Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,613,945	G→A	intergenic (‑169317/‑27819)	unknown ← / → unknown	tRNA‑Met‑CAT/tRNA‑Gly‑GCC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,613,945	0	G	A	100.0%	138.2 / NA	50	intergenic (‑169317/‑27819)	unknown/unknown	tRNA‑Met‑CAT/tRNA‑Gly‑GCC
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (29/21);  total (29/21)

AAAGATAACAAAGATCAATACATAGATATCACTATTTGGGATAAAAATTTATTTATTAGGGGTATTGTAAATTCCTTTCATGAAGATAAAGAACTATTCGAAATATACATTGAAAATGCTGATGTTTATAAAACATCAAAAGGTAAAGCAACAAAAATTTACACAGCTCTTAGACTTTCAATATGTGAGCCATATAAAAACCTTATAATTTCAACTACACAACAACCTTCAAAAGGAGAATAGATATGAGATATAACCAAAACCATGAAGCCATTGTAATGGATGGTATAACATTAAGAACCACAAATGAAAGATTATCCACAGAAAACTATAAATTAAAGTCAGATGATTTTAATATAAAGGTTAGGGAACAACAGTCTGCTCCAGTAAAACCCACAACTCCTCCTGTTAGTCATAAATAGTTAAGTTAAATAGGTAAATACTATGGCATACAC  >  1/1613710‑1614164                                                                                                                                                                                                                                            |                                                                                                                                                                                                                            aaaGATAACAAAGATCAATACATAGATATCACTATTTGGGATAAAAATTTATTTATTAGGGGTATTGTAAATTCCTTTCATGAAGATAAAGAACTATTCGAAATATACATTGAAAATGCTGATGTTTATAAAACATCAAAAGGTAAAGCAACAAAAATTTACACAGCTCTTAGACTTTCAATATGTGAGCCATATAAAAACCTTATAATTTCAACTACACAACAACCTTCAAAAGAAGAATAGATATgaga                                                                                                                                                                                                              <  1:180851/251‑1 (MQ=255)   aGATAACAAAGATCAATACATAGATATCACTATTTGGGATAAAAATTTATTTATTAGGGGTATTGTAAATTCCTTTCATGAAGATAAAGAACTATTCGAAATATACATTGAAAATGCTGATGTTTATAAAACATCAAAAGGTAAAGCAACAAAAATTTACACAGCTCTTAGACTTTCAATATGTGAGCCATATAAAAACCTTATAATTTCAACTACACAACAACCTTCAAAAGAAGAATAGATATGAGata                                                                                                                                                                                                            >  1:303329/1‑251 (MQ=255)             gATCAATACATAGATATCACTATTTGGGATAAAAATTTATTTATTAGGGGTATTGTAAATTCCTTTCATGAAGATAAAGAACTATTCGAAATATACATTGAAAATGCTGATGTTTATAAAACATCAAAAGGTAAAGCAACAAAAATTTACACAGCTCTTAGACTTTCAATATGTGAGCCATATAAAAACCTTATAATTTCAACTACACAACAACCTTCAAAAGAAGAATAGATATGAGATATAACCaaaa                                                                                                                                                                                                   >  1:68468/1‑250 (MQ=255)                             tCACTATTTGGGATAAAAATTTATTTATTAGGGGTATTGTAAATTCCTTTCATGAAGATAAAGAACTATTCGAAATATACATTGAAAATGCTGATGTTTATAAAACATCAAAAGGTAAAGCAACAAAAATTTACACAGCTCTTAGACTTTCAATATGTGAGCCATATAAAAACCTTATAATTTCAACTACACAACAACCTTCAAAAGAAGAATAGATATGAGATATAACCAAAACCATGAAGCCATTGTaa                                                                                                                                                                                  >  1:43949/1‑251 (MQ=255)                                            aaaaTTTATTTATTAGGGGTATTGTAAATTCCTTTCATGAAGATAAAGAACTATTCGAAATATACATTGAAAATGCTGATGTTTATAAAACATCAAAAGGTAAAGCAACAAAAATTTACACAGCTCTTAGACTTTCAATATGTGAGCCATATAAAAACCTTATAATTTCAACTACACAACAACCTTCAAAAGAAGAATAGATATGAGATATAACCAAAACCATGAAGCCATTGTAATGGATGGTATAACAt                                                                                                                                                                   <  1:43787/251‑1 (MQ=255)                                             aaaTTTATTTATTAGGGGTATTGTAAATTCCTTTCATGAAGATAAAGAACTATTCGAAATATACATTGAAAATGCTGATGTTTATAAAACATCAAAAGGTAAAGCAACAAAAATTTACACAGCTCTTAGACTTTCAATATGTGAGCCATATAAAAACCTTATAATTTCAACTACACAACAACCTTCAAAAGAAGAATAGATATGAGATATAACCAAAACCATGAAGCCATTGTAATGGATGGTATAACAtt                                                                                                                                                                  >  1:9737/1‑251 (MQ=255)                                                                   gTAAATTCCTTTCATGAAGATAAAGAACTATTCGAAATATACATTGAAAATGCTGATGTTTATAAAACATCAAAAGGTAAAGCAACAAAAATTTACACAGCTCTTAGACTTTCAATATGTGAGCCATATAAAAACCTTATAATTTCAACTACACAACAACCTTCAAAAGAAGAATAGATATGAGATATAACCAAAACCATGAAGCCATTGTAATGGATGGTATAACATTAAGa                                                                                                                                                              >  1:871198/1‑233 (MQ=255)                                                                                   aaGATAAAGAACTATTCGAAATATACATGGAAAATGCTGATGTTTATAAAACATCAAAAGGTAAAGCAACAAAAATTTACACAGCTCTTAGACTTTCAATATGTGAGCCATATAAAAACCTTATAATTTCAACTACACAACAACCTTCAAAAGAAGAATAGATATGAGATATAACCAAAACCATGAAGCCATTGTAATGGATGGTATAACATTAAGAACCACAAATGAAAGATTATCCACAGAAAACtat                                                                                                                             <  1:706830/250‑1 (MQ=255)                                                                                     gATAAAGAACTATTCGAAATATACATTGAAAATGCTGATGTTTATAAAACATCAAAAGGTAAAGCAACAAAAATTTACACAGCTCTTAGACTTTCAATATGTGAGCCATATAAAAACCTTATAATTTCAACTACACAACAACCTTCAAAAGAAGAATAGATATGAGATATAACCAAAACCATGAAGCCATTGTAATGGATGGTATAACAt                                                                                                                                                                   <  1:380112/210‑1 (MQ=255)                                                                                           gAACTATTCGAAATATACATTGAAAATGCTGATGTTTATAAAACATCAAAAGGTAAAGCAACAAAAATTTACACAGCTCTTAGACTTTCAATATGTGAGCCATATAAAAACCTTATAATTTCAACTACACAACAACCTTCAAAAGAAGAATAGATATGAGATATAACCAAAACCATGAAGCCATTGTAATGGATGGTATAACATTAAGAACCACAAATGAAAGATTATCcac                                                                                                                                       <  1:607453/232‑1 (MQ=255)                                                                                              cTATTCGAAATATACATTGAAAATGCTGATGTTTATAAAACATCAAAAGGTAAAGCAACAAAAATTTACACAGCTCTTAGACTTTCAATATGTGAGCCATATAAAAACCTTATAATTTCAACTACACAACAACCTTCAAAAGAAGAATAGATATGAGATATAAcc                                                                                                                                                                                                       >  1:445931/1‑165 (MQ=255)                                                                                                aTTCGAAATATACATTGAAAATGCTGATGTTTATAAAACATCAAAAGGTAAAGCAACAAAAATTTACACAGCTCTTAGACTTTCAATATGTGAGCCATATAAAAACCTTATAATTTCAACTACACAACAACCTTCAAAAGAAGAATAGATATGAGATATAACCAAAACCATGAAGCCATTGTAatggat                                                                                                                                                                             >  1:668140/1‑189 (MQ=255)                                                                                                aTTCGAAATATACATTGAAAATGCTGATGTTTATAAAACATCAAAAGGTAAAGCAACAAAAATTTACACAGCTCTTAGACTTTCAATATGTGAGCCATATAAAAACCTTATAATTTCAACTACACAACAACCTTCAAAAGAAGAATAGATATGAGATATAACCAAAACCATGAAGCCATTGTAATGGATGGTATAACATTAAGAACCACAAATGAAAGATTATCCACAGAAAACTATAAATTAAAGTCAga                                                                                                               >  1:217125/1‑251 (MQ=255)                                                                                                            cATTGAAAATGCTGATGTTTATAAAACATCAAAAGGTAAAGCAACAAAAATTTACACAGCTCTTAGACTTTCAATATGTGAGCCATATAAAAACCTTATAATTTCAACTACACAACAACCTTCAAAAGAAGAATAGATATGAGATATAACCAAAAc                                                                                                                                                                                                  >  1:424228/1‑156 (MQ=255)                                                                                                                     tGCTGATGTTTATAAAACATCAAAAGGTAAAGCAACAAAAATTTACACAGCTCTTAGACTTTCAATATGTGAGCCATATAAAAACCTTATAATTTCAACTACACAACAACCTTCAAAAGAAGAATAGATATGAGATATAACCAAAACCATGAAGCCATTGTAATGGATGGTATAACATTAAGAACCACAAATGaaa                                                                                                                                                 <  1:258904/196‑1 (MQ=255)                                                                                                                            gTTTATAAAACATCAAAAGGTAAAGCAACAAAAATTTACACAGCTCTTAGACTTTCAATATGTGAGCCATATAAAAACCTTATAATTTCAACTACACAACAACCTTCAAAAGAAGAATAGATATGAGATATAACCAAAACCATGAAGCCATTGTAATGGATGGTATAACATTAAGa                                                                                                                                                              >  1:769756/1‑176 (MQ=255)                                                                                                                             tttATAAAACATCAAAAGGTAAAGCAACAAAAATTTACACAGCTCTTAGACTTTCAATATGTGAGCCATATAAAAACCTTATAATTTCAACTACACAACAACCTTCAAAAGAAGAATAGATATGAGATATAACCAAAACCATGAAGCCATTGTAATGGATGGTATAACATTAAGAACCACAAATGAAAGATTATCCACAGAAAACTATAAATTAAAGTCAGATGATTTTAATATAAAGGTTAGGGaacaac                                                                                  <  1:782639/251‑1 (MQ=255)                                                                                                                              ttATAAAACATCAAAAGGTAAAGCAACAAAAATTTACACAGCTCTTAGACTTTCAATATGTGAGCCATATAAAAACCTTATAATTTCAACTACACAACAACCTTCAAAAGAAGAATAGATATGAGat                                                                                                                                                                                                             >  1:121922/1‑127 (MQ=255)                                                                                                                              ttATAAAACATCAAAAGGTAAAGCAACAAAAATTTACACAGCTCTTAGACTTTCAATATGTGAGCCATATAAAAACCTTATAATTTCAACTACACAACAACCTTCAAAAGAAGAATAGATATGAGATATAACCAAAACCATGAAGCCATTGTAATGGATGGTATAACAt                                                                                                                                                                   <  1:776604/169‑1 (MQ=255)                                                                                                                                    aaCATCAAAAGGTAAAGCAACAAAAATTTACACAGCTCTTAGACTTTCAATATGTGAGCCATATAAAAACCTTATAATTTCAACTACACAACAACCTTCAAAAGAAGAATAGATATGAGATATAAcc                                                                                                                                                                                                       >  1:182752/1‑127 (MQ=255)                                                                                                                                       aTCAAAAGGTAAAGCAACAAAAATTTACACAGCTCTTAGACTTTCAATATGTGAGCCATATAAAAACCTTATAATTTCAACTACACAACAACCTTCAAAAGAAGAATAGATATGAGATATAACCAAAACCATGAAGCCATTGTAATGGATGGTCTAACATTAAGAACCACAAATGAAAGATTATCCACAGAAAACTATAAATTAAAGTCAGATGATTTTAATATAAAGGTTAGGGAACAACAGTCTGCTc                                                                         >  1:819104/1‑250 (MQ=255)                                                                                                                                        tCAAAAGGTAAAGCAACAAAAATTTACACAGCTCTTAGACTTTCAATATGTGAGCCATATAAAAACCTTATAATTTCAACTACACAACAACCTTCAAAAGAAGAATAGATATGAGATATAACCAAAACCATGAAGCCATTGTAATGGATGGTATAACAt                                                                                                                                                                   >  1:354336/1‑159 (MQ=255)                                                                                                                                                            aaTTTACACAGCTCTTAGACTTTCAATATGTGAGCCATATAAAAACCTTATAATTTCAACTACACAACAACCTTCAAAAGAAGAATAGATATGAGATATAACCAAAACCATGAAGCCATTGTAATGGATGGTATAACAt                                                                                                                                                                   >  1:659999/1‑139 (MQ=255)                                                                                                                                                                      gCTCTTAGACTTTCAATATGTGAGCCATATAAAAACCTTATAATTTCAACTACACAACAACCTTCAAAAGAAGAATAGATATGAGATATAACCAAAACCATGAAGCCATTGTAatggat                                                                                                                                                                             >  1:241597/1‑119 (MQ=255)                                                                                                                                                                      gCTCTTAGACTTTCAATATGTGAGCCATATAAAAACCTTATAATTTCAACTACACAACAACCTTCAAAAGAAGAATAGATATGAGATATAACCAAAACCATGAAGCCATTGTAATGGATGGTATAACATTAAGAACCACAAATGAAAGATTATCCACAGAAAACtat                                                                                                                             >  1:29157/1‑167 (MQ=255)                                                                                                                                                                       ctctTAGACTTTCAATATGTGAGCCATATAAAAACCTTATAATTTCAACTACACAACAACCTTCAAAAGAAGAATAGATATGAGATATAAcc                                                                                                                                                                                                       >  1:387166/1‑92 (MQ=255)                                                                                                                                                                       ctctTAGACTTTCAATATGTGAGCCATATAAAAACCTTATAATTTCAACTACACAACAACCTTCAAAAGAAGAATAGATATGAGATATAACCAAAACCATGAAGCCATTGTAATGGATGGTATAACATTAAGaa                                                                                                                                                             >  1:537488/1‑134 (MQ=255)                                                                                                                                                                       ctctTAGACTTTCAATATGTGAGCCATATAAAAACCTTATAATTTCAACTACACAACAACCTTCAAAAGAAGAATAGATATGAGATATAACCAAAACCATGAAGCCATTGTAATGGATGGTATAACATTAAGAACCACAAATGAAAGATTATCCACAGAAAACTATAAATTAAAGTCAGATGATTTTAATATAAAGGTTAGGGAACAACAGTCTGCTCCAGTAAAACCCACAACTCCTCCTGTTAGt                                            <  1:725762/247‑1 (MQ=255)                                                                                                                                                                              aCTTTCAATATGTGAGCCATATAAAAACCTTATAATTTCAACTACACAACAACCTTCAAAAGAAGAATAGATATGAGATATAACCAAAACCATGAAGCCATTGTAATGGATGGtat                                                                                                                                                                        >  1:316273/1‑116 (MQ=255)                                                                                                                                                                                 ttCAATATGTGAGCCATATAAAAACCTTATAATTTCAACTACACAACAACCTTCAAAAGAAGAATAGATATGAGATATAACCAAAACCATGAAGCCATTGTAATGGATGGTATAACATTAAGAACCACAAATGAAAGATTATCCACAGAAAACTATAAAtt                                                                                                                        <  1:271991/161‑1 (MQ=255)                                                                                                                                                                                   cAATATGTGAGCCATATAAAAACCTTATAATTTCAACTACACAACAACCTTCAAAAGAAGAATAGATATGAGATATAACCAAAACCATGAAGCCATTGTAATGGATGGTATAACAt                                                                                                                                                                   >  1:444742/1‑116 (MQ=255)                                                                                                                                                                                   cAATATGTGAGCCATATAAAAACCTTATAATTTCAACTACACAACAACCTTCAAAAGAAGAATAGATATGAGATATAACCAAAACCATGAAGCCATTGTAATGGATGGTATAACATTAAGAACCACAAATGAAAGATTATCCACAGAAAACTATAAATTAAAGTCAGATGATTTTAATATAAAgg                                                                                              <  1:589772/185‑1 (MQ=255)                                                                                                                                                                                    aaTATGTGAGCCATATAAAAACCTTATAATTTCAACTACACAACAACCTTCAAAAGAAGAATAGATATGAGATATAACCAAAACCATGAAGCCATTGTAATGGATGGTATAACATTAAGAACCACAAATGAAAGATTATCCACAGAAAACTATAAATTAAAGTCAGATGATTTTAATATAAAGGTTAGGGAACAACAGTCTGCTCCAGTAAAACCCACAACTCCTCCTGTTAGTCATAAATagttaagtta                           <  1:842398/251‑1 (MQ=255)                                                                                                                                                                                           gAGCCATATAAAAACCTTATAATTTCAACTTCACAACAACCTTCAAAAGAAGAATAGata                                                                                                                                                                                                                   <  1:137928/60‑1 (MQ=255)                                                                                                                                                                                                 tataAAAACCTTATAATTTCAACTACACAACAACCTTCAAAAGAAGAATAGATATGAGATATAACCAAAACCATGAAGCCATTGTAATGGATGGTATAACATTAAGAACCACAAATGAAAGATTATCCACAGAAAACTATAAATTAAAGTCAGATGATTTTAATATAAAGGTTAGGGAACAACAGTCTGCTCCAGTAAAACCCACAACTCCTCCTGTTAGTCATAAATAGTTAAGTTAAATAGGTAAATAc              <  1:641380/251‑1 (MQ=255)                                                                                                                                                                                                  ataAAAACCTTATAATTTCAACTACACAACAACCTTCAAAAGAAGAATAGATATGAGATATAACCAAAACCATGAAGCCATTGTAATGGATGGTATAACATTAAGAACCACAAATGAAAGATTATCCACAGAAAACTATAAAtt                                                                                                                        <  1:509054/144‑1 (MQ=255)                                                                                                                                                                                                  ataAAAACCTTATAATTTCAACTACACAACAACCTTCAAAAGAAGAATAGATATGAGATATAACCAAAACCATGAAGCCATTGTAATGGATGGTATAACATTAAGAACCACAAATGAAAGATTATCCACAGAAAACTATAAATTAAAGTCAGATGATTTTAATATAAAGGTTAGGGAACAACAGTCTGCTCCAGTAAAAccc                                                              >  1:900015/1‑202 (MQ=255)                                                                                                                                                                                                  ataAAAACCTTATAATTTCAACTACACAACAACCTTCAAAAGAAGAATAGATATGAGATATAACCAAAACCATGAAGCCATTGTAATGGATGGTATAACATTAAGAACCACAAATGAAAGATTATCCACAGAAAACTATAAATTAAAGTCAGATGATTTTAATATAAAGGTTAGGGAACAACAGTCTGCTCCAGTAAAACCCACAACTCCTCCTGTTAGTCATAAATAGTTAAGTTAAATAGGTAAATa               >  1:511802/1‑249 (MQ=255)                                                                                                                                                                                                  ataAAAACCTTATAATTTCAACTACACAACAACCTTCAAAAGAAGAATAGATATGAGATATAACCAAAACCATGAAGCCATTGTAATGGATGGTATAACATTAAGAACCACAAATGAAAGATTATCCACAGAAAACTATAAATTAAAGTCAGATGATTTTAATATAAAGGTTAGGGAACAACAGTCTGCTCCAGTAAAACCCACAACTCCTCCTGTTAGTCATAAATAGTTAAGTTAAATAGGTAAATa               >  1:196771/1‑249 (MQ=255)                                                                                                                                                                                                         ccTTATAATTTCAACTACACAACAACCTTCAAAAGAAGAATAGATATgaga                                                                                                                                                                                                              >  1:242187/1‑51 (MQ=255)                                                                                                                                                                                                         ccTTATAATTTCAACTACACAACAACCTTCAAAAGAAGAATAGATATGAGATATAACCAAAACCATGAAGCCATTGTAATGGATGGTATAACAt                                                                                                                                                                   >  1:719919/1‑94 (MQ=255)                                                                                                                                                                                                         ccTTATAATTTCAACTACACAACAACCTTCAAAAGAAGAATAGATATGAGATATAACCAAAACCATGAAGCCATTGTAATGGATGGTATAACATTAAGAACCACAAATGAAAGATTATCCACAGAAAACTATAAATTAAAGTCAGATGATTTTAATATAAAGGTTAGGGAACAACAGTCTGCTCCAGTAAAACCCACAACTCCTCCTGTTAGTCATAAATAGTTAAGTTAAATAGGTAAATAc              <  1:271379/243‑1 (MQ=255)                                                                                                                                                                                                             ataATTTGAACTACACAACAACCTTCAAAAGAAGAATAGATATGAGATATAACCAAAACCATGAAGCCATTGTAATGGATGGTATAACATTAAGAACCACAAATGAAAGATTATCCACAGAAAACTATAAATTAAAGTCAGATGATTTTAATATAAAGGTTAGGGAACAACAGTCTGCTCCAGTAAAACCCACAACTCCTCCTGTTAGTCATAAATAGTTAAGTTAAATAGGTAAATACTATGGCATacac  <  1:669615/251‑1 (MQ=255)                                                                                                                                                                                                               aaTTTCAACTACACAACAACCTTCAAAAGAAGAATAGATATGAGATATAACCAAAACCATGAAGCCATTGTAATGGATGGTATAACAt                                                                                                                                                                   <  1:245003/88‑1 (MQ=255)                                                                                                                                                                                                                    cAACTACACAACAACCTTCAAAAGAAGAATAGATATGAGATATAACCAAAACCATGAAGCCATTGTAATGGATGGTATAACATTAAGAACCACAAATGAAAGATTATCCACAGAAAACTATAAATTAAAGTCAGATGATTTTAATATAAAGGTTAGGGa                                                                                       <  1:207855/159‑1 (MQ=255)                                                                                                                                                                                                                    cAACTACACAACAACCTTCAAAAGAAGAATAGATATGAGATATAACCAAAACCATGAAGCCATTGTAATGGATGGTATAACATTAAGAACCACAAATGAAAGATTATCCACAGAAAACTATAAATTAAAGTCAGATGATTTTAATATAAAGGTTAGGGAACAACAGTCTGCTCCAGTAAAACCCACAACTCCTCCTGtt                                               >  1:75198/1‑199 (MQ=255)                                                                                                                                                                                                                         acacAACAACCTTCAAAAGAAGAATAGATATGAGATATAACCAAAACCATGAAGCCATTGTAATGGATGGTATAACATTAAGAACCACAAATGAAAGATTATCCACAGAAAACTATAAATTAAAGTCAGATGATTTTAATATAAAGGTTAGGGAACAACAGTCTGCTCCAGTAAAACCCACAACTCCTCCTGTTAGTCATAAATAGTTAAGTTAAATAGGTAAATACTATgg         <  1:598809/232‑1 (MQ=255)                                                                                                                                                                                                                          cacaACAACCTTCAAAAGAAGAATAGATATGAGATATAACCAAAACCATGAAGCCATTGTAATGGATGGTATAACATTAAGAACCACAAATGAAAGATTATCCACAGAAAACTATAAATTAAAGTCAGATGATTTTAATATAAAGGTTAGGGAACAACAGTCTGCTCCAg                                                                      >  1:527215/1‑170 (MQ=255)                                                                                                                                                                                                                           acaacaACCTTCAAAAGAAGAATAGATATGAGATATAACCAAAACCATGAAGCCATTGTAATGGATGGTATAACATTAAGa                                                                                                                                                              <  1:1993/81‑1 (MQ=255)                                                                                                                                                                                                                              acaacCTTCAAAAGAAGAATAGATATGAGATATAACCAAAACCATGAAGCCATTGTAATGGATGGTATAACATTAAGAACCACAAATGAAAGATTATCCACAGAAAACTATAAATTAAAGTCAGATGATTTTAATATAAAGGTTAGGGAACAACAGTCTGCTCCAGTAAAACCCACAACTCCTCCTGTTAGTCATAAATAGTTAAGTTAAATAGGTAAATACTATgg         >  1:69065/1‑227 (MQ=255)                                                                                                                                                                                                                                            |                                                                                                                                                                                                                            AAAGATAACAAAGATCAATACATAGATATCACTATTTGGGATAAAAATTTATTTATTAGGGGTATTGTAAATTCCTTTCATGAAGATAAAGAACTATTCGAAATATACATTGAAAATGCTGATGTTTATAAAACATCAAAAGGTAAAGCAACAAAAATTTACACAGCTCTTAGACTTTCAATATGTGAGCCATATAAAAACCTTATAATTTCAACTACACAACAACCTTCAAAAGGAGAATAGATATGAGATATAACCAAAACCATGAAGCCATTGTAATGGATGGTATAACATTAAGAACCACAAATGAAAGATTATCCACAGAAAACTATAAATTAAAGTCAGATGATTTTAATATAAAGGTTAGGGAACAACAGTCTGCTCCAGTAAAACCCACAACTCCTCCTGTTAGTCATAAATAGTTAAGTTAAATAGGTAAATACTATGGCATACAC  >  1/1613710‑1614164

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑