Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,562,315	T→G	intergenic (‑117687/‑79449)	unknown ← / → unknown	tRNA‑Met‑CAT/tRNA‑Gly‑GCC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,562,315	0	T	G	100.0%	180.7 / NA	64	intergenic (‑117687/‑79449)	unknown/unknown	tRNA‑Met‑CAT/tRNA‑Gly‑GCC
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (33/31);  total (33/31)

ATCCTGTTCCTCCAAATAACTGCCGTCACACCATTTCAGCACGATAATATCGAGGATATTCTCGTCTAACTGTAAATAATTCGTTACATCAAATTCAGAGGTGCAATGGCTGATTTGGCTATAACCCACGAATTGCCCGTTCAACACCATAAAATAATCGTAAAATTCTCTTGGATTTTCCACCGCACTTTGGTCATTGGCATGGGGAATAAAATAGGCGTGTTGCGGTGTTGATGTGTAATGTTTCTAAATCCTGAAAGTAATTGAGTAATGTCATGGCGGAACCCCTTACAGCGGAATGAAAATTTTTACTATACTCAATCCGCCCCATAAATTTCACCTAAAATTAAGATTTTGCGGCTTATATCACGATCTTTGTTTTACAGAATGTAAAATTTAAAATACAATCTCCGTCAACTTTTAAATACACAATAAGGAAAATCTATGAACAAAACCGTAGGAAGTACGCTGTTAGTGGCAGGAACCATGA  >  1/1562071‑1562560                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                      aTCCTGTTCCTCCAAATAACTGCCGTCACACCATTTCAGCACGATAATATCGAGGATATTCTCGTCTAACTGTAAATAATTCGTTACATCAAATTCAGAGGTGCAATGGCTGATTTGGCTATAACCCACGAATTGCCCGTTCAACACCATAAAATAATCGTAAAATTCTCTTGGATTTTCCACCGCACTTTGGTCATTGGCATGGGGAATAAAATAGGCGTGTTGCGGTGTTGATGTGTAATGTGTCTaaa                                                                                                                                                                                                                                                 <  1:276663/251‑1 (MQ=255)           tccAAATAACTGCCGTCACACCATTTCAGCACGATAATATCGAGGATATTCTCGTCTAACTGTAAATAATTCGTTACATCAAATTCAGAGGTGCAATGGCTGATTTGGCTATAACCCACGAATTGCCCGTTCAACACCATAAAATAATCGTAAAATTCTCTTGGATTTTCCACCGCACTTTGGTCATTGGCATGGGGAATAAAATAGGCGTGTTGCGGTGTTGATGTGTAATGTGTCTAAATCCTGAAAGt                                                                                                                                                                                                                                       <  1:793711/251‑1 (MQ=255)            ccAAATAACTGCCGTCACACCATTTCAGCACGATAATATCGAGGATATTCTCGTCTAACTGTAAATAATTCGTTACATCAAATTCAGAGGTGCAATGGCTGATTTGGCTATAACCCACGAATTGCCCGTTCAACACCATAAAATAATCGTAAAATTCTCTTGGATTTTCCACCGCACTTTGGTCATTGGCATGGGGAATAAAATAGGCGTGTTGCGGTGTTGATGTGTAATGTGTCTAAATc                                                                                                                                                                                                                                               >  1:109971/1‑242 (MQ=255)                        cGTCACACCATTTCAGCACGATAATATCGAGGATATTCTCGTCTAACTGTAAATAATTCGTTACATCAAATTCAGAGGTGCAATGGCTGATTTGGCTATAACCCACGAATTGCCCGTTCAACACCATAAAATAATCGTAAAATTCTCTTGGATTTTCCACCGCACTTTGGTCATTGGCATGGGGAATAAAATAGGCGTGTTGCGGTGTTGATGTGTAATGTGTCTAAATCCTGAAAGTAATTGAGTAATGt                                                                                                                                                                                                                          <  1:107017/251‑1 (MQ=255)                        cGTCACACCATTTCAGCACGATAATATCGAGGATATTCTCGTCTAACTGTAAATAATTCGTTACATCAAATTCAGAGGTGCAATGGCTGATTTGGCTATAACCCACGAATTGCCCGTTCAACACCATAAAATAATCGTAAAATTCTCTTGGATTTTCCACCGCACTTTGGTCATTGGCATGGGGAATAAAATAGGCGTGTTGCGGTGTTGATGTGTAATGTGTCTAAATCCTGAAAGTAATTGAGTAATGt                                                                                                                                                                                                                          <  1:282327/251‑1 (MQ=255)                             cacCATTTCAGCACGATAATATCGAGGATATTCTCGTCTAACTGTAAATAATTCGTTACATCAAATTCAGAGGTGCAATGGCTGATTTGGCTATAACCCACGAATTGCCCGTTCAACACCATAAAATAATCGTAAAATTCTCTTGGATTTTCCACCGCACTTTGGTCATTGGCATGGGGAATAAAATAGGCGTGTTGCGGTGTTGATGTGTAATGTGTCTaaa                                                                                                                                                                                                                                                 >  1:779517/1‑223 (MQ=255)                             cacCATTTCAGCACGATAATATCGAGGATATTCTCGTCTAACTGTAAATAATTCGTTACATCAAATTCAGAGGTGCAATGGCTGATTTGGCTATAACCCACGAATTGCCCGTTCAACACCATAAAATAATCGTAAAATTCTCTTGGATTTTCCACCGCACTTTGGTCATTGGCATGGGGAATAAAATAGGCGTGTTGCGGTGTTGATGTGTAATGTGTCTAAATCCTGAAAGTAATTGAGTAATGTCATgg                                                                                                                                                                                                                     >  1:525135/1‑251 (MQ=255)                               ccATTTCAGCACGATAATATCGAGGATATTCTCGTCTAACTGTAAATAATTCGTTACATCAAATTCAGAGGTGCAATGGCTGATTTGGCCATAACCCACGAATTGCCCGTTCAACACCATAAAATAATCGTAAAATTCTCTTGGATTTTCCACCGCACTTTGGTCATTGGCATGGGGAATAAAATAGGCGTGTTGCGGTGTTGATGTGTAATGTGTCTAAATCCTGAAAGTAATTGAGTAATGTCATGGCg                                                                                                                                                                                                                   >  1:391456/1‑251 (MQ=255)                                cATTTCAGCACGATAATATCGAGGATATTCTCGTCTAACTGTAAATAATTCGTTACATCAAATTCAGAGGTGCAATGGCTGATTTGGCTATAACCCACGAATTGCCCGTTCAACACCATAAAATAATCGTAAAATTCTCTTGGATTTTCCACCGCACTTTGGTCATTGGCATGGGGAATAAAATAGGCGTGTTGCGGTGTTGATGTGTAATGTGTCTAAATCCTGAAAGTAATTGAGTAATGTCATGGCgg                                                                                                                                                                                                                  <  1:335385/251‑1 (MQ=255)                                  tttCAGCACGATAATATCGAGGATATTCTCGTCTAACTGTAAATAATTCGTTACATCAAATTCAGAGGTGCAATGGCTGATTTGGCTATAACCCACGAATTGCCCGTTCAACACCATAAAATAATCGTAAAATTCTCTTGGATTTTCCACCGCACTTTGGTCATTGGCATGGGGAATAAAATAGGCGTGTTGCGGTGTTGATGTGTAATGTGTCTAAATCCTGAAAGTAATTGAGTAATGTCATGGCGGaa                                                                                                                                                                                                                >  1:207383/1‑251 (MQ=255)                                          cGATAATATCGAGGATATTCTCGTCTAACTGTAAATAATTCGTTACATCAAATTCAGAGGTGCAATGGCTGATTTGGCTATAACCCACGAATTGCCCGTTCAACACCATAAAATAATCGTAAAATTCTCTTGGATTTTCCACCGCACTTTGGTCATTGGCATGGGGAATAAAATAGGCGTGTTGCGGTGTTGATGTGTAATGTGTCTAAATCCTGAAAGTAATTGAGTAATGTCATGGCGGAACCCCTTa                                                                                                                                                                                                         >  1:217009/1‑250 (MQ=255)                                          cGATAATATCGAGGATATTCTCGTCTAACTGTAAATAATTCGTTACATCAAATTCAGAGGTGCAATGGCTGATTTGGCTATAACCCACGAATTGCCCGTTCAACACCATAAAATAATCGTAAAATTCTCTTGGATTTTCCACCGCACTTTGGTCATTGGCATGGGGAATAAAATAGGCGTGTTGCGGTGTTGATGTGTAATGTGTCTAAATCCTGAAAGTAATTGAGTAATGTCATGGCGGAACCCCTTAc                                                                                                                                                                                                        <  1:447674/251‑1 (MQ=255)                                            ataataTCGAGGATATTCTCGTCTAACTGTAAATAATTCGTTACATCAAATTCAGAGGTGCAATGGCTGATTTGGCTATAACCCACGAATTGCCCGTTCAACACCATAAAATAATCGTAAAATTCTCTTGGATTTTCCACCGCACTTTGGTCATTGGCATGGGGAATAAAATAGGCGTGTTGCGGTGTTGATGTGTAATGTGTCTAAATCCTGAAAg                                                                                                                                                                                                                                        >  1:65424/1‑217 (MQ=255)                                            ataataTCGAGGATATTCTCGTCTAACTGTAAATAATTCGTTACATCAAATTCAGAGGTGCAATGGCTGATTTGGCTATAACCCACGAATTGCCCGTTCAACACCATAAAATAATCGTAAAATTCTCTTGGATTTTCCACCGCACTTTGGTCATTGGCATGGGGAATAAAATAGGCGTGTTGCGGTGTTGATGTGTAATGTGTCTAAATCCTGAAAGTAAt                                                                                                                                                                                                                                    >  1:607265/1‑221 (MQ=255)                                               atatCGAGGATATTCTCGTCTAACTGTAAATAATTCGTTACATCAAATTCAGAGGTGCAATGGCTGATTTGGCTATAACCCACGAATTGCCCGTTCAACACCATAAAATAATCGTAAAATTCTCTTGGATTTTCCACCGCACTTTGGTCATTGGCATGGGGAATAAAATAGGCGTGTTGCGGTGTTGATGTGTAATGTGTCTAAATCCTGAAAGTAATTGAGTAATGTCATGGCGGAACCCCTTACAGCgg                                                                                                                                                                                                   >  1:788106/1‑251 (MQ=255)                                                 atCGAGGATATTCTCGTCTAACTGTAAATAATTCGTTACATCAAATTCAGAGGTGCAATGGCTGATTTGGCTATAACCCACGAATTGCCCGTTCAACACCATAAAATAATCGTAAAATTCTCTTGGATTTTCCACCGCACTTTGGTCATTGGCATGGGGAATAAAATAGGCGTGTTGCGGTGTTGATGTGTAATGTGTCTAAATCCTGAAAGTAATTGAGTAATGTCATGGCGGAACCCCTTACAGCGGaa                                                                                                                                                                                                 >  1:170095/1‑251 (MQ=255)                                                 atCGAGGATATTCTCGTCTAACTGTAAATAATTCGTTACACCAAATTCAGAGGTGCAATGGCTGATTTGGCTATAACCCACGAATTGCCCGTTCAACACCATAAAATAATCGTAAAATTCTCTTGGATTTTCCACCGCACTTTGGTCATTGGCATGGGGAATAAAATAGGCGTGTTGCGGTGTTGATGTGTAATGTGTCTAAATCCTGAAAGTAATTGAGTAATGTCATGGCGGAACCCCTTACAGCGGaa                                                                                                                                                                                                 >  1:217564/1‑251 (MQ=255)                                                      ggATATTCTCGTCTAACTGTAAATAATTCGTTACATCAAATTCAGAGGTGCAATGGCTGATTTGGCTATAACCCACGAATTGCCCGTTCAACACCATAAAATAATCGTAAAATTCTCTTGGATTTTCCACCGCACTTTGGTCATTGGCATGGGGAATAAAATAGGCGTGTTGCGGTGTTGATGTGTAATGTGTCTAAATCCTGAAAGTAATTGAGTAATGTCATGGCg                                                                                                                                                                                                                   <  1:653276/228‑1 (MQ=255)                                                                     aCTGTAAATAATTCGTTACATCAAATTCAGAGGTGCAATGGCTGATTTGGCTATAACCCACGAATTGCCCGTTCAACACCATAAAATAATCGTAAAATTCTCTTGGATTTTCCACCGCACTTTGGTCATTGGCATGGGGAATAAAATAGGCGTGTTGCGGTGTTGATGTGTAATGTGTCTAAATCCTGAAAGTAATTGAGTAATGTCATGGCGGAACCCCTTACAGCGGAATGAAAATTTTTACTATACt                                                                                                                                                                              >  1:197464/1‑250 (MQ=255)                                                                        gTAAATAATTCGTTACATCAAATTCAGAGGTGCAATGGCTGATTTGGCTATAACCCACGAATTGCCCGTTCAACACCATAAAATAATCGTAAAATTCTCTTGGATTTTCCACCGCACTTTGGTCATTGGCATGGGGAATAAAATAGGCGTGTTGCGGTGTTGATGTGTAATGTGTCTAAATCCTGAAAGTAATTGAGTAATGt                                                                                                                                                                                                                          >  1:756316/1‑203 (MQ=255)                                                                         tAAATAATTCGTTACATCAAATTCAGAGGTGCAATGGCTGATTTGGCTATAACCCACGAATTGCCCGTTCAACACCATAAAATAATCGTAAAATTCTCTTGGATTTTCCACCGCACTTTGGTCATTGGCATGGGGAATAAAATAGGCGTGTTGCGGTGTTGATGTGTAATGTGTCTAAATCCTGAAAGTAATTGAGTAATGTCATGGCGGAACCCCTTACAGCGGAATGAAAATTTTTACTATACTCAAt                                                                                                                                                                          <  1:362108/250‑1 (MQ=255)                                                                                      aCATCAAATTCAGAGGTGCAATGGCTGATTTGGCTATAACCCACGAATTGCCCGTTCAACACCATAAAATAATCGTAAAATTCTCTTGGATTTTCCACCGCACTTTGGTCATTGGCATGGGGAATAAAATAGGCGTGTTGCGGTGTTGATGTGTAATGTGTCTAAATCCTGAAAGTAATTGAGTAATGTCATGGCGGAACCCCTTACAGCGGAATGAAAATTTTTACTGTACTCAATCCGCCCCATAAAtt                                                                                                                                                            <  1:673602/251‑1 (MQ=255)                                                                                      aCATCAAATTCAGAGGTGCAATGGCTGATTTGGCTATAACCCACGAATTGCCCGTTCAACACCATAAAATAATCGTAAAATTCTCTTGGATTTTCCACCGCACTTTGGTCATTGGCATGGGGAATAAAATAGGCGTGTTGCGGTGTTGATGTGTAATGTGTCTAAATCCTGAAAGTAATTGAGTAATGTCATGGCGGAACCCCTTACAGCGGAATGAAAATTTTTACTATACTCAATCCGCCCCATAAAtt                                                                                                                                                            <  1:408724/251‑1 (MQ=255)                                                                                                     gTGCAATGGCTGATTTGGCTATAACCCACGAATTGCCCGTTCAACACCATAAAATAATCGTAAAATTCTCTTGGATTTTCCACCGCACTTTGGTCATTGGCATGGGGAATAAAATAGGCGTGTTGCGGTGTTGATGTGTAATGTGTCTAAATCCTGAAAGTAATTGAGTAATGTCATGGCGGAACCCCTTACAGCGGAATGAAAATTTTTACTATACTCAATCCGCCCCATAAATTTCACCTAAAATTAAg                                                                                                                                             >  1:641947/1‑251 (MQ=255)                                                                                                        cAATGGCTGATTTGGCTATAACCCACGAATTGCCCGTTCAACACCATAAAATAATCGTAAAATTCTCTTGGATTTTCCACCGCACTTTGGTCATTGGCATGGGGAATAAAATAGGCGTGTTGCGGTGTTGATGTGTAATGTGTCTAAATCCTGAAAGTAATTGAGTAATGTCATGGCGGAACCCCTTACAGCGGAATGAAAATTTTTACTATACTCAATCCGCCCCATAAATTTCACCTAAAATTAAGAt                                                                                                                                           <  1:118841/250‑1 (MQ=255)                                                                                                                  tttGGCTATAACCCACGAATTGCCCGTTCAACACCATAAAATAATCGTAAAATTCTCTTGGATTTTCCACCGCACTTTGGTCATTGGCATGGGGAATAAAATAGGCGTGTTGCGGTGTTGATGTGTAATGTGTCTAAATCCTGAAAGTAATTGAGTAATGTCATGGCGGAACCCCTTACAGCGGAATGAAAATTTTTACTATACTCAATCCGCCCCATAAATTTCACCTAAAATTAAGATTTTGCGGCTta                                                                                                                                >  1:703198/1‑251 (MQ=255)                                                                                                                      gCTATAACCCACGAATTGCCCGTTCAACACCATAAAATAATCGTAAAATTCTCTTGGATTTTCCACCGCACTTTGGTCATTGGCATGGGGAATAAAATAGGCGTGTTGCGGTGTTGATGTGTAATGTGTCTAAATCCTGAAAGTAATTGAGTAATGTCATGGCGGAACCCCTTACAGCGGAATGAAAATTTTTACTATACTCAATCCGCCCCATAAATTTCACCTAAAATTAAGATTTTGCGGCTTatat                                                                                                                             >  1:361497/1‑250 (MQ=255)                                                                                                                      gCTATAACCCACGAATTGCCCGTTCAACACCATAAAATAATCGTAAAATTCTCTTGGATTTTCCACCGCACTTTGGTCATTGGCATGGGGAATAAAATAGGCGTGTTGCGGTGTTGATGTGTAATGTGTCTAAATCCTGAAAGTAATTGAGTAATGTCATGGCGGAACCCCTTACAGCGGAATGAAAATTTTTACTATACTCAATCCGCCCCATAAATTTCACCTAAAATTAAGATTTTGCGGCTTatat                                                                                                                             >  1:670180/1‑250 (MQ=255)                                                                                                                       cTATAACCCACGAATTGCCCGTTCAACACCATAAAATAATCGTAAAATTCTCTTGGATTTTCCACCGCACTTTGGTCATTGGCATGGGGAATAAAATAGGCGTGTTGCGGTGTTGATGTGTAATGTGTCTAAATCCTGAAAGTAATTGAGTAATGTCATGGCGGAACCCCTTACAGCGGAATGAAAATTTTTACTATACTCAATCCGCCCCATAAATTTCACCTAAAATTAAGATTTTGCGGCTTATATc                                                                                                                            <  1:49941/250‑1 (MQ=255)                                                                                                                                      tGCCCGTTCAACACCATAAAATAATCGTAAAATTCTCTTGGATTTTCCACCGCACTTTGGTCATTGGCATGGGGAATAAAATAGGCGTGTTGCGGTGTTGATGTGTAATGTGTCTAAATCCTGAAAGTAATTGAGTAATGTCATGGCGGAACCCCTTACAGCGGAATGAAAATTTTTACTATACTCAATCCGCCCCATAAATTTCACCTAAAATTAAGATTTTGCGGCTTATATCACGATCTTTGTTTTAc                                                                                                            <  1:666288/251‑1 (MQ=255)                                                                                                                                           gTTCAACACCATAAAATAATCGTAAAATTCTCTTGGATTTTCCACCGCACTTTGGTCATTGGCATGGGGAATAAAATAGGCGTGTTGCGGTGTTGATGTGTAATGTGTCTAAATCCTGAAAGTAATTg                                                                                                                                                                                                                                  <  1:769735/128‑1 (MQ=255)                                                                                                                                           gTTCAACACCATAAAATAATCGTAAAATTCTCTTGGATTTTCCACCGCACTTTGGTCATTGGCATGGGGAATAAAATAGGCGTGTTGCGGTGTTGATGTGTAATGTGTCTAAATCCTGAAAGTAATTGAg                                                                                                                                                                                                                                >  1:71337/1‑130 (MQ=255)                                                                                                                                           gTTCAACACCATAAAATAATCGTAAAATTCTCTTGGATTTTCCACCGCACTTTGGTCATTGGCATGGGGAATAAAATAGGCGTGTTGCGGTGTTGATGTGTAATGTGTCTAAATCCTGAAAGTAATTGAGTAATGTCATGGCGGAACCCCTTACAGCGGAATGAAAATTTTTACTATACTCAATCCGCCCCATAAATTTCACCTAAAATTAAGATTTTGCGGCTTATATCACGATCTTTGTTTTACAg                                                                                                          <  1:110351/248‑1 (MQ=255)                                                                                                                                           gTTCAACACCATAAAATAATCGTAAAATTCTCTTGGATTTTCCACCGCACTTTGGTCATTGGCATGGGGAATAAAATAGGCGTGTTGCGGTGTTGATGTGTAATGTGTCTAAATCCTGAAAGTAATTGAGTAATGTCATGGCGGAACCCCTTACAGCGGAATGAAAATTTTTACTATACTCAATCCGCCCCATAAATTTCACCTAAAATTAAGATTTTGCGGCTTATATCACGATCTTTGTTTTACAGAAt                                                                                                       >  1:531517/1‑251 (MQ=255)                                                                                                                                                   ccATAAAATAATCGTAAAATTCTCTTGGATTTTCCACCGCACTTTGGTCATTGGCATGGGGAATAAAATAGGCGTGTTGCGGTGTTGATGTGTAATGTGTCTAAATCCTGAAAGTAATTGAGTAATGTCATGGCGGAACCCCTTACAGCGGAATGAAAATTTTTACTATGCTCAATCCGCCCCATAAATTTCACCTAAAATTAAGATTTTGCGGCTTATATc                                                                                                                            >  1:455781/1‑222 (MQ=255)                                                                                                                                                   ccATAAAATAATCGTAAAATTCTCTTGGATTTTCCACCGCACTTTGGTCATTGGCATGGGGAATAAAATAGGCGTGTTGCGGTGTTGATGTGTAATGTGTCTAAATCCTGAAAGTAATTGAGTAATGTCATGGCGGAACCCCTTACAGCGGAATGAAAATTTTTACTATACTCAAt                                                                                                                                                                          >  1:568265/1‑176 (MQ=255)                                                                                                                                                   ccATAAAATAATCGTAAAATTCTCTTGGATTTTCCACCGCACTTTGGTCATTGGCATGGGGAATAAAATAGGCGTGTTGCGGTGTTGATGTGTAATGTGTCTAAATCCTGAAAGTAATTGAGTAATGTCATGGCGGAACCCCTTACAGCGGAATGAAAATTTTTACTATACTCAATCCGCCCCATAAATTTCACCTAAAATTAAGATTTTGCGGCTTATATCACGa                                                                                                                        >  1:570691/1‑226 (MQ=255)                                                                                                                                                   ccATAAAATAATCGTAAAATTCTCTTGGATTTTCCACCGCACTTTGGTCATTGGCATGGGGAATAAAATAGGCGTGTTGCGGTGTTGATGTGTAATGTGTCTAAATCCTGAAAGTAATTGAGTAATGTCATGGCGGAACCCCTTACAGCGGAATGAAAATTTTTACTATACTCAATCCGCCCCATAAATTTCACCTAAAATTAAGATTTTGCGGCTTATATCACGATCTTTGTTTTACAGAATGTAAAAt                                                                                                >  1:463805/1‑250 (MQ=255)                                                                                                                                                              tCGTAAAATTCTCTTGGATTTTCCACCGCACTTTGGTCATTGGCATGGGGAATAAAATAGGCGTGTTGCGGTGTTGATGTGTAATGTGTCTAAATCCTGAAAGTAATTGAGTAATGTCATGGCGGAACCCCTTACAGCGGAATGAAAATTTTTACTATACTCAATCCGCCCCATAAATTTCACCTAAAATTAAGATTTTGCGGCTTATATCACGATCTTTGTTTTACAg                                                                                                          >  1:503062/1‑229 (MQ=255)                                                                                                                                                                        ctctTGGATTTTCCACCGCACTTTGGTCATTGGCATGGGGAATAAAATAGGCGTGTTGCGGTGTTGATGTGTAATGTGTCTAAATCCTGAAAGTAATTGAGTAATGTCATGGCGGAACCCCTTACAGCGGAATGAAAATTTTTACTATACTCAATCCGCCCCATAAATTTCACCTAAAATTAAGATTTTGCGGCTTATATCACGATCTTTGTTTTACAGAATGTAAAAt                                                                                                <  1:820828/229‑1 (MQ=255)                                                                                                                                                                           ttGGATTTTCCACCGCACTTTGGTCATTGGCATGGGGAATAAAATAGGCGTGTTGCGGTGTTGATGTGTAATGTGTCTAAATCCTGAAAGTAATTGAGTAATGTCATGGCGGAACCCCTTACAGCGGAATGAAAATTTTTACTATACTCAATCCGCCCCATAAATTTCACCTAAAATTAAGATTTTGCGGCTTATATCACGATCTTTGTTTTACAGAATGTAAAATTTAAAATACAATCTCCGTCAACttt                                                                       <  1:894625/251‑1 (MQ=255)                                                                                                                                                                                          cACTTTGGTCATTGGCATGGGGAATAAAATAGGCGTGTTGCGGTGTTGATGTGTAATGTGTCTAAATCCTGAAAGTAATTGAGTAATGTCATGGCGGAACCCCTTACAGCGGAATGAAAATTTTTACTATACTCAATCCGCCCCATAAATTTCACCTAAAATTAAGATTTTGCGGCTTATATCACGATCTTTGTTTTACAGAATGTAAAATTTAAAATACAATCTCCGTCAACTTTTAAATACACAATAAg                                                        <  1:900802/251‑1 (MQ=255)                                                                                                                                                                                            cTTTGGTCATTGGCATGGGGAATAAAATAGGCGTGTTGCGGTGTTGATGTGTAATGTGTCTAAATCCTGAAAGTAATTGAGTAATGTCATGGCGGAACCCCTTACAGCGGAATGAAAATTTTTACTATACTCAATCCGCCCCATAAATTTCACCTAAAATTAAGATTTTGCGGCTTATATCACGATCTTTGTTTTACAGAATGTAAAATTTAAAATACAATCTCCGTCAACTTTTAAATACACAATAAGGa                                                      <  1:554422/251‑1 (MQ=255)                                                                                                                                                                                                 gTCATTGGCATGGGGAATAAAATAGGCGTGTTGCGGTGTTGATGTGTAATGTGTCTAAATCCTGAAAg                                                                                                                                                                                                                                        <  1:345183/68‑1 (MQ=255)                                                                                                                                                                                                               gAATAAAATAGGCGTGTTGCGGTGTTGATGTGTAATGTGTCTAAATCCTGAAAGTAATTGAGTAATGTCATGGCGGAACCCCTTACAGCGGAATGAAAATTTTTACTATACTc                                                                                                                                                                             <  1:43344/113‑1 (MQ=255)                                                                                                                                                                                                               gAATAAAATAGGCGTGTTGCGGTGTTGATGTGTAATGTGTCTAAATCCTGAAAGTAATTGAGTAATGTCATGGCGGAACCCCTTACAGCGGAATGAAAATTTTTACTATACTCAATCCGCCCCATAAATTTCACCTAAAATTAAGATTTTGCGGCTTATATCACGATCTTTGtt                                                                                                                <  1:147267/174‑1 (MQ=255)                                                                                                                                                                                                               gAATAAAATAGGCGTGTTGCGGTGTTGATGTGTAATGTGTCTAAATCCTGAAAGTAATTGAGTAATGTCATGGCGGAACCCCTTACAGCGGAATGAAAATTTTTACTATACTCAATCCGCCCCATAAATTTCACCTAAAATTAAGATTTTGCGGCTTATATCACGATCTTTGTTTTAc                                                                                                            >  1:886375/1‑178 (MQ=255)                                                                                                                                                                                                               gAATAAAATAGGCGTGTTGCGGTGTTGATGTGTAATGTGTCTAAATCCTGAAAGTAATTGAGTAATGTCATGGCGGAACCCCTTACAGCGGAATGAAAATTTTTACTATACTCAATCCGCCCCATAAATTTCACCTAAAATTAAGATTTTGCGGCTTATATCACGATCTTTGTTTTACAGAATGTAAAATTTAAAATACAAtc                                                                                   >  1:65055/1‑203 (MQ=255)                                                                                                                                                                                                                   aaaaTAGGCGTGTTGCGGTGTTGATGTGTAATGTGTCTAAATCCTGAAAGTAATTGAGTAATGTCATGGCGGAACCCCTTACAGCGGAATGAAAATTTTTACTATACTCAATCCGCCCCATAAATTTCACCTAAAATTAAGATTTTGCGGCTTATATCACGATCTTTGTTTTACAGAATGTAAAATTTAAAATACAATCTCCGTCAACTTTTAAATACACAATAAGGAAAATCTATGAACAAAACCGTAgg                               <  1:97013/251‑1 (MQ=255)                                                                                                                                                                                                                            gtgtTGCGGTGTTGATGTGTAATGTGTCTAAATCCTGAAAGTAATTGAGTAATGTCATGGCGGAACCCCTTACAGCGGAATGAAAATTTTTACTATACTCAATCCGCCCCATAAATTTCACCTAAAATTAAGATTTTGCGGCTTATATCACGATCTTTGTTTTACAGAATGTAAAATTTAAAATACAATCTCCGTCAACTTTTAAATACACAATAAGGAAAATCTATGAACAAAACCGTAGGAAGTACg                        >  1:625446/1‑249 (MQ=255)                                                                                                                                                                                                                              gtTGCGGTGTTGATGTGTAATGTGTCTAAATCCTGAAAGTAATTGAGTAATGt                                                                                                                                                                                                                          <  1:218292/53‑1 (MQ=255)                                                                                                                                                                                                                                 gCGGTGTTGATGTGTAATGTGTCTAAATCCTGAAAGTAATTGAGTAATGTCATGGCGGAACCCCTTACAGCGGAATGAAAATTTTTACTATACTCAAt                                                                                                                                                                          <  1:607051/98‑1 (MQ=255)                                                                                                                                                                                                                                    gtgtTGATGTGTAATGTGTCTAAATCCTGAAAGTAATTGAGTAATGTCATGGCGGAACCCCTTACAGCGGAATGAAAATTTTTACTATACTCAATCCGCCCCATAAATTTCACCTAAAATTAAGATTTTGCGGCTTATATCACGATCTTTGTTTTACAGAATGTAAAATTTAAAATACAATCTCCGTCAACTTTTAAATACACAATAAGGAAAATCTATGAACAAAACCGTAGGAAGTACGCTGTTAGTg               <  1:270519/250‑1 (MQ=255)                                                                                                                                                                                                                                     tgtTGATGTGTAATGTGTCTAAATCCTGAAAGTAATTGAGTAATGTCATGGCGGAACCCCTTACAGCGGAATGAAAATTTTTACTATACTCAATCCGCCCCATAAATTTCACCTAAAATTAAGATTTTGCGGCTTATATCACGATCTTTGTTTTACAGAATGTAAAATTTAAAATACAATCTCCGTCAACTTTTAAATACACAATAAGGAAAATCTATGAACAAAACCGTAGGAAGTACGCTGTTAGTGGc             <  1:847463/251‑1 (MQ=255)                                                                                                                                                                                                                                      gtTGATTTGTAATGTGTCTAAATCCTGAAAGGAATTGAGTAATGTCATGGCGGAAACCCTTACAGCGGAATGAAAATTTTTACTATACTCAATCCGCCCCATAAATTTAACCTAAAATTAAGATTTTGGGGCTTATATCACGATCTTTGTTTTACAGAATGTAAAATTTAAAATACAATCTCCGGCAACTTTTAAATACACAATAAGGAAAATATATTAACAAAACCGTAGGAAGTACCCTGTTAGTggaa            >  1:304189/1‑249 (MQ=255)                                                                                                                                                                                                                                      gtTGATGTGTAATGTGTCTAAATCCTGAAAGTAATTGAGTAATGTCATGGCGGAACCCCTTACAGCGGAATGAAAATTTTTACTATACTCAATCCGCCCCATAAATTTCACCTAAAATTAAGAt                                                                                                                                           >  1:31871/1‑124 (MQ=255)                                                                                                                                                                                                                                      gtTGATGTGTAATGTGTCTAAATCCTGAAAGTAATTGAGTAATGTCATGGCGGAACCCCTTACAGCGGAATGAAAATTTTTACTATACTCAATCCGCCCCATAAATTTCACCTAAAATTAAGATTTTGCGGCTTATATCACGATCTTTGTTTTACAg                                                                                                          >  1:181322/1‑157 (MQ=255)                                                                                                                                                                                                                                      gtTGATGTGTAATGTGTCTAAATCCTGAAAGTAATTGAGTAATGTCATGGCGGAACCCCTTACAGCGGAATGAAAATTTTTACTATACTCAATCCGCCCCATAAATTTCACCTAAAATTAAGATTTTGCGGCTTATATCACGATCTTTGTTTTACAGAATGTAAAATTTAAAATACAATCTCCGTCAACTTTTAAATACACAATaa                                                         <  1:331925/206‑1 (MQ=255)                                                                                                                                                                                                                                      gtTGATGTGTAATGTGTCTAAATCCTGAAAGTAATTGAGTAATGTCATGGCGGAACCCCTTACAGCGGAATGAAAATTTTTACTATACTCAATCCGCCCCATAAATTTCACCTAAAATTAAGATTTTGCGGCTTATATCACGATCTTTGTTTTACAGAATGTAAAATTTAAAATACAATCTCCGTCAACTTTTAAATACACAATAAGGAAAATCTATGAACAAAACCGTAGGAAGTACGCTGTTa                  <  1:150907/245‑1 (MQ=255)                                                                                                                                                                                                                                          aTGTGTAATGTGTCTAAATCCTGAAAGTAATTGAGTAATGTCATGGCGGAACCCCTTACAGCGGAATGAAAATTTTTACTATACTCAATCCGCCCCATAAATTTCACCTAAAATTAAGATTTTGCGGCTTATATCACGATCTTTGTTTTACAGAATGTAAAATTTAAAATACAATCTCCGTCAACTTTTAAATACACAATAAGGa                                                      <  1:388532/205‑1 (MQ=255)                                                                                                                                                                                                                                           tgtgTAATGTGTCTAAATCCTGAAAGTAATTGAGTAATGTCATGGCGGAACCCCTTACAGCGGAATGAAAATTTTTACTATACTCAATCCGCCCCATAAATTTCACCTAAAATTAAGATTTTGCGGCTTATATCACGATCTTTGTTTTACAg                                                                                                          >  1:889449/1‑152 (MQ=255)                                                                                                                                                                                                                                           tgtgTAATGTGTCTAAATCCTGAAAGTAATTGAGTAATGTCATGGCGGAACCCCTTACAGCGGAATGAAAATTTTTACTATACTCAATCCGCCCCATAAATTTCACCTAAAATTAAGATTTTGCGGCTTATATCACGATCTTTGTTTTACAGAATGTAAAATTTAAAATACAATCTCCGTCAACTTTTAAATACACAATAAGGAAAATCTATGAACAAAACCGTAGGAAGTACGCTGTTAGTGGCAGGaa        >  1:663297/1‑250 (MQ=255)                                                                                                                                                                                                                                           tgtgTAATGTGTCTAAATCCTGAAAGTAATTGAGTAATGTCATGGCGGAACCCCTTACAGCGGAATGAAAATTTTTACTATACTCAATCCGCCCCATAAATTTCACCTAAAATTAAGATTTTGCGGCTTATATCACGATCTTTGTTTTACAGAATGTAAAATTTAAAATACAATCTCCGTCAACTTTTAAATACACAATAAGGAAAATCTATGAACAAAACCGTAGGAAGTACGCTGTTAGTGGCAGGaa        >  1:66136/1‑250 (MQ=255)                                                                                                                                                                                                                                                aaTGTGTCTAAATCCTGAAAGTAATTGAGTAATGTCATGGCGGAACCCCTTACAGCGGAATGAAAATTTTTACTATACTCAATCCGCCCCATAAATTTCACCTAAAATTAAGATTTTGCGGCTTATATCACGATCTTTGTTTTACAGAATGTAAAATTTAAAATACAATCTCCGTCAACTTTTAAATACACAATAAGGAAAATCTATGAACAAAACCGTAGGAAGTACGCTGTTAGTGGCAGGAACCATGa  <  1:316482/251‑1 (MQ=255)                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                      ATCCTGTTCCTCCAAATAACTGCCGTCACACCATTTCAGCACGATAATATCGAGGATATTCTCGTCTAACTGTAAATAATTCGTTACATCAAATTCAGAGGTGCAATGGCTGATTTGGCTATAACCCACGAATTGCCCGTTCAACACCATAAAATAATCGTAAAATTCTCTTGGATTTTCCACCGCACTTTGGTCATTGGCATGGGGAATAAAATAGGCGTGTTGCGGTGTTGATGTGTAATGTTTCTAAATCCTGAAAGTAATTGAGTAATGTCATGGCGGAACCCCTTACAGCGGAATGAAAATTTTTACTATACTCAATCCGCCCCATAAATTTCACCTAAAATTAAGATTTTGCGGCTTATATCACGATCTTTGTTTTACAGAATGTAAAATTTAAAATACAATCTCCGTCAACTTTTAAATACACAATAAGGAAAATCTATGAACAAAACCGTAGGAAGTACGCTGTTAGTGGCAGGAACCATGA  >  1/1562071‑1562560

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑