Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,616,582	T→C	intergenic (‑171954/‑25182)	unknown ← / → unknown	tRNA‑Met‑CAT/tRNA‑Gly‑GCC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,616,582	0	T	C	100.0%	216.4 / NA	78	intergenic (‑171954/‑25182)	unknown/unknown	tRNA‑Met‑CAT/tRNA‑Gly‑GCC
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (36/42);  total (36/42)

GGTTTTGGCAATATGTTCGCCCATGGAAAGCGCGGTACCGGACGGCGCATCGACTTTATAGCGGTGGTGCGCTTCGATGATTTCGATGTCGCAATAATCGCCCATCACCTTGGCGGCTTTTTCCAATAATTTAAAGACCAAATTCACGCCGACACTGAAATTTGAAGCAAACACGATAGCGGTCTTTTCGGCTGCTGATTTAATTGCCGCTTTGCCGGCGTCATCAAAACCCGTGGTGCCGATAATGATTTTTTTATGGTTGGCAACACAAAATGCCAAATGTTCCAATGTGCCTT‑CCGGGCGGGTGAAATCAATCAGTAAATCGAAACGATCTTTCACCGCGTTTAAGTCATCGCCGACCTTAATGCCTAAGTTGCCGATTCCCGCCAATTCGCCGGCATCGGCGCCCACCAATGAGGAACCTTTGCGCTCAAACGCCGCACCTAATTCCACGCCTTCCGCGTTATGTACCGCC  >  1/1616337‑1616811                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                       ggTTTTGGCAATATGTTCGCCCATGGAAAGCGCGGTACCGGACGGCGCATCGACTTTATAGCGGTGGTGCGCTTCGATGATTTCGATGTCGCAATAATCGCCCATCACCTTGGCGGCTTTTTCCAATAATTTAAAGACCAAATTCACGCCGACACTGAAATTTGAAGCAAACACGATAGCGGTCTTTTCGGCTGCCGATTTAATTGCCGCTTTGCCGGCGTCATCAAAACCCGTGGTGCCGATAACGAttt                                                                                                                                                                                                                                   >  1:86861/1‑251 (MQ=255)      tGGCAATATGTTCGCCCATGGAAAGCGCGGTACCGGACGGCGCATCGACTTTAAAGCGGTGGTGCGCTTCGATGATTTCGATGTCGCAATAATCGCCCATCACCTTGGCGGCTTTTTCCAATAATTTAAAGACCAAATTCACGCCGACACTGAAATTTGAAGCAAACACGATAGCGGTCTTTTCGGCTGCCGATTTAATTGCCGCTTTGCCGGCGTCATCAAAACCCGTGGTGCCGATAACGATTTTTTTa                                                                                                                                                                                                                              <  1:317497/251‑1 (MQ=255)         cAATATGTTCGCCCATGGAAAGCGCGGTACCGGACGGCGCATCGACTTTATAGCGGTGGTGCGCTTCGATGATTTCGATGTCGCAATAATCGCCCATCACCTTGGCGGCTTTTTCCAATAATTTAAAGACCAAATTCACGCCGACACTGAAATTTGAAGCAAACACGATAGCGGTCTTTTCGGCTGCCGATTTAATTGCCGCTTTGCCGGCGTCATCAAAACCCGTGGTGCCGATAACGATTTTTTTATgg                                                                                                                                                                                                                           <  1:482563/251‑1 (MQ=255)                    cccATGGAAAGCGCGGTACCGGACGGCGCATCGACTTTATAGCGGTGGTGCGCTTCGATGATTTCGATGTCGCAATAATCGCCCATCACCTTGGCGGCTTTTTCCAATAATTTAAAGACCAAATTCACGCCGACACTGAAATTTGAAGCAAACACGATAGCGGTCTTTTCGGCTGCCGATTTAATTGCCGCTTTGCCGGCGTCATCAAAACCCGTGGTGCCGATAACGATTTTTTTATGGTTGGCAacac                                                                                                                                                                                                                 <  1:202219/250‑1 (MQ=255)                    cccATGGAAAGCGCGGTACCGGACGGCGCATCGACTTTATAGCGGTGGTGCGCTTCGATGATTTCGATGTCGCAATAATCGCCCATCACCTTGGCGGCTTTTTCCAATAATTTAAAGACCAAATTCACGCCGACACTGAAATTTGAAGCAAACACGATAGCGGTCTTTTCGGCTGCCGATTTAATTGCCGCTTTGCCGGCGTCATCAAAACCCGTGGTGCCGATAACGATTTTTTTATGGTTGGCAAcaca                                                                                                                                                                                                                >  1:470221/1‑251 (MQ=255)                    cccATGGAAAGCGCGGTACCGGACGGCGCATCGACTTTATAGCGGTGGTGCGCTTCGATGATTTCGATGTCGCAATAATCGCCCATCACCTTGGCGGCTTTTTCCAATAATTTAAAGACCAAATTCACGCCGACACTGAAATTTGAAGCAAACACGATAGCGGTCTTTTCGGCTGCCGATTTAATTGCCGCTTTGCCGGCGTCATCAAAACCCGTGGTGCCGATAACGATTTTTTTATGGTTGGCAAcaca                                                                                                                                                                                                                >  1:316873/1‑251 (MQ=255)                    cccATGGAAAGCGCGGTACCGGACGGCGCATCGACTTTATAGCGGTGGTGCGCTTCGATGATTTCGATGTCGCAATAATCGCCCATCACCTTGGCGGCTTTTTCCAATAATTTAAAGACCAAATTCACGCCGACACTGAAATTTGAAGCAAACACGATAGCGGTCTTTTCGGCTGCCGATTTAATTGCCGCTTTGCCGGCGTCATCAAAACCCGTGGTGCCGATAACGATTTTTTTATGGTTGGCAAcaca                                                                                                                                                                                                                >  1:499402/1‑251 (MQ=255)                              gcgcGGTACCGGACGGCGCATCGACTTTATAGCGGTGGTGCGCTTCGATGATTTCGATGTCGCAATAATCGCCCATCACCTTGGCGGCTTTTTCCAATAATTTAAAGACCAAATTCACGCCGACACTGAAATTTGAAGCAAACACGATAGCGGTCTTTTCGGCTGCCGATTTAATTGCCGCTTTGCCGGCGTCATCAAAACCCGTGGTGCCGATAACGATTTTTTTATGGTTGGCAACACAAAATGCCaaa                                                                                                                                                                                                      >  1:117717/1‑251 (MQ=255)                                   gTACCGGACGGCGCATCGACTTTATAGCGGTGGTGCGCTTCGATGATTTCGATGTCGCAATAATCGCCCATCACCTTGGCGGCTTTTTCCAATAATTTAAAGACCAAATTCACGCCGACACTGAAATTTGAAGCAAACACGATAGCGGTCTTTTCGGCTGCCGATTTAATTGCCGCTTTGCCGGCGTCATCAAAACCCGTGGTGCCGATAACGATTTTTTTATgg                                                                                                                                                                                                                           >  1:886353/1‑225 (MQ=255)                                   gTACCGGACGGCGCATCGACTTTATAGCGGTGGTGCGCTTCGATGATTTCGATGTCGCAATAATCGCCCATCACCTTGGCGGCTTTTTCCAATAATTTAAAGACCAAATTCACGCCGACACTGAAATTTGAAGCAAACACGATAGCGGTCTTTTCGGCTGCCGATTTAATTGCCGCTTTGCCGGCGTCATCAAAACCCGTGGTGCCGATAACGATTTTTTTATGGTTGGCAACACAAAATGCCAAATGtt                                                                                                                                                                                                  >  1:640287/1‑250 (MQ=255)                                   gTACCGGACGGCGCATCGACTTTATAGCGGTGGTGCGCTTCGATGATTTCGATGTCGCAATAATCGCCCATCACCTTGGCGGCTTTTTCCAATAATTTAAAGACCAAATTCACGCCGACACTGAAATTTGAAGCAAACACGATAGCGGTCTTTTCGGCTGCCGATTTAATTGCCGCTTTGCCGGCGTCATCAAAACCCGTGGTGCCGATAACGATTTTTTTATGGTTGGCAACACAAAATGCCAAATGtt                                                                                                                                                                                                  >  1:449103/1‑250 (MQ=255)                                         gACGGCGCATCGACTTTATAGCGGTGGTGCGCTTCGATGATTTCGATGTCGCAATAATCGCCCATCACCTTGGCGGCTTTTTCCAATAATTTAAAGACCAAATTCACGCCGACACTGAAATTTGAAGCAAACACGATAGCGGTCTTTTCGGCTGCCGATTTAATTGCCGCTTTGCCGGCGTCATCAAAACCCGTGGTGCCGATAACGATTTTTTTATGGTTGGCAACACAAAATGCCAAATGTTCCAAtg                                                                                                                                                                                            <  1:209740/250‑1 (MQ=255)                                             gcgcATCGACTTTATAGCGGTGGTGCGCTTCGATGATTTCGATGTCGCAATAATCGCCCATCACCTTGGCGGCTTTTTCCAATAATTTAAAGACCAAATTCACGCCGACACTGAAATTTGAAGCAAACACGATAGCGGTCTTTTCGGCTGCCGATTTAATTGCCGCTTTGCCGGCGTCATCAAAACCCGTGGTGCCGATAACGATTTTTTTATGGTTGGCAACACAAAATg                                                                                                                                                                                                           <  1:132080/231‑1 (MQ=255)                                                     aCTTTATAGCGGTGGTGCGCTTCGATGATTTCGATGTCGCAATAATCGCCCATCACCTTGGCGGCTTTTTCCAATAATTTAAAGACCAAATTCACGCCGACACTGAAATTTGAAGCAAACACGATAGCGGTCTTTTCGGCTGCCGATTTAATTGCCGCTTTGCCGGCGTCATCAAAACCCGTGGTGCCGATAACGAt                                                                                                                                                                                                                                     <  1:23605/197‑1 (MQ=255)                                                     aCTTTATAGCGGTGGTGCGCTTCGATGATTTCGATGTCGCAATAATCGCCCATCACCTTGGCGGCTTTTTCCAATAATTTAAAGACCAAATTCACGCCGACACTGAAATTTGAAGCAAACACGATAGCGGTCTTTTCGGCTGCCGATTTAATTGCCGCTTTGCCGGCGTCATCAAAACCCGTGGTGCCGATAACGATTTTTTTATgg                                                                                                                                                                                                                           >  1:250437/1‑207 (MQ=255)                                                     aCTTTATAGCGGTGGTGCGCTTCGATGATTTCGATGTCGCAATAATCGCCCATCACCTTGGCGGCTTTTTCCAATAATTTAAAGACCAAATTCACGCCGACACTGAAATTTGAAGCAAACACGATAGCGGTCTTTTCGGCTGCCGATTTAATTGCCGCTTTGCCGGCGTCATCAAAACCCGTGGTGCCGATAACGATTTTTTTATGGTTGGCAACACAAAATGCCAAATGTTCCAATGTGCCTT‑Ccgggcg                                                                                                                                                                              >  1:248903/1‑251 (MQ=255)                                                      cTTTATAGCGGTGGTGCGCTTCGATGATTTCGATGTCGCAATAATCGCCCATCACCTTGGCGGCTTTTTCCAATAATTTAAAGACCAAATTCACGCCGACACTGAAATTTGAAGCAAACACGATAGCGGTCTTTTCGGCTGCCGATTTAATTGCCGCTTTGCCGGCGTCATCAAAACCCGTGGTGCCGATAACGATTTTTTTATGGTTGGCAACACAAAATGCCAAATGTTCCAATGTGCCTT‑Ccgggcgg                                                                                                                                                                             >  1:212593/1‑251 (MQ=255)                                                        ttATAGCGGTGGTGCGCTTCGATGATTTCGATGTCGCAATAATCGCCCATCACCTTGGCGGCTTTTTCCAATAATTTAAAGACCAAATTCACGCCGACACTGAAATTTGAAGCAAACACGATAGCGGTCTTTTCGGCTGCCGATTTAATTGCCGCTTTGCCGGCGTCATCAAAACCCGTGGTGCCGATAACGATTTTTTTATGGTTGGCAACACAAAATGCCAAATGTTCCAATGTGCCTT‑CCGGGCGGGt                                                                                                                                                                           >  1:806613/1‑251 (MQ=255)                                                             gCGGTGGTGCGCTTCGATGATTTCGATGTCGCAATAATCGCCCATCACCTTGGCGGCTTTTTCCAATAATTTAAAGACCAAATTCACGCCGACACTGAAATTTGAAGCAAACACGATAGCGGTCTTTTCGGCTGCCGATTTAATTGCCGCTTTGCCGGCGTCATCAAAACCCGTGGTGCCGATAACGATTTTTTTATGGTTGGCAACACAAAATGCCAAATGTTCCAATGTGCCTT‑CCGGGCGGGTGAaat                                                                                                                                                                      >  1:564040/1‑251 (MQ=255)                                                              cGGTGGTGCGCTTCGATGATTTCGATGTCGCAATAATCGCCCATCACCTTGGCGGCTTTTTCCAATAATTTAAAGACCAAATTCACGCCGACACTGAAGTTTGAAGCAAACACGATAGCGGTCTTTTCGGCTGCCGATTTAATTGCCGCTTTGCCGGCGTCATCAAAACCCGTGGTGCCGATAACGATTTTTTTATGGTTGGCAACACAAAATGCCAAATGTTCCAATGTGCCTT‑CCGGGCGGGTGAaatc                                                                                                                                                                     <  1:564329/251‑1 (MQ=255)                                                                gtggtgCGCTTCGATGATTTCGATGTCGCAATAATCGCCCATCACCTTGGCGGCTTTTTCCAATAATTTAAAGACCAAATTCACGCCGACACTGAAATTTGAAGCAAACACGATAGCGGTCTTTTCGGCTGCCGATTTAATTGCCGCTTTGCCGGCGTCATCAAAACCCGTGGTGCCGATAACGATTTTTTTATGGTTGGCAACACAAAATGc                                                                                                                                                                                                          >  1:283933/1‑213 (MQ=255)                                                                gtggtgCGCTTCGATGATTTCGATGTCGCAATAATCGCCCATCACCTTGGCGGCTTTTTCCAATAATTTAAAGACCAAATTCACGCCGACACTGAAATTTGAAGCAAACACGATAGCGGTCTTTTCGGCTGCCGATTTAATTACCGCTTTGCCGGCGTCATCAAAACCCGTGGTGCCGATAACGATTTTTTTATGGTTGGCAACACAAAATGCCAAATGTTCCAATGTGCCTT‑CCGGGCGGGTGAaatcaa                                                                                                                                                                   >  1:83183/1‑251 (MQ=255)                                                                                atTTCGATGTCGCAATAATCGCCCATCACCTTGGCGGCTTTTTCCAATAATTTAAAGACCAAATTCACGCCGACACTGAAATTTGAAGCAAACACGATAGCGGTCTTTTCGGCTGCCGATTTAATTGCCGCTTTGCCGGCGTCATCAAAACCCGTGGTGCCGATAACGATTTTTTTATGGTTGGCAACACAAAATGCCAAATGTTCCAATGTGCCTT‑CCGGGCGGGTGAAATCAATCAGTAAATCGAAAc                                                                                                                                                    <  1:727523/250‑1 (MQ=255)                                                                                       tGTCGCAATAATCGCCCATCACCTTGGCGGCTTTTTCCAATAATTTAAAGACCAAATTCACGCCGACACTGAAATTTGAAGCAAACACGATAGCGGTCTTTTCGGCTGCCGATTTAATTGCCGCTTTGCCGGCGTCATCAAAACCCGTGGTGCCGATAACGATTTTTTTATGGTTGGCAACACAAAATGCCAAATGTTCCAATGTGCCTT‑CCGGGCGGGTGAAATCAATCAGTAAATCGAAACGATCTTTc                                                                                                                                            <  1:479651/251‑1 (MQ=255)                                                                                        gTCGCAATAATCGCCCATCACCTTGGCGGCTTTTTCCAATAATTTAAAGACCAAATTCACGCCGACACTGAAATTTGAAGCAAACACGATAGCGGTCTTTTCGGCTGCCGATTTAATTGCCGCTTTGCCGGCGTCATCAAAACCCGTGGTGCCGATAACGATTTTTTTATGGTTGGCAACACAAAATGCCAAATGTTCCAATGTGCCTT‑CCGGGCGGGTGAAATCAATCAGTAAATCGAAACGATCTTTCa                                                                                                                                           >  1:539429/1‑251 (MQ=255)                                                                                         tCGCAATAATCGCCCATCACCTTGGCGGCTTTTTCCAATAATTTAAAGACCAAATTCACGCCGACACTGAAATTTGAAGCAAACACGATAGCGGTCTTTTCGGCTGCCGATTTAATTGCCGCTTTGCCGGCGTCATCAAAACCCGTGGTGCCGATAACGATTTTTTTATGGTTGGCAACACAAAATGCCAAATGTTCCAATGTGCCTT‑CCGGGCGGGTGAAATCAATCAGTAAATCGAAACGATCTTTCAc                                                                                                                                          <  1:602439/251‑1 (MQ=255)                                                                                         tCGCAATAATCGCCCATCACCTTGGCGGCTTTTTCCAATAATTTAAAGACCAAATTCACGCCGACACTGAAATTTGAAGCAAACACGATAGCGGTCTTTTCGGCTGCCGATTTAATTGCCGCTTTGCCGGCGTCATCAAAACCCGTGGTGCCGATAACGATTTTTTTATGGTTGGCAACACAAAATGCCAAATGTTCCAATGTGCCTT‑CCGGGCGGGTGAAATCAATCAGTAAATCGAAACGATCTTTCAc                                                                                                                                          <  1:600100/251‑1 (MQ=255)                                                                                         tCGCAATAATCGCCCATCACCTTGGCGGCTTTTTCCAATAATTTAAAGACCAAATTCACGCCGACACTGAAATTTGAAGCAAACACGATAGCGGTCTTTTCGGCTGCCGATTTAATTGCCGCTTTGCCGGCGTCATCAAAACCCGTGGTGCCGATAACGATTTTTTTATGGTTGGCAACACAAAATGCCAAATGTTCCAATGTGCCTT‑CCGGGCGGGTGAAATCAATCAGTAAATCGAAACGATCTTTCAc                                                                                                                                          <  1:158407/251‑1 (MQ=255)                                                                                         tCGCAATAATCGCCCATCACCTTGGCGGCTTTTTCCAATAATTTAAAGACCAAATTCACGCCGACACTGAAATTTGAAGCAAACACGATAGCGGTCTTTTCGGCTGCCGATTTAATTGCCGCTTTGCCGGCGTCATCAAAACCCGTGGTGCCGATAACGATTTTTTTATGGTTGGCAACACAAAATGCCAAATGTTCCAATGTGCCTT‑CCGGGCGGGTGAAATCAATCAGTAAATCGAAACGATCTTTCAc                                                                                                                                          <  1:104952/251‑1 (MQ=255)                                                                                               taatCGCCCATCACCTTGGCGGCTTTTTCCAATAATTTAAAGACCAAATTCACGCCGACACTGAAATTTGAAGCAAACACGATAGCGGTCTTTTCGGCTGCCGATTTAATTGCCGCTTTGCCGGCGTCATCAAAACCCGTGGTGCCGATAACGATTTTTTTATGGTTGGCAACACAAAATGCCAAATGTTCCAATGTGCCTT‑CCGGGCGGGTGAAATCAATCAGTAAATCGAAACGATCTTTCAc                                                                                                                                          <  1:399135/245‑1 (MQ=255)                                                                                                 atCGCCCATCACCTTGGCGGCTTTTTCCAATAATTTAAAGACCAAATTCACGCCGACACTGAAATTTGAAGCAAACACGATAGCGGTCTTTTCGGCTGCCGATTTAATTGCCGCTTTGCCGGCGTCATCAAAACCCGTGGTGCCGATAACGATTTTTTTATGGTTGGCAACACAAAATGCCAAATGTTCCAATGTGCCTT‑CCGGGCGGGTGAAATCAATCAGTAAATCGAAACGATCTTTCACCGCGTTTa                                                                                                                                  >  1:778852/1‑251 (MQ=255)                                                                                                   cGCCCATCACCTTGGCGGCTTTTTCCAATAATTTAAAGACCAAATTCACGCCGACACTGAAATTTGAAGCAAACACGATAGCGGTCTTTTCGGCTGCCGATTTAATTGCCGCTTTGCCGGCGTCATCAAAACCCGTGGTGCCGATAACGATTTTTTTATGGTTGGCAACACAAAATGCCAAATGTTCCAATGTGCCTT‑CCGGGCGGGTGAAATCAATCAGTAAATCGAAACGATCTTTCACCGCGTTTAAg                                                                                                                                <  1:179860/251‑1 (MQ=255)                                                                                                    gCCCATCACCTTGGCGGCTTTTTCCAATAATTTAAAGACCAAATTCACGCCGACACTGAAATTTGAAGCAAACACGATAGCGGTCTTTTCGGCTGCCGATTTAATTGCCGCTTTGCCGGCGTCATCAAAACCCGTGGTGCCGATAACGATTTTTTTATGGTTGGCAACACAAAATGCCAAATGTTCCAATGTGCCTT‑CCGGGCGGGTGAAATCAATCAGTAAATCGAAACGATCTTTCACCGCGTTTAAGt                                                                                                                               >  1:768174/1‑251 (MQ=255)                                                                                                     cccATCACCTTGGCGGCTTTTTCCAATAATTTAAAGACCAAATTCACGCCGACACTGAAATTTGAAGCAAACACGATAGCGGTCTTTTCGGCTGCCGATTTAATTGCCGCTTTGCCGGCGTCATCAAAACCCGTGGTGCCGATAACGATTTTTTTATGGTTGGCAACACAAAATGCCAAATGTTCCAATGTGCCTT‑CCGGGCGGGTGAAATCAATCAGTAAATCGAAACGATCTTTCACCGCGTTTAAGt                                                                                                                               <  1:285323/250‑1 (MQ=255)                                                                                                         tCACCTTGGCGGCTTTTTCCAATAATTTAAAGACCAAATTCACGCCGACACTGAAATTTGAAGCAAACACGATAGCGGTCTTTTCGGCTGCCGATTTAATTGCCGCTTTGCCGGCGTCATCAAAACCCGTGGTGCCGATAACGATTTTTTTATGGTTGGCAACACAAAATGCCAAATGTTCCAATGTGCCTT‑CCGGGCGGGTGAaatcaatc                                                                                                                                                                 >  1:11784/1‑212 (MQ=255)                                                                                                            ccTTGGCGGCTTTTTCCAATAATTTAAAGACCAAATTCACGCCGACACTGAAATTTGAAGCAAACACGATAGCGGTCTTTTCGGCTGCCGATTTAATTGCCGCTTTGCCGGCGTCATCAAAACCCGTGGTGCCGATAACGATTTTTTTATGGTTGGCAACACAAAATGCCAAATGTTCCAATGTGCCTT‑CCGGGCGGGTGAAATCAATCAGTAAATCGAAACGATCTTTCACCGCGTTTAAGTCATCGCCg                                                                                                                       >  1:366235/1‑251 (MQ=255)                                                                                                            ccTTGGCGGCTTTTTCCAATAATTTAAAGACCAAATTCACGCCGACACTGAAATTTGAAGCAAACACGATAGCGGTCTTTTCGGCTGCCGATTTAATTGCCGCTTTGCCGGCGTCATCAAAACCCGTGGTGCCGATAACGATTTTTTTATGGTTGGCAACACAAAATGCCAAATGTTCCAATGTGCCTT‑CCGGGCGGGTGAAATCAATCAGTAAATCGAAACGATCTTTCACCGCGTTTAAGTCATCGCCg                                                                                                                       >  1:36933/1‑251 (MQ=255)                                                                                                            ccTTGCCGGCTTTTTCCACTAATTTACAGACCAAATTCACGCCGACACTGCAATTTGAAGCAAACACGACAGCGGTCTTTTCGGCTGCCGATTCACTTGCCGCTTTGCCGGCGTCATCAAAACCCGTGGTGCCGATAACGATTTTTTTATGGTTGGCAACACAAAATGACAAATGTTCCAATGTGCCTT‑CCGGGCGGGTGAaatc                                                                                                                                                                     >  1:872972/1‑205 (MQ=255)                                                                                                               tGGCGGCTTTTTCCAATAATTTAAAGACCAAATTCACGCCGACACTGAAATTTGAAGCAAACACGATAGCGGTCTTTTCGGCTGCCGATTTAATTGCCGCTTTGCCGGCGTCATCAAAACCCGTGGTGCCGATAACGATTTTTTTATGGTTGGCAACACAAAATGCCAAATGTTCCAATGTGCCTT‑CCGGGCGGGTGAAATCAATCAGTAAATCGAAACGATCTTTCAc                                                                                                                                          >  1:147158/1‑229 (MQ=255)                                                                                                                      tttttCCAATAATTTAAAGACCAAATTCACGCCGACACTGAAATTTGAAGCAAACACGATAGCGGTCTTTTCGGCTGCCGATTTAATTGCCGCTTTGCCGGCGTCATCAAAACCCGTGGTGCCGATAACGATTTTTTTATGGTTGGCAACACAAAATGCCAAATGTTCCAATGTGCCTT‑CCGGGCGGGTGAAATCAATCAGTAAATCGAAACGATCTTTCACCGCGTTTAAGTCATCGCCGACCTTAATGc                                                                                                             <  1:105239/251‑1 (MQ=255)                                                                                                                       ttttCCAATAATTTAAAGACCAAATTCACGCCGACACTGAAATTTGAAGCAAACACGATAGCGGTCTTTTCGGCTGCCGATTTAATTGCCGCTTTGCCGGCGTCATCAAAACCCGTGGTGCCGATAACGATTTTTTTATGGTTGGCAACACAAAATGCCAAATGTTCCAATGTGCCTT‑CCGGGCGGGTGAaatc                                                                                                                                                                     <  1:401606/194‑1 (MQ=255)                                                                                                                         ttCCAATAATTTAAAGACCAAATTCACGCCGACACTGAAATTTGAAGCAAACACGATAGCGGTCTTTTCGGCTGCCGATTTAATTGCCGCTTTGCCGGCGTCATCAAAACCCGTGGTGCCGATAACGATTTTTTTATGGTTGGCAACACAAAATGCCAAATGTTCCaa                                                                                                                                                                                              >  1:389628/1‑168 (MQ=255)                                                                                                                                aatTTAAAGACCAAATTCACGCCGACACTGAAATTTGAAGCAAACACGATAGCGGTCTTTTCGGCTGCCGATTTAATTGCCGCTTTGCCGGCGTCATCAAAACCCGTGGTGCCGATAACGATTTTTTTATGGTTGGCAACACAAAATGCCAAATGTTCCAATGTGCCTT‑CCGGGCGGGTGAAATCAATCAGTAAATc                                                                                                                                                         <  1:22859/197‑1 (MQ=255)                                                                                                                                 atTTAAAGACCAAATTCACGCCGACACTGAAATTTGAAGCAAACACGATAGCGGTCTTTTCGGCTGCCGATTTAATTGCCGCTTTGCCGGCGTCATCAAAACCCGTGGTGCCGATAACGATTTTTTTATGGTTGGCAACACAAAATGCCAAATGTTCCAATGTGCCTT‑CCGGGCGGGTGAAATCAATCAGTAAATCGAAACGATCTTTCACCGCGTTTAAGTCATCGCCGACCTTAATGCCTAAGTTGCCg                                                                                                  <  1:577589/251‑1 (MQ=255)                                                                                                                                    tAAAGACCAAATTCACGCCGACGCTGAAATTTGAAGCAAACACGATAGCGGTCTTTTCGGCTGCCGATTTAATTGCCGCTTTGCCGGCGTCATCAAAACCCGTGGTGCCGATAACGATTTTTTTATGGTTGGCAACACAAAATGCCAAATGTTCCAATGTGCCTT‑CCGGGCGGGTGAAATCAATCAGTAAATCGAAACGATCTTTCACCGCGTTTAAGTCATCGCCGACCTTAAGGCCTAAGTTGCCGAtc                                                                                               <  1:282920/251‑2 (MQ=255)                                                                                                                                      aaGACCAAATTCACGCCGACACTGAAATTTGAAGCAAACACGATAGCGGTCTTTTCGGCTGCCGATTTAATTGCCGCTTTGTCGGCGTCATCAAAACCCGTGGTGCCGATAACGATTTTTTTATGGTTGGCAACACAAAATGCCAAATGTTCCAATGTGCCTT‑CCGGGCGGGTGAAATCAATCAGTAAATCGAAACGATCTTTCACCGCGTTTAAGTCATCGCCGACCTTAATGCCTAAGTTGCCGAtccc                                                                                             <  1:443927/251‑4 (MQ=255)                                                                                                                                          ccAAATTCACGCCGACACTGAAATTTGAAGCAAACACGATAGCGGTCTTTTCGGCTGCCGATTTAATTGCCGCTTTGCCGGCGTCATCAAAACCCGTGGTGCCGATAACGATTTTTTTATGGTTGGCAACACAAAATGCCAAATGTTCCAATGTGCCTT‑CCGGGCGGGTGAAATCAATCAGTAAATCGAAACGATCTTTCACCGCGTTTAAGTCATCGCCGACCTTAATGCCTAAGTTGCCGATCCCCGcc                                                                                         <  1:383781/251‑1 (MQ=255)                                                                                                                                                                      aGCAAACACGATAGCGGTCTTTTCGGCTGCCGATTTAATTGCCGCTTTGCCGGCGTCATCAAAACCCGTGGTGCCGATAACGATTTTTTTATGGTTGGCAACACAAAATGCCAAATGTTCCAATGTGCCTT‑CCGGGCGGGTGAAATCAATCAGTAAATCGAAACGATCTTTCACCGCGTTTAAGTCATCGCCGACCTTAATGCCTAAGTTGCCGATCCCCGCCAATTCGCCGGCATCGGCGCCCACCAATg                                                             >  1:152581/1‑251 (MQ=255)                                                                                                                                                                        cAAACACGATAGCGGTCTTTTCGGCTGCCGATTTAATTGCCGCTTTGCCGGCGTCATCAAAACCCGTGGTGCCGATAACGATTTTTTTATGGTTGGCAACACAAAATGCCAAATGTTCCAATGTGCCTT‑CCGGGCGGGTGAGATCAATCAGTAAATCGAAACGATCTTTCACCGCGTTTAAGTCATCGCCGACCTTAATGCCTAAGTTGCCGATCCCCGCCAATTCGCCGGCATCGGCGCCCACCAATGa                                                            <  1:610461/250‑1 (MQ=255)                                                                                                                                                                         aaaCACGATAGCGGTCTTTTCGGCTGCCGATTTAATTGCCGCTTTGCCGGCGTCATCAAAACCCGTGGTGCCGATAACGATTTTTTTATGGTTGGCAACACAAAATGCCAAATGTTCCAATGTGCCTT‑CCGGGCGGGTGAAATCAATCAGTAAATCGAAACGATCTTTCACCGCGTTTAAGTCATCGCCGACCTTAATGCCTAAGTTGCCGATCCCCGCCAATTCGCCGGCATCGGCGCCCACCAATGAgg                                                          >  1:568871/1‑251 (MQ=255)                                                                                                                                                                         aaaCACGATAGCGGTCTTTTCGGCTGCCGATTTAATTGCCGCTTTGCCGGCGTCATCAAAACCCGTGGTGCCGATAACGATTTTTTTATGGTTGGCAACACAAAATGCCAAATGTTCCAATGTGCCTT‑CCGGGCGGGTGAAATCAATCAGTAAATCGAAACGATCTTTCACCGCGTTTAAGTCATCGCCGACCTTAATGCCTAAGTTGCCGATCCCCGCCAATTCGCCGGCATCGGCGCCCACCAATGAgg                                                          >  1:689798/1‑251 (MQ=255)                                                                                                                                                                           acacGATAGCGGTCTTTTCGGCTGCCGATTTAATTGCCGCTTTGCCGGCGTCATCAAAACCCGTGGTGCCGATAACGATTTTTTTATGGTTGGCAACACAAAATGCCAAATGTTCCAATGTGCCTT‑CCGGGCGGGTGAAATCAATCAGTAAATCGAAACGATCTTTCACCGCGTTTAAGTCATCGCCGACCTTAATGCCTAAGTTGCCGATCCCCGCCAATTCGCCGGCATCGGCGCCCACCAATGAgg                                                          <  1:212110/249‑1 (MQ=255)                                                                                                                                                                            cacGATAGCGGTCTTTTCGGCTGCCGATTTAATTGCCGCTTTGCCGGCGTCATCAAAACCCGTGGTGCCGATAACGATTTTTTTATGGTTGGCAACACAAAATGCCAAATGTTCCaa                                                                                                                                                                                              >  1:622377/1‑117 (MQ=255)                                                                                                                                                                            cacGATAGCGGTCTTTTCGGCTGCCGATTTAATTGCCGCTTTGCCGGCGTCATCAAAACCCGTGGTGCCGATAACGATTTTTTTATGGTTGGCAACACAAAATGCCAAATGTTCCAATGTGCCTT‑CCGGGCGGGTGAAATCAATCAGTAAATCGAAACGATCTTTCACCGCGTTTAAGTCATCGCCGACCTTAATGCCTAAGTTGCCGATCCCCGCCAATTCGCCGGCATCGGCGCCCACCAATGAgg                                                          <  1:582097/248‑1 (MQ=255)                                                                                                                                                                            cacGATAGCGGTCTTTTCGGCTGCCGATTTAATTGCCGCTTTGCCGGCATCATCAAAACCCGTGGTGCCGATAACGATTTTTTTACGATTGGTGACACAAAATGCCAAGTGTTCCAATGTGCCTTCCCGGGCGGGTGAAATCAATCAGCAAATCGAAACGATCTTTCGCCGCGTTTAAGTCATCGCCGACCTTAATGCCTAAGTTGCCGATCCCTGCCAATTCGCCGGCATCGGCGCCCACCAATGAGGaa                                                        >  1:66080/1‑251 (MQ=255)                                                                                                                                                                             ccGATAGCGGTCTTTTCGGCTGCCGATTTAATTGCCGCTTTGCCGGCGTCATCAAAACCCGTGGTGCCGATAACGATTTTTTTATGGTTGGCAACACAAAATGCCAAATGTTCCAATGTGCCTT‑CCGGGCGGGTGAAATCAATCAGTAAATCGAAACGATCTTTCACCGCTTTTAAGTCATCGCCGACCTTAATGCCTAAGTTGCCGATCCCCGCCAATTCGCCGGCATCGGCGCCCACCAATGAGGAAcc                                                      <  1:519382/250‑1 (MQ=255)                                                                                                                                                                             ccGATAGCGGTCTTTTCGGCTGCCGATTTAATTGCCGCTTTGCCGGCGTCATCAAAACCCGTGGTGCCGATAACGATTTTTTTATGGTTGGCAACACAAAATGCCAAATGTTCCAATGTGCCTT‑CCGGGCGGGTGAAATCAATCAGTAAATCGAAACGATCTTTCACCGCGTTTAAGTCATCGCCGACCTTAATGCCTAAGTTGCCGATCCCCGCCAATTCGCCGGCATCGGCGCCCACCAATGAGGAAcc                                                      <  1:837788/250‑1 (MQ=255)                                                                                                                                                                                 tAGCGGTCTTTTCGGCTGCCGATTTAATTGCCGCTTTGCCGGCGTCATCAAAACCCGTGGTGCCGATAACGATTTTTTTATGGTTGGCAACACAAAATGCCAAATGTTCCAATGTGCCTT‑CCGGGCGGGTGAAATCAATCAGTAAATCGAAACGATCTTTCTCCGCGTTTAAGTCATCGCCGACCTTAATGCCTAAGTTGCCGATCCCCGCCAATTCGCCGGCATCGGCGCCCACCAATGAGGAACCTTTg                                                  <  1:261109/251‑1 (MQ=255)                                                                                                                                                                                    aatgtgtataagaggcagATTTAATTGCCGCTTTGCGGGCGTCATCAAAACCCGTGGTGCGGATAACGATTTTTTTATGGTTGGCAACACAAAAGGCCAAATGTTCCAATGTGCCTT‑CCGGGCGGGTGAAATCAATCAGTAAATCGAAACGATCTTTCTCCGCGTTTAAGTCATCGCCGACCTTAATGCCTAAGTTGCCGATCCCCGCCAATTCGCCGGCATCGGCGCCCACCAATGAGGAACCTTTgcgc                                               <  1:860297/234‑1 (MQ=255)                                                                                                                                                                                        cTTTTCGGCTGCCGATTTAATTGCCGCTTTGCCGGCGTCATCAAAACCCGTGGTGCCGATAACGATTTTTTTATGGTTGGCAACACAAAATGCCAAATGTTCCAATGTGCCTT‑CCGGGCGGGTGAAATCAATCAGTAAATCGAAACGATCTTTCACCGCGTTTAAGTCATCGCCGACCTTAATGCCTAAGTTGCCGATCCCCGCCAATTCGCCGGCATCGGCGCCCACCAATGAGGAACCTTTGCGCTCaa                                           >  1:596478/1‑251 (MQ=255)                                                                                                                                                                                        cTTTTCGGCTGCCGATTTAATTGCCGCTTTGCCGGCGTCATCAAAACCCGTGGTGCCGATAACGATTTTTTTATGGTTGGCAACACAAAATGCCAAATGTTCCAATGTGCCTT‑CCGGGCGGGTGAAATCAATCAGTAAATCGAAACGATCCTTCACCGCGTTTAAGTCATCGCCGACCTTAATGc                                                                                                             >  1:631766/1‑185 (MQ=255)                                                                                                                                                                                                     gATTTAATTGCCGCTTTGCCGGCGTCATCAAAACCCGTGGTGCCGATAACGATTTTTTTATGGTTGGCAACACAAAATGCCAAATGTTCCAATGTGCCTT‑CCGGGCGGGTGAAATCAATCAGTAAATCGAAACGATCTTTCACCGCGTTTAAGTCATCGCCGACCTTAATGCCTAAGTTGCCGATCCCCGCCAATTCGCCGGCATCGGCGCCCACCAATGAGGAACCTTTGCGCTCAAACGCCGCACCTaa                              >  1:16679/1‑251 (MQ=255)                                                                                                                                                                                                      aTTTAATTGCCGCTTTGCCGGCGTCATCAAAACCCGTGGTGCCGATAACGATTTTTTTATGGTTGGCAACACAAAATGCCAAATGTTCCAATGTGCCTT‑CCGGGCGGGTGAAATCAATCAGTAAATCGAAACGATCTTTCACCGCGTTTAAGTCATCGCCGACCTTAATGCCTAAGTTGCCGATCCCCGCCAATTCGCCGGCATCGGCGCCCACCAATGAGGAACCTTTGCGCTCAAACGCCGCACCTAAt                             >  1:716172/1‑251 (MQ=255)                                                                                                                                                                                                        ttAATTGCCGCTTTGCCGGCGTCATCAAAACCCGTGGTGCCGATAACGATTTTTTTATGGTTGGCAACACAAAATGCCAAATGTTCCAATGTGCCTT‑CCGGGCGGGTGAAATCAATCAGTAAATCGAAACGATCTTTCACCGCGTTTAAGTCATCGCCGACCTTAATGCCTAAGTTGCCGATCCCCGCCAATTCGCCGGCATCGGCGCCCACCAATGAGGAACCTTTGCGCTCAAACGCCGCACCTAATTc                           <  1:118957/251‑1 (MQ=255)                                                                                                                                                                                                        ttAATTGCCGCTTTGCCGGCGTCATCAAAACCCGTGGTGCCGATAACGATTTTTTTATGGTTGGCAACACAAAATGCCAAATGTTCCAATGTGCCTT‑CCGGGCGGGTGAAATCAATCAGTAAATCGAAACGATCTTTCACCGCGTTTAAGTCATCGCCGACCTTAATGCCTAAGTTGCCGATCCCCGCCAATTCGCCGGCATCGGCGCCCACCAATGAGGAACCTTTGCGCTCAAACGCCGCACCTAATTc                           <  1:49808/251‑1 (MQ=255)                                                                                                                                                                                                          aaTTGCCGCTTTGCCGGCGTCATCAAAACCCGTGGTGCCGATAACGATTTTTTTATGGTTGGCAACACAAAATGCCAAATGTTCCAATGTGCCTT‑CCGGGCGGGTGAAATCAATCAGTAAATCGAAACGATCTTTCACCGCGTTTAAGTCATCGCCGACCTTAATGCCTAAGTTGCCGATCCCCGCCAATTCGCCGGCATCGGCGCCCACCAATGAGGAACCTTTGCGCTCAAACGCCGCACCTAATTCCa                         <  1:872901/251‑1 (MQ=255)                                                                                                                                                                                                               ccGCTTTGCCGGCGTCATCAAAACCCGTGGTGCCGATAACGATTTTTTTATGGTTGGCAACACAAAATGCCAAATGTTCCAATGTGCCTT‑CCGGGCGGGTGAAATCAATCAGTAAATCGAAACGATCTTTCACCGCGTTTAAGTCATCGCCGACCTTAATGCCTAAGTTGCCGATCCCCGCCAATTCGCCGGCATCGGCGCCCACCAATGAGGAACCTTTGCGCTCAAACGCCGCACCTAATTCCACGcc                     <  1:896599/250‑1 (MQ=255)                                                                                                                                                                                                                    ttGCCGGCGTCATCAAAACCCGTGGTGCCGATAACGATTTTTTTATGGtt                                                                                                                                                                                                                         >  1:884101/1‑50 (MQ=255)                                                                                                                                                                                                                    ttGCCGGCGTCATCAAAACCCGTGGTGCCGATAACGATTTTTTTATGGTTGGCAACACAAAATGCCAAATGTTCCAATGTGCCTT‑CCGGGCGGGTGAAATCAATCAGTAAATCGAAACGATCTTTCACCGCGTTTAAGTCATCGCCGACCTTAATGCCTAAGTTGCCGATCCCCGCCAATTCGCCGGCATCGGCGCCCACCAATGAgg                                                          >  1:597447/1‑208 (MQ=255)                                                                                                                                                                                                                     tGCCGGCGTCATCAAAACCCGTGGTGCCGATAACGATTTTTTTATGGTTGGCAACACAAAATGCCAAATGTTCCAATGTGCCTT‑CCGGGCGGGTGAAATCAATCAGTAAATCGAAACGATCTTTCACCGCGTTTAAGTCATCGCCGACCTTAATGCCTAAGTTGCCGATCCCCGCCAATTCGCCGGCATCGGCGCCCACCAATGAGGAACCTTTGCGCTCAAACGCCGCACCTAATTCCACGCCTTCcgcg              <  1:92564/251‑1 (MQ=255)                                                                                                                                                                                                                       ccGGCGTCATCAAAACCCGTGGTGCCGATAACGATTTTTTTATGGTTGGCAACACAAAATGCCAAATGTTCCAATGTGCCTT‑CCGGGCGGGTGAAATCAATCAGTAAATCGAAACGATCTTTCACCGCGTTTAAGTCATCGCCGACCTTAATGCCTAAGTTGCCGATCCCCGCCAATTCGCCGGCATCGGCGCCCACCAATGAGGAACCTTTGCGCTCAAACGCCGCACCTAATTCCACGCCTTCCGCGtt            <  1:591739/251‑1 (MQ=255)                                                                                                                                                                                                                             tcatcaAAACCCGTGGTGCCGATAACGATTTTTTTATGGTTGGCAACACAAAATGCCAAATGTTCCAATGTGCCTT‑CCGGGTGGGTGAAATCAATCAGTAAATCGAAACGATCTTTCACCGCGTTTAAGTCATCGCCGACCTTAATGCCTAAGTTGCCGATCCCCGCCAATTCGCCGGCATCGGCGCCCACCAATGAGGAACCTTTGCGCTCAAACGCCGCACCTAATTCCACGCCTTCCGCGTTATGTAc      <  1:432415/251‑1 (MQ=255)                                                                                                                                                                                                                             tcatcaAAACCCGTGGTGCCGATAACGATTTTTTTATGGTTGGCAACACAAAATGCCAAATGTTCCAATGTGCCTT‑CCGGGCGGGTGAAATCAATCAGTAAATCGAAACGATCTTTCACCGCGTTTAAGTCATCGCCGACCTTAATGCCTAAGTTGCCGATCCCCGCCAATTCGCCGGCATCGGCGCCCACCAATGAGGAACCTTTGCGCTCAAACGCCGCACCTAATTCCACGCCTTCCGCGTTATGTAc      <  1:177514/251‑1 (MQ=255)                                                                                                                                                                                                                             tcatcaAAACCCGTGGTGCCGATAACGATTTTTTTATGGTTGGCAACACAAAATGCCAAATGTTCCAATGTGCCTT‑CCGGGCGGGTGAAATCAATCAGTAAATCGAAACGATCTTTCACCGCGTTTAAGTCATCGCCGACCTTAATGCCTAAGTTGCCGATCCCCGCCAATTCGCCGGCATCGGCGCCCACCAATGAGGAACCTTTGCGCTCAAACGCCGCACCTAATTCCACGCCTTCCGCGTTATGTAc      <  1:178867/251‑1 (MQ=255)                                                                                                                                                                                                                                tcaAAACCCGTGGTGCCGATAACGATTTTTTTATGGTTGGCAACACAAAATGCCAAATGTTCCAATGTGCCTT‑CCGGGCGGGTGAAATCAATCAGTAAATCGAAACGATCTTTCAc                                                                                                                                          <  1:477486/116‑1 (MQ=255)                                                                                                                                                                                                                                tcaAAACCCGTGGTGCCGATAACGATTTTTTTATGGTTGGCAACACAAAATGCCAAATGTTCCAATGTGCCTT‑CCGGGCGGGTGAAATCAATCAGTAAATCGAAACGATCTTTCACCGCGTTTAAGTCATCGCCGCCCTTAATGCCTAAGTTGCCGATCCCCGCCAATTCGCCGGCATCGGCGCCCACCAATGAGGAACCTTTGCGCTCAAACGCCGCACCTAATTCCACGCCTTCCGCGTTATGTACCGc   <  1:496309/251‑1 (MQ=255)                                                                                                                                                                                                                                 caAAACCCGTGGTGCCGATAACGATTTTTTTATGGTTGGCAACACAAAATGCCAAATGTTCCAATGTGCCTT‑CCGGGCGGGTGAAATCAATCAGTAAATCGAAACGATCTTTCACCGCGTTTAAGTCATCGCCGACCTTAATGCCTAAGTTGCCGATCCCCGCCAATTCGCCGGCATCGGCGCCCACCAATGAGGAACCTTTGCGCTCAAACGCCGCACCTAATTCCACGCCTTCCGCGTTATGTACCGcc  <  1:691796/251‑1 (MQ=255)                                                                                                                                                                                                                                      cccGTGGTGCCGATAACGATTTTTTTATGGTTGGCAACACAAAATGCCAAATGTTCCAATGTGCCTT‑CCGGGCGGGTGAAATCAATCAGTAAATCGAAACGATCTTTCACCGCGTTTAAGTCATCGCCGACCTTAATGCCTAAGTTGCCGATCCCCGCCAATTCGCCGGCATCGGCGCCCACCAATGAGGAACCTTTGCGCTCAAAcgc                                       >  1:378035/1‑209 (MQ=255)                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                       GGTTTTGGCAATATGTTCGCCCATGGAAAGCGCGGTACCGGACGGCGCATCGACTTTATAGCGGTGGTGCGCTTCGATGATTTCGATGTCGCAATAATCGCCCATCACCTTGGCGGCTTTTTCCAATAATTTAAAGACCAAATTCACGCCGACACTGAAATTTGAAGCAAACACGATAGCGGTCTTTTCGGCTGCTGATTTAATTGCCGCTTTGCCGGCGTCATCAAAACCCGTGGTGCCGATAATGATTTTTTTATGGTTGGCAACACAAAATGCCAAATGTTCCAATGTGCCTT‑CCGGGCGGGTGAAATCAATCAGTAAATCGAAACGATCTTTCACCGCGTTTAAGTCATCGCCGACCTTAATGCCTAAGTTGCCGATTCCCGCCAATTCGCCGGCATCGGCGCCCACCAATGAGGAACCTTTGCGCTCAAACGCCGCACCTAATTCCACGCCTTCCGCGTTATGTACCGCC  >  1/1616337‑1616811

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑