Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,548,942	T→C	intergenic (‑104314/‑92822)	unknown ← / → unknown	tRNA‑Met‑CAT/tRNA‑Gly‑GCC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,548,942	0	T	C	100.0%	164.6 / NA	60	intergenic (‑104314/‑92822)	unknown/unknown	tRNA‑Met‑CAT/tRNA‑Gly‑GCC
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (41/19);  total (41/19)

CCAAAATTGTCAGCCCGGTGGACGGTTATGTGGCACGTCGCAGCACACAAATCGGGCAAAAAGTCGCTGTTGGCAGTACATTAATGGCTGTTATTTCCAACGACAAAATGTGGGTGGATGCCAATTTCAAAGAAACCCAACTGAAAGATATGCGAATCGGACAACCGGTAAAACTGTCTTTTGATTTATATGGTCATGATGTGAAATTCGATGGCAAAGTAGAAGGCATTGAAATGGGCACCGGCAGTGCATTTTCTTTATTGCCTGCACAAAATGCCACCGGCAACTGGATCAAAGTGATACAACGGGTGCCGGTGCGCGTTAGCTTAGACGCGCAACAACTTGCCCGTTACCCCTTGCGAATCGGCTTATCTACCTTGGTTGAAGTGAATATTGCCGACACGAGCGGTGAAATCTTATCGCAGAAGAAACGCACGACACCGCTCTACTCCACCAACACCCTAGATTACGACCAAAGTGCGCTGGAA  >  1/1548695‑1549182                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                 ccAAAATTGTCAGCCCGGTGGACGGTTATGTGGCACGTCGCAGCACACAAATCGGGCAAAAAGTCGCTGTTGGCAGTACATTAATGGCTGTTATTTCCAACGACAAAATGTGGGTGGATGCCAATTTCAAAGAAACCCAACTGAAAGATATGCGAATCGGACAACCGGTAAAACTGTCTTTTGATTTATATGGTCATGATGTGAAATTCGATGGCAAAGTAGAAGGCATTGAAATGGGCACCGGCAGCGCa                                                                                                                                                                                                                                               >  1:876435/1‑251 (MQ=255)       ttGTCAGCCCGGTGGACGGTTATGTGGCACGTCGCAGCACACAAATCGGGCAAAAAGTCGCTGTTGGCAGTACATTAATGGCTGTTATTTCCAACGACAAAATGTGGGTGGATGCCAATTTCAAAGAAACCCAACTGAAAGATATGCGAATCGGACAACCGGTAAAACTGTCTTTTGATTTATATGGTCATGATGTGAAATTCGATGGCAAAGTAGAAGGCATTGAAATGGGCACCGGCAGCGCAtttt                                                                                                                                                                                                                                           >  1:225132/1‑249 (MQ=255)                  gTGGACGGTTATGTGGCACGTCGCAGCACACAAATCGGGCAAAAAGTCGCTGTTGGCAGTACATTAATGGCTGTTATTTCCAACGACAAAATGTGGGTGGATGCCAATTTCAAAGAAACCCAACTGAAAGATATGCGAATCGGACAACCGGTAAAACTGTCTTTTGATTTATATGGTCATGATGTGAAATTCGATGGCAAAGTAGAAGGCATTGAAATGGGCACCGGCAGCGCATTTTCTTTATTGCCTg                                                                                                                                                                                                                               >  1:610753/1‑250 (MQ=255)                         gTTATGTGGCACGTCGCAGCACACAAATCGGGCAAAAAGTCGCTGTTGGCAGTACATTAATGGCTGTTATTTCCAACGACAAAATGTGGGTGGATGCCAATTTCAAAGAAACCCAACTGAAAGATATGCGAATCGGACAACCGGTAAAACTGTCTTTTGATTTATATGGTCATGATGTGAAATTCGATGGCAAAGTAGAAGGCATTGAAATGGGCACCGGCAGCGCATTTTCTTTATTGCCTGCACAAAAt                                                                                                                                                                                                                       >  1:31397/1‑251 (MQ=255)                                 gCACGTCGCAGCACACAAATCGGGCAAAAAGTCGCTGTTGGCAGTACATTAATGGCTGTTATTTCCAACGACAAAATGTGGGTGGATGCCAATTTCAAAGAAACCCAACTGAAAGATATGCGAATCGGACAACCGGTAAAACTGTCTTTTGATTTATATGGTCATGATGTGAAATTCGATGGCAAAGTAGAAGGCATTGAAATGGGCACCGGCAGCGCATTTTCTTTATTGCCTGCACAAAATGCCACCgg                                                                                                                                                                                                               >  1:549869/1‑251 (MQ=255)                                    cGTCGCAGCACACAAATCGGGCAAAAAGTCGCTGTTGGCAGTACATTAATGGCTGTTATTTCCAACGACAAAATGTGGGTGGATGCCAATTTCAAAGAAACCCAACTGAAAGATATGCGAATCGGACAACCGGTAAAACTGTCTTTTGATTTATATGGTCATGATGTGAAATTCGATGGCAAAGTAGAAGGCATTGAAATGGGCACCGGCAGCGCATTTTCTTTATTGCCTGCACAAAATGCCACCGGCaa                                                                                                                                                                                                            >  1:26767/1‑251 (MQ=255)                                             acacaAATCGGGCAAAAAGTCGCTGTTGGCAGTACATTAATGGCTGTTATTTCCAACGACAAAATGTGGGTGGATGCCAATTTCAAAGAAACCCAACTGAAAGATATGCGAATCGGACAACCGGTAAAACTGTCTTTTGATTTATATGGTCATGATGTGAAATTCGATGGCAAAGTAGAAGGCATTGAAATGGGCACCGGCAGCGCATTTTCTTTATTGCCTGCACAAAATGCCACCGGCAACTGGATCaa                                                                                                                                                                                                   >  1:851109/1‑251 (MQ=255)                                                       ggCAAAAAGTCGCTGTTGGCAGTACATTAATGGCTGTTATTTCCAACGACAAAATGTGGGTGGATGCCAATTTCAAAGAAACCCAACTGAAAGATATGCGAATCGGACAACCGGTAAAACTGTCTTTTGATTTATATGGTCATGATGTGAAATTCGATGGCAAAGTAGAAGGCATTGAAATGGGCACCGGCAGCGCATTTTCTTTATTGCCTGCACAAAATGCCACCGGCAACTGGATCAAAGTGATACaa                                                                                                                                                                                         >  1:135379/1‑251 (MQ=255)                                                       ggCAAAAAGTCGCTGTTGGCAGTACATTAATGGCTGTTATTTCCAACGACAAAATGTGGGTGGATGCCAATTTCAAAGAAACCCAACTGAAAGATATGCGAATCGGACAACCGGTAAAACTGTCTTTTGATTTATATGGTCATGATGTGAAATTCGATGGCAAAGTAGAAGGCATTGAAATGGGCACCGGCAGCGCATTTTCTTTATTGCCTGCACAAAATGCCACCGGCAACTGGATCAAAGTGATACaa                                                                                                                                                                                         >  1:364265/1‑251 (MQ=255)                                                                        ggCAGTACATTAATGGCTGTTATTTCCAACGACAAAATGTGGGTGGATGCCAATTTCAAAGAAACCCAACTGAAAGATATGCGAATCGGACAACCGGTAAAACTGTCTTTTGATTTATATGGTCATGATGTGAAATTCGATGGCAAAGTAGAAGGCATTGAAATGGGCACCGGCAGCGCATTTTCTTTATTGCCTGCACAAAATg                                                                                                                                                                                                                      >  1:233924/1‑205 (MQ=255)                                                                         gCAGTACATTAATGGCTGTTATTTCCAACGACAAAATGTGGGTGGATGCCAATTTCAAAGAAACCCAACTGAAAGATATGCGAATCGGACAACCGGTAAAACTGTCTTTTGATTTATATGGTCATGATGTGAAATTCGATGGCAAAGTAGAAGGCATTGAAATGGGCACCGGCAGCGCATTTTCTTTATTGCCTGCACAAAATGCCACCGGCAACTGGATCAAAGTGATACAACGGGTGCCGGTGCGCGtt                                                                                                                                                                       >  1:759427/1‑251 (MQ=255)                                                                            gTACATTAATGGCTGTTATTTCCAACGACAAAATGTGGGTGGATGCCAATTTCAAAGAAACCCAACTGAAAGATATGCGAATCGGACAACCGGTAAAACTGTCTTTTGATTTATATGGTCATGATGTGAAATTCGATGGCAAAGTAGAAGGCATTGAAATGGGCACCGGCAGCGCATTTTCTTTATTGCCTGCACAAAATGCCACCGGCAACTGGATCAAAGTGATACAACGGGTGCCGGTGCGCGTTAg                                                                                                                                                                     >  1:192877/1‑250 (MQ=255)                                                                            gTACATTAATGGCTGTTATTTCCAACGACAAAATGTGGGTGGATGCCAATTTCAAAGAAACCCAACTGAAAGATATGCGAATCGGACAACCGGTAAAACTGTCTTTTGATTTATATGGTCATGATGTGAAATTCGATGGCAAAGTAGAAGGCATTGAAATGGGCACCGGCAGCGCATTTTCTTTATTGCCTGCACAAAATGCCACCGGCAACTGGATCAAAGTGATACAACGGGTGCCGGTGCGCGTTAg                                                                                                                                                                     >  1:5356/1‑250 (MQ=255)                                                                              aCATTAATGGCTGTTATTTCCAACGACAAAATGTGGGTGGATGCCAATTTCAAAGAAACCCAACTGAAAGATATGCGAATCGGACAACCGGTAAAACTGTCTTTTGATTTATATGGTCATGATGTGAAATTCGATGGCAAAGTAGAAGGCATTGAAATGGGCACCGGCAGCGCATTTTCTTTATTGCCTGCACAAAATGCCACCGGCAACTGGATCAAAGTGATACAACGGGTGCCGGTgcccatttc                                                                                                                                                                     >  1:2043/1‑241 (MQ=255)                                                                              aCATTAATGGCTGTTATTTCCAACGACAAAATGTGGGTGGATGCCAATTTCAAAGAAACCCAACTGAAAGATATGCGAATCGGACAACCGGTAAAACTGTCTTTTGATTTATATGGTCATGATGTGAAATTCGATGGCAAAGTAGAAGGCATTGAAATGGGCACCGGCAGCGCATTTTCTTTATTGCCTGCACAAAATGCCACCGGCAACTGGATCAAAGTGATACAACGGGTGCCGGTGCGCGTTAGCtt                                                                                                                                                                  <  1:619507/251‑1 (MQ=255)                                                                              aCATTAATGGCTGTTATTTCCAACGACAAAATGTGGGTGGATGCCAATTTCAAAGAAACCCAACTGAAAGATATGCGAATCGGACAACCGGTAAAACTGTCTTTTGATTTATATGGTCATGATGTGAAATTCGATGGCAAAGTAGAAGGCATTGAAATGGGCACCGGCAGCGCATTTTCTTTATTGCCTGCACAAAATGCCACCGGCAACTGGATCAAAGTGATACAACGGGTGCCGGTGCGCGTTAGCtt                                                                                                                                                                  >  1:538140/1‑251 (MQ=255)                                                                               cATTAATGGCTGTTATTTCCAACGACAAAATGTGGGTGGATGCCAATTTCAAAGAAACCCAACTGAAAGATATGCGAATCGGACAACCGGTAAAACTGTCTTTTGATTTATATGGTCATGATGTGAAATTCGATGGCAAAGTAGAAGGCATTGAAATGGGCACCGGCAGCGCATTTTCTTTATTGCCTGCACAAAATGCCACCGGCAACTGGATCAAAGTGATACAACGGGTGCCGGTGCGCGTTAGCTTa                                                                                                                                                                 >  1:826438/1‑251 (MQ=255)                                                                                aTTAATGGCTGTTATTTCCAACGACAAAATGTGGGTGGATGCCAATTTCAAAGAAACCCAACTGAAAGATATGCGAATCGGACAACCGGTAAAACTGTCTTTTGATTTATATGGTCATGATGTGAAATTCGATGGCAAAGTAGAAGGCATTGAAATGGGCACCGGCAGCGCATTTTCTTTAt                                                                                                                                                                                                                                     >  1:97960/1‑182 (MQ=255)                                                                                aTTAATGGCTGTTATTTCCAACGACAAAATGTGGGTGGATGCCAATTTCAAAGAAACCCAACTGAAAGATATGCGAATCGGACAACCGGTAAAACTGTCTTTTGATTTATATGGTCATGATGTGAAATTCGATGGCAAAGTAGAAGGCATTGAAATGGGCACCGGCAGCGCATTTTCTTTATTGCCTGCACAAAATGCCACCGGCAACTGGATCAAAGTGATACAACGGGTGCCGGTGCGCGTTAGCTTAg                                                                                                                                                                >  1:342702/1‑251 (MQ=255)                                                                                aTTAATGGCTGTTATTTCCAACGACAAAATGTGGGTGGATGCCAATTTCAAAGAAACCCAACTGAAAGATATGCGAATCGGACAACCGGTAAAACTGTCTTTTGATTTATATGGTCATGATGTGAAATTCGATGGCAAAGTAGAAGGCATTGAAATGGGCACCGGCAGCGCATTTTCTTTATTGCCTGCACAAAATGCCACCGGCAACTGGATCAAAGTGATACAACGGGTGCCGGTGCGCGTTAGCTTAg                                                                                                                                                                >  1:260653/1‑251 (MQ=255)                                                                                      ggCTGTTATTTCCAACGACAAAATGTGGGTGGATGCCAATTTCAAAGAAACCCAACTGAAAGATATGCGAATCGGACAACCGGTAAAACTGTCTTTTGATTTATATGGTCATGATGTGAAATTCGATGGCAAAGTAGAAGGCATTGAAATGGGCACCGGCAGCGCATTTTCTTTATTGCCTGCACAAAATGCCACCGGCAACTGGATCAAAGTGATACAACGGGTGCCGGTGCGCGTTAGCTTAGAcgcg                                                                                                                                                           >  1:496053/1‑250 (MQ=255)                                                                                                     cGACAAAATGTGGGTGGATGCCAATTTCAAAGAAACCCAACTGAAAGATATGCGAATCGGACAACCGGTAAAACTGTCTTTTGATTTATATGGTCATGATGTGAAATTCGATGGCAAAGTAGAAGGCATTGAAATGGGCACCGGCAGCGCATTTTCTTTATTGCCTGCACAAAATGCCACCGGCAACTGGATCAAAGTGATACAACGGGTGCCGGTGCGCGTTAGCTTAGACGCGCAACAACTTGCCCGtt                                                                                                                                           >  1:329679/1‑251 (MQ=255)                                                                                                        cAAAATGTGGGTGGATGCCAATTTCAAAGAAACCCAACTGAAAGATATGCGAATCGGACAACCGGTAAAACTGTCTTTTGATTTATATGGTCATGATGTGAAATTCGATGGCAAAGTAGAAGGCATTGAAATGGGCACCGGCAGCGCATTTTCTTTATTGCCTGCACAAAATGCCACCGGCAACTGGATCAAAGTGATACAACGGGTGCCGGTGCGCGTTAGCTTAGACGCGCAACAACTTGCCCGTTAcc                                                                                                                                        <  1:508618/251‑1 (MQ=255)                                                                                                         aaaaTGTGGGTGGATGCCAATTTCAAAGAAACCCAACTGAAAGATATGCGAATCGGACAACCGGTAAAACTGTCTTTTGATTTATATGGTCATGATGTGAAATTCGATGGCAAAGTAGAAGGCATTGAAATGGGCACCGGCAGCGCATTTTCTTTATTGCCTGCACAAAATGCCACCGGCAACTGGATCAAAGTGATACAACGGGTGCCGGTGCGCGTTAGCTTAGACGCGCAACAACTTGCCCGTTAccc                                                                                                                                       <  1:80554/251‑1 (MQ=255)                                                                                                           aaTGTGGGTGGATGCCAATTTCAAAGAAACCCAACTGAAAGATATGCGAATCGGACAACCGGTAAAACTGTCTTTTGATTTATATGGTCATGATGTGAAATTCGATGGCAAAGTAGAAGGCATTGAAATGGGCACCGGCAGCGCATTTTCTTTATTGCCTGCACAAAATGCCACCGGCAACTGGATCAAAGTGATACAACGGGTGCCGGTGCGCGTTAGCTTAGACGCGCAACAACTTGCCCGTTAccc                                                                                                                                       >  1:590487/1‑249 (MQ=255)                                                                                                                       tGCCAATTTCAAAGAAACCCAACTGAAAGATATGCGAATCGGACAACCGGTAAAACTGTCTTTTGATTTATATGGTCATGATGTGAAATTCGATGGCAAAGTAGAAGGCATTGAAATGGGCACCGGCAGCGCATTTTCTTTATTGCCTGCACAAAATGCCACCGGCAACTGGATCAAAGTGATACAACGGGTGCCGGTGCGCGTTAGCTTAGACGCGCAACAACTTGCCCGTTACCCCTTGCGAATCgg                                                                                                                           >  1:116395/1‑249 (MQ=255)                                                                                                                             tttCAAAGAAACCCAACTGAAAGATATGCGAATCGGACAACCGGTAAAACTGTCTTTTGATTTATATGGTCATGATGTGAAATTCGATGGCAAAGTAGAAGGCATTGAAATGGGCACCGGCAGCGCATTTTCTTTATTGCCTGCACAAAATGCCACCGGCAACTGGATCAAAGTGATACAACGGGTGCCGGTGCGCGTTAGCTTAGACGCGCAACAACTTGCCCGTTACCCCTTGCGAATCGGCTTATCTa                                                                                                                   >  1:616349/1‑251 (MQ=255)                                                                                                                               tCAAAGAAACCCAACTGAAAGATATGCGAATCGGACAACCGGTAAAACTGTCTTTTGATTTATATGGTCATGATGTGAAATTCGATGGCAAAGTAGAAGGCATTGAAATGGGCACCGGCAGCGCATTTTCTTTATTGCCTGCACAAAATGCCACCGGCAACTGGATCAAAGTGATACAACGGGTGCCGGTGCGCGTTAGCTTAGACGCGCAACAACTTGCCCGTTACCCCTTGCGAATCGGCTTATCTAcc                                                                                                                 <  1:190798/251‑1 (MQ=255)                                                                                                                                            aCTGAAAGATATGCGAATCGGACAACCGGTAAAACTGTCTTTTGATTTATATGGTCATGATGTGAAATTCGATGGCAAAGTAGAAGGCATTGAAATGGGCACCGGCAGCGCATTTTCTTTATTGCCTGCACAAAATGCCACCGGCAACTGGATCAAAGTGATACAACGGGTGCCGGTGCGCGTTAGCTTAGACGCGCAACAACTTGCCCGTTACCCCTTGCGAATCGGCTTATCTACCTTGGTTGAAGTGa                                                                                                    >  1:87522/1‑251 (MQ=255)                                                                                                                                            aCTGAAAGATATGCGAATCGGACAACCGGTAAAACTGTCTTTTGATTTATATGGTCATGATGTGAAATTCGATGGCAAAGTAGAAGGCATTGAAATGGGCACCGGCAGCGCATTTTCTTTATTGCCTGCACAAAATGCCACCGGCAACTGGATCAAAGTGATACAACGGGTGCCGGTGCGCGTTAGCTTAGACGCGCAACAACTTGCCCGTTACCCCTTGCGAATCGGCTTATCTACCTTGGTTGAAGTGa                                                                                                    >  1:360323/1‑251 (MQ=255)                                                                                                                                               gAAAGATATGCGAATCGGACAACCGGTAAAACTGTCTTTTGATTTATATGGTCATGATGTGAAATTCGATGGCAAAGTAGAAGGCATTGAAATGGGCACCGGCAGCGCATTTTCTTTATTGCCTGCACAAAATGCCACCGGCAACTGGATCAAAGTGATACAACGGGTGCCGGTGCGCGTTAGCTTAGACGCGCAACAACTTGCCCGTTACCCCTTGCGAATCGGCTTATCTACCTTGGTTGAAGTGAata                                                                                                 >  1:407849/1‑251 (MQ=255)                                                                                                                                                aaaGATATGCGAATCGGACAACCGGTAAAACTGTCTTTTGATTTATATGGTCATGATGTGAAATTCGATGGCAAAGTAGAAGGCATTGAAATGGGCACCGGCAGCGCATTTTCTTTATTGCCTGCACAAAATGCCACCGGCAACTGGATCAAAGTGATACAACGGGTGCCGGTGCGCGTTAGCTTAGACGCGCAACAACTTGCCCGTTACCCCTTGCGAATCGGCTTATCTACCTTGGTTGAAGTGAatat                                                                                                <  1:652758/251‑1 (MQ=255)                                                                                                                                                    atatGCGAATCGGACAACCGGTAAAACTGTCTTTTGATTTATATGGTCATGATGTGAAATTCGATGGCAAAGTAGAAGGCATTGAAATGGGCACCGGCAGCGCATTTTCTTTATTGCCTGCACAAAATGCCACCGGCAACTGGATCAAAGTGATACAACGGGTGCCGGTGCGCGTTAGCTTAGACGCGCAACAACTTGCCCGTTACCCCTTGCGAATCGGCTTATCTACCTTGGTTGAAGTGAATATTGc                                                                                             <  1:162342/250‑1 (MQ=255)                                                                                                                                                          gAATCGGACAACCGGTAAAACTGACTTTTGATTTATATGGTCATGATGTGAAATTCGATGGCAAAGTAGACGGCATTGAAATGGGCACCGGCAGCGCATTTTCTTTATTGCCTGCACAAAATGCCACCGGCAACTGGATCAAAg                                                                                                                                                                                                 >  1:347051/1‑144 (MQ=255)                                                                                                                                                           aaTCGGACAACCGGTAAAACTGTCTTTTGATTTATATGGTCATGATGTGAAATTCGATGGCAAAGTAGAAGGCATTGAAATGGGCACCGGCAGCGCATTTTCTTTATTGCCTGCACAAAATGCCACCGGCAACTGGATCAAAGTGATACAACGGGTGCCGGTGCGCGTTAGCTTAGACGCGCAACAACTTGCCCGTTACCCCTTGCGAATCGGCTTATCTACCTTGGTTGAAGTGAATATTGCCGACACg                                                                                      <  1:505244/250‑1 (MQ=255)                                                                                                                                                                     ccGGTAAAACTGTCTTTTGATTTATATGGTCATGATGTGAAATTCGATGGCAAAGTAGAAGGCATTGAAATGGGCACCGGCAGCGCATTTTCTTTATTGCCTGCACAAAATGCCACCGGCAACTGGATCAAAGTGATACAACGGGTGCCGGTGCGCGTTAGCTTAGACGCGCAACAACTTGCCCGTTACCCCTTGCGAATCGGCTTATCTACCTTGGTTGAAGTGAATATTGCCGACACGAGCGGTGAAAt                                                                           >  1:731212/1‑251 (MQ=255)                                                                                                                                                                     ccGGTAAAACTGTCTTTTGATTTATATGGTCATGATGTGAAATTCGATGGCAAAGTAGAAGGCATTGAAATGGGCACCGGCAGCGCATTTTCTTTATTGCCTGCACAAAATGCCACCGGCAACTGGATCAAAGTGATACAACGGGTGCCGGTGCGCGTTAGCTTAGACGCGCAACAACTTGCCCGTTACCCCTTGCGAATCGGCTTATCTACCTTGGTTGAAGTGAATATTGCCGACACGAGCGGTGAAAt                                                                           >  1:225570/1‑251 (MQ=255)                                                                                                                                                                      cGGTAAAACTGTCTTTTGATTTATATGGTCATGATGTGAAATTCGATGGCAAAGTAGAAGGCATTGAAATGGGCACCGGCAGCGCATTTTCTTTATTGCCTGCACAAAATGCCACCGGCAACTGGATCAAAGTGATACAACGGGTGCCGGTGCGCGTTAGCTTAGACGCGCAACAACTTGCCCGTTACCCCTTGCGAATCGGCTTATCTACCTTGGTTGAAGTGAATATTGCCGACACGAGCGGGGAAAt                                                                           >  1:320822/1‑250 (MQ=255)                                                                                                                                                                        gTAAAACTGTCTTTTGATTTATATGGTCATGATGTGAAATTCGATGGCAAAGTAGAAGGCATTGAAATGGGCACCGGCAGCGCATTTTCTTTATTGCCTGCACAAAATGCCACCGGCAACTGGATCAAAGTGATACAACggg                                                                                                                                                                                     <  1:368559/142‑1 (MQ=255)                                                                                                                                                                                        aTTTATATGGTCATGATGTGAAATTCGATGGCAAAGTAGAAGGCATTGAAATGGGCACCGGCAGCGCATTTTCTTTATTGCCTGCACAAAATGCCACCGGCAACTGGATCAAAGTGATACAACGGGTGCCGGTGCGCGTTAGCTTAGACGCGCAACAACTTGCCCGTTACCCCTTGCGAATCGGCTTATCTACCTTGGTTGAAGTGAATATTGCCGACACGAGCGGTGAAATCTTATCGCAGAAGAAACg                                                         >  1:782897/1‑250 (MQ=255)                                                                                                                                                                                        aTTTATATGGTCATGATGTGAAATTCGATGGCAAAGTAGAAGGCATTGAAATGGGCACCGGCAGCGCATTTTCTTTATTGCCTGCACAAAATGCCACCGGCAACTGGATCAAAGTGATACAACGGGTGCCGGTGCGCGTTAGCTTAGACGCGCAACAACTTGCCCGTTACCCCTTGCGAATCGGCTTATCTACCTTGGTTGAAGTGAATATTGCCGACACGAGCGGTGAAATCTTATCGCAGAAGAAACg                                                         >  1:187021/1‑250 (MQ=255)                                                                                                                                                                                            atatGGTCATGATGTGAAATTCGATGGCAAAGTAGAAGGCATTGAAATGGGCACCGGCAGCGCATTTTCTTTATTGCCTGCACAAAATGCCACCGGCAACTGGATCAAAGTGATACAACGGGTGCCGGTGCGCGTTAGCTTAGACGCGCAACAACTTGCCCGTTACCCCTTGCGAATCGGCTTATCTACCTTGGTTGAAGTGAATATTGCCGACACGAGCGGTGAAATCTTATCGCAGAAGAAACGCACGa                                                    >  1:791846/1‑251 (MQ=255)                                                                                                                                                                                               tGGTCATGATGTGAAATTCGATGGCAAAGTAGAAGGCATTGAAATGGGCACCGGCAGCGCATTTTCTTTATTGCCTGCACAAAATGCCACCGGCAACTGGATCAAAGTGATACAACGGGTGCCGGTGCGCGTTAGCTTAGACGCGCAACAACTTGCCCGTTACCCCTTGCGAATCGGCTTATCTACCTTGGTTGAAGTGAATATTGCCGACACGAGCGGTGAAATCTTATCGCAGAAGAAACGCACGacac                                                 <  1:316491/251‑1 (MQ=255)                                                                                                                                                                                               tGGTCATGATGTGAAATTCGATGGCAAAGTAGAAGGCATTGAAATGGGCACCGGCAGCGCATTTTCTTTATTGCCTGCACAAAATGCCACCGGCAACTGGATCAAAGTGATACAACGGGTGCCGGTGCGCGTTAGCTTAGACGCGCAACAACTTGCCCGTTACCCCTTGCGAATCGGCTTATCTACCTTGGTTGAAGTGAATATTGCCGACACGAGCGGTGAAATCTTATCGCAGAAGAAACGCACGacac                                                 <  1:641914/251‑1 (MQ=255)                                                                                                                                                                                                       atgTGAAATTCGATGGCAAAGTAGAAGGCATTGAAATGGGCACCGGCAGCGCATTTTCTTTATTGCCTGCACAAAATGCCACCGGCAACTGGATCAAAGTGATACAACGGGTGCCGGTGCGCGTTAGCTTAGACGCGCAACAACTTGCCCGTTACCCCTTGCGAATCGGCTTATCTACCtt                                                                                                               <  1:263152/181‑1 (MQ=255)                                                                                                                                                                                                         gtgAAATTCGATGGCAAAGTAGAAGGCATTGAAATGGGCACCGGCAGCGCATTTTCTTTATTGCCTGCACAAAATGCCACCGGCAACTGGATCAAAGTGATACAACGGGTGCCGGTGCGCGTTAGCTTAGACGCGCAACAACTTGCCCGTTACCCCTTGCGAATCGGCTTATCTACCTTGGTTGAAGTGAATATTGCCGACACGAGCGGTGAAATCTTATCGCAGAAGAAACGCACGacac                                                 >  1:765099/1‑241 (MQ=255)                                                                                                                                                                                                          tgAAATTCGATGGCAAAGTAGAAGGCATTGAAATGGGCACCGGCAGCGCATTTTCTTTATTGCCTGCACAAAATGCCACCGGCAACTGGATCAAAGTGATACAACGGGTGCCGGTGCGCGTTAGCTTAGACGCGCAACAACTTGCCCGTTACCCCTTGCGAATCGGCTTATCTACCTTGGTTGAAGTGAATATTGCCGACACGAGCGGTGAAATCTTATCGCAGAAGAAACGCACGACACCGCTCTACTcc                                      <  1:675955/251‑1 (MQ=255)                                                                                                                                                                                                          tgAAATTCGATGGCAAAGTAGAAGGCATTGAAATGGGCACCGGCAGCGCATTTTCTTTATTGCCTGCACAAAATGCCACCGGCAACTGGATCAAAGTGATACAACGGGTGCCGGTGCGCGTTAGCTTAGACGCGCAACAACTTGCCCGTTACCCCTTGCGAATCGGCTTATCTACCTTGGTTGAAGTGAATATTGCCGACACGAGCGGTGAAATCTTATCGCAGAAGAAACGCACGACACCGCTCTACTcc                                      <  1:576107/251‑1 (MQ=255)                                                                                                                                                                                                          tgAAATTCGATGGCAAAGTAGAAGGCATTGAAATGGGCACCGGCAGCGCATTTTCTTTATTGCCTGCACAAAATGCCACCGGCAACTGGATCAAAGTGATACAACGGGTGCCGGTGCGCGTTAGCTTAGACGCGCAACAACTTGCCCGTTACCCCTTGCGAATCGGCTTATCTACCTTGGTTGAAGTGAATATTGCCGACACGAGCGGTGAAATCTTATCGCAGAAGAAACGCACGACACCGCTCTACTc                                       <  1:634119/250‑1 (MQ=255)                                                                                                                                                                                                               ttCGATGGCAAAGTAGAAGGCATTGAAATGGGCACCGGCAGCGCATTTTCTTTATTGCCTGCACAAAATGCCACCGGCAACTGGATCAAAGTGATACAACGGGTGCCGGTGCGCGTTAGCTTAGACGCGCAACAACTTGCCCGTTACCCCTTGCGAATCGGCTTATCTACCTTGGTTGAAGTGAATATTGCCGACACGAGCGGTGAAATCTTATCGCAGAAGAAACGCACGACACCGCTCTACTCCACCaa                                 >  1:341612/1‑251 (MQ=255)                                                                                                                                                                                                               ttCGATGGCAAAGTAGAAGGCATTGAAATGGGCACCGGCAGCGCATTTTCTTTATTGCCTGCACAAAATGCCACCGGCAACTGGATCAAAGTGATACAACGGGTGCCGGTGCGCGTTAGCTTAGACGCGCAACAACTTGCCCGTTACCCCTTGCGAATCGGCTTATCTACCTTGGTTGAAGTGAATATTGCCGACACGAGCGGTGAAATCTTATCGCAGAAGAAACGCACGACACCGCTCTACTCCACCaa                                 <  1:176572/251‑1 (MQ=255)                                                                                                                                                                                                               ttCGATGGCAAAGTAGAAGGCATTGAAATGGGCACCGGCAGCGCATTTTCTTTATTGCCTGCACAAAATGCCACCGGCAACTGGATCAAAGTGATACAACGGGTGCCGGTGCGCGTTAGCTTAGACGCGCAACAACTTGCCCGTTACCCCTTGCGAATCGGCTTATCTACCTTGGTTGAAGTGAATATTGCCGACACGAGCGGTGAAATCTTATCGCAGAAGAAACGCACGACACCGCTCTACTCCACCaa                                 <  1:479859/251‑1 (MQ=255)                                                                                                                                                                                                                    tGGCAAAGTAGAAGGCATTGAAATGGGCACCGGCAGCGCATTTTCTTTATTGCCTGCACAAAATGCCACCGGCAACTGGATCAAAGTGATACAACGGGTGCCGGTGCGCGTTAGCTTAGACGCGCAACAACTTGCCCGTTACCCCTTGCGAATCGGCTTATCTACCTTGGTTGAAGTGAATATTGCCGACACGAGCGGTGAAATCTTATCGCAGAAGAAACGCACGACACCGCTCTACTCCACCAACAcc                             >  1:541185/1‑250 (MQ=255)                                                                                                                                                                                                                         aaGTAGAAGGCATTGAAATGGGCACCGGCAGCGCATTTTCTTTATTGCCTGCACAAAATGCCACCGGCAACTGGATCAAAGTGATACAACGGGTGCCGGTGCGCGTTAGCTTAGACGCGCAACAACTTGCCCGTTACCCCTTGCGAATCGGCTTATCTACCTTGGTTGAAGTGAATATTGCCGACACGAGCGGTGAAATCTTATCGCAGAAGAAACGCACGACACCGCTCTACTCCACCAACACCCTAGAt                       >  1:599084/1‑251 (MQ=255)                                                                                                                                                                                                                           gTAGAAGGCATTGAAATGGGCACCGGCAGCGCATTTTCTTTATTGCCTGCACAAAATGCCACCGGCAACTGGATCAAAGTGATACAACGGGTGCCGGTGCGCGTTAGCTTAGACGCGCAACAACTTGCCCGTTACCCCTTGCGAATCGGCTTATCTACCt                                                                                                                <  1:299471/160‑1 (MQ=255)                                                                                                                                                                                                                           gTAGAAGGCATTGAAATGGGCACCGGCAGCGCATTTTCTTTATTGCCTGCACAAAATGCCACCGGCAACTGGATCAAAGTGATACAACGGGTGCCGGTGCGCGTTAGCTTAGACGCGCAACAACTTGCCCGTTACCCCTTGCGAATCGGCTTATCTACCTTGGTTGAAGTGAATATTGCCGACACGAGCGGTGAAATCTTATCGCAGAAGAAACGCACGACACCGCTCTACTCCACCAACACCCTAGAt                       >  1:166136/1‑249 (MQ=255)                                                                                                                                                                                                                           gTAGAAGGCATTGAAATGGGCACCGGCAGCGCATTTTCTTTATTGCCTGCACAAAATGCCACCGGCAACTGGATCAAAGTGATACAACGGGTGCCGGTGCGCGTTAGCTTAGACGCGCAACAACTTGCCCGTTACCCCTTGCGAATCGGCTTATCTACCTTGGTTGAAGTGAATATTGCCGACACGAGCGGTGAAATCTTATCGCAGAAGAAACGCACGACACCGCTCTACTCCACCAACACCCTAGATTa                     >  1:870630/1‑251 (MQ=255)                                                                                                                                                                                                                            tAGAAGGCATTGAAATGGGCACCGGCAGCGCATTTTCTTTATTGCCTGCACAAAATGCCACCGGCAACTGGATCAAAGTGATACAACGGGTGCCGGTGCGCGTTAGCTTAGACGCGCAACAACTTGCCCGTTACCCCTTGCGAATCGGCTTATCTACCTTGGTTGAAGTGAATATTGCCGACACGAGCGGTGAAATCTTATCGCAGAAGAAACGCACGACACCGCTCTACTCCACCAACACCCTAGAtt                      <  1:459074/249‑1 (MQ=255)                                                                                                                                                                                                                                  gCATTGAAATGGGCACCGGCAGCGCATTTTCTTTATTGCCTGCACAAAATGCCACCGGCAACTGGATCAAAGTGATACAACGGGTGCCGGTGCGCGTTAGCTTAGACGCGCAACAACTTGCCCGTTACCCCTTGCGAATCGGCTTATCTACCTTGGTTGAAGTGAATATTGCCGACACGAGCGGTGAAATCTTATCGCAGAAGAAACGCACGACACCGCTCTACTCCACCAACACCCTAGATTACGACCaa              >  1:436857/1‑251 (MQ=255)                                                                                                                                                                                                                                               cACCGGCAGCGCATTTTCTTTATTGCCTGCACAAAATGCCACCGGCAACTGGATCAAAGTGATACAACGGGTGCCGGTGCGCGTTAGCTTAGACGCGCAACAACTTGCCCGTTACCCCTTGCGAATCGGCTTATCTACCTTGGTTGAAGTGAATATTGCCGACACGAGCGGTGAAATCTTATCGCAGAAGAAACGCACGACACCGCTCTACTCCACCAACACCCTAGATTACGACCAAAGTGCGCTGGaa  <  1:24032/250‑1 (MQ=255)                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                 CCAAAATTGTCAGCCCGGTGGACGGTTATGTGGCACGTCGCAGCACACAAATCGGGCAAAAAGTCGCTGTTGGCAGTACATTAATGGCTGTTATTTCCAACGACAAAATGTGGGTGGATGCCAATTTCAAAGAAACCCAACTGAAAGATATGCGAATCGGACAACCGGTAAAACTGTCTTTTGATTTATATGGTCATGATGTGAAATTCGATGGCAAAGTAGAAGGCATTGAAATGGGCACCGGCAGTGCATTTTCTTTATTGCCTGCACAAAATGCCACCGGCAACTGGATCAAAGTGATACAACGGGTGCCGGTGCGCGTTAGCTTAGACGCGCAACAACTTGCCCGTTACCCCTTGCGAATCGGCTTATCTACCTTGGTTGAAGTGAATATTGCCGACACGAGCGGTGAAATCTTATCGCAGAAGAAACGCACGACACCGCTCTACTCCACCAACACCCTAGATTACGACCAAAGTGCGCTGGAA  >  1/1548695‑1549182

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑