Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,573,299	C→A	intergenic (‑128671/‑68465)	unknown ← / → unknown	tRNA‑Met‑CAT/tRNA‑Gly‑GCC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,573,299	0	C	A	100.0%	127.5 / NA	48	intergenic (‑128671/‑68465)	unknown/unknown	tRNA‑Met‑CAT/tRNA‑Gly‑GCC
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (25/23);  total (25/23)

CGCTGTTTTAACGGAATGTGAGGTAAAAAACGCCCCCCTATGCAGGTAAGCAATAAGCCGCATAGAACAAAATAAAGACTTTTACGGGATATCTGTGACATAAAATCAATTATGGATAAAAGCACTCAGAAAAGTGCGGTATGTTCCTGATGTATTTTAATAAGATATTAAAAATAATAATTTACTTTTGAATTAAACAACCCACAACCAACTCACTTTTCTACAACGAGAAAAAGAGAGCTGCTTCATAGTTCAAAAAGAATCCGCCGACGATTAACCAAATATAAATCAATGTGCCGAGAATTAACGGTTTTAATCCTGCCTGTTTAATTGCGCCGAGGTGTGTAGTTAATCCTAATGCCGCCATCGCAGTAACGAGTAACAAAGAATCCAGTTCGACTAAAAGTTTCACTAAAAAATCCGGCAATAAATTGAAAGAATTGAATACCGCAACCGCA  >  1/1573088‑1573545                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                       cGCTGTTTTAACGGAATGTGAGGTAAAAAACGCCCCCCTATGCAGGTAAGCAATAAGCCGCATAGAACAAAATAAAGACTTTTACGGGATATCTGTGACATAAAATCAATTATGGATAAAAGCACTCAGAAAAGTGCGGTATGTTCCTGATGTATTTTAATAAGATATTAAAAATAATAATTTACTTTTGAATTAAACAACCCACAACCAAATCACTTTTCTACAACGAGAAAAAGAGAGCTGCTTCATAg                                                                                                                                                                                                                 <  1:661564/251‑1 (MQ=255)          aaCGGAATGTGAGGTAAAAAACGCCCCCCTATGCAGGTAAGCAATAAGCCGCATAGAACAAAATAAAGACTTTTACGGGATATCTGTGACATAAAATCAATTATGGATAAAAGCACTCAGAAAAGTGCGGTATGTTCCTGATGTATTTTAATAAGATATTAAAAATAATAATTTACTTTTGAATTAAACAACCCACAACCAAATCACTTTTCTACAACGAGAAAAAGAGAGCTGCTTCATAGTTCAAAAAg                                                                                                                                                                                                        <  1:541683/251‑1 (MQ=255)                        tAAAAAACGCCCCCCTATGCAGGTAAGCAATAAGCCGCATAGAACAAAATAAAGACTTTTACGGGATATCTGTGACATAAAATCAATTATGGATAAAAGCACTCAGAAAAGTGCGGTATGTTCCTGATGTATTTTAATAAGATATTAAAAATAATAATTTACTTTTGAATTAAACAACCCACAACCAAATCACTTTTCTACAACGAGAAAAAGAGAGCTGCTTCATAGTTCAAAAAGAATCCGCCGACGAt                                                                                                                                                                                          <  1:613258/251‑1 (MQ=255)                             aaCGCCCCCCTATGCAGGTAAGCAATAAGCCGCATAGAACAAAATAAAGACTTTTACGGGATATCTGTGACATAAAATCAATTATGGATAAAAGCACTCAGAAAAGTGCGGTATGTTCCTGATGTATTTTAATAAGATATTAAAAATAATAATTTACTTTTGAATTAAACAACCCACAACCAAATCACTTTTCTACAACGAGAAAAAGAGAGCTGCTTCATAGTTCAAAAAGAATCCGCCGACGATTAAcc                                                                                                                                                                                     <  1:391287/251‑1 (MQ=255)                                   ccccTATGCAGGTAAGCAATAAGCCGCATAGAACAAAATAAAGACTTTTACGGGATATCTGTGACATAAAATCAATTATGGATAAAAGCACTCAGAAAAGTGCGGTATGTTCCTGATGTATTTTAATAAGATATTAAAAATAATAATTTACTTTTGAATTAAACAACCCACAACCAAATCACTTTTCTAc                                                                                                                                                                                                                                            <  1:362173/190‑1 (MQ=255)                                   ccccTATGCAGGTAAGCAATAAGCCGCATAGAACAAAATAAAGACTTTTACGGGATATCTGTGACATAAAATCAATTATGGATAAAAGCACTCAGAAAAGTGCGGTATGTTCCTGATGTATTTTAATAAGATATTAAAAATAATAATTTACTTTTGAATTAAACAACCCACAACCAAATCACTTTTCTACAACGAGAAAAAGAGAGCTGCTTCATAg                                                                                                                                                                                                                 <  1:349563/217‑1 (MQ=255)                                                  gCAATAAGCCGCATAGAACAAAATAAAGACTTTTACGGGATATCTGTGACATAAAATCAATTATGGATAAAAGCACTCAGAAAAGTGCGGTATGTTCCTGATGTATTTTAATAAGATATTAAAAATAATAATTTACTTTTGAATTAAACAACCCACAACCAAATCACTTTTCTACAACGAGAAAAAGAGAGCTGCTTCATAGTTCAAAAAGAATCCGCCGACGATTAACCAAATATAAATCAATGTGCCga                                                                                                                                                                >  1:148505/1‑251 (MQ=255)                                                  gCAATAAGCCGCATAGAACAAAATAAAGACTTTTACGGGATATCTGTGACATAAAATCAATTATGGATAAAAGCACTCAGAAAAGTGCGGTATGTTCCTGATGTATTTTAATAAGATATTAAAAATAATAATTTACTTTTGAATTAAACAACCCACAACCAAATCACTTTTCTACAACGAGAAAAAGAGAGCTGCTTCATAGTTCAAAAAGAATCCGCCGACGATTAACCAAATATAAATCAATGTGCCga                                                                                                                                                                >  1:426160/1‑251 (MQ=255)                                                  gCAATAAGCCGCATAGAACAAAATAAAGACTTTTACGGGATATCTGTGACATAAAATCAATTATGGATAAAAGCACTCAGAAAAGTGCGGTATGTTCCTGATGTATTTTAATAAGATATTAAAAATAATAATTTACTTTTGAATTAAACAACCCACAACCAAATCACTTTTCTACAACGAGAAAAAGAGAGCTGCTTCATAGTTCAAAAAGAATCCGCCGACGATTAACCAAATATAAATCAATGTGCCga                                                                                                                                                                >  1:515691/1‑251 (MQ=255)                                                  gCAATAAGCCGCATAGAACAAAATAAAGACTTTTACGGGATATCTGTGACATAAAATCAATTATGGATAAAAGCACTCAGAAAAGTGCGGTATGTTCCTGATGTATTTTAATAAGATATTAAAAATAATAATTTACTTTTGAATTAAACAACCCACAACCAAATCACTTTTCTACAACGAGAAAAAGAGAGCTGCTTCATAGTTCAAAAAGAATCCGCCGACGATTAACCAAATATAAATCAATGTGCCga                                                                                                                                                                >  1:545892/1‑251 (MQ=255)                                                             cATAGAACAAAATAAAGACTTTTACGGGATATCTGTGACATAAAATCAATTATGGATAAAAGCACTCAGAAAAGTGCGGTATGTTCCTGATGTATTTTAATAAGATATTAAAAATAATAATTTACTTTTGAATTAAACAACCCACAACCAAATCACTTTTCTACAACGAGAAAAAGAGAGCTGCTTCATAGTTCAAAAAGAATCCGCCGACGATTAACCAAata                                                                                                                                                                                >  1:730281/1‑224 (MQ=255)                                                                    cAAAATAAAGACTTTTACGGGATATCTGTGACATAAAATCAATTATGGATAAAAGCACTCAGAAAAGTGCGGTATGTTCCTGATGTATTTTAATAAGATATTAAAAATAATAATTTACTTTTGAATTAAACAACCCACAACCAAATCACTTTTCTACAACGAGAAAAAGAGAGCTGCTTCATAGTTCAAAAAGAATCCGCCGACGATTAACCAAATATAAATCAATGTGCCGAGAATTAACGGTTTTAATc                                                                                                                                              <  1:405969/251‑1 (MQ=255)                                                                               cTTTTACGGGATATCTGTGACATAAAATCAATTATGGATAAAAGCACTCAGAAAAGTGCGGTATGTTCCTGATGTATTTTAATAAGATATTAAAAATAATAATTTACTTTTGAATTAAACAACCCACAACCAAATCACTTTTCTACAACGAGAAAAAGAGAGCTGCTTCATAGTTCAAAAAGAATCCGCCGACGATTAACCAAatat                                                                                                                                                                               <  1:706799/207‑1 (MQ=255)                                                                                 tttACGGGATATCTGTGACATAAAATCAATTATGGATAAAAGCACTCAGAAAAGTGCGGTATGTTCCTGATGTATTTTAATAAGATATTAAAAATAATAATTTACTTTTGAATTAAACAACCCACAACCAAATCACTTTTCTACAACGAGAAAAAGAGAGCTGCTTCATAGTTCAAAAAGAATCCGCCGACGATTAACCAAATATAAATCAATGTGCCGAGAATTAACGGTTTTAATCCTGCCTGTTTAAt                                                                                                                                 <  1:747002/251‑1 (MQ=255)                                                                                   tACGGGATATCTGTGACATAAAATCAATTATGGATAAAAGCACTCAGAAAAGTGCGGTATGTTCCTGATGTATTTTAATAAGATATTAAAAATAATAATTTACTTTTGAATTAAACAACCCACAACCAAATCACTTTTCTACAACGAGAAAAAGAGAGCTGCTTCATAGTTCAAAAAGAATCCGCCGACGATTAACCAAATATAAATCAATGTGCCGAGAATTAACGGTTTTAATCCTGCCTGTTTAATTg                                                                                                                               <  1:641367/251‑1 (MQ=255)                                                                                               gtgACATAAAATCAATTATGGATAAAAGCACTCAGAAAAGTGCGGTATGTTCCTGATGTATTTTAATAAGATATTAAAAATAATAATTTACTTTTGAATTAAACAACCCACAACCAAATCACTTTTCTACAACGAGAAAAAGAGAGCTGCTTCATAGTTCAAAAAGAATCCGCCGACGATTAAcc                                                                                                                                                                                     >  1:175894/1‑185 (MQ=255)                                                                                                  aCATAAAATCAATTATGGATAAAAGCACTCAGAAAAGTGCGGTATGTTCCTGATGTATTTTAATAAGATATTAAAAATAATAATTTACTTTTGAATTAAACAACCCACAACCAAATCACTTTTCTACAACGAGAAAAagag                                                                                                                                                                                                                              >  1:2587/1‑141 (MQ=255)                                                                                                   cATAAAATCAATTATGGATAAAAGCACTCAGAAAAGTGCGGTATGTTCCTGATGTATTTTAATAAGATATTAAAAATAATAATTTACTTTTGAATTAAACAACCCACAACCAAATCACTTTTCTACAACGAGAAAAAGAGAGCTGCTTCATAGTTCAAAAAGAATCCGCCGACGATTAACCAAATATAAATCAATGTGCCGAGAATTAACGGTTTTAATCCTGCCTGTTTAATTGCGCCGAGGTGTGTAGt                                                                                                               <  1:609514/251‑1 (MQ=255)                                                                                                         aTCAATTATGGATAAAAGCACTCAGAAAAGTGCGGTATGTTCCTGATGTATTTTAATAAGATATTAAAAATAATAATTTACTTTTGAATTAAACAACCCACAACCAAATCACTTTTCTACAACGAGAAAAAGAGAGCTGCTTCATAGTTCAAAAAGAATCCGCCGACGATTAACCAAATATAAATCAATGTGCCGAGAATTAACGGTTTTAATCCTGCCTGTTTAATTGCGCCGAGGTGTGTAGTTAATcc                                                                                                         <  1:4324/251‑1 (MQ=255)                                                                                                                            aCTCAGAAAAGTGCGGTATGTTCCTGATGTATTTTAATAAGATATTAAAAATAATAATTTACTTTTGAATTAAACAACCCACAACCAAATCACTTTTCTACAACGAGAAAAAGAGAGCTGCTTCATAGTTCAAGAAGAATCCGCCGACGATTAACCAAATATAAATCAATGTGCCGAGAATTAACGGTTTTAATCCTGCCTGTTTAATTGCGCCGAGGTGTGTAGTTAATCCTAATGCCGCCATCGCAGTa                                                                                      >  1:404748/1‑251 (MQ=255)                                                                                                                              tCAGAAAAGTGCGGTATGTTCCTGATGTATTTTAATAAGATATTAAAAATAATAATTTACTTTTGAATTAAACAACCCACAACCAAATCACTTTTCTACAACGAGAAAAAGAGAGCTGCTTCATAGTTCAAAAAGAATCCGCCGACGATTAACCAAATATAAATCAATGTGCCGAGAATTAACGGTTTTAATCCTGCCTGTTTAATTGCGCCGAGGTGTGTAGTTAATCCTAATGCCGCCATCGCAGTAAc                                                                                    <  1:214049/251‑1 (MQ=255)                                                                                                                                 gAAAAGTGCGGTATGTTCCTGATGTATTTTAATAAGATATTAAAAATAATAATTTACTTTTGAATTAAACAACCCACAACCAAATCACTTTTCTACAACGAGAAAAAGAGAGCTGCTTCATAGTTCAAAAAGAATCCGCCGACGATTAACCAAATATAAATCAATGTGCCGAGAATTAACGGTTTTAATCCTGCCTGTTTAATTGCGCCGAGGTGTGTAGTTAATCCTAATGCCGCCATCGCAGTAACGAg                                                                                 >  1:777402/1‑251 (MQ=255)                                                                                                                                           gTATGTTCCTGATGTATTTTAATAAGATATTAAAAATAATAATTTACTTTTGAATTAAACAACCCACAACCAAATCACTTTTCTACAACGAGAAAAAGAGAGCTGCTTCATAGTTCAAAAAGAATCCGCCGACGATTAACCAAATATAAATCAATGTGCCGAGAATTAACGGTTTTAATcc                                                                                                                                             <  1:809157/181‑1 (MQ=255)                                                                                                                                           gTATGTTCCTGATGTATTTTAATAAGATATTAAAAATAATAATTTACTTTTGAATTAAACAACCCACAACCAAATCACTTTTCTACAACGAGAAAAAGAGAGCTGCTTCATAGTTCAAAAAGAATCCGCCGACGATTAACCAAATATAAATCAATGTGCCGAGAATTAACGGTTTTAATCCTGCCTGTTTAATTGCGCCGAGGTGTGTAGTTAATCCTAATGCCGCCATCGcctgtctcttatacacacc                                                                        >  1:653277/1‑232 (MQ=255)                                                                                                                                             aTGTTCCTGATGTATTTTAATAAGATATTAAAAATAATAATTTACTTTTGAATTAAACAACCCACAACCAAATCACTTTTCTACAACGAGAAAAAGAGAGCTGCTTCATAg                                                                                                                                                                                                                 <  1:526535/111‑1 (MQ=255)                                                                                                                                             aTGTTCCTGATGTATTTTAATAAGATATTAAAAATAATAATTTACTTTTGAATTAAACAACCCACAACCAAATCACTTTTCTACAACGAGAAAAAGAGAGCTGCTTCATAGTTCAAAAAGAATCCGCCGACGATTAACCAAATATAAATCAATGTGCCGAGAATTAACGGTTTTAATcctgcc                                                                                                                                         >  1:552634/1‑183 (MQ=255)                                                                                                                                               gTTCCTGATGTATTTTAATAAGATATTAAAAATAATAATTTACTTTTGAATTAAACAACCCACAACCAAATCACTTTTCTACAACGAGAAAAAGAGAGCTGCTTCATAGTTCAAAAAGAATCCGCCGACGATTAACCAAATATAAATCAATGTGCCGAGAATTAACGGTTTTAATCCTGCCTGTTTAATTGCGCCGAGGTGTGTAGTTAATCCTAATGCCGCCATCGCAGTAACGAGTAACAAAGAATCCa                                                                   >  1:88499/1‑251 (MQ=255)                                                                                                                                                                ataaGATATTAAAAATAATAATTTACTTTTGAATTAAACAACCCACAACCAAATCACTTTTCTACAACGAGAAAAAGAGAGCTGCTTCATAGTTCAAAAAGAATCCGCCGACGATTAACCAAATATAAATCAATGTGCCGAGAATTAACGGTTTTAATCCTGCCTGTTTAATTGCGCCGAGGTGTGTAGTTAATc                                                                                                          >  1:459255/1‑195 (MQ=255)                                                                                                                                                                    gATATTAAAAATAATAATTTACTTTTGAATTAAACAACCCACAACCAAATCACTTTTCTACAACGAGAAAAAGAGAGCTGCTTCATAGTTCAAAAAGAATCCGCCGACGATTAACCAAATATAAATCAATGTGCCGAGAATTAACGGTTTTAATCCTGCCTGTTTAATTGCGCCGAGGTGTGTAGTTAATCCTAATGCCGCCATCGCAGTAACGAGTAACAAAGAATCCAGTTCGACTAAAAGTTTCACTa                                              >  1:406435/1‑251 (MQ=255)                                                                                                                                                                          aaaaaTAATAATTTACTTTTGAATTAAACAACCCACAACCAAATCACTTTTCTACAACGAGAAAAAGAGAGCTGCTTCATAGTTCAAAAAGAATCCGCCGACGATTAACCAAATATAAATCAATGTGCCGAGAATTAACGGTTTTAATCCTGCCTGTTTAATTGCGCCGAGGTGTGTAGTTAATCCTAATGCCGCCATCGCAGTAACGAGTAACAAAGAATCCAGTTCGACTAAAAGTTTCACTAAAAAAt                                        <  1:783268/251‑1 (MQ=255)                                                                                                                                                                            aaaTAATAATTTACTTTTGAATTAAACAACCCACAACCAAATCACTTTTCTACAACGAGAAAAAGAGAGCTGCTTCATAGTTCAAAAAGAATCCGCCGACGATTAACCAAATATAAATCAATGTGCCGAGAATTAACGGTTTTAATCCTGCCTGTTTAATTGCGCCGAGGTGTGTAGTTAATCCTAATGCCGCCATCGCAGTAACGAGTAACAAAGAAt                                                                      <  1:584313/219‑1 (MQ=255)                                                                                                                                                                            aaaTAATAATTTACTTTTGAATTAAACAACCCACAACCAAATCACTTTTCTACAACGAGAAAAAGAGAGCTGCTTCATAGTTCAAAAAGAATCCGCCGACGATTAACCAAATATAAATCAATGTGCCGAGAATTAACGGTTTTAATCCTGCCTGTTTAATTGCGCCGAGGTGTGTAGTTAATCCTAATGCCGCCATCGCAGTAACGAGTAACAAAGAATCCAGTTCGACTAAAAGTTTCACTAAAAAATc                                       >  1:761129/1‑250 (MQ=255)                                                                                                                                                                             aataataatTTACTTTTGAATTAAACAACCCACAACCAAATCACTTTTCTACAACGAGAAAAAGAGAGCTGCTTCATAGTTCAAAAAGAATCCGCCGACGATTAAcc                                                                                                                                                                                     >  1:492347/1‑107 (MQ=255)                                                                                                                                                                               taataatTTACTTTTGAATTAAACAACCCACAACCAAATCACTTTTCTACAACGAGAAAAAGAGAGCTGCTTCATAGTTCAAAAAGAATCCGCCGACGATTAACCAAATATAAATCAATGTGCCGAGAATTAACGGTTTTAATCCTGCCTGTTTAATTg                                                                                                                               <  1:763918/159‑1 (MQ=255)                                                                                                                                                                               taataatTTACTTTTGAATTAAACAACCCACAACCAAATCACTTTTCTACAACGAGAAAAAGAGAGCTGCTTCATAGTTCAAAAAGAATCCGCCGACGATTAACCAAATATAAATCAATGTGCCGAGAATTAACGGTTTTAATCCTGCCTGTTTAATTGCGCCGAGGTGTGTAGTTAATCCTAATGCCGCCATCGCAGTAACGAGTAACAAAGAATCCAGTTCGACTAAAAGTTTCACTAAAAAATCCgg                                    >  1:602523/1‑250 (MQ=255)                                                                                                                                                                                aataatTTACTTTTGAATTAAACAACCCACAACCAAATCACTTTTCTACAACGAGAAAAAGAGAgc                                                                                                                                                                                                                           <  1:603114/66‑1 (MQ=255)                                                                                                                                                                                  taatTTACTTTTGAATTAAACAACCCACAACCAAATCACTTTTCTACAACGAGAAAAAGAGAGCTGCTTCATAGTTCAAAAAGAATCCGCCGACGATTAACCAAATATAAATCAATGTGCCGAGAATTAACGGTTTTAATCCTGCCTGTTTAATTGCGCCGAGGTGTGTAGTTAATCCTAATGCCGCCATCGCAGTAACGa                                                                                  >  1:151545/1‑201 (MQ=255)                                                                                                                                                                                         cTTTTGAATTAAACAACCCACAACCAAATCACTTTTCTACAACGAGAAAAAGAGAGCTGCTTCATAGTTCAAAAAGAATCCGCCGACGATTAACCAAATATAAATCAATGTGCCGAGAATTAACGGTTTTAATCCTGCCTGTTTAATTGCGCCGAGGTGTGTAg                                                                                                                <  1:566639/164‑1 (MQ=255)                                                                                                                                                                                              gAATTAAACAACCCACAACCAAATCACTTTTCTACAACGAGAAAAAGAGAGCTGCTTCAt                                                                                                                                                                                                                   >  1:12729/1‑60 (MQ=255)                                                                                                                                                                                              gAATTAAACAACCCACAACCAAATCACTTTTCTACAACGAGAAAAAGAGAGCTGCTTCATAGTTCAAAAAGAATCCGCCGACGATTAACCAAatat                                                                                                                                                                               >  1:218324/1‑96 (MQ=255)                                                                                                                                                                                              gAATTAAACAACCCACAACCAAATCACTTTTCTACAACGAGAAAAAGAGAGCTGCTTCATAGTTCAAAAAGAATCCGCCGACGATTAACCAAATATAAATCAATGTGCCGAGAATTAACGGTTTTAATCCTGCCTGTTTAATTGCGCCGAGGTGTGTAGTTAATCCTAATGCCGCCATCGCAGTAACGAGTAACAAAGAATCCAGTTCGACTAAAAGTTTCACTAAAAAATCCGGCAATAAATTGAAAGaa                    >  1:293496/1‑251 (MQ=255)                                                                                                                                                                                              gAATTAAACAACCCACAACCAAATCACTTTTCTACAACGAGAAAAAGAGAGCTGCTTCATAGTTCAAAAAGAATCCGCCGACGATTAACCAAATATAAATCAATGTGCCGAGAATTAACGGTTTTAATCCTGCCTGTTTAATTGCGCCGAGGTGTGTAGTTAATCCTAATGCCGCCATCGCAGTAACGAGTAACAAAGAATCCAGTTCGACTAAAAGTTTCACTAAAAAATCCGGCAATAAATTGAAAGaa                    >  1:244236/1‑251 (MQ=255)                                                                                                                                                                                                        acCCACAACCAAATCACTTTTCTACAACGAGAAAAAGAGAGCTGCTTCATAGTTCAAAAAGAATCCGCCGACGATTAACTAAATATAAATCAATGTGCCGAGAATTAACGGTTTTAATCCTGCCTGTTTAATTGCGCCGAGGTGTGTAGTTAATCCTAATGCCGCCATCGCAGTAACGAGTAACAAAGAATCCAGTTCGACTAAAAGTTTCACTAAAAAATCCGGCAATAAATTGAAAGAATTGAATac            <  1:454232/249‑1 (MQ=255)                                                                                                                                                                                                        acCCACAACCAAATCACTTTTCTACAACGAGAAAAAGAGAGCTGCTTCATAGTTCAAAAAGAATCCGCCGACGATTAACCAAATATAAATCAATGTGCCGAGAATTAACGGTTTTAATcc                                                                                                                                             <  1:121778/120‑1 (MQ=255)                                                                                                                                                                                                        acCCACAACCAAATCACTTTTCTACAACGAGAAAAAGAGAGCTGCTTCATAGTTCAAAAAGAATCCGCCGACGATTAACCAAATATAAATCAATGTGCCGAGAATTAACGGTTTTAATCCTGCCTGTTTAATTGCGCCGAGGTGTGTAGt                                                                                                               >  1:858647/1‑150 (MQ=255)                                                                                                                                                                                                        acCCACAACCAAATCACTTTTCTACAACGAGAAAAAGAGAGCTGCTTCATAGTTCAAAAAGAATCCGCCGACGATTAACCAAATATAAATCAATGTGCCGAGAATTAACGGTTTTAATCCTGCCTGTTTAATTGCGCCGAGGTGTGTAGTTAATCCTAATGCCGCCATCGCAGTAACGAGTAACAAAGAATCCAGTTCGACTAAAAGTTTCACTAAAAAATCCGGCAATAAATTGAAAGAATTGAATaccg          >  1:361535/1‑251 (MQ=255)                                                                                                                                                                                                         cccACAACCAAATCACTTTTCTACAACGAGAAAAAGAGAGCTGCTTCATAGTTCAAAAAGAATCCGCCGACGATTAACCAAATATAAATCAATGTGCCGAGAATTAACGGTTTTAATCCTGCCTGTTTAATTGCGCCGAGGTGTGTAGTTAATCCTAATGCCGCCATCGCAGTAACGAGTAACAAAGAATCCAGTTCGACTAAAAGTTTCACTAAAAAATCCGGCAATAAATTGAAAGAATTGAATaccgc         <  1:721852/251‑1 (MQ=255)                                                                                                                                                                                                         cccACAACCAAATCACTTTTCTACAACGAGAAAAAGAGAGCTGCTTCATAGTTCAAAAAGAATCCGCCGACGATTAACCAAATATAAATCAATGTGCCGAGAATTAACGGTTTTAATCCTGCCTGTTTAATTGCGCCGAGGTGTGTAGTTAATCCTAATGCCGCCATCGCAGTAACGAGTAACAAAGAATCCAGTTCGACTAAAAGTTTCACTAAAAAATCCGGCAATAAATTGAAAGAATTGAATaccg          >  1:399673/1‑250 (MQ=255)                                                                                                                                                                                                                ccaaaTCACTTTTCTACAACGAGAAAAAGAGAGCTGCTTCATAGTTCAAAAAGAATCCGCCGACGATTAACCAAATATAAATCAATGTGCCGAGAATTAACGGTTTTAATCCTGCCTGTTTAATTGCGCCGAGGTGTGTAGTTAATCCTAATGCCGCCATCGCAGTAACGAGTAACAAAGAATCCAGTTCGACTAAAAGTTTCACTAAAAAATCCGGCAATAAATTGAAAGAATTGAAt              >  1:524906/1‑239 (MQ=255)                                                                                                                                                                                                                ccaaaTCACTTTTCTACAACGAGAAAAAGAGAGCTGCTTCATAGTTCAAAAAGAATCCGCCGACGATTAACCAAATATAAATCAATGTGCCGAGAATTAACGGTTTTAATCCTGCCTGTTTAATTGCGCCGAGGTGTGTAGTTAATCCTAATGCCGCCATCGCAGTAACGAGTAACAAAGAATCCAGTTCGACTAAAAGTTTCACTAAAAAATCCGGCAATAAATTGAAAGAATTGAATaccgcaaccgca  >  1:801775/1‑251 (MQ=255)                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                       CGCTGTTTTAACGGAATGTGAGGTAAAAAACGCCCCCCTATGCAGGTAAGCAATAAGCCGCATAGAACAAAATAAAGACTTTTACGGGATATCTGTGACATAAAATCAATTATGGATAAAAGCACTCAGAAAAGTGCGGTATGTTCCTGATGTATTTTAATAAGATATTAAAAATAATAATTTACTTTTGAATTAAACAACCCACAACCAACTCACTTTTCTACAACGAGAAAAAGAGAGCTGCTTCATAGTTCAAAAAGAATCCGCCGACGATTAACCAAATATAAATCAATGTGCCGAGAATTAACGGTTTTAATCCTGCCTGTTTAATTGCGCCGAGGTGTGTAGTTAATCCTAATGCCGCCATCGCAGTAACGAGTAACAAAGAATCCAGTTCGACTAAAAGTTTCACTAAAAAATCCGGCAATAAATTGAAAGAATTGAATACCGCAACCGCA  >  1/1573088‑1573545

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑