Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,581,951	G→A	intergenic (‑137323/‑59813)	unknown ← / → unknown	tRNA‑Met‑CAT/tRNA‑Gly‑GCC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,581,951	0	G	A	100.0%	93.5 / NA	36	intergenic (‑137323/‑59813)	unknown/unknown	tRNA‑Met‑CAT/tRNA‑Gly‑GCC
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (21/15);  total (21/15)

GCTCTGCACCAATAAACTTTCGGTCACTTTTTTTAACATAGAAATCTCGGTCATTTTATCGATAAAAAATTGGTCGGGATTATAACGTATTTTCCGCCAAAACCGAAAAGGAAAGTTACCTGAAAATGGCAATAAATCCGATATTTTCCAAAGAAAAAGAGCGGTTTATCCGCAATTTATTTAAGGGTATAAATTCTATTTTGTTGAATTGTGGAGGGGAATAAAAAGTGCGGTGAATTTTTAATTTGTTTTTTAACCGCACTTTTAATAACTTTTTTTTAAGAATTTCTGCGTTGTGTTTTTTAACTATTT‑AAAAAAAGTGCGGTCATTTTTTTAAGTGTTTTTGTTCTGCATCCGCTCAATCACCACTTTAATATCTTCCGGCGGTGCATAGTAAGGCGCGGAGATGATAAATAAATTTATCCCTAACTTGGCATAATCTGCTATGTTGTTTTTATTCACCCCG  >  1/1581722‑1582187                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                              gCTCTGCACCAATAAACTTTCGGTCACTTTTTTTAACATAGAAATCTCGGTCATTTTATCGATAAAAAATTGGTCGGGATTATAACGTATTTTCCGCCAAAACCGAAAAGGAAAGTTACCTGAAAATGGCAATAAATCCGATATTTTCCAAAGAAAAAGAGCGGTTTATCCGCAATTTATTTAAGGGTATAAATTCTATTTTGTTGAATTGTGGAGGGGAATAAAAAGTACGGTGAATTTTTAATTTGttt                                                                                                                                                                                                                          >  1:336870/1‑251 (MQ=255)       cACCAATAAACTTTCGGTCACTTTTTTTAACATAGAAATCTCGGTCATTTTATCGATAAAAAATTGGTCGGGATTATAACGTATTTTCCGCCAAAACCGAAAAGGAAAGTTACCTGAAAATGGCAATAAATCCGATATTTTCCAAAGAAAAAGAGCGGTTTATCCGCAATTTATTTAAGGGTATAAATTCTATTTTGTTGAATTGTGGAGGGGAATAAAAAGTACGGTGAATTTTTAATTTGTTTTTTAAc                                                                                                                                                                                                                    <  1:879099/251‑1 (MQ=255)                 cTTTCGGTCACTTTTTTTAACATAGAAATCTCGGTCATTTTATCGATAAAAAATTGGTCGGGATTATAACGTATTTTCCGCCAAAACCGAAAAGGAAAGTTACCTGAAAATGGCAATAAATCCGATATTTTCCAAAGAAAAAGAGCGGTTTATCCGCAATTTATTTAAGGGTATAAATTCTATTTTGTTGAATTGTGGAGGGGAATAAAAAGTACGGTGAATTTTTAATTTGTTTTTTAACCGCACTTTta                                                                                                                                                                                                          >  1:361900/1‑251 (MQ=255)                                    aCATAGAAATCTCGGTCATTTTATCGATAAAAAATTGGTCGGGATTATAACGTATTTTCCGCCAAAACCGAAAAGGAAAGTTACCTGAAAATGGCAATAAATCCGATATTTTCCAAAGAAAAAGAGCGGTTTATCCGCAATTTATTTAAGGGTATAAATTCTATTTTGTTGAATTGTGGAGGGGAATAAAAAGTACGGTGAATTTTTAATTTGTTTTTTAACCGCACTTTTAATAACTTTTTTTTAAGAAt                                                                                                                                                                                       >  1:323583/1‑251 (MQ=255)                                          aaaTCTCGGTCATTTTATCGATAAAAAATTGGTCGGGATTATAACGTATTTTCCGCCAAAACCGAAAAGGAAAGTTACCTGAAAATGGCAATAAATCCGATATTTTCCAAAGAAAAAGAGCGGTTTATCCGCAATTTATTTAAGGGTATAAATTCTATTTTGTTGAATTGTGGAGGGGAATAAAAAGTACGGTGAATTTTTAATTTGTTTTTTAACCGCACTTTTAATAACTTTTTTTTAAGAATTTCTGc                                                                                                                                                                                 <  1:861815/251‑1 (MQ=255)                                                                             ggATTATAACGTATTTTCCGCCAAAACCGAAAAGGAAAGTTACCTGAAAATGGCAATAAATCCGATATTTTCCAAAGAAAAAGAGCGGTTTATCCGCAATTTATTTAAGGGTATAAATTCTATTTTGTTGAATTGTGGAGGGGAATAAAAAGTACGGTGAATTTTTAATTTGTTTTTTAACCGCACTTTTAATAACTTTTTTTTAAGAATTTCTGCGTTGTGTTTTTTAACTATTTAAAAAAAAGTGCGGt                                                                                                                                              >  1:552089/1‑251 (MQ=255)                                                                                                                      aCCTGAAAATGGCAATAAATCCGATATTTTCCAAAGAAAAAGAGCGGTTTATCCGCAATTTATTTAAGGGTATAAATTCTATTTTGTTGAATTGTGGAGGGGAATAAAAAGTACGGTGAATTTTTAATTTGTTTTTTAACCGCACTTTTAATAACTTTTTTTTAAGAATTTCTGCGTTGTGTTTTTTAACTATTT‑aaaaa                                                                                                                                                       <  1:771693/200‑1 (MQ=255)                                                                                                                                       aaTCCGATATTTTCCAAAGAAAAAGAGCGGTTTATCCGCAATTTATTTAAGGGTATAAATTCTATTTTGTTGAATTGTGGAGGGGAATAAAAAGTACGGTGAATTTTTAATTTGTTTTTTAACCGCACTTTTAATAACTTTTTTTTAAGAATTTCTGCGTTGTGTTTTTTAACTATTTAAAAAAAAGTGCGGTCATTTTTTTAAGTGTTTTTGTTCTGCATCCGCTCAATCACCACTTTAATATCTTCcgg                                                                                    <  1:825050/251‑1 (MQ=255)                                                                                                                                             atatTTTCCAAAGAAAAAGAGCGGTTTATCCGCAATTTATTTAAGGGTATAAATTCTATTTTGTTGAATTGTGGAGGGGAATAAAAAGTACGGTGAATTTTTAATTTGTTTTTTAACCGCACTTTTAATAACTTTTTTTTAAGAATTTCTGCGTTGTGTTTTTTAACTATTTAAAAAAAAGTGCGGTCATTTTTTTAAGTGTTTTTGTTCTGCATCCGCTCAATCACCACTTTAATATCTTCCGGCGGTGc                                                                              <  1:678244/251‑1 (MQ=255)                                                                                                                                                      aaaGAAAAAGAGCGGTTTATCCGCAATTTATTTAAGGGTATAAATTCTATTTTGTTGAATTGTGGAGGGGAATAAAAAGTACGGTGAATTTTTAATTTGTTTTTTAACCGCACTTTTAATAACTTTTTTTTAAGAATTTCTGCGTTGTGTTTTTTAACTATTTAAAAAAAAGTGCGGTCATTTTTTTAAGTGTTTTTGTTCTGCATCCGCTCAATCACCACTTTAATATCTTCCGGCGGTGCATAGTAAgg                                                                     <  1:169977/251‑1 (MQ=255)                                                                                                                                                         gAAAAAGAGCGGTTTATCCGCAATTTATTTAAGGGTATAAATTCTATTTTGTTGAATTGTGGAGGGGAATAAAAAGTACGGTGAATTTTTAATTTGTTTTTTAACCGCACTTTTAATAACTTTTTTTTAAGAATTTCTGCGTTGTGTTTTTTAACTATTTAAAAAAAAGTGCGGTCATTTTTTTAAGTGTTTTTGTTCTGCATCCGCTCAATCACCACTTTAATATCTTCCGGCGGTGCATAGTAAGgcg                                                                   >  1:723930/1‑250 (MQ=255)                                                                                                                                                          aaaaaGAGCGGTTTATCCGCAATTTATTTAAGGGTATAAATTCTATTTTGTTGAATTGTGGAGGGGAATAAAAAGTACGGTGAATTTTTAATTTGTTTTTTAACCGCACTTTTAATAACTTTTTTTTAAGAATTTCTGCGTTGTGTTTTTTAACTATTTAAAAAAAAGTGCGGTCATTTTTTTAAGTGTTTTTGTTCTGCATCCGCTCAATCACCACTTTAATATCTTCCGGCGGTGCATAGTAAGgcgc                                                                  <  1:29537/250‑1 (MQ=255)                                                                                                                                                                    gTTTATCCGCAATTTATTTAAGGGTATAAATTCTATTTTGTTGAATTGTGGAGGGGAATAAAAAGTACGGTGAATTTTTAATTTGTTTTTTAACCGCACTTTTAATAACTTTTTTTTAAGAATTTCTGCGTTGTGTTTTTTAACTATTTAAAAAAAAGTGCGGTCATTTTTTTAAGTGTTTTTGTTCTGCATCCGCTCAATCACCACTTTAATATCTTCcggcgg                                                                                 <  1:342164/225‑1 (MQ=255)                                                                                                                                                                                   atttaAGGGTATAAATTCTATTTTGTTGAATTGTGGAGGGGAATAAAAAGTACGGTGAATTTTTAATTTGTTTTTTAACCGCACTTTTAATAACTTTTTTTTAAGAATTTCTGCGTTGTGTTTTTTAACTATTTAAAAAAAAGTGCGGTCATTTTTTTAAGTGTTTTTGTTCTGCATCCGCTCAATCACCACTTTAatat                                                                                           >  1:702181/1‑200 (MQ=255)                                                                                                                                                                                   atttaAGGGTATAAATTCTATTTTGTTGAATTGTGGAGGGGAATAAAAAGTACGGTGAATTTTTAATTTGTTTTTTAACCGCACTTTTAATAACTTTTTTTTAAGAATTTCTGCGTTGTGTTTTTTAACTATTTAAAAAAAAGTGCGGTCATTTTTTTAAGTGTTTTTGTTCTGCATCCGCTCAATCACCACTTTAATATCTTCCGGCGGTGCATAGTAAGGCGCGGAGATGATAAATAAATTTATCCCTa                                        >  1:57777/1‑251 (MQ=255)                                                                                                                                                                                         gggTATAAATTCTATTTTGTTGAATTGTGGAGGGGAATAAAAAGTACGGTGAATTTTTAATTTGTTTTTTAACCGCACTTTTAATAACTTTTTTTTAAGAATTTCTGCGTTGTGTTTTTTAACTATTTAAAAAAAAGTGCGGTCATTTTTTTAAGTGTTTTTGTTCTGCATCCGCTCAATCACCACTTTAatat                                                                                           <  1:479488/194‑1 (MQ=255)                                                                                                                                                                                         gggTATAAATTCTATTTTGTTGAATTGTGGAGGGGAATAAAAAGTACGGTGAATTTTTAATTTGTTTTTTAACCGCACTTTTAATAACTTTTTTTTAAGAATTTCTGCGTTGTGTTTTTTAACTATTTAAAAAAAAGTGCGGTCATTTTTTTAAGTGTTTTTGTTCTGCATCCGCTC‑ATCAACAATTTAATATCCTTCGGCGGGGCCTTGTTAGGCCGGGAGATGATAAATAAATTTATCCCTAACTTgg                                  >  1:699015/1‑250 (MQ=255)                                                                                                                                                                                          ggTATAAATTCTATTTTGTTGAATTGTGGAGGGGAATAAAAAGTACGGTGAATTTTTAATTTGTTTTTTAACCGCACTTTTAATAACTTTTTTTTAAGAATTTCTGCGTTGTGTTTTTTAACTATTTAAAAAAAAGTGCGGTCATTTTTTTAAGTGTTTTTGTTCTGCATCCGCTCAATCACCACTTTAATATCTTCCGGCGGTGCATTGTAAGGCGCGGAGATGATAAATAAATTTATCCCTAACTTgg                                  >  1:644628/1‑250 (MQ=255)                                                                                                                                                                                           gTATAAATTCTATTTTGTTGAATTGTGGAGGGGAATAAAAAGTACGGTGAATTTTTAATTTGTTTTTTAACCGCACTTTTAATAACTTTTTTTTAAGAATTTCTGCGTTGTGTTTTTTAACTATTTAAAAAAAAGTGCGGTCATTTTTTTAAGTGTTTTTGTTCTGCATCCGCTCAATCACCACTTTAATATCTTCCGGCGGTGCATAGTAAGgcgc                                                                  >  1:488041/1‑217 (MQ=255)                                                                                                                                                                                               aaaTTCTATTTTGTTGAATTGTGGAGGGGAATAAAAAGTACGGTGAATTTTTAATTTGTTTTTTAACCGCACTTTtaat                                                                                                                                                                                                        <  1:362780/79‑1 (MQ=255)                                                                                                                                                                                               aaaTTCTATTTTGTTGAATTGTGGAGGGGAATAAAAAGTACGGTGAATTTTTAATTTGTTTTTTAACCGCACTTTTAATAACTTTTTTTTAAGAATTTCTGCGTTGTGTTTTTTAACTATTTAAAAAAAAGTGCGGTCATTTTTTTAAGTGTTTTTGTTCTGCATCCGCTCAATCACCACTTTAATATCTTCCGGCGGTGCATAGTAAGGCGCGGAGATGATAAATAAATTTATCCCTAACTTGGCATAAt                            >  1:214345/1‑251 (MQ=255)                                                                                                                                                                                                   tCTATTTTGTTGAATTGTGGAGGGGAATAAAAAGTACGGTGAATTTTTAATTTGTTTTTTAACCGCACTTTTAATAACTTTTTTTTAAGAATTTCTGCGTTGTGTTTTTTAACTATTTAAAAAAAAGTGCGGTCATTTTTTTAAGTGTTTTTGTTCTGCATCCGCTCAATCACCACTTTAATATCTTCCGGCGGTGCATAGTAAGGCGCGGAGATGATAAATAAATTTATCCCTAACTTGGCATAATCTGc                        <  1:278401/251‑1 (MQ=255)                                                                                                                                                                                                         ttgttgAATTGTGGAGGGGAATAAAAAGTACGGTGAATTTTTAATTTGTTTTTTAACCGCACTTTTAATAACTTTTTTTTAAGAATTTCTGCGTTGTGTTTTTTAACTATTTAAAAAAAAGTGCGGTCATTTTTTTAAGTGTTTTTGTTCTGCATCCGCTCAATCACCACTTTAATATCTTCCGGCGGTGCATAGTaa                                                                       <  1:9838/198‑1 (MQ=255)                                                                                                                                                                                                           gttgAATTGTGGAGGGGAATAAAAAGTACGGTGAATTTTTAATTTGTTTTTTAACCGCACTTTTAATAACTTTTTTTTAAGAATTTCTGCGTTGTGTTTTTTAACTATTTAAAAAAAAGTGCGGTCATTTTTTTAAGTGTTTTTGTTCTGCATCCGCTCAATCACCACTTTAATATCTTCCGGCGGTGCATAGTAAGGCGCGGAGATGATAAATAAATTTATCCCTAACTTGGCATAATCTGCTAtgttgt                >  1:828359/1‑251 (MQ=255)                                                                                                                                                                                                                aTTGTGGAGGGGAATAAAAAGTACGGTGAATTTTTAATTTGTTTTTTAACCGCACTTTTAATAACTTTTTTTTAAGAATTTCTGCGTTGTGTTTTTTAACTATTTAAAAAAAAGTGCGGTCATTTTTTTAAGTGTTTTTGTTCTGCATCCGCTCAATCACCACTTTAATATCTTCCGGCGGTGCATAGTAAGGCGCGGAGATGATAAATAAATTTATCCCTAACTTGGCATAATCTGCTATGTTGTTTTTa           >  1:844050/1‑251 (MQ=255)                                                                                                                                                                                                                 ttGTGGAGGGGAATAAAAAGTACGGTGAATTTTTAATTTGTTTTTTAACCGCACTTTTAATAACTTTTTTTTAAGAATTTCTGCGTTGTGTTTTTTAACTATTTAAAAAAAAGTGCGGTCATTTTTTTAAGTGTTTTTGTTCTGCATCCGCTCAATCACCACTTTAATATCTTCCGGCGGTGCATAGTAAGGCGCGGAGATGata                                                        >  1:885191/1‑205 (MQ=255)                                                                                                                                                                                                                   gtgGAGGGGAATAAAAAGTACGGTGAATTTTTAATTTGTTTTTTAACCGCACTTTTAATAACTTTTTTTTAAGAATTTCTGCGTTGTGTTTTTTAACTATTTAAAAAAAAGTGCGGTCATTTTTTTAAGTGTTTTTGTTCTGCATCCGCTCAATCACCACTTTAATATCTTCCGGCGGTGCATAGTAAgg                                                                     >  1:203827/1‑190 (MQ=255)                                                                                                                                                                                                                      gAGGGGAATAAAAAGTACGGTGAATTTTTAATTTGTTTTTTAACCGCACTTTTAATAACTTTTTTTTAAGAATTTCTGCGTTGTGTTTTTTAACTATTTAAAAAAAAGTGCGGTCATTTTTTTAAGTGTTTTTGTTCTGCATCCGCTCAATCACCACTTTAatat                                                                                           <  1:750574/165‑1 (MQ=255)                                                                                                                                                                                                                        ggggAATAAAAAGTACGGTGAATTTTTAATTTGTTTTTTAACCGCACTTTTAATAACTTTTTTTTAAGAATTTCTGCGTTGTGTTTTTTAACTATTTAAAAAAAAGTGCGGTCATTTTTTTAAGTGTTTTTGTTCTGCATCCGCTCAATCACCACTTTAATATCTTCCGGCGGTGCATAGTAAGGCGCGGAGATGATAAATAAATTTATCCCTAACTTGGCATAATCTGc                        >  1:422546/1‑230 (MQ=255)                                                                                                                                                                                                                          ggAATAAAAAGTACGGTGAATTTTTAATTTGTTTTTTAACCGCACTTTTAATAACTTTTTTTTAAGAATTTCTGCGTTGTGTTTTTTAACTATTTAAAAAAAAGTGCGGTCATTTTTTTAAGTGTTTTTGTTCTGCATCCGCTCAATCACCACTTTAatat                                                                                           <  1:392120/161‑1 (MQ=255)                                                                                                                                                                                                                          ggAATAAAAAGTACGGTGAATTTTTAATTTGTTTTTTAACCGCACTTTTAATAACTTTTTTTTAAGAATTTCTGCGTTGTGTTTTTTAACTATTTAAAAAAAAGTGCGGTCATTTTTTTAAGTGTTTTTGTTCTGCATCCGCTCAATCACCACTTTAATATCTTCCGGCGGTGCATAGTAAGGCGCGGAGATGATAAATAAATTTATCCCTAACTTGGCATAATCTGCTATGTTGTTTTTATTCACCccg  >  1:501845/1‑250 (MQ=255)                                                                                                                                                                                                                          ggAATAAAAAGTACGGTGAATTTTTAATTTGTTTTTTAACCGCACTTTTAATAACTTTTTTTTAAGAATTTCTGCGTTGTGTTTTTTAACTATTTAAAAAAAAGTGCGGTCATTTTTTTAAGTGTTTTTGTTCTGCATCCGCTCAATCACCACTTTAATATCTTCCGGCGGTGCATAGTAAGGCGCGGAGATGATAAATAAATTTATCCCTAACTTGGCATAATCTGCTATGTTGTTTTTATTCACCccg  >  1:174574/1‑250 (MQ=255)                                                                                                                                                                                                                           gAATAAAAAGTACGGTGAATTTTTAATTTGTTTTTTAACCGCACTTTTAATAACTTTTTTTTAAGAATTTCTGCGTTGTGTTTTTTAACTATTTAAAAAAAAGTGCGGTCATTTTTTTAAGTGTTTTTGTTCTGCATCCGCTCAATCACCACTTTAATATCTTCCGGCGGTGCATAGTAAgg                                                                     <  1:510752/182‑1 (MQ=255)                                                                                                                                                                                                                           gAATAAAAAGTACGGTGAATTTTTAATTTGTTTTTTAACCGCACTTTTAATAACTTTTTTTTAAGAATTTCTGCGTTGTGTTTTTTAACTATTTAAAAAAAAGTGCGGTCATTTTTTTAAGTGTTTTTGTTCTGCATCCGCTCAATCACCACTTTAATATCTTCCGGCGGTGCATAGTAAGGCGCGGAGATGATAAATAAATTTATCCCTAACTTGGCATAATCTGCTAtgt                   >  1:174160/1‑232 (MQ=255)                                                                                                                                                                                                                              tAAAAAGTACGGTGAATTTTTAATTTGTTTTTTAACCGCACTTTTAATAACTTTTTTTTAAGAATTTCTGCGTTGTGTTTTTTAACTATTTAAAAAAAAGTGCGGTCATTTTTTTAAGTGTTTTTGTTCTGCATCCGCTCAATCACCACTTTAatat                                                                                           >  1:800536/1‑157 (MQ=255)                                                                                                                                                                                                                                  aaGTACGGTGAATTTTTAATTTGTTTTTTAACCGCACTTTTAATAACTTTTTTTTAAGAATTTCTGCGTTGTGTTTTTTAACTATTTAAAAAAAAGTGCGGTCATTTTTTTAAGTGTTTTTGTTCTGCATCCGCTCAATCACCACTTTAATATCTTCCGGCGGTGCATAGTa                                                                        >  1:277136/1‑172 (MQ=255)                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                              GCTCTGCACCAATAAACTTTCGGTCACTTTTTTTAACATAGAAATCTCGGTCATTTTATCGATAAAAAATTGGTCGGGATTATAACGTATTTTCCGCCAAAACCGAAAAGGAAAGTTACCTGAAAATGGCAATAAATCCGATATTTTCCAAAGAAAAAGAGCGGTTTATCCGCAATTTATTTAAGGGTATAAATTCTATTTTGTTGAATTGTGGAGGGGAATAAAAAGTGCGGTGAATTTTTAATTTGTTTTTTAACCGCACTTTTAATAACTTTTTTTTAAGAATTTCTGCGTTGTGTTTTTTAACTATTT‑AAAAAAAGTGCGGTCATTTTTTTAAGTGTTTTTGTTCTGCATCCGCTCAATCACCACTTTAATATCTTCCGGCGGTGCATAGTAAGGCGCGGAGATGATAAATAAATTTATCCCTAACTTGGCATAATCTGCTATGTTGTTTTTATTCACCCCG  >  1/1581722‑1582187

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑