Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,553,403	T→C	intergenic (‑108775/‑88361)	unknown ← / → unknown	tRNA‑Met‑CAT/tRNA‑Gly‑GCC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,553,403	0	T	C	100.0%	206.8 / NA	75	intergenic (‑108775/‑88361)	unknown/unknown	tRNA‑Met‑CAT/tRNA‑Gly‑GCC
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (35/40);  total (35/40)

ATGTTGCATTCGGCGCGAATATTACGTACGGCGACGATGACCTCTTTCAACCATGCGATTTCGGCTTCCGCGTCCACATCAAACGCCGTCATGTCCACTTGCGGGAACGGCTGCAACATAATGCTGTCGGCGTCAATGCCGACGAAACCTTTCACTTTCTGCCAGATTTCTTCGGTAATGAATGGAATAACCGGGTGCGCTAAACGCAGTAATTTCTCTAACACATGCACCAAGGTCTGGCTTGTCGCGCGAATTTGCGCTTGGTTGCCGTTTGCGAATACCGGCTTGGTGAGTTCCAAATACCAGTCACAGAATTGGTTCCAAGTGAATTCATAAAGCGCGGTGGCACATAAGTCAAAACGGAATTGGCTTAATGCGTTGCGGAAGGTTTCCACAGTGCGGTTGAATTCCGATTGGATCCAACGATCGGCAACGGAAAATTCAATTTCGCCTTGGCTTAAATCCAGCTTTTCATTGGTAAGCACGAAGC  >  1/1553159‑1553648                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                      aTGTTGCATTCGGCGCGAATATTACGTACGGCGACGATGACCTCTTTCAACCATGCGATTTCGGCTTCCGCGTCCACATCAAACGCCGTCATGTCCACTTGCGGGAACGGCTGCAACATAATGCTGTCGGCGTCAATGCCGACGAAACCTTTCACTTTCTGCCAGATTTCTTCGGTAATGAATGGAATAACCGGGTGCGCTAAACGCAGTAATTTCTCTAACACATGCACCAAGGTCTGGCTTGCcgcgcg                                                                                                                                                                                                                                                 >  1:257372/1‑251 (MQ=255)                    tatTACGTACGGCGACGATGACCTCTTTCAACCATGCGATTTCGGCTTCCGCGTCCACATCAAACGCCGTCATGTCCACTTGCGGGAACGGCTGCAACATAATGCTGTCGGCGTCAATGCCGACGAAACCTTTCACTTTCTGCCAGATTTCTTCGGTAATGAATGGAATAACCGGGTGCGCTAAACGCAGTAATTTCTCTAACACATGCACCAAGGTCTGGCTTGCCGCGCGAATTTGCGCTTGGTTGCCg                                                                                                                                                                                                                              <  1:87643/251‑1 (MQ=255)                     atTACGTACGGCGACGATGACCTCTTTCAACCATGCGATTTCGGCTTCCGCGTCCACATCAAACGCCGTCATGTCCACTTGCGGGAACGGCTGCAACATAATGCTGTCGGCGTCAATGCCGACGAAACCTTTCACTTTCTGCCAGATTTCTTCGGTAATGAATGGAATAACCGGGTGCGCTAAACGCAGTAATTTCTCTAACACATGCACCAAGGTCTGGCTTGCCGCGCGAATTTGCGCTTGGTTGCCGt                                                                                                                                                                                                                             >  1:216305/1‑251 (MQ=255)                     atTACGTACGGCGACGATGACCTCTTTCAACCATGCGATTTCGGCTTCCGCGTCCACATCAAACGCCGTCATGTCCACTTGCGGGAACGGCTGCAACATAATGCTGTCGGCGTCAATGCCGACGAAACCTTTCACTTTCTGCCAGATTTCTTCGGTAATGAATGGAATAACCGGGTGCGCTAAACGCAGTAATTTCTCTAACACATGCACCAAGGTCTGGCTTGCCGCGCGAATTTGCGCTTGGTTGCCGt                                                                                                                                                                                                                             >  1:750722/1‑251 (MQ=255)                                         ccTCTTTCAACCATGCGATTTCGGCTTCCGCGTCCACATCAAACGCCGTCATGTCCACTTGCGGGAACGGCTGCAACATAATGCTGTCGGCGTCAATGCCGACGAAACCTTTCACTTTCTGCCAGATTTCTTCGGTAATGAATGGAATAACCGGGTGCGCTAAACGCAGTAATTTCTCTAACACATGCACCAAGGTCTGGCTTGCCGCGCGAATTTGCGCTTGGTTGCCGTTTGCGAATACCGGCTTGGTg                                                                                                                                                                                                         <  1:580367/251‑1 (MQ=255)                                          gtctTTCAACCATGCGATTTCGGCTTCCGCGTCCACATCAAACGCCGTCATGTCCACTTGCGGGAACGGCTGCAACATAATGCTGTCGGCGTCAATGCCGACGAAACCTTTCACTTTCTGCCAGATTTCTTCGGTAATGAATGGAATAACCGGGTGCGCTAAACGCAGTAATTTCTCTAACACATGCACCAAGGTCTGGCTTGCCGCGCGAATTTGCGCTTGGTTGCCGTTTGCGAATACCGGCTTGGTGa                                                                                                                                                                                                        >  1:141700/2‑251 (MQ=255)                                          ctctTTCAACCATGCGATTTCGGCTTCCGCGTCCACATCAAACGCCGTCATGTCCACTTGCGGGAACGGCTGCAACATAATGCTGTCGGCGTCAATGCCGACGAAACCTTTCACTTTCTGCCAGATTTCTTCGGTAATGAATGGAATAACCGGGTGCGCTAAACGCAGTAATTTCTCTAACACATGCACCAAGGTCTGGCTTGCCGCGCGAATTTGCGCTTGGTTGCCGTTTGCGAATACCGGCTTGGTgg                                                                                                                                                                                                        >  1:116118/1‑250 (MQ=255)                                           tctTTCAACCATGCGATTTCGGCTTCCGCGTCCACATCAAACGCCGTCATGTCCACTTGCGGGAACGGCTGCAACATAATGCTGTCGGCGTCAATGCCGACGAAACCTTTCACTTTCTGCCAGATTTCTTCGGTAATGAATGGAATAACCGGGTGCGCTAAACGCAGTAATTTCTCTAACACATGCACCAAGGTCTGGCTTGCCGCGCGAATTTGCGCTTGGTTGCCGTTTGCGAATACCGGCTTGGTGAg                                                                                                                                                                                                       >  1:542207/1‑251 (MQ=255)                                            ctTTCAGCCATGCGATTTCGGCTTCCGCGTCCACATCAAACGCCGTCATGTCCACTTGCGGGAACTACTGCAACATAATGCTGTCGGCGTCAATGCCGACGTAACCTTTCACTTTCGGCCAGATTTCTTCGGTAATGAATGGAATAACCGGGAGCGCTAAGCGCAGTAATTTCTCTAACACATGCACCAGGGTCTGGCTTGCCGCGCGAATTTgcgc                                                                                                                                                                                                                                        <  1:73730/217‑1 (MQ=255)                                                   ccATGCGATTTCGGCTTCCGCGTCCACATCAAACGCCGTCATGTCCACTTGCGGGAACGGCTGCAACATAATGCTGTCGGCGTCAATGCCGACGAAACCTTTCACTTTCTGCCAGATTTCTTCGGTAATGAATGGAATAACCGGGTGCGCTAAACGCAGTAATTTCTCTAACACATGCACCAAGGTCTGGCTTGCCGCGCGAATTTGCGCTTGGTTGCCGTTTGCGAATACCGGCTTGGTGAGTTCCAAAt                                                                                                                                                                                               <  1:361602/251‑1 (MQ=255)                                                   ccATGCGATTTCGGCTTCCGCGTCCACATCAAACGCCGTCATGTCCACTTGCGGGAACGGCTGCAACATAATGCTGTCGGCGTCAATGCCGACGAAACCTTTCACTTTCTGCCAGATTTCTTCGGTAATGAATGGAATAACCGGGTGCGCTAAACGCAGTAATTTCTCTAACACATGCACCAAGGTCTGGCTTGCCGCGCGAATTTGCGCTTGGTTGCCGTTTGCGAATACCGGCTTGGTGAGTTCCAAAt                                                                                                                                                                                               <  1:276673/251‑1 (MQ=255)                                                      tGCGATTACGGCTTCCGCGTCCACATCAAACGCCGTCATGTCCACTTGCGGGAACGGCTGCAACATAATGCTGTCGGCGTCAATGCCGACGAAACCTTTCACTTTCTGCCAGATTTCTTCGGTAATGAATGGAATAACCGGGTGCGCTAAACGCAGTAATTTCTCTAACACATGCACCAAGGTCTGGCTTGCCGCGCGAATTTGCGCTTGGTTGCCGTTTGCGAATACCGGCTTGGTGAGTTCCAAATAcc                                                                                                                                                                                            <  1:24389/251‑1 (MQ=255)                                                         gATTTCGGCTTCCGCGTCCACATCAAACGCCGTCATGTCCACTTGCGGGAACGGCTGCAACATAATGCTGTCGGCGTCAATGCCGACGAAACCTTTCACTTTCTGCCAGATTTCTTCGGTAATGAATGGAATAACCGGGTGCGCTAAACGCAGTAATTTCTCTAACACATGCACCAAGGTCTGGCTTGCCGCGCGAATTTGCGCTTGGTTGCCGTTTGCGAATACCGGCTTGGTGAGTTCCAAATACCAGt                                                                                                                                                                                         >  1:586949/1‑251 (MQ=255)                                                                 cTTCCGCGTCCACATCAAACGCCGTCATGTCCACTTGCGGGAACGGCTGCAACATAATGCTGTCGGCGTCAATGCCGACGAAACCTTTCACTTTCTGCCAGATTTCTTCGGTAATGAATGGAATAACCGGGTGCGCTAAACGCAGTAATTTCTCTAACACATGCACCAAGGTCTGGCTTGCCGCGCGAATTTGCGCTTGGTTGCCGTTTGCGAATACCGGCTTGGTGAGTTCCAAATACCAGTCACAGAAt                                                                                                                                                                                 <  1:756024/251‑1 (MQ=255)                                                                 cTTCCGCGTCCACATCAAACGCCGTCATGTCCACTTGCGGGAACGGCTGCAACATAATGCTGTCGGCGTCAATGCCGACGAAACCTTTCACTTTCTGCCAGATTTCTTCGGTAATGAATGGAATAACCGGGTGCGCTAAACGCAGTAATTTCTCTAACACATGCACCAAGGTCTGGCTTGCCGCGCGAATTTGCGCTTGGTTGCCGTTTGCGAATACCGGCTTGGTGAGTTCCAAATACCAGTCACAGAAt                                                                                                                                                                                 <  1:430751/251‑1 (MQ=255)                                                                       cgTCCACATCAAACGCCGTCATGTCCACTTGCGGGAACGGCTGCAACATAATGCTGTCGGCGTCAATGCCGACGAAACCTTTCACTTTCTGCCAGATTTCTTCGGTAATGAATGGAATAACCGGGTGCGCTAAACGCAGTAATTTCTCTAACACATGCACCAAGGTCTGGCTTGCCGCGCGAATTTGCGCTTGGTTGCCGTTTGCGAATACCGGCTTGGTGAGTTCCAAATACCAGTCACAGAATTGGTTc                                                                                                                                                                           <  1:740462/251‑1 (MQ=255)                                                                        gTCCACATCAAACGCCGTCATGTCCACTTGCGGGAACGGCTGCAACATAATGCTGTCGGCGTCAATGCCGACGAAACCTTTCACTTTCTGCCAGATTTCTTCGGTAATGAATGGAATAACCGGGTGCGCTAAACGCAGTAATTTCTCTAACACATGCACCAAGGTCTGGCTTGCCGCGCGAATTTGCGCTTGGTTGCCGTTTGCGAATACCGGCTTGGTGAGTTCCAAATACCAGTCACAGAATTGGTTc                                                                                                                                                                           >  1:377339/1‑250 (MQ=255)                                                                             caTCAAACGCCGTCATGTCCACTTGCGGGAACGGCTGCAACATAATGCTGTCGGCGTCAATGCCGACGAAACCTTTCACTTTCTGCCAGATTTCTTCGGTAATGAATGGAATAACCGGGTGCGCTAAACGCAGTAATTTCTCTAACACATGCACCAAGGTCTGGCTTGCCGCGCGAATTTGCGCTTGGTTGCCGTTTGCGAATACCGGCTTGGTg                                                                                                                                                                                                         <  1:271529/215‑1 (MQ=255)                                                                             caTCAAACGCCGTCATGTCCACTTGCGGGAACGGCTGCAACATAATGCTGTCGGCGTCAATGCCGACGAAACCTTTCACTTTCTGCCAGATTTCTTCGGTAATGAATGGAATAACCGGGTGCGCTAAACGCAGTAATTTCTCTAACACATGCACCAAGGTCTGGCTTGCCGCGCGAATTTGCGCTTGGTTGCCGTTTGCGAATACCGGCTTGGTGAGTTCCAAATACCAGTCACAGAATTGGTTCCAAGTg                                                                                                                                                                     <  1:532930/251‑1 (MQ=255)                                                                                  aaCGCCGTCATGTCCACTTGCGGGAACGGCTGCAACATAATGCTGTCGGCGTCAATGCCGACGAAACCTTTCACTTTCTGCCAGATTTCTTCGGTAATGAATGGAATAACCGGGTGCGCTAAACGCAGTAATTTCTCTAACACATGCACCAAGGTCTGGCTTGCCGCGCGAATTTGCGCTTGGTTGCCGTTTGCGAATACCGGCTTGGTGAGTTCCAAATACCAGTCACAGAATTGGTTCCAAGTGAATTc                                                                                                                                                                <  1:858917/251‑1 (MQ=255)                                                                                         tCATGTCCACTTGCGGGAACGGCTGCAACATAATGCTGTCGGCGTCAATGCCGACGAAACCTTTCACTTTCTGCCAGATTTCTTCGGTAATGAATGGAATAACCGGGTGCGCTAAACGCAGTAATTTCTCTAACACATGCACCAAGGTCTGGCTTGCCGCGCGAATTTGCGCTTGGTTGCCGTTTGCGAATACCGGCTTGGTGAGTTCCAAATACCAGTCACAGAATTGGTTCCAAGTGAATTCATAAAg                                                                                                                                                          <  1:504231/250‑1 (MQ=255)                                                                                          cATGTCCACTTGCGGGAACGGCTGCAACATAATGCTGTCGGCGTCAATGCCGACGAAACCTTTCACTTTCTGCCAGATTTCTTCGGTAATGAATGGAATAACCGGGTGCGCTAAACGCAGTAATTTCTCTAACACATGCACCAAGGTCTGGCTTGCCGCGCGAATTTGCGCTTGGTTGCCGTTTGCGAATACCGGCTTGGTGAGTTCCAAATACCAGTCACAGAATTGGtt                                                                                                                                                                            <  1:466477/231‑1 (MQ=255)                                                                                             gTCCACTTGCGGGAACGGCTGCAACATAATGCTGTCGGCGTCAATGCCGACGAAACCTTTCACTTTCTGCCAGATTTCTTCGGTAATGAATGGAATAACCGGGTGCGCTAAACGCAGTAATTTCTCTAACACATGCACCAAGGTCTGGCTTGCCGCGCGAATTTGCGCTTGGTTGCCGTTTGCGAATACCGGCTTGGTGAGTTCCAAATACCAGTCACAGAATTGGTTCCAAGTGAATTCATAAAGCGCgg                                                                                                                                                     >  1:193391/1‑251 (MQ=255)                                                                                                  cTTGCGGGAACGGCTGCAACATAATGCTGTCGGCGTCAATGCCGACGAAACCTTTCACTTTCTGCCAGATTTCTTCGGTAATGAATGGAATAACCGGGTGCGCTAAACGCAGTAATTTCTCTAACACATGCACCAAGGTCTGGCTTGCCGCGCGAATTTGCGCTTGGTTGCCGTTTGCGAATACCGGCTTGGTGAGTTCCAAATAc                                                                                                                                                                                             >  1:652606/1‑206 (MQ=255)                                                                                                          aaCGGCTGCAACATAATGCTGTCGGCGTCAATGCCGACGAAACCTTTCACTTTCTGCCAGATTTCTTCGGTAATGAATGGAATAACCGGGTGCGCTAAACGCAGTAATTTCTCTAACACATGCACCAAGGTCTGGCTTGCCGCGCGAATTTGCGCTTGGTTGCCGTTTGCGAATACCGGCTTGGTGAGTTCCAAATACCAGTCACAGAATTGGTTCCAAGTGAATTCATAAAGCGCGGTGGCACATAAGt                                                                                                                                         <  1:671304/250‑1 (MQ=255)                                                                                                               cTGCAACATAATGCTGTCGGCGTCAATGCCGACGAAACCTTTCACTTTCTGCCAGATTTCTTCGGTAATGAATGGAATAACCGGGTGCGCTAAACGCAGTAATTTCTCTAACACATGCACCAAGGTCTGGCTTGCCGCGCGAATTTGCGCTTGGTTGCCGTTTGCGAATACCGGCTTGGTGAGTTCCAAATACCAGTCACAGAATTGGTTCCAAGt                                                                                                                                                                      <  1:230420/216‑1 (MQ=255)                                                                                                               cTGCAACATAATGCTGTCGGCGTCAATGCCGACGAAACCTTTCACTTTCTGCCAGATTTCTTCGGTAATGAATGGAATAACCGGGTGCGCTAAACGCAGTAATTTCTCTAACACATGCACCAAGGTCTGGCTTGCCGCGCGAATTTGCGCTTGGTTGCCGTTTGCGAATACCGGCTTGGTGAGTTCCAAATACCAGTCACAGAATTGGTTCCAAGTGAATTCATAAAGCGCGGTGGCACAt                                                                                                                                             <  1:514795/241‑1 (MQ=255)                                                                                                                    aCATAATGCTGTCGGCGTCAATGCCGACGAAACCTTTCACTTTCTGCCAGATTTCTTCGGTAATGAATGGAATAACCGGGTGCGCTAAACGCAGTAATTTCTCTAACACATGCACCAAGGTCTGGCTTGCCGCGCGAATTTGCGCTTGGTTGCCGTTTGCGAATACCGGCTTGGTGAGTTCCAAATACCAGTCACAGAATTGGTTCCAAGTGAATTCATAAAGCGCGGTGGCACATAAGTCAAAACGGAAt                                                                                                                              >  1:179169/1‑251 (MQ=255)                                                                                                                    aCATAATGCTGTCGGCGTCAATGCCGACGAAACCTTTCACTTTCTGCCAGATTTCTTCGGTAATGAATGGAATAACCGGGTGCGCTAAACGCAGTAATTTCTCTAACACATGCACCAAGGTCTGGCTTGCCGCGCGAATTTGCGCTTGGTTGCCGTTTGCGAATACCGGCTTGGTGAGTTCCAAATACCAGTCACAGAATTGGTTCCAAGTGAATTCATAAAGCGCGGTGGCACATAAGTCAAAACGGAAt                                                                                                                              >  1:519839/1‑251 (MQ=255)                                                                                                                            cTGTCGGCGTCAATGCCGACGAAACCTTTCACTTTCTGCCAGATTTCTTCGGTAATGAATGGAATAACCGGGTGCGCTAAACGCAGTAATTTCTCTAACACATGCACCAAGGTCTGGCTTGCCGCGCGAATTTGCGCTTGGTTGCCGTTTGCGAATACCGGCTTGGTGAGTTCCAAATACCAGTCACAGAATTGGTTCCAAGTGAATTCATAAAGCGCGGTGGCACATAAGTCAAAACGGAATTGGCTTaa                                                                                                                      >  1:399780/1‑251 (MQ=255)                                                                                                                               tCGGCGTCAATGCCGACGAAACCTTTCACTTTCTGCCAGATTTCTTCGGTAATGAATGGAATAACCGGGTGCGCTAAACGTAGTAATTTCTCTAACACATGCACCAAGGTCTGGCTTGCCGCGCGAATTTGCGCTTGGTTGCCGTTTGCGAATACCGGCTTGGTGAGTTCCAAATACCAGTCACAGAATTGGTTCCAAGTGAATTCATAAAGCGCGGTGGCACATAAGTCAAAACGGAATTGGCTTAATg                                                                                                                    <  1:445963/250‑1 (MQ=255)                                                                                                                               tCGGCGTCAATGCCGACGAAACCTTTCACTTTCTGCCAGATTTCTTCGGTAATGAATGGAATAACCGGGTGCGCTAAACGCAGTAATTTCTCTAACACATGCACCAAGGTCTGGCTTGCCGCGCGAATTTGCGCTTGGTTGCCGTTTGCGAATACCGGCTTGGTGAGTTCCAAATACCAGTCACAGAATTGGTTCCAAGTGAATTCATAAAGCGCGGTGGCACATAAGTCAAAACGGAATTGGCTTAATg                                                                                                                    <  1:276336/250‑1 (MQ=255)                                                                                                                               tCGGCGTCAATGCCGACGAAACCTTTCACTTTCTGCCAGATTTCTTCGGTAATGAATGGAATAACCGGGTGCGCTAAACGCAGTAATTTCTCTAACACATGCACCAAGGTCTGGCTTGCCGCGCGAATTTGCGCTTGGTTGCCGTTTGCGAATACCGGCTTGGTGAGTTCCAAATACCAGTCACAGAATTGGTTCCAAGTGAATTCATAAAGCGCGGTGGCACATAAGTCAAAACGGAATTGGCTTAATGc                                                                                                                   <  1:698617/251‑1 (MQ=255)                                                                                                                                        aTGCCGACGAAACCTTTCACTTTCTGCCAGATTTCTTCGGTAATGAATGGAATAACCGGGTGCGCTAAACGCAGTAATTTCTCTAACACATGCACCAAGGTCTGGCTTGCCGCGCGAATTTGCGCTTGGTTGCCGTTTGCGAATACCGGCTTGGTGAGTTCCAAATACCAGTCACAGAATTGGTTCCAAGTGAATTCATAAAGCGCGGTGGCACATAAGTCAAAACGGAATTGGCTTAATg                                                                                                                    >  1:262526/1‑241 (MQ=255)                                                                                                                                                 aaaCCTTTCACTTTCTGCCAGATTTCTTCGGTAATGAATGGAATAACCGGGTGCGCTAAACGCAGTAATTTCTCTAACACATGCACCAAGGTCTGGCTTGCCGCGCGAATTTGCGCTTGGTTGCCGTTTGCGAATACCGGCTTGGTGAGTTCCAAATACCAGTCACAGAATTGGTTCCAAGTGAATTCATAAAGCGCGGTGGCACATAAg                                                                                                                                          >  1:716073/1‑210 (MQ=255)                                                                                                                                                 aaaCCTTTCACTTTCTGCCAGATTTCTTCGGTAATGAATGGAATAACCGGGTGCGCTAAACGCAGTAATTTCTCTAACACATGCACCAAGGTCTGGCTTGCCGCGCGAATTTGCGCTTGGTTGCCGTTTGCGAATACCGGCTTGGTGAGTTCCAAATACCAGTCACAGAATTGGTTCCAAGTGAATTCATAAAGCGCGGTGGCACATAAGTCAAAACGGAATTGGCTTAATGCGTTGCGGAAGGTTTCcac                                                                                                 <  1:43912/251‑1 (MQ=255)                                                                                                                                                 aaaCCTTTCACTTTCTGCCAGATTTCTTCGGTAATGAATGGAATAACCGGGTGCGCTAAACGCAGTAATTTCTCTAACACATGCACCAAGGTCTGGCTTGCCGCGCGAATTTGCGCTTGGTTGCCGTTTGCGAATACCGGCTTGGTGAGTTCCAAATACCAGTCACAGAATTGGTTCCAAGTGAATTCATAAAGCGCGGTGGCACATAAGTCAAAACGGAATTGGCTTAATGCGTTGCGGAAGGTTTCcac                                                                                                 <  1:477255/251‑1 (MQ=255)                                                                                                                                                   aCCTTTCACTTTCTGCCAGATTTCTTCGGTAATGAATGGAATAACCGGGTGCGCTAAACGCAGTAATTTCTCTAACACATGCACCAAGGTCTGGCTTGCCGCGCGAATTTGCGCTTGGTTGCCGTTTGCGAATACCGGCTTGGTGAGTTCCAAATACCAGTCACAGAATTGGTTCCAAGTGAATTCATAAAGCGCGGTGGCACATAAGTCAAAACGGAATTGGCTTAATGCGTTGCGGAAGGTTTCCACAg                                                                                               <  1:631998/251‑1 (MQ=255)                                                                                                                                                    ccTTTCACTTTCTGCCGGATTTCTTCGGTAATGAATGGAATAACCGGGTGCGCTAAACGCAGTAATTTCTCTAACACATGCACCAAGGTCTGGCTTGCCGCGCGAATTTGCGCTTGGTTGCCGTTTGCGAATACCGGCTTGGTGAGTTCCAAATACCAGTCACAGAATTGGTTCCAAGTGAATTCATAAAGCGCGGTGGCACATAAGTCAAAACGGAATTGGCt                                                                                                                         >  1:488106/1‑224 (MQ=255)                                                                                                                                                    ccTTTCACTTTCTGCCAGATTTCTTCGGTAATGAATGGAATAACCGGGTGCGCTAAACGCAGTAATTTCTCTAACACATGCACCAAGGTCTGGCTTGCCGCGCGAATTTGCGCTTGGTTGCCGTTTGCGAATACCGGCTTGGTGAGTTCCAAATACCAGTCACAGAATTGGTTCCAAGTGAATTCATAAAGCGCGGTGGCACAt                                                                                                                                             >  1:483869/1‑204 (MQ=255)                                                                                                                                                    ccTTTCACTTTCTGCCAGATTTCTTCGGTAATGAATGGAATAACCGGGTGCGCTAAACGCAGTAATTTCTCTAACACATGCACCAAGGTCTGGCTTGCCGCGCGAATTTGCGCTTGGTTGCCGTTTGCGAATACCGGCTTGGTGAGTTCCAAATACCAGTCACAGAATTGGTTCCAAGTGAATTCATAAAGCGCGGTGGCACATAAGTCAAAACGGAATTGGCt                                                                                                                         >  1:171526/1‑224 (MQ=255)                                                                                                                                                     cTTTCACTTTCTGCCAGATTTCTTCGGTAATGAATGGAATAACCGGGTGCGCTAAACGCAGTAATTTCTCTAACACATGCACCAAGGTCTGGCTTGCCGCGCGAATTTGCGCTTGGTTGCCGTTTGCGAATACCGGCTTGGTGAGTTCCAAATACCAGTCACAGAATTGGTTCCAAGTGAATTCATAAAGCGCGGTGGCACATAAGTCAAAACGGAATTGGCTTAATGCGTTGCGGAAGGTTTCCACAGTg                                                                                             <  1:323421/251‑1 (MQ=255)                                                                                                                                                      tttCACTTTCTGCCAGATTTCTTCGGTAATGAATGGAATAACCGGGTGCGCTAAACGCAGTAATTTCTCTAACACATGCACTAAGGTCTGGCTTGCCGCGCGAATTTGCGCTTGGTTGCCGTTTGCGAATACCGGCTTGGTGAGTTCCAAATACCAGTCACAGAATTGGTTCCAAGTGAATTCATAAAGCGCGGTGGCACATAAGTCAAAACGGAATTGGCTTAATGCGTTGCGGAAGGTTTCCACAGTGc                                                                                            <  1:433681/251‑1 (MQ=255)                                                                                                                                                               cTGCCAGATTTCTTCGGTAATGAATGGAATAACCGGGTGCGCTAAACGCAGTAATTTCTCTAACACATGCACCAAGGTCTGGCTTGCCGCGCGAATTTGCGCTTGGTTGCCGTTTGCGAATACCGGCTTGGTGAGTTCCAAATACCAGTCACAGAATTGGTTCCAAg                                                                                                                                                                       >  1:326717/1‑167 (MQ=255)                                                                                                                                                                     gATTTCTTCGGTAATGAATGGAATAACCGGGTGCGCTAAACGCAGTAATTTCTCTAACACATGCACCAAGGTCTGGCTTGCCGCGCGAATTTGCGCTTGGTTGCCGTTTGCGAATACCGGCTTGGTGAGTTCCAAATACCAGTAACAGAATTGGTTCCAAGTGAATTCATAAAGCGCGGTGGCACATAAGTCAAAACGGAATTGGCTTAATGCGTTGCGGAAGGTTTCCACAGTGCGGTTGAATTCCGAtt                                                                             >  1:582002/1‑251 (MQ=255)                                                                                                                                                                      aTTTCTTCGGTAATGAATGGAATAACCGGGTGCGCTAAACGCAGTAATTTCTCTAACACATGCACCAAGGTCTGGCTTGCTGCGCGAATTTGCGCTTGGTTGCCGTTTGCGAATACCGGCTTGGTGAGTTCCAAATACCAGTCACAGAATTGGTTCCAAGTGAATTCATAAAGCGCGGTGGCACATAAGTCAAAACGGAATTGGCTTAATGCGTTGCGGAAGGTTTCCACAGTGCGGTTGAATTCCGAtt                                                                             <  1:432908/250‑1 (MQ=255)                                                                                                                                                                                tAATGAATGGAATAACCGGGTGCGCTAAACGCAGTAATTTCTCTAACACATGCACCAAGGTCTGGCTTGCCGCGCGAATTTGCGCTTGGTTGCCGTTTGCGAATACCGGCTTGGTGAGTTCCAAATACCAGTCACAGAATTGGTTCCAAGTGAATTCATAAAGCGCGGTGGCACATAAGTCAAAACGGAATTGGCTTAATGCGTTGCGGAAGGTTTCCACAGTGCGGTTGAATTCCGATTGGATCCAACg                                                                   <  1:316380/250‑1 (MQ=255)                                                                                                                                                                                     aatgGAATAACCGGGTGCGCTAAACGCAGTAATTTCTCTAACACATGCACCAAGGTCTGGCTTGCCGCGCGAATTTGCGCTTGGTTGCCGTTTGCGAATACCGGCTTGGTGAGTTCCAAATACCAGTCACAGAATTGGTTCCAAGTGAATTCATAAAGCGCGGTGGCACATAAGTCAAAACGGAATTGGCTTAATGCGTTGCGGAAGGTTTCCACAGTGCGGTTGAATTCCGATTGGATCCAACGATCGGc                                                             <  1:330803/251‑1 (MQ=255)                                                                                                                                                                                         gAATAACCGGGTGCGCTAAACGCAGTAATTTCTCTAACACATGCACCAAGGTCTGGCTTGCCGCGCGAATTTGCGCTTGGTTGCCGTTTGCGAATACCGGCTTGGTGAGTTCCAAATACCAGTCACAGAATTGGTTCCAAGTGAATTCATAAAGCGCGGTGGCACATAAGTCAAAACGGAATTGGCTTAATGCGTTGCGGAAGGTTTCCACAGTGCGGTTGAATTCCGATTGGATCCAACGATCGGCAACg                                                         >  1:394837/1‑251 (MQ=255)                                                                                                                                                                                         gAATAACCGGGTGCGCTAAACGCAGTAATTTCTCTAACACATGCACCAAGGTCTGGCTTGCCGCGCGAATTTGCGCTTGGTTGCCGTTTGCGAATACCGGCTTGGTGAGTTCCAAATACCAGTCACAGAATTGGTTCCAAGTGAATTCATAAAGCGCGGTGGCACATAAGTCAAAACGGAATTGGCTTAATGCGTTGCGGAAGGTTTCCACAGTGCGGTTGAATTCCGATTGGATCCAACGATCGGCAACg                                                         >  1:490429/1‑251 (MQ=255)                                                                                                                                                                                         gAATAACCGGGTGCGCTAAACGCAGTAATTTCTCTAACACATGCACCAAGGTCTGGCTTGCCGCGCGAATTTGCGCTTGGTTGCCGTTTGCGAATACCGGCTTGGTGAGTTCCAAATACCAGTCACAGAATTGGTTCCAAGTGAATTCATAAAGCGCGGTGGCACATAAGTCAAAACGGAATTGGCTTAATGCGTTGCGGAAGGTTTCCACAGTGCGGTTGAATTCCGATTGGATCCAACGATCGGCAACg                                                         >  1:729485/1‑251 (MQ=255)                                                                                                                                                                                            tAACCGGGTGCGCTAAACGCAGTAATTTCTCTAACACATGCACCAAGGTCTGGCTTGCCGCGCGAATTTGCGCTTGGTTGCCGTTTGCGAATACCGGCTTGGTGAGTTCCAAATACCAGTCACAGAATTGGTTCCAAGTGAATTCATAAAGCGCGGTGGCACATAAGTCAAAACGGAATTGGCTTAATGCGTTGCGGAAGGTTTCCACAGTGCGGTTGAATTCCGATTGGATCCAACGATCGGCAACGGaa                                                      <  1:550275/251‑1 (MQ=255)                                                                                                                                                                                             aaCCGGGTGCGCTAAACGCAGTAATTTCTCTAACACATGCACCAAGGTCTGGCTTGCCGCGCGAATTTGCGCTTGGTTGCCGTTTGCGAATACCGGCTTGGTGAGTTCCAAATACCAGTCACAGAATTGGTTCCAAGTGAATTCATAAAGCGCGGTGGCACATAAGTCAAAACGGAATTGGCTTAATGCGTTGCGGAAGGTTTCcac                                                                                                 >  1:77157/1‑207 (MQ=255)                                                                                                                                                                                                  ggTGCGCTAAACGCAGTAATTTCTCTAACACATGCACCAAGGTCTGGCTTGCCGCGCGAATTTGCGCTTGGTTGCCGTTTGCGAATACCGGCTTGGTGAGTTCCAAATACCAGTCACAGAATTGGTTCCAAGTGAATTCATAAAGCGCGGTGGCACATAAGTCAAAACGGAATTGGCTTAATGCGTTGCGGAAGGTTTCCACAGTGCGGTTGAATTCCGATTGGATCCAACGATCGGCAACGGAAAATTc                                                 <  1:827889/250‑1 (MQ=255)                                                                                                                                                                                                     gcgcTAAACGCAGTAATTTCTCTAACACATGCACCAAGGTCTGGCTTGCCGCGCGAATTTGCGCTTGGTTGCCGTTTGCGAATACCGGCTTGGTGAGTTCCAAATACCAGTCACAGAATTGGTTCCAAGTGAATTCATAAAGCGCGGTGGCACATAAGTCAAAACGGAATTGGCTTAATGCGTTGCGGAAGGTTTCCACAGTGCGGTTGAATTCCGATTGGATCCAACGATCGGCAACGGAAAATTCAAtt                                             >  1:455365/1‑251 (MQ=255)                                                                                                                                                                                                         tAAACGCAGTAATTTCTCTAACACATGCACCGAGGTCTGGCTTGCCGCGCGAATTTGCGCTTGGTTGCCGTTTGCGAATACCGGCTTGGTGAGTTCCAAATACCAGTCACAGAATTGGTTCCAAGTGAATTCATAAAGCGCGGTGGCACATAAGTCAAAACGGAATTGGCTTAATGCGTTGCGGAAGGTTTCCACAGTGCGGTTGAATTCCGATTGGATCCAACGATCGGCAACGGAAAATTCAATTTCGc                                         <  1:647106/251‑1 (MQ=255)                                                                                                                                                                                                           aaCGCAGTAATTTCTCTAACACATGCACCAAGGTCTGGCTTGCCGCGCGAATTTGCGCTTGGTTGCCGTTTGCGAATACCGGCTTGGTGAGTTCCAAATACCAGTCACAGAATTGGTTCCAAGTGAATTCATAAAGCGCGGTGGCACATAAGTCAAAACGGAATTGGCTTAATGCGTTGCGGAAGGTTTCCACAGTGCGGTTGAATTCCGATTGGATCCAACGATCGGCAACGGAAAATTCAATTTCGCCt                                       <  1:321088/251‑1 (MQ=255)                                                                                                                                                                                                               cAGTAATTTCTCTAACACATGCACCAAGGTCTGGCTTGCCGCGCGAATTTGCGCTTGGTTGCCGTTTGCGAATACCGGCTTGGTGAGTTCCAAATACCAGTCACAGAATTGGTTCCAAGTGAATTCATAAAGCGCGGTGGCACATAAGTCAAAACGGAATTGGCTTAATGCGTTGCGGAAGGTTTCCACAGTGCGGTTGAATTCCGATTGGATCCAACGATCGGCAACGGAAAATTCAATTTCGCCTTgg                                    >  1:871757/1‑250 (MQ=255)                                                                                                                                                                                                                aGTAATTTCTCTAACACATGCACCAAGGTCTGGCTTGCCGCGCGAATTTGCGCTTGGTTGCCGTTTGCGAATACCGGCTTGGTGAGTTCCAAATACCAGTCACAGAATTGGTTCCAAGTGAATTCATAAAGCGCGGTGGCACATAAGTCAAAACGGAATTGGCTTAATGCGTTGCGGAAGGTTTCCACAGTGCGGTTGAATTCCGATTGGATCCAACGa                                                                  >  1:88812/1‑219 (MQ=255)                                                                                                                                                                                                                  tAATTTCTCTAACACATGCACCAAGGTCTGGCTTGCCGCGCGAATTTGCGCTTGGTTGCCGTTTGCGAATACCGGCTTGGTGAGTTCCAAATACCAGTCACAGAATTGGTTCCAAGTGAATTCATAAAGCGCGGTGGCACATAAGTCAAAACGGAATTGGCTTAATGCGTTGCGGAAGGTTTCCACAGTGCGGTTGAATTCCGATTGGATCCAACGATCGGCAACGGAAAATTCAATTTCGCCTTGGCt                                  <  1:846118/249‑1 (MQ=255)                                                                                                                                                                                                                   aaTTTCTCTAACACATGCACCAAGGTCTGGCTTGCCGCGCGAATTTGCGCTTGGTTGCCGTTTGCGAATACCGGCTTGGTGAGTTCCAAATACCAGTCACAGAATTGGTTCCAAGTGAATTCATAAAGCGCGGTGGCACATAAGTCAAAACGGAATTGGCTTAATGCGTTGCGGAAGGTTTCCACAGTGCGGTTGAATTCCGATTGGATCCAACGATCGGCAACGGAAAATTCAATTTCGCCTTGGCTTaa                               >  1:715623/1‑251 (MQ=255)                                                                                                                                                                                                                        ctctAACACATGCACCAAGGTCTGGCTTGCCGCGCGAATTTGCGCTTGGTTGCCGTTTGCGAATACCGGCTTGGTGAGTTCCAAATACCAGTCACAGAATTGGTTCCAAGTGAATTCATAAAGCGCGGTGGCACATAAGTCAAAACGGAATTGGCTTAATGCGTTGCGGAAGGTTTCCACAGTGCGGTTGAATTCCGATTGGATCCAACGATCGGCAACGGAAAATTCAATTTCGCCTTGGCTTAAATCCa                          >  1:832241/1‑251 (MQ=255)                                                                                                                                                                                                                            aaCACATGCACCAAGGTCTGGCTTGCCGCGCGAATTTGCGCTTGGTTGCCGTTTGCGAATACCGGCTTGGTGAGTTCCAAATACCAGTCACAGAATTGGTTCCAAGTGAATTCATAAAGCGCGGTGGCACATAAGTCAAAACGGAATTGGCTTAATGCGTTGCGGAAGGTTTCCACAGTGCGGTTGAATTCCGATTGGATCCAACGATCGGCAACGGAAAATTCAATTTCGCCTTGGCTTAAATCCAGCtt                      <  1:211571/251‑1 (MQ=255)                                                                                                                                                                                                                              cacaTGCACCAAGGTCTGGCTTGCCGCGCGAATTTGCGCTTGGTTGCCGTTTGCGAATACCGGCTTGGTGAGTTCCAAATACCAGTCACAGAATTGGTTCCAAGTGAATTCATAAAGCGCGGTGGCACATAAGTCAAAACGGAATTGGCTTAATGc                                                                                                                   >  1:318506/1‑156 (MQ=255)                                                                                                                                                                                                                                caTGCACCAAGGTCTGGCTTGCCGCGCGAATTTGCGCTTGGTTGCCGTTTGCGAATACCGGCTTGGTg                                                                                                                                                                                                         <  1:851203/68‑1 (MQ=255)                                                                                                                                                                                                                                   gCACCAAGGTCTGGCTTGCCGCGCGAATTTGCGCTTGGTTGCCGTTTGCGAATACCGGCTTGGTGAGTTCCAAATACCAGTCACAGAATTGGTTCCAAGTGAATTCATAAAGCGCGGTGGCACATAAGTCAAAACGGAATTGGCTTAATGCGTTGCGGAAGGTTTCCACAGTGCGGTTGAATTCCGATTGGATCCAACGATCGGCAACGGAAAATTCAATTTCGCCTTGGCTTAAATCCAGCTTTTCATTg               >  1:586220/1‑251 (MQ=255)                                                                                                                                                                                                                                   gCACCAAGGTCTGGCTTGCCGCGCGAATTTGCGCTTGGTTGCCGTTTGCGAATACCGGCTTGGTGAGGTCCAAATACCAGTCACAGAATTGGTTACAAGTGAATTCATAAAGCGCGGGGGCACATAAGTCAAAAc                                                                                                                                   >  1:675738/1‑135 (MQ=255)                                                                                                                                                                                                                                    cACCAAGGTCTGGCTTGCCGCGCGAATTTGCGCTTGGTTGCCGTTTGCGAATACCGGCTTGGTGAGTTCCAAATACCAGTCACAGAATTGGTTCCAAGTGAATTCATAAAGCGCGGTGGCACATAAGTCAAAACGGAATTGGCTTAATGCGTTGCGGAAGGTTTCCACAGTGCGGTTGAATTCCGATTGGATCCAACGATCGGCAACGGAAAATTCAATTTCGCCTTGGCTTAAATCCAGCTTTTCATTgg              >  1:379139/1‑251 (MQ=255)                                                                                                                                                                                                                                      ccAAGGTCTGGCTTGCCGCGCGAATTTGCGCTTGGTTGCCGTTTGCGAATACCGGCTTGGTGAGTTCCAAATACCAGTCACAGAATTGGTTCCAAGTGAATTCATAAAGCGCGGTGGCACATAAGTCAAAACGGAATTGGCTTAATGCGTTGCGGAAGGTTTCCACAGTGCGGTTGAATTCCGATTGGATCCAACGATCGGCAACGGAAAATTCAATTTCGCCTTGGCTTAAATCCAGCTTTTCATTGGTa            <  1:715367/251‑1 (MQ=255)                                                                                                                                                                                                                                          ggTCTGGCTTGCCGCGCGAATTTGCGCTTGGTTGCCGTTTGCGAATACCGGCTTGGTGAGTTCCAAATACCAGTCACAGAATTGGTTCCAAGTGAATTCATAAAGCGCGGTGGCACATAAGTCAAAACGGAATTGGCTTAATGCGTTGCGGAAGGTTTCCACAGTGCGGTTGAATTCCGATTGGATCCAACGATCGGCAACGGAAAATTCAATTTCGCCTTGGCTTAAATc                            <  1:662297/231‑1 (MQ=255)                                                                                                                                                                                                                                           gTCTGGCTTGCCGCGCGAATTTGCGCTTGGTTGCCGTTTGCGAATACCGGCTTGGTGAGTTCCAAATACCAGTCACAGAATTGGTTCCAAGTGAATTCATAAAGCGCGGTGGCACATAAGTCAAAACGGAATTGGCTTAATGCGTTGCGGAAGGTTTCcac                                                                                                 <  1:364650/161‑1 (MQ=255)                                                                                                                                                                                                                                           gTCTGGCTTGCCGCGCGAATTTGCGCTTGGTTGCCGTTTGCGAATACCGGCTTGGTGAGTTCCAAATACCAGTCACAGAATTGGTTCCAAGTGAATTCATAAAGCGCGGTGGCACATAAGTCAAAACGGAATTGGCTTAATGCGTTGCGGAAGGTTTCCACAGTGCGGTTGAATTCCGATTGGATCCAACGATCGGCAACGGAAAATTCAATTTCGCCTTGGCTTAAATCCAGCTTTTCATTGGTAAGCa        >  1:325997/1‑250 (MQ=255)                                                                                                                                                                                                                                            tCTGGCTTGCCGCGCGAATTTGCGCTTGGTTGCCGTTTGCGAATACCGGCTTGGTGAGTTCCAAATACCAGTCACAGAATTGGTTCCAAGTGAATTCATAAAGCGCGGTGGCACATAAGTCAAAACGGAATTGGCTTAATGCGTTGCGGAAGGTTTCCACAGTGCGGTTGAATTCCGATTGGATCCAACGATCGGCAACGGAAAATTCAATTTCGCCTTGGCTTAAATCCAGCTTTTCATTGGTAAGCAc       <  1:827842/250‑1 (MQ=255)                                                                                                                                                                                                                                             cTGGCTTGCCGCGCGAATTTGCGCTTGGTTGCCGTTTGCGAATACCGGCTTGGTGAGTTCCAAATACCAGTCACAGAATTGGTTCCAAGTGAATTCATAAAGCGCGGTGGCACATAAGTCAAAACGGAATTGGCTTAATGCGTTGCGGAAGGTTTCCACAGTGCGGTTGAATTCCGATTGGATCCAACGATCGGCAACGGAAAATTCAATTTCGCCTTGGCTTAAATCCAGCTTTTCATTGGTAAGCACGa     >  1:120090/1‑251 (MQ=255)                                                                                                                                                                                                                                                 cTTGCCGCGCGAATTTGCGCTTGGTTGCCGTTTGCGAATACCGGCTTGGTGAGTTCCAAATACCAGTCACAGAATTGGTTCCAAGTGAATTCATAAAGCGCGGTGGCACATAAGTCAAAACGGAATTGGCTTAATGCGTTGCGGAAGGTTTCCACAGTGCGGTTGAATTCCGATTGGATCCAACGATCGGCAACGGAAAATTCAATTTCGCCTTGGCTTAAATCCAGCTTTTCATTGGTAAGCACGAAGc  <  1:503341/250‑1 (MQ=255)                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                      ATGTTGCATTCGGCGCGAATATTACGTACGGCGACGATGACCTCTTTCAACCATGCGATTTCGGCTTCCGCGTCCACATCAAACGCCGTCATGTCCACTTGCGGGAACGGCTGCAACATAATGCTGTCGGCGTCAATGCCGACGAAACCTTTCACTTTCTGCCAGATTTCTTCGGTAATGAATGGAATAACCGGGTGCGCTAAACGCAGTAATTTCTCTAACACATGCACCAAGGTCTGGCTTGTCGCGCGAATTTGCGCTTGGTTGCCGTTTGCGAATACCGGCTTGGTGAGTTCCAAATACCAGTCACAGAATTGGTTCCAAGTGAATTCATAAAGCGCGGTGGCACATAAGTCAAAACGGAATTGGCTTAATGCGTTGCGGAAGGTTTCCACAGTGCGGTTGAATTCCGATTGGATCCAACGATCGGCAACGGAAAATTCAATTTCGCCTTGGCTTAAATCCAGCTTTTCATTGGTAAGCACGAAGC  >  1/1553159‑1553648

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑