCGGGCGCGGGACGCAATAAACCGGAGCCGAACCCCAGCGCGAAAAAATGAATAGGATTGCGCAAATTCACCCGTTCGAGCGGATGTTGTAATGACGTCATAAGTGAAACCTATTTGAAATGATCAAAACCGTATGGCAACTCAAGCTCGACCGCCAGCCCGTTGCGTTGAAACTGAATACGTTTTTTGCCCGCGTGACGAATTTGCCCGATACAGGTATATGGCACGCCGAGAt                                                                                                                              <  1:468778/251‑1 (MQ=255)                                                                                                                 cccAAGTGCCGGGCGCGGGACGCAATAAACCGGAGCCGAACCCCAGCGCGAAAAAATGAATAGGATTGCGCAAATTCACCCGTTCGAGCGGATGTTGTAATGACGTCATAAGTGAAACCTATTTGAAATGATCAAAACCGTATGGCAACTCAAGCTCGACCGCCAGCCCGTTGCGTTGAAACTGAATACGTTTTTTGCCCGCGTGACGAATTTGCCCGATACAGGTATATGGCACGCCGAGATGCGCCAgg                                                                                                                      >  1:741851/1‑251 (MQ=255)                                                                                                                   cAAGTGCCGGGCGCGGGACGCAATAAACCGGAGCCGAACCCCAGCGCGAAAAAATGAATAGGATTGCGCAAATTCACCCGTGCGAGCGGATGTTGTAATGACGTCATAAGTGAAACCTATTTGAAATGATCAAAACCGTATGGCAACTCAAGCTCGACCGCCAGCCCGTTGCGTTGAAACTGAATACGTTTTTTGCCCGCGTGACGAATTTGCCCGATACAGGTATATGGCACGCCGAGATGCGCCAGGGc                                                                                                                    <  1:445238/251‑1 (MQ=255)                                                                                                                      gTGCCGGGCGCGGGACGCAATAAACCGGAGCCGAACCCCAGCGCGAAAAAATGAATAGGATTGCGCAAATTCACCCGTTCGAGCGGATGTTGTAATGACGTCATAAGTGAAACCTATTTGAAATGATCAAAACCGTATGGCAACTCAAGCTCGACCGCCAGCCCGTTGCGTTGAAACTGAATACGTTTTTTGCCCGCGTGACGAATTTGCCCGATACAGGTATATGGCACGCCGAGATGCGCCAGGGCttt                                                                                                                 >  1:760815/1‑251 (MQ=255)                                                                                                                      gTGCCGGGCGCGGGACGCAATAAACCGGAGCCGAACCCCAGCGCGAAAAAATGAATAGGATTGCGCAAATTCACCCGTTCGAGCGGATGTTGTAATGACGTCATAAGTGAAACCTATTTGAAATGATCAAAACCGTATGGCAACTCAAGCTCGACCGCCAGCCCGTTGCGTTGAAACTGAATACGTTTTTTGCCCGCGTGACGAATTTGCCCGATACAGGTATATGGCACGCCGAGATGCGCCAGGGCttt                                                                                                                 >  1:586866/1‑251 (MQ=255)                                                                                                                        gCCGGGCGCGGGACGCAATAAACCGGAGCCGAACCCCAGCGCGAAAAAATGAATAGGATTGCGCAAATTCACCCGTTCGAGCGGATGTTGTAATGACGTCATAAGTGAAACCTATTTGAAATGATCAAAACCg                                                                                                                                                                                                                                     >  1:172996/1‑133 (MQ=255)                                                                                                                                       cAATAAACCGGAGCCGAACCCCAGCGCGAAAAAATGAATAGGATTGCGCAAATTCACCCGTTCGAGCGGATGTTGTAATGACGTCATAAGTGAAACCTATTTGAAATGATCAAAACCGTATGGCAACTCAAGCTCGACCGCCAGCCCGTTGCGTTGAAACTGAATACGTTTTTTGCCCGCGTGACGAATTTGCCCGATACAGGTATATGGCACGCCGAGATGCGCCAGGGCTTTTTCTAATTTGACGatat                                                                                                >  1:836877/1‑251 (MQ=255)                                                                                                                                            aaCCGGGGCCGAACCCCAGCGCGAAAAAATGAATAGGATTGCACAAATTCACCCGTTCGAGCGGATGTTGTAATGACGTCATAAGTGAAACCTATTTGAAATGATCAAAGCCGTATGGCAACTCAAGCTCGACCGCCAGCCCGTTGCGTTGAAACTGAATACGTTTTTTGCCCGCGTGACGAATTTGCCCGATACAGGTATATGGCACGCCGAGATGCGCCAGGGCTTTTTCTAATTTGACGATAttgttg                                                                                           <  1:223533/251‑1 (MQ=255)                                                                                                                                            aaCCGGAGCCGAACCCCAGCGCGAAAAAATGAATAGGATTGCGCAAATTCACCCGTTCGAGCGGATGTTGTAATGACGTCATAAGTGAAACCTATTTGAAATGATCAAAACCGTATGGCAACTCAAGCTCGACCGCCAGCCCGTTGCGTTGAAACTGAATACGTTTTTTGCCCGCGTGACGAATTTGCCCGATACAGGTATATgg                                                                                                                                         >  1:55056/1‑205 (MQ=255)                                                                                                                                            aaCCGGAGCCGAACCCCAGCGCGAAAAAATGAATAGGATTGCGCAAATTCACCCGTTCGAGCGGATGTTGTAATGACGTCATAAGTGAAACCTATTTGAAATGATCAAAACCGTATGGCAACTCAAGCTCGACCGCCAGCCCGTTGCGTTGAAACTGAATACGTTTTTTGCCCGCGTGACGAATTTGCCCGATACAGGTATATGGCACGCCGAGATGCGCCAGGGCTTTTTCTAATTTGACGATAttgttg                                                                                           <  1:664874/251‑1 (MQ=255)                                                                                                                                                         ccccAGCGCGAAAAAATGAATAGGATTGCGCAAATTCACCCGTTCGAGCGGATGTTGTAATGACGTCATAAGTGAAACCTATTTGAAATGATCAAAACCGTATGGCAACTCAAGCTCGACCGCCAGCCCGTTGCGTTGAAACTGAATACGTTTTTTGCCCGCGTGACGAATTTGCCCGATACAGGTATATGGCACGCCGAGATgc                                                                                                                            >  1:191225/1‑205 (MQ=255)                                                                                                                                                               cgcgAAAAAATGAATAGGATTGCGCAAATTCACCCGTTCGAGCGGATGTTGTAATGACGTCATAAGTGAAACCTATTTGAAATGATCAAAACCGTATGGCAACTCAAGCTCGACCGCCAGCCCGTTGCGTTGAAACTGAATACGTTTTTTGCCCGCGTGACGAATTTGCCCGATACAGGTATATGGCACGCCGAGATGCGCCAGGGCTTTTTCTAATTTGACGATATTGTTGTCAGGTACGGTGAAACaa                                                                         <  1:747452/250‑1 (MQ=255)                                                                                                                                                                 cgAAAAAATGAATAGGATTGCGCAAATTCACCCGTTCGAGCGGATGTTGTAATGACGTCATAAGTGAAACCTATTTGAAATGATCAAAACCGTATGGCAACTCAAGCTCGACCGCCAGCCCGTTGCGTTGAAACTGAATACGTTTTTTGCCCGCGTGACGAATTTGCCCGATACAGGTATATGGCACGCCGAGATGCGCCAGGGCTTTTTCTAATTTGACGATATTGTTGTCAGGTACGGTGAAACAAAg                                                                       <  1:476720/250‑1 (MQ=255)                                                                                                                                                                 cgAAAAAATGAATAGGATTGCGCAAATTCACCCGTTCGAGCGGATGTTGTAATGACGTCATAAGTGAAACCTATTTGAAATGATCAAAACCGTATGGCAACTCAAGCTCGACCGCCAGCCCGTTGCGTTGAAACTGAATACGTTTTTTGCCCGCGTGACGAATTTGCCCGATACAGGTATATGGCACGCCGAGATGCGCCAGGGCTTTTTCTAATTTGACGATATTGTTGTCAGGTACGGTGAAACAAAg                                                                       <  1:333770/250‑1 (MQ=255)                                                                                                                                                                        aTGAATAGGATTGCGCAAATTCGCCCGTTCGAGCGGATGTTGTAATGACGTCATAAGTGAAACCTATTTGAAATGATCAAAACCGTATGGCAACTCAAGCTCGACCGCCAGCCCGTTGCGTTGAAACTGAATACGt                                                                                                                                                                                  <  1:492905/136‑1 (MQ=255)                                                                                                                                                                        aTGAATAGGATTGCGCAAATTCACCCGTTCGAGCGGATGTTGTAATGACGTCATAAGTGAAACCTATTTGAAATGATCAAAACCGTATGGCAACTCAAGCTCGACCGCCAGCCCGTTGCGTTGAAACTGAATACGTTTTTTGCCCGCGTGACGAATTTGCCCGATACAGGTATATGGCACGCCGAGATGCGCCAGGGCTTTTTCTAATTTGACGATATTGTTGTCAGGTACGGTGAAACAAAGTTCGTAGt                                                               >  1:350670/1‑251 (MQ=255)                                                                                                                                                                        aTGAATAGGATTGCGCAAATTCACCCGTTCGAGCGGATGTTGTAATGACGTCATAAGTGAAACCTATTTGAAATGATCAAAACCGTATGGCAACTCAAGCTCGACCGCCAGCCCGTTGCGTTGAAACTGAATACGTTTTTTGCCCGCGTGACGAATTTGCCCGATACAGGTATATGGCACGCCGAGATGCGCCAGGGCTTTTTCTAATTTGACGATATTGTTGTCAGGTACGGTGAAACAAAGTTCGTAGt                                                               >  1:494236/1‑251 (MQ=255)                                                                                                                                                                          gAATAGGATTGCGCAAATTCACCCGTTCGAGCGGATGTTGTAATGACGTCATAAGTGAAACCTATTTGAAATGATCAAAACCGTATGGCAACTCAAGCTCGACCGCCAGCCCGTTGCGTTGAAACTGAATACGTTTTTTGCCCGCGTGACGAATTTGCCCGATACAGGta                                                                                                                                              <  1:166991/170‑1 (MQ=255)                                                                                                                                                                          gAATAGGATTGCGCAAATTCACCCGTTCGAGCGGATGTTGTAATGACGTCATAAGTGAAACCTATTTGAAATGATCAAAACCGTATGGCAACTCAAGCTCGACCGCCAGCCCGTTGCGTTGAAACTGAATACGTTTTTTGCCCGCGTGACGAATTTGCCCGATACAGGTATATGGCACGCCGAGATGCGCCAGGGCTTTTTCTAATTTGACGATATTGTTGTCAGGTACGGTGAAACAAAGTTCGTAGt                                                               >  1:556538/1‑249 (MQ=255)                                                                                                                                                                               ggATTGCGCAAATTCACCCGTTCGAGCGGATGTTGTAATGACGTCATAAGTGAAACCTATTTGAAATGATCAAAACCGTATGGCAACTCAAGCTCGACCGCCAGcc                                                                                                                                                                                                         >  1:838626/1‑106 (MQ=255)                                                                                                                                                                               ggATTGCGCAAATTCACCCGTTCGAGCGGATGTTGTAATGACGTCATAAGTGAAACCTATTTGAAATGATCAAAACCGTATGGCAACTCAAGCTCGACCGCCAGCCCGTTGCGTTGAAACTGAATACGTTTTTTGCCCGCGTGACGAATTTGCCCGATACAGGTATATGGCACGCCGAGATGCGCCAGGGCTTTTTCTAATTTGACGATATTGTTGTCAGGTACGGTGAAACAAAGTTCGTAGTc                                                              >  1:739772/1‑245 (MQ=255)                                                                                                                                                                                    gcgcAAATTCACCCGTTCGAGCGGATGTTGTAATGACGTCATAAGTGAAACCTATTTGAAATGATCAAAACCGTATGGCAACTCAAGCTCGACCGCCAGCCCGTTGCGTTGAAACTGAATACGTTTTTTGCCCGCGTGACGAATTTGCCCGATACAGGta                                                                                                                                              >  1:226951/1‑160 (MQ=255)                                                                                                                                                                                             cACCCGTTCGAGCGGATGTTGTAATGACGTCATAAGTGAAACCTATTTGAAATGATCAAAACCGTATGGCAACTCAAGCTCGACCGCCAGCCCGTTGCGTTGAAACTGAATACGTTTTTTGCCCGCGTGACGAATTTGCCCGATACAGGTATATgg                                                                                                                                         <  1:329595/156‑1 (MQ=255)                                                                                                                                                                                                 cGTTCGAGCGGATGTTGTAATGACGTCATAAGTGAAACCTATTTGAAATGATCAAAACCGTATGGCAACTCAAGCTCGACCGCCAGCCCGTTGCGTTGAAACTGAATACGTTTTTTGCCCGCGTGACGAATTTGCCCGATACAGGTATATGGCACGCCGAGATGCGCCAGGGCTTTTTCTAATTTGACGATATTGTTGTCAGGTACGGTGAAACAAAGTTCGTAGTCTTCGCCGCCGGACAAGGCAAACTg                                      <  1:206319/251‑1 (MQ=255)                                                                                                                                                                                                  gTTCGAGCGGATGTTGTAATGACGTCATAAGTGAAACCTATTTGAAATGATCAAAACCGTATGGCAACTCAAGCTCGACCGCCAGCCCGTTGCGTTGAAACTGAATACGTTTTTTGCCCGCGTGACGAATTTGCCCGATACAGGta                                                                                                                                              <  1:311937/146‑1 (MQ=255)                                                                                                                                                                                                     cGAGCGGATGTTGTAATGACGTCATAAGTGAAACCTATTTGAAATGATCAAAACCGTATGGCAACTCAAGCTCGACCGCCAGcc                                                                                                                                                                                                         >  1:303713/1‑84 (MQ=255)                                                                                                                                                                                                              gttgtAATGACGTCATAAGTGAAACCTATTTGAAATGATCAAAACCGTATGGCAACTCAAGCTCGACCGCCAGCCCGTTGCGTTGAAACTGAATACGTTTTTTGCCCGCGTGACGAATTTGCCCGATACAGGTATATGGCACGCCGAGATGCGCCAGGGCTTTTTCTAATTTGACGATATTGTTGTCAGGTACGGTGAAACAAAGTTCGTAGTCTTCGCCGCCGGAc                                                 >  1:559096/1‑227 (MQ=255)                                                                                                                                                                                                              gttgtAATGACGTCATAAGTGAAACCTATTTGAAATGATCAAAACCGTATGGCAACTCAAGCTCGACCGCCAGCCCGTTGCGTTGAAACTGAATACGTTTTTTGCCCGCGTGACGAATTTGCCCGATACAGGTATATGGCACGCCGAGATGCGCCAGGGCTTTTTCTAATTTGACGATATTGTTGTCAGGTACGGTGAAACAAAGTTCGTAGTCTTCGCCGCCGGACAAGGCAAACTGTTCCGCTTTCGc                          >  1:731605/1‑250 (MQ=255)                                                                                                                                                                                                              gttgtAATGACGTCATAAGTGAAACCTATTTGAAATGATCAAAACCGTATGGCAACTCAAGCTCGACCGCCAGCCCGTTGCGTTGAAACTGAATACGTTTTTTGCCCGCGTGACGAATTTGCCCGATACAGGTATATGGCACGCCGAGATGCGCCAGGGCTTTTTCTAATTTGACGATATTGTTGTCAGGTACGGTGAAACAAAGTTCGTAGTCTTCGCCGCCGGACAAGGCAAACTGTTCCGCTTTCGc                          >  1:86550/1‑250 (MQ=255)                                                                                                                                                                                                               ttgtAATGACGTCATAAGTGAAACCTATTTGAAATGATCAAAACCGTATGGCAACTCAAGCTCGACCGCCAGCCCGTTGCGTTGAAACTGAATACGTTTTTTGCCCGCGTGACGAATTTGCCCGATACAGGTATATGGCACGCCGAGATGCGCCAGGGCTTTTTCTAATTTGACGATATTGTTGTCAGGTACgg                                                                                 <  1:128355/194‑1 (MQ=255)                                                                                                                                                                                                               ttgtAATGACGTCATAAGTGAAACCTATTTGAAATGATCAAAACCGTATGGCAACTCAAGCTCGACCGCCAGCCCGTTGCGTTGAAACTGAATACGTTTTTTGCCCGCGTGACGAATTTGCCCGATACAGGTATATGGCACGCCGAGATGCGCCAGGGCTTTTTCTAATTTGACGATATTGTTGTCAGGTACGGTGAAACAAAGTTCGTAGTCTTCGCCGCCGGACAAGGCAAACTGTTCCGCTTTCGCCa                        >  1:405256/1‑251 (MQ=255)                                                                                                                                                                                                                 gtAATGACGTCATAAGTGAAACCTATTTGAAATGATCAAAACCGTATGGCAACTCAAGCTCGACCGCCAGCCCGTTGCGTTGAAACTGAATACGTTTTTTGCCCGCGTGACGAATTTGCCCGATACAGGTATATGGCACGCCGAGATGCGCCAGGGCTTTTTCTAATTTGACGATATTGTTGTCAGGTACGGTGAAAc                                                                           <  1:465638/198‑1 (MQ=255)                                                                                                                                                                                                                        cGTCATAAGTGAAACCTATTTGAAATGATCAAAACCGTATGGCAACTCAAGCTCGACCGCCAGCCCGTTGCGTTGAAACTGAATACGTTTTTTGCCCGCGTGACGAATTTGCCCGATACAGGTATATGGCACGCCGAGATGCGCCAGGGCTTTTTCTAATTTGACGATATTGTTGTCAGGTACGGTGAAACAAAGTTCGTAGTCTTCGCCGCCGGACAAGGCAAACTGTTCCGCtt                              <  1:333875/236‑1 (MQ=255)                                                                                                                                                                                                                        cGTCATAAGTGAAACCTATTTGAAATGATCAAAACCGTATGGCAACTCAAGCTCGACCGCCAGCCCGTTGCGTTGAAACTGAATACGTTTTTTGCCCGCGTGACGAATTTGCCCGATACAGGTATATGGCACGCCGAGATGCGCCAGGGCTTTTTCTAATTTGACGATATTGTTGTCAGGTACGGTGAAACAAAGTTCGTAGTCTTCGCCGCCGGACAAGGCAAACTGTTCCGCTTTCGCCAAACCGACtt               >  1:246833/1‑251 (MQ=255)                                                                                                                                                                                                                        cGTCATAAGTGAAACCTATTTGAAATGATCAAAACCGTATGGCAACTCAAGCTCGACCGCCAGCCCGTTGCGTTGAAACTGAATACGTTTTTTGCACGCGTGACGAATTTGCCCGATACAGGTATATGGCACGCCGAGATGCGCCAGGGCTTTTTCTAATTTGACGATATTGTTGTCAGGTACGGTGAAACAAAGTTCGTAGTCTTCGCCGCCGGACAAGGCAAACTGTTCCGCTTTCGCCAAACCGACtt               >  1:835463/1‑251 (MQ=255)                                                                                                                                                                                                                        cGTCATAAGTGAAACCTATTTGAAATGATCAAAACCGTATGGCAACTCAAGCCCGACCGCCAGCCCGTTGCGTTGAAACTGAATACGTTTTTTGCCCGCGTGACGAATTTGCCCGATACAGGTATATgg                                                                                                                                         <  1:790787/129‑1 (MQ=255)                                                                                                                                                                                                                               aGTGAAACCTATTTGAAATGATCAAAACCGTATGGCAACTCAAGCTCGACCGCCAGCCCGTTGCGTTGAAACTGAATACGTTTTTTGCCCGCGTGACGAATTTGCCCGATACAGGTATATGGCAc                                                                                                                                      >  1:123893/1‑125 (MQ=255)                                                                                                                                                                                                                                     aCCTATTTGAAATGATCAAAACCGTATGGCAACTCAAGCTCGACCGCCAGCCCGTTGCGTTGAAACTGAATACGTTTTTTGCCCGCGTGACGAATTTGCCCGATACAGGTATATGGCACGCCGAGATGCGCCAGGGCTTTTTCTAATTTGACGATATTGTTGTCAGGTACGGTGAAACAAAGTTCGTAGTCTTCGCCGCCGGACAAGGCAAACTGTTCCGCTTTCGCCAAACCGACTTCGGCGACCAACTc  <  1:345655/251‑1 (MQ=255)                                                                                                                                                                                                                                     aCCTATTTGAAATGATCAAAACCGTATGGCAACTCAAGCTCGACCGCCAGCCCGTTGCGTTGAAACTGAATACGTTTTTTGCCCGCGTGACGAATTTGCCCGATACAGGTATATGGCACGCCGAGATGCGCCAGGGCTTTTTCTAATTTGACGATATTGTTGTCAGGTACGGTGAAACAAAGTTCGTAGTCTTCGCCGCCGGACAAGGCAAACTGTTCCGCTTTCGCCAAACCGACTTCGGCGACCAACTc  <  1:89542/251‑1 (MQ=255)                                                                                                                                                                                                                                         aTTTGAAATGATCAAAACCGTATGGCAACTCAAGCTCGACCGCCAGCCCGTTGCGTTGAAACTGAATACGTTTTTTGCCCGCGTGACGAATTTGCCCGATACAGGTATATGGCACGCCGAGATGCGCCAGGGCTTTTTCTAATTTGACGATATTGTTGTCAGGTACGGTGAAAc                                                                           <  1:643122/174‑1 (MQ=255)                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                             CGTTCGCATAGCCAAATGCCGATGAAGAAGCAAAGTGCGGTCAAAATAAAAAATGTTTTTGCGCCGAGTACCGGCAGCAATAACGCGCCTAAAATCACGCCGACCAAACTGCCCCAAGTGCCGGGCGCGGGACGCAATAAACCGGAGCCGAACCCCAGCGCGAAAAAATGAATAGGATTGCGCAAATTCACCCGTTCGAGCGGATGTTGTAATGACGTCATAAGTGAAACCTATTTGAAATGCTCAAAACCGTATGGCAACTCAAGCTCGACCGCCAGCCCGTTGCGTTGAAACTGAATACGTTTTTTGCCCGCGTGACGAATTTGCCCGATACAGGTATATGGCACGCCGAGATGCGCCAGGGCTTTTTCTAATTTGACGATATTGTTGTCAGGTACGGTGAAACAAAGTTCGTAGTCTTCGCCGCCGGACAAGGCAAACTGTTCCGCTTTCGCCAAACCGACTTCGGCGACCAACTC  >  1/1617795‑1618273

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑