Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,590,470	A→G	intergenic (‑145842/‑51294)	unknown ← / → unknown	tRNA‑Met‑CAT/tRNA‑Gly‑GCC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,590,470	0	A	G	100.0%	196.0 / NA	69	intergenic (‑145842/‑51294)	unknown/unknown	tRNA‑Met‑CAT/tRNA‑Gly‑GCC
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (41/28);  total (41/28)

GTATAAATTTTCACCACGTCATATTTTTCAAGAAACATTCTTATTACTCAGCCTCGGCATCGCCACCGCCTCTTTCGCCGAAGTCAATATTGCCAATTACACCGCCAATCTTCCCAAGGGGGCAAATTTGGCATTCATCGCCAAAAACATCAATCAAGATAACATTATCGCCGATTATAACGGACAAACGTTCATGCTCCCCGCCAGCACGCAAAAAGTATTTACCGCTTTGGCGGCAAAATTGACGCTCGGCGATTCATTTCAATTTCAAACCGCACTTTTAACCAACGGCAAAGTACAAAACGGTCAACTCAACGGCGATTTAATTGTGCGTTTTACCGGCGACCCGGATTTAACCAGCGGGCAATTATTCAATTTATTATCGGAATTAAAGAAGCAAAACATTAATAAAATCACCGGCAATCTGATTTTAGATACGTCGGTGTTTATCGGTCACGATCGCGGTTTAGGCTGGATCTGGAATGATC  >  1/1590226‑1590713                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                    gTATAAATTTTCACCACGTCATATTTTTCAAGAAACATTCTTATTACTCAGCCTCGGCATCGCCACCGCCTCTTTAGCCGAAGTCAATATTGCCAATTACACCGCCAATCTTCCCAAGGGGGCAAATTTGGCATTCATCGCCAAAAACATCAATCAAGATAACATTATCGCCGATTATAACGGACAAACGTTCATGCTCCCCGCCAGCACGCAAAAAGTATTTACCGCTTTGGCGGCAAAATTGGCGCTCg                                                                                                                                                                                                                                               >  1:109958/1‑251 (MQ=255)                                gAAACATTCTTATTACTCAGCCTCGGCATCGCCACCGCCTCTTTAGCCGAAGTCAATATTGCCAATTACACCGCCAATCTTCCCAAGGGGGCAAATTTGGCATTCATCGCCAAAAACATCAATCAAGATAACATTATCGCCGATTATAACGGACAAACGTTCATGCTCCCCGCCAGCACGCAAAAAGTATTTACCGCTTTGGCGGCAAAATTGGCGCTCGGCGATTCATTTCAATTTCAAACCg                                                                                                                                                                                                                       >  1:554126/1‑244 (MQ=255)                                      ttCTTATTACTCAGCCTCGGCATCGCCACCGCCTCTTTAGCCGAAGTCAATATTGCCAATTACACCGCCAATCTTCCCAAGGGGGCAAATTTGGCATTCATCGCCAAAAACATCAATCAAGATAACATTATCGCCGATTATAACGGACAAACGTTCATGCTCCCCGCCAGCACGCAAAAAGTATTTACCGCTTTGGCGGCAAAATTGGCGCTCGGCGATTCATTTCAATTTCAAACCGCACTTTTAAcc                                                                                                                                                                                                            >  1:585893/1‑249 (MQ=255)                                       tCTTATTACTCAGCCTCGGCATCGCCACCGCCTCTTTAGCCGAAGTCAATATTGCCAATTACACCGCCAATCTTCCCAAGGGGGCAAATTTGGCATTCATCGCCAAAAACATCAATCAAGATAACATTATCGCCGATTATAACGGACAAACGTTCATGCTCCCCGCCAGCACGCAAAAAGTATTTACCGCTTTGGCGGCAAAATTGGCGCTCGGCGATTCATTTCAATTTCAAACCGCACTTTTAACCaa                                                                                                                                                                                                          >  1:389941/1‑250 (MQ=255)                                         tttttccccccccccgccttcccccccGCCTCTTTAGCCGAAGTCAATATTGCCAATTACCCCGCCAATCTTCCCAAGGGGGCAAATTTGGCATTCATCGCCAAAAACATCAATCAAGATAACATTATCGCCGATTATAACGGACAAACGTTCATGCTCCCCGCCAGCACGCAAAAAGTATTTACCGCTTTGGCGGCAAAATTGGCGCTCGGCGATTCATTTCAATTTCAAACCGCACTTTTAACCAACgg                                                                                                                                                                                                       <  1:703564/226‑1 (MQ=255)                                                   gCCTCGGCATCGCCACCGCCTCTTTAGCCGAAGTCAATATTGCCAATTACACCGCCAATCTTCCCAAGGGGGCAAATTTGGCATTCATCGCCAAAAACATCAATCAAGATAACATTATCGCCGATTATAACGGACAAACGTTCATGCTCCCCGCCAGCACGCAAAAAGTATTTACCGCTTTGGCGGCAAAATTGGCGCTCGGCGATTCatttcaattt                                                                                                                                                                                                                              >  1:80569/1‑218 (MQ=255)                                                      ccgactccccccccGCCTCTTTAGCCGAAGTCAATATTGCCAATTACACCGCCAATCTTCCCAAGGGGGCAAATTTGGCATTCATCGCCAAAAACATCAATCAAGATAACATTATCGCCGATTATAACGGACAAACGTTCATGCTCCCCGCCAGCACGCAAAAAGTATTTACCGCTTTGGCGGCAAAATTGGCGCTCGGCGATTCATTTCAATTTCAAACCGCACTTTTAACCAACGGCAAAGTACAAAAc                                                                                                                                                                                          <  1:373499/239‑1 (MQ=255)                                                                       tctTTAGCCGAAGTCAATATTGCCAATTACACCGCCAATCTTCCCAAGGGGGCAAATTTGGCATTCATCGCCAAAAACATCAATCAAGATAACATTATCGCCGATTATAACGGACAAACGTTCATGCTCCCCGCCAGCACGCAAAAAGTATTTACCGCTTTGGCGGCAAAATTGGCGCTCGGCGATTCatttc                                                                                                                                                                                                                                   >  1:333028/1‑193 (MQ=255)                                                                       tctTTAGCCGAAGTCAATATTGCCAATTACACCGCCAATCTTCCCAAGGGGGCAAATTTGGCATTCATCGCCAAAAACATCAATCAAGATAACATTATCGCCGATTATAACGGACAAACGTTCATGCTCCCCGCCAGCACGCAAAAAGTATTTACCGCTTTGGCGGCAAAATTGGCGCTCGGCGATTCATTTCAATTTCAAACCGCACTTTTAACCAACGGCAAAGTACAAAACGGTCAACTCAACGGCGa                                                                                                                                                                         >  1:836173/1‑251 (MQ=255)                                                                        ctTTAGCCGAAGTAAATATTGCCAATTACACCGCCAATCTTCCCAAGGGGGCAAATTTGGCATTCATCGCCAAAAACATCAATCAAGATAACATTATCGCCGATTATAACGGACAAACGTTCATGCTCCCCGCCAGCACGCAAAAAGTATTTACCGCTTTGGCGGCAAAATTGGCGCTCGGCGATTCATTTCAATTTCAAACCGCACTTTTAACCAACGGCAAAGTACAAAAc                                                                                                                                                                                          <  1:417647/233‑1 (MQ=255)                                                                                gAAGTCAATATTGCCAATTACACCGCCAATCTTCCCAAGGGGGCAAATTTGGCATTCATCGCCAAAAACATCAATCAAGATAACATTATCGCCGATTATAACGGACAAACGTTCATGCTCCCCGCCAGCACGCAAAAAGTATTTACCGCTTTGGCGGCAAAATTGGCGCTCGGCGATTCATTTCAATTTCAAACCGCACTTTTAACCAACGGCAAAGTACAAAACGGTCAACTCAACGGCGATTTAATtgt                                                                                                                                                                >  1:397412/1‑251 (MQ=255)                                                                                  aGTCAATATTGCCAATTACACCGCCAATCTTCCCAAGGGGGCAAATTTGGCATTCATCGCCAAAAACATCAATCAAGATAACATTATCGCCGATTATAACGGACAAACGTTCATGCTCCCCGCCAGCACGCAAAAAGTATTTACCGCTTTGGCGGCAAAATTGGCGCTCGGCGATTCATTTCAATTTCAAACCGCACTTTTAACCAACGGCAAAGTACAAAACGGTCAACTCAACGGCGATTTAATtgtg                                                                                                                                                               >  1:11130/1‑250 (MQ=255)                                                                                                 ttACACCGCCAATCTTCCCAAGGGGGCAAATTTGGCATTCATCGCCAAAAACATCAATCAAGATAACATTATCGCCGATTATAACGGACAAACGTTCATGCTCCCCGCCAGCACGCAAAAAGTATTTACCGCTTTGGCGGCAAAATTGGCGCTCGGCGATTCATTTCAATTTCAAACCGCACTTTTAACCAACGGCAAAGTACAAAACGGTCAACTCAACGGCGATTTAATTGTGCGTTTTACCGGCGAc                                                                                                                                                >  1:287172/1‑250 (MQ=255)                                                                                                      ccGCCAATCTTCCCAAGGGGGCAAATTTGGCATTCATCGCCAAAAACATCAATCAAGATAACATTATCGCCGATTATAACGGACAAACGTTCATGCTCCCCGCCAGCACGCAAAAAGTATTTACCGCTTTGGCGGCAAAATTGGCGCTCGGCGATTCATTTCAATTTCAAACCGCACTTTTAAcc                                                                                                                                                                                                            >  1:660172/1‑185 (MQ=255)                                                                                                      ccGCCAATCTTCCCAAGGGGGCAAATTTGGCATTCATCGCCAAAAACATCAATCAAGATAACATTATCACCGATTATAACGGACAAACGTTCATGCTCCCCGCCAGCACGCAAAAAGTATTTACCGCTTTGGCGGCAAAATTGGCGCTCGGCGATTCATTTCAATTTCAAACCGCACTTTTAACCAACGGCAAAGTACAAAACGGTCAACTCAACGGCGATTTAATTGTGCGTTTTACCGGCGACCCGGAt                                                                                                                                          >  1:712219/1‑251 (MQ=255)                                                                                                       cGCCAATCTTCCCAAGGGGGCAAATTTGGCATTCATCGCCAAAAACATCAATCAAGATAACATTATCGCCGATTATAACGGACAAACGTTCATGCTCCCCGCCAGCACGCAAAAAGTATTTACCGCTTTGGCGGCAAAATTGGCGCTCGGCGATTCATTTCAATTTCAAACCGCACTTTTAACCAACGGCAAAGTACAAAACGGTCAACTCAACGGCGATTTAATTGTGCGTTTTACCGGCGACCCGGAtt                                                                                                                                         <  1:594313/251‑1 (MQ=255)                                                                                                       cGCCAATCTTCCCAAGGGGGCAAATTTGGCATTCATCGCCAAAAACATCAATCAAGATAACATTATCGCCGATTATAACGGACAAACGTTCATGCTCCCCGCCAGCACGCAAAAAGTATTTACCGCTTTGGCGGCAAAATTGGCGCTCGGCGATTCATTTCAATTTCAAACCGCACTTTTAACCAACGGCAAAGTACAAAACGGTCAACTCAACGGCGATTTAATTGTGCGTTTTACCGGCGACCCGGAtt                                                                                                                                         >  1:670457/1‑251 (MQ=255)                                                                                                         ccAATCTTCCCAAGGGGGCAAATTTGGCATTCATCGCCAAAAACATCAATCAAGATAACATTATCGCCGATTATAACGGACAAACGTTCATGCTCCCCGCCAGCACGCAAAAAGTATTTACCGCTTTGGCGGCAAAATTGGCGCTCGGCGATTCATTTCAATTTCAAACCGCACTTTTAACCAACGGCAAAGTACAAAACGGTCAACTCAACGGCGATTTAATTGTGCGTTTTACCGGCGACCCGGATTTa                                                                                                                                       >  1:854171/1‑251 (MQ=255)                                                                                                         ccAATCTTCCCAAGGGGGCAAATTTGGCATTCATCGCCAAAAACATCAATCAAGATAACATTATCGCCGATTATAACGGACAAACGTTCATGCTCCCCGCCAGCACGCAAAAAGTATTTACCGCTTTGGCGGCAAAATTGGCGCTCGGCGATTCATTTCAATTTCAAACCGCACTTTTAACCAACGGCAAAGTACAAAACGGTCAACTCAACGGCGATTTAATTGTGCGTTTTACCGGCGACCCGGATTTa                                                                                                                                       >  1:307998/1‑251 (MQ=255)                                                                                                            aTCTTCCCAAGGGGGCAAATTTGGCATTCATCGCCAAAAACATCAATCAAGATAACATTATCGCCGATTATAACGGACAAACGTTCATGCTCCCCGCCAGCACGCAAAAAGTATTTACCGCTTTGGCGGCAAAATTGGCGCTCGGCGATTCATTTCAATTTCAAACCGCACTTTTAACCAACGGCAAAGTACAAAACGGTCAACTCAACGGCGATTTAATTGTGCGTTTTACCGGCGACCCGGATTTAAcc                                                                                                                                    <  1:780259/251‑1 (MQ=255)                                                                                                            aTCTTCCCAAGGGGGCAAATTTGGCATTCATCGCCAAAAACATCAATCAAGATAACATTATCGCCGATTATAACGGACAAACGTTCATGCTCCCCGCCAGCACGCAAAAAGTATTTACCGCTTTGGCGGCAAAATTGGCGCTCGGCGATTCATTTCAATTTCAAACCGCACTTTTAACCAACGGCAAAGTACAAAACGGTCAACTCAACGGCGATTTAATTGTGCGTTTTACCGGCGACCCGGATTTAAcc                                                                                                                                    <  1:484324/251‑1 (MQ=255)                                                                                                              cTTCCCAAGGGGGCAAATTTGGCATTCATCGCCAAAAACATCAATCAAGATAACATTATCGCCGATTATAACGGACAAACGTTCATGCTCCCCGCCAGCACGCAAAAAGTATTTACCGCTTTGGCGGCAAAATTGGCGCTCGGCGATTCATTTCAATTTCAAACCGCACTTTTAACCAACGGCAAAGTACAAAACGGTCAACTCAACGGCGATTTAATTGTGCGTTTTACCGGCGACCCGGAtt                                                                                                                                         <  1:284538/244‑1 (MQ=255)                                                                                                              cTTCCCAAGGGGGCAAATTTGGCATTCATCGCCAAAAACATCAATCAAGATAACATTATCGCCGATTATAACGGACAAACGTTCATGCTCCCCGCCAGCACGCAAAAAGTATTTACCGCTTTGGCGGCAAAATTGGCGCTCGGCGATTCATTTCAATTTCAAACCGCACTTTTAACCAACGGCAAAGTACAAAACGGTCAACTCAACGGCGATTTAATTGTGCGTTTTACCGGCGACCCGGATTTAAcc                                                                                                                                    <  1:485743/249‑1 (MQ=255)                                                                                                                tCCCAAGGGGGCAAATTTGGCATTCATCGCCAAAAACATCAATCAAGATAACATTATCGCCGATTATAACGGACAAACGTTCATGCTCCCCGCCAGCACGCAAAAAGTATTTACCGCTTTGGCGGCAAAATTGGCGCTCGGCGATTCATTTCAATTTCAAACCGCACTTTTAACCAACGGCAAAGTACAAAACGGTCAACTCAACGGCGATTTAATTGTGCGTTTTACCGGCGACCCGGATTTAACCAGCg                                                                                                                                >  1:362264/1‑251 (MQ=255)                                                                                                                tCCCAAGGGGGCAAATTTGGCATTCATCGCCAAAAACATCAATCAAGATAACATTATCGCCGATTATAACGGACAAACGTTCATGCTCCCCGCCAGCACGCAAAAAGTATTTACCGCTTTGGCGGCAAAATTGGCGCTCGGCGATTCATTTCAATTTCAAACCGCACTTTTAACCAACGGCAAAGTACAAAACGGTCAACTCAACGGCGATTTAATTGTGCGTTTTACCGGCGACCCGGATTTAACCAGCg                                                                                                                                >  1:445595/1‑251 (MQ=255)                                                                                                                      gggggCAAATTTGGCATTCATCGCCAAAAACATCAATCAAGATAACATTATCGCCGATTATACCGGACAAACGTTCATGCTCCCCGCCAGCACGCAAAAAGTATTTACCGCTTTGGCGGCAAAATTGGCGCTCGGCGATTCATTTCAATTTCAAACCGCACTTTTAACCAACGGCAAAGTACAAAACGGTCAACTCAACGGCGATTTAATTGTGCGTTTTACCGGCGACCCGGATTTAAcc                                                                                                                                    <  1:536800/241‑1 (MQ=255)                                                                                                                      gggggCAAATTTGGCATTCATCGCCAAAAACATCAATCAAGATAACATTATCGCCGATTATAACGGACAAACGTTCATGCTCCCCGCCAGCACGCAAAAAGTATTTACCGCTTTGGCGGCAAAATTGGCGCTCGGCGATTCATTTCAATTTCAAACCGCACTTTTAACCAACGGCAAAGTAc                                                                                                                                                                                               <  1:7018/182‑1 (MQ=255)                                                                                                                             aaTTTGGCATTCATCGCCAAAAACATCAATCAAGATAACATTATCGCCGATTATAACGGACAAACGTTCATGCTCCCCGCCAGCACGCAAAAAGTATTTACCGCTTTGGCGGCAAAATTGGCGCTCGGCGATTCATTTCAATTTCAAACCGCACTTTTAACCAACGGCAAAGTACAAAACGGTCAACTCAACGGCGATTTAATTGTGCGTTTTACCGGCGACCCGGATTTAACCAGCGGGCAATTATTCaa                                                                                                                   <  1:680146/251‑1 (MQ=255)                                                                                                                                ttGGCATTCATCGCCAAAAACATCAATCAAGATAACATTATCGCCGATTATAACGGACAAACGTTCATGCTCCCCGCCAGCACGCAAAAAGTATTTACCGCTTTGGCGGCAAAATTGGCGCTCGGCGATTCATTTCAATTTCAAACCGCACTTTTAACCAACGGCAAAGTACAAAACGGTCAACTCAACGGCGATTTAATTGTGCGTTTTACCGGCGACCCGGATTTAACCAGCGGGCAATTATTCAAttt                                                                                                                <  1:801671/251‑1 (MQ=255)                                                                                                                                  ggCATTCATCGCCAAAAACATCAATCAAGATAACATTATCGCCGATTATAACGGACAAACGTTCATGCTCCCCGCCAGCACGCAAAAAGTATTTACCGCTTTGGCGGCAAAATTGGCGCTCGGCGATTCATTTCAATTTAAAACCGCACTTTTAACCAACGGCAAAGTACAAAACGGTCAACTCAACGGCGATTTAATTGTGCGTTTTACCGGCGACCCGGATTTAACCAGCGGGCAATTATTCAATttat                                                                                                              >  1:655680/1‑251 (MQ=255)                                                                                                                                            gCCAAAAACATCAATCAAGATAACATTATCGCCGATTATAACGGACAAACGTTCATGCTCCCCGCCAGCACGCAAAAAGTATTTACCGCTTTGGCGGCAAAATTGGCGCTCGGCGATTCATTTCAATTTCAAACCGCACTTTTAACCAACGGCAAAGTACAAAACGGTCAACTCAACGGCGATTTAATTGTGCGTTTTACCGGCGACCCGGATTTAACCAGCGGGCAATTATTCAATTTATTATCGGAAtt                                                                                                    >  1:345897/1‑251 (MQ=255)                                                                                                                                             ccAAAAACATCAATCAAGATAACATTATCGCCGATTATAACGGACAAACGTTCATGCTCCCCGCCAGCACGCAAAAAGTATTTACCGCTTTGGCGGCAAAATTGGCGCTCGGCGATTCATTTCAATTTCAAACCGCACTTTTAACCAACGGCAAAGTACAAAACGGTCAACTCAACGGCGATTTAATTGTGCGTTTTACCGGCGACCCGGATTTAACCAGCGGGCAATTATTCAATTTATTATCGGAATTa                                                                                                   >  1:623024/1‑251 (MQ=255)                                                                                                                                               aaaaaCATCAATCAAGATAACATTATCGCCGATTATAACGGACAAACGTTCATGCTCCCCGCCAGCACGCAAAAAGTATTTACCGCTTTGGCGGCAAAATTGGCGCTCGGCGATTCATTTCAATTTCAAACCGCACTTTTAACCAACGGCAAAGTACAAAACGGTCAACTCAACGGCGATTTAATtg                                                                                                                                                                 <  1:213315/187‑1 (MQ=255)                                                                                                                                                aaaaCATAAATCAAGATAACATAATCGCCGATTATAACGGACAAACGTTCATGCTCCCCGCCAGCACGCAAAAAGTATTTACCGCTTTGGCGGCAAAATTGGCGCTCGGCGATTCATTTCAATTTCAAACCGCACTTTTAACCAACGGCAAAGTACAAAACGGTCAACTCAACGGCGATTTAATTGTGCGTTTTACCGGCGACCCGGATTTAACCAGCGGGCAATTATTCAATTTATTATCGGAATTAaag                                                                                                <  1:821751/251‑1 (MQ=255)                                                                                                                                                           caaGATAACATTATCGCCGATTATAACGGACAAACGTTCATGCTCCCCGCCAGCACGCAAAAAGTATTTACCGCTTTGGCGGCAAAATTGGCGCTCGGCGATTCATTTCAATTTCAAACCGCACTTTTAACCAACGGCAAAGTACAAAACGGTCAACTCAACGGCGATTTAATTGTGCGTTTTACCGGCGACCCGGATTTAACCAGCGGGCAATTATTCAATTTATTATCGGAATTAAAGAAGCAAAACAt                                                                                     >  1:685824/1‑251 (MQ=255)                                                                                                                                                             aGATAACATTATCGCCGATTATAACGGACAAATGTTCATGCTCCCCGCCAGCACGCAAAAAGTATTTACCGCTTTGGCGGCAAAATTGGCGCTCGGCGATTCATTTCAATTTCAAACCGCACTTTTAACCAACGGCAAAGTACAAAACGGTCAACTCAACGGCGATTTAATTGTGCGTTTTACCGGCGACCCGGATTTAAcc                                                                                                                                    <  1:456173/202‑1 (MQ=255)                                                                                                                                                             aGATAACATTATCGCCGATTATAACGGACAAACGTTCATGCTCCCCGCCAGCACGCAAAAAGTATTTACCGCTTTGGCGGCAAAATTGGCGCTCGGCGATTCATTTCAATTTCAAACCGCACTTTTAACCAACGGCAAAGTACAAAACGGTCAACTCAACGGCGATTTACTTGTGCGTTTTACCGGCGACCCGGATTTAACCAGCGGGCAATTATTCAATTTATTATCGGAATTAAAGAAGCAAAACATta                                                                                   >  1:440691/1‑251 (MQ=255)                                                                                                                                                             aGATAACATTATCGCCGATTATAACGGACAAACGTTCATGCTCCCCGCCAGCACGCAAAAAGTATTTACCGCTTTGGCGGCAAAATTGGCGCTCGGCGATTCATTTCAATTTCAAACCGCACTTTTAACCAACGGCAAAGTACAAAACGGTCAACTCAACGGCGATTTAATTGTGCGTTTTACCGGCGACCCGGATTTAACCAGCGGGCAATTATTCAATTTATTATCGGAATTAaag                                                                                                >  1:749482/1‑238 (MQ=255)                                                                                                                                                             aGATAACATTATCGCCGATTATAACGGACAAACGTTCATGCTCCCCGCCAGCACGCAAAAAGTATTTACCGCTTTGGCGGCAAAATTGGCGCTCGGCGATTCATTTCAATTTCAAACCGCACTTTTAACCAACGGCAAAGTACAAAACGGTCAACTCAACGGCGATTTAATTGTGCGTTTTACCGGCGACCCGGATTTAACCAGCGGGCAATTATTCAATTTATTATCGGAATTAAAGAAGCAAAACATta                                                                                   >  1:759815/1‑251 (MQ=255)                                                                                                                                                              gATAACATTATCGCCGATTATAACGGACAAACGTTCATGCTCCCCGCCAGCACGCAAAAAGTATTTACCGCTTTGGCGGCAAAATTGGCGCTCGGCGATTCATTTCAATTTCAAACCGCACTTTTAACCAACGGCAAAGTACAAAACGGTCAACTCAACGGCGATTTAATTGTGCGTTTTACCGGCGACCCGGATTTAACCAGCGGGCAATTATTCAATTTATTATCGGa                                                                                                       >  1:118173/1‑230 (MQ=255)                                                                                                                                                                tAACATTATCGCCGATTATAACGGACAAACGTTCATGCTCCCCGCCAGCACGCAAAAAGTATTTACCGCTTTGGCGGCAAAATTGGCGCTCGGCGATTCATTTCAATTTCAAACCGCACTTTTAACCAACGGCAAAGTACAAAACGGTCAACTCAACGGCGATTTAATTGTGCGTTTTACCGGCGACCCGGATTTAACCAGCGGGCAATTATTCAATTTATTATCGGAATTAAAGAAGCAAAACATtaata                                                                                >  1:4084/1‑251 (MQ=255)                                                                                                                                                                 aaCATTATCGCCGATTATAACGGACAAACGTTCATGCTCCCCGCCAGCACGCAAAAAGTATTTACCGCTTTGGCGGCAAAATTGGCGCTCGGCGATTCATTTCAATTTCAAACCGCACTTTTAACCAACGGCAAAGTACAAAACGGTCAACTCAACGGCGATTTAATTGTGCGTTTTACCGGCGACCCGGATTTAACCAGCGGGCAATTATTCAATTTATTATCGGAATTAAAGAAGCAAAACATtaataa                                                                               <  1:405677/251‑1 (MQ=255)                                                                                                                                                                   cATTATCGCCGATTGTAACGGACAAACGTTCATGCCCCCCGCCAGCACGCAAAAAGTATTTACCTCTTTGGCGGCAAAATTGGCGCTCGGCGATTCATTTCAATTTCAAACCGCACTTTTAACCAACGGCAAAGTACAAAACGGTCAACTCAACGGCGATTTAATTGTGCGTTTTACCGGCGACCCGGATTTAACCAGCGGGCAATTATTCAATTtattat                                                                                                           <  1:846368/221‑1 (MQ=255)                                                                                                                                                                   cATTATCGCCGATTATAACGGACAAACGTTCATGCTCCCCGCCAGCACGCAAAAAGTATTTACCGCTTTGGCGGCAAAATTGGCGCTCGGCGATTCATTTCAATTTCAAACCGCACTTTTAACCAACGGCAAAGTACAAAACGGTCAACTCAACGGCGATTTAATTGTGCGTTTTACCGGCGACCCGGATTTAACCAGCGGGCAATTATTCAATTTATTATCGGAATTAAAGAAGCAAAACATTAATaaaa                                                                             >  1:748532/1‑251 (MQ=255)                                                                                                                                                                   cATTATCGCCGATTATAACGGACAAACGTTCATGCTCCCCGCCAGCACGCAAAAAGTATTTACCGCTTTGGCGGCAAAATTGGCGCTCGGCGATTCATTTCAATTTCAAACCGCACTTTTAACCAACGGCAAAGTACAAAACGGTCAACTCAACGGCGATTTAATTGTGCGTTTTACCGGCGACCCGGATTTAACCAGCGGGCAATTATTCAATTTATTATCGGAATTAAAGAAGCAAAACATTAATaaaa                                                                             >  1:530109/1‑251 (MQ=255)                                                                                                                                                                   cATTATCGCCGATTATAACGGACAAACGTTCATGCTCCCCGCCAGCACGCAAAAAGTATTTACCGCTTTGGCGGCAAAATTGGCGCTCGGCGATTCATTTCAATTTCAAACCGCACTTTTAACCAACGGCAAAGTACAAAACGGTCAACTCAACGGCGATTTAATTGTGCGTTTTACCGGCGACCCGGATTTAACCAGCGGGCAATTATTCAATTTATTATCGGAATTAAAGAAGCAAAACATTAATaaaa                                                                             >  1:42044/1‑251 (MQ=255)                                                                                                                                                                   cATTATCGCCGATTATAACGGACAAACGTTCATGCTCCCCGCCAGCACGCAAAAAGTATTTACCGCTTTGGCGGCAAAATTGGCGCTCGGCGATTCATTTCAATTTCAAACCGCACTTTTAACCAACGGCAAAGTACAAAACGGTCAACTCAACGGCGATTTAATTGTGCGTTTTACCGGCGACCCGGATTTAACCAGCGGGCAATTATTCAATTTATTATCGGAATTAAAGAAGCAAAACATTAATaaaa                                                                             >  1:77092/1‑251 (MQ=255)                                                                                                                                                                             gATTATAACGGACAAACGTTCATGCTCCCCGCCAGCACGCAAAAAGTATTTACCGCTTTGGCGGCAAAATTGGCGCTCGGCGATTCATTTCAATTTCAAACCGCACTTTTAACCAACGGCAAAGTACAAAACGGTCAACTCAACGGCGATTTAATTGTGCGTTTTACCGGCGACCCGGATTTAACCAGCGGGCAATTATTCAATTTATTATCGGAATTAAAGAAGCAAAACATTAATAAAATCACCGGCaa                                                                   >  1:727466/1‑251 (MQ=255)                                                                                                                                                                              aTTATAACGGACAAACGTTCATGCTCCCCGCCAGCACGCAAAAAGTATTTACCGCTTTGGCGGCAAAATTGGCGCTCGGCGATTCATTTCAATTTCAAACCGCACTTTTAACCAACGGCAAAGTACAAAACGGTCAACTCAACGGCGATTTAATTGTGCGTTTTACCGGCGACCCGGATTTAACCAGCGGGCAATTATTCAATTTATTATCGGAATTAAAGAAGCAAAACATTAATAAAATCACCGGCAAt                                                                  >  1:111044/1‑251 (MQ=255)                                                                                                                                                                                      ggACAAACGTTCATGCTCCCCGCCAGCACGCAAAAAGTATTTACCGCTTTGGCGGCAAAATTGGCGCTCGGCGATTCATTTCAATTTCAAACCGCACTTTTAACCAACGGCAAAGTACAAAACGGTCAACTCAACGGCGATTTAATTGTGCGTTTTACCGGCGACCCGGATTTAACCAGCGGGCAATTATTCAATTTATTATCGGAATTAAAGAAGCAAAACATTAATAAAATCACCGGCAATCTGAtttt                                                          >  1:305649/1‑251 (MQ=255)                                                                                                                                                                                         cAAACGTTCATGCTCCCCGCCAGCACGCAAAAAGTATTTACCGCTTTGGCGGCAAAATTGGCGCTCGGCGATTCATTTCAATTTCAAACCGCACTTTTAACCAACGGCAAAGTACAAAACGGTCAACTCAACGGCGATTTAATTGTGCGTTTTACCGGCGACCCGGATTTAACCAGCGGGCAATTATTCAATTTATTATCGGAATTAAAGAAGCAAAACATTAATAAAATCACCGGCAATCTGATTTTAGa                                                       <  1:271952/251‑1 (MQ=255)                                                                                                                                                                                            aCGTTCATGCTCCCCGCCAGCACGCAAAAAGTATTTACCGCTTTGGCGGCAAAATTGGCGCTCGGCGATTCATTTCAATTTCAAACCGCACTTTTAACCAACGGCAAAGTACAAAACGGTCAACTCAACGGCGATTTAATTGTGCGTTTTACCGGCGACCCGGATTTAACCAGCGGGCAATTATTCAATTTATTATCGGAATTAAAGAAGCAAAACATtaataa                                                                               <  1:562719/224‑1 (MQ=255)                                                                                                                                                                                            aCGTTCATGCTCCCCGCCAGCACGCAAAAAGTATTTACCGCTTTGGCGGCAAAATTGGCGCTCGGCAATTCATTTCAATTTCAAACCGCACTTTTAACCAACGGCAAAGTACAAAACGGTCAACTCAACGGCGATTTAATTGTGCGTTTTAc                                                                                                                                                       >  1:78733/1‑152 (MQ=255)                                                                                                                                                                                             cGTTCATGCTCCCCGCCAGCACGCAAAAAGTATTTACCGCTTTGGCGGCAAAATTGGCGCTCGGCGATTCATTTCAATTTCAAACCGCACTTTTAACCAACGGCAAAGTACAAAACGGTCAACTCAACGGCGATTTAATTGTGCGTTTTACCGGCGACCCGGATTTAACCAGCGGGCAATTATTCAATTtattat                                                                                                           >  1:683424/1‑195 (MQ=255)                                                                                                                                                                                             cGTTCATGCTCCCCGCCAGCACGCAAAAAGTATTTACCGCTTTGGCGGCAAAATTGGCGCTCGGCGATTCATTTCAATTTCAAACCGCACTTTTAACCAACGGCAAAGTACAAAACGGTCAACTCAACGGCGATTTAATTGTGCGTTTTACCGGCGACCCGGATTTAACCAGCGGGCAATTATTCAATTTATTATCGGAATTAAAGAAGCAAAACATTAATAAAATCACCGGCAATCTGATTTTAGATACg                                                   >  1:391064/1‑251 (MQ=255)                                                                                                                                                                                             cGTTCATGCTCCCCGCCAGCACGCAAAAAGTATTTACCGCTTTGGCGGCAAAATTGGCGCTCGGCGATTCATTTCAATTTCAAACCGCACTTTTAACCAACGGCAAAGTACAAAACGGTCAACTCAACGGCGATTTAATTGTGCGTTTTACCGGCGACCCGGATTTAACCAGCGGGCAATTATTCAATTTATTATCGGAATTAAAGAAGCAAAACATTAATAAAATCACCGGCAATCTGATTTTAGATACg                                                   >  1:761160/1‑251 (MQ=255)                                                                                                                                                                                               ttCATGCTCCCCGCCAGCACGCAAAAAGTATTTACCGCTTTGGCGGCAAAATTGGCGCTCGGCGATTCATTTCAATTTCAAACCGCACTTTTAACCAACGGCAAAGt                                                                                                                                                                                                 <  1:283021/107‑1 (MQ=255)                                                                                                                                                                                               ttCATGCTCCCCGCCAGCACGCAAAAAGTATTTACCGCTTTGGCGGCAAAATTGGCGCTCGGCGATTCATTTCAATTTCAAACCGCACTTTTAACCAACGGCAAAGTACAAAACGGTCAACTCAACGGCGATTTAATTGTGCGTTTTACCGGCGACCCGGATTTAACCAGCGGGCAATTATTCAATTTATTATCGGAATTAaag                                                                                                >  1:287332/1‑204 (MQ=255)                                                                                                                                                                                               ttCATGCTCCCCGCCAGCACGCAAAAAGTATTTACCGCTTTGGCGGCAAAATTGGCGCTCGGCGATTCATTTCAATTTCAAACCGCACTTTTAACCAACGGCAAAGTACAAAACGGTCAACTCAACGGCGATTTAATTGTGCGTTTTACCGGCGACCCGGATTTAACCAGCGGGCAATTATTCAATTTATTATCGGAATTAAAGAAGCAAAACATTAATAAAATCACCGGCAATCTGATTTTAGATACGt                                                  >  1:172440/1‑250 (MQ=255)                                                                                                                                                                                                         ccGCCAGCACGCAAAAAGTATTTACCGCTTTGGCGGCAAAATTGGCGCTCGGCGATTCATTTCAATTTCAAACCGCACTTTTAACCAACGGCAAAGTACAAAACGGTCAACTCAACGGCGATTTAATTGTGCGTTTTACCGGCGACCCGGATTTAACCAGCGGGCAATTATTCAATTTATTATCGGAATTAAAGAAGCAAAACATTAATAAAATCACCGGCAATCTGATTTTAGATACGTCGGTGTTTATc                                       <  1:637516/251‑1 (MQ=255)                                                                                                                                                                                                                cACGCAAAAAGTATTTACCGCTTTGGCGGCAAAATTGGCGCTCGGCGATTCATTTCAATTTCAAACCGCACTTTTAACCAACGGCAAAGTACAAAACGGTCAACTCAACGGCGATTTAATTGTGCGTTTTACCGGCGACCCGGATTTAACCAGCGGGCAATTATTCAATTTATTATCGGAATTAAAGAAGCAAAACATTAATAAAATCACCGGCAATCTGATTTTAGATACGTCGGTGTTTATCGGTCa                                  <  1:252329/249‑1 (MQ=255)                                                                                                                                                                                                                    cAAAAAGTATTTACCGCTTTGGCGGCAAAATTGGCGCTCGGCGATTCATTTCAATTTCAAACCGCACTTTTAACCAACGGCAAAGTACAAAACGGTCAACTCAACGGCGATTTAATTGTGCGTTTTACCGGCGACCCGGATTTAACCAGCGGGCAATTATTCAATTTATTATCGGAATTAAAGAAGCAAAACATTAATAAAATCACCGGCAATCTGATTTTAGATACGTCGGTGTTTATCGGTCACGATc                             <  1:842632/250‑1 (MQ=255)                                                                                                                                                                                                                        aaGTATTTACCGCTTTGGCGGCAAAATTGGCGCTCGGCGATTCATTTCAATTTCAAACCGCACTTTTAACCAACGGCAAAGTACAAAACGGTCAACTCAACGGCGATTTAATTGTGCGTTTTACCGGCGACCCGGATTTAACCAGCGGGCAATTATTCAATTTATTATCGGAATTAAAGAAGCAAAACATTAATAAAATCACCGGCAATCTGATTTTAGATACGTCGGTGTTTATCGGTCACGATCGCGGt                        >  1:894041/1‑251 (MQ=255)                                                                                                                                                                                                                          gTATTTACCGCTTTGGCGGCAAAATTGGCGCTCGGCGATTCATTTCAATTTCAAACCGCACTTTTAACCAACGGCAAAGTACAAAACGGTCAACTCAACGGCGATTTAATTGTGCGTTTTACCGGCGACCCGGATTTAAcc                                                                                                                                    <  1:741495/141‑1 (MQ=255)                                                                                                                                                                                                                           tATTTACCGCTTTGGCGGCAAAATTGGCGCTCGGCGATTCATTTCAATTTCAAACCGCACTTTTAACCAACGGCAAAGTACAAAACGGTCAACTCAACGGCGATTTAATTGTGCGTTTTACCGGCGACCCGGATTTAACCAGCGGGCAATTATTCAATTTATTATCGGAATTAAAGAAGCAAAACATTAATAAAATCACCGGCAATCTGATTTTAGATACGTCGGTGTTTATCGGTCACGATCGCGGttt                      <  1:901909/250‑1 (MQ=255)                                                                                                                                                                                                                              ttACCGCTTTGGCGGCAAAATTGGCGCTCGGCGATTCATTTCAATTTCAAACCGCACTTTTAACCAACGGCAAAGTACAAAACGGTCAACTCAACGGCGATTTAATTGTGCGTTTTACCGGCGACCCGGATTTAACCAGCGGGCAATTATTCAATTTATTATCGGAATTAAAGAAGCAAAACATTAATAAAATCACCGGCAATCTGATTTTAGATACGTCGGTGTTTATCGGTCACGATCGCGGTTTAGGc                  <  1:852874/251‑1 (MQ=255)                                                                                                                                                                                                                                          cggcAAAATTGGCGCTCGGCGATTCATTTCAATTTCAAACCGCACTTTTAACCAACGGCAAAGTACAAAACGGTCAACTCAACGGCGATTTAATTGTGCGTTTTACCGGCGACCCGGATTTAACCAGCGGGCAATTATTCAATTTATTATCGGAATTAAAGAAGCAAAACATTAATAAAATCACCGGCAATCTGATTTTAGATACGTCGGTGTTTGTCGGTCACGATCGCGGTTTAGGCTGGATCTGGaa       <  1:117677/250‑1 (MQ=255)                                                                                                                                                                                                                                              aaaaTTGGCGCTCGGCGATTCATTTCAATTTCAAACCGCACTTTTAACCAACGGCAAAGTACAAAACGGTCAACTCAACGGCGATTTAATTGTGCGTTTTACCGGCGACCCGGATTTAACCAGCGGGCAATTATTCAATTTATTATCGGAATTAAAGAAGCAAAACATTAATAAAATCACCGGCAATCTGATTTTAGATACGTCGGTGTTTATCGGTCACGATCGCGGTTTAGGCTGGATCTGGAATGATc  <  1:513674/251‑1 (MQ=255)                                                                                                                                                                                                                                               aaaTTGGCGCTCGGCGATTCATTTCAATTTCAAACCGCACTTTTAACCAACGGCAAAGTACAAAACGGTCAACTCAACGGCGATTTAATTGTGCGTTTTACCGGCGACCCGGATTTAACCAGCGGGCAATTATTCAATTTATTATCGGAATTAaag                                                                                                >  1:135716/1‑156 (MQ=255)                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                    GTATAAATTTTCACCACGTCATATTTTTCAAGAAACATTCTTATTACTCAGCCTCGGCATCGCCACCGCCTCTTTCGCCGAAGTCAATATTGCCAATTACACCGCCAATCTTCCCAAGGGGGCAAATTTGGCATTCATCGCCAAAAACATCAATCAAGATAACATTATCGCCGATTATAACGGACAAACGTTCATGCTCCCCGCCAGCACGCAAAAAGTATTTACCGCTTTGGCGGCAAAATTGACGCTCGGCGATTCATTTCAATTTCAAACCGCACTTTTAACCAACGGCAAAGTACAAAACGGTCAACTCAACGGCGATTTAATTGTGCGTTTTACCGGCGACCCGGATTTAACCAGCGGGCAATTATTCAATTTATTATCGGAATTAAAGAAGCAAAACATTAATAAAATCACCGGCAATCTGATTTTAGATACGTCGGTGTTTATCGGTCACGATCGCGGTTTAGGCTGGATCTGGAATGATC  >  1/1590226‑1590713

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑