Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,587,212	A→G	intergenic (‑142584/‑54552)	unknown ← / → unknown	tRNA‑Met‑CAT/tRNA‑Gly‑GCC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,587,212	0	A	G	100.0%	177.7 / NA	66	intergenic (‑142584/‑54552)	unknown/unknown	tRNA‑Met‑CAT/tRNA‑Gly‑GCC
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (35/31);  total (35/31)

CCCAAAGGCGATAAGAATAAAGAGGTTAGCGCAAACAATGCTACTTCACAGCACAACGCCATGGCAATGGGAAAACCCAGCTTCAGCAATTTCCCCAGCGTGCGTCTATTCGGGCGTTCAATGATATTTGCGAAAACCTTCAAATCACGTTGATTTTTCGCCCGCATACAGTATGCCAACATCAACAGACACATCACCCAGTTTACAATTGCCGTGGCAATGCCACAGCCCACGGCGCCGAATGCCGACACACCCAATTTGCCATAAATAAAAATATAATTAAGAGGAATATTGATCAGCAAGCCGAGAAAGGTGATCACCATCGCTGGTTTAGTTTTCGCGATGCCGTCATTTAAACAACGAAAATTCACCATCAACAAATAGCCCGGCAAGCCCCAAACCATGGCGTGCAAATAACCGATAGTAATGTCCGCCAAGCGTTGTTCCATGTCCATTTTTTGTAGTACGAAATCGCTGCTATAAATC  >  1/1586965‑1587450                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                               cccAAAGGCGATAAGAATAAAGAGGTTAGCGCAAACAATGCTACTTCACAGCACAACGCCATAGCAATGGGGAAACCCAGCTTCAGCAATTTCCCCAGCGTGCGTCTATTCGGGCGTTCAATGATATTTGCGAAAACCTTCAAATCACGTTGATTTTTCGCCCGCATACAGTATGCCAACATCAACAGACACATCACCCAGTTTACAATTGCCGTGGCAATGCCACAGCCCACGGCGCCGAATGCCGgcac                                                                                                                                                                                                                                             <  1:378727/251‑1 (MQ=255)     aaGGCGATAAGAATAAAGAGGTTAGCGCAAACAATGCTACTTCACAGCACAACGCCATAGCAATGGGGAAACCCAGCTTCAGCAATTTCCCCAGCGTGCGTCTATTCGGGCGTTCAATGATATTTGCGAAAACCTTCAAATCACGTTGATTTTTCGCCCGCATACAGTATGCCAACATCAACAGACACATCACCCAGTTTACAATTGCCGTGGCAATGCCACAGCCCACGGCGCCGAATGCCGGCACAccc                                                                                                                                                                                                                                         <  1:638441/251‑1 (MQ=255)         cGATAAGAATAAAGAGGTTAGCGCAAACAATGCTACTTCACAGCACAACGCCATAGCAATGGGGAAACCCAGCTTCAGCAATTTCCCCAGCGTGCGTCTATTCGGGCGTTCAATGATATTTGCGAAAACCTTCAAATCACGTTGATTTTTCGCCCGCATACAGTATGCCAACATCAACAGACACATCACCCAGTTTACAATTGCCGTGGCAATGCCACAGCCCACGGCGCCGAATGCCGGCACACCCAAtt                                                                                                                                                                                                                                     <  1:848353/251‑1 (MQ=255)            tAAGAATAAAGAGGTTAGCGCAAACAATGCTACTTCACAGCACAACGCCATAGCAATGGGGAAACCCAGCTTCAGCAATTTCCCCAGCGTGCGTCTATTCGGGCGTTCAATGATATTTGCGAAAACCTTCAAATCACGTTGATTTTTCGCCCGCATACAGTATGCCAACATCAACAGACACATCACCCAGTTTACAATTGCCGTGGCAATGCCACAGCCCACGGCGCCGAATGCCGGCACACCCAATTTGc                                                                                                                                                                                                                                  <  1:893695/251‑1 (MQ=255)               gAATAAAGAGGTTAGCGCAAACAATGCTACTTCACAGCACAACGCCATAGCAATGGGGAAACCCAGCTTCAGCAATTTCCCCAGCGTGCGTCTATTCGGGCGTTCAATGATATTTGCGAAAACCTTCAAATCACGTTGATTTTTCGCCCGCATACAGTATGCCAACATCAACAGACACATCACCCAGTTTACAATTGCCGTGGCAATGCCACAGCCCACGGCGCCGAATGCCGGCACACCCAATTTGccgt                                                                                                                                                                                                                               >  1:746887/1‑249 (MQ=255)               gAATAAAGAGGTTAGCGCAAACAATGCTACTTCACAGCACAACGCCATAGCAATGGGGAAACCCAGCTTCAGCAATTTCCCCAGCGTGCGTCTATTCGGGCGTTCAATGATATTTGCGAAAACCTTCAAAT‑ACGTTGATTTTTCGCCCGCATACAGTATGCCAACATCAACAGACACATCACCCAGTTTACAATTGCCGTGGCAATGCCACAGCCCACGGCGCCGAATGCCGGCACACCCAATTTGc                                                                                                                                                                                                                                  >  1:409825/1‑247 (MQ=255)                aaTAAAGAGGTTAGCGCAAACAATGCTACTTCACAGCACAACGCCATAGCAATGGGGAAACCCAGCTTCAGCAATTTCCCCAGCGTGCGTCTATTCGGGCGTTCAATGATATTTGCGAAAACCTTCAAATCACGTTGATTTTTCGCCCGCATACAGTATGCCAACATCAACAGACACATCACCCAGTTTACAATTGCCGTGGCAATGCCACAGCCCACGGCGCCGAATGCCGGCACACCCAATTTGccg                                                                                                                                                                                                                                <  1:860582/249‑2 (MQ=255)                  tAAAGAGGTTAGCGCAAACAATGCTACTTCACAGCACAACGCCATAGCAATGGGGAAACCCAGCTTCAGCAATTTCCCCAGCGTGCGTCTATTCGGGCGTTCAATGATATTTGCGAAAACCTTCAAATCACGTTGATTTTTCGCCCGCATACAGTATGCCAACATCAACAGACACATCACCCAGTTTACAATTGCCGTGGCAATGCCACAGCCCACGGCGCCGAATGCCGGCACACCCAATTTGCCgtaaa                                                                                                                                                                                                                            >  1:326364/1‑251 (MQ=255)                   aaaGAGGTTAGCGCAAACAATGCTACTTCACAGCACAACGCCATAGCAATGGGGAAACCCAGCTTCAGCAATTTCCCCAGCGTGCGTCTATTCGGGCGTTCAATGATATTTGCGAAAACCTTCAAATCACGTTGATTTTTCGCCCGCATACAGTATGCCAACATCAACAGACACATCACCCAGTTTACAATTGCCGTGGCAATGCCACAGCCCACGGCGCCGAATGCCGGCACACCCAAttt                                                                                                                                                                                                                                    >  1:370043/1‑242 (MQ=255)                                            cTTCACAGCACAACGCCATAGCAATGGGGAAACCCAGCTTCAGCAATTTCCCCAGCGTGCGTCTATTCGGGCGTTCAATGATATTTGCGAAAACCTTCAAATCACGTTGATTTTTCGCCCGCATACAGGATGCCAACATCAACAGAAACATCACCCAGTTTACAATTGCCGTGGCAATGCCACAGCCCACGGCGCCGAAAGCCGGCAAACCACATTTGCCGGAAATAAAAATATAATTAag                                                                                                                                                                                                            >  1:238474/1‑241 (MQ=255)                                             ttCACAGCACAACGCCATAGCAATGGGGAAACCCAGCTTCAGCAATTTCCCCAGCGTGCGTCTATTCGGGCGTTCAATGATATTTGCGAAAACCTTCAAATCACGTTGATTTTTCGCCCGCATACAGTATGCCAACATCAACAGACACATCACCCAGTTTACAATTGCCGTGGCAATGCCACAGCCCACGGCGCCGAATGCCGGCACACCCAATTTGCCGTAAATAAAAATATAATTAAGAGGAATATTGa                                                                                                                                                                                                 <  1:74295/251‑1 (MQ=255)                                               cacaGCACAACGCCATAGCAATGGGGAAACCCAGCTTCAGCAATTTCCCCAGCGTGCGTCTATTCGGGCGTTCAATGATATCTGCGAAAACCTTCAAATCACGTTGATTTTTCGCCCGCATACAGTATGCCAACATCAACAGACACATCACCCAGTTTACAATTGCCGTGGCAATGCCACAGCCCACGGCGCCGAATGCCGGCACACCCAATTTGCCGTAAATAAAAATATAATTAAGAGGAATATTGATc                                                                                                                                                                                               <  1:423713/251‑1 (MQ=255)                                                   gCACAACGCCATAGCAATGGGGAAACCCAGCTTCAGCAATTTCCCCAGCGTGCGTCTATTCGGGCGTTCAATGATATTTGCGAAAACCTTCAAATCACGTTGATTTTTCGCCCGCATACAGTATGCCAACATCAACAGACACATCACCCAGTTTACAATTGCCGTGGCAATGCCACAGCCCACGGCGCCGAATGCCGGCACACCCAATTTGCCGTAAATAAAAATATAATTAAGAgg                                                                                                                                                                                                         >  1:200338/1‑237 (MQ=255)                                                      caACGCCATAGCAATGGGGAAACCCAGCTTCAGCAATTTCCCCAGCGTGCGTCTATTCGGGCGTTCAATGATATTTGCGAAAACCTTCAAATCACGTTGATTTTTCGCCCGCATACAGTATGCCAACATCAACAGACACATCACCCAGTTTACAATTGCCGTGGCAATGCCACAGCCCACGGCGCCGAATGCCGGCACACCCAATTTGCCGTAAATAAAAATATAATTAAGAGGAATATTGATCAGCAAGc                                                                                                                                                                                        <  1:371/251‑1 (MQ=255)                                                         cGCCATAGCAATGGGGAAACCCAGCTTCAGCAATTTCCCCAGCGTGCGTCTATTCGGGCGTTCAATGATATTTGCGAAAACCTTCAAATCACGTTGATTTTTCGCCCGCATACAGTATGCCAACATCAACAGACACATCACCCAGTTTACAATTGCCGTGGCAATGCCACAGCCCACGGCGCCGAATGCCGGCACACCCAATTTGCCGTAAATAAAAATATAATTAAGAgg                                                                                                                                                                                                         <  1:266794/231‑1 (MQ=255)                                                           ccATAGCAATGGGGAAACCCAGCTTCAGCAATTTCCCCAGCGTGCGTCTATTCGGGCGTTCAATGATATTTGCGAAAACCTTCAAATCACGTTGATTTTTCGCCCGCATACAGTATGCCAACATCAACAGACACATCACCCAGTTTACAATTGCCGTGGCAATGCCACAGCCCACGGCGCCGAATGCCGgcac                                                                                                                                                                                                                                             >  1:98041/1‑193 (MQ=255)                                                               agCAATGGGGAAACCCAGCTTCAGCAATTTCCCCAGCGTGCGTCTATTCGGGCGTTCAATGATATTTGCGAAAACCTTCAAATCACGTTGATTTTTCGCCCGCATACAGTATGCCAACATCAACAGACACATCACCCAGTTTACAATTGCCGTGGCAATGCCACAGCCCACGGCGCCGAATGCCGGCACACCCAATTTGCCGTAAATAAAAATATAATTa                                                                                                                                                                                                              >  1:355430/2‑220 (MQ=255)                                                                gCAATGGGGAAACCCAGCTTCAGCAATTTCCCCAGCGTGCGTCTATTCGGGCGTTCAATGATATTTGCGAAAACCTTCAAATCACGTTGATTTTTCGCCCGCATACAGTATGCCAACATCAACAGACACATCACCCAGTTTACAATTGCCGTGGCAATGCCACAGCCCACGGCGCCGAATGCCGGCACACCCAATTTGCCGTAAATAAAAATATAATTAAGAGGAATATTGATCAGCaa                                                                                                                                                                                          <  1:320600/239‑1 (MQ=255)                                                                gCAATGGGGAAACCCAGCTTCAGCAATTTCCCCAGCGTGCGTCTATTCGGGCGTTCAATGATATTTGCGAAAACCTTCAAATCACGTTGATTTTTCGCCCGCATACAGTATGCCAACATCAACAGACACATCACCCAGTTTACAATTGCCGTGGCAATGCCACAGCCCACGGCGCCGAATGCCGGCACACCCAATTTGCCGTAAATAAAAATATAATTAAGAGGAATATTGATCAGCAAGCCGAGAAAGGt                                                                                                                                                                              >  1:461352/1‑251 (MQ=255)                                                                 cAATGGGGAAACCCAGCTTCAGCAATTTCCCCAGCGTGCGTCTATTCGGGCGTTCAATGATATTTGCGAAAACCTTCAAATCACGTTGATTTTTCGCCCGCATACAGTATGCCAACATCAACAGACACATCACCCAGTTTACAATTGCCGTGGCAATGCCACAGCCCACGGCGCCGAATGCCGGCACACCCAATTTGCCGTAAATAAAAATATAATTAAGAGGAATATTGATCAGCAAGCCGAGAAAgg                                                                                                                                                                               <  1:163974/249‑1 (MQ=255)                                                                    tGGGGAAACCCAGCTTCAGCAATTTCCCCAGCGTGCGTCTATTCGGGCGTTCAATGATATTTGCGAAAACCTTCAAATCACGTTGATTTTTCGCCCGCATACAGTATGCCAACATCAACAGACACATCACCCAGTTTACAATTGCCGTGGCAATGCCACAGCCCACGGCGCCGAATGCCGGCACACCCAATTTGCCGTAAATAAAAATATAATTAAGAGGAATATTGATCAGCAAGCCGAGAAAGGTGATc                                                                                                                                                                          <  1:291954/251‑1 (MQ=255)                                                                                       cAATTTCCCCAGCGTGCGTCTATTCGGGCGTTCAATGATATTTGCGAAAACCTTCAAATCACGTTGATTTTTCGCCCGCATACAGTATGCCAACATCAACAGACACATCACCCAGTTTACAATTGCCGTGGCAATGCCACAGCCCACGGCGCCGAATGCCGGCACACCCAATTTGCCGTAAATAAAAATATAATTAAGAGGAATATTGATCAGCAAGCCGAGAAAGGTGATCACCATCGCTGGTTTAGtt                                                                                                                                                        <  1:395344/250‑1 (MQ=255)                                                                                                  gcgtgcgtCTATTCGGGCGTTCAATGATATTTGCGAAAACCTTCAAATCACGTTGATTTTTCGCCCGCATACAGTATGCCAACATCAACAGACACATCACCCAGTTTACAATTGCCGTGGCAATGCCACAGCCCACGGCGCCGAATGCCGGCACACCCAATTTGCCGTAAATAAAAATATAATTAAGAGGAATATTGATCAGCAAGCCGAGAAAGGTGATCACCATCGCTGGTTTAGTTTTCGCGATGCCg                                                                                                                                            >  1:645691/1‑251 (MQ=255)                                                                                                   cgtgcgtCTATTCGGGCGTTCAATGATATTTGCGAAAACCTTCAAATCACGTTGATTTTTCGCCCGCATACAGTATGCCAACATCAACAGACACATCACCCAGTTTACAATTGCCGTGGCAATGCCACAGCCCACGGCGCCGAATGCCGGCACACCCAATTTGCCGTAAATAAAAATATAATTAAGAGGAATATTGATCAGCAAGCCGAGAAAGGTGATCACCATCGCTGGTTTAGTTTTCGCGATGc                                                                                                                                              <  1:113577/248‑1 (MQ=255)                                                                                                   cgtgcgtCTATTCGGGCGTTCAATGATATTTGCGAAAACCTTCAAATCACGTTGATTTTTCGCCCGCATACAGTATGCCAACATCAACAGACACATCACCCAGTTTACAATTGCCGTGGCAATGCCACAGCCCACGGCGCCGAATGCCGGCACACCCAATTTGCCGTAAATAAAAATATAATTAAGAGGAATATTGATCAGCAAGCCGAGAAAGGTGATCACCATCGCTGGTTTAGTTTTCGCGATGCCGt                                                                                                                                           <  1:391110/251‑1 (MQ=255)                                                                                                     tgcgtCTATTCGGGCGTTCAATGATATTTGCGAAAACCTTCAAATCACGTTGATTTTTCGCCCGCATACAGTATGCCAACATCAACAGACACATCACCCAGTTTACAATTGCCGTGGCAATGCCACAGCCCACGGCGCCGAATGCCGGCACACCCAATTTGCCGTAAATAAAAATATAATTAAGAGGAatat                                                                                                                                                                                                    >  1:608080/1‑192 (MQ=255)                                                                                                          cTATTCGGGCGTTCAATGATATTTGCGAAAACCTTCAAATCACGTTGATTTTTCGCCCGCATACAGTATGCCAACATCAACAGACACATCACCCAGTTTACAATTGCCGTGGCAATGCCACAGCCCACGGCGCCGAATGCCGGCACACCCAATTTGCCGTAAATAAAAATATAATTAAGAGGAatat                                                                                                                                                                                                    >  1:688606/1‑187 (MQ=255)                                                                                                            aTTCGGGCGTTCAATGATATTTGCGAAAACCTTCAAATCACGTTGATTTTTCGCCCGCATACAGTATGCCAACATCAACAGACACATCACCCAGTTTACAATTGCCGTGGCAATGCCACAGCCCACGGCGCCGAATGCCGGCACACCCAATTTGCCGTAAATAAAAATATAATTAAGAGGAATATTGATCAGCAAGCCGAGAAAGGTGATCACCATCGCTGGTTTAGTTTTCGCGATGCCGTCATTTAaac                                                                                                                                  <  1:47134/251‑1 (MQ=255)                                                                                                                     ttCAATGATATTTGCGAAAACCTTCAAATCACGTTGATTTTTCGCCCGCATACAGTATGCCAACATCAACAGACACATCACCCAGTTTACAATTGCCGTGGCAATGCCACAGCCCACGGCGCCGAATGCCGGCACACCCAATTTGCCGTAAATAAAAATATAATTAAGAGGAATATTGATCAGCAAGCCGAGAAAGGTGATCACCATCGCTGGTTTAGTTTTCGCGATGCCGTCATTTAAACAACGAAAAt                                                                                                                         >  1:685837/1‑251 (MQ=255)                                                                                                                     ttCAATGATATTTGCGAAAACCTTCAAATCACGTTGATTTTTCGCCCGCATACAGTATGCCAACATCAACAGACACATCACCCAGTTTACAATTGCCGTGGCAATGCCACAGCCCACGGCGCCGAATGCCGGCACACCCAATTTGCCGTAAATAAAAATATAATTAAGAGGAATATTGATCAGCAAGCCGAGAAAGGTGATCACCATCGCTGGTTTAGTTTTCGCGATGCCGTCATTTAAACAACGAAAAt                                                                                                                         >  1:375180/1‑251 (MQ=255)                                                                                                                       cAATGATATTTGCGAAAACCTTCAAATCACGTTGATTTTTCGCCCGCATACAGTATGCCAACATCAACAGACACATCACCCAGTTTACAATTGCCGTGGCAATGCCACAGCCCACGGCGCCGAATGCCGGCACACCCAATTTGCCGTAAATAAAAATATAATTAAGAGGAatat                                                                                                                                                                                                    >  1:598748/1‑174 (MQ=255)                                                                                                                       cAATGATATTTGCGAAAACCTTCAAATCACGTTGATTTTTCGCCCGCATACAGTATGCCAACATCAACAGACACATCACCCAGTTTACAATTGCCGTGGCAATGCCACAGCCCACGGCGCCGAATGCCGGCACACCCAATTTGCCGTAAATAAAAATATAATTAAGAGGAATATTGATc                                                                                                                                                                                               <  1:190999/179‑1 (MQ=255)                                                                                                                                         ccTTCAAATCACGTTGATTTTTCGCCCGCATACAGTATGCCAACATCAACAGACACATCACCCAGTTTACAATTGCCGTGGCAATGCCACAGCCCACGGCGCCGAATGCCGGCACACCCAATTTGCCGTAAATAAAAATATAATTAAGAGGAatat                                                                                                                                                                                                    <  1:635929/156‑1 (MQ=255)                                                                                                                                          cTTCAAATCACGTTGATTTTTCGCCCGCATACAGTATGCCAACATCAACAGACACATCACCCAGTTTACAATTGCCGTGGCAATGCCACAGCCCACGGCGCCGAATGCCGGCACACCCAATTTGCCGTAAATAAAAATATAATTAAGAGGAATATTGATCAGCAAGCCGAGAAAGGTGATCACCATCGCTGGTTTAGTTTTCGCGATGCCGTCATTTAAACAACGAAAATTCACCATCAACAAATAGCCCg                                                                                                    >  1:154280/1‑251 (MQ=255)                                                                                                                                                       tGATTTTTCGCCCGCATACAGTATGCCAACATCAACAGACACATCACCCAGTTTACAATTGCCGTGGCAATGCCACAGCCCACGGCGCCGAATGCCGGCACACCCAATTTGCCGTAAATAAAAATATAATTAAGAGGAATATTGATCAGCAAGCCGAGAAAGGTGATCACCATCGCTGGTTTAGTTTTCGCGATGCCGTCATTTAAACAACGAAAATTCACCATCAACAAATAGCCCGGCAAGCCCCAAAc                                                                                       <  1:99861/251‑1 (MQ=255)                                                                                                                                                          tttttCGCCCGCATACAGTATGCCAACATCAACAGACACATCACCCAGTTTACAATTGCCGTGGCAATGCCACAGCCCACGGCGCCGAATGCCGGCACACCCAATTTGCCGTAAATAAAAATATAATTAAGAGGAATATTGATCAGCAAGCCGAGAAAGGTGATCACCATCGCTGGTTTAGTTTTCGCGATGCCGTCATTTAAACAACGAAAATTCACCATCAACAAATAGCCCGGCAAGCCCCAAAc                                                                                       >  1:666131/1‑248 (MQ=255)                                                                                                                                                                 cccGCATACAGTATGCCAACATCAACAGACACATCACCCAGTTTACAATTGCCGTGGCAATGCCACAGCCCACGGCGCCGAATGCCGGCACACCCAATTTGCCGTAAATAAAAATATAATTAAGAGGAATATTGATCAGCAAGCCGAGAAAGGTGATCACCATCGCTGGTTTAGTTTTCGCGATGCCGTCATTTAAACAACGAAAATTCACCATCAACAAATAGcc                                                                                                      <  1:370089/226‑1 (MQ=255)                                                                                                                                                                   cGCATACAGTATGCCAACATCAACAGACACATCACCCAGTTTACAATTGCCGTGGCAATGCCACAGCCCACGGCGCCGAATGCCGGCACAcc                                                                                                                                                                                                                                          <  1:323215/92‑1 (MQ=255)                                                                                                                                                                   cGCATACAGTATGCCAACATCAACAGACACATCACCCAGTTTACAATTGCCGTGGCAATGCCACAGCCCACGGCGCCGAATGCCGGCACACCCAATTTGCCGTAAATAAAAATATAATTAAGAGGAATATTGATCAGCAAGCCGAGAAAGGTGATc                                                                                                                                                                          >  1:889892/1‑156 (MQ=255)                                                                                                                                                                   cGCATACAGTATGCCAACATCAACAGACACATCACCCAGTTTACAATTGCCGTGGCAATGCCACAGCCCACGGCGCCGAATGCCGGCACACCCAATTTGCCGTAAATAAAAATATAATTAAGAGGAATATTGATCAGCAAGCCGAGAAAGGTGATCACCATCGCTGGTTTAGTTTTCGCGATGCCGTCATTTAAACAACGAAAATTCACCATCAACAAATAGCCCGGCAAGCCCCAAAcc                                                                                      >  1:196464/1‑240 (MQ=255)                                                                                                                                                                   cGCATACAGTATGCCAACATCAACAGACACATCACCCAGTTTACAATTGCCGTGGCAATGCCACAGCCCACGGCGCCGAATGCCGGCACACCCAATTTGCCGTAAATAAAAATATAATTAAGAGGAATATTGATCAGCAAGCCGAGAAAGGTGATCACCATCGCTGGTTTAGTTTTCGCGATGCCGTCATTTAAACAACGAAAATTCACCATCAACAAATAGCCCGGCAAGCCCCAAACCATGGCGTGCaa                                                                           >  1:804952/1‑251 (MQ=255)                                                                                                                                                                   cGCATACAGTATGCCAACATCAACAGACACATCACCCAGTTTACAATTGCCGTGGCAATGCCACAGCCCACGGCGCCGAATGCCGGCACACCCAATTTGCCGTAAATAAAAATATAATTAAGAGGAATATTGATCAGCAAGCCGAGAAAGGTGATCACCATCGCTGGTTTAGTTTTCGCGATGCCGTCATTTAAACAACGAAAATTCACCATCAACAAATAGCCCGGCAAGCCCCAAACCATGGCGTGCaa                                                                           >  1:670065/1‑251 (MQ=255)                                                                                                                                                                     cATACAGTATGCCAACATCAACAGACACATCACCCAGTTTACAATTGCCGTGGCAATGCCACAGCCCACGGCGCCGAATGCCGGCACACCCAATTTGCCGTAAATAAAAATATAATTAAGAGGAATATTGATCAGCAAGCCGAGAAAGGTGGTCACCATCGCTGGTTTAGTTTTCGCGATGCCGTCATTTAAACAACGAAAATTCAc                                                                                                                     <  1:461630/207‑1 (MQ=255)                                                                                                                                                                         cAGTATGCCAACATCAACAGACACATCACCCAGTTTACAATTGCCGTGGCAATGCCACAGCCCACGGCGCCGAATGCCGGCACACCCAATTTGCCGTAAATAAAAATATAATTAAGAGGAATATTGATCAGCAAGCCGAGAAAGGTGATCACCATCGCTGGTTTAGTTTTCGCGATGCCGTCATTTAAACAACGAAAATTCACCATCAACAAATAGCCCGGCAAGCCCCAAACCATGGCGTGCAAATAAc                                                                      >  1:12072/1‑250 (MQ=255)                                                                                                                                                                         cAGTATGCCAACATCAACAGACACATCACCCAGTTTACAATTGCCGTGGCAATGCCACAGCCCACGGCGCCGAATGCCGGCACACCCAATTTGCCGTAAATAAAAATATAATTAAGAGGAATATTGATCAGCAAGCCGAGAAAGGTGATCACCATCGCTGGTTTAGTTTTCGCGATGCCGTCATTTAAACAACGAAAATTCACCATCAACAAATAGCCCGGCAAGCCCCAAACCATGGCGTGCAAATAAc                                                                      >  1:53651/1‑250 (MQ=255)                                                                                                                                                                         cAGTATGCCAACATCAACAGACACATCACCCAGTTTACAATTGCCGTGGCAATGCCACAGCCCACGGCGCCGAATGCCGGCACACCCAATTTGCCGTAAATAAAAATATAATTAAGAGGAATATTGATCAGCAAGCCGAGAAAGGTGATCACCATCGCTGGTTTAGTTTTCGCGATGCCGTCATTTAAACAACGAAAATTCACCATCAACAAATAGCCCGGCAAGCCCCAAACCATGGCGTGCAAATAAc                                                                      >  1:477162/1‑250 (MQ=255)                                                                                                                                                                           gTATGCCAACATCAACAGACACATCACCCAGTTTACAATTGCCGTGGCAATGCCACAGCCCACGGCGCCGAATGCCGGCACACCCAATTTGCCGTAAATAAAAATATAATTAAGAGGAATATTGATCAGCAAGCCGAGAAAGGTGATCACCATCGCTGGTTTAGTTTTCGCGATGCCGTCATTTAAACAACGAAAATTCACCATCAACAAATAGCCCGGCAAGCCCCAAAc                                                                                       >  1:517257/1‑231 (MQ=255)                                                                                                                                                                           gTATGCCAACATCAACAGACACATCACCCAGTTTACAATTGCCGTGGCAATGCCACAGCCCACGGCGCCGAATGCCGGCACACCCAATTTGCCGTAAATAAAAATATAATTAAGAGGAATATTGATCAGCAAGCCGAGAAAGGTGATCACCATCGCTGGTTTAGTTTTCGCGATGCCGTCATTTAAACAACGAAAATTCACCATCAACAAATAGCCCGGCAAGCCCCAAACCATGGCGTGCAAATAACCGa                                                                   >  1:224561/1‑251 (MQ=255)                                                                                                                                                                             aTGCCAACATCAACAGACACATCACCCAGTTTACAATTGCCGTGGCAATGCCACAGCCCACGGCGCCGAATGCCGGCACACCCAATTTGCCGTAAATAAAAATATAATTAAGAGGAATATTGATCAGCAAGCCGAGAAAGGTGATCACCATCGCTGGTTTAGTTTTCGCGATGCCGTCATTTAAACAACGAAAATTCACCATCAACAAATAGCCCGGCAAGCCCCAAACCATGGCGTGCAAATAACCGATa                                                                 >  1:508824/1‑251 (MQ=255)                                                                                                                                                                                 cAACATCAACAGACACATCACCCAGTTTACAATTGCCGTGGCAATGCCACAGCCCACGGCGCCGAATGCCGGCACACCCAATTTGCCGTAAATAAAAATATAATTAAGAGGAATATTGATCAGCAAGCCGAGAAAGGTGATCACCATCGCTGGTTTAGTTTTCGCGATGCCGTCATTTAAACAACGAAAATTCACCATCAACAAATAGCCCGGCAAGCCCCAAACCATGGCGTGCAAATAACCGATAGTaa                                                             <  1:567513/251‑1 (MQ=255)                                                                                                                                                                                  aaCATCAACAGACACATCACCCAGTTTACAATTGCCGTGGCAATGCCACAGCCCACGGCGCCGAATGCCGGCACACCCAATTTGCCGTAAATAAAAATATAATTAAGAGGAATATTGATCAGCAAGCCGAGAAAGGTGATCACCATCGCTGGTTTAGTTTTCGCGATGCCGTCATTTAAACAACGAAAATTCACCATCAACAAATAGCCCGGCAAGCCCCAAACCATGGCGTGCAAATAACCGATAGTAAt                                                            >  1:262259/1‑251 (MQ=255)                                                                                                                                                                                         aCAGACACATCACCCAGTTTACAATTGCCGTGGCAATGCCACAGCCCACGGCGCCGAATGCCGGCACACCCAATTTGCCGTAAATAAAAATATAATTAAGAGGAATATTGATCAGCAAGCCGAGAAAGGTGATCACCATCGCTGGTTTAGTTTTCGCGATGCCGTCATTTAAACAACGAAAATTCACCATCAACAAATAGCCCGGCAAGCCCCAAACCATGGCGTGCAAATAACCGATAGTAATg                                                           >  1:644/1‑245 (MQ=255)                                                                                                                                                                                         aCAGACACATCACCCAGTTTACAATTGCCGTGGCAATGCCACAGCCCACGGCGCCGAATGCCGGCACACCCAATTTGCCGTAAATAAAAATATAATTAAGAGGAATATTGATCAGCAAGCCGAGAAAGGTGATCACCATCGCTGGTTTAGTTTTCGCGATGCCGTCATTTAAACAACGAAAATTCACCATCAACAAATAGCCCGGCAAGCCCCAAACCATGGCGTGCAAATAACCGATAGTAATGTCCGc                                                      >  1:609479/1‑250 (MQ=255)                                                                                                                                                                                              cacaTCACCCAGTTTACAATTGCCGTGGCAATGCCACAGCCCACGGCGCCGAATGCCGGCACACCCAATTTGCCGTAAATAAAAATATAATTAAGAGGAATATTGATCAGCAAGCCGAGAAAGGTGATCACCATCGCTGGTTTAGTTTTCGCGATGCCGTCATTTAAACAACGAAAATTCACCATCAACAAATAGCCCGGCAAGCCCCAAACCATGGCGTGCAAATAACCGATAGTAATGTCCGCCAAGCg                                                >  1:700118/1‑251 (MQ=255)                                                                                                                                                                                               acaTCACCCAGTTTACAATTGCCGTGGCAATGCCACAGCCCACGGCGCCGAATGCCGGCACACCCAATTTGCCGTAAATAAAAATATAATTAAGAGGAATATTGATCAGCAAGCCGAGAAAGGTGATCACCATCGCTGGTTTAGTTTTCGCGATGCCGTCATTTAAACAACGAAAATTCACCATCAACAAATAGCCCGGCAAGCCCCAAACCATGGCGTGCAAATAACCGATAGTAATGTCCGCCAAGCgt                                               <  1:436082/251‑1 (MQ=255)                                                                                                                                                                                                         gTTTACAATTGCCGTGGCAATGCCACAGCCCACGGCGCCGAATGCCGGCACACCCAATTTGCCGTAAATaaaaa                                                                                                                                                                                                                      <  1:151374/74‑1 (MQ=255)                                                                                                                                                                                                         gTTTACAATTGCCGTGGCAATGCCACAGCCCACGGCGCCGAATGCCGGCACACCCAATTTGCCGTAAATAAAAATATAATTAAGAGGAATATTGATCAGCAAGCCGAGAAAGGTGATCACCATCg                                                                                                                                                                   <  1:223480/125‑1 (MQ=255)                                                                                                                                                                                                         gTTTACAATTGCCGTGGCAATGCCACAGCCCACGGCGCCGAATGCCGGCACACCCAATTTGCCGTAAATAAAAATATAATTAAGAGGAATATTGATCAGCAAGCCGAGAAAGGTGATCACCATCGCTGGTTTAGTTTTCGCGATGCCGTCATTTAaac                                                                                                                                  >  1:814243/1‑158 (MQ=255)                                                                                                                                                                                                         gTTTACAATTGCCGTGGCAATGCCACAGCCCACGGCGCCGAATGCCGGCACACCCAATTTGCCGTAAATAAAAATATAATTAAGAGGAATATTGATCAGCAAGCCGAGAAAGGTGATCACCATCGCTGGTTTAGTTTTCGCGATGCCGTCATTTAAACAACGAAAATTCACCATCAACAAATAGCCCGGCAAGCCCCAAACCATGGCGTGCAAATAACCGATAGTAATGTCCGCCAAGCGTTGTTCCATGt                                     >  1:752264/1‑251 (MQ=255)                                                                                                                                                                                                                  tGCCGTGGCAATGCCACAGCCCACGGCGCCGAATGCCGGCACACCCAATTTGCCGTAAATAAAAATATAATTAAGAGGAATATTGATCAGCAAGCCGAGAAAGGTGATCACCATCGCTGGTTTAGTTTTCGCGATGCCg                                                                                                                                            >  1:727227/1‑139 (MQ=255)                                                                                                                                                                                                                             tGCCACAGCCCACGGCGCCGAATGCCGGCACACCCAATTTGCCGTAAATAAAAATATAATTAAGAGGAATATTGATCAGCAAGCCGAGAAAGGTGATCACCATCGCTGGTTTAGTTTTCGCGATGCCGTCATTTAAACAACGAAAATTCACCATCAACAAATAGCCCGGCAAGCCCCAAACCATGGCGTGCAAATAACCGATAGTAATGTCCGCCAAGCGTTGTTCCATGTCCATTTTTTGTAGTACGaaa                 >  1:719904/1‑251 (MQ=255)                                                                                                                                                                                                                               ccACAGCCCACGGCGCCGAATGCCGGCACACCCAATTTGCCGTAAATAAAAATATAATTAAGAGGAATATTGATCAGCAAGCCGAGAAAGGTGATCACCATCGCTGGTTTAGTTTTCGCGATGCCGTCATTTAAACAACGAAAATTCACCATCAACAAATAGCCCGGCAAGCCCCAAACCATGGCGTGCAAATAACCGATAGTAATGTCCGCCAAGCGTTGTTCCATGTCCATTTTTTGTAGTACGAAAt                <  1:782654/250‑1 (MQ=255)                                                                                                                                                                                                                               ccACAGCCCACGGCGCCGAATGCCGGCACACCCAATTTGCCGTAAATAAAAATATAATTAAGAGGAATATTGATCAGCAAGCCGAGAAAGGTGATCACCATCGCTGGTTTAGTTTTCGCGATGCCGTCATTTAAACAACGAAAATTCACCATCAACAAATAGCCCGGCAAGCCCCAAACCATGGCGTGCAAATAACCGATAGTAATGTCCGCCAAGCGTTGTTCCATGTCCATTTTTTGTAGTACGAAAt                <  1:295560/250‑1 (MQ=255)                                                                                                                                                                                                                                   aGCCCACGGCGCCGAATGCCGGCACACCCAATTTGCCGTAAATAAAAATATAATTAAGAGGAATATTGATCAGCAAGCCGAGAAAGGTGATCACCATCGCTGGTTTAGTTTTCGCGATGCCGTCATTTAAACAACGAAAATTCACCATCAACAAATAGCCCGGCAAGCCCCAAACCATGGCGTGCAAATAACCGATAGTAATGTCCGCCAAGCGTTGTTCCATGt                                     >  1:49766/1‑225 (MQ=255)                                                                                                                                                                                                                                            cgcCGAATGCCGGCACACCCAATTTGCCGTAAATAAAAATATAATTAAGAGGAATATTGATCAGCAAGCCGAGAAAGGTGATCACCATCGCTGGTTTAGTTTTCGCGATGCCGTCATTTAAACAACGAAAATTCACCATCAACAAATAGCCCGGCAAGCCCCAAACCATGGCGTGCAAATAACCGATAGTAATGTCCGCCAAGCGTTGTTCCATGTCCATTTTTTGTAGTACGAAATCGCTGCTATAAAt   <  1:439368/250‑1 (MQ=255)                                                                                                                                                                                                                                            cgcCGAATGCCGGCACACCCAATTTGCCGTAAATAAAAATATAATTAAGAGGAATATTGATCAGCAAGCCGAGAAAGGTGATCACCATCGCTGGTTTAGTTTTCGCGATGCCGTCATTTAAACAACGAAAATTCACCATCAACAAATAGCCCGGCAAGCCCCAAACCATGGCGTGCAAATAACCGATAGTAATGTCCGCCAAGCGTTGTTCCATGTCCATTTTTTGTAGTACGAAATCGCTGCTATAAAt   <  1:6239/250‑1 (MQ=255)                                                                                                                                                                                                                                            cgcCGAATGCCGGCACACCCAATTTGCCGTAAATAAAAATATAATTAAGAGGAATATTGATCAGCAAGCCGAGAAAGGTGATCACCATCGCTGGTTTAGTTTTCGCGATGCCGTCATTTAAACAACGAAAATTCACCATCAACAAATAGCCCGGCAAGCCCCAAACCATGGCGTGCAAATAACCGATAGTAATGTCCGCCAAGCGTTGTTCCATGTCCATTTTTTGTAGTACGAAATCGCTGCTATAAATc  <  1:485086/251‑1 (MQ=255)                                                                                                                                                                                                                                            cgcCGAATGCCGGCACACCCAATTTGCCGTAAATAAAAATATAATTAAGAGGAATATTGATCAGCAAGCCGAGAAAGGTGATCACCATCGCTGGTTTAGTTTTCGCGATGCCGTCATTTAAACAACGAAAATTCACCATCAACAAATAGCCCGGCAAGCCCCAAACCATGGCGTGCAAATAACCGATAGTAATGTCCGCCAAGCGTTGTTCCATGTCCATTTTTTGTAGTACGAAATCGCTGCTATAAATc  <  1:826190/251‑1 (MQ=255)                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                               CCCAAAGGCGATAAGAATAAAGAGGTTAGCGCAAACAATGCTACTTCACAGCACAACGCCATGGCAATGGGAAAACCCAGCTTCAGCAATTTCCCCAGCGTGCGTCTATTCGGGCGTTCAATGATATTTGCGAAAACCTTCAAATCACGTTGATTTTTCGCCCGCATACAGTATGCCAACATCAACAGACACATCACCCAGTTTACAATTGCCGTGGCAATGCCACAGCCCACGGCGCCGAATGCCGACACACCCAATTTGCCATAAATAAAAATATAATTAAGAGGAATATTGATCAGCAAGCCGAGAAAGGTGATCACCATCGCTGGTTTAGTTTTCGCGATGCCGTCATTTAAACAACGAAAATTCACCATCAACAAATAGCCCGGCAAGCCCCAAACCATGGCGTGCAAATAACCGATAGTAATGTCCGCCAAGCGTTGTTCCATGTCCATTTTTTGTAGTACGAAATCGCTGCTATAAATC  >  1/1586965‑1587450

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑