Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,616,532	T→C	intergenic (‑171904/‑25232)	unknown ← / → unknown	tRNA‑Met‑CAT/tRNA‑Gly‑GCC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,616,532	0	T	C	100.0%	213.1 / NA	77	intergenic (‑171904/‑25232)	unknown/unknown	tRNA‑Met‑CAT/tRNA‑Gly‑GCC
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (44/33);  total (44/33)

TAATACCATCGCGACAAAATACGCCGTGGGTTTTCAGATCCCGTCCGAGGGTTTTGGCAATATGTTCGCCCATGGAAAGCGCGGTACCGGACGGCGCATCGACTTTATAGCGGTGGTGCGCTTCGATGATTTCGATGTCGCAATAATCGCCCATCACCTTGGCGGCTTTTTCCAATAATTTAAAGACCAAATTCACGCCGACACTGAAATTTGAAGCAAACACGATAGCGGTCTTTTCGGCTGCTGATTTAATTGCCGCTTTGCCGGCGTCATCAAAACCCGTGGTGCCGATAATGATTTTTTTATGGTTGGCAACACAAAATGCCAAATGTTCCAATGTGCCTT‑CCGGGCGGGTGAAATCAATCAGTAAATCGAAACGATCTTTCACCGCGTTTAAGTCATCGCCGACCTTAATGCCTAAGTTGCCGATTCCCGCCAATTCGCCGGCATCGGCGCCCACCAATGAGGAACCTTTGCGCTCAA  >  1/1616288‑1616770                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                taataCCATCGCGACAAAATACGCCGTGGGTTTTCAGATCCCGTCCGAGGGTTTTGGCAATATGTTCGCCCATGGAAAGCGCGGTACCGGACGGCGCATCGACTTTATAGCGGTGGTGCGCTTCGATGATTTCGATGTCGCAATAATCGCCCATCACCTTGGCGGCTTTTTCCAATAATCTAAAGACCAAATTCACGCCGACACTGAAATTTGAAGCAAACACGATAGCGGTCTTTTCGGCTGCCGATTTa                                                                                                                                                                                                                                           >  1:211376/1‑251 (MQ=255)            cgACAAAATACGCCGTGGGTTTTCAGATCCCGTCCGAGGGTTTTGGCAATATGTTCGCCCATGGAAAGCGCGGTACCGGACGGCGCATCGACTTTATAGCGGTGGTGCGCTTCGATGATTTCGATGTCGCAATAATCGCCCATCACCTTGGCGGCTTTTTCCAATAATTTAAAGACCAAATTCACGCCGACACTGAAATTTGAAGCAAACACGATAGCGGTCTTTTCGGCTGCCGATTTAATTGCCGCttt                                                                                                                                                                                                                                >  1:45777/1‑251 (MQ=255)                       gCCGTGGGTTTTCAGATCCCGTCCGAGGGTTTTGGCAATATGTTCGCCCATGGAAAGCGCGGTACCGGACGGCGCATCGACTTTATAGCGGTGGTGCGCTTCGATGATTTCGATGTCGCAATAATCGCCCATCACCTTGGCGGCTTTTTCCAATAATTTAAAGACCAAATTCACGCCGACACTGAAATTTGAAGCAAACACGATAGCGGTCTTTTCGGCTGCCGATTTAATTGCCGCTTTGCCGGCGtcat                                                                                                                                                                                                                     >  1:571202/1‑251 (MQ=255)                            gggTTTTCAGATCCCGTCCGAGGGTTTTGGCAATATGTTCGCCCATGGAAAGCGCGGTACCGGACGGCGCATCGACTTTATAGCGGTGGTGCGCTTCGATGATTTCGATGTCGCAATAATCGCCCATCACCTTGGCGGCTTTTTCCAATAATTTAAAGACCAAATTCACGCCGACACTGAAATTTGAAGCAAACACGATAGCGGTCTTTTCGGCTGCCGATTTAATTGCCGc                                                                                                                                                                                                                                   >  1:802969/1‑232 (MQ=255)                            gggTTTTCAGATCCCGTCCGAGGGTTTTGGCAATATGTTCGCCCATGGAAAGCGCGGTACCGGACGGCGCATCGACTTTATAGCGGTGGTGCGCTTCGATGATTTCGATGTCGCAATAATCGCCCATCACCTTGGCGGCTTTTTCCAATAATTTAAAGACCAAATTCACGCCGACACTGAAATTTGAAGCAAACACGATAGCGGTCTTTTCGGCTGCCGATTTAATTGCCGCTTTGCCGGCGTCATCaaaa                                                                                                                                                                                                                >  1:460923/1‑251 (MQ=255)                            gggTTTTCAGATCCCGTCCGAGGGTTTTGGCAATATGTTCGCCCATGGAAAGCGCGGTACCGGACGGCGCATCGACTTTATAGCGGTGGTGCGCTTCGATGATTTCGATGTCGCAATAATCGCCCATCACCTTGGCGGCTTTTTCCAATAATTTAAAGACCAAATTCACGCCGACACTGAAATTTGAAGCAAACACGATAGCGGTCTTTTCGGCTGCCGATTTAATTGCCGCTTTGCCGGCGTCATCaaaa                                                                                                                                                                                                                >  1:479383/1‑251 (MQ=255)                             ggTTTTCAGATCCCGTCCGAGGGTTTTGGCAATATGTTCGCCCATGGAAAGCGCGGTACCGGACGGCGCATCGACTTTATAGCGGTGGTGCGCTTCGATGATTTCGATGTCGCAATAATCGCCCATCACCTTGGCGGCTTTTTCCAATAATTTAAAGACCAAATTCACGCCGACACTGAAATTTGAAGCAAACACGATAGCGGTCTTTTCGGCTGCCGATTTAATTGCCGCTTTGCCGGCGTCATCaaaa                                                                                                                                                                                                                >  1:320091/1‑250 (MQ=255)                             ggTTTTCAGATCCCGTCCGAGGGTTTTGGCAATATGTTCGCCCATGGAAAGCGCGGTACCGGACGGCGCATCGACTTTATAGCGGTGGTGCGCTTCGATGATTTCGATGTCGCAATAATCGCCCATCACCTTGGCGGCTTTTTCCAATAATTTAAAGACCAAATTCACGCCGACACTGAAATTTGAAGCAAACACGATAGCGGTCTTTTCGGCTGCCGATTTAATTGCCGCTTTGCCGGCGTCATCaaaa                                                                                                                                                                                                                >  1:353079/1‑250 (MQ=255)                             ggTTTTCAGATCCCGTCCGAGGGTTTTGGCAATATGTTCGCCCATGGAAAGCGCGGTACCGGACGGCGCATCGACTTTATAGCGGTGGTGCGCTTCGATGATTTCGATGTCGCAATAATCGCCCATCACCTTGGCGGCTTTTTCCAATAATTTAAAGACCAAATTCACGCCGACACTGAAATTTGAAGCAAACACGATAGCGGTCTTTTCGGCTGCCGATTTAATTGCCGCTTTGCCGGCGTCATCaaaa                                                                                                                                                                                                                >  1:242244/1‑250 (MQ=255)                                 ttCAGATCCCGTCCGAGGGTTTTGGCAATATGTTCGCCCATGGAAAGCGCGGTACCGGACGGCGCATCGACTTTATAGCGGTGGTGCGCTTCGATGATTTCGATGTCGCAATAATCGCCCATCACCTTGGCGGCTTTTTCCAATAATTTAAAGACCAAATTCACGCCGACACTGAAATTTGAAGCAAACACGATAGCGGTCTTTTCGGCTGCCGATTTAATTGCCGCTTTGCCGGCGTCATCAAAACCCgt                                                                                                                                                                                                           >  1:249970/1‑251 (MQ=255)                                     gATCCCGTCCGAGGGTTTTGGCAATATGTTCGCCCATGGAAAGCGCGGTACCGGACGGCGCATCGACTTTATAGCGGTGGTGCGCTTCGATGATTTCGATGTCGCAATAATCGCCCATCACCTTGGCGGCTTTTTCCAATAATTTAAAGACCAAATTCACGCCGACACTGAAATTTGAAGCAAACACGATAGCGGTCTTTTCGGCTGCCGATTTAATTGCCGCTTTGCCGGCGTCATCAAAAc                                                                                                                                                                                                               <  1:684463/243‑1 (MQ=255)                                     gATCCCGTCCGAGGGTTTTGGCAATATGTTCGCCCATGGAAAGCGCGGTACCGGACGGCGCATCGACTTTATAGCGGTGGTGCGCTTCGATGATTTCGATGTCGCAATAATCGCCCATCACCTTGGCGGCTTTTTCCAATAATTTAAAGACCAAATTCACGCCGACACTGAAATTTGAAGCAAACACGATAGCGGTCTTTTCGGCTGCCGATTTAATTGCCGCTTTGCCGGCGTCATCAAAACCCgtggtg                                                                                                                                                                                                       >  1:831480/1‑251 (MQ=255)                                          cGTCCGAGGGTTTTGGCAATATGTTCGCCCATGGAAAGCGCGGTACCGGACGGCGCATCGACTTTATAGCGGTGGTGCGCTTCGATGATTTCGATGTCGCAATAATCGCCCATCACCTTGGCGGCTTTTTCCAATAATTTAAAGACCAAATTCACGCCGACACTGAAATTTGAAGCAAACACGATAGCGGTCTTTTCGGCTGCCGATTTAATTGCCGCTTTGCCGGCGTCATCAAAACCCGTGGTGCCGAt                                                                                                                                                                                                  >  1:319465/1‑251 (MQ=255)                                           gTCCGAGGGTTTTGGCAATATGTTCGCCCATGGAAAGCGCGGTACCGGACGGCGCATCGACTTTATAGCGGTGGTGCGCTTCGATGATTTCGATGTCGCAATAATCGCCCATCACCTTGGCGGCTTTTTCCAATAATTTAAAGACCAAATTCACGCCGACACTGAAATTTGAAGCAAACACGATAGCGGTCTTTTCGGCTGCCGATTTAATTGCCGCTTTGCCGGCGTCATCAAAACCCGTGGTGCCGATa                                                                                                                                                                                                 >  1:681493/1‑251 (MQ=255)                                                  ggTTTTGGCAATATGTTCGCCCATGGAAAGCGCGGTACCGGACGGCGCATCGACTTTATAGCGGTGGTGCGCTTCGATGATTTCGATGTCGCAATAATCGCCCATCACCTTGGCGGCTTTTTCCAATAATTTAAAGACCAAATTCACGCCGACACTGAAATTTGAAGCAAACACGATAGCGGTCTTTTCGGCTGCCGATTTAATTGCCGCTTTGCCGGCGTCATCAAAACCCGTGGTGCCGATAACGAttt                                                                                                                                                                                          >  1:86861/1‑251 (MQ=255)                                                       tGGCAATATGTTCGCCCATGGAAAGCGCGGTACCGGACGGCGCATCGACTTTAAAGCGGTGGTGCGCTTCGATGATTTCGATGTCGCAATAATCGCCCATCACCTTGGCGGCTTTTTCCAATAATTTAAAGACCAAATTCACGCCGACACTGAAATTTGAAGCAAACACGATAGCGGTCTTTTCGGCTGCCGATTTAATTGCCGCTTTGCCGGCGTCATCAAAACCCGTGGTGCCGATAACGATTTTTTTa                                                                                                                                                                                     <  1:317497/251‑1 (MQ=255)                                                          cAATATGTTCGCCCATGGAAAGCGCGGTACCGGACGGCGCATCGACTTTATAGCGGTGGTGCGCTTCGATGATTTCGATGTCGCAATAATCGCCCATCACCTTGGCGGCTTTTTCCAATAATTTAAAGACCAAATTCACGCCGACACTGAAATTTGAAGCAAACACGATAGCGGTCTTTTCGGCTGCCGATTTAATTGCCGCTTTGCCGGCGTCATCAAAACCCGTGGTGCCGATAACGATTTTTTTATgg                                                                                                                                                                                  <  1:482563/251‑1 (MQ=255)                                                                     cccATGGAAAGCGCGGTACCGGACGGCGCATCGACTTTATAGCGGTGGTGCGCTTCGATGATTTCGATGTCGCAATAATCGCCCATCACCTTGGCGGCTTTTTCCAATAATTTAAAGACCAAATTCACGCCGACACTGAAATTTGAAGCAAACACGATAGCGGTCTTTTCGGCTGCCGATTTAATTGCCGCTTTGCCGGCGTCATCAAAAcc                                                                                                                                                                                                              <  1:253910/212‑1 (MQ=255)                                                                     cccATGGAAAGCGCGGTACCGGACGGCGCATCGACTTTATAGCGGTGGTGCGCTTCGATGATTTCGATGTCGCAATAATCGCCCATCACCTTGGCGGCTTTTTCCAATAATTTAAAGACCAAATTCACGCCGACACTGAAATTTGAAGCAAACACGATAGCGGTCTTTTCGGCTGCCGATTTAATTGCCGCTTTGCCGGCGTCATCAAAACCCGTGGTGCCGATAACGATTTTTTTATGGTTGGCAacac                                                                                                                                                                        <  1:202219/250‑1 (MQ=255)                                                                     cccATGGAAAGCGCGGTACCGGACGGCGCATCGACTTTATAGCGGTGGTGCGCTTCGATGATTTCGATGTCGCAATAATCGCCCATCACCTTGGCGGCTTTTTCCAATAATTTAAAGACCAAATTCACGCCGACACTGAAATTTGAAGCAAACACGATAGCGGTCTTTTCGGCTGCCGATTTAATTGCCGCTTTGCCGGCGTCATCAAAACCCGTGGTGCCGATAACGATTTTTTTATGGTTGGCAAcaca                                                                                                                                                                       >  1:499402/1‑251 (MQ=255)                                                                     cccATGGAAAGCGCGGTACCGGACGGCGCATCGACTTTATAGCGGTGGTGCGCTTCGATGATTTCGATGTCGCAATAATCGCCCATCACCTTGGCGGCTTTTTCCAATAATTTAAAGACCAAATTCACGCCGACACTGAAATTTGAAGCAAACACGATAGCGGTCTTTTCGGCTGCCGATTTAATTGCCGCTTTGCCGGCGTCATCAAAACCCGTGGTGCCGATAACGATTTTTTTATGGTTGGCAAcaca                                                                                                                                                                       >  1:316873/1‑251 (MQ=255)                                                                     cccATGGAAAGCGCGGTACCGGACGGCGCATCGACTTTATAGCGGTGGTGCGCTTCGATGATTTCGATGTCGCAATAATCGCCCATCACCTTGGCGGCTTTTTCCAATAATTTAAAGACCAAATTCACGCCGACACTGAAATTTGAAGCAAACACGATAGCGGTCTTTTCGGCTGCCGATTTAATTGCCGCTTTGCCGGCGTCATCAAAACCCGTGGTGCCGATAACGATTTTTTTATGGTTGGCAAcaca                                                                                                                                                                       >  1:470221/1‑251 (MQ=255)                                                                               gcgcGGTACCGGACGGCGCATCGACTTTATAGCGGTGGTGCGCTTCGATGATTTCGATGTCGCAATAATCGCCCATCACCTTGGCGGCTTTTTCCAATAATTTAAAGACCAAATTCACGCCGACACTGAAATTTGAAGCAAACACGATAGCGGTCTTTTCGGCTGCCGATTTAATTGCCGCTTTGCCGGCGTCATCAAAACCCGTGGTGCCGATAACGATTTTTTTATGGTTGGCAACACAAAATGCCaaa                                                                                                                                                             >  1:117717/1‑251 (MQ=255)                                                                                    gTACCGGACGGCGCATCGACTTTATAGCGGTGGTGCGCTTCGATGATTTCGATGTCGCAATAATCGCCCATCACCTTGGCGGCTTTTTCCAATAATTTAAAGACCAAATTCACGCCGACACTGAAATTTGAAGCAAACACGATAGCGGTCTTTTCGGCTGCCGATTTAATTGCCGCTTTGCCGGCGTCATCAAAACCCGTGGTGCCGATaac                                                                                                                                                                                               <  1:604011/212‑2 (MQ=255)                                                                                    gTACCGGACGGCGCATCGACTTTATAGCGGTGGTGCGCTTCGATGATTTCGATGTCGCAATAATCGCCCATCACCTTGGCGGCTTTTTCCAATAATTTAAAGACCAAATTCACGCCGACACTGAAATTTGAAGCAAACACGATAGCGGTCTTTTCGGCTGCCGATTTAATTGCCGCTTTGCCGGCGTCATCAAAACCCGTGGTGCCGATAACGATTTTTTTATgg                                                                                                                                                                                  >  1:886353/1‑225 (MQ=255)                                                                                    gTACCGGACGGCGCATCGACTTTATAGCGGTGGTGCGCTTCGATGATTTCGATGTCGCAATAATCGCCCATCACCTTGGCGGCTTTTTCCAATAATTTAAAGACCAAATTCACGCCGACACTGAAATTTGAAGCAAACACGATAGCGGTCTTTTCGGCTGCCGATTTAATTGCCGCTTTGCCGGCGTCATCAAAACCCGTGGTGCCGATAACGATTTTTTTATGGTTGGCAACACAAAATGCCAAATGtt                                                                                                                                                         >  1:449103/1‑250 (MQ=255)                                                                                    gTACCGGACGGCGCATCGACTTTATAGCGGTGGTGCGCTTCGATGATTTCGATGTCGCAATAATCGCCCATCACCTTGGCGGCTTTTTCCAATAATTTAAAGACCAAATTCACGCCGACACTGAAATTTGAAGCAAACACGATAGCGGTCTTTTCGGCTGCCGATTTAATTGCCGCTTTGCCGGCGTCATCAAAACCCGTGGTGCCGATAACGATTTTTTTATGGTTGGCAACACAAAATGCCAAATGtt                                                                                                                                                         >  1:640287/1‑250 (MQ=255)                                                                                          gACGGCGCATCGACTTTATAGCGGTGGTGCGCTTCGATGATTTCGATGTCGCAATAATCGCCCATCACCTTGGCGGCTTTTTCCAATAATTTAAAGACCAAATTCACGCCGACACTGAAATTTGAAGCAAACACGATAGCGGTCTTTTCGGCTGCCGATTTAATTGCCGCTTTGCCGGCGTCATCAAAACCCGTGGTGCCGATAACGATTTTTTTATGGTTGGCAACACAAAATGCCAAATGTTCCAAtg                                                                                                                                                   <  1:209740/250‑1 (MQ=255)                                                                                              gcgcATCGACTTTATAGCGGTGGTGCGCTTCGATGATTTCGATGTCGCAATAATCGCCCATCACCTTGGCGGCTTTTTCCAATAATTTAAAGACCAAATTCACGCCGACACTGAAATTTGAAGCAAACACGATAGCGGTCTTTTCGGCTGCCGATTTAATTGCCGCTTTGCCGGCGTCATCAAAACCCGTGGTGCCGATAACGATTTTTTTATGGTTGGCAACACAAAATg                                                                                                                                                                  <  1:132080/231‑1 (MQ=255)                                                                                                      aCTTTATAGCGGTGGTGCGCTTCGATGATTTCGATGTCGCAATAATCGCCCATCACCTTGGCGGCTTTTTCCAATAATTTAAAGACCAAATTCACGCCGACACTGAAATTTGAAGCAAACACGATAGCGGTCTTTTCGGCTGCCGATTTAATTGCCGCTTTGCCGGCGTCATCAAAACCCGTGGTGCCGATAACGAt                                                                                                                                                                                            <  1:23605/197‑1 (MQ=255)                                                                                                      aCTTTATAGCGGTGGTGCGCTTCGATGATTTCGATGTCGCAATAATCGCCCATCACCTTGGCGGCTTTTTCCAATAATTTAAAGACCAAATTCACGCCGACACTGAAATTTGAAGCAAACACGATAGCGGTCTTTTCGGCTGCCGATTTAATTGCCGCTTTGCCGGCGTCATCAAAACCCGTGGTGCCGATAACGATTTTTTTATgg                                                                                                                                                                                  >  1:250437/1‑207 (MQ=255)                                                                                                      aCTTTATAGCGGTGGTGCGCTTCGATGATTTCGATGTCGCAATAATCGCCCATCACCTTGGCGGCTTTTTCCAATAATTTAAAGACCAAATTCACGCCGACACTGAAATTTGAAGCAAACACGATAGCGGTCTTTTCGGCTGCCGATTTAATTGCCGCTTTGCCGGCGTCATCAAAACCCGTGGTGCCGATAACGATTTTTTTATGGTTGGCAACACAAAATGCCAAATGTTCCAATGTGCCTT‑Ccgggcg                                                                                                                                     >  1:248903/1‑251 (MQ=255)                                                                                                       cTTTATAGCGGTGGTGCGCTTCGATGATTTCGATGTCGCAATAATCGCCCATCACCTTGGCGGCTTTTTCCAATAATTTAAAGACCAAATTCACGCCGACACTGAAATTTGAAGCAAACACGATAGCGGTCTTTTCGGCTGCCGATTTAATTGCCGCTTTGCCGGCGTCATCAAAACCCGTGGTGCCGATAACGATTTTTTTATGGTTGGCAACACAAAATGCCAAATGTTCCAATGTGCCTT‑Ccgggcgg                                                                                                                                    >  1:212593/1‑251 (MQ=255)                                                                                                         ttATAGCGGTGGTGCGCTTCGATGATTTCGATGTCGCAATAATCGCCCATCACCTTGGCGGCTTTTTCCAATAATTTAAAGACCAAATTCACGCCGACACTGAAATTTGAAGCAAACACGATAGCGGTCTTTTCGGCTGCCGATTTAATTGCCGCTTTGCCGGCGTCATCAAAACCCGTGGTGCCGATAACGATTTTTTTATGGTTGGCAACACAAAATGCCAAATGTTCCAATGTGCCTT‑CCGGGCGGGt                                                                                                                                  >  1:806613/1‑251 (MQ=255)                                                                                                           ataGCGGTGGTGCGCTTCGATGATTTCGATGTCGCAATAATCGCCCATCACCTTGGCGGCTTTTTCCAATAATTTAAAGACCAAATTCACGCCGACACTGAAATTTGAAGCAAACACGATAGCGGTCTTTTCGGCTGCCGATTTAATTGCCGCTTTGCCGGCGTCATCAAAAcc                                                                                                                                                                                                              <  1:197615/174‑1 (MQ=255)                                                                                                              gCGGTGGTGCGCTTCGATGATTTCGATGTCGCAATAATCGCCCATCACCTTGGCGGCTTTTTCCAATAATTTAAAGACCAAATTCACGCCGACACTGAAATTTGAAGCAAACACGATAGCGGTCTTTTCGGCTGCCGATTTAATTGCCGCTTTGCCGGCGTCATCAAAACCCGTGGTGCCGATAACGATTTTTTTATGGTTGGCAACACAAAATGCCAAATGTTCCAATGTGCCTT‑CCGGGCGGGTGAaat                                                                                                                             >  1:564040/1‑251 (MQ=255)                                                                                                               cGGTGGTGCGCTTCGATGATTTCGATGTCGCAATAATCGCCCATCACCTTGGCGGCTTTTTCCAATAATTTAAAGACCAAATTCACGCCGACACTGAAGTTTGAAGCAAACACGATAGCGGTCTTTTCGGCTGCCGATTTAATTGCCGCTTTGCCGGCGTCATCAAAACCCGTGGTGCCGATAACGATTTTTTTATGGTTGGCAACACAAAATGCCAAATGTTCCAATGTGCCTT‑CCGGGCGGGTGAaatc                                                                                                                            <  1:564329/251‑1 (MQ=255)                                                                                                                 gtggtgCGCTTCGATGATTTCGATGTCGCAATAATCGCCCATCACCTTGGCGGCTTTTTCCAATAATTTAAAGACCAAATTCACGCCGACACTGAAATTTGAAGCAAACACGATAGCGGTCTTTTCGGCTGCCGATTTAATTGCCGCTTTGCCGGCGTCATCAAAACCCGTGGTGCCGATAACGATTTTTTTATGGTTGGCAACACAAAATGc                                                                                                                                                                 >  1:283933/1‑213 (MQ=255)                                                                                                                 gtggtgCGCTTCGATGATTTCGATGTCGCAATAATCGCCCATCACCTTGGCGGCTTTTTCCAATAATTTAAAGACCAAATTCACGCCGACACTGAAATTTGAAGCAAACACGATAGCGGTCTTTTCGGCTGCCGATTTAATTACCGCTTTGCCGGCGTCATCAAAACCCGTGGTGCCGATAACGATTTTTTTATGGTTGGCAACACAAAATGCCAAATGTTCCAATGTGCCTT‑CCGGGCGGGTGAaatcaa                                                                                                                          >  1:83183/1‑251 (MQ=255)                                                                                                                                 atTTCGATGTCGCAATAATCGCCCATCACCTTGGCGGCTTTTTCCAATAATTTAAAGACCAAATTCACGCCGACACTGAAATTTGAAGCAAACACGATAGCGGTCTTTTCGGCTGCCGATTTAATTGCCGCTTTGCCGGCGTCATCAAAACCCGTGGTGCCGATAACGATTTTTTTATGGTTGGCAACACAAAATGCCAAATGTTCCAATGTGCCTT‑CCGGGCGGGTGAAATCAATCAGTAAATCGAAAc                                                                                                           <  1:727523/250‑1 (MQ=255)                                                                                                                                        tGTCGCAATAATCGCCCATCACCTTGGCGGCTTTTTCCAATAATTTAAAGACCAAATTCACGCCGACACTGAAATTTGAAGCAAACACGATAGCGGTCTTTTCGGCTGCCGATTTAATTGCCGCTTTGCCGGCGTCATCAAAACCCGTGGTGCCGATAACGATTTTTTTATGGTTGGCAACACAAAATGCCAAATGTTCCAATGTGCCTT‑CCGGGCGGGTGAAATCAATCAGTAAATCGAAACGATCTTTc                                                                                                   <  1:479651/251‑1 (MQ=255)                                                                                                                                         gTCGCAATAATCGCCCATCACCTTGGCGGCTTTTTCCAATAATTTAAAGACCAAATTCACGCCGACACTGAAATTTGAAGCAAACACGATAGCGGTCTTTTCGGCTGCCGATTTAATTGCCGCTTTGCCGGCGTCATCAAAACCCGTGGTGCCGATAACGATTTTTTTATGGTTGGCAACACAAAATGCCAAATGTTCCAATGTGCCTT‑CCGGGCGGGTGAAATCAATCAGTAAATCGAAACGATCTTTCa                                                                                                  >  1:539429/1‑251 (MQ=255)                                                                                                                                          tCGCAATAATCGCCCATCACCTTGGCGGCTTTTTCCAATAATTTAAAGACCAAATTCACGCCGACACTGAAATTTGAAGCAAACACGATAGCGGTCTTTTCGGCTGCCGATTTAATTGCCGCTTTGCCGGCGTCATCAAAACCCGTGGTGCCGATAACGATTTTTTTATGGTTGGCAACACAAAATGCCAAATGTTCCAATGTGCCTT‑CCGGGCGGGTGAAATCAATCAGTAAATCGAAACGATCTTTCAc                                                                                                 <  1:158407/251‑1 (MQ=255)                                                                                                                                          tCGCAATAATCGCCCATCACCTTGGCGGCTTTTTCCAATAATTTAAAGACCAAATTCACGCCGACACTGAAATTTGAAGCAAACACGATAGCGGTCTTTTCGGCTGCCGATTTAATTGCCGCTTTGCCGGCGTCATCAAAACCCGTGGTGCCGATAACGATTTTTTTATGGTTGGCAACACAAAATGCCAAATGTTCCAATGTGCCTT‑CCGGGCGGGTGAAATCAATCAGTAAATCGAAACGATCTTTCAc                                                                                                 <  1:600100/251‑1 (MQ=255)                                                                                                                                          tCGCAATAATCGCCCATCACCTTGGCGGCTTTTTCCAATAATTTAAAGACCAAATTCACGCCGACACTGAAATTTGAAGCAAACACGATAGCGGTCTTTTCGGCTGCCGATTTAATTGCCGCTTTGCCGGCGTCATCAAAACCCGTGGTGCCGATAACGATTTTTTTATGGTTGGCAACACAAAATGCCAAATGTTCCAATGTGCCTT‑CCGGGCGGGTGAAATCAATCAGTAAATCGAAACGATCTTTCAc                                                                                                 <  1:602439/251‑1 (MQ=255)                                                                                                                                          tCGCAATAATCGCCCATCACCTTGGCGGCTTTTTCCAATAATTTAAAGACCAAATTCACGCCGACACTGAAATTTGAAGCAAACACGATAGCGGTCTTTTCGGCTGCCGATTTAATTGCCGCTTTGCCGGCGTCATCAAAACCCGTGGTGCCGATAACGATTTTTTTATGGTTGGCAACACAAAATGCCAAATGTTCCAATGTGCCTT‑CCGGGCGGGTGAAATCAATCAGTAAATCGAAACGATCTTTCAc                                                                                                 <  1:104952/251‑1 (MQ=255)                                                                                                                                                taatCGCCCATCACCTTGGCGGCTTTTTCCAATAATTTAAAGACCAAATTCACGCCGACACTGAAATTTGAAGCAAACACGATAGCGGTCTTTTCGGCTGCCGATTTAATTGCCGCTTTGCCGGCGTCATCAAAACCCGTGGTGCCGATAACGATTTTTTTATGGTTGGCAACACAAAATGCCAAATGTTCCAATGTGCCTT‑CCGGGCGGGTGAAATCAATCAGTAAATCGAAACGATCTTTCAc                                                                                                 <  1:399135/245‑1 (MQ=255)                                                                                                                                                  atCGCCCATCACCTTGGCGGCTTTTTCCAATAATTTAAAGACCAAATTCACGCCGACACTGAAATTTGAAGCAAACACGATAGCGGTCTTTTCGGCTGCCGATTTAATTGCCGCTTTGCCGGCGTCATCAAAACCCGTGGTGCCGATAACGATTTTTTTATGGTTGGCAACACAAAATGCCAAATGTTCCAATGTGCCTT‑CCGGGCGGGTGAAATCAATCAGTAAATCGAAACGATCTTTCACCGCGTTTa                                                                                         >  1:778852/1‑251 (MQ=255)                                                                                                                                                    cGCCCATCACCTTGGCGGCTTTTTCCAATAATTTAAAGACCAAATTCACGCCGACACTGAAATTTGAAGCAAACACGATAGCGGTCTTTTCGGCTGCCGATTTAATTGCCGCTTTGCCGGCGTCATCAAAACCCGTGGTGCCGATAACGATTTTTTTATGGTTGGCAACACAAAATGCCAAATGTTCCAATGTGCCTT‑CCGGGCGGGTGAAATCAATCAGTAAATCGAAACGATCTTTCACCGCGTTTAAg                                                                                       <  1:179860/251‑1 (MQ=255)                                                                                                                                                     gCCCATCACCTTGGCGGCTTTTTCCAATAATTTAAAGACCAAATTCACGCCGACACTGAAATTTGAAGCAAACACGATAGCGGTCTTTTCGGCTGCCGATTTAATTGCCGCTTTGCCGGCGTCATCAAAACCCGTGGTGCCGATAACGATTTTTTTATGGTTGGCAACACAAAATGCCAAATGTTCCAATGTGCCTT‑CCGGGCGGGTGAAATCAATCAGTAAATCGAAACGATCTTTCACCGCGTTTAAGt                                                                                      >  1:768174/1‑251 (MQ=255)                                                                                                                                                      cccATCACCTTGGCGGCTTTTTCCAATAATTTAAAGACCAAATTCACGCCGACACTGAAATTTGAAGCAAACACGATAGCGGTCTTTTCGGCTGCCGATTTAATTGCCGCTTTGCCGGCGTCATCAAAACCCGTGGTGCCGATAACGATTTTTTTATGGTTGGCAACACAAAATGCCAAATGTTCCAATGTGCCTT‑CCGGGCGGGTGAAATCAATCAGTAAATCGAAACGATCTTTCACCGCGTTTAAGt                                                                                      <  1:285323/250‑1 (MQ=255)                                                                                                                                                          tCACCTTGGCGGCTTTTTCCAATAATTTAAAGACCAAATTCACGCCGACACTGAAATTTGAAGCAAACACGATAGCGGTCTTTTCGGCTGCCGATTTAATTGCCGCTTTGCCGGCGTCATCAAAACCCGTGGTGCCGATAACGATTTTTTTATGGTTGGCAACACAAAATGCCAAATGTTCCAATGTGCCTT‑CCGGGCGGGTGAaatcaatc                                                                                                                        >  1:11784/1‑212 (MQ=255)                                                                                                                                                             ccTTGGCGGCTTTTTCCAATAATTTAAAGACCAAATTCACGCCGACACTGAAATTTGAAGCAAACACGATAGCGGTCTTTTCGGCTGCCGATTTAATTGCCGCTTTGCCGGCGTCATCAAAACCCGTGGTGCCGATAACGATTTTTTTATGGTTGGCAACACAAAATGCCAAATGTTCCAATGTGCCTT‑CCGGGCGGGTGAAATCAATCAGTAAATCGAAACGATCTTTCACCGCGTTTAAGTCATCGCCg                                                                              >  1:36933/1‑251 (MQ=255)                                                                                                                                                             ccTTGGCGGCTTTTTCCAATAATTTAAAGACCAAATTCACGCCGACACTGAAATTTGAAGCAAACACGATAGCGGTCTTTTCGGCTGCCGATTTAATTGCCGCTTTGCCGGCGTCATCAAAACCCGTGGTGCCGATAACGATTTTTTTATGGTTGGCAACACAAAATGCCAAATGTTCCAATGTGCCTT‑CCGGGCGGGTGAAATCAATCAGTAAATCGAAACGATCTTTCACCGCGTTTAAGTCATCGCCg                                                                              >  1:366235/1‑251 (MQ=255)                                                                                                                                                             ccTTGCCGGCTTTTTCCACTAATTTACAGACCAAATTCACGCCGACACTGCAATTTGAAGCAAACACGACAGCGGTCTTTTCGGCTGCCGATTCACTTGCCGCTTTGCCGGCGTCATCAAAACCCGTGGTGCCGATAACGATTTTTTTATGGTTGGCAACACAAAATGACAAATGTTCCAATGTGCCTT‑CCGGGCGGGTGAaatc                                                                                                                            >  1:872972/1‑205 (MQ=255)                                                                                                                                                                tGGCGGCTTTTTCCAATAATTTAAAGACCAAATTCACGCCGACACTGAAATTTGAAGCAAACACGATAGCGGTCTTTTCGGCTGCCGATTTAATTGCCGCTTTGCCGGCGTCATCAAAACCCGTGGTGCCGATAACGATTTTTTTATGGTTGGCAACACAAAATGCCAAATGTTCCAATGTGCCTT‑CCGGGCGGGTGAAATCAATCAGTAAATCGAAACGATCTTTCAc                                                                                                 >  1:147158/1‑229 (MQ=255)                                                                                                                                                                       tttttCCAATAATTTAAAGACCAAATTCACGCCGACACTGAAATTTGAAGCAAACACGATAGCGGTCTTTTCGGCTGCCGATTTAATTGCCGCTTTGCCGGCGTCATCAAAACCCGTGGTGCCGATAACGATTTTTTTATGGTTGGCAACACAAAATGCCAAATGTTCCAATGTGCCTT‑CCGGGCGGGTGAAATCAATCAGTAAATCGAAACGATCTTTCACCGCGTTTAAGTCATCGCCGACCTTAATGc                                                                    <  1:105239/251‑1 (MQ=255)                                                                                                                                                                        ttttCCAATAATTTAAAGACCAAATTCACGCCGACACTGAAATTTGAAGCAAACACGATAGCGGTCTTTTCGGCTGCCGATTTAATTGCCGCTTTGCCGGCGTCATCAAAACCCGTGGTGCCGATAACGATTTTTTTATGGTTGGCAACACAAAATGCCAAATGTTCCAATGTGCCTT‑CCGGGCGGGTGAaatc                                                                                                                            <  1:401606/194‑1 (MQ=255)                                                                                                                                                                          ttCCAATAATTTAAAGACCAAATTCACGCCGACACTGAAATTTGAAGCAAACACGATAGCGGTCTTTTCGGCTGCCGATTTAATTGCCGCTTTGCCGGCGTCATCAAAACCCGTGGTGCCGATAACGATTTTTTTATGGTTGGCAACACAAAATGCCAAATGTTCCaa                                                                                                                                                     >  1:389628/1‑168 (MQ=255)                                                                                                                                                                                 aatTTAAAGACCAAATTCACGCCGACACTGAAATTTGAAGCAAACACGATAGCGGTCTTTTCGGCTGCCGATTTAATTGCCGCTTTGCCGGCGTCATCAAAACCCGTGGTGCCGATAACGATTTTTTTATGGTTGGCAACACAAAATGCCAAATGTTCCAATGTGCCTT‑CCGGGCGGGTGAAATCAATCAGTAAATc                                                                                                                <  1:22859/197‑1 (MQ=255)                                                                                                                                                                                  atTTAAAGACCAAATTCACGCCGACACTGAAATTTGAAGCAAACACGATAGCGGTCTTTTCGGCTGCCGATTTAATTGCCGCTTTGCCGGCGTCATCAAAACCCGTGGTGCCGATAACGATTTTTTTATGGTTGGCAACACAAAATGCCAAATGTTCCAATGTGCCTT‑CCGGGCGGGTGAAATCAATCAGTAAATCGAAACGATCTTTCACCGCGTTTAAGTCATCGCCGACCTTAATGCCTAAGTTGCCg                                                         <  1:577589/251‑1 (MQ=255)                                                                                                                                                                                     tAAAGACCAAATTCACGCCGACGCTGAAATTTGAAGCAAACACGATAGCGGTCTTTTCGGCTGCCGATTTAATTGCCGCTTTGCCGGCGTCATCAAAACCCGTGGTGCCGATAACGATTTTTTTATGGTTGGCAACACAAAATGCCAAATGTTCCAATGTGCCTT‑CCGGGCGGGTGAAATCAATCAGTAAATCGAAACGATCTTTCACCGCGTTTAAGTCATCGCCGACCTTAAGGCCTAAGTTGCCGAtc                                                      <  1:282920/251‑2 (MQ=255)                                                                                                                                                                                       aaGACCAAATTCACGCCGACACTGAAATTTGAAGCAAACACGATAGCGGTCTTTTCGGCTGCCGATTTAATTGCCGCTTTGTCGGCGTCATCAAAACCCGTGGTGCCGATAACGATTTTTTTATGGTTGGCAACACAAAATGCCAAATGTTCCAATGTGCCTT‑CCGGGCGGGTGAAATCAATCAGTAAATCGAAACGATCTTTCACCGCGTTTAAGTCATCGCCGACCTTAATGCCTAAGTTGCCGAtccc                                                    <  1:443927/251‑4 (MQ=255)                                                                                                                                                                                           ccAAATTCACGCCGACACTGAAATTTGAAGCAAACACGATAGCGGTCTTTTCGGCTGCCGATTTAATTGCCGCTTTGCCGGCGTCATCAAAACCCGTGGTGCCGATAACGATTTTTTTATGGTTGGCAACACAAAATGCCAAATGTTCCAATGTGCCTT‑CCGGGCGGGTGAAATCAATCAGTAAATCGAAACGATCTTTCACCGCGTTTAAGTCATCGCCGACCTTAATGCCTAAGTTGCCGATCCCCGcc                                                <  1:383781/251‑1 (MQ=255)                                                                                                                                                                                                                       aGCAAACACGATAGCGGTCTTTTCGGCTGCCGATTTAATTGCCGCTTTGCCGGCGTCATCAAAACCCGTGGTGCCGATAACGATTTTTTTATGGTTGGCAACACAAAATGCCAAATGTTCCAATGTGCCTT‑CCGGGCGGGTGAAATCAATCAGTAAATCGAAACGATCTTTCACCGCGTTTAAGTCATCGCCGACCTTAATGCCTAAGTTGCCGATCCCCGCCAATTCGCCGGCATCGGCGCCCACCAATg                    >  1:152581/1‑251 (MQ=255)                                                                                                                                                                                                                         cAAACACGATAGCGGTCTTTTCGGCTGCCGATTTAATTGCCGCTTTGCCGGCGTCATCAAAACCCGTGGTGCCGATAACGATTTTTTTATGGTTGGCAACACAAAATGCCAAATGTTCCAATGTGCCTT‑CCGGGCGGGTGAGATCAATCAGTAAATCGAAACGATCTTTCACCGCGTTTAAGTCATCGCCGACCTTAATGCCTAAGTTGCCGATCCCCGCCAATTCGCCGGCATCGGCGCCCACCAATGa                   <  1:610461/250‑1 (MQ=255)                                                                                                                                                                                                                          aaaCACGATAGCGGTCTTTTCGGCTGCCGATTTAATTGCCGCTTTGCCGGCGTCATCAAAACCCGTGGTGCCGATAACGATTTTTTTATGGTTGGCAACACAAAATGCCAAATGTTCCAATGTGCCTT‑CCGGGCGGGTGAAATCAATCAGTAAATCGAAACGATCTTTCACCGCGTTTAAGTCATCGCCGACCTTAATGCCTAAGTTGCCGATCCCCGCCAATTCGCCGGCATCGGCGCCCACCAATGAgg                 >  1:568871/1‑251 (MQ=255)                                                                                                                                                                                                                          aaaCACGATAGCGGTCTTTTCGGCTGCCGATTTAATTGCCGCTTTGCCGGCGTCATCAAAACCCGTGGTGCCGATAACGATTTTTTTATGGTTGGCAACACAAAATGCCAAATGTTCCAATGTGCCTT‑CCGGGCGGGTGAAATCAATCAGTAAATCGAAACGATCTTTCACCGCGTTTAAGTCATCGCCGACCTTAATGCCTAAGTTGCCGATCCCCGCCAATTCGCCGGCATCGGCGCCCACCAATGAgg                 >  1:689798/1‑251 (MQ=255)                                                                                                                                                                                                                            acacGATAGCGGTCTTTTCGGCTGCCGATTTAATTGCCGCTTTGCCGGCGTCATCAAAACCCGTGGTGCCGATAACGATTTTTTTATGGTTGGCAACACAAAATGCCAAATGTTCCAATGTGCCTT‑CCGGGCGGGTGAAATCAATCAGTAAATCGAAACGATCTTTCACCGCGTTTAAGTCATCGCCGACCTTAATGCCTAAGTTGCCGATCCCCGCCAATTCGCCGGCATCGGCGCCCACCAATGAgg                 <  1:212110/249‑1 (MQ=255)                                                                                                                                                                                                                             cacGATAGCGGTCTTTTCGGCTGCCGATTTAATTGCCGCTTTGCCGGCGTCATCAAAACCCGTGGTGCCGATAACGATTTTTTTATGGTTGGCAACACAAAATGCCAAATGTTCCaa                                                                                                                                                     >  1:622377/1‑117 (MQ=255)                                                                                                                                                                                                                             cacGATAGCGGTCTTTTCGGCTGCCGATTTAATTGCCGCTTTGCCGGCGTCATCAAAACCCGTGGTGCCGATAACGATTTTTTTATGGTTGGCAACACAAAATGCCAAATGTTCCAATGTGCCTT‑CCGGGCGGGTGAAATCAATCAGTAAATCGAAACGATCTTTCACCGCGTTTAAGTCATCGCCGACCTTAATGCCTAAGTTGCCGATCCCCGCCAATTCGCCGGCATCGGCGCCCACCAATGAgg                 <  1:582097/248‑1 (MQ=255)                                                                                                                                                                                                                             cacGATAGCGGTCTTTTCGGCTGCCGATTTAATTGCCGCTTTGCCGGCATCATCAAAACCCGTGGTGCCGATAACGATTTTTTTACGATTGGTGACACAAAATGCCAAGTGTTCCAATGTGCCTTCCCGGGCGGGTGAAATCAATCAGCAAATCGAAACGATCTTTCGCCGCGTTTAAGTCATCGCCGACCTTAATGCCTAAGTTGCCGATCCCTGCCAATTCGCCGGCATCGGCGCCCACCAATGAGGaa               >  1:66080/1‑251 (MQ=255)                                                                                                                                                                                                                              ccGATAGCGGTCTTTTCGGCTGCCGATTTAATTGCCGCTTTGCCGGCGTCATCAAAACCCGTGGTGCCGATAACGATTTTTTTATGGTTGGCAACACAAAATGCCAAATGTTCCAATGTGCCTT‑CCGGGCGGGTGAAATCAATCAGTAAATCGAAACGATCTTTCACCGCTTTTAAGTCATCGCCGACCTTAATGCCTAAGTTGCCGATCCCCGCCAATTCGCCGGCATCGGCGCCCACCAATGAGGAAcc             <  1:519382/250‑1 (MQ=255)                                                                                                                                                                                                                              ccGATAGCGGTCTTTTCGGCTGCCGATTTAATTGCCGCTTTGCCGGCGTCATCAAAACCCGTGGTGCCGATAACGATTTTTTTATGGTTGGCAACACAAAATGCCAAATGTTCCAATGTGCCTT‑CCGGGCGGGTGAAATCAATCAGTAAATCGAAACGATCTTTCACCGCGTTTAAGTCATCGCCGACCTTAATGCCTAAGTTGCCGATCCCCGCCAATTCGCCGGCATCGGCGCCCACCAATGAGGAAcc             <  1:837788/250‑1 (MQ=255)                                                                                                                                                                                                                                  tAGCGGTCTTTTCGGCTGCCGATTTAATTGCCGCTTTGCCGGCGTCATCAAAACCCGTGGTGCCGATAACGATTTTTTTATGGTTGGCAACACAAAATGCCAAATGTTCCAATGTGCCTT‑CCGGGCGGGTGAAATCAATCAGTAAATCGAAACGATCTTTCTCCGCGTTTAAGTCATCGCCGACCTTAATGCCTAAGTTGCCGATCCCCGCCAATTCGCCGGCATCGGCGCCCACCAATGAGGAACCTTTg         <  1:261109/251‑1 (MQ=255)                                                                                                                                                                                                                                         cTTTTCGGCTGCCGATTTAATTGCCGCTTTGCCGGCGTCATCAAAACCCGTGGTGCCGATAACGATTTTTTTATGGTTGGCAACACAAAATGCCAAATGTTCCAATGTGCCTT‑CCGGGCGGGTGAAATCAATCAGTAAATCGAAACGATCTTTCACCGCGTTTAAGTCATCGCCGACCTTAATGCCTAAGTTGCCGATCCCCGCCAATTCGCCGGCATCGGCGCCCACCAATGAGGAACCTTTGCGCTCaa  >  1:596478/1‑251 (MQ=255)                                                                                                                                                                                                                                         cTTTTCGGCTGCCGATTTAATTGCCGCTTTGCCGGCGTCATCAAAACCCGTGGTGCCGATAACGATTTTTTTATGGTTGGCAACACAAAATGCCAAATGTTCCAATGTGCCTT‑CCGGGCGGGTGAAATCAATCAGTAAATCGAAACGATCCTTCACCGCGTTTAAGTCATCGCCGACCTTAATGc                                                                    >  1:631766/1‑185 (MQ=255)                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                TAATACCATCGCGACAAAATACGCCGTGGGTTTTCAGATCCCGTCCGAGGGTTTTGGCAATATGTTCGCCCATGGAAAGCGCGGTACCGGACGGCGCATCGACTTTATAGCGGTGGTGCGCTTCGATGATTTCGATGTCGCAATAATCGCCCATCACCTTGGCGGCTTTTTCCAATAATTTAAAGACCAAATTCACGCCGACACTGAAATTTGAAGCAAACACGATAGCGGTCTTTTCGGCTGCTGATTTAATTGCCGCTTTGCCGGCGTCATCAAAACCCGTGGTGCCGATAATGATTTTTTTATGGTTGGCAACACAAAATGCCAAATGTTCCAATGTGCCTT‑CCGGGCGGGTGAAATCAATCAGTAAATCGAAACGATCTTTCACCGCGTTTAAGTCATCGCCGACCTTAATGCCTAAGTTGCCGATTCCCGCCAATTCGCCGGCATCGGCGCCCACCAATGAGGAACCTTTGCGCTCAA  >  1/1616288‑1616770

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑