Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,551,730	A→G	intergenic (‑107102/‑90034)	unknown ← / → unknown	tRNA‑Met‑CAT/tRNA‑Gly‑GCC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,551,730	0	A	G	100.0%	192.7 / NA	70	intergenic (‑107102/‑90034)	unknown/unknown	tRNA‑Met‑CAT/tRNA‑Gly‑GCC
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (28/42);  total (28/42)

ACTTTATCACGGTGAAAGTCGCTCTGTTCGGCAAATTTATGCGGAGCCGTTAAATGATAAGAAGTGTAAAACGTGTTGAAAAACTACCGCACTTTTCCGTTAGGCATGGAAGAAACCGATGATTTTCTCATTTCGCTTGTCGGCACACAGGAAAAAACGGCTCTACTCTACTATCAAAATCAATGGTAATGTCCCCTACATTCCCCGCTACCAGCCATATTTTTAAATTACCTATCGGTATCGTCGGACAGGAGCAACTTAATCTTTCCCATAATTGCGAGAACGAATGGCTTTGCCTGAAGTTCGGCTAAGACAACCATTGAATAAGTATATCTATAACCTCAATCAGTCCTGCACCGGCTAAAATAACCCCGATTAATCCTAACCTCCAATTACGTTTTTCATTAATACACTGTTCTGCTTTTTGTTGAGTATAACTCAATATTTGGTGGACCTGTGCCAACTGAGAAG  >  1/1551483‑1551953                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                aCTTTATCACGGTGAAAGTCGCTCTGTTCGGCAAATTTATGCGGAGCCGTTAAATGATAAGAAGTGTAAAACGTGTTGAAAAACTACCGCACTTTTCCGTTAGGCATGGAAGAAACCGATGATTTTCTCATTTCGCTTGTCGGCACACAGGAAAAAACGGCTCTACTCTACTATCAAAATCAATGGTAATGTCCCCTACATTCCCCGCTACCAGCCATATTTTTAAATTACCTATCGGTATCGTCGGGCAg                                                                                                                                                                                                                              >  1:791411/1‑251 (MQ=255)               aaaGTCGCTCTGTTCGGCAAATTTATGCGGAGCCGTTAAATGATAAGAAGTGTAAAACGTGTTGAAAAACTACCGCACTTTTCCGTTAGGCATGGAAGAAACCGATGATTTTCTCATTTCGCTTGTCGGCACACAGGAAAAAACGGCTCTACTCTACTATCAAAATCAATGGTAATGTCCCCTACATTCCCCGCTACCAGCCATATTTTTAAATTACCTATCGGTATCGTCGGGCAGGAGCAACTTAGTCt                                                                                                                                                                                                                <  1:841452/251‑1 (MQ=255)                         tGTTCGGCAAATTTATGCGGAGCCGTTAAATGATAAGAAGTGTAAAACGTGTTGAAAAACTACCGCACTTTTCCGTTAGGCATGGAAGAAACCGATGATTTTCTCATTTCGCTTGTCGGCACACAGGAAAAAACGGCTCTACTCTACTATCAAAATCAATGGTAATGTCCCCTACATTCCCCGCTACCAGCCATATTTTTAAATTACCTATCGGTATCGTCGGGCAGGAGCAACTTAATCTTTCCCATAAt                                                                                                                                                                                                      <  1:603237/251‑1 (MQ=255)                           ttCGGCAAATTTATGCGGAGCCGTTAAATGATAAGAAGTGTAAAACGTGTTGAAAAACTACCGCACTTTTCCGTTAGGCATGGAAGAAACCGATGATTTTCTCATTTCGCTTGTCGGCACACAGGAAAAAACGGCTCTACTCTACTATCAAAATCAATGGTAATGTCCCCTACATTCCCCGCTACCAGCCATATTTTTAAATTACCTATCGGTATCGTCGGGCAGGAGCAACTTAATCTTTCCCATAATTg                                                                                                                                                                                                    <  1:585328/251‑1 (MQ=255)                             cGGCAAATTTATGCGGAGCCGTTAAATGATAAGAAGTGTAAAACGTGTTGAAAAACTACCGCACTTTTCCGTTAGGCATGGAAGAAACCGATGATTTTCTCATTTCGCTTGTCGGCACACAGGAAAAAACGGCTCTACTCTACTATCAAAATCAATGGTAATGTCCCCTACATTCCCCGCTACCAGCCATATTTTTAAATTACCTATCGGTATCGTCGGGCAGGAGCAACTTAATCTTTCCCATAATTGCg                                                                                                                                                                                                  >  1:597276/1‑251 (MQ=255)                                    tttATGCGGAGCCGTTAAATGATAAGAAGTGTAAAACGTGTTGAAAAACTACCGCACTTTTCCGTTAGGCATGGAAGAAACCGATGATTTTCTCATTTCGCTTGTCGGCACACAGGAAAAAACGGCTCTACTCTACTATCAAAATCAATGGTAATGTCCCCTACATTCCCCGCTACCAGCCATATTTTTAAATTACCTATCGGTATCGTCGGGCAGGAGCAACTTAATCTTTCCCATAATTGCGAGAACGa                                                                                                                                                                                           >  1:552250/1‑251 (MQ=255)                                      tATGCGGAGCCGTTAAATGATAAGAAGTGTAAAACGTGTTGAAAAACTACCGCACTTTTCCGTTAGGCATGGAAGAAACCGATGATTTTCTCATTTCGCTTGTCGGCACACAGGAAAAAACGGCTCTACTCTACTATCAAAATCAATGGTAATGTCCCCTACATTCCCCGCTACCAGCCATATTTTTAAATTACCTATCGGTATCGTCGGGCAGGAGCAACTTAATCTTTCCCATAATTGCGAGAACGAAt                                                                                                                                                                                         <  1:475696/251‑1 (MQ=255)                                                 gTTAAATGATAAGAAGTGTAAAACGTGTTGAAAAACTACCGCACTTTTCCGTTAGGCATGGAAGAAACCGATGATTTTCTCATTTCGCTTGTCGGCACACAGGAAAAAACGGCTCTACTCTACTATCAAAATCAATGGTAATGTCCCCTACATTCCCCGCTACCAGCCATATTTTTAAATTACCTATCGGTATCGTCGGGCAGGAGCAACTTAATCTTTCCCATAATTg                                                                                                                                                                                                    >  1:480829/1‑229 (MQ=255)                                                 gTTAAATGATAAGAAGTGTAAAACGTGTTGAAAAACTACCGCACTTTTCCGTTAGGCATGGAAGAAACCGATGATTTTCTCATTTCGCTTGTCGGCACACAGGAAAAAACGGCTCTACTCTACTATCAAAATCAATGGTAATGTCCCCTACATTCCCCGCTACCAGCCATATTTTTAAATTACCTATCGGTATCGTCGGGCAGGAGCAACTTAATCTTTCCCATAATTGCGAGAACGAATGGCTTTGc                                                                                                                                                                                 >  1:433094/1‑248 (MQ=255)                                                      aTGATAAGAAGTGTAAAACGTGTTGAAAAACTACCGCACTTTTCCGTTAGGCATGGAAGAAACCGATGATTTTCTCATTTCGCTTGTCGGCACACAGGAAAAAACGGCTCTACTCTACTATCAAAATCAATGGTAATGTCCCCTACATTCCCCGCTACCAGCCATATTTTTAAATTACCTATCGGTATCGTCGGGCAGGAGCAACTTAATCTTTCCCATAATTGCGAGAACGAATGGCTTTGCCTGAAGtt                                                                                                                                                                         >  1:97579/1‑251 (MQ=255)                                                           aagaagTGTAAAACGTGTTGAAAAACTACCGCACTTTTCCGTTAGGCATGGAAGAAACCGATGATTTTCTCATTTCGCTTGTCGGCACACAGGAAAAAACGGCTCTACTCTACTATCAAAATCAATGGTAATGTCCCCTACATTCCCCGCTACCAGCCATATTTTTAAATTACCTATCGGTATCGTCGGGCAGGAGCAACTTAATCTTTCCCATAATTGCGAGAACGAATGGCTTTGCCTGAAGTTCGGCt                                                                                                                                                                    <  1:578053/251‑1 (MQ=255)                                                             gaagTGTAAAACGTGTTGAAAAACTACCGCACTTTTCCGTTAGGCATGGAAGAAACCGATGATTTTCTCATTTCGCTTGTCGGCACACAGGAAAAAACGGCTCTACTCTACTATCAAAATCAATGGTAATGTCCCCTACATTCCCCGCTACCAGCCATATTTTTAAATTACCTATCGGTATCGTCGGGCAGGAGCAACTTAATCTTTCCCATAATTGCGAGAACGAATGGCTTTGCCTGAAGTTCGGCTaa                                                                                                                                                                  >  1:400346/1‑251 (MQ=255)                                                                 tgtAAAACGTGTTGAAAAACTACCGCACTTTTCCGTTAGGCATGGAAGAAACCGATGATTTTCTCATTTCGCTTGTCGGCACACAGGAAAAAACGGCTCTACTCTACTATCAAAATCAATGGTAATGTCCCCTACATTCCCCGCTACCAGCCATATTTTTAAATTACCTATCGGTATCGTCGGGCAGGAGCAACTTAATCTTTCCCATAATTGCGAGAACGAATGGCTTTGCCTGAAGTTCGGCTAAGAc                                                                                                                                                               <  1:476932/250‑1 (MQ=255)                                                                 tgtAAAACGTGTTGAAAAACTACCGCACTTTTCCGTTAGGCATGGAAGAAACCGATGATTTTCTCATTTCGCTTGTCGGCACACAGGAAAAAACGGCTCTACTCTACTATCAAAATCAATGGTAATGTCCCCTACATTCCCCGCTACCAGCCATATTTTTAAATTACCTATCGGTATCGTCGGGCAGGAGCAACTTAATCTTTCCCATAATTGCGAGAACGAATGGCTTTGCCTGAAGTTCGGCTAAGACa                                                                                                                                                              >  1:478291/1‑251 (MQ=255)                                                                            ttGAAAAACTACCGCACTTTTCCGTTAGGCATGGAAGAAACCGATGATTTTCTCATTTCGCTTGTCGGCACACAGGAAAAAACGGCTCTACTCTACTATCAAAATCAATGGTAATGTCCCCTACATTCCCCGCTACCAGCCATATTTTTAAATTACCTATCGGTATCGTCGGGCAGGAGCAACTTAATCTTTCCCATAATTGCGAGAACGAATGGCTTTGCCTGAAGTTCGGCTAAGACAACCATTGAAt                                                                                                                                                    <  1:646902/250‑1 (MQ=255)                                                                            ttGAAAAACTACCGCACTTTTCCGTTAGGCATGGAAGAAACCGATGATTTTCTCATTTCGCTTGTCGGCACACAGGAAAAAACGGCTCTACTCTACTATCAAAATCAATGGTAATGTCCCCTACATTCCCCGCTACCAGCCATATTTTTAAATTACCTATCGGTATCGTCGGGCAGGAGCAACTTAATCTTTCCCATAATTGCGAGAACGAATGGCTTTGCCTGAAGTTCGGCTAAGACAACCATTGAAt                                                                                                                                                    <  1:514116/250‑1 (MQ=255)                                                                            ttGAAAAACTACCGCACTTTTCCGTTAGGCATGGAAGAAACCGATGATTTTCTCATTTCGCTTGTCGGCACACAGGAAAAAACGGCTCTACTCTACTATCAAAATCAATGGTAATGTCCCCTACATTCCCCGCTACCAGCCATATTTTTAAATTACCTATCGGTATCGTCGGGCAGGAGCAACTTAATCTTTCCCATAATTGCGAGAACGAATGGCTTTGCCTGAAGTTCGGCTAAGACAACCATTGAAt                                                                                                                                                    <  1:45473/250‑1 (MQ=255)                                                                                    cTACCGCACTTTTCCGTTAGGCATGGAAGAAACCGATGATTTTCTCATTTCGCTTGTCGGCACACAGGAAAAAACGGCTCTACTCTACTATCAAAATCAATGGTAATGTCCCCTACATTCCCCGCTACCAGCCATATTTTTAAATTACCTATCGGTATCGTCGGGCAGGAGCAACtt                                                                                                                                                                                                                     <  1:558886/177‑1 (MQ=255)                                                                                    cTACCGCACTTTTCCGTTAGGCATGGAAGAAACCGATGATTTTCTCATTTCGCTTGTCGGCACACAGGAAAAAACGGCTCTACTCTACTATCAAAATCAATGGTAATGTCCCCTACATTCCCCGCTACCAGCCATATTTTTAAATTACCTATCGGTATCGTCGGGCAGGAGCAACTTAATCTTTCCCATAATTGCGAGAACGAATGGCTTTGCCTGAAGTTCGGCTAAGACAACCATTGAATAAGTatat                                                                                                                                            >  1:534117/1‑250 (MQ=255)                                                                                    cTACCGCACTTTTCCGTTAGGCATGGAAGAAACCGATGATTTTCTCATTTCGCTTGTCGGCACACAGGAAAAAACGGCTCTACTCTACTATCAAAATCAATGGTAATGTCCCCTACATTCCCCGCTACCAGCCATATTTTTAAATTACCTATCGGTATCGTCGGGCAGGAGCAACTTAATCTTTCCCATAATTGCGAGAACGAATGGCTTTGCCTGAAGTTCGGCTAAGACAACCATTGAATAAGTATATc                                                                                                                                           <  1:83741/251‑1 (MQ=255)                                                                                      aCCGCACTTTTCCGTTAGGCATGGAAGAAACCGATGATTTTCTCATTTCGCTTGTCGGCACACAGGAAAAAACGGCTCTACTCTACTATCAAAATCAATGGTAATGTCCCCTACATTCCCCGCTACCAGCCATATTTTTAAATTACCTATCGGTATCGTCGGGCAGGAGCAACTTAATCTTTCCCATAATTGCGAGAACGAATGGCTTTGCCTGAAGTTCGGCTAAGACAACCATTGAATAAGTATATCta                                                                                                                                         <  1:335517/251‑1 (MQ=255)                                                                                            cTTTTCCGTTAGGCATGGAAGAAACCGATGATTTTCTCATTTCGCTTGTCGGCACACAGGAAAAAACGGCTCTACTCTACTATCAAAATCAATGGTAATGTCCCCTACATTCCCCGCTACCAGCCATATTTTTAAATTACCTATCGGTATCGTCGGGCAGGAGCAACTTAATCTTTCCCATAATTGCGAGAACGAATGGCTTTGCCTGAAGTTCGGCTAAGACAACCATTGAATAAGta                                                                                                                                               <  1:81540/239‑1 (MQ=255)                                                                                              tttCCGTTAGGCATGGAAGAAACCGATGATTTTCTCATTTCGCTTGTCGGCACACAGGAAAAAACGGCTCTACTCTACTATCAAAATCAATGGTAATGTCCCCTACATTCCCCGCTACCAGCCATATTTTTAAATTACCTATCGGTATCGTCGGGCAGGAGCAACTTAATCTTTCCCATAATTGCGAGAACGAATGGCTTTGCCTGAAGTTCGGCTAAGACAACCATTGAATAAGTATATCTATAACCTCa                                                                                                                                 >  1:436684/1‑251 (MQ=255)                                                                                                 ccGTTAGGCATGGAAGAAACCGATGATTTTCTCATTTCGCTTGTCGGCACACAGGAAAAAACGGCTCTACTCTACTATCAAAATCAATGGTAATGTCCCCTACATTCCCCGCTACCAGCCATATTTTTAAATTACCTATCGGTATCGTCGGGCAGGAGCAACTTAATCTTTCCCATAATTGCGAGAACGAATGGCTTTGCCTGAAGTTCGGCTAAGACAACCATTGAATAAGTATATCTATAACCTCAATc                                                                                                                              <  1:493693/251‑1 (MQ=255)                                                                                                       ggCATGGAAGAAACCGATGATTTTCTCATTTCGCTTGTCGGCACACAGGAAAAAACGGCTCTACTCTACTATCAAAATCAATGGTAATGTCCCCTACATTCCCCGCTACCAGCCATATTTTTAAATTACCTATCGGTATCGTCGGGCAGGAGCAACTTAATCTTTCCCATAATTGCGAGAACGAATGGCTTTGCCTGAAGTTCGGCTAAGACAACCATTGAATAAGTATATCtat                                                                                                                                        >  1:207480/1‑235 (MQ=255)                                                                                                           tGGAAGAAACCGATGATTTTCTCATTTCGCTTGTCGGCACACAGGAAAAAACGGCTCTGCGCTACTATCAAAGTCAATGGTAATGTCCCCTACATTCCCCGCTACCAACCATATTTTAAAATTACCTATCGGTATCGTCGGGCAGGAGCAACTTAATCTTTCCCATAATTGCGAGAACGAATGGCTTTGCCTGAAGTTCGGCTAAGACAACCAGTGAATAAGTATATCTATAACCTCAATCAGTCCTGCAc                                                                                                                    <  1:620402/251‑1 (MQ=255)                                                                                                           tGGAAGAAACCGATGATTTTCTCATTTCGCTTGTCGGCACACAGGAAAAAACGGCTCTACTCTACTATCAAAATCAATGGTAATGTCCCCTACATTCCCCGCTACCAGCCATATTTTTAAATTACCTATCGGTATCGTCGGGCAGGAGCAACTTAATCTTTCCCATAATTGCGAGAACGAATGGCTTTGCCTGAAGTTCGGCTAAGACAACCATTGAATAAGTATATCTATAACCTCAATCAGTCCTGCAc                                                                                                                    <  1:138385/251‑1 (MQ=255)                                                                                                           tGGAAGAAACCGATGATTTTCTCATTTCGCTTGTCGGCACACAGGAAAAAACGGCTCTACTCTACTATCAAAATCAATGGTAATGTCCCCTACATTCCCCGCTACCAGCCATATTTTTAAATTACCTATCGGTATCGTCGGGCAGGAGCAACTTAATCTTTCCCATAATTGCGAGAACGAATGGCTTTGCCTGAAGTTCGGCTAAGACAACCATTGAATAAGTATATCTATAACCTCAATCAGTCCTGCAc                                                                                                                    <  1:254215/251‑1 (MQ=255)                                                                                                                    ccGATGATTTTCTCATTTCGCTTGTCGGCACACAGGAAAAAACGGCTCTACTCTACTATCAAAATCAATGGTAATGTCCCCTACATTCCCCGCTACCAGCCATATTTTTAAATTACCTATCGGTATCGTCGGGCAGGAGCAACTTAATCTTTCCCATAATTGCGAGAACGAATGGCTTTGCCTGAAGTTCGGCTAAGACAACCATTGAATAAGTATATCTATAACCTCAATCAGTCCTGCACCGGCTaaaa                                                                                                           >  1:469011/1‑251 (MQ=255)                                                                                                                                 cATTTCGCTTGTCGGCACACAGGAAAAAACGGCTCTACTCTACTATCAAAATCAATGGTAATGTCCCCTACATTCCCCGCTACCAGCCATATTTTTAAATTACCTATCGGTATCGTCGGGCAGGAGCAACTTAATCTTTCCCATAATTGCGAGAACGAATGGCTTTGCCTGAAGTTCGGCTAAGACa                                                                                                                                                              >  1:26430/1‑187 (MQ=255)                                                                                                                                 cATTTCGCTTGTCGGCACACAGGAAAAAACGGCTCTACTCTACTATCAAAATCAATGGTAATGTCCCCTACATTCCCCGCTACCAGCCATATTTTTAAATTACCTATCGGTATCGTCGGGCAGGAGCAACTTAATCTTTCCCATAATTGCGAGAACGAATGGCTTTGCCTGAAGTTCGGCTAAGACa                                                                                                                                                              >  1:150421/1‑187 (MQ=255)                                                                                                                                    ttCGCTTGTCGGCACACAGGAAAAAACGGCTCTACTCTACTATCAAAATCAATGGTAATGTCCCCTACATTCCCCGCTACCAGCCATATTTTTAAATTACCTATCGGTATCGTCGGGCAGGAGCAACTTAATCTTTCCCATAATTGCGAGAACGAATGGCTTTGCCTGAAGTTCGGCTAAGACAACCATTGAATAAGTATATCTATAACCTCAATCAGTCCTGCACCGGCTAAAATAACCCCGATTAATcc                                                                                           <  1:69572/251‑1 (MQ=255)                                                                                                                                      cGCTTGTCGGCACACAGGAAAAAACGGCTCTACTCTACTATCAAAATCAATGGTAATGTCCCCTACATTCCCCGCTACCAGCCATATTTTTAAATTACCTATCGGTATCGTCGGGCAGGAGCAACTTAATCTTTCCCATAATTGCGAGAACGAATGGCTTTGCCTGAAGTTCGGCTAAGACAACCATTGAATAAGTATATCTATAACCTCAATCAGTCCTGCACCGGCTAAAATAACCCCGATTAATCCTa                                                                                         >  1:338710/1‑251 (MQ=255)                                                                                                                                          tGTCGGCACACAGGAAAAAACGGCTCTACTCTACTATCAAAATCAATGGTAATGTCCCCTACATTCCCCGCTACCAGCCATATTTTTAAATTACCTATCGGTATCGTCGGGCAGGAGCAACTTAATCTTTCCCATAATTGCGAGAACGAATGGCTTTGCCTGAAGTTCGGCTAAGACAACCATTGAATAAGTATATCTATAACCTCAATCAGTCCTGCACCGGCTAAAATAACCCCGATTAATCCTAAc                                                                                       >  1:527071/1‑249 (MQ=255)                                                                                                                                           gTCGGCACACAGGAAAAAACGGCTCTACTCTACTATCAAAATCAATGGTAATGTCCCCTACATTCCCCGCTACCAGCCATATTTTTAAATTACCTATCGGTATCGTCGGGCAGGAGCAACTTAATCTTTCCCATAATTGCGAGAACGAATGGCTTTGCCTGAAGTTCGGCTAAGACAACCATTGAATAAGTATATCTATAACCTCAATCAGTCCTGCAc                                                                                                                    >  1:404773/1‑219 (MQ=255)                                                                                                                                            tCGGCACACAGGAAAAAACGGCTCTACTCTACTATCAAAATCAATGGTAATGTCCCCTACATTCCCCGCTACCAGCCATATTTTTAAATTACCTATCGGTATCGTCGGGCAGGAGCAACTTAATCTTTCCCATAATTGCGAGAACGAATGGCTTTGCCTGAAGTTCGGCTAAGACAACCATTGAATAAGTATATCTATAACCTCAATCAGTCCTGCACCGGCTAAAATAACCCCGATTAATCCTAACCTcc                                                                                   <  1:870520/251‑1 (MQ=255)                                                                                                                                                 acacaGGAAAAAACGGCTCTACTCTACTATCAAAATCAATGGTAATGTCCCCTACATTCCCCGCTACCAGCCATATTTTTAAATTACCTATCGGTATCGTCGGGCAGGAGCAACTTAATCTTTCCCATAATTGCGAGAACGAATGGCTTTGCCTGAAGTTCGGCTAAGACAACCATTGAATAAGTATATCTATAACCTCAATCAGTCCTGCACCGGCTAAAATAACCCCGATTAATCCTAACCTCCAATTa                                                                              <  1:763007/251‑1 (MQ=255)                                                                                                                                                      ggAAAAAACGGCTCTACTCTACTATCAAAATCAATGGTAATGTCCCCTACATTCCCCGCTACCAGCCATATTTTTAAATTACCTATCGGTATCGTCGGGCAGGAGCAACTTAATCTTTCCCATAATTGCGAGAACGAATGGCTTTGCCTGAAGTTCGGCTAAGACAACCATTGAATAAGTATATCtat                                                                                                                                        <  1:681244/188‑1 (MQ=255)                                                                                                                                                      ggAAAAAACGGCTCTACTCTACTATCAAAATCAATGGTAATGTCCCCTACATTCCCCGCTACCAGCCATATTTTTAAATTACCTATCGGTATCGTCGGGCAGGAGCAACTTAATCTTTCCCATAATTGCGAGAACGAATGGCTTTGCCTGAAGTTCGGCTAAGACAACCATTGAATAAGTATATCTATAACCTCAATCAGTCCTGCAc                                                                                                                    >  1:486825/1‑208 (MQ=255)                                                                                                                                                      ggAAAAAACGGCTCTACTCTACTATCAAAATCAATGGTAATGTCCCCTACATTCCCCGCTACCAGCCATATTTTTAAATTACCTATCGGTATCGTCGGGCAGGAGCAACTTAATCTTTCCCATAATTGCGAGAACGAATGGCTTTGCCTGAAGTTCGGCTAAGACAACCATTGAATAAGTATATCTATAACCTCAATCAGTCCTGCAc                                                                                                                    <  1:527379/208‑1 (MQ=255)                                                                                                                                                      ggAAAAAACGGCTCTACTCTACTATCAAAATCAATGGTAATGTCCCCTACATTCCCCGCTACCAGCCATATTTTTAAATTACCTATCGGTATCGTCGGGCAGGAGCAACTTAATCTTTCCCATAATTGCGAGAACGAATGGCTTTGCCTGAAGTTCGGCTAAGACAACCATTGAATAAGTATATCTATAACCTCAATCAGTCCTGCACCGGCTAAAATAACCCCGATTAATcc                                                                                           <  1:155903/233‑1 (MQ=255)                                                                                                                                                                 ctctactctaCTATCAAAATCAATGGTAATGTCCCCTACATTCCCCGCTACCAGCCATATTTTTAAATTACCTATCGGTATCGTCGGGCAGGAGCAACTTAATCTTTCCCATAATTGCGAGAACGAATGGCTTTGCCTGAAGTTCGGCTAAGACAACCATTGAATAAGTATATCTATAACCTCAATCAGTCCTGCACCGGCTAAAATAACCCCGATTAATCCTAACCTCCAATTACGTTTTTCATTAATac                                                              <  1:160354/251‑1 (MQ=255)                                                                                                                                                                 ctctactctaCTATCAAAATCAATGGTAATGTCCCCTACATTCCCCGCTACCAGCCATATTTTTAAATTACCTATCGGTATCGTCGGGCAGGAGCAACTTAATCTTTCCCATAATTGCGAGAACGAATGGCTTTGCCTGAAGTTCGGCTAAGACAACCATTGAATAAGTATATCTATAACCTCAATCAGTCCTGCACCGGCTAAAATAACCCCGATTAATCCTAACCTCCAATTACGTTTTTCATTAATac                                                              <  1:624420/251‑1 (MQ=255)                                                                                                                                                                   ctactctactATCAAAATCAATGGTAATGTCCCCTACATTCCCCGCTACCAGCCATATTTTTAAATTACCTATCGGTATCGTCGGGCAGGAGCAACTTAATCTTTCCCATAATTGCGAGAACGAATGGCTTTGCCTGAAGTTCGGCTAAGACAACCATTGAATAAGTATATCTATAAcc                                                                                                                                    <  1:548858/179‑1 (MQ=255)                                                                                                                                                                   ctactctactATCAAAATCAATGGTAATGTCCCCTACATTCCCCGCTACCAGCCATATTTTTAAATTACCTATCGGTATCGTCGGGCAGGAGCAACTTAATCTTTCCCATAATTGCGAGAACGAATGGCTTTGCCTGAAGTTCGGCTAAGACAACCATTGAATAAGTATATCTATAACCTCAATCAGTCCTGCAc                                                                                                                    <  1:302502/195‑1 (MQ=255)                                                                                                                                                                     actctactATCAAAATCAATGGTAATGTCCCCTACATTCCCCGCTACCAGCCATATTTTTAAATTACCTATCGGTATCGTCGGGCAGGAGCAACTTAATCTTTCCCATAATTGCGAGAACGAATGGCTTTGCCTGAAGTTCGGc                                                                                                                                                                     >  1:353890/1‑144 (MQ=255)                                                                                                                                                                        ctactaTCAAAATCAATGGTAATGTCCCCTACATTCCCCGCTACCAGCCATATTTTTAAATTACCTATCGGTATCGTCGGGCAGGAGCAACTTAATCTTTCCCATAATTGCGAGAACGAATGGCTTTGCCTGAAGTTCGGCTAAGACAACCATTGAATAAGTATATCTATAAcc                                                                                                                                    >  1:5620/1‑174 (MQ=255)                                                                                                                                                                          actaTCAAAATCAATGGTAATGTCCCCTACATTCCCCGCTACCAGCCATATTTTTAAATTACCTATCGGTATCGTCGGGCAGGAGCAACTTAATCTTTCCCATAAt                                                                                                                                                                                                      <  1:266527/106‑1 (MQ=255)                                                                                                                                                                             aTCAAAATCAATGGTAATGTCCCCTACATTCCCCGCTACCAGCCATATTTTTAAATTACCTATCGGTATCGTCGGGCAGGAGCAACTTAATCTTTCCCATAATTGCGAGAACGAATGGCTTTGCCTGAAGTTCGGCTAAGACAACCATTGAATAAGTATATCTATAACCTCAATCAGTCCTGCACCGGCTAAAATAACCCCGATTAATCCTAACCTCCAATTACGTTTTTCATTAATACACTGTTCTGCtt                                                  >  1:661914/1‑251 (MQ=255)                                                                                                                                                                                 aaaTCAATGGTAATGTCCCCTACATTCCCCGCTACCAGCCATATTTTTAAATTACCTATCGGTATCGTCGGGCAGGAGCAACTTAATCTTTCCCATAATTGCGAGAACGAATGGCTTTGCCTGAAGTTCGGCTAAGACAACCATTGAATAAGTATATCTATAACCTCAATCAGTCCTGCACCGGCTAAAATAACCCCGATTAATCCTAACCTCCAATTACGTTTTTCATTAATACACTGTTCTGCTTTttg                                              >  1:23143/1‑251 (MQ=255)                                                                                                                                                                                   aTCAATGGTAATGTCCCCTACATTCCCCGCTACCAGCCATATTTTTAAATTACCTATCGGTATCGTCGGGCAGGAGCAACTTAATCTTTCCCATAATTGCGAGAACGAATGGCTTTGCCt                                                                                                                                                                               <  1:228482/120‑1 (MQ=255)                                                                                                                                                                                     cAATGGTAATGTCCCCTACATTCCCCGCTACCAGCCATATTTTTAAATTACCTATCGGTATCGTCGGGCAGGAGCAACTTAATCTTTCCCATAATTGCGAGAACGAATGGCTTTGCCTGAAGTTCGGCTAAGACAACCATTGAATAAGTATATCTATAACCTCAATCAGTCCTGCACCGGCTAAAATAACCCCGATTAATCCTAACCTCCAATTACGTTTTTCATTAATACACTGTTCTGCTTTTTGTTGa                                          >  1:282664/1‑251 (MQ=255)                                                                                                                                                                                                 ccccTACATTCCCCGCTACCAGCCATATTTTTAAATTACCTATCGGTATCGTCGGGCAGGAGCAACTTAATCTTTCCCATAATTGCGAGAACGAATGGCTTTGCCTGAAGTTCGGCTAAGACAACCATTGAATAAGTATATCTATAACCTCAATCAGTCCTGCACCGGCTAAAATAACCCCGATTAATCCTAACCTCCAATTACGTTTTTCATTAATacac                                                            <  1:767390/221‑1 (MQ=255)                                                                                                                                                                                                 ccccTACATTCCCCGCTACCAGCCATATTTTTAAATTACCTATCGGTATCGTCGGGCAGGAGCAACTTAATCTTTCCCATAATTGCGAGAACGAATGGCTTTGCCTGAAGTTCGGCTAAGACAACCATTGAATAAGTATATCTATAACCTCAATCAGTCCTGCACCGGCTAAAATAACCCCGATTAATCCTAACCTCCAATTACGTTTTTCATTAATACACTGTTCTGCTTTttgt                                             <  1:589618/236‑1 (MQ=255)                                                                                                                                                                                                 ccccTACATTCCCCGCTACCAGCCATATTTTTAAATTACCTATCGGTATCGTCGGGCAGGAGCAACCTAATCTTTCCCATAATTGCGAGAACGAATGGCTTTGCCTGAAGTTCGGCTAAGACAACCATTGAATAAGTATATCTATAACCTCAATCAGTCCTGCACCGGCTAAAATAACCCCGATTAATCCTAACCTCCAATTACGTTTTTCATTAATACACTGTTCTGCTTTttgt                                             <  1:430461/236‑1 (MQ=255)                                                                                                                                                                                                  cccTACATTCCCCGCTACCAGCCATATTTTTAAATTACCTATCGGTATCGTCGGGCAGGAGCAACTTAATCTTTCCCATAATTGCGAGAACGAATGGCTTTGCCTGAAGTTCGGCTAAGACAACCATTGAATAAGTATATCTATAACCTCAATCAGTCCTGCACCGGCTAAAATAACCCCGATTAATCCTAACCTCCAATTACGTTTTTCATTAATACACTGTTCTGCTTTttgt                                             >  1:795611/1‑235 (MQ=255)                                                                                                                                                                                                   ccTACATTCCCCGCTACCAGCCATATTTTTAAATTACCTATCGGTATCGTCGGGCAGGAGCAACTTAATCTTTCCCATAATTGCGAGAACGAATGGCTTTGCCTGAAGTTCGGCTAAGACAACCATTGAATAAGTATATCTATAACCTCAATCAGTCCTGCACCGGCTAAAATAACCCCGATTAATCCTAACCTCCAATTACGTTTTTCATTAATACACTGTTCTGCTTTTTGTTGAGTATAACTCAatat                            <  1:179260/251‑1 (MQ=255)                                                                                                                                                                                                   catACATTCCCCGCTACCAGCCATATTTTTAAATTACCTATCGGTATCGTCGGGCAGGAGCAACTTAATCTTTCCCATAATTGCGAGAACGAATGGCTTTGCCTGAAGTTCGGCTAAGACAACCATTGAATAAGTATATCTATAACCTCAATCAGTCCTGCACCGGCTAAAATAACCCCGATTAATCCTAACCTCCAATTACGTTTTTCATTAATACACTGTTCTGCTTTTTGTTGAGTATAACTCAatat                            <  1:294567/249‑1 (MQ=255)                                                                                                                                                                                                    cTACATTCCCCGCTACCAGCCATATTTTTAAATTACCTATCGGTATCGTCGGGCAGGAGCAACTTAATCTTTCCCATAATTGCGAGAACGAATGGCTTTGCCTGAAGTTCGGCTAAGACAACCATTGAAt                                                                                                                                                    >  1:601714/1‑130 (MQ=255)                                                                                                                                                                                                      aCATTCCCCGCTACCAGCCATATTTTTAAATTACCTATCGGTATCGTCGGGCAGGAGCAACTTAATCt                                                                                                                                                                                                                <  1:859281/68‑1 (MQ=255)                                                                                                                                                                                                      aCATTCCCCGCTACCAGCCATATTTTTAAATTACCTATCGGTATCGTCGGGCAGGAGCAACTTAATCTTTCCCATAATTGCGAGAACGAATGGCTTTGCCTGAAGTTCGGCTAAGACAACCATTGAATAAGTATATCTATAACCTCAATCAGTCCTGCAc                                                                                                                    >  1:736110/1‑160 (MQ=255)                                                                                                                                                                                                        aTTCCCCGCTACCAGCCATATTTTTAAATTACCTATCGGTATCGTCGGGCAGGAGCAACTTAATCTTTCCCATAATTGCGAGAACGAATGGCTTTGCCTGAAGTTCGGCTAAGACAACCATTGAATAAGTATATCTATAACCTCAATCAGTCCTGCACCGGCTAAAATAACCCCGATTAATCCTAACCTCCAATTACGTTTTTCATTAATACACTGTTCTGCTTTTTGTTGAGTATAACTCAATATTtggt                       <  1:144853/251‑1 (MQ=255)                                                                                                                                                                                                           ccccGCTACCAGCCATATTTTTAAATTACCTATCGGTATCGTCGGGCAGGAGCAACTTAATCTTTCCCATAATTGCGAGAACGAATGGCTTTGCCTGAAGTTCGGCTAAGACAACCATTGAATAAGTATATCTATAACCTCAATCAGTCCTGCACCGGCTAAAATAACCCCGATTAATCCTAACCTCCAATTACGTTTTTCATTAATACACTGTTCTGCtt                                                  <  1:414770/221‑1 (MQ=255)                                                                                                                                                                                                                        cATATTTTTAAATTACCTATCGGTATCGTCGGGCAGGAGCAACTTAATCTTTCCCATAATTGCGAGAACGAATGGCTTTGCCTGAAGTTCGGCTAAGACAACCATTGAATAAGTATATCTATAACCTCAATCAGTCCTGCACCGGCTAAAATAACCCCGATTAATCCTAACCTCCAATTACGTTTTTCATTAATACACTGTTCTGCTTTTTGTTGAGTATAACTCAATATTTGGTGGACCTGTGCCAACTg       <  1:664088/251‑1 (MQ=255)                                                                                                                                                                                                                            tttttAAATTACCTATCGGTATCGTCGGGCAGGAGCAACTTAATCTTTCCCATAATTGCGAGAACGAATGGCTTTGCCTGAAGTTCGGCTAAGACAACCATTGAATAAGTATATCTATAACCTCAATCAGTCCTGCAc                                                                                                                    <  1:4577/138‑1 (MQ=255)                                                                                                                                                                                                                             ttttAAATTACCTATCGGTATCGTCGGGCAGGAGCAACTTAATCTTTCCCATAATTGCGAGAACGAATGGCTTTGCCTGAAGTTCGGCTAAGACAACCATTGAATAAGTATATCTATAACCTCAATCAGTCCTGCACCGGCTAAAATAACCCCGATTAATCCTAACCTCCAATTACGTTTTTCATTAATACACTGTTCTGCTTTTTGTTGAGTATAACTCAATATTTGGTGGACCTGTGCCAACTGAGAAg  <  1:61015/251‑1 (MQ=255)                                                                                                                                                                                                                                      aCCTATCGGTATCGTCGGGCAGGAGCAACTTAATCTTTCCCATAATTGCGAGAACGAATGGCTTTGCCTGAAGTTCGGCTAAGACAACCATTGAATAAGTATATCTATAACCTCAATCAGTCCTGCACCGGCTAAAATAAccc                                                                                                     >  1:402307/1‑143 (MQ=255)                                                                                                                                                                                                                                        cTATCGGTATCGTCGGGCAGGAGCAACTTAATCTTTCCCATAATTGCGAGAACGAATGGCTTTGCCTGAAGTTCGGCTAAGACAACCATTGAATAAGTATATCTATAACCTCAATCAGTCCTGCACCGGCTAAAATAACCCCGATTAATcc                                                                                           <  1:127252/151‑1 (MQ=255)                                                                                                                                                                                                                                              gTATCGTCGGGCAGGAGCAACTTAATCTTTCCCATAATTGCGAGAACGAATGGCTTTGCCTGAAGTTCGGCTAAGACAACCATTGAATAAGTATATCTATAAcc                                                                                                                                    <  1:543230/104‑1 (MQ=255)                                                                                                                                                                                                                                              gTATCGTCGGGCAGGAGCAACTTAATCTTTCCCATAATTGCGAGAACGAATGGCTTTGCCTGAAGTTCGGCTAAGACAACCATTGAATAAGTATATCTATAACCTCAATCAGTCCTGCACCGGCTAAAATAACCCCGATTAATCCTAACCTCCAATTACGTTTTTCATTAATacac                                                            >  1:145493/1‑176 (MQ=255)                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                ACTTTATCACGGTGAAAGTCGCTCTGTTCGGCAAATTTATGCGGAGCCGTTAAATGATAAGAAGTGTAAAACGTGTTGAAAAACTACCGCACTTTTCCGTTAGGCATGGAAGAAACCGATGATTTTCTCATTTCGCTTGTCGGCACACAGGAAAAAACGGCTCTACTCTACTATCAAAATCAATGGTAATGTCCCCTACATTCCCCGCTACCAGCCATATTTTTAAATTACCTATCGGTATCGTCGGACAGGAGCAACTTAATCTTTCCCATAATTGCGAGAACGAATGGCTTTGCCTGAAGTTCGGCTAAGACAACCATTGAATAAGTATATCTATAACCTCAATCAGTCCTGCACCGGCTAAAATAACCCCGATTAATCCTAACCTCCAATTACGTTTTTCATTAATACACTGTTCTGCTTTTTGTTGAGTATAACTCAATATTTGGTGGACCTGTGCCAACTGAGAAG  >  1/1551483‑1551953

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑