Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,567,498	G→T	intergenic (‑122870/‑74266)	unknown ← / → unknown	tRNA‑Met‑CAT/tRNA‑Gly‑GCC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,567,498	0	G	T	100.0%	46.4 / NA	19	intergenic (‑122870/‑74266)	unknown/unknown	tRNA‑Met‑CAT/tRNA‑Gly‑GCC
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (11/8);  total (11/8)

ATTTAACCAAGTGCCCCATTCAGGAAAAGTAGAAAGGATCCAACTTTCACGGGTGATTTCATTAACTTTAGCCATACAATAACTCCTTTTTTTAGGTTTAGTAGAATAATTTAATCATTTATAATCATAAACAATCATTTTCATTAATGGAAT‑ATTTTTTTTTAAATTTTTCTATTAAAATATGTTAAAAATTTATTAAATTCCAGACTTAGCTAACATTTTAACCTTAGATAACAACGCCAACTCTTGATTCGAAATTTCTAATCACACATAAGTATTTCAATAAAATAAATCGTGACATTGCTCACATTTTATCAAAAAACATCTTTGAAAAATCATTATAAATATTTATTATCCGCCGCCAACAAACGAAATTTACGCATGAATATTTATATTAACTCTACATTTCAAGGAGCATTACCATGGAAATCTTCATGTGGATCAACGAACAGATCCTATCTAAAGCGCCCTTACTG  >  1/1567260‑1567735                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                              aTTTAACCAAGTGCCCCATTCAGGAAAAGTAGAAAGGATCCAACTTTCACGGGTGATTTCATTAACTTTAGCCATACAATAACTCCTTTTTTTAGGTTTAGTAGAATAATTTAATCATTTATAATCATAAACAATCATTTTCATTAATGGAAT‑CTTTTTTTTTAAATTTTTCTATTAAAATATGTTAAAAATTTATTAAATTCCAGACTTAGCTAACATTTTAACCTTAGATAACAACTCCAACTCTTGAt                                                                                                                                                                                                                                   >  1:39274/1‑251 (MQ=255)                             gTAGAAAGGATCCAACTTTCACGGGTGATTTCATTAACTTTAGCCATACAATAACTCCTTTTTTTAGGTTTAGTAGAATAATTTAATCATTTATAATCATAAACAATCATTTTCATTAATGGAAT‑CTTTTTTTTTAAATTTTTCTATTAAAATATGTTAAAAATTTATTAAATTCCAGACTTAGCTAACATTTTAACCTTAGATAACAACTCCAACTCTTGATTCGAAATTTCTAATCACACATAAGTAtt                                                                                                                                                                                                       >  1:557669/1‑251 (MQ=255)                                                                           tACAATAACTCCTTTTTTTAGGTTTAGTAGAATAATTTAATCATTTATAATCATAAACAATCATTTTCATTAATGGAATCTTTTTTTTTTAAATTTTTCTATTAAAATATGTTAAAAATTTATTAAATTCCAGACTTAGCTAACATTTTAACCTTAGATAACAACTCCAACTCTTGATTCGAAATTTCTAATCACACATAAGTATTTCaa                                                                                                                                                                                                   <  1:480584/210‑1 (MQ=255)                                                                             cAATAACTCCTTTTTTTAGGTTTAGTAGAATAATTTAATCATTTATAATCATAAACAATCATTTTCATTAATGGAAT‑CTTTTTTTTTAAATTTTTCTATTAAAATATGTTAAAAATTTATTAAATTCCAGACTTAGCTAACATTTTAACCTTAGATAACAACTCCAACTCTTGATTCGAAATTTCTAATCACACATAAGTATTTCAATAAAATAAATCGTGACATTGCTCACATTTTATCAAAAAACAt                                                                                                                                                         >  1:413019/1‑249 (MQ=255)                                                                                                                                           tttCATTAATGGAAT‑CTTTTTTTTTAAATTTTTCTATTAAAATATGTTAAAAATTTATTAAATTCCAGACTTAGCTAACATTTTAACCTTAGATAACAACTCCAACTCTTGATTCGAAATTTCTAATCACACATAAGTATTTCAATAAAATAAATCGTGACATTGCTCACATTTTATCAAAAAACATCTTTGAAAAATCATTATAAATATTTATTATCCGCCGCCAACAAACGAAATTTACGCATGAatat                                                                                         <  1:795975/251‑1 (MQ=255)                                                                                                                                                        aatctttttttttAAATTTTTCTATTAAAATATGTTAAAAATTTATTAAATTCCAGACTTAGCTAACATTTTAACCTTAGATAACAACTCCAACTCTTGATTCGAAATTTCTAATCACACATAAGTATTTCAATAAAATAAATCGTGACATTGCTCACATTTTATCAAAAAACATCTTTGAAAAATCATTATAAATATTTATTATCCGCCGCCAACAAACGAAATTTACGCATGAATATTTATATTAActc                                                                             <  1:297921/247‑1 (MQ=255)                                                                                                                                                           ctttttttttAAATTTTTCTATTAAAATATGTTAAAAATTTATTAAATTCCAGACTTAGCTAACATTTTAACCTTAGATAACAACTCCAACTCTTGATTCGAAATTTCTAATCACACATAAGTATTTCAATAAAATAAATCGTGACATTGCTCACATTTTATCAAAAAACATCTTTGAAAAATCATTATAAATATTTATTATCCGCCGCCAACAAACGAAATTTACGCATGAATATTTATATTAACTCTAc                                                                          <  1:181147/250‑1 (MQ=255)                                                                                                                                                                                    aaTATGTTAAAAATTTATTAAATTCCAGACTTAGCTAACATTTTAACCTTAGATAACAACTCCAACTCTTGATTCGAAATTTCTAATCACACATAAGTATTTCAATAAAATAAATCGTGACATTGCTCACATTTTATCAAAAAACATCTTTGAAAAATCATTATAAATATTTATTATCCGCCGCCAACAAACGAAATTTACGCATGAATATTTATATTAACTCTACATTTCAAGGAGCATTACCATGGaaa                                                 <  1:852044/251‑1 (MQ=255)                                                                                                                                                                                                         aTTCCAGACTTAGCTAACATTTTAACCTTAGATAACAACTCCAACTCTTGATTCGAAATTTCTAATCACACATAAGTATTTCAATAAAATAAATCGTGACATTGCTCACATTTTATCAAAAAACATCTTTGAAAAATCATTATAAATATTTATTATCCGCCGCCAACAAACGAAATTTACGCATGAATATTTATATTAACTCTACATTTCAAGGAGCATTACCATGGAAATCTTCATGTGGATCAACGaa                             >  1:413690/1‑250 (MQ=255)                                                                                                                                                                                                         aTTCCAGACTTAGCTAACATTTTAACCTTAGATAACAACTCCAACTCTTGATTCGAAATTTCTAATCACACATAAGTATTTCAATAAAATAAATCGTGACATTGCTCACATTTTATCAAAAAACATCTTTGAAAAATCATTATAAATATTTATTATCCGCCGCCAACAAACGAAATTTACGCATGAATATTTATATTAACTCTACATTTCAAGGAGCATTACCATGGAAATCTTCATGTGGATCAACGaa                             >  1:326299/1‑250 (MQ=255)                                                                                                                                                                                                            ccAGACTTAGCTAACATTTTAACCTTAGATAACAACTCCAACTCTTGATTCGAAATTTCTAATCACACATAAGTATTTCAATAAAATAAATCGTGACATTGCTCACATTTTATCAAAAAACATCTTTGAAAAATCATTATAAATATTTATTATCCGCCGCCAACAAACGCAATTTACGCATGAATATTTATATTAACTCTACATTTCAAGGAGCATTACCATGGAAATCTTCATGTGGATCAACGAACAGa                         >  1:447939/1‑251 (MQ=255)                                                                                                                                                                                                               gACTTAGCTAACATTTTAACCTTAGATAACAACTCCAACTCTTGATTCGAAATTTCTAATCACACATAAGTAt                                                                                                                                                                                                        <  1:388145/73‑1 (MQ=255)                                                                                                                                                                                                               gACTTAGCTAACATTTTAACCTTAGATAACAACTCCAACTCTTGATTCGAAATTTCTAATCACACATAAGTATTTCAATAAAATAAATCGTGACATTGCTCACATTTTATCAAAAAACATCTTTGAAAAATCATTATAAATATTTATTATCCGCCGCCAACAAACGAAATTTACGCATGAATATTTATATTAACTCTACATTTCAAGGAGCATTACCATGGAAATCTTCATGTGGATCAACGAACAGATc                       >  1:406087/1‑250 (MQ=255)                                                                                                                                                                                                                    aGCTAACATTTTAACCTTAGATAACAACTCCAACTCTTGATTCGAAATTTCTAATCACACATAAGTAttt                                                                                                                                                                                                      >  1:801390/1‑70 (MQ=255)                                                                                                                                                                                                                          cATTTTAACCTTAGATAACAACTCCAACTCTTGATTCGAAATTTCTAATCACACATAAGTATTTCAATAAAATAAATCGTGACATTGCTCACATTTTATCAAAAAACATCTTTGAAAAATCATTATAAATATTTATTATCCGCCGCCAACAAACGAAATTTACGCATGAATATTTATATTa                                                                                 <  1:121415/181‑1 (MQ=255)                                                                                                                                                                                                                          cATTTTAACCTTAGATAACAACTCCAACTCTTGATTCGAAATTTCTAATCACACATAAGTATTTCAATAAAATAAATCGTGACATTGCTCACATTTTATCAAAAAACATCTTTGAAAAATCATTATAAATATTTATTATCCGCCGCCAACAAACGAAATTTACGCATGAATATTTATATTAACTCTACATTTCAAGGAGCATTAc                                                         >  1:67425/1‑205 (MQ=255)                                                                                                                                                                                                                           aTTTTAACCTTAGATAACAACTCCAACTCTTGATTCGAAATTTCTAATCACACATAAGTATTTCAATAAAATAAATCGTGACATTGCTCACATTTTATCAAAAAACATCTTTGAAAAATCATTATAAATATTTATTATCCGCCGCCAACAAACGAAATTTACGCATGAATATTTATATTAACTCTACATTTc                                                                     <  1:809006/192‑1 (MQ=255)                                                                                                                                                                                                                            ttttAACCTTAGATAACAACTCCAACTCTTGATTCGAAATTTCTAATCACACATAAGTATTTCAATAAAATAAATCGTGACATTGCTCACATTTTATCAAAAAACATCTTTGAAAAATCATTATAAATATTTATTATCCGCCGCCAACAAACGAAATTTACGCATGAATATTTATATTAACTCTACATTTCAAGGAGCATTACCATGGAAATCTTCATGTGGAt                                    >  1:292780/1‑224 (MQ=255)                                                                                                                                                                                                                                    ttAGATAACAACTCCAACTCTTGATTCGAAATTTCTAATCACACATAAGTATTTCAATAAAATAAATCGTGACATTGCTCACATTTTATCAAAAAACATCTTTGAAAAATCATTATAAATATTTATTATCCGCCGCCAACAAACGAAATTTACGCATGAATATTTATATTAACTCTACATTTCAAGGAGCATTACCATGGAAATCTTCATGTGGATCAACGAACAGATCCTATCTAAAGCGCCCTTACTg  >  1:712279/1‑250 (MQ=255)                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                              ATTTAACCAAGTGCCCCATTCAGGAAAAGTAGAAAGGATCCAACTTTCACGGGTGATTTCATTAACTTTAGCCATACAATAACTCCTTTTTTTAGGTTTAGTAGAATAATTTAATCATTTATAATCATAAACAATCATTTTCATTAATGGAAT‑ATTTTTTTTTAAATTTTTCTATTAAAATATGTTAAAAATTTATTAAATTCCAGACTTAGCTAACATTTTAACCTTAGATAACAACGCCAACTCTTGATTCGAAATTTCTAATCACACATAAGTATTTCAATAAAATAAATCGTGACATTGCTCACATTTTATCAAAAAACATCTTTGAAAAATCATTATAAATATTTATTATCCGCCGCCAACAAACGAAATTTACGCATGAATATTTATATTAACTCTACATTTCAAGGAGCATTACCATGGAAATCTTCATGTGGATCAACGAACAGATCCTATCTAAAGCGCCCTTACTG  >  1/1567260‑1567735

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑