Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	26,774	A→C	intergenic (–/+104360)	– / ← unknown	–/tRNA‑Ala‑GGC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	26,774	0	A	C	100.0%	77.8 / NA	30	intergenic (–/+104360)	–/unknown	–/tRNA‑Ala‑GGC
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (18/12);  total (18/12)

GTACTTTGAGATATTTTTGATTTAGAACAAATTTATCTGTCCGTTGCAGATTACAATAATGCGCTTTTTAAAATTATAAATTGCGCGCGGTTAAAAAATAATAGCAAACCACCGCCATCAACGAACACGCCGCCATGGTCAGCAACATTGGCGTTGCGCTGCTCATATTCATAAGGGCAACCAGTG‑TCCCACGCAGGCGCCGGTGCCGAAACGTACACTACCGACCATAGAATTTGCCGAGCAGGCGGCATTCGGGAATTTTTCTAAAATGGACGCCATGGCGTTGGAAGAAATCAGCGAGTTTTGTCCCACAAAAATCGCAATGCCAATTGCAATTGCCCAAAAGCCCAAATTCAGCAACA  >  1/26532‑26893                                                                                                                                                                                                                                                    |                                                                                                                        gTACTTTGAGATATTTTTGATTTAGAACAAATTTATCTGTCCGTTGCAGATTACAATAATGCGCTTTTTAAAATTATAAATTGCGCGCGGTTAAAAAATAATAGCAAACCACCGCCATCAACGAACACGCCGCCATGGTCAGCAACATTGGCGTTGTGCTGCTCATATTCATAAGGGCAACCAGTGATCCCACGCAGGCGCCGGTGCCGAAACGTACACTACCGACCATAGAATTTGCCGAGCCGGCGGCa                                                                                                                  >  1:655250/1‑251 (MQ=255)    cTTTGAGATATTTTTGATTTAGAACAAATTTATCTGTCCGTTGCAGATTACAATAATGCGCTTTTTAAAATTATAAATTGCGCGCGGTTAAAAAATAATAGCAAACCACCGCCATCAACGAACACGCCGCCATGGTCAGCAACATTGGCGTTGTGCTGCTCATATTCATAAGGGCAACCAGTGATCCCACGCAGGCGCCGGTGCCGAAACGTACACTACCGACCATAGAATTTGCCGAGCCGGCGGCAtt                                                                                                                >  1:842539/1‑250 (MQ=255)              tttttGATTTAGAACAAATTTATCTGTCCGTTGCAGATTACAATAATGCGCTTTTTAAAATTATAAATTGCGCGCGGTTAAAAAATAATAGCAAACCACCGCCATCAACGAACACGCCGCCATGGTCAGCAATATTGGCGTTGTGCTGCTCATATTCATAAGGGCAACCAGTGATCCCACGCAGGCGCCGGTGCCGAAACGTACACTACCGACCATAGAATTTGCCGAGCCGGCGGCATTCGGGAAttttt                                                                                                     >  1:55149/1‑251 (MQ=255)                             aaaTTTATCTGTCCGTTGCAGATTACAATAATGCGCTTTTTAAAATTATAAATTGCGCGCGGTTAAAAAATAATAGCAAACCACCGCCATCAACGAACACGCCGCCATGGTCAGCAACATTGGCGTTGTGCTGCTCATATTCATAAGGGCAACCAGTGATCCCACGCAGGCGCCGGTGCCGAAACGTACACTACCGACCATAGAATTTGCCGAGCCGGCGGCATTCGGGAATTTTTCTAAAATGGACGCCa                                                                                      >  1:705535/1‑251 (MQ=255)                              aaTTTATCTGTCCGTTGCAGATTACAATAATGCGCTTTTTAAAATTATAAATTGCGCGCGGTTAAAAAATAATAGCAAACCACCGCCATCAACGAACACGCCGCCATGGTCAGCAACATTGCAGATGTGCTGCTCATATTCATAAGGGCAACCAGTGATCCCACGCAGGCGCCGGTGCCGAAACGTACACTACCGACCATAGAACTTGCCGAGCCGGCAGCATTCGAGAATTCTTCTAAAGTGGACGCCAt                                                                                     >  1:585574/1‑251 (MQ=255)                                       gTCCGTTGCAGATTACAATAATGCGCTTTTTAAAATTATAAATTGCGCGCGGTTAAAAAATAATAGCAAACCACCGCCATCAACGAACACGCCGCCATGGTCAGCAACATTGGCGTTGTGCTGCTCATATTCATAAGGGCAACCAGTGATCCCACGCAGGCGCCGGTGCCGAAACGTACACTACCGACCATAGAATTTGCCGAGCCGGCGGCATTCGGGAATTTTTCTAAAATGGACGCCATGGCGTTGga                                                                            >  1:708083/1‑251 (MQ=255)                                         ccGTTGAAGATTACAATAATGCGCTTTTTAAAATTATAAATTGCGCGCGGTTAAAAAATAATAGCAAACCACCGCCATCAACGAACACGCCGCCATGGTCAGCAACATTGGCGTTGTGCTGCTCATATTCATAAGGGCAACCAGTGATCCCACGCAGGCGCCGGTGCCGAAACGTACACTACCGACCATAGAATTTGCCGAGCCGGCGGCATTCGGGAATTTTTCTAAAATGGACGCCATGGCGTTGgaag                                                                          <  1:582164/251‑1 (MQ=255)                                            ttGCAGATTACAATAATGCGCTTTTTAAAATTATAAATTGCGCGCGGTTAAAAAATAATAGCAAACCACCGCCATCAACGAACACGCCGCCATGGTCAGCAACATTGGCGTTGTGCTGCTCATATTCATAAGGGCAACCAGTGATCCCACGCAGGCGCCGGTGCCGAAACGTACACTACCGACCATAGAATTTGCCGAGCCGGCGGCATTCGGGAATTTTTCTAAAATGGACGCCATGGCGTTGGAAGaaa                                                                       >  1:740735/1‑251 (MQ=255)                                                 gATTACAATAATGCGCTTTTTAAAATTATAAATTGCGCGCGGTTAAAAAATAATAGCAAACCACCGCCATCAACGAACACGCCGCCATGGTCAGCAACATTGGCGTTGTGCTGCTCATATTCATAAGGGCAACCAGTGATCCCACGCAGGCGCCGGTGCCGAAACGTACACTACCGACCATAGAATTTGCCGAGCCGGCGGCATTCGGGAATTTTTCTAAAATGGACGCCAt                                                                                     <  1:612092/232‑1 (MQ=255)                                                   ttACAATAATGCGCTTTTTAAAATTATAAATTGCGCGCGGTTAAAAAATAATAGCAAACCACCGCCATCAACGAACACGCCGCCATGGTCAGCAACATTGGCGTTGTGCTGCTCATATTCATAAGGGCAACCAGTGATCCCACGCAGGCGCCGGTGCCGAAACGTACACTACCGACCATAGAATTTGCCGAGCCGGCGGCATTCGGGAATTTTTCTAAAATGGACGCCATGGCGTTGgaag                                                                          <  1:46054/241‑1 (MQ=255)                                                        ataatGCGCTTTTTAAAATTATAAATTGCGCGCGGTTAAAAAATAATAGCAAACCACCGCCATCAACGAACACGCCGCCATGGTCAGCAACATTGGCGTTGTGCTGCTCATATTCATAAGGGCAACCAGTGATCCCACGCAGGCGCCGGTGCCGAAACGTACACTACCGACCATAGAATTTGCCGAGCCGGCGGCATTCGGGAATTTTTCTAAAATGGACGCCATGGCGTTGGAAGAAATCAGCGAGtttt                                                           >  1:58209/1‑251 (MQ=255)                                                                  ttttAAAATTATAAATTGCGCGCGGTTAAAAAATAATAGCAAACCACCGCCATCAACGAACACGCCGCCATGGTCAGCAACATTGGCGTTGTGCTGCTCATATTCATAAGGGCAACCAGTGATCCCACGCAGGCGCCGGTGCCGAAACGTACACTACCGACCATAGAATTTGCCGAGCCGGCGGCATTCggg                                                                                                            >  1:590240/1‑192 (MQ=255)                                                                         aTTATAAATTGCGCGCGGTTAAAAAATAATAGCAAACCACCGCCATCAACGAACACGCCGCCATGGTCAGCAACATTGGCGTTGTGCTGCTCATATTCATAAGGGCAACCAGTGATCCCACGCAGGCGCCGGTGCCGAAACGTACACTACCGACCATAGAATTTGCCGAGCCGGCGGCATTCGGGAATTTTTCTAAAATGGACGCCATGGCGTTGgaag                                                                          <  1:458119/219‑1 (MQ=255)                                                                         aTTATAAATTGCGCGCGGTTAAAAAATAATAGCAAACCACCGCCATCAACGAACACGCCGCCATGGTCAGCAACATTGGCGTTGTGCTGCTCATATTCATAAGGGCAACCAGTGATCCCACGCAGGCGCCGGTGCCGAAACGTACACTACCGACCATAGAATTTGCCGAGCCGGCGGCATTCGGGAATTTTTCTAAAATGGACGCCATGGCGTTGGAAGAAATCAGCGAGTTTTGTCCCACAAAAATCGCa                                          >  1:507999/1‑251 (MQ=255)                                                                         aTTATAAATTGCGCGCGGTTAAAAAATAATAGCAAACCACCGCCATCAACGAACACGCCGCCATGGTCAGCAACATTGGCGTTGTGCTGCTCATATTCATAAGGGCAACCAGTGATCCCACGCAGGCGCCGGTGCCGAAACGTACACTACCGACCATAGAATTTGCCGAGCCGGCGGCATTCGGGAATTTTTCTAAAATGGACGCCATGGCGTTGGAAGAAATCAGCGAGTTTTGTCCCACAAAAATCGCa                                          >  1:328278/1‑251 (MQ=255)                                                                                          gTTAAAAAATAATAGCAAACCACCGCCATCAACGAACACGCCGCCATGGTCAGCAACATTGGCGTTGTGCTGCTCATATTCATAAGGGCAACCAGTGATCCCACGCAGGCGCCGGTGCCGAAACGTACACTACCGACCATAGAATTTGCCGAGCCGGCGGCATTCGGGAATTTTTCTAAAATGGACGCCATGGCGTTGGAAGAAATCAGCGAGTTTTGTCCCACAAAAATCGCAATGCcaattgcaattg                          >  1:692873/1‑250 (MQ=255)                                                                                                 aataatAGCAAACCACCGCCATCAACGAACACGCCGCCATGGTCAGCAACATTGGCGTTGTGCTGCTCATATTCATAAGGGCAACCAGTGATCCCACGCAGGCGCCGGTGCCGAAACGTACACTACCGACCATAGAATTTGCCGAGCCGGCGGCATTCGGGAATTTTTCTAAAATGGACGCCATGGCGTTGGAAGAAATCAGCGAGTTTTGTCCCACAAAAATCGCAATGCCAATTGCAATTGccctgtag                  <  1:30640/251‑6 (MQ=255)                                                                                                      taGCAAACCACCGCCATCAACGAACACGCCGCCATGGTCAGCAACATTGGCGTTGTGCTGCTCATATTCATAAGGGCAACCAGTGATCCCACGCAGGCGCCGGTGCCGAAACGTACACTACCGACCATAGAATTTGCCGAGCCGGCGGCATTCGGGAATTTTTCTAAAATGGACGCCATGGCGTTGGAAGAAATCAGCGAGTTTTGTCCCACAAAAATCGCAATGCCAATTGCAATTGccctgtagcggag             >  1:205039/1‑241 (MQ=255)                                                                                                      taGCAAACCACCGCCATCAACGAACACGCCGCCATGGTCAGCAACATTGGCGTTGTGCTGCTCATATTCATAAGGGCAACCAGTGATCCCACGCAGGCGCCGGTGCCGAAACGTACACTACCGACCATAGAATTTGCCGAGCCGGCGGCATTCGGGAATTTTTCTAAAATGGACGCCATGGCGTTGGAAGAAATCAGCGAGTTTTGTCCCACAAAAATCGCAATGCCAATTGCAATTGccctgtagcggag             <  1:764158/251‑11 (MQ=255)                                                                                                      taGCAAACCACCGCCATCAACGAACACGCAGCCATGGTCAGCAACAGTGGCGTTGTGCTGCTCATATTCATAAGGGCAACCAGTGATCCCACGCAGGCGCCGGTGCCGAAAAGTACACTACCGACCATAGAATTTGCCGAGCCGGCGGCATTCGGGAATTTTTCTAAAATGGACGCCATGGCGTTGGAAGAAATCAGCGAGTTTTGTCCCACAAAAATCGCAATGACAATTGCAATTGccctgtagcggag             <  1:464602/251‑11 (MQ=255)                                                                                                                 cGCCATCAACGAACACGCCGCCATGGTCAGCAACATTGGCGTTGTGCTGCTCATATTCATAAGGGCAACCAGTGATCCCACGCAGGCGCCGGTGCCGAAACGTACACTACCGACCATAGAATTTGCCGAGCCGGCGGCATTCGGGAATTTTTCTAAAATGGACGCCATGACGTTGGAAGAAATCAGCGAGTTTTGTCCCACAAAAATCGCAATGCCAATTGCAATTGccctgtagcggagcgagcaatttg  >  1:446493/1‑230 (MQ=255)                                                                                                                        aaCGAACACGCCGCCATGGTCAGCAACATTGGCGTTGTGCTGCTCATATTCATAAGGGCAACCAGTGATCCCACGCAGGCGCCGGTGCCGAAACGTACACTACCGACCATAGAATTTGCCGAGCCGGCGGCATTCGGGAATTTTTCTAAAATGGACGCCATGGCGTTGGAAGAAATCAGCGAGTTTTGTCCCACAAAAATCGCAATGCCAATTGCAATTGccctgtag                  >  1:861/1‑223 (MQ=255)                                                                                                                                    gccATGGTCAGCAACATTGGCGTTGTGCTGCTCATATTCATAAGGGCAACCAGTGATCCCACGCAGGCGCCGGTGCCGAAACGTACACTACCGACCATAGAATTTGCCGAGCCGGCGGCATTCGGGAATTTTTCTAAAATGGACGCCATGGCGTTGGAAGAAAt                                                                      <  1:59172/164‑1 (MQ=255)                                                                                                                                    gccATGGTCAGCAACATTGGCGTTGTGCTGCTCATATTCATAAGGGCAACCAGTGATCCCACGCAGGCGCCGGTGCCGAAACGTACACTACCGACCATAGAATTTGCCGAGCCGGCGGCATTCGGGAATTTTTCTAAAATGGACGCCATGGCGTTGGAAGAAATCAGCGAGTTTTGTCCCACAAAAATCGCAATGCCAATTGCAATTGccctgtag                  >  1:315677/1‑211 (MQ=255)                                                                                                                                                 aCATTGGCGTTGTGCTGCTCATATTCATAAGGGCAACCAGTGATCCCACGCAGGCGCCGGTGCCGAAACGTACACTACCGACCATAGAATTTGCCGAGCCGGCGGCATTCGGGAATTTTTCTAAAATGGACGCCATGGCGTTGGAAGAAATCAGCGAGTTTTGTCCCACAAAAATCGCAATGCcaattgc                               >  1:509933/1‑190 (MQ=255)                                                                                                                                                                 gctCATATTCATAAGGGCAACCAGTGATCCCACGCAGGCGCCGGTGCCGAAACGTACACTACCGACCATAGAATTTGCCGAGCCGGCGGCATTCGGGAATTTTTCTAAAATGGACGCCATGGCGTTGGAAGAAATCAGCGAGTTTTGTCCCACAAAAATCGCAATGCcaattgc                               <  1:110978/174‑1 (MQ=255)                                                                                                                                                                     atatTCATAAGGGCAACCAGTGATCCCACGCAGGCGCCGGTGCCGAAACGTACACTACCGACCATAGAATTTGCCGAGCCGGCGGCATTCGGGAATTTTTCTAAAATGGACGCCATGGCGTTGGAAGAAATCAGCGAGTTTTGTCCCACAAAAATCGCAATGCcaattgc                               <  1:274086/170‑1 (MQ=255)                                                                                                                                                                                                                    aCGTACACTACCGACCATAGAATTTGCCGAGCCGGCGGCATTCGGGAATTTTTCTAAAATGGACGCCATGGCGTTGGAAGAAATCAGCGAGTTTTGTCCCACAAAAATCGCAATGCCAATTGCAATTGccctgtagcggag             <  1:279897/141‑11 (MQ=255)                                                                                                                                                                                                                      gTACACTACCGACCATAGAATTTGCCGAGCCGGCGGCATTCGGGAATTTTTCTAAAATGGACGCCATGGCGTTGGAAGAAATCAGCGAGTTTTGTCCCACAAAAATCGCAATGCCAATTGCAATTGccctgtag                  >  1:256123/1‑129 (MQ=255)                                                                                                                                                                                                                             aCCGACCATAGAATTTGCCGAGCCGGCGGCATTCGGGAATTTTTCTAAAATGGACGCCATGGCGTTGGAAGAAATCAGCGAGTTTTGTCCCACAAAAATCGCAATGCCAATTGCAATTGccctgtagcgg               <  1:382534/130‑9 (MQ=255)                                                                                                                                                                                                                                                    |                                                                                                                        GTACTTTGAGATATTTTTGATTTAGAACAAATTTATCTGTCCGTTGCAGATTACAATAATGCGCTTTTTAAAATTATAAATTGCGCGCGGTTAAAAAATAATAGCAAACCACCGCCATCAACGAACACGCCGCCATGGTCAGCAACATTGGCGTTGCGCTGCTCATATTCATAAGGGCAACCAGTG‑TCCCACGCAGGCGCCGGTGCCGAAACGTACACTACCGACCATAGAATTTGCCGAGCAGGCGGCATTCGGGAATTTTTCTAAAATGGACGCCATGGCGTTGGAAGAAATCAGCGAGTTTTGTCCCACAAAAATCGCAATGCCAATTGCAATTGCCCAAAAGCCCAAATTCAGCAACA  >  1/26532‑26893

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑