Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,606,839	T→C	intergenic (‑162211/‑34925)	unknown ← / → unknown	tRNA‑Met‑CAT/tRNA‑Gly‑GCC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,606,839	0	T	C	100.0%	195.5 / NA	72	intergenic (‑162211/‑34925)	unknown/unknown	tRNA‑Met‑CAT/tRNA‑Gly‑GCC
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (42/30);  total (42/30)

GTCGTCACCAACACGTTCAAACCGGTAGCCAGCGGCGTTACCCGCCCTTCAAAAGATTTGCCCGATACCGTGACATAGTTTTGGCTGCCGATAAAACGGTATTGCAGCCAGAATATGCTCAACGAGATAGCAAGCAAAATCACGCCCAGGGTGCTGGCGGCTAAATAATCATTTTGTGCGCCGACAATATAAAAATAAATCTGCGTTGCCAACACATCAAAATTGCCCCCCAGCACGAAGGGAGTGCTGAAATCCGCCAGCGATTGCACCATCACGATCAAAAACGCATTGGCTAACGCCTGTTTGAGTAGCGGCAATACAATAAAACGGAAACTTTGCCAACGGTTTGCCCCTAACATCACGCCCGCTTCTTCCAGTTTGGGCGACAAGGCTTTTAACGCGCCTTCAATAAGCATAAATGCCATGGGCGTGAGCGCCAGCGTGTGGGAAATTAAAATGCCGTTGAAGCCGTACAGCCA  >  1/1606595‑1607073                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                           gtcgtcACCAACACGTTCAAACCGGTAGCCAGCGGCGTTACCCGCCCTTCAAAAGATTTGCCCGATACCGTGACATAGTTTTGGCTGCCGATAAAACGGTATTGCAGCCAGAATATGCTCAACGAGATAGCAAGCAAAATCACGCCCAGGGTGCTGGCGGCTAAATAATCATTTTGTGCGCCGACAATATAAAAATAAATCTGCGTTGCCAACACATCAAAATTGCCCCCCAGCACGAAGGGAGCGCTGaa                                                                                                                                                                                                                                      >  1:627067/1‑251 (MQ=255)    gtcACCAACACGTTCAAACCGGTAGCCAGCGGCGTTACCCGCCCTTCAAAAGATTTGCCCGATACCGTGACATAGTTTTGGCTGCCGATAAAACGGTATTGCAGCCAGAATATGCTCAACGAGATAGCAAGCAAAATCACGCCCAGGGTGCTGGCGGCTAAATAATCATTTTGTGCGCCGACAATATAAAAATAAATCTGCGTTGCCAACACATCAAAATTGCCCCCCAGCACGAAGGGAGCGCTGAAAt                                                                                                                                                                                                                                    >  1:268240/1‑250 (MQ=255)        ccAACACGTTCAAACCGGTAGCCAGCGGCGTTACCCGCCCTTCAAAAGATTTGCCCGATACCGTGACATAGTTTTGGCTGCCGATAAAACGGTATTGCAGCCAGAATATGCTCAACGAGATAGCAAGCAAAATCACGCCCAGGGTGCTGGCGGCTAAATAATCATTTTGTGCGCCGACAATATAAAAATAAATCTGCGTTGCCAACACATCAAAATTGCCCCCCAGCACGAAGGGAGCGCTGAAATCCGcc                                                                                                                                                                                                                               <  1:304660/251‑1 (MQ=255)              cGTTCAAACCGGTAGCCAGCGGCGTTACCCGCCCTTCAAAAGATTTGCCCGATACCGTGACATAGTTTTGGCTGCCGATAAAACGGTATTGCAGCCAGAATATGCTCAACGAGATAGCAAGCAAAATCACGCCCAGGGTGCTGGCGGCTAAATAATCATTTTGTGCGCCGACAATATAAAAATAAATCTGCGTTGCCAACACATCAAAATTGCCCCCCAGCACGAAGGGAGCGCTGAAATCCGCCAGCGAt                                                                                                                                                                                                                         >  1:585282/1‑251 (MQ=255)                   aaaCCGGTAGCCAGCGGCGTTACCCGCCCTTCAAAAGATTTGCCCGATACCGTGACATAGTTTTGGCTGCCGATAAAACGGTATTGCAGCCAGAATATGCTCAACGAGATAGCAAGCAAAATCACGCCCAGGGTGCTGGCGGCTAAATAATCATTTTGTGCGCCGACAATATAAAAATAAATCTGCGTTGCCAACACATCAAAATTGCCCCCCAGCACGAAGGGAGCGCTGAAATCCGCCAGCGATTGCAc                                                                                                                                                                                                                    <  1:573337/251‑1 (MQ=255)                    aaCCGGTAGCCAGCGGCGTTACCCGCCCTTCAAAAGATTTGCCCGATACCGTGACATAGTTTTGGCTGCCGATAAAACGGTATTGCAGCCAGAATATGCTCAACGAGATAGCAAGCAAAATCACGCCCAGGGTGCTGGCGGCTAAATAATCATTTTGTGCGCCGACAATATAAAAATAAATCTGCGTTGCCAACACATCAAAATTGCCCCCCAGCACGAAGGGAGCGCTGAAATCCGCCAGCGATTGCAcc                                                                                                                                                                                                                   <  1:188838/251‑1 (MQ=255)                         gTAGCCAGCGGCGTTACCCGCCCTTCAAAAGATTTGCCCGATACCGTGACATAGTGTTGGCTGCCGATAAAACGGTATTGCAGCCAGAATATGCTCAACGAGATAGCAAGCAAAATCACGCCCAGGGTGCTGGCGGCTAAATAATCATGTTGTGCGCCGACAATATAAAAATAAATCTGCGTTGCCAACACATCAAAATTGCCCCCCAGCACGAAGGGAGCGCTGAAATCCGCCAGCGATTGCACCATCa                                                                                                                                                                                                               <  1:319765/250‑1 (MQ=255)                                   gcgTTACCCGCCCTTCAAAAGATTTGCCCGATACCGTGACATAGTTTTGGCTGCCGATAAAACGGTATTGCAGCCAGAATATGCTCAACGAGATAGCAAGCAAAATCACGCCCAGGGTGCTGGCGGCTAAATAATCATTTTGTGCGCCGACAATATAAAAATAAATCTGCGTTGCCAACACATCAAAATTGCCCCCCAGCACGAAGGGAGCGCTGAAATCCGCCAGCGATTGCACCATCACGATCaaaaa                                                                                                                                                                                                     >  1:283273/1‑250 (MQ=255)                                      ttACCCGCCCTTCAAAAGATTTGCCCGATACCGTGACATAGTTTTGGCTGCCGATAAAACGGTATTGCAGCCAGAATATGCTCAACGAGATAGCAAGCAAAATCACGCCCAGGGCGCTGGCGGCTAAATAATCATTTTGTGCGCCGACAATATAAAAATAAATCTGCGTTGCCAACACATCAAAATTGCCCCCCAGCACGAAGGGAGCGCTGAAATCCGCCAGCGATTGCACCATCACGATCAAAAACGCa                                                                                                                                                                                                 >  1:218310/1‑251 (MQ=255)                                       tACCCGCCCTTCAAAAGATTTGCCCGATACCGTGACATAGTTTTGGCTGCCGATAAAACGGTATTGCAGCCAGAATATGCTCAACGAGATAGCAAGCAAAATCACGCCCAGGGTGCTGGCGGCTAAATAATCATTTTGTGCGCCGACAATATAAAAATAAATCTGCGTTGCCAACACATCAAAATTGCCCCCCAGCACGAAGGGAGCGCTGAAATCCGCCAGCGATTGCACCATCACGATCAAAAACGCAt                                                                                                                                                                                                >  1:492306/1‑251 (MQ=255)                                       tACCCGCCCTTCAAAAGATTTGCCCGATACCGTGACATAGTTTTGGCTGCCGATAAAACGGTATTGCAGCCAGAATATGCTCAACGAGATAGCAAGCAAAATCACGCCCAGGGTGCTGGCGGCTAAATAATCATTTTGTGCGCCGACAATATAAAAATAAATCTGCGTTGCCAACACATCAAAATTGCCCCCCAGCACGAAGGGAGCGCTGAAATCCGCCAGCGATTGCACCATCACGATCAAAAACGCAt                                                                                                                                                                                                <  1:631355/251‑1 (MQ=255)                                             cccTTCAAAAGATTTGCCCGATACCGTGACATAGTTTTGGCTGCCGATAAAACGGTATTGCAGCCAGAATATGCTCAACGAGATAGCAAGCAAAATCACGCCCAGGGTGCTGGCGGCTAAATAATCATTTTGTGCGCCGACAATATAAAAATAAATCTGCGTTGCCAACACATCAAAATTGCCCCCCAGCACGAAGGGAGCGCTGAAATCCGCCAGCGATTGCACCATCACGATCAAAAACGCATTGGCTa                                                                                                                                                                                          >  1:834969/1‑251 (MQ=255)                                                ttCAAAAGATTTGCCCGATACCGTGACATAGTTTTGGCTGCCGATAAAACGGTATTGCAGCCAGAATATGCTCAACGAGATAGCAAGCAAAATCACGCCCAGGGTGCTGGCGGCTAAATAATCATTTTGTGCGCCGACAATATAAAAATAAATCTGCGTTGCCAACACATCAAAATTGCCCCCCAGCACGAAGGGAGCGCTGAAATCCGCCAGCGATTGCACCATCACGATCAAAAACGCATTGGCTAACg                                                                                                                                                                                       >  1:722066/1‑251 (MQ=255)                                                      aGATTTGCCCGATACCGTGACATAGTTTTGGCTGCCGATAAAACGGTATTGCAGCCAGAATATGCTCAACGAGATAGCAAGCAAAATCACGCCCAGGGTGCTGGCGGCTAAATAATCATTTTGTGCGCCGACAATATAAAAATAAATCTGCGTTGCCAACACATCAAAATTGCCCCCCAGCACGAAGGGAGCGCTGaaa                                                                                                                                                                                                                                     <  1:436945/199‑1 (MQ=255)                                                        aTTTGCCCGATACCGTGACATAGTTTTGGCTGCCGATAAAACGGTATTGCAGCCAGAATATGCTCAACGAGATAGCAAGCAAAATCACGCCCAGGGTGCTGGCGGCTAAATAATCATTTTGTGCGCCGACAATATAAAAATAAATCTGCGTTGCCAACACATCAAAATTGCCCCCCAGCACGAAGGGAGCGCTGAAATCCGCCAGCGATTGCACCATCACGATCAAAAACGCATTGGCTAACGCCTGttt                                                                                                                                                                                <  1:328557/250‑1 (MQ=255)                                                           tGCCCGATACCGTGACATAGTTTTGGCTGCCGATAAAACGGTATTGCAGCCAGAATATGCTCAACGAGATAGCAAGCAAAATCACGCCCAGGGTGCTGGCGGCTAAATAATCATTTTGTGCGCCGACAATATAAAAATAAATCTGCGTTGCCAACACATCAAAATTGCCCCCCAGCACGAAGGGAGCGCTGAAATCCGCCAGCGATTGCACCATCACGATCAAAAACGCATTGGCTAACGCCTGTTTGAGt                                                                                                                                                                            <  1:499226/251‑1 (MQ=255)                                                           tGCCCGATACCGTGACATAGTTTTGGCTGCCGATAAAACGGTATTGCAGCCAGAATATGCTCAACGAGATAGCAAGCAAAATCACGCCCAGGGTGCTGGCGGCTAAATAATCATTTTGTGCGCCGACAATATAAAAATAAATCTGCGTTGCCAACACATCAAAATTGCCCCCCAGCACGAAGGGAGCGCTGAAATCCGCCAGCGATTGCACCATCACGATCAAAAACGCATTGGCTAACGCCTGTTTGAGt                                                                                                                                                                            <  1:555461/251‑1 (MQ=255)                                                            gCCCGATACCGTGACATAGTTTTGGCTGCCGATAAAACGGTATTGCAGCCAGAATATGCTCAACGAGATAGCAAGCAAAATCACGCCCAGGGTGCTGGCGGCTAAATAATCATTTTGTGCGCCGACAATATAAAAATAAATCTGCGTTGCCAACACATCAAAATTGCCCCCCAGCACGAAGGGAGCGCTGAAATCCGCCAGCGATTGCACCATCACGATc                                                                                                                                                                                                          <  1:24822/220‑1 (MQ=255)                                                             cccGATACCGTGACATAGTTTTGGCTGCCGATAAAACGGTATTGCAGCCAGAATATGCTCAACGAGATAGCAAGCAAAATCACGCCCAGGGTGCTGGCGGCTAAATAATCATTTTGTGCGCCGACAATATAAAAATAAATCTGCGTTGCCAACACATCAAAATTGCCCCCCAGCACGAAGGGAGCGCTGAAATCCGCCAGCGATTGCACCATCACGATCAAAAACGCATTGGCTAACGCCTGTTTGAGTAg                                                                                                                                                                          >  1:547812/1‑251 (MQ=255)                                                                 aTACCGTGACATAGTTTTGGCTGCCGATAAAACGGTATTGCAGCCAGAATATGCTCAACGAGATAGCAAGCAAAATCACGCCCAGGGTGCTGGCGGCTAAATAATCATTTTGTGCGCCGACAATATAAAAATAAATCTGCGTTGCCAACACATCAAAATTGCCCCCCAGCACGAAGGGAGCGCTGAAATCCGCCAGCGATTGCACCATCACGATCAAAAAc                                                                                                                                                                                                    <  1:211309/221‑1 (MQ=255)                                                                 aTACCGTGACATAGTTTTGGCTGCCGATAAAACGGTATTGCAGCCAGAATATGCTCAACGAGATAGCAAGCAAAATCACGCCCAGGGTGCTGGCGGCTAAATAATCATTTTGTGCGCCGACAATATAAAAATAAATCTGCGTTGCCAACACATCAAAATTGCCCCCCAGCACGAAGGGAGCGCTGAAATCCGCCAGCGATTGCACCATCACGATCAAAAACGCATTGGCTAACGCCTGTTTGAGTAGCgg                                                                                                                                                                       <  1:573545/250‑1 (MQ=255)                                                                       tGACATAGTTTTGGCTGCCGATAAAACGGTATTGCAGCCAGAATATGCTCAACGAGATAGCAAGCAAAATCACGCCCAGGGTGCTGGCGGCTAAATAATCATTTTGTGCGCCGACAATATAAAAATAAATCTGCGTTGCCAACACATCAAAATTGCCCCCCAGCACGAAGGGAGCGCTGAAATCCGCCAGCGATTGCACCATCACGATCAAAAACGCATTGGCTAACGCCTGTTTGAGTAGCGGcaatac                                                                                                                                                                 <  1:742594/250‑1 (MQ=255)                                                                         aCATAGTTTTGGCTGCCGATAAAACGGTATTGCAGCCAGAATATGCTCAACGAGATAGCAAGCAAAATCACGCCCAGGGTGCTGGCGGCTAAATAATCATTTTGTGCGCCGACAATATAAAAATAAATCTGCGTTGCCAACACATCAAAATTGCCCCCCAGCACGAAGGGAGCGCTGAAATCCGCCAGCGATTGCACCATCACGATCAAAAACGCATTGGCTAACGCCTGTTTGAGTAGCGGcaatacaat                                                                                                                                                              >  1:867812/1‑251 (MQ=255)                                                                         aCATAGTTTTGGCTGCCGATAAAACGGTATTGCAGCCAGAATATGCTCAACGAGATAGCAAGCAAAATCACGCCCAGGGTGCTGGCGGCTAAATAATCATTTTGTGCGCCGACAATATAAAAATAAATCTGCGTTGCCAACACATCAAAATTGCCCCCCAGCACGAAGGGAGCGCTGAAATCCGCCAGCGATTGCACCATCACGATCAAAAACGCATTGGCTAACGCCTGTTTGAGTAGCGGcaatacaat                                                                                                                                                              >  1:237019/1‑251 (MQ=255)                                                                                            aaaaaCGGTAATGCAGCCAGGATAAGCTCAACGAGGTAGCAAGCAAAAACACGGCCAGGGTGGTGGCCGCTAAATAAACATTTTGTGCCGCGACAATATAAAAAAAAAACTGCGTTGCCAACACAACAAAATTGCCCCCCAGGACGGAGGGAGCGCTGAAATCCGCCAGCGATTGCACCATCACGATCAAAAACGCATTGGCTAACGCCTGTTTGAGTAGCGGCAATACAATAAAACGGAAACTTTGCCaa                                                                                                                                           <  1:174831/250‑1 (MQ=255)                                                                                             aaaaCGGTATTGCAGCCAGAATATGCTCAACGAGATAGCAAGCAAAATCACGCCCAGGGTGCTGGCGGCTAAATAATCATTTTGTGCGCCGACAATATAAAAATAAATCTGCGTTGCCAACACATCAAAATTGCCCCCCAGCACGAAGGGAGCGCTGAAATCCGCCAGCGATTGCACCATCACGATCAAAAACGCATTGGCTAACGCCTGTTTGAGTAGCGGCAATACAATAAAACGGAAACTTTGCCAAc                                                                                                                                          <  1:521903/251‑1 (MQ=255)                                                                                                   gTATTGCAGCCAGAATATGCTCAACGAGATAGCAAGCAAAATCACGCCCAGGGTGCTGGCGGCTAAATAATCATTTTGTGCGCCGACAATATAAAAATAAATCTGCGTTGCCAACACATCAAAATTGCCCCCCAGCACGAAGGGAGCGCTGAAATCCGCCAGCGATTGCACCATCACGATCAAAAACGCATTGGCTAACGCCTGTTTGAGTAGCGGCAATACAATAAAACGGAAACTTTGCCAACGGTTTg                                                                                                                                    >  1:297656/1‑251 (MQ=255)                                                                                                            ccAGAATATGCTCAACGAGATAGCAAGCAAAATCACGCCCAGGGTGCTGGCGGCTAAATAATCATTTTGTGCGCCGACAATATAAAAATAAATCTGCGTTGCCAACACATCAAAATTGCCCCCCAGCACGAAGGGAGCGCTGAAATCCGCCAGCGATTGCACCATCACGATCAAAAACGCATTGGCTAACGCCTGTTTGAGTAGCGGCAATACAATAAAACGGAAACTTTGCCAACGGTTTGCCCCTAAc                                                                                                                            <  1:794892/250‑1 (MQ=255)                                                                                                               gAATATGCTCAACGAGATAGCAAGCAAAATCACGCCCAGGGTGCTGGCGGCTAAATAATCATTTTGTGCGCCGACAATATAAAAATAAATCTGCGTTGCCAACACATCAAAATTGCCCCCCAGCACGAAGGGAGCGCTGAAATCCGCCAGCGATTGCACCATCa                                                                                                                                                                                                               >  1:527871/1‑164 (MQ=255)                                                                                                               gAATATGCTCAACGAGATAGCAAGCAAAATCACGCCCAGGGTGCTGGCGGCTAAATAATCATTTTGTGCGCCGACAATATAAAAATAAATCTGCGTTGCCAACACATCAAAATTGCCCCCCAGCACGAAGGGAGCGCTGAAATCCGCCAGCGATTGCACCATCa                                                                                                                                                                                                               <  1:478122/164‑1 (MQ=255)                                                                                                               gAATATGCTCAACGAGATAGCAAGCAAAATCACGCCCAGGGTGCTGGCGGCTAAATAATCATTTTGTGCGCCGACAATATAAAAATAAATCTGCGTTGCCAACACATCAAAATTGCCCCCCAGCACGAAGGGAGCGCTGAAATCCGCCAGCGATTGCACCATCACGATCAAAAACGCATTGGCTAACGCCTGTTTGAGTAGCGGCAATACAATAAAACGGAAACTTTGCCAACGGTTTGCCCCTAACATCa                                                                                                                        >  1:74990/1‑251 (MQ=255)                                                                                                                      cTCAACGAGATAGCAAGCAAAATCACGCCCAGGGTGCTGGCGGCTAAATAATCATTTTGTGCGCCGACAATATAAAAATAAATCTGCGTTGCCAACACATCAAAATTGCCCCCCAGCACGAAGGGAGCGCTGAAATCCGCCAGCGATTGCACCATCACGATCAAAAACGCATTGGCTAACGCCTGTTTGAGt                                                                                                                                                                            >  1:728772/1‑192 (MQ=255)                                                                                                                        cAACGAGATAGCAAGCAAAATCACGCCCAGGGTGCTGGCGGCTAAATAATCATTTTGTGCGCCGACAATATAAAAATAAATCTGCGTTGCCAACGCATCAAAATTGCCCCCCAGCACGAAGGGAGCGCTGAAATCCGCCAGCGATTGCACCATCACGATCAAAAACGCATTGGCTAACGCCTGTTTGAGTAGCGGCAATACAATAAAACGGAAACTTTGCCAACGGTTTGCCCCTAACa                                                                                                                           >  1:432015/1‑239 (MQ=255)                                                                                                                        cAACGAGATAGCAAGCAAAATCACGCCCAGGGTGCTGGCGGCTAAATAATCATTTTGTGCGCCGACAATATAAAAATAAATCTGCGTTGCCAACACATCAAAATTGCCCCCCAGCACGAAGGGAGCGCTGAAATCCGCCAGCGATTGCACCATCACGATCAAAAACGCATTGGCTAACGCCTGTTTGAGTAGCGGCAATACAATAAAACGGAAACTTTGCCAACGGTTTGCCCCTAACATCACGCCCGctt                                                                                                               >  1:473255/1‑251 (MQ=255)                                                                                                                        cAACGAGATAGCAAGCAAAATCACGCCCAGGGTGCTGGCGGCTAAATAATCATTTTGTGCGCCGACAATATAAAAATAAATCTGCGTTGCCAACACATCAAAATTGCCCCCCAGCACGAAGGGAGCGCTGAAATCCGCCAGCGATTGCACCATCACGATCAAAAACGCATTGGCTAACGCCTGTTTGAGTAGCGGCAATACAATAAAACGGAAACTTTGCCAACGGTTTGCCCCTAACATCACGCCCGctt                                                                                                               >  1:887423/1‑251 (MQ=255)                                                                                                                          aCGAGATAGCAAGCAAAATCACGCCCAGGGTGCTGGCGGCTAAATAATCATTTTGTGCGCCGACAATATAAAAATAAATCTGCGTTGCCAACACATCAAAATTGCCCCCCAGCACGAAGGGAGCGCTGAAATCCGCCAGCGATTGCACCATCACGATCAAAAACGCATTGGCTAACGCCTGTTTGAGTAGCGGCAATACAATAAAACGGAAACTTTGCCAACGGTTTGCCCCTAACATCACGCCCGctt                                                                                                               >  1:60045/1‑249 (MQ=255)                                                                                                                             agaTAGCAAGCAAAATCACGCCCAGGGTGCTGGCGGCTAAATAATCATTTTGTGCGCCGACAATATAAAAATAAATCTGCGTTGCCAACACATCAAAATTGCCCCCCAGCACGAAGGGAGCGCTGAAATCCGCCAGCGATTGCACCATCACGATCAAAAACGCATTGGCTAACGcc                                                                                                                                                                                     >  1:858798/1‑176 (MQ=255)                                                                                                                               aTAGCAAGCAAAATCACGCCCAGGGTGCTGGCGGCTAAATAATCATTTTGTGCGCCGACAATATAAAAATAAATCTGCGTTGCCAACACATCAAAATTGCCCCCCAGCACGAAGGGAGCGCTGAAATCCGCCAGCGATTGCACCATCACGATCAAAAACGCATTGGCTACCGCCTGTTTGAGTAGCGGCAATACAATAAAACGGAAACTTTGCCAACGGTTTGCCCCTAACATCACGCCCGCTTCTTCCAg                                                                                                        >  1:445010/1‑251 (MQ=255)                                                                                                                                   caagcaaAATCACGCCCAGGGTGCTGGCGGCTAAATAATCATTTTGTGCGCCGACAATATAAAAATAAATCTGCGTTGCCAACACATCAAAATTGCCCCCCAGCACGAAGGGAGCGCTGAAATCCGCCAGCGATTGCACCATCACGATCAAAAACGCATTGGCTAACGCCTGTTTGAGTAGCGGCAATACAATAAAACGGAAACTTTGCCAACGGTTTGCCCCTAACATCACGCCCGCTTCTTCCAGttt                                                                                                     <  1:53355/250‑1 (MQ=255)                                                                                                                                       caaAATCACGCCCAGGGTGCTGGCGGCTAAATAATCATTTTGTGCGCCGACAATATAAAAATAAATCTGCGTTGCCAACACATCAAAATTGCCCCCCAGCACGAAGGGAGCGCTGAAATCCGCCAGCGATTGCACCATCACGATCAAAAACGCATTGGCTAACGCCTGTTTGAGTAGCGGCAATACAATAAAACGGAAACTTTGCCAACGGTTTGCCCCTAACATCACGCCCGCTTCTTCCAGTTTGGGCg                                                                                                <  1:625863/251‑1 (MQ=255)                                                                                                                                                 cccAGGGTGCTGGCGGCTAAATAATCATTTTGTGCGCCGACAATATAAAAATAAATCTGCGTTGCCAACACATCAAAATTGCCCCCCAGCACGAAGGGAGCGCTGAAATCCGCCAGCGATTGCACCATCACGATCAAAAACGCATTGGCTAACGCCTGTTTGAGt                                                                                                                                                                            >  1:586341/1‑165 (MQ=255)                                                                                                                                                     gggTGCTGGCGGCTAAATAATCATTTTGTGCGCCGACAATATAAAAATAAATCTGCGTTGCCAACACATCAAAATTGCCCCCCAGCACGAAGGGAGCGCTGAAATCCGCCAGCGATTGCACCATCACGATCAAAAACGCATTGGCTAACGCCTGTTTGAGTAGCGGCAATACAATAAAACGGAAACTTTGCCAACGGTTTGCCCCTAACATCACGCCCGCTTCTTCCAGTTTGGGCGACAAGGCTTTTaa                                                                                   >  1:626414/1‑250 (MQ=255)                                                                                                                                                       gTGCTGGCGGCTAAATAATCATTTTGTGCGCCGACAATATAAAAATAAATCTGCGTTGCCAACACATCAAAATTGCCCCCCAGCACGAAGGGAGCGCTGAAATCCGCCAGCGATTGCACCATCACGATCAAAAACGCATTGGCTAACGCCTGTTTGAGTAGCgg                                                                                                                                                                       >  1:802697/1‑164 (MQ=255)                                                                                                                                                                 cTAAATAATCATTTTGTGCGCCGACAATATAAAAATAAATCTGCGTTGCCAACACATCAAAATTGCCCCCCAGCACGAAGGGAGCGCTGAAATCCGCCAGCGATTGCACCATCACGATCAAAAACGCATTGGCTAACGCCTGTTTGAGTAGCGGCAATACAATAAAACGGAAACTTTGCCAACGGTTTGCCCCTAACATCACGCCCGCTTCTTCCAGTTTGGGCGACAAGGCTTTTAACGCGCCTTCAATa                                                                      <  1:139449/251‑1 (MQ=255)                                                                                                                                                                 cTAAATAATCATTTTGTGCGCCGACAATATAAAAATAAATCTGCGTTGCCAACACATCAAAATTGCCCCCCAGCACGAAGGGAGCGCTGAAATCCGCCAGCGATTGCACCATCACGATCAAAAACGCATTGGCTAACGCCTGTTTGAGTAGCGGCAATACAATAAAACGGAAACTTTGCCAACGGTTTGCCCCTAACATCACGCCCGCTTCTTCCAGTTTGGGCGACAAGGCTTTTAACGCGCCTTCAATa                                                                      <  1:801563/251‑1 (MQ=255)                                                                                                                                                                   aaaTAATCATTTTGTGCGCCGACAATATAAAAATAAATCTGCGTTGCCAACACATCAAAATTGCCCCCCAGCACGAAGGGAGCGCTGAAATCCGCCAGCGATTGCACCATCACGATCAAAAACGCATTGGCTAACGCCTGTTTGAGTAGCGGCAATACAATAAAACGGAAACTTTGCCAACGGTTTGCCCCTAACATCACGCCCGCTTCTTCCAGTTTGGGCGACAAGGCTTTTAACGCGCCTTCAATAAg                                                                    >  1:26880/1‑251 (MQ=255)                                                                                                                                                                        atCATTTTGTGCGCCGACAATATAAAAATAAATCTGCGTTGCCAACACATCAAAATTGCCCCCCAGCACGAAGGGAGCGCTGAAATCCGCCAGCGATTGCACCATCACGATCAAAAACGCATTGGCTAACGCCTGTTTGAGTAGCGGCAATACAATAAAACGGAAACTTTGCCAACGGTTTGCCCCTAACATCACGCCCGCTTCTTCCAGTTTGGGCGACAAGGCTTTTAACGCGCCTTCAATAAGCATaa                                                               >  1:242911/1‑251 (MQ=255)                                                                                                                                                                                           atatAAAAATAAATCTGCGTTGCCAACACATCAAAATTGCCCCCCAGCACGAAGGGAGCGCTGAAATCCGCCAGCGATTGCACCATCACGATCAAAAACGCATTGGCTAACGCCTGTTTGAGTAGCGGCAATACAATAAAACGGAAACTTTGCCAACGGTTTGCCCCTAACATCACGCCCGCTTCTTCCAGTTTGGGCGACAAGGCTTTTAACGCGCCTTCAATAAGCATAAATGc                                                           <  1:409088/236‑1 (MQ=255)                                                                                                                                                                                            tataAAAATAAATCTGCGTTGCCAACACATCAAAATTGCCCCCCAGCACGAAGGGAGCGCTGAAATCCGCCAGCGATTGCACCATCACGATCAAAAACGCATTGGCTAACGCCTGTTTGAGTAGCGGCAATACAATAAAACGGAAACTTTGCCAACGGTTTGCCCCTAACATCACGCCCGCTTCTTCCAGTTTGGGCGACAAGGCTTTTAACGCGCCTTCAATAAGCATAAATGCCATGGGCGTGatgtta                                           <  1:523394/251‑6 (MQ=255)                                                                                                                                                                                            tataAAAATAAATCTGCGTTGCCAACACATCAAAATTGCCCCCCAGCACGAAGGGAGCGCTGAAATCCGCCAGCGATTGCACCATCACGATCAAAAACGCATTGGCTAACGCCTGTTTGAGTAGCGGCAATACAATAAAACGGAAACTTTGCCAACGGTTTGCCCCTAACATCACGCCCGCTTCTTCCAGTTTGGGCGACAAGGCTTTTAACGCGCCTTCAATAAGCATAAATGCCATGGGCGTGAgcgc                                            >  1:11247/1‑250 (MQ=255)                                                                                                                                                                                             ataAAAATAAATCTGCGTTGCCAACACATCAAAATTGCCCCCCAGCACGAAGGGAGCGCTGAAATCCGCCAGCGATTGCACCATCACGATCAAAAACGCATTGGCTAACGCCTGttt                                                                                                                                                                                >  1:789178/1‑117 (MQ=255)                                                                                                                                                                                                 aaataaatCTGCGTTGCCAACACATCAAAATTGCCCCCCAGCACGAAGGGAGCGCTGAAATCCGCCAGCg                                                                                                                                                                                                                           >  1:495782/1‑70 (MQ=255)                                                                                                                                                                                                 aaataaatCTGCGTTGCCAACACATCAAAATTGCCCCCCAGCACGAAGGGAGCGCTGAAATCCGCCAGCGATTGCACCATCACGTTCAAAAACGCATTGGCTAACGCCTGTTTGAGTAGCGGCAATACAATAAAACGGAAACTTTGCCAACGGTTTGCCCCTAACATCACGCCCGCTTCTTCCAGTTTGGGCGACAAGGCTTTTAACGCGCCTTCAATAAGCATAAATGCCATGGGCGTGAg                                               >  1:4028/1‑242 (MQ=255)                                                                                                                                                                                                 aaataaatCTGCGTTGCCAACACATCAAAATTGCCCCCCAGCACGAAGGGAGCGCTGAAATCCGCCAGCGATTGCACCATCACGATCAAAAACGCATTGGCTAACGCCTGTTTGAGTAGCGGCAATACAATAAAACGGAAACTTTGCCAACGGTTTGCCCCTAACATCACGCCCGCTTCTTCCAGTTTGGGCGACAAGGCt                                                                                        >  1:321563/1‑201 (MQ=255)                                                                                                                                                                                                 aaataaatCTGCGTTGCCAACACATCAAAATTGCCCCCCAGCACGAAGGGAGCGCTGAAATCCGCCAGCGATTGCACCATCACGATCAAAAACGCATTGGCTAACGCCTGTTTGAGTAGCGGCAATACAATAAAACGGAAACTTTGCCAACGGTTTGCCCCTAACATCACGCCCGCTTCTTCCAGTTTGGGCGACAAGGCTTTTAAc                                                                                  >  1:656034/1‑207 (MQ=255)                                                                                                                                                                                                 aaataaatCTGCGTTGCCAACACATCAAAATTGCCCCCCAGCACGAAGGGAGCGCTGAAATCCGCCAGCGATTGCACCATCACGATCAAAAACGCATTGGCTAACGCCTGTTTGAGTAGCGGCAATACAATAAAACGGAAACTTTGCCAACGGTTTGCCCCTAACATCACGCCCGCTTCTTCCAGTTTGGGCGACAAGGCTTTTAACGCGCCTTCAATAAGCATAAATGCCATggg                                                     <  1:324689/236‑1 (MQ=255)                                                                                                                                                                                                 aaataaatCTGCGTTGCCAACACATCAAAATTGCCCCCCAGCACGAAGGGAGCGCTGAAATCCGCCAGCGATTGCACCATCACGATCAAAAACGCATTGGCTAACGCCTGTTTGAGTAGCGGCAATACAATAAAACGGAAACTTTGCCAACGGTTTGCCCCTAACATCACGCCCGCTTCTTCCAGTTTGGGCGACAAGGCTTTTAACGCGCCTTCAATAAGCATAAATGCCATGGGCGTGAGCGCCAGCgt                                      >  1:419589/1‑251 (MQ=255)                                                                                                                                                                                                 aaataaatCTGCGTTGCCAACACATCAAAATTGCCCCCCAGCACGAAGGGAGCGCTGAAATCCGCCAGCGATTGCACCATCACGATCAAAAACGCATTGGCTAACGCCTGTTTGAGTAGCGGCAATACAATAAAACGGAAACTTTGCCAACGGTTTGCCCCTAACATCACGCCCGCTTCTTCCAGTTTGGGCGACAAGGCTTTTAACGCGCCTTCAATAAGCATAAATGCCATGGGCGTGAGCGCCAGCgt                                      >  1:42184/1‑251 (MQ=255)                                                                                                                                                                                                           gCGTTGCCAACACATCAAAATTGCCCCCCAGCACGAAGGGAGCGCTGAAATCCGCCAGCGATTGCACCATCACGATCAAAAACGCATTGGCTAACGCCTGTTTGAGTAGCGGCAATACAATAAAACGGAAACTTTGCCAACGGTTTGCCCCTAACATCACGCCCGCTTCTTCCAGTTTGGGCGACAAGGCTTTTAACGCGCCTTCAATAAGCATAAATGCCATGGGCGTGAGCGCCAGCGTGTGGGAAAtt                            >  1:673546/1‑251 (MQ=255)                                                                                                                                                                                                           gCGTTGCCAACACATCAAAATTGCCCCCCAGCACGAAGGGAGCGCTGAAATCCGCCAGCGATTGCACCATCACGATCAAAAACGCATTGGCTAACGCCTGTTTGAGTAGCGGCAATACAATAAAACGGAAACTTTGCCAACGGTTTGCCCCTAACATCACGCCCGCTTCTTCCAGTTTGGGCGACAAGGCTTTTAACGCGCCTTCAATAAGCATAAATGCCATGGGCGTGAGCGCCAGCGTGTGGGAAAtt                            >  1:471344/1‑251 (MQ=255)                                                                                                                                                                                                                  cAACACATCAAAATTGCCCCCCAGCACGAAGGGAGCGCTGAAATCCGCCAGCGATTGCACCATCACGATCAAAAACGCATTGGCTAACGCCTGTTTGAGTAGCGGCAATACAATAAAACGGAAACTTTGCCAACGGTTTGCCCCTAACATCACGCCCGCTTCTTCCAGTTTGGGCGACAAGGCTTTTAACGCGCCTTCAATAAGCATAAATGCCATGGGCGTGAGCGCCAGCGTGTGGGAAATTAAAATg                      >  1:34695/1‑250 (MQ=255)                                                                                                                                                                                                                  cAACACATCAAAATTGCCCCCCAGCACGAAGGGAGCGCTGAAATCCGCCAGCGATTGCACCATCACGATCAAAAACGCATTGGCTAACGCCTGTTTGAGTAGCGGCAATACAATAAAACGGAAACTTTGCCAACGGTTTGCCCCTAACATCACGCCCGCTTCTTCCAGTTTGGGCGACAAGGCTTTTAACGCGCCTTCAATAAGCATAAATGCCATGGGCGTGAGCGCCAGCGTGTGGGAAATTAAAATGc                     <  1:524859/251‑1 (MQ=255)                                                                                                                                                                                                                       caTCAAAATTGCCCCCCAGCACGAAGGGAGCGCTGAAATCCGCCAGCGATTGCACCATCACGATCAAAAACGCATTGGCTAACGCCTGTTTGAGTAGCGGCAATACAATAAAACGGAAACTTTGCCAACGGTTTGCCCCTAACATCACGCCCGCTTCTTCCAGTTTGGGCGACAAGGCTTTTaa                                                                                   >  1:779443/1‑184 (MQ=255)                                                                                                                                                                                                                       caTCAAAATTGCCCCCCAGCACGAAGGGAGCGCTGAAATCCGCCAGCGATTGCACCATCACGATCAAAAACGCATTGGCTAACGCCTGTTTGAGTAGCGGCAATACAATAAAACGGAAACTTTGCCAACGGTTTGCCCCTAACATCACGCCCGCTTCTTCCAGTTTGGGCGACAAGGCTTTTAAcg                                                                                 >  1:78587/1‑186 (MQ=255)                                                                                                                                                                                                                       caTCAAAATTGCCCCCCAGCACGAAGGGAGCGCTGAAATCCGCCAGCGATTGCACCATCACGATCAAAAACGCATTGGCTAACGCCTGTTTGAGTAGCGGCAATACAATAAAACGGAAACTTTGCCAACGGTTTGCCCCTAACATCACGCCCGCTTCTTCCAGTTTGGGCGACAAGGCTTTTAACGCGCCTTCAATAAGCATAAATGCCATGGGCGTGAGCGCCAGCGTGTGGGAAATTAAAATGCCGTTg                >  1:773440/1‑251 (MQ=255)                                                                                                                                                                                                                       caTCAAAATTGCCCCCCAGCACGAAGGGAGCGCTGAAATCCGCCAGCGATTGCACCATCACGATCAAAAACGCATTGGCTAACGCCTGTTTGAGTAGCGGCAATACAATAAAACGGAAACTTTGCCAACGGTTTGCCCCTAACATCACGCCCGCTTCTTCCAGTTTGGGCGACAAGGCTTTTAACGCGCCTTCAATAAGCATAAATGCCATGGGCGTGAGCGCCAGCGTGTGGGAAATTAAAATGCCGTTg                >  1:671146/1‑251 (MQ=255)                                                                                                                                                                                                                         tCAAAATTGCCCCCCAGCACGAAGGGAGCGCTGAAATCCGCCAGCGATTGCACCATCACGATCAAAAACGCATTGGCTAACGCCTGTTTGAGTAGCGGCAATACAATAAAACGGAAACTTTGCCAACGGTTTGCCCCTAACATCACGCCCGCTTCTTCCAGTTTGGGCGACAAGGCTTTTAACGCGCCTTCAATAAGCATAAATGCCATGGGCGTGAGCGCCAGCGTGTGGGAAATTAAAATGCCGTTGaa              >  1:230385/1‑251 (MQ=255)                                                                                                                                                                                                                           aaaaTTGCCCCCCAGCACGAAGGGAGCGCTGAAATCCGCCAGCGATTGCACCATCACGATCAAAAACGCATTGGCTAACGCCTGTTTGAGTAGCGGCAATACAATAAAACGGAAACTTTGCCAACGGTTTGCCCCTAACATCACGCCCGCTTCTTCCAGTTTGGGCGACAAGGCTTTTAACGCGCCTTCAATAAGCATAAATGCCATGGGCGTGAGCGCCAGCGTGTGGGAAATTAAAATGCCGTTGAAGc            <  1:612259/251‑1 (MQ=255)                                                                                                                                                                                                                                tGCCCCCCAGCACGAAGGGAGCGCTGAAATCCGCCAGCGATTGCACCATCACGATCAAAAACGCATTGGCTAACGCCTGTTTGAGTAGCGGCAATACAATAAAACGGAAACTTTGCCAACGGTTTGCCCCTAACATCACGCCCGCTTCTTCCAGTTTGGGCGACAAGGCTTTTAACGCGCCTTCAATAAGCATAAATGCCATGGGCGTGAGCGCCAGCGTGTGGGAAATTAAAATGCCGTTGAAGCCGTAc       <  1:564254/251‑1 (MQ=255)                                                                                                                                                                                                                                  ccccccAGCACGAAGGGAGCGCTGAAATCCGCCAGCGATTGCACCATCACGATCAAAAACGCATTGGCTAACGCCTGTTTGAGTAGCGGCAATACAATAAAACGGAAACTTTGCCAACGGTTTGCCCCTAACATCACGCCCGCTTCTTCCAGTTTGGGCGACAAGGCTTTTAACGCGCCTTCAATAAGCATAAATGCCATGGGCGTGAGCGCCAGCGTGTGGGAAATTAAAATGCCGTTGAAGCCGTACAg     <  1:629672/251‑1 (MQ=255)                                                                                                                                                                                                                                   cccccAGCACGAAGGGAGCGCTGAAATCCGCCAGCGATTGCACCATCACGATCAAAAACGCATTGGCTAACGCCTGTTTGAGTAGCGGCAATACAATAAAACGGAAACTTTGCCAACGGTTTGCCCCTAACATCACGCCCGCTTCTTCCAGTTTGGGCGACAAGGCTTTTAACGCGCCTTCAATAAGCATAAATGCCATGGGCGTGAGCGCCAGCGTGTGGGAAATTAAAATGCCGTTGAAGCCGTACAGc    <  1:699285/251‑1 (MQ=255)                                                                                                                                                                                                                                     cccAGCACGAAGGGAGCGCTGAAATCCGCCAGCGATTGCACCATCACGATCAAAAACGCATTGGCTAACGCCTGTTTGAGTAGCGGCAATACAATAAAACGGAAACTTTGCCAACGGTTTGCCCCTAACATCACGCCCGCTTCTTCCAGTTTGGGCGACAAGGCTTTTAACGCGCCTTCAATAAGCATAAATGCCATGGGCGTGAGCGCCAGCGTGTGGGAAATTAAAATGCCGTTGAAGCCGTACAGCCa  <  1:433500/251‑1 (MQ=255)                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                           GTCGTCACCAACACGTTCAAACCGGTAGCCAGCGGCGTTACCCGCCCTTCAAAAGATTTGCCCGATACCGTGACATAGTTTTGGCTGCCGATAAAACGGTATTGCAGCCAGAATATGCTCAACGAGATAGCAAGCAAAATCACGCCCAGGGTGCTGGCGGCTAAATAATCATTTTGTGCGCCGACAATATAAAAATAAATCTGCGTTGCCAACACATCAAAATTGCCCCCCAGCACGAAGGGAGTGCTGAAATCCGCCAGCGATTGCACCATCACGATCAAAAACGCATTGGCTAACGCCTGTTTGAGTAGCGGCAATACAATAAAACGGAAACTTTGCCAACGGTTTGCCCCTAACATCACGCCCGCTTCTTCCAGTTTGGGCGACAAGGCTTTTAACGCGCCTTCAATAAGCATAAATGCCATGGGCGTGAGCGCCAGCGTGTGGGAAATTAAAATGCCGTTGAAGCCGTACAGCCA  >  1/1606595‑1607073

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑