Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,616,718	T→C	intergenic (‑172090/‑25046)	unknown ← / → unknown	tRNA‑Met‑CAT/tRNA‑Gly‑GCC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,616,718	0	T	C	100.0%	206.7 / NA	75	intergenic (‑172090/‑25046)	unknown/unknown	tRNA‑Met‑CAT/tRNA‑Gly‑GCC
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (34/41);  total (34/41)

CCAAATTCACGCCGACACTGAAATTTGAAGCAAACACGATAGCGGTCTTTTCGGCTGCTGATTTAATTGCCGCTTTGCCGGCGTCATCAAAACCCGTGGTGCCGATAATGATTTTTTTATGGTTGGCAACACAAAATGCCAAATGTTCCAATGTGCCTT‑CCGGGCGGGTGAAATCAATCAGTAAATCGAAACGATCTTTCACCGCGTTTAAGTCATCGCCGACCTTAATGCCTAAGTTGCCGATTCCCGCCAATTCGCCGGCATCGGCGCCCACCAATGAGGAACCTTTGCGCTCAAACGCCGCACCTAATTCCACGCCTTCCGCGTTATGTACCGCCTGAATTAATTGGCGACCCATACGTCCGCCTGCGCCCACTACAGCAATTTTTAATGTCATATTGTTTCTCCCGAAAATAAATTAGCCTAACACTTTCAGCGCACCACTATAAATTAAGTAGATGCCGAATAAAATAAAAATTACGCCCGAAACG  >  1/1616474‑1616964                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                       ccAAATTCACGCCGACACTGAAATTTGAAGCAAACACGATAGCGGTCTTTTCGGCTGCCGATTTAATTGCCGCTTTGCCGGCGTCATCAAAACCCGTGGTGCCGATAACGATTTTTTTATGGTTGGCAACACAAAATGCCAAATGTTCCAATGTGCCTT‑CCGGGCGGGTGAAATCAATCAGTAAATCGAAACGATCTTTCACCGCGTTTAAGTCATCGCCGACCTTAATGCCTAAGTTGCCGATCCCCGcc                                                                                                                                                                                                                                                  <  1:383781/251‑1 (MQ=255)                             aGCAAACACGATAGCGGTCTTTTCGGCTGCCGATTTAATTGCCGCTTTGCCGGCGTCATCAAAACCCGTGGTGCCGATAACGATTTTTTTATGGTTGGCAACACAAAATGCCAAATGTTCCAATGTGCCTT‑CCGGGCGGGTGAAATCAATCAGTAAATCGAAACGATCTTTCACCGCGTTTAAGTCATCGCCGACCTTAATGCCTAAGTTGCCGATCCCCGCCAATTCGCCGGCATCGGCGCCCACCAATg                                                                                                                                                                                                                      >  1:152581/1‑251 (MQ=255)                               cAAACACGATAGCGGTCTTTTCGGCTGCCGATTTAATTGCCGCTTTGCCGGCGTCATCAAAACCCGTGGTGCCGATAACGATTTTTTTATGGTTGGCAACACAAAATGCCAAATGTTCCAATGTGCCTT‑CCGGGCGGGTGAGATCAATCAGTAAATCGAAACGATCTTTCACCGCGTTTAAGTCATCGCCGACCTTAATGCCTAAGTTGCCGATCCCCGCCAATTCGCCGGCATCGGCGCCCACCAATGa                                                                                                                                                                                                                     <  1:610461/250‑1 (MQ=255)                                aaaCACGATAGCGGTCTTTTCGGCTGCCGATTTAATTGCCGCTTTGCCGGCGTCATCAAAACCCGTGGTGCCGATAACGATTTTTTTATGGTTGGCAACACAAAATGCCAAATGTTCCAATGTGCCTT‑CCGGGCGGGTGAAATCAATCAGTAAATCGAAACGATCTTTCACCGCGTTTAAGTCATCGCCGACCTTAATGCCTAAGTTGCCGATCCCCGCCAATTCGCCGGCATCGGCGCCCACCAATGAgg                                                                                                                                                                                                                   >  1:568871/1‑251 (MQ=255)                                aaaCACGATAGCGGTCTTTTCGGCTGCCGATTTAATTGCCGCTTTGCCGGCGTCATCAAAACCCGTGGTGCCGATAACGATTTTTTTATGGTTGGCAACACAAAATGCCAAATGTTCCAATGTGCCTT‑CCGGGCGGGTGAAATCAATCAGTAAATCGAAACGATCTTTCACCGCGTTTAAGTCATCGCCGACCTTAATGCCTAAGTTGCCGATCCCCGCCAATTCGCCGGCATCGGCGCCCACCAATGAgg                                                                                                                                                                                                                   >  1:689798/1‑251 (MQ=255)                                  acacGATAGCGGTCTTTTCGGCTGCCGATTTAATTGCCGCTTTGCCGGCGTCATCAAAACCCGTGGTGCCGATAACGATTTTTTTATGGTTGGCAACACAAAATGCCAAATGTTCCAATGTGCCTT‑CCGGGCGGGTGAAATCAATCAGTAAATCGAAACGATCTTTCACCGCGTTTAAGTCATCGCCGACCTTAATGCCTAAGTTGCCGATCCCCGCCAATTCGCCGGCATCGGCGCCCACCAATGAgg                                                                                                                                                                                                                   <  1:212110/249‑1 (MQ=255)                                   cacGATAGCGGTCTTTTCGGCTGCCGATTTAATTGCCGCTTTGCCGGCGTCATCAAAACCCGTGGTGCCGATAACGATTTTTTTATGGTTGGCAACACAAAATGCCAAATGTTCCAATGTGCCTT‑CCGGGCGGGTGAAATCAATCAGTAAATCGAAACGATCTTTCACCGCGTTTAAGTCATCGCCGACCTTAATGCCTAAGTTGCCGATCCCCGCCAATTCGCCGGCATCGGCGCCCACCAATGAgg                                                                                                                                                                                                                   <  1:582097/248‑1 (MQ=255)                                   cacGATAGCGGTCTTTTCGGCTGCCGATTTAATTGCCGCTTTGCCGGCATCATCAAAACCCGTGGTGCCGATAACGATTTTTTTACGATTGGTGACACAAAATGCCAAGTGTTCCAATGTGCCTTCCCGGGCGGGTGAAATCAATCAGCAAATCGAAACGATCTTTCGCCGCGTTTAAGTCATCGCCGACCTTAATGCCTAAGTTGCCGATCCCTGCCAATTCGCCGGCATCGGCGCCCACCAATGAGGaa                                                                                                                                                                                                                 >  1:66080/1‑251 (MQ=255)                                    ccGATAGCGGTCTTTTCGGCTGCCGATTTAATTGCCGCTTTGCCGGCGTCATCAAAACCCGTGGTGCCGATAACGATTTTTTTATGGTTGGCAACACAAAATGCCAAATGTTCCAATGTGCCTT‑CCGGGCGGGTGAAATCAATCAGTAAATCGAAACGATCTTTCACCGCTTTTAAGTCATCGCCGACCTTAATGCCTAAGTTGCCGATCCCCGCCAATTCGCCGGCATCGGCGCCCACCAATGAGGAAcc                                                                                                                                                                                                               <  1:519382/250‑1 (MQ=255)                                    ccGATAGCGGTCTTTTCGGCTGCCGATTTAATTGCCGCTTTGCCGGCGTCATCAAAACCCGTGGTGCCGATAACGATTTTTTTATGGTTGGCAACACAAAATGCCAAATGTTCCAATGTGCCTT‑CCGGGCGGGTGAAATCAATCAGTAAATCGAAACGATCTTTCACCGCGTTTAAGTCATCGCCGACCTTAATGCCTAAGTTGCCGATCCCCGCCAATTCGCCGGCATCGGCGCCCACCAATGAGGAAcc                                                                                                                                                                                                               <  1:837788/250‑1 (MQ=255)                                        tAGCGGTCTTTTCGGCTGCCGATTTAATTGCCGCTTTGCCGGCGTCATCAAAACCCGTGGTGCCGATAACGATTTTTTTATGGTTGGCAACACAAAATGCCAAATGTTCCAATGTGCCTT‑CCGGGCGGGTGAAATCAATCAGTAAATCGAAACGATCTTTCTCCGCGTTTAAGTCATCGCCGACCTTAATGCCTAAGTTGCCGATCCCCGCCAATTCGCCGGCATCGGCGCCCACCAATGAGGAACCTTTg                                                                                                                                                                                                           <  1:261109/251‑1 (MQ=255)                                           aatgtgtataagaggcagATTTAATTGCCGCTTTGCGGGCGTCATCAAAACCCGTGGTGCGGATAACGATTTTTTTATGGTTGGCAACACAAAAGGCCAAATGTTCCAATGTGCCTT‑CCGGGCGGGTGAAATCAATCAGTAAATCGAAACGATCTTTCTCCGCGTTTAAGTCATCGCCGACCTTAATGCCTAAGTTGCCGATCCCCGCCAATTCGCCGGCATCGGCGCCCACCAATGAGGAACCTTTgcgc                                                                                                                                                                                                        <  1:860297/234‑1 (MQ=255)                                               cTTTTCGGCTGCCGATTTAATTGCCGCTTTGCCGGCGTCATCAAAACCCGTGGTGCCGATAACGATTTTTTTATGGTTGGCAACACAAAATGCCAAATGTTCCAATGTGCCTT‑CCGGGCGGGTGAAATCAATCAGTAAATCGAAACGATCTTTCACCGCGTTTAAGTCATCGCCGACCTTAATGCCTAAGTTGCCGATCCCCGCCAATTCGCCGGCATCGGCGCCCACCAATGAGGAACCTTTGCGCTCaa                                                                                                                                                                                                    >  1:596478/1‑251 (MQ=255)                                                            gATTTAATTGCCGCTTTGCCGGCGTCATCAAAACCCGTGGTGCCGATAACGATTTTTTTATGGTTGGCAACACAAAATGCCAAATGTTCCAATGTGCCTT‑CCGGGCGGGTGAAATCAATCAGTAAATCGAAACGATCTTTCACCGCGTTTAAGTCATCGCCGACCTTAATGCCTAAGTTGCCGATCCCCGCCAATTCGCCGGCATCGGCGCCCACCAATGAGGAACCTTTGCGCTCAAACGCCGCACCTaa                                                                                                                                                                                       >  1:16679/1‑251 (MQ=255)                                                             aTTTAATTGCCGCTTTGCCGGCGTCATCAAAACCCGTGGTGCCGATAACGATTTTTTTATGGTTGGCAACACAAAATGCCAAATGTTCCAATGTGCCTT‑CCGGGCGGGTGAAATCAATCAGTAAATCGAAACGATCTTTCACCGCGTTTAAGTCATCGCCGACCTTAATGCCTAAGTTGCCGATCCCCGCCAATTCGCCGGCATCGGCGCCCACCAATGAGGAACCTTTGCGCTCAAACGCCGCACCTAAt                                                                                                                                                                                      >  1:716172/1‑251 (MQ=255)                                                               ttAATTGCCGCTTTGCCGGCGTCATCAAAACCCGTGGTGCCGATAACGATTTTTTTATGGTTGGCAACACAAAATGCCAAATGTTCCAATGTGCCTT‑CCGGGCGGGTGAAATCAATCAGTAAATCGAAACGATCTTTCACCGCGTTTAAGTCATCGCCGACCTTAATGCCTAAGTTGCCGATCCCCGCCAATTCGCCGGCATCGGCGCCCACCAATGAGGAACCTTTGCGCTCAAACGCCGCACCTAATTc                                                                                                                                                                                    <  1:49808/251‑1 (MQ=255)                                                               ttAATTGCCGCTTTGCCGGCGTCATCAAAACCCGTGGTGCCGATAACGATTTTTTTATGGTTGGCAACACAAAATGCCAAATGTTCCAATGTGCCTT‑CCGGGCGGGTGAAATCAATCAGTAAATCGAAACGATCTTTCACCGCGTTTAAGTCATCGCCGACCTTAATGCCTAAGTTGCCGATCCCCGCCAATTCGCCGGCATCGGCGCCCACCAATGAGGAACCTTTGCGCTCAAACGCCGCACCTAATTc                                                                                                                                                                                    <  1:118957/251‑1 (MQ=255)                                                                 aaTTGCCGCTTTGCCGGCGTCATCAAAACCCGTGGTGCCGATAACGATTTTTTTATGGTTGGCAACACAAAATGCCAAATGTTCCAATGTGCCTT‑CCGGGCGGGTGAAATCAATCAGTAAATCGAAACGATCTTTCACCGCGTTTAAGTCATCGCCGACCTTAATGCCTAAGTTGCCGATCCCCGCCAATTCGCCGGCATCGGCGCCCACCAATGAGGAACCTTTGCGCTCAAACGCCGCACCTAATTCCa                                                                                                                                                                                  <  1:872901/251‑1 (MQ=255)                                                                      ccGCTTTGCCGGCGTCATCAAAACCCGTGGTGCCGATAACGATTTTTTTATGGTTGGCAACACAAAATGCCAAATGTTCCAATGTGCCTT‑CCGGGCGGGTGAAATCAATCAGTAAATCGAAACGATCTTTCACCGCGTTTAAGTCATCGCCGACCTTAATGCCTAAGTTGCCGATCCCCGCCAATTCGCCGGCATCGGCGCCCACCAATGAGGAACCTTTGCGCTCAAACGCCGCACCTAATTCCACGcc                                                                                                                                                                              <  1:896599/250‑1 (MQ=255)                                                                           ttGCCGGCGTCATCAAAACCCGTGGTGCCGATAACGATTTTTTTATGGTTGGCAACACAAAATGCCAAATGTTCCAATGTGCCTT‑CCGGGCGGGTGAAATCAATCAGTAAATCGAAACGATCTTTCACCGCGTTTAAGTCATCGCCGACCTTAATGCCTAAGTTGCCGATCCCCGCCAATTCGCCGGCATCGGCGCCCACCAATGAgg                                                                                                                                                                                                                   >  1:597447/1‑208 (MQ=255)                                                                            tGCCGGCGTCATCAAAACCCGTGGTGCCGATAACGATTTTTTTATGGTTGGCAACACAAAATGCCAAATGTTCCAATGTGCCTT‑CCGGGCGGGTGAAATCAATCAGTAAATCGAAACGATCTTTCACCGCGTTTAAGTCATCGCCGACCTTAATGCCTAAGTTGCCGATCCCCGCCAATTCGCCGGCATCGGCGCCCACCAATGAGGAACCTTTGCGCTCAAACGCCGCACCTAATTCCACGCCTTCcgcg                                                                                                                                                                       <  1:92564/251‑1 (MQ=255)                                                                              ccGGCGTCATCAAAACCCGTGGTGCCGATAACGATTTTTTTATGGTTGGCAACACAAAATGCCAAATGTTCCAATGTGCCTT‑CCGGGCGGGTGAAATCAATCAGTAAATCGAAACGATCTTTCACCGCGTTTAAGTCATCGCCGACCTTAATGCCTAAGTTGCCGATCCCCGCCAATTCGCCGGCATCGGCGCCCACCAATGAGGAACCTTTGCGCTCAAACGCCGCACCTAATTCCACGCCTTCCGCGtt                                                                                                                                                                     <  1:591739/251‑1 (MQ=255)                                                                                    tcatcaAAACCCGTGGTGCCGATAACGATTTTTTTATGGTTGGCAACACAAAATGCCAAATGTTCCAATGTGCCTT‑CCGGGTGGGTGAAATCAATCAGTAAATCGAAACGATCTTTCACCGCGTTTAAGTCATCGCCGACCTTAATGCCTAAGTTGCCGATCCCCGCCAATTCGCCGGCATCGGCGCCCACCAATGAGGAACCTTTGCGCTCAAACGCCGCACCTAATTCCACGCCTTCCGCGTTATGTAc                                                                                                                                                               <  1:432415/251‑1 (MQ=255)                                                                                    tcatcaAAACCCGTGGTGCCGATAACGATTTTTTTATGGTTGGCAACACAAAATGCCAAATGTTCCAATGTGCCTT‑CCGGGCGGGTGAAATCAATCAGTAAATCGAAACGATCTTTCACCGCGTTTAAGTCATCGCCGACCTTAATGCCTAAGTTGCCGATCCCCGCCAATTCGCCGGCATCGGCGCCCACCAATGAGGAACCTTTGCGCTCAAACGCCGCACCTAATTCCACGCCTTCCGCGTTATGTAc                                                                                                                                                               <  1:178867/251‑1 (MQ=255)                                                                                    tcatcaAAACCCGTGGTGCCGATAACGATTTTTTTATGGTTGGCAACACAAAATGCCAAATGTTCCAATGTGCCTT‑CCGGGCGGGTGAAATCAATCAGTAAATCGAAACGATCTTTCACCGCGTTTAAGTCATCGCCGACCTTAATGCCTAAGTTGCCGATCCCCGCCAATTCGCCGGCATCGGCGCCCACCAATGAGGAACCTTTGCGCTCAAACGCCGCACCTAATTCCACGCCTTCCGCGTTATGTAc                                                                                                                                                               <  1:177514/251‑1 (MQ=255)                                                                                       tcaAAACCCGTGGTGCCGATAACGATTTTTTTATGGTTGGCAACACAAAATGCCAAATGTTCCAATGTGCCTT‑CCGGGCGGGTGAAATCAATCAGTAAATCGAAACGATCTTTCACCGCGTTTAAGTCATCGCCGCCCTTAATGCCTAAGTTGCCGATCCCCGCCAATTCGCCGGCATCGGCGCCCACCAATGAGGAACCTTTGCGCTCAAACGCCGCACCTAATTCCACGCCTTCCGCGTTATGTACCGc                                                                                                                                                            <  1:496309/251‑1 (MQ=255)                                                                                        caAAACCCGTGGTGCCGATAACGATTTTTTTATGGTTGGCAACACAAAATGCCAAATGTTCCAATGTGCCTT‑CCGGGCGGGTGAAATCAATCAGTAAATCGAAACGATCTTTCACCGCGTTTAAGTCATCGCCGACCTTAATGCCTAAGTTGCCGATCCCCGCCAATTCGCCGGCATCGGCGCCCACCAATGAGGAACCTTTGCGCTCAAACGCCGCACCTAATTCCACGCCTTCCGCGTTATGTACCGcc                                                                                                                                                           <  1:691796/251‑1 (MQ=255)                                                                                             cccGTGGTGCCGATAACGATTTTTTTATGGTTGGCAACACAAAATGCCAAATGTTCCAATGTGCCTT‑CCGGGCGGGTGAAATCAATCAGTAAATCGAAACGATCTTTCACCGCGTTTAAGTCATCGCCGACCTTAATGCCTAAGTTGCCGATCCCCGCCAATTCGCCGGCATCGGCGCCCACCAATGAGGAACCTTTGCGCTCAAAcgc                                                                                                                                                                                                >  1:378035/1‑209 (MQ=255)                                                                                                               aTTTTTTTATGGTTGGCAACACAAAATGCCAAATGTTCCAATGTGCCTT‑CCGGGCGGGTGAAATCAATCAGTAAATCGAAACGATCTTTCACCGCGTTTAAGTCATCGCCGACCTTAATGCCTAAGTTGCCGATCCCCGCCAATTCGCCGGCATCGGCGCCCACCAATGAGGAACCTTTGCGCTCAAACGCCGCACCTAATTCCACGCCTTCCGCGTTATGTACCGCCTGAATTAATTGGCGACCCATACg                                                                                                                                    <  1:94020/251‑1 (MQ=255)                                                                                                                 ttttttATGGTTGGCAACACAAAATGCCAAATGTTCCAATGTGCCTT‑CCGGGCGGGTGAAATCAATCAGTAAATCGAAACGATCTTTCACCGCGTTTAAGTCATCGCCGACCTTAATGCCTAAGTTGCCGATCCCCGCCAATTCGCCGGCATCGGCGCCCACCAATGAGGAACCTTTGCGCTCAAACGCCGCACCTAATTCCACGCCTTCCGCGTTATGTACCGCCTGAATTAATTGGCGACCCATACGt                                                                                                                                   >  1:371996/1‑250 (MQ=255)                                                                                                                  tttttATGGTTGGCAACACAAAATGCCAAATGTTCCAATGTGCCTT‑CCGGGCGGGTGAAATCAATCAGTAAATCGAAACGATCTTTCACCGCGTTTAAGTCATCGCCGACCTTAATGCCTAAGTTGCCGATCCCCGCCAATTCGCCGGCATCGGCGCCCACCAATGAGGAACCTTTGCGCTCAAACGCCGCACCTAATTCCACGCCTTCCGCGTTATGTACCGCCTGAATTAATTGGCGACCCATACGTc                                                                                                                                  >  1:815869/1‑250 (MQ=255)                                                                                                                  tttttATGGTTGGCAACACAAAATGCCAAATGTTCCAATGTGCCTT‑CCGGGCGGGTGAAATCAATCAGTAAATCGAAACGATCTTTCACCGCGTTTAAGTCATCGCCGACCTTAATGCCTAAGTTGCCGATCCCCGCCAATTCGCCGGCATCGGCGCCCACCAATGAGGAACCTTTGCGCTCAAACGCCGCACCTAATTCCACGCCTTCCGCGTTATGTACCGCCTGAATTAATTGGCGACCCATACGTc                                                                                                                                  >  1:844859/1‑250 (MQ=255)                                                                                                                                 acacAAAATGCCAAATGTTCCAATGTGCCTT‑CCGGGCGGGTGAAATCAATCAGTAAATCGAAACGATCTTTCACCGCGTTTAAGTCATCGCCGACCTTAATGCCTAAGTTGCCGATCCCCGCCAATTCGCCGGCATCGGCGCCCACCAATGAGGAACCTTTGCGCTCAAACGCCGCACCTAATTCCACGCCTTCCGCGTTATGTACCGCCTGAATTAATTGGCGACCCATACGTCCGCCTGCGCCCACTAc                                                                                                                  <  1:530358/251‑1 (MQ=255)                                                                                                                                    caAAATGCCAAATGTTCCAATGTGCCTT‑CCGGGCGGGTGAAATCAATCAGTAAATCGAAACGATCTTTCACCGCGTTTAAGTCATCGCCGACCTTAATGCCTAAGTTGCCGATCCCCGCCAATTCGCCGGCATCGGCGCCCACCAATGAGGAACCTTTGCGCTCAAACGCCGCACCTAATTCCACGCCTTCCGCGTTATGTACCGCCTGAATTAATTGGCGACCCATACGTCCGCCTGCGCCCACTACAGc                                                                                                               <  1:358580/251‑1 (MQ=255)                                                                                                                                      aaaTGCCAAATGTTCCAATGTGCCTT‑CCGGGCGGGTGAAATCAATCAGTAAATCGAAACGATCTTTCACCGCGTTTAAGTCATCGCCGACCTTAATGCCTAAGTTGCCGATCCCCGCCAATTCGCCGGCATCGGCGCCCACCAATGAGGAACCTTTGCGCTCAAACGCCGCACCTAATTCCACGCCTTCCGCGTTATGTACCGCCTGAATTAATTGGCGACCCATACGTCCGCCTGCGCCCACTACAGCaa                                                                                                             <  1:637791/251‑1 (MQ=255)                                                                                                                                          gCCAAATGTTCCAATGTGCCTT‑CCGGGCGGGTGAAATCAATCAGTAAATCGAAACGATCTTTCACCGCGTTTAAGTCATCGCCGACCTTAATGCCTAAGTTGCCGATCCCCGCCAATTCGCCGGCATCGGCGCCCACCAATGAGGAACCTTTGCGCTCAAACGCCGCACCTAATTCCACGCCTTCCGCGTTATGTACCGCCTGaattaatt                                                                                                                                                 >  1:779036/1‑211 (MQ=255)                                                                                                                                             aaaTGTTCCAATGTGCCTT‑CCGGGCGGGTGAAATCAATCAGTAAATCGAAACGATCTTTCACCGCGTTTAAGTCATCGCCGACCTTAATGCCTAAGTTGCCGATCCCCGCCAATTCGCCGGCATCGGCGCCCACCAATGAGGAACCTTTGCGCTCAAACGCCGCACCTAATTCCACGCCTTCCGCGTTATGTACCGCCTGAATTAATTGGCGACCCATACGTCCGCCTgcgc                                                                                                                         >  1:666412/1‑232 (MQ=255)                                                                                                                                               aTGTTCCAATGTGCCTT‑CCGGGCGGGTGAAATCAATCAGTAAATCGAAACGATCTTTCACCGCGTTTAAGTCATCGCCGACCTTAATGCCTAAGTTGCCGATCCCCGCCAATTCGCCGGCATCGGCGCCCACCAATGAGGAACCTTTGCGCTCAAACGCCGCACCTAATTCCACGCCTTCCGCGTTATGTACCGCCTGAATTAATTGGCGACCCATACGTCCGCCTGCGCCCACTACAGCAATTTTTAAtt                                                                                                    >  1:727626/1‑250 (MQ=255)                                                                                                                                               aTGTTCCAATGTGCCTT‑CCGGGCGGGTGAAATCAATCAGTAAATCGAAACGATCTTTCACCGCGTTTAAGTCATCGCCGACCTTAATGCCTAAGTTGCCGATCCCCGCCAATTCGCCGGCATCGGCGCCCACCAATGAGGAACCTTTGCGCTCAAACGCCGCACCTAATTCCACGCCTTCCGCGTTATGTACCGCCTGAATTAATTGGCGACCCATACGTCCGCCTGCGCCCACTACAGCAATTTTTAATg                                                                                                    >  1:539813/1‑251 (MQ=255)                                                                                                                                                tGTTCCAATGTGCCTT‑CCGGGCGGGTGAAATCAATCAGTAAATCGAAACGATCTTTCACCGCGTTTAAGTCATCGCCGACCTTAATGCCTAAGTTGCCGATCCCCGCCAATTCGCCGGCATCGGCGCCCACCAATGAGGAACCTTTGCGCTCAAACGCCGCACCTAATTCCACGCCTTCCGCGTTATGTACCGCCTGAATTAATTGGCGACCCATACGTCCGCCTGCGCCCACTACAGCAATTTTTAATGt                                                                                                   <  1:285276/251‑1 (MQ=255)                                                                                                                                                 gTTCCAATGTGCCTT‑CCGGGCGGGTGAAATCAATCAGTAAATCGAAACGATCTTTCACCGCGTTTAAGTCATCGCCGACCTTAATGCCTAAGTTGCCGATCCCCGCCAATTCGCCGGCATCGGCGCCCACCAATGAGGAACCTTTGCGCTCAAACGCCGCACCTAATTCCACGCCTTCCGCGTTATGTACCGCCTGAATTAATTGGCGACCCATACGTCCGCCTGCGCCCACTACAGCaa                                                                                                             <  1:636815/240‑1 (MQ=255)                                                                                                                                                       aTGTGCCTT‑CCGGGCGGGTGAAATCAATCAGTAAATCGAAACGATCTTTCCCCGCGTTTAAGTCATCGCCGACCTTAATGCCTAAGTTGCCGATCCCCGCCAATTCGCCGGCATCGGCGCCCACCAATGAGGAACCTTTGCGCTCAAACGCCGCACCTAATTCCACGCCTTCCGCGTTATGTACCGCCTGAATTAATTGGCGACCCATACGTCCGCCTGCGCCCACTACAGCAATTTTTAATGTCATATTg                                                                                            <  1:215179/251‑1 (MQ=255)                                                                                                                                                        tgtgCCTT‑CCGGGCGGGTGAAATCAATCAGTAAATCGAAACGATCTTTCACCGCGTTTAAGTCATCGCCGACCTTAATGCCTAAGTTGCCGATCCCCGCCAATTCGCCGGCATCGGCGCCCACCAATGAGGAACCTTTGCGCTCAAACGCCGCACCTAATTCCACGCCTTCCGCGTTATGTACCGCCTGAATTAATTGGCGACCCATACGTCCGCCTg                                                                                                                            <  1:615151/218‑1 (MQ=255)                                                                                                                                                             cTT‑CCGGGCGGGTGAAATCAATCAGTAAATCGAAACGATCTTTCACCGCGTTTAAGTCATCGCCGACCTTAATGCCTAAGTTGCCGATCCCCGCCAATTCGCCGGCATCGGCGCCCACCAATGAGGAACCTTTGCGCTCAAACGCCGCACCTAATTCCACGCCTTCCGCGTTATGTACCGcc                                                                                                                                                           <  1:511319/182‑1 (MQ=255)                                                                                                                                                                        ggTGAAATCAATCAGTAAATCGAAACGATCTTTCACCGCGTTTAAGTCATCGCCGACCTTAATGCCTAAGTTGCCGATCCCCGCCAATTCGCCGGCATCGGCGCCCACCAATGAGGAACCTTTGCGCTCAAACGCCGCACCTAATTc                                                                                                                                                                                    >  1:254529/1‑147 (MQ=255)                                                                                                                                                                        ggTGAAATCAATCAGTAAATCGAAACGATCTTTCACCGCGTTTAAGTCATCGCCGACCTTAATGCCTAAGTTGCCGATCCCCGCCAATTCGCCGGCATCGGCGCCCACCAATGAGGAACCTTTGCGCTCAAACGCCGCACCTAATTCCACGCCTTCCGCGTTATGTACCGCCTGAATTAATTGGCGACCCATACGTCCGCCTGCGCCCACTACAGCAATTTTTAATGTCATATTGTTTCTCCCGAaaataa                                                                            >  1:890670/1‑251 (MQ=255)                                                                                                                                                                                    caGTAAATCGAAACGATCTTTCACCGCGTTTAAGTCATCGCCGACCTTAATGCCTAAGTTGCCGATCCCCGCCAATTCGCCGGCATCGGCGCCCACCAATGAGGAACCTTTGCGCTCAAACGCCGCACCTAATTCCACGCCTTCCGCGTTATGTACCGCCTGAATTAATTGGCGACCCATACGTCCGCCTGCGCCCACTAc                                                                                                                  >  1:323654/1‑201 (MQ=255)                                                                                                                                                                                       tAAATCGAAACGATCTTTCACCGCGTTTAAGTCATCGCCGACCTTAATGCCTAAGTTGCCGATCCCCGCCAATTCGCCGGCATCGGCGCCCACCAATGAGGAACCTTTGCGCTCAAACGCCGCACCTAATTCCACGCCTTCCGCGTTATGTACCGCCTGAATTAATTGGCGACCCATACGTCCGCCTGCGCCCACTACAGCAATTTTTAATGTCATATTGTTTCTCCCGAAAATAAATTAGCCTAacac                                                               <  1:821677/249‑1 (MQ=255)                                                                                                                                                                                       tAAATCGAAACGATCTTTCACCGCGTTTAAGTCATCGCCGACCTTAATGCCTAAGTTGCCGATCCCCGCCAATTCGCCGGCATCGGCGCCCACCAATGAGGAACCTTTGCGCTCAAACGCCGCACCTAATTCCACGCCTTCCGCGTTATGTACCGCCTGAATTAATTGGCGACCCATACGTCCGCCTGCGCCCACTACAGCAATTTTTAATGTCATATTGTTTCTCCCGAAAATAAATTAGCCTAacac                                                               <  1:851356/249‑1 (MQ=255)                                                                                                                                                                                         aaTCGAAACGATCTTTCACCGCGTTTAAGTCATCGCCGACCTTAATGCCTAAGTTGCCGATCCCCGCCAATTCGCCGGCATCGGCGCCCACCAATGAGGAACCTTTGCGCTCAAACGCCGCACCTAATTCCACGCCTTCCGCGTTATGTACCGCCTGAATTAATTGGCGACCCATACGTCCGCCTGCGCCCACTACAGCAATTTTTAATGTCATATTGTTTCTCCCGAAAATAAATTAGCCTAACACTTTc                                                           <  1:893513/251‑1 (MQ=255)                                                                                                                                                                                                   aTCTTTCACCGCGTTTAAGTCATCGCCGACCTTAATGCCTAAGTTGCCGATCCCCGCCAATTCGCCGGCATCGGCGCCCACCAATGAGGAACCTTTGCGCTCAAACGCCGCACCTAATTCCACGCCTTCCGCGTTATGTACCGCCTGaattaatt                                                                                                                                                 >  1:129456/1‑155 (MQ=255)                                                                                                                                                                                                   aTCTTTCACCGCGTTTAAGTCATCGCCGACCTTAATGCCTAAGTTGCCGATCCCCGCCAATTCGCCGGCATCGGCGCCCACCAATGAGGAACCTTTGCGCTCAAACGCCGCACCTAATTCCACGCCTTCCGCGTTATGTACCGCCTGAATTAATTGGCGACCCATACGTCCGCCTGCGCCCACTAc                                                                                                                  <  1:605725/186‑1 (MQ=255)                                                                                                                                                                                                   aTCTTTCACCGCGTTTAAGTCATCGCCGACCTTAATGCCTAAGTTGCCGATCCCCGCCAATTCGCCGGCATCGGCGCCCACCAATGAGGAACCTTTGCGCTCAAACGCCGCACCTAATTCCACGCCTTCCGCGTTATGTACCGCCTGAATTAATTGGCGACCCATACGTCCGCCTGCGCCCACTACAGCAATTTTTAATGTCATATTGTTTCTCCCGAAAATAAATTAGcc                                                                     >  1:850415/1‑231 (MQ=255)                                                                                                                                                                                                   aTCTTTCACCGCGTTTAAGTCATCGCCGACCTTAATGCCTAAGTTGCCGATCCCCGCCAATTCGCCGGCATCGGCGCCCACCAATGAGGAACCTTTGCGCTCAAACGCCGCACCTAATTCCACGCCTTCCGCGTTATGTACCGCCTGAATTAATTGGCGACCCATACGTCCGCCTGCGCCCACTACAGCAATTTTTAATGTCATATTGTTTCTCCCGAAAATAAATTAGCCTAacac                                                               <  1:745333/237‑1 (MQ=255)                                                                                                                                                                                                   aTCTTTCACCGCGTTTAAGTCATCGCCGACCTTAATGCCTAAGTTGCCGATCCCCGCCAATTCGCCGGCATCGGCGCCCACCAATGAGGAACCTTTGCGCTCAAACGCCGCACCTAATTCCACGCCTTCCGCGTTATGTACCGCCTGAATTAATTGGCGACCCATACGTCCGCCTGCGCCCACTACAGCAATTTTTAATGTCATATTGTTTCTCCCGAAAATAAATTAGCCTAACACTTTCAGCGcacca                                                  >  1:132176/1‑250 (MQ=255)                                                                                                                                                                                                   aTCTTTCACCGCGTTTAAGTCATCGCCGACCTTAATGCCTAAGTTGCCGATCCCCGCCAATTCGCCGGCATCGGCGCCCACCAATGAGGAACCTTTGCGCTCAAACGCCGCACCTAATTCCACGCCTTCCGCGTTATGTACCGCCTGAATTAATTGGCGACCCATACGTCCGCCTGCGCCCACTACAGCAATTTTTAATGTCATATTGTTTCTCCCGAAAATAAATTAGCCTAACACTTTCAGCGcacca                                                  >  1:892156/1‑250 (MQ=255)                                                                                                                                                                                                         cACCGCGTTTAAGTCATCGCCGACCTTAATGCCTAAGTTGCCGATCCCCGCCAATTCGCCGGCATCGGCGCCCACCAATGAGGAACCTTTGCGCTCAAACGCCGCACCTAATTCCACGCCTTCCGCGTTATGTACCGCCTGAATTAATTGGCGACCCATACGTCCGCCTGCGCCCACTACAGCAATTTTTAATGTCATATTGTTTCTCCCGAAAATAAATTAGCCTAACACTTTCAGCGCACCACTATaaa                                           <  1:339623/251‑1 (MQ=255)                                                                                                                                                                                                          cccGCGTTTAAGTCATCGCCGACCTTAATGCCTAAGTTGCCGATCCCCGCCAATTCGCCGGCATCGGCGCCCACCAATGAGGAACCTTTGCGCTCAAACGCCGCACCTAATTCCACGCCTTCCGCGTTATGTACCGCCTGAATTAATTGGCGACCCATACGTCCGCCTGCGCCCACTACAGCAATTTTTAATGTCATATTGTTTCTCCCGAAAATAAATTAGCCTAACACTTTCAGCGCACCACTATAAAt                                          <  1:395866/250‑1 (MQ=255)                                                                                                                                                                                                           ccGCGTTTAAGTCATCGCCGACCTTAATGCCTAAGTTGCCGATCCCCGCCAATTCGCCGGCATCGGCGCCCACCAATGAGGAACCTTTGCGCTCAAACGCCGCACCTAATTCCACGCCTTCCGCGTTATGTACCGCCTGAATTAATTGGCGACCCATACGTCCGCCTGCGCCCACTACAGCAATTTTTAATGTCATATTGTTTCTCCCGAAAATAAATTAGCCTAACACTTTCAGCGCACCACTATAAAtt                                         <  1:187490/251‑1 (MQ=255)                                                                                                                                                                                                             gcgTTTAAGTCATCGCCGACCTTAATGCCTAAGTTGCCGATCCCCGCCAATTCGCCGGCATCGGCGCCCACCAATGAGGAACCTTTGCGCTCAAACGCCGCACCTAATTCCACGCCTTCCGCGTTATGTACCGcc                                                                                                                                                           >  1:872302/1‑135 (MQ=255)                                                                                                                                                                                                             gcgTTTAAGTCATCGCCGACCTTAATGCCTAAGTTGCCGATCCCCGCCAATTCGCCGGCATCGGCGCCCACCAATGAGGAACCTTTGCGCTCAAACGCCGCACCTAATTCCACGCCTTCCGCGTTATGTACCGCCTGAATTAATTGGCGACCCATACGTCCGCCTGCGCCCACTACAGCAATTTTTAATGTCATATTGTTTCTCCCGAAAATAAATTAGCCTAacac                                                               <  1:324429/227‑1 (MQ=255)                                                                                                                                                                                                             gcgTTTAAGTCATCGCCGACCTTAATGCCTAAGTTGCCGATCCCCGCCAATTCGCCGGCATCGGCGCCCACCAATGAGGAACCTTTGCGCTCAAACGCCGCACCTAATTCCACGCCTTCCGCGTTATGTACCGCCTGAATTAATTGGCGACCCATACGTCCGCCTGCGCCCACTACAGCAATTTTTAATGTCATATTGTTTCTCCCGAAAATAAATTAGCCTAACACTTTCAGCGCACCACTATAAATTaa                                       >  1:195103/1‑251 (MQ=255)                                                                                                                                                                                                                tttAAGTCATCGCCGACCTTAATGCCTAAGTTGCCGATCCCCGCCAATTCGCCGGCATCGGCGCCCACCAATGAGGAACCTTTGCGCTCAAACGCCGCACCTAATTCCACGCCTTCCGCGTTATGTACCGCCTGAATTAATTGGCGACCCATACGTCCGCCTGCGCCCACTACAGCAATTTTTAATGTCATATTGTTTCTCCCGAAAATAAATTAGCCTAACACTTTCAGCGCACCACTATAAATTAAGt                                     <  1:276108/250‑1 (MQ=255)                                                                                                                                                                                                                 ttAAGTCATCGCCGACCTTAATGCCTAAGTTGCCGATCCCCGCCAATTCGCCGGCATCGGCGCCCACCAATGAGGAACCTTTGCGCTCAAACGCCGCACCTAATTCCACGCCTTCCGCGTTATGTACCGCCTGAATTAATTGGCGACCCATACGTCCGCCTGCGCCCACTACAGCAATTTTTAATGTCATATTGTTTCTCCCGAAAATAAATTAGCCTAACACTTTCAGCGCACCACTATAAATTAAGTAg                                   <  1:319372/251‑1 (MQ=255)                                                                                                                                                                                                                    aGTCATCGCCGACCTTAATGCCTAAGTTGCCGATCCCCGCCAATTCGCCGGCATCGGCGCCCACCAATGAGGAACCTTTGCGCTCAAACGCCGCACCTAATTCCACGCCTTCCGCGTTATGTACCGCCTGAATTAATTGGCGACCCATACGTCCGCCTGCGCCCACTACAGCAATTTTTAATGTCATATTGTTTCTCCCGAAAATAAATTAGCCTAACACTTTCAGCGCACCACTATaa                                            >  1:13278/1‑239 (MQ=255)                                                                                                                                                                                                                    aGTCATCGCCGACCTTAATGCCTAAGTTGCCGATCCCCGCCAATTCGCCGGCATCGGCGCCCACCAATGAGGAACCTTTGCGCTCAAACGCCGCACCTAATTCCACGCCTTCCGCGTTATGTACCGCCTGAATTAATTGGCGACCCATACGTCCGCCTGCGCCCACTACAGCAATTTTTAATGTCATATTGTTTCTCCCGAAAATAAATTAGCCTAACACTTTCAGCGCACCACTATAAATTAAGTAGATg                                >  1:19327/1‑251 (MQ=255)                                                                                                                                                                                                                    aGTCATCGCCGACCTTAATGCCTAAGTTGCCGATCCCCGCCAATTCGCCGGCATCGGCGCCCACCAATGAGGAACCTTTGCGCTCAAACGCCGCACCTAATTCCACGCCTTCCGCGTTATGTACCGCCTGAATTAATTGGCGACCCATACGTCCGCCTGCGCCCACTACAGCAATTTTTAATGTCATATTGTTTCTCCCGAAAATAAATTAGCCTAACACTTTCAGCGCACCACTATAAATTAAGTAGATg                                >  1:379675/1‑251 (MQ=255)                                                                                                                                                                                                                     gTCATCGCCGACCTTAATGCCTAAGTTGCCGATCCCCGCCAATTCGCCGGCATCGGCGCCCACCAATGAGGAACCTTTGCGCTCAAACGCCGCACCTAATTCCACGCCTTCCGCGTTATGTACCGCCTGAATTAATTGGCGACCCATACGTCCGCCTGCGCCCACTACAGCAATTTTTAATGTCATATTGTTTCTCCCGAAAATAAATTAGCCTAACACTTTCAgcgc                                                      >  1:626917/1‑228 (MQ=255)                                                                                                                                                                                                                               aCCTTAATGCCTAAGTTGCCGATCCCCGCCAATTCGCCGGCATCGGCGCCCACCAATGAGGAACCTTTGCGCTCAAACGCCGCACCTAATTCCACGCCTTCCGCGTTATGTACCGCCTGAATTAATTGGCGACCCATACGTCCGCCTGCGCCCACTACAGCAATTTTTAATGTCATATTGTTTCTCCCGAAAATAAATTAGCCTAACACTTTCAGCGCACCGCTATAAATTAAGTAGATGCCGaataaaat                     <  1:98444/251‑1 (MQ=255)                                                                                                                                                                                                                                ccTTAATGCCTAAGTTGCCGATCCCCGCCAATTCGCCGGCATCGGCGCCCACCAATGAGGAACCTTTGCGCTCAAACGCCGCACCTAATTCCACGCCTTCCGCGTTATGTACCGCCTGAATTAATTGGCGACCCATACGTCCGCCTGCGCCCACTACAGCAATTTTTAATGTCATATTGTTTCTCCCGAAAATAAATTAGCCTAACACTTTCAGCGCACCACTATAAATTAAGTAGATGCCGaataaaata                    >  1:384148/1‑251 (MQ=255)                                                                                                                                                                                                                                ccTTAATGCCTAAGTTGCCGATCCCCGCCAATTCGCCGGCATCGGCGCCCACCAATGAGGAACCTTTGCGCTCAAACGCCGCACCTAATTCCACGCCTTCCGCGTTATGTACCGCCTGAATTAATTGGCGACCCATACGTCCGCCTGCGCCCACTACAGCAATTTTTAATGTCATATTGTTTCTCCCGAAAATAAATTAGCCTAACACTTTCAGCGCACCACTATAAATTAAGTAGATGCCGaataaaata                    >  1:459858/1‑251 (MQ=255)                                                                                                                                                                                                                                 cTTAATGCCTAAGTTGCCGATCCCCGCCAATTCGCCGGCATCGGCGCCCACCAATGAGGAACCTTTGCGCTCAAACGCCGCACCTAATTCCACGCCTTCCGCGTTATGTACCGCCTGAATTAATTGGCGAcc                                                                                                                                          >  1:387641/1‑132 (MQ=255)                                                                                                                                                                                                                                               tGCCGATCCCCGCCAATTCGCCGGCATCGGCGCCCACCAATGAGGAACCTTTGCGCTCAAACGCCGCACCTAATTCCACGCCTTCCGCGTTATGTACCGCCTGAATTAATTGGCGACCCATACGTCCGCCTGCGCCCACTACAGCAATTTTTAATGTCATATTGTTTCTCCCGAAAATAAATTAGCCTAACACTTTCAGCGCACCACTATAAATTAAGTAGATGCCGAATAAAATAAAAATTACGCCCGaa     >  1:421816/1‑251 (MQ=255)                                                                                                                                                                                                                                                gCCGATCCCCGCCAATTCGCCGGCATCGGCGCCCACCAATGAGGAACCTTTGCGCTCAAACGCCGCACCTAATTCCACGCCTTCCGCGTTATGTACCGCCTGAATTAATTGGCGACCCATACGTCCGCCTGCGCCCACTACAGCAATTTTTAATGTCATATTGTTTCTCCCGAAAATAAATTAGCCTAACACTTTCAGCGCACCACTATaa                                            >  1:386178/1‑211 (MQ=255)                                                                                                                                                                                                                                                  cGATCCCCGCCAATTCGCCGGCATCGGCGCCCACCAATGAGGAACCTTTGCGCTCAAACGCCGCACCTAATTCCACGCCTTCCGCGTTATGTACCGCCTGAATTAATTGGCGACCCATACGTCCGCCTGCGCCCACTACAGCAATTTTTAATGTCATATTGTTTCTCCCGAAAATAAATTAGCCTAACACTTTCAGCGCACCACTATAAATTAAGTAGATGCCGAATAAAATAAAAATTACGCCCGAAACg  <  1:475936/251‑1 (MQ=255)                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                       CCAAATTCACGCCGACACTGAAATTTGAAGCAAACACGATAGCGGTCTTTTCGGCTGCTGATTTAATTGCCGCTTTGCCGGCGTCATCAAAACCCGTGGTGCCGATAATGATTTTTTTATGGTTGGCAACACAAAATGCCAAATGTTCCAATGTGCCTT‑CCGGGCGGGTGAAATCAATCAGTAAATCGAAACGATCTTTCACCGCGTTTAAGTCATCGCCGACCTTAATGCCTAAGTTGCCGATTCCCGCCAATTCGCCGGCATCGGCGCCCACCAATGAGGAACCTTTGCGCTCAAACGCCGCACCTAATTCCACGCCTTCCGCGTTATGTACCGCCTGAATTAATTGGCGACCCATACGTCCGCCTGCGCCCACTACAGCAATTTTTAATGTCATATTGTTTCTCCCGAAAATAAATTAGCCTAACACTTTCAGCGCACCACTATAAATTAAGTAGATGCCGAATAAAATAAAAATTACGCCCGAAACG  >  1/1616474‑1616964

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑