Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,547,223	A→G	intergenic (‑102595/‑94541)	unknown ← / → unknown	tRNA‑Met‑CAT/tRNA‑Gly‑GCC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,547,223	0	A	G	100.0%	210.1 / NA	76	intergenic (‑102595/‑94541)	unknown/unknown	tRNA‑Met‑CAT/tRNA‑Gly‑GCC
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (41/35);  total (41/35)

CCGATAAAGTTGCCGAAAAAGTGGAATTTGAAGCAGAAGTACAAGATATTACCCGTGGAGGTTTACGTGTTTTATTGTTGGCAAACGGCGCGTCCATATTTATTCCTGCCTCAACCTTGCACGACAACAAAGAGGAAATTGTTATCAATACCGACGAAATCGCCTTCTATGTCAAAGATCAGCGTCTTTACAAGATCGGAGATATTTTACGCGTGCAACTCACGGAAGTGAAGGAAGCAACACATAGTATTATGGGGATGGTCATCAAGTAAAAAAGTGCGGTGAATCTTAAAGATGTTTTCAAAACGCTCGAAAAAATCACCGCACTTTTGACC‑AAAAAAATCCTGCTTAACGATTTATTTAAGCAGGATTATTTTTTTAGCTAATTAATTAGCTTGCACAAGCTTGACAAGCTGCCTGGAACATCCAAACCAGTTTTTCTTGCTCTTTTATGTAATCACTCATTTG  >  1/1546980‑1547447                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                  ccGATAAAGTTGCCGAAAAAGTGGAATTTGAAGCAGAAGTACAAGATATTACCCGTGGAGGTTTACGTGTTTTATTGTTGGCAAACGGCGCGTCCATATTTATTCCTGCCTCAACCTTGCACGACAACAAAGAGGAAATTGTTATCAATACCGACGAAATCGCCTTCTATGTCAAAGATCAGCGTCTTTACAAGATCGGAGATATTTTACGCGTGCAACTCACGGAAGTGAAGGAAGCAACACGTAGtat                                                                                                                                                                                                                             <  1:54280/250‑1 (MQ=255)  cGATAAAGTTGCCGAAAAAGTGGAATTTGAAGCAGAAGTACAAGATATTACCCGTGGAGGTTTACGTGTTTTATTGTTGGCAAACGGCGCGTCCATATTTATTCCTGCCTCAACCTTGCACGACAACAAAGAGGAAATTGTTATCAATACCGACGAAATCGCCTTCTATGTCAAAGATCAGCGTCTTTACAAGATCGGAGATATTTTACGCGTGCAACTCACGGAAGTGAAGGAAGCAACACGTAGtatta                                                                                                                                                                                                                           <  1:800453/251‑1 (MQ=255)     tAAAGTTGCCGAAAAAGTGGAATTTGAAGCAGAAGTACAAGATATTACCCGTGGAGGTTTACGTGTTTTATTGTTGGCAAACGGCGCGTCCATATTTATTCCTGCCTCAACCTTGCACGACAACAAAGAGGAAATTGTTATCAATACCGACGAAATCGCCTTCTATGTCAAAGATCAGCGTCTTTACAAGATCGGAGATATTTTACGCGTGCAACTCACGGAAGTGAAGGAAGCAACACGTAGTATTATgg                                                                                                                                                                                                                        <  1:467877/251‑1 (MQ=255)     tAAAGTTGCCGAAAAAGTGGAATTTGAAGCAGAAGTACAAGATATTACCCGTGGAGGTTTACGTGTTTTATTGTTGGCAAACGGCGCGTCCATATTTATTCCTGCCTCAACCTTGCACGACAACAAAGAGGAAATTGTTATCAATACCGACGAAATCGCCTTCTATGTCAAAGATCAGCGTCTTTACAAGATCGGAGATATTTTACGCGTGCAACTCACGGAAGTGAAGGAAGCAACACGTAGTATTATgg                                                                                                                                                                                                                        <  1:345695/251‑1 (MQ=255)       aaGTTGCCGAAAAAGTGGAATTTGAAGCAGAAGTACAAGATATTACCCGTGGAGGTTTACGTGTTTTATTGTTGGCAAACGGCGCGTCCATATTTATTCCTGCCTCAACCTTGCACGACAACAAAGAGGAAATTGTTATCAATACCGACGAAATCGCCTTCTATGTCAAAGATCAGCGTCTTTACAAGATCGGAGATATTTTACGCGTGCAACTCACGGAAGTGAAGGAAGCAACACGTAGTATTATgggg                                                                                                                                                                                                                      <  1:829854/251‑1 (MQ=255)       aaGTTGCCGAAAAAGTGGAATTTGAAGCAGAAGTACAAGATATTACCCGTGGAGGTTTACGTGTTTTATTGTTGGCAAACGGCGCGTCCATATTTATTCCTGCCTCAACCATGCACGACAACAAAGAGGAAATTGTTATCAATACCGACGAAATCGCCTTCTATGTCAAAGATCAGCGTCTTTACAAGATCGGAGATATTTTACGCGTGCAACTCACGGAAGTGAAGGAAGCAACACGTAGTATTATgggg                                                                                                                                                                                                                      <  1:274714/251‑1 (MQ=255)             ccGAAAAAGTGGAATTTGAAGCAGAAGTACAAGATATTACCCGTGGAGGTTTACGTGTTTTATTGTTGGCAAACGGCGCGTCCATATTTATTCCTGCCTCAACCTTGCACGACAACAAAGAGGAAATTGTTATCAATACCGACGAAATCGCCTTCTATGTCAAAGATCAGCGTCTTTACAAGATCGGAGATATTTTACGCGTGCAACTCACGGAAGTGAAGGAAGCAACACGTAGTATTATGGGGATGGtc                                                                                                                                                                                                                <  1:775390/251‑1 (MQ=255)                               aaGCAGAAGTACAAGATATTACCCGTGGAGGTTTACGTGTTTTATTGTTGGCAAACGGCGCGTCCATATTTATTCCTGCCTCAACCTTGCACGACAACAAAGAGGAAATTGTTATCAATACCGACGAAATCGCCTTCTATGTCAAAGATCAGCGTCTTTACAAGATCGGAGATATTTTACGCGTGCAACTCACGGAAGTGAAGGAAGCAACACGTAGTATTATgg                                                                                                                                                                                                                        >  1:141470/1‑225 (MQ=255)                                    gAAGTACAAGATATTACCCGTGGAGGTTTACGTGTTTTATTGTTGGCAAACGGCGCGTCCATATTTATTCCTGCCTCAACCTTGCACGACAACAAAGAGGAAATTGTTATCAATACCGACGAAATCGCCTTCTATGTCAAAGATCAGCGTCTTTACAAGATCGGAGATATTTTACGCGTGCAACTCACGGAAGTGAAGGAAGCAACACGTAGTATTATGGGGATGGTCATCAAGTAAAAAAGTGCGGTGaa                                                                                                                                                                                         >  1:75331/1‑251 (MQ=255)                                       gTACAAGATATTACCCGTGGAGGTTTACGTGTTTTATTGTTGGCAAACGGCGCGTCCATATTTATTCCTGCCTCAACCTTGCACGACAACAAAGAGGAAATTGTTATCAATACCGACGAAATCGCCTTCTATGTCAAAGATCAGCGTCTTTACAAGATCGGAGATATTTTACGCGTGCAACTCACGGAAGTGAAGGAAGCAACACGTAGTATTATGGGGa                                                                                                                                                                                                                     <  1:399668/220‑1 (MQ=255)                                       gTACAAGATATTACCCGTGGAGGTTTACGTGTTTTATTGTTGGCAAACGGCGCGTCCATATTTATTCCTGCCTCAACCTTGCACGACAACAAAGAGGAAATTGTTATCAATACCGACGAAATCGCCTTCTATGTCAAAGATCAGCGTCTTTACAAGATCGGAGATATTTTACGCGTGCAACTCACGGAAGTGAAGGAAGCAACACGTAGTATTATGGGGATGGTCATCAAGTAAAAAAGTGCGGTGAAt                                                                                                                                                                                        >  1:22771/1‑249 (MQ=255)                                         aCAAGATATTACCCGTGGAGGTTTACGTGTTTTATTGTTGGCAAACGGCGCGTCCATATTTATTCCTGCCTCAACCTTGCACGACAACAAAGAGGAAATTGTTATCAATACCGACGAAATCGCCTTCTATGTCAAAGATCAGCGTCTTTACAAGATCGGAGATATTTTACGCGTGCAACTCACGGAAGTGAAGGAAGCAACACGTAGTATTATGGGGATGGTCATCAAGTAAAAAAGTGCGGTGAATCTTa                                                                                                                                                                                    >  1:529529/1‑251 (MQ=255)                                            aGATATTACCCGTGGAGGTTTACGTGTTTTATTGTTGGCAAACGGCGCGTCCATATTTATTCCTGCCTCAACCTTGCACGACAACAAAGAGGAAATTGTTATCAATACCGACGAAATCGCCTTCTATGTCAAAGATCAGCGTCTTTACAAGATCGGAGATATTTTACGCGTGCAACTCACGGAAGTGAAGGAAGCAACACGTAGTATTATGGGGATGGTCATCAAGTAAAAAAGTGCGGTGAATCTTAAAg                                                                                                                                                                                 >  1:226044/1‑251 (MQ=255)                                             gATATTACCCGTGGAGGTTTACGTGTTTTATTGTTGGCAAACGGCGCGTCCATATTTATTCCTGCCTCAACCTTGCACGACAACAAAGAGGAAATTGTTATCAATACCGACGAAATCGCCTTCTATGTCAAAGATCAGCGTCTTTACAAGATCGGAGATATTTTACGCGTGCAACTCACGGAAGTGAAGGAAGCAACACGTAGTATTATGGGGATGGTCATCAAGTAAAAAAGTGCGGTGAATCTTaa                                                                                                                                                                                   >  1:402905/1‑248 (MQ=255)                                             gATATTACCCGTGGAGGTTTACGTGTTTTATTGTTGGCAAACGGCGCGTCCATATTTATTCCTGCCTCAACCTTGCACGACAACAAAGAGGAAATTGTTATCAATACCGACGAAATCGCCTTCTATGTCAAAGATCAGCGTCTTTACAAGATCGGAGATATTTTACGCGTGCAACTCACGGAAGTGAAGGAAGCAACACGTAGTATTATGGGGATGGTCATCAAGTAAAAAAGTGCGGTGAATCTTAAAGa                                                                                                                                                                                >  1:491129/1‑251 (MQ=255)                                             gATATTACCCGTGGAGGTTTACGTGTTTTATTGTTGGCAAACGGCGCGTCCATATTTATTCCTGCCTCAACATTGCACGACAACAAAGAGGAAATTGTTATCAATACCGACGAAATCGCCTTCTATGTCAAAGATCAGCGTCTTTACAAGATCGGAGATATTTTACGCGTGCAACTCACGGAAGTGAAGGAAGCAACACGTAGTATTATGGGGATGGTCATCAAGTAAAAAAGTGCGGTGAATCTTAAAGa                                                                                                                                                                                >  1:850249/1‑251 (MQ=255)                                              atatTACCCGTGGAGGTTTACGTGTTTTATTGTTGGCAAACGGCGCGTCCATATTTATTCCTGCCTCAACCTTGCACGACAACAAAGAGGAAATTGTTATCAATACCGACGAAATCGCCTTCTATGTCAAAGATCAGCGTCTTTACAAGATCGGAGATATTTTACGCGTGCAACTCACGGAAGTGAAGGAAGCAACACGTAGTATTATGGGGATGGTCATCAAg                                                                                                                                                                                                          >  1:732616/1‑224 (MQ=255)                                              atatTACCCGTGGAGGTTTACGTGTTTTATTGTTGGCAAACGGCGCGTCCATATTTATTCCTGCCTCAACCTTGCACGACAACAAAGAGGAAATTGTTATCAATACCGACGAAATCGCCTTCTATGTCAAAGATCAGCGTCTTTACAAGATCGGAGATATTTTACGCGTGCAACTCACGGAAGTGAAGGAAGCAACACGTAGTATTATGGGGATGGTCATCAAGTAAAAAAGTGCGGTGAATCTTAAAGAt                                                                                                                                                                               >  1:893958/1‑251 (MQ=255)                                              atatTACCCGTGGAGGTTTACGTGTTTTATTGTTGGCAAACGGCGCGTCCATATTTATTCCTGCCTCAACCTTGCACGACAACAAAGAGGAAATTGTTATCAATACCGACGAAATCGCCTTCTATGTCAAAGATCAGCGTCTTTACAAGATCGGAGATATTTTACGCGTGCAACACACGGAAGTGAAGGAAGCAACACGTAGTATTATGGGGATGGTCATCAAGTAAAAAAGTGCGGTGAATCTTAAAg                                                                                                                                                                                 <  1:144879/249‑1 (MQ=255)                                                    cccGTGGAGGTTTACGTGTTTTATTGTTGGCAAACGGCGCGTCCATATTTATTCCTGCCTCAACCTTGCACGACAACAAAGAGGAAATTGTTATCAATACCGACGAAATCGCCTTCTATGTCAAAGATCAGCGTCTTTACAAGATCGGAGATATTTTACGCGTGCAACTCACGGAAGTGAAGGAAGCAACACGTAGTATTATGGGGATGGTCATCAAGTAAAAAAGTGCGGTGAATCTTAAAGATGTTTTc                                                                                                                                                                         <  1:321552/251‑1 (MQ=255)                                                     ccGTGGAGGTTTACGTGTTTTATTGTTGGCAAACGGCGCGTCCATATTTATTCCTGCCTCAACCTTGCACGACAACAAAGAGGAAATTGTTATCAATACCGACGAAATCGCCTTCTATGTCAAAGATCAGCGTCTTTACAAGATCGGAGATATTTTACGCGTGCAACTCACGGAAGTGAAGGAAGCAACACGTAGTATTATGGGGATGGTCATCAAGTAAAAAAGTGCGGTGAATc                                                                                                                                                                                       <  1:89717/236‑1 (MQ=255)                                                                                           cgTCCATATTTATTCCTGCCTCAACCTTGCACGACAACAAAGAGGAAATTGTTATCAATACCGACGAAATCGCCTTCTATGTCAAAGATCAGCGTCTTTACAAGATCGGAGATATTTTACGCGTGCAACTCACGGAAGTGAAGGAAGCAACACGTAGTATTATgg                                                                                                                                                                                                                        <  1:854460/165‑1 (MQ=255)                                                                                             tCCATATTTATTCCTGCCTCAACCTTGCACGACAACAAAGAGGAAATTGTTATCAATACCGACGAAATCGCCTTCTATGTCAAAGATCAGCGTCTTTACAAGATCGGAGATATTTTACGCGTGCAACTCACGGAAGTGAAGGAAGCAACACGTAGTATTATGGGGATGGTCATCAAGTAAAAAAGTGCGGTGAATCTTAAAGATGTTTTCAAAACGCTCGAAAAAATCACCGCACTTTTGACC‑aaaaaaa                                                                                                                                <  1:84856/250‑1 (MQ=255)                                                                                             tCCATATTTATTCCTGCCTCAACCTTGCACGACAACAAAGAGGAAATTGTTATCAATACCGACGAAATCGCCTTCTATGTCAAAGATCAGCGTCTTTACAAGATCGGAGATATTTTACGCGTGCAACTCACGGAAGTGAAGGAAGCAACACGTAGTATTATGGGGATGGTCATCAAGTAAAAAAGTGCGGTGAATCTTAAAGATGTTTTCAAAACGCTCGAAAAAATCACCGCACTTTTGACC‑aaaaaaa                                                                                                                                <  1:800459/250‑1 (MQ=255)                                                                                               cATATTTATTCCTGCCTCAACCTTGCACGACAACAAAGAGGAAATTGTTATCAATACCGACGAAATCGCCTTCTATGTCAAAGATCAGCGTCTTTACAAGATCGGAGATATTTTACGCGTGCAACTCACGGAAGTGAAGGAAGCAACACGTAGTATTATGGGGATGGTCATCAAGTAAAAAAGTGCGGTGAATCTTAAAGATg                                                                                                                                                                              <  1:478355/203‑1 (MQ=255)                                                                                                atatTTATTCCTGCCTCAACCTTGCACGACAACAAAGAGGAAATTGTTATCAATACCGACGAAATCGCCTTCTATGTCAAAGATCAGCGTCTTTACAAGATCGGAGATATTTTACGCGTGCAACTCACGGAAGTGAAGGAAGCAACACGTAGTATTATGGGGATGGTCATCAAGTAAAAAAg                                                                                                                                                                                                  <  1:287995/182‑1 (MQ=255)                                                                                                 tatttattCCTGCCTCAACCTTGCACGACAACAAAGAGGAAATTGTTATCAATACCGACGAAATCGCCTTCTATGTCAAAGATCAGCGTCTTTACAAGATCGGAGATATTTTACGCGTGCAACTCACGGAAGTGAAGGAAGCAACACGTAGTATTATGGGGATGGTCATCAAGTAAAAAAGTGCGGTGAATCTTAAAGATGTTTTCAAAACGCTCGAAAAAATCACCGCACTTTTGACC‑AAAAAAATc                                                                                                                              >  1:353583/1‑248 (MQ=255)                                                                                                 tatttattCCTGCCTCAACCTTGCACGACAACAAAGAGGAAATTGTTATCAATACCGACGAAATCGCCTTCTATGTCAAAGATCAGCGTCTTTACAAGATCGGAGATATTTTACGCGTGCAACTCACGGAAGTGAAGGAAGCAACACGTAGTATTATGGGGATGGTCATCAAGTAAAAAAGTGCGGTGAATCTTAAAGATGTTTTCAAAACGCTCGAAAAAATCACCGCACTTTTGACC‑AAAAAAATc                                                                                                                              >  1:591446/1‑248 (MQ=255)                                                                                                         ccTGCCTCAACCTTGCACGACAACAAAGAGGAAATTGTTATCAATACCGACGAAATCGCCTTCTATGTCAAAGATCAGCGTCTTTACAAGATCGGAGATATTTTACGCGTGCAACTCACGGAAGTGAAGGAAGCAACACGTAGTATTATGGGGATGGTCATCAAGTAAAAAAGTGCGGTGAATCTTAAAGATGTTTTCAAAACGCTCGAAAAAATCACCGCACTTTTGACC‑AAAAAAATCCTGCTTAACGa                                                                                                                   <  1:296965/251‑1 (MQ=255)                                                                                                           tGCCTCAACCTTGCACGACAACAAAGAGGAAATTGTTATCAATACCGACGAAATCGCCTTCTATGTCAAAGATCAGCGTCTTTACAAGATCGGAGATATTTTACGCGTGCAACTCACGGAAGTGAAGGAAGCAACACGTAGTATTATGGGGATGGTCATCAAGTAAAAAAg                                                                                                                                                                                                  >  1:701555/1‑171 (MQ=255)                                                                                                           tGCCTCAACCTTGCACGACAACAAAGAGGAAATTGTTATCAATACCGACGAAATCGCCTTCTATGTCAAAGATCAGCGTCTTTACAAGATCGGAGATATTTTACGCGTGCAACTCACGGAAGTGAAGGAAGCAACACGTAGTATTATGGGGATGGTCATCAAGTAAAAAAGTGCGGTGAATCTTAAAGATGTTTTCAAAACGCTCGAAAAAATCACCGCACTTTTGACC‑AAAAAAATCCTGCTTAACGatt                                                                                                                 >  1:94318/1‑251 (MQ=255)                                                                                                            gCCTCAACCTTGCACGACAACAAAGAGGAAATTGTTATCAATACCGACGAAATCGCCTTCTATGTCAAAGATCAGCGTCTTTACAAGATCGGAGATATTTTACGCGTGCAACTCACGGAAGTGAAGGAAGCAACACGTAGTATTATGGGGATGGTCATCAAGTAAAAAAATGCGGTGAATCTTAAAGATGTTTTCAAAACGCTCGAAAAAATCACCGCACTTTTGACC‑AAAAAAATCCTGCTTAACGattt                                                                                                                >  1:436483/1‑251 (MQ=255)                                                                                                                  aCCTTGCACGACAACAAAGAGGAAATTGTTATCAATACCGACGAAATCGCCTTCTATGTCAAAGATCAGCGTCTTTACAAGATCGGAGATATTTTACGCGTGCAACTCACGGAAGTGAAGGAAGCAACACGTAGTATTATGGGGATGGTCATCAAGTAAAAAAGTGCGGTGAATCTTAAAGATGTTTTCAAAACGCTCGAAAAAATCACCGCACTTTTGACC‑AAAAAAATCCTGCTTAACGATTTATTTaa                                                                                                          >  1:459497/1‑251 (MQ=255)                                                                                                                    cTTGCACGACAACAAAGAGGAAATTGTTATCAATACCGACGAAATCGCCTTCTATGTCAAAGATCAGCGTCTTTACAAGATCGGAGATATTTTACGCGTGCAACTCACGGAAGTGAAGGAAGCAACACGTAGTATTATGGGGATGGTCATCAAGTAAAAAAGTGCGGTGAATCTTAAAGATGTTTTCAAAACGCTCGAAAAAATCACCGCACTTTTGACC‑AAAAAAATCCTGCTTAACGATTTATTTAAGc                                                                                                        <  1:401851/251‑1 (MQ=255)                                                                                                                    cTTGCACGACAACAAAGAGGAAATTGTTATCAATACCGACGAAATCGCCTTCTATGTCAAAGATCAGCGTCTTTACAAGATCGGAGATATTTTACGCGTGCAACTCACGGAAGTGAAGGAAGCAACACGTAGTATTATGGGGATGGTCATCAAGTAAAAAAGTGCGGTGAATCTTAAAGATGTTTTCAAAACGCTCGAAAAAATCACCGCACTTTTGACC‑AAAAAAATCCTGCTTAACGATTTATTTAAGc                                                                                                        <  1:292572/251‑1 (MQ=255)                                                                                                                          cGACAACAAAGAGGAAATTGTTATCAATACCGACGAAATCGCCTTCTATGTCAAAGATCAGCGTCTTTACAAGATCGGAGATATTTTACGCGTGCAACTCACGGAAGTGAAGGAAGCAACACGTAGTATTATgg                                                                                                                                                                                                                        <  1:387294/134‑1 (MQ=255)                                                                                                                            acaacaAAGAGGAAATTGTTATCAATACCGACGAAATCGCCTTCTATGTCAAAGATCAGCGTCTTTACAAGATCGGAGATATTTTACGCGTGCAACTCACGGAAGTGAAGGAAGCAACACGTAGTATTATGGGGATGGTCATCAAGTAAAAAAGTGCGGTGAATCTTaa                                                                                                                                                                                   >  1:237804/1‑169 (MQ=255)                                                                                                                               acaaAGAGGAAATTGTTATCAATACCGACGAAATCGCCTTCTATGTCAAAGATCAGCGTCTTTACAAGATCGGAGATATTTTACGCGTGCAACTCACGGAAGTGAAGGAAGCAACACGTAGTATTATGGGGATGGTCATCAAGTAAAAAAGTGCGGTGAATCTTAAAGATGTTTTCAAAACGCTCGAAAAAATCACCGCACTTTTGACC‑AAAAAAATCCTGCTTAACGATTTATTTAAGCAGGATTAtttt                                                                                             <  1:691665/251‑1 (MQ=255)                                                                                                                                    gagGAAATTGTTATCAATACCGACGAAATCGCCTTCTATGTCAAAGATCAGCGTCTTTACAAGATCGGAGATATTTTACGCGTGCAACTCACGGAAGTGAAGGAAGCAACACGTAGTATTATGGGGATGGTCATCAAGTAAAAAAGTGCGGTGAATCTTAAAGATGTTTTCAAAACGCTCGAAAAAATCACCGCACTTTTGACC‑AAAAAAATCCTGCTTAACGATTTATTTAAGCAGGATTATTTTTTTAg                                                                                        >  1:701366/1‑251 (MQ=255)                                                                                                                                        aaaTTGTTATCAATACCGACGAAATCGCCTTCTATGTCAAAGATCAGCGTCTTTACAAGATCGGAGATATTTTACGCGTGCAACTCACGGAAGTGAAGGAAGCAACACGTAGTATTATGGGGATGGTCATCAAGTAAAAAAGTGCGGTGAATCTTAAAGATGTTTTCAAAACGCTCGAAAAAATCACCGCACTTTTGAcc                                                                                                                                        >  1:154977/1‑200 (MQ=255)                                                                                                                                          aTTGTTATCAATACCGACGAAATCGCCTTCTATGTCAAAGATCAGCGTCTTTACAAGATCGGAGATATTTTACGCGTGCAACTCACGGAAGTGAAGGGAGCAACACGTAGTATTATGGGGATGGTCATCAAGTAAAAAAGTGCGGTGAATCTTAAAGAt                                                                                                                                                                               >  1:627451/1‑159 (MQ=255)                                                                                                                                          aTTGTTATCAATACCGACGAAATCGCCTTCTATGTCAAAGATCAGCGTCTTTACAAGATCGGAGATATTTTACGCGTGCAACTCACGGAAGTGAAGGAAGCAACACGTAGTATTATGGGGATGGTCATCAAGTAAAAAAGTGCGGTGAATCTTAAAGATGTTTTCAAAACGCTCGAAAAAATCACCGCACTTTTGACC‑AAAAAAATCCTGCTTAACGATTTATTTAAGCAGGATTATTTTTTTAGCtaatt                                                                                  >  1:745867/1‑251 (MQ=255)                                                                                                                                           ttGTTATCAATACCGACGAAATCGCCTTCTATGTCAAAGATCAGCGTCTTTACAAGATCGGAGATATTTTACGCGTGCAACTCACGGAAGTGAAGGAAGCAACACGTAGTATTATGGGGATGGTCATCAAGTAAAAAAGTGCGGTGAATCTTAAAGATGTTTTCAAAACGCTCGAAAAAATCACCGCACTTTTGACCAAAAAAAATCCTGCTTAACGATTTATTTAAGc                                                                                                        >  1:342860/1‑229 (MQ=255)                                                                                                                                             gTTATCAATACCGACGAAATCGCCTTCTATGTCAAAGATCAGCGTCTTTACAAGATCGGAGATATTTTACGCGTGCAACTCACGGAAGTGAAGGAAGCAACACGTAGTATTATGGGGATGGTCATCAAGTAAAAAAGTGCGGTGAATCTTAAAGATGTTTTCAAAACGCTCGAAAAAATCACCGCACTTTTGACC‑AAAAAAATCCTGCTTAACGATTTATTTAAGCAGGat                                                                                                   >  1:234111/1‑231 (MQ=255)                                                                                                                                             gTTATCAATACCGACGAAATCGCCTTCTATGTCAAAGATCAGCGTCTTTACAAGATCGGAGATATTTTACGCGTGCAACTCACGGAAGTGAAGGAAGCAACACGTAGTATTATGGGGATGGTCATCAAGTAAAAAAGTGCGGTGAATCTTAAAGATGTTTTCAAAACGCTCGAAAAAATCACCGCACTTTTGACC‑AAAAAAATCCTGCTTAACGATTTATTTAAGCAGGATTATTTTTTTAGCtaattaat                                                                               >  1:579277/1‑251 (MQ=255)                                                                                                                                               tATCAATACCGACGAAATCGCCTTCTATGTCAAAGATCAGCGTCTTTACAAGATCGGAGATATTTTACGCGTGCAACTCACGGAAGTGAAGGAAGCAACACGTAGTATTATGGGGATGGTCATCAAGTAAAAAAGTGCGGTGAATCTTAAAGATGTTTTCAAAACGCTCGAAAAAATCACCGCACTTTTGACC‑AAAAAAATCCTGCTTAACGATTTATTTAAGCAGGATTATTTTTTTAGCTAattaatta                                                                             >  1:293630/1‑251 (MQ=255)                                                                                                                                               tATCAATACCGACGAAATCGCCTTCTATGTCAAAGATCAGCGTCTTTACAAGATCGGAGATATTTTACGCGTGCAACTCACGGAAGTGAAGGAAGCAACACGTAGTATTATGGGGATGGTCATCAAGTAAAAAAGTGCGGTGAATCTTAAAGATGTTTTCAAAACGCTCGAAAAAATCACCGCACTTTTGACC‑AAAAAAATCCTGCTTAACGATTTATTTAAGCAGGATTATTTTTTTAGCTAattaatta                                                                             >  1:542850/1‑251 (MQ=255)                                                                                                                                               tATCAATACCGACGAAATCGCCTTCTATGTCAAAGATCAGCGTCTTTACAAGATCGGAGATATTTTACGCGTGCAACTCACGGAAGTGAAGGAAGCAACACGTAGTATTATGGGGATGGTCATCAAGTAAAAAAGTGCGGTGAATCTTAAAGATGTTTTCAAAACGCTCAAAAAAATCACCGCACTTTTGAcc                                                                                                                                        >  1:440837/1‑193 (MQ=255)                                                                                                                                                             aaaTCGCCTTCTATGTCAAAGATCAGCGTCTTTACAAGATCGGAGATATTTTACGCGTGCAACTCACGGAAGTGAAGGAAGCAACACGTAGTATTATGGGGATGGTCATCAAGt                                                                                                                                                                                                         <  1:620660/114‑1 (MQ=255)                                                                                                                                                                 cGCCTTCTATGTCAAAGATCAGCGTCTTTACAAGATCGGAGATATTTTACGCGTGCAACTCACGGAAGTGAAGGAAGCAACACGTAGTATTATGGGGATGGTCATCAAGTAAAAAAGTGCGGTGAATCTTAAAGATGTTTTCAAAACGCTCGAAAAAATCACCGCACTTTTGACC‑AAAAAAATCCTGCTTAACGATTTATTTAAGCAGGATTATTTTTTTAGCTAATTAATTAGCTTGCACAAGCTTGACa                                                           <  1:96483/251‑1 (MQ=255)                                                                                                                                                                   ccTTCTATGTCAAAGATCAGCGTCTTTACAAGATCGGAGATATTTTACGCGTGCAACTCACGGAAGTGAAGGAAGCAACACGTAGTATTATGGGGATGGTCATCAAGTAAAAAAGTGCGGTGAATCTTAAAGATGTTTTCAAAACGCTCGAAAAAATCACCGCACTTTTGACC‑AAAAAAATCCTGCTTAACGATTTATTTAAGCAGGATTATTTTTTTAGCTAATTAATTAGCTTGCACAAGCTTGACAAg                                                         >  1:837453/1‑251 (MQ=255)                                                                                                                                                                       cTATGTCAAAGATCAGCGTCTTTACAAGATCGGAGATATTTTACGCGTGCAACTCACGGAAGTGAAGGAAGCAACACGTAGTATTATGGGGATGGTCATCAAGTAAAAAAGTGCGGTGAATCTTAAAGATGTTTTCAAAACGCTCGAAAAAATCACCGCACTTTTGACC‑AAAAAAATCCTGCTTAACGATTTATTTAAGCAGGATTATTTTTTTAGCTAATTAATTAGCTTGCACAAGCTTGACAAGCTg                                                      >  1:574535/1‑250 (MQ=255)                                                                                                                                                                           gTCAAAGATCAGCGTCTTTACAAGATCGGAGATATTTTACGCGTGCAACTCACGGAAGTGAAGGAAGCAACACGTAGTATTATGGGGATGGTCATCAAg                                                                                                                                                                                                          <  1:397799/99‑1 (MQ=255)                                                                                                                                                                           gTCAAAGATCAGCGTCTTTACAAGATCGGAGATATTTTACGCGTGCAACTCACGGAAGTGAAGGAAGCAACACGTAGTATTATGGGGATGGTCATCAAGTAAAAAAGTGCGGTGAATCTTAAAGATGTTTTCAAAACGCTCGAAAAAATCACCGCACTTTTGACC‑AAAAAAATCCTGCTTAACGATTTATTTAAGCAGGATTATTTTTTTAGCTAATTAATTAGCTTGCACAAGCTTGACAAGCTGCCTgg                                                 >  1:535127/1‑251 (MQ=255)                                                                                                                                                                            tCAAAGATCAGCGTCTTTACAAGATCGGAGATATTTTACGCGTGCAACTCACGGAAGTGAAGGAAGCAACACGTAGTATTATGGGGATGGTCATCAAGTAAAAAAGTGCGGTGAATCTTAAAGATGTTTTCAAAACGCTCGAAAAAATCACCGCACTTTTGACC‑AAAAAAATCCTGCTTAACGATTTATTTAAGCAGGATTATTTTTTTAGCTAATTAATTAGCTTGCACAAGCTTGACAAGCTGCCTGGa                                                <  1:661005/251‑1 (MQ=255)                                                                                                                                                                              aaaGATCAGCGTCTTTACAAGATCGGAGATATTTTACGCGTGCAACTCACGGAAGTGAAGGAAGCAACACGTAGTATTATGGGGATGGTCATCAAGTAAAAAAGTGCGGTGAATCTTAAAGATGTTTTCAAAACGCTCGAAAAAATCACCGCACTTTTGACC‑AAAAAAATCCTGCTTAACGATTTATTTAAGCAGGATTATTTTTTTAGCTAATTAATTAGCTTGCACAAGCTTGACAAGCTGCCTGGAAc                                              <  1:401626/251‑1 (MQ=255)                                                                                                                                                                                          cTTTACAAGATCGGAGATATTTTACGCGTGCAACTCACGGAAGTGAAGGAAGCAACACGTAGTATTATGGGGATGGTCATCAAGTAAAAAAGTGCGGTGAATCTTAAAGATGTTTTCAAAACGCTCGAAAAAATCACCGCACTTTTGACC‑AAAAAAATCCTGCTTAACGatttattt                                                                                                            <  1:523742/177‑1 (MQ=255)                                                                                                                                                                                          cTTTACAAGATCGGAGATATTTTACGCGTGCAACTCACGGAAGTGAAGGAAGCAACACGTAGTATTATGGGGATGGTCATCAAGTAAAAAAGTGCGGTGAATCTTAAAGATGTTTTCAAAACGCTCGAAAAAATCACCGCACTTTTGACC‑AAAAAAATCCTGCTTAACGATTTATTTAAGCAGGATTAttt                                                                                              >  1:400109/1‑191 (MQ=255)                                                                                                                                                                                            ttACAAGATCGGAGATATTTTACGCGTGCAACTCACGGAAGTGAAGGAAGCAACACGTAGTATTATGGGGATGGTCATCAAGTAAAAAAGTGCGGTGAATCTTAAAGATGTTTTCAAAACGCTCGAAAAAATCACCGCACTTTTGACC‑AAAAAAATCCTGCTTAACGATTTATTTAAGCAGGattatt                                                                                               >  1:474951/1‑188 (MQ=255)                                                                                                                                                                                            ttACAAGATCGGAGATATTTTACGCGTGCAACTCACGGAAGTGAAGGAAGCAACACGTAGTATTATGGGGATGGTCATCAAGTAAAAAAGTGCGGTGAATCTTAAAGATGTTTTCAAAACGCTCGAAAAAATCACCGCACTTTTGACC‑AAAAAAATCCTGCTTAACGATTTATTTAAGCAGGATTATTTTTTTAGCTAATTAATTAGCTTGCACAAGCTTGACAAGCTGCCTGGAAc                                              <  1:15656/237‑1 (MQ=255)                                                                                                                                                                                                   aTCGGAGATATTTTACGCGTGCAACTCACGGAAGTGAAGGAAGCAACACGTAGTATTATGGGGATGGTCATCAAGTAAAAAAGTGCGGTGAATCTTAAAGATGTTTTCAAAACGCTCGAAAAAATc                                                                                                                                                       >  1:834277/1‑126 (MQ=255)                                                                                                                                                                                                   aTCGGAGATATTTTACGCGTGCAACTCACGGAAGTGAAGGAAGCAACACGTAGTATTATGGGGATGGTCATCAAGTAAAAAAGTGCGGTGAATCTTAAAGATGTTTTCAAAACGCTCGAAAAAATCACCGCACTTTTGACC‑AAAAAAATCCTGCTTAACGATTTATTTAAGCAGGATTATTTTTTTAGCTAATTAATTAg                                                                            <  1:829652/200‑1 (MQ=255)                                                                                                                                                                                                       gagaTATTTTACGCGTGCAACTCACGGAAGTGAAGGAAGCAACACGTAGTATTATGGGGATGGTCATCAAGTAAAAAAGTGCGGTGAATCTTAAAGATGTTTTCAAAACGCTCGAAAAAATCACCGCACTTTTGACC‑AAAAAAATCCTGCTTAACGATTTATTTAAGCAGGATTATTTTTTTAGCTAATTAATTAGCTTGCACAAGCTTGACAAGCTGCCTGGAAc                                              >  1:379213/1‑226 (MQ=255)                                                                                                                                                                                                       gagaTATTTTACGCGTGCAACTCACGGAAGTGAAGGAAGCAACACGTAGTATTATGGGGATGGTCATCAAGTAAAAAAGTGCGGTGAATCTTAAAGATGTTTTCAAAACGCTCGAAAAAATCACCGCACTTTTGACC‑AAAAAAATCCTGCTTAACGATTTATTTAAGCAGGATTATTTTTTTAGCTAATTAATTAGCTTGCACAAGCTTGACAAGCTGCCTGGAACATCCAAACCAGTTTTTc                             <  1:554681/243‑1 (MQ=255)                                                                                                                                                                                                           tatTTTACGCGTGCAACTCACGGAAGTGAAGGAAGCAACACGTAGTATTATGGGGATGGTCATCAAGTAAAAAAGTGCGGTGAATCTTAAAg                                                                                                                                                                                 >  1:531561/1‑92 (MQ=255)                                                                                                                                                                                                           tatTTTACGCGTGCAACTCACGGAAGTGAAGGAAGCAACACGTAGTATTATGGGGATGGTCATCAAGTAAAAAAGTGCGGTGAATCTTAAAGATGTTTTCAAAACGCTCGAAAAAATCACCGCACTTTTGACC‑AAAAAAATCCTGCTTAACGATTTATTTAAGCAGGATTATTTTTTTAGCTAATTAATTAGCTTGCACAAGCTTGACAAGCTGCCTGGAACATCCAAACCAGTTTTTCTTGCTCTTTTAt                 >  1:325917/1‑251 (MQ=255)                                                                                                                                                                                                            atTTTACGCGTGCAACTCACGGAAGTGAAGGAAGCAACACGTAGTATTATGGGGATGGTCATCAAGTAAAAAAGTGCGGTGAATCTTAAAGATGTTTTCAAAACGCTCGAAAAAATCACCGCACTTTTGACC‑AAAAAAATCCTGCTTAACGATTTATTTAAGCAGGATTATTTTTTTAGCTAATTAATTAGCTTGCACAAGCTTGACAAGCTGCCTGGAACATCCAAACCAGTTTTTCTTGCTCTTTTATg                <  1:842594/251‑1 (MQ=255)                                                                                                                                                                                                               ttACGCGTGCAACTCACGGAAGTGAAGGAAGCAACACGTAGTATTATGGGGATGGTCATCAAGTAAAAAAGTGCGGTGAATCTTAAAGATGTTTTCAAAACGCTCGAAAAAATCACCGCACTTTTGACC‑AAAAAAATCCTGCTTAACGATTTATTTAAGCAGGATTATTTTTTTAGCTAATTAATTAGCTTGCACAAGCTTGACAAGCTGCCTGGAACATCCAAACCAGTTTTTCTTGCTCTTTTATGTaa             <  1:53474/251‑1 (MQ=255)                                                                                                                                                                                                               ttACGCGTGCAACTCACGGAAGTGAAGGAAGCAACACGTAGTATTATGGGGATGGTCATCAAGTAAAAAAGTGCGGTGAATCTTAAAGATGTTTTCAAAACGCTCGAAAAAATCACCGCACTTTTGACC‑AAAAAAATCCTGCTTAACGATTTATTTAAGCAGGATTATTTTTTTAGCTAATTAATTAGCTTGCACAAGCTTGACAAGCTGCCTGGAACATCCAAACCAGTTTTTCTTGCTCTTTTATGTaa             <  1:669532/251‑1 (MQ=255)                                                                                                                                                                                                                tACGCGTGCAACTCACGGAAGTGAAGGAAGCAACACGTAGTATTATGGGGATGGTCATCAAGTAAAAAAGTGCGGTGAATCTTAAAGATGTTTTCAAAACGCTCGAAAAAATCACCGCACTTTTGACC‑AAAAAAATCCTGCTTAACGATTTATTTAAGCAGGATTATTTTTTTAGCTAATTAATTAGCTTGCACAAGCTTGACAAGCTGCCTGGAACATCCAAACCAGTTTTTCTTGCTCTTTTATGTAAt            <  1:560768/251‑1 (MQ=255)                                                                                                                                                                                                                tACGCGTGCAACTCACGGAAGTGAAGGAAGCAACACGTAGTATTATGGGGATGGTCATCAAGTAAAAAAGTGCGGTGAATCTTAAAGATGTTTTCAAAACGCTCGAAAAAATCACCGCACTTTTGACC‑AAAAAAATCCTGCTTAACGATTTATTTAAGCAGGATTATTTTTTTAGCTAATTAATTAGCTTGCACAAGCTTGACAAGCTGCCTGGAACATCCAAACCAGTTTTTCTTGCTCTTTTATGTAAt            >  1:213181/1‑251 (MQ=255)                                                                                                                                                                                                                   gcgTGCAACTCACGGAAGTGAAGGAAGCAACACGTAGTATTATGGGGATGGTCATCAAGTAAAAAAGTGCGGTGAATCTTAAAGATGTTTTCAAAACGCTCGAAAAAATCACCGCACTTTTGACC‑AAAAAAATCCTGCTTAACGATTTATTTAAGCAGGATTATTTTTTTAGCTAATTAATTAGCTTGCACAAGCTTGACAAGCTGCCTGGAACATCCAAACCAGTTTTTCTTGCTCTTTTATGTAATCa          >  1:462463/1‑250 (MQ=255)                                                                                                                                                                                                                        cAACTCACGGAAGTGAAGGAAGCAACACGTAGTATTATGGGGATGGTCATCAAGTAAAAAAGTGCGGTGAATCTTAAAGATGTTTTCAAAACGCTCGAAAAAATCACCGCACTTTTGACC‑AAAAAAATCCTGCTTAACGATTTATTTAAGCAGGATTATTTTTTTAGCTAATTAATTAGCTTGCACAAGCTTGACAAGCTGCCTGGAACATCCAAACCAGTTTTTCTTGCTCTTTTATGTAATCACTCAtt    <  1:864623/251‑1 (MQ=255)                                                                                                                                                                                                                          aCTCACGGAAGTGAAGGAAGCAACACGTAGTATTATGGGGATGGTCATCAAGTAAAAAAGTGCGGTGAATCTTAAAGATGTTTTCAAAACGCTCGAAAAAATCACCGCACTTTTGACC‑AAAAAAATCCTGCTTAACGATTTATTTAAGCAGGATTATTTTTTTAGCTAATTAATTAGCTTGCACAAGCTTGACAAGCTGCCTGGAACATCCAAACCAGTTTTTCTTGCTCTTTTATGTAATCACTCATTtg  >  1:740916/1‑251 (MQ=255)                                                                                                                                                                                                                                     tGAAGGAAGCAACACGTAGTATTATGGGGATGGTCATCAAGTAAAAAAGTGCGGTGAATCTTAAAGATGTTTTCAAAACGCTCGAAAAAATCACCGCACTTTTGACC‑AAAAAAATCCTGCTTAACGATTTATTTAAGCAGGATTATTTTTTTAGCTAATTAATTAGCTTGCACAAGCTTGACAAGCTGCCTGGAACATCCAAACCAGTTTTTCTTGCTCTTTTATGt               >  1:851855/1‑227 (MQ=255)                                                                                                                                                                                                                                             gCAACACGTAGTATTATGGGGATGGTCATCAAGTAAAAAAGTGCGGTGAATCTTAAAGATGTTTTCAAAACGCTCGAAAAAATCACCGCACTTTTGACC‑AAAAAAATCCTGCTTAACGATTTATTTAAGCAGGATTATTTTTTTAGCTAATTAATTAGCTTGCACAAGCTTGACAAGCTGCCTGGAAc                                              >  1:804289/1‑188 (MQ=255)                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                  CCGATAAAGTTGCCGAAAAAGTGGAATTTGAAGCAGAAGTACAAGATATTACCCGTGGAGGTTTACGTGTTTTATTGTTGGCAAACGGCGCGTCCATATTTATTCCTGCCTCAACCTTGCACGACAACAAAGAGGAAATTGTTATCAATACCGACGAAATCGCCTTCTATGTCAAAGATCAGCGTCTTTACAAGATCGGAGATATTTTACGCGTGCAACTCACGGAAGTGAAGGAAGCAACACATAGTATTATGGGGATGGTCATCAAGTAAAAAAGTGCGGTGAATCTTAAAGATGTTTTCAAAACGCTCGAAAAAATCACCGCACTTTTGACC‑AAAAAAATCCTGCTTAACGATTTATTTAAGCAGGATTATTTTTTTAGCTAATTAATTAGCTTGCACAAGCTTGACAAGCTGCCTGGAACATCCAAACCAGTTTTTCTTGCTCTTTTATGTAATCACTCATTTG  >  1/1546980‑1547447

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑