Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,539,416	A→C	intergenic (‑94788/‑102348)	unknown ← / → unknown	tRNA‑Met‑CAT/tRNA‑Gly‑GCC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,539,416	0	A	C	100.0%	213.0 / NA	75	intergenic (‑94788/‑102348)	unknown/unknown	tRNA‑Met‑CAT/tRNA‑Gly‑GCC
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (38/37);  total (38/37)

TTCCAATTTACTTTTCAGTGCAACATCATTTGCCGTTACACTCATAGATATAGCCAAAAGAGAAAGCGTAGTAATAATTTTTTTCATTTCTGTTGTTCCTAAATAAGTGAAATTGAGCGTGAATTATAAAAGTAATTGACGCGTTATGCAAAATTCACAGAATTTCACAGTTGGAGTTTCGTTCGCTCTACCCAATTCCCGCAAAATCCCTTATAATCCCGCACTTAATTTACCTTTTTACGAAATTCATTATCATGTTTGAAATTAATCCTATCAAAAATAAAATCGCCGATTTAACCGACCGCACTTCCGTGCTTCGGGGGTATCTTTGACTTCGACGCCAAACTGGAGCGTTTAGAAGAAGTCAACGCCGAACTGGAACAGCCTGACGTATGGAACGATGCAGAGAAAGCGCAGGCGCTCGGCAAAGAACGCGTTTCTTTAGAACTGGTGGTCAACACCATTAAAAATCTGGAAC  >  1/1539173‑1539650                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                           ttCCAATTTACTTTTCAGTGCAACATCATTTGCCGTTACACTCATAGATATAGCCAAAAGAGAAAGCGTAGTAATAATTTTTTTCATTTCTGTTGTTCCTAAATAAGTGAAATTGAGCGTGAATTATAAAAGTAATTGACGCGTTATGCAAAATTCACAGAATTTCACAGTTGGAGTTTCGTTCGCTCTACCCAATTCCCGCAAAATCCCTTATAATCCCGCACTTAATTTACCTTTTTACGAcattcatt                                                                                                                                                                                                                                     <  1:539447/251‑1 (MQ=255) ttCCAATTTACTTTTCAGTGCAACATCATTTGCCGTTACACTCATAGATATAGCCAAAAGAGAAAGCGTAGTAATAATTTTTTTCATTTCTGTTGTTCCTAAATAAGTGAAATTGAGCGTGAATTATAAAAGTAATTGACGCGTTATGCAAAATTCACAGAATTTCACAGTTGGAGTTTCGTTCGCTCTACCCAATTCCCGCAAAATCCCTTATAATCCCGCACTTAATTTACCTTTTTACGAcattcatt                                                                                                                                                                                                                                     <  1:652688/251‑1 (MQ=255)                     cAACATCATTTGCCGTTACACTCATAGATATAGCCAAAAGAGAAAGCGTAGTAATAATTTTTTTCATTTCTGTTGTTCCTAAATAAGTGAAATTGAGCGTGAATTATAAAAGTAATTGACGCGTTATGCAAAATTCACAGAATTTCACAGTTGGAGTTTCGTTCGCTCTACCCAATTCCCGCAAAATCCCTTATAATCCCGCACTTAATTTACCTTTTTACGACATTCATTATCATGTTTGAAATTAATc                                                                                                                                                                                                                  <  1:392198/250‑1 (MQ=255)                     cAACATCATTTGCCGTTACACTCATAGATATAGCCAAAAGAGAAAGCGTAGTAATAATTTTTTTCATTTCTGTTGTTCCTAAATAAGTGAAATTGAGCGTGAATTATAAAAGTAATTGACGCGTTATGCAAAATTCACAGAATTTCACAGTTGGAGTTTCGTTCGCTCTACCCAATTCCCGCAAAATCCCTTATAATCCCGCACTTAATTTACCTTTTTACGACATTCATTATCATGTTTGAAATTAATc                                                                                                                                                                                                                  <  1:678199/250‑1 (MQ=255)                           catTTGCCGTTACACTCATAGATATAGCCAAAAGAGAAAGCGTAGTAATAATTTTTTTCATTTCTGTTGTTCCTAAATAAGTGAAATTGAGCGTGAATTATAAAAGTAATTGACGCGTTATGCAAAATTCACAGAATTTCACAGTTGGAGTTTCGTTCGCTCTACCCAATTCCCGCAAAATCCCTTATAATCCCGCACTTAATTTACCTTTTTACGACATTCATTATCATGTTTGAAATTAATCCTATCaa                                                                                                                                                                                                           <  1:584787/251‑1 (MQ=255)                           catTTGCCGTTACACTCATAGATATAGCCAAAAGAGAAAGCGTAGTAATAATTTTTTTCATTTCTGTTGTTCCTAAATAAGTGAAATTGAGCGTGAATTATAAAAGTAATTGACGCGTTATGCAAAATTCACAGAATTTCACAGTTGGAGTTTCGTTCGCTCTACCCAATTCCCGCAAAATCCCTTATAATCCCGCACTTAATTTACCTTTTTACGACATTCATTATCATGTTTGAAATTAATCCTATCaa                                                                                                                                                                                                           <  1:658573/251‑1 (MQ=255)                                    ttACACTCATAGATATAGCCAAAAGAGAAAGCGTAGTAATAATTTTTTTCATTTCTGTTGTTCCTAAATAAGTGAAATTGAGCGTGAATTATAAAAGTAATTGACGCGTTATGCAAAATTCACAGAATTTCACAGTTGGAGTTTCGTTCGCTCTACCCAATTCCCGCAAAATCCCTTATAATCCCGCACTTAATTTACCTTTTTACGACATTCATTATCATGTTTGAAATTAATCCTATCAaaaataaaat                                                                                                                                                                                                  <  1:595749/251‑1 (MQ=255)                                       cacTCATAGATATAGCCAAAAGAGAAAGCGTAGTAATAATTTTTTTCATTTCTGTTGTTCCTAAATAAGTGAAATTGAGCGTGAATTATAAAAGTAATTGACGCGTTATGCAAAATTCACAGAATTTCACAGTTGGAGTTTCGTTCGCTCTACCCAATTCCCGCAAAATCCCTTATAATCCCGCACTTAATTTACCTTTTTACGACATTCATTATCATGTTTGAAATTAATCCTATCAAAAATAAAATCg                                                                                                                                                                                                >  1:109493/1‑250 (MQ=255)                                         cTCATAGATATAGCCAAAAGAGAAAGCGTAGTAATAATTTTTTTCATTTCTGTTGTTCCTAAATAAGTGAAATTGAGCGTGAATTATAAAAGTAATTGACGCGTTATGCAAAATTCACAGAATTTCACAGTTGGAGTTTCGTTCGCTCTACCCAATTCCCGCAAAATCCCTTATAATCCCGCACTTAATTTACCTTTTTACGACATTCATTATCATGTTTGAAATTAATc                                                                                                                                                                                                                  >  1:605246/1‑230 (MQ=255)                                         cTCATAGATATAGCCAAAAGAGAAAGCGTAGTAATAATTTTTTTCATTTCTGTTGTTCCTAAATAAGTGAAATTGAGCGTGAATTATAAAAGTAATTGACGCGTTATGCAAAATTCACAGAATTTCACAGTTGGAGTTTCGTTCGCTCTACCCAATTCCCGCAAAATCCCTTATAATCCCGCACTTAATTTACCTTTTTACGACATTCATTATCATGTTTGAAATTAATCCTATCAAAAATAAAATCGCCg                                                                                                                                                                                             >  1:589542/1‑251 (MQ=255)                                         cTCATAGATATAGCCAAAAGAGAAAGCGTAGTAATAATTTTTTTCATTTCTGTTGTTCCTAAATAAGTGAAATTGAGCGTGAATTATAAAAGTAATTGACGCGTTATGCAAAATTCACAGAATTTCACAGTTGGAGTTTCGTTCGCTCTACCCAATTCCCGCAAAATCCCTTATAATCCCGCACTTAATTTACCTTTTTACGACATTCATTATCATGTTTGAAATTAATCCTATCAAAAATAAAATCGCCg                                                                                                                                                                                             >  1:381404/1‑251 (MQ=255)                                           cATAGATATAGCCAAAAGAGAAAGCGTAGTAATAATTTTTTTCATTTCTGTTGTTCCTAAATAAGTGAAATTGAGCGTGAATTATAAAAGTAATTGACGCGTTATGCAAAATTCACAGAATTTCACAGTTGGAGTTTCGTTCGCTCTACCCAATTCCCGCAAAATCCCTTATAATCCCGCACTTAATTTACCTTTTTACGACATTCATTATCATGTTTGAAATTAATCCTATCAAAAATAAAATCGCCGAt                                                                                                                                                                                           >  1:23101/1‑251 (MQ=255)                                               gATATAGCCAAAAGAGAAAGCGTAGTAATAATTTTTTTCATTTCTGTTGTTCCTAAATAAGTGAAATTGAGCGTGAATTATAAAAGTAATTGACGCGTTATGCAAAATTCACAGAATTTCACAGTTGGAGTTTCGTTCGCTCTACCCAATTCCCGCAAAATCCCTTATAATCCCGCACTTAATTTACCTTTTTACGACATTCATTATCATGTTTGAAATTAATCCTATCAAAAATAAAATCGCCGATTTaa                                                                                                                                                                                       >  1:708199/1‑251 (MQ=255)                                                           agagAAAGCGTAGTAATAATTTTTTTCATTTCTGTTGTTCCTAAATAAGTGAAATTGAGCGTGAATTATAAAAGTAATTGACGCGTTATGCAAAATTCACAGAATTTCACAGTTGGAGTTTCGTTCGCTCTACCCAATTCCCGCAAAATCCCTTATAATCCCGCACTTAATTTACCTTTTTACGACATTCATTATCATGTTTGAAATTAATCCTATCAAAAATAAAATCGCCGATTTAACCGACCGCACtt                                                                                                                                                                           >  1:813542/1‑251 (MQ=255)                                                           agagAAAGCGTAGTAATAATTTTTTTCATTTCTGTTGTTCCTAAATAAGTGAAATTGAGCGTGAATTATAAAAGTAATTGACGCGTTATGCAAAATTCACAGAATTTCACAGTTGGAGTTTCGTTCGCTCTACCCAATTCCCGCAAAATCCCTTATAATCCCGCACTTAATTTACCTTTTTACGACATTCATTATCATGTTTGAAATTAATCCTATCAAAAATAAAATCGCCGATTTAACCGACCGCACtt                                                                                                                                                                           >  1:151890/1‑251 (MQ=255)                                                             agaAAGCGTAGTAATAATTTTTTTCATTTCTGTTGTTCCTAAATAAGTGAAATTGAGCGTGAATTATAAAAGTAATTGACGCGTTATGCAAAATTCACAGAATTTCACAGTTGGAGTTTCGTTCGCTCTACCCAATTCCCGCAAAATCCCTTATAATCCCGCACTTAATTTACCTTTTTACGACATTCATTATCATGTTTGAAATTAATCCTATCAAAAATAAAATCGCCGATTTAACCGACCGCACTTc                                                                                                                                                                          <  1:712111/250‑1 (MQ=255)                                                             agaAAGCGTAGTAATAATTTTTTTCATTTCTGTTGTTCCTAAATAAGTGAAATTGAGCGTGAATTATAAAAGTAATTGACGCGTTATGCAAAATTCACAGAATTTCACAGTTGGAGTTTCGTTCGCTCTACCCAATTCCCGCAAAATCCCTTATAATCCCGCACTTAATTTACCTTTTTACGACATTCATTATCATGTTTGAAATTAATCCTATCAAAAATAAAATCGCCGATTTAACCGACCGCACTTc                                                                                                                                                                          <  1:419117/250‑1 (MQ=255)                                                                     tagtaATAATTTTTTTCATTTCTGTTGTTCCTAAATAAGTGAAATTGAGCGTGAATTATAAAAGTAATTGACGCGTTATGCAAAATTCACAGAATTTCACAGTTGGAGTTTCGTTCGCTCTACCCAATTCCCGCAAAATCCCTTATAATCCCGCACTTAATTTACCTTTTTACGACATTCATTATCATGTTTGAAATTAATCCTATCAAAAATAAAATCGCCGATTTAACCGACCGCACTTCCGTGCTTCg                                                                                                                                                                 >  1:797317/1‑251 (MQ=255)                                                                       gtaATAATTTTTTTCATTTCTGTTGTTCCTAAATAAGTGAAATTGAGCGTGAATTATAAAAGTAATTGACGCGTTATGCAAAATTCACAGAATTTCACAGTTGGAGTTTCGTTCGCTCTACCCAATTCCCGCAAAATCCCTTATAATCCCGCACTTAATTTACCTTTTTACGACATTCATTATCa                                                                                                                                                                                                                                 <  1:346387/185‑1 (MQ=255)                                                                       gtaATAATTTTTTTCATTTCTGTTGTTCCTAAATAAGTGAAATTGAGCGTGAATTATAAAAGTAATTGACGCGTTATGCAAAATTCACAGAATTTCACAGTTGGAGTTTCGTTCGCTCTACCCAATTCCCGCAAAATCCCTTATAATCCCGCACTTAATTTACCTTTTTACGACATTCATTATCATGTTTGaaa                                                                                                                                                                                                                        >  1:395558/1‑194 (MQ=255)                                                                              tttttttCATTTCTGTTGTTCCTAAATAAGTGAAATTGAGCGTGAATTATAAAAGTAATTGACGCGTTATGCAAAATTCACAGAATTTCACAGTTGGAGTTTCGTTCGCTCTACCCAATTCCCGCAAAATCCCTTATAATCCCGCACTTAATTTACCTTTTTACGACATTCATTATc                                                                                                                                                                                                                                  <  1:512043/177‑1 (MQ=255)                                                                                 ttttCATTTCTGTTGTTCCTAAATAAGTGAAATTGAGCGTGAATTATAAAAGTAATTGACGCGTTATGCAAAATTCACAGAATTTCACAGTTGGAGTTTCGTTCGCTCTACCCAATTCCCGCAAAATCCCTTATAATCCCGCACTTAATTTACCTTTTTACGACATTCATTATCATGTTTGAAATTAATCCTATCAAAAATAAAATCGCCGATTTAACCGACCGCACTTCCGTGCTTCGGGGGTATCTTTg                                                                                                                                                     <  1:463234/251‑1 (MQ=255)                                                                                          cTGTTGTTCCTAAATAAGTGAAATTGAGCGTGAATTATAAAAGTAATTGACGCGTTATGCAAAATTCACAGAATTTCACAGTTGGAGTTTCGTTCGCTCTACCCAATTCCCGCAAAATCCCTTATAATCCCGCACTTAATTTACCTTTTTACGACATTCATTATCATGTTTGAAATTAATCCTATCAAAAATAAAATCGCCGATTTAACCGACCGCACTTCCGTGCTTCGGGGGTATCTTTGACTTCGACg                                                                                                                                            >  1:813205/1‑251 (MQ=255)                                                                                                     aaaTAAGTGAAATTGAGCGTGAATTATAAAAGTAATTGACGCGTTATGCAAAATTCACAGAATTTCACAGTTGGAGTTTCGTTCGCTCTACCCAATTCCCGCAAAATCCCTTATAATCCCGCACTTAATTTACCTTTTTACGACATTCATTATCATGTTTGAAATTAATCCTATCAAAAATAAAATCGCCGATTTAACCGACCGCACTTCCGTGCTTCGGGGGTATCTTTGACTTCGACGCCAAACTGGAg                                                                                                                                 <  1:566866/251‑1 (MQ=255)                                                                                                          aGTGAAATTGAGCGTGAATTATAAAAGTAATTGACGCGTTATGCAAAATTCACAGAATTTCACAGTTGGAGTTTCGTTCGCTCTACCCAATTCCCGCAAAATCCCTTATAATCCCGCACTTAATTTACCTTTTTACGACATTCATTATCATGTTTGAAATTAATCCTATCAAAAATAAAATCGCCGATTTAACCGACCGCACTTCCGTGCTTCGGGGGTATCTTTGACTTCGACGCCAAACTGGAGCGttt                                                                                                                            >  1:85955/1‑251 (MQ=255)                                                                                                           gTGAAATTGAGCGTGAATTATAAAAGTAATTGACGCGTTATGCAAAATTCACAGAATTTCACAGTTGGAGTTTCGTTCGCTCTACCCAATTCCCGCAAAATCCCTTATAATCCCGCACTTAATTTACCTTTTTACGACATTCATTATCATGTTTGAAATTAATCCTATCAAAAATAAAATCGCCGATTTAACCGACCGCACTTCCGTGCTTCGGGGGTATCTTTGACTTCGACGCCAAACTGGAGCGTTTa                                                                                                                           >  1:899555/1‑251 (MQ=255)                                                                                                           gTGAAATTGAGCGTGAATTATAAAAGTAATTGACGCGTTATGCAAAATTCACAGAATTTCACAGTTGGAGTTTCGTTCGCTCTACCCAATTCCCGCAAAATCCCTTATAATCCCGCACTTAATTTACCTTTTTACGACATTCATTATCATGTTTGAAATTAATCCTATCAAAAATAAAATCGCCGATTTAACCGACCGCACTTCCGTGCTTCGGGGGTATCTTTGACTTCGACGCCAAACTGGAGCGTTTa                                                                                                                           >  1:244230/1‑251 (MQ=255)                                                                                                           gTGAAATTGAGCGTGAATTATAAAAGTAATTGACGCGTTATGCAAAATTCACAGAATTTCACAGTTGGAGTTTCGTTCGCTCTACCCAATTCCCGCAAAATCCCTTATAATCCCGCACTTAATTTACCTTTTTACGACATTCATTATCATGTTTGAAATTAATCCTATCAAAAATAAAATCGCCGATTTAACCGACCGCACTTCCGTGCTTCGGGGGTATCTTTGACTTCGACGCCAAACTGGAGCGTTTa                                                                                                                           >  1:692810/1‑251 (MQ=255)                                                                                                              aaaTTGAGCGTGAATTATAAAAGTAATTGACGCGTTATGCAAAATTCACAGAATTTCACAGTTGGAGTTTCGTTCGCTCTACCCAATTCCCGCAAAATCCCTTATAATCCCGCACTTAATTTACCTTTTTACGACATTCATTATCATGTTTGAAATTAATCCTATCAAAAATAAAATCGCCGATTTAACCGACCGCACTTCCGTGCTTCGGGGGTATCTTTGACTTCGACGCCAAACTGGAGCGTTTagaa                                                                                                                        >  1:718413/1‑251 (MQ=255)                                                                                                               aaTTGAGCGTGAATTATAAAAGTAATTGACGCGTTATGCAAAATTCACAGAATTTCACAGTTGGAGTTTCGTTCGCTCTACCCAATTCCCGCAAAATCCCTTATAATCCCGCACTTAATTTACCTTTTTACGACATTCATTATCATGTTTGAAATTAATCCTATCAAAAATAAAATCGCCGATTTAACCGACCGCACTTCCGTGCTTCGGGGGTATCTTTGACTTCGACGCCAAACTGGAGCGTTTagaag                                                                                                                       <  1:150287/251‑1 (MQ=255)                                                                                                               aaTTGAGCGTGAATTATAAAAGTAATTGACGCGTTATGCAAAATTCACAGAATTTCACAGTTGGAGTTTCGTTCGCTCTACCCAATTCCCGCAAAATCCCTTATAATCCCGCACTTAATTTACCTTTTTACGACATTCATTATCATGTTTGAAATTAATCCTATCAAAAATAAAATCGCCGATTTAACCGACCGCACTTCCGTGCTTCGGGGGTATCTTTGACTTCGACGCCAAACTGGAGCGTTTagaag                                                                                                                       <  1:154491/251‑1 (MQ=255)                                                                                                                     gCGTGAATTATAAAAGTAATTGACGCGTTATGCAAAATTCACAGAATTTCACAGTTGGAGTTTCGTTCGCTCTACCCAATTCCCGCAAAATCCCTTATAATCCCGCACTTAATTTACCTTTTTACGACATTCATTATCATGTTTGAAATTAATCCTATCAAAAATAAAATCGCCGATTTAACCGACCGCACTTCCGTGCTTCGGGGGTATCTTTGACTTCGACGCCAAACTGGAg                                                                                                                                 <  1:564302/235‑1 (MQ=255)                                                                                                                      cGTGAATTATAAAAGTAATTGACGCGTTATGCAAAATTCACAGAATTTCACAGTTGGAGTTTCGTTCGCTCTACCCAATTCCCGCAAAATCCCTTATAATCCCGCACTTAATTTACCTTTTTACGACATTCATTATCATGTTTGAAATTAATCCTATCAAAAATAAAATCGCCGATTTAACCGACCGCACTTCCGTGCTTCGGGGGTATCTTTGACTTCGACGCCAAACTGGAGCGTTTAGAAGAAGTc                                                                                                                  <  1:134491/249‑1 (MQ=255)                                                                                                                            ttATAAAAGTAATTGACGCGTTATGCAAAATTCACAGAATTTCACAGTTGGAGTTTCGTTCGCTCTACCCAATTCCCGCAAAATCCCTTATAATCCCGCACTTAATTTACCTTTTTACGACATTCATTATCATGTTTGAAATTAATCCTATCAAAAATAAAATCGCCGATTTAACCGACCGCACTTCCGTGCTTCGGGGGTATCTTTGACTTCGACGCCAAACTGGAGCGTTTAGAAGAAGTCAACGCCGa                                                                                                          >  1:50985/1‑251 (MQ=255)                                                                                                                                 aaaGTAATTGACGCGTTATGCAAAATTCACAGAATTTCACAGTTGGAGTTTCGTTCGCTCTACCCAATTCCCGCAAAATCCCTTATAATCCCGCACTTAATTTACCTTTTTACGACATTCATTATCATGTTTGAAATTAATCCTATCAAAAATAAAATCGCCGATTTAACCGACCGCACTTCCGTGCTTCGGGGGTATCTTTGACTTCGACGCCAAACTGGAGCGTTTAGAAGAAGTCAACGCCGAACTgg                                                                                                     >  1:314293/1‑251 (MQ=255)                                                                                                                                  aaGTAATTGACGCGTTATGCAAAATTCACAGAATTTCACAGTTGGAGTTTCGTTCGCTCTACCCAATTCCCGCAAAATCCCTTATAATCCCGCACTTAATTTACCTTTTTACGACATTCATTATCATGTTTGAAATTAATCCTATCAAAAATAAAATCGCCGATTTAACCGACCGCACTTCCGTGCTTCGGGGGTATCTTTGACTTCGACGCCAAACTGGAGCGTTTAGAAGAAGTCAACGCCGAACTGGa                                                                                                    >  1:19636/1‑251 (MQ=255)                                                                                                                                  aaGTAATTGACGCGTTATGCAAAATTCACAGAATTTCACAGTTGGAGTTTCGTTCGCTCTACCCAATTCCCGCAAAATCCCTTATAATCCCGCACTTAATTTACCTTTTTACGACATTCATTATCATGTTTGAAATTAATCCTATCAAAAATAAAATCGCCGATTTAACCGACCGCACTTCCGTGCTTCGGGGGTATCTTTGACTTCGACGCCAAACTGGAGCGTTTAGAAGAAGTCAACGCCGAACTGGa                                                                                                    >  1:572113/1‑251 (MQ=255)                                                                                                                                              cgTTATGCAAAATTCACAGAATTTCACAGTTGGAGTTTCGTTCGCTCTACCCAATTCCCGCAAAATCCCTTATAATCCCGCACTTAATTTACCTTTTTACGACATTCATTATCATGTTTGAAATTAATCCTATCAAAAATAAAATCGCCGATTTAACCGACCGCACTTCCGTGCTTCGGGGGTATCTTTGACTTCGACGCCAAACTGGAGCGTTTAGAAGAAGTCAACGCCGAACTGGAACAGCCTGACGt                                                                                        <  1:644562/251‑1 (MQ=255)                                                                                                                                                  aTGCAAAATTCACAGAATTTCACAGTTGGAGTTTCGTTCGCTCTACCCAATTCCCGCAAAATCCCTTATAATCCCGCACTTAATTTACCTTTTTACGACATTCATTATCATGTTTGAAATTAATCCTATCAAAAATAAAATCGCCGATTTAACCGACCGCACTTCCGTGCTTCGGGGGTATCTTTGACTTCGACGCCAAACTGGAGCGTTTAGAAGAAGTCAACGCCGAACTGGAACAGCCTGACGTATgg                                                                                    <  1:109166/251‑1 (MQ=255)                                                                                                                                                           tCACAGAATTTCACAGTTGGAGTTTCGTTCGCTCTACCCAATTCCCGCAAAATCCCTTATAATCCCGCACTTAATTTACCTTTTTACGACATTCATTATCa                                                                                                                                                                                                                                 >  1:605713/1‑101 (MQ=255)                                                                                                                                                            cacaGAATTTCACAGTTGGAGTTTCGTTCGCTCTACCCAATTCCCGCAAAATCCCTTATAATCCCGCACTTAATTTACCTTTTTACGACATTCATTATCATGTTTGAAATTAATCCTATCAAAAATAAAATCGCCGATTTAACCGACCGCACTTCCGTGCTTCGGGGGTATCTTTGACTTCGACGCCAAACTGGAGCGTTTAGAAGAAGTCAACGCCGAACTGGAACAGCCTGACGTATGGAACGATGCag                                                                          <  1:347396/251‑1 (MQ=255)                                                                                                                                                                  aTTTCACAGTTGGAGTTTCGTTCGCTCTACCCAATTCCCGCAAAATCCCTTATAATCCCGCACTTAATTTACCTTTTTACGACATTCATTATCATGTTTGaaa                                                                                                                                                                                                                        <  1:537799/103‑1 (MQ=255)                                                                                                                                                                  aTTTCACAGTTGGAGTTTCGTTCGCTCTACCCAATTCCCGCAAAATCCCTTATAATCCCGCACTTAATTTACCTTTTTACGACATTCATTATCATGTTTGAAATTAATCCTATCAAAAATAAAATCGCCGATTTAACCGACCGCACTTCCGTGCTTCGGGGGTATCTTTGACTTCGACGCCAAACTGGAGCGTTTAGAAGAAGTCAACGCCGAACTGGAACAGCCTGACGTATGGAACGATGCAGAGAAAg                                                                    >  1:77070/1‑251 (MQ=255)                                                                                                                                                                     tCACAGTTGGAGTTTCGTTCGCTCTACCCAATTCCCGCAAAATCCCTTATAATCCCGCACTTAATTTACCTTTTTACGACATTCATTATCATGTTTGAAATTAATCCTATCAAAAATAAAATCGCCGATTTAACCGACCGCACTTCCGTGCTTCGGGGGTATCTTTGACTTCGACGCCAAACTGGAGCGTTTAGAAGAAGTCAACGCCGAACTGGAACAGCCTGACGTATGGAACGATGCAGAGAAAgcgc                                                                 <  1:457178/251‑1 (MQ=255)                                                                                                                                                                        caGTTGGAGTTTCGTTCGCTCTACCCAATTCCCGCAAAATCCCTTATAATCCCGCACTTAATTTACCTTTTTACGACATTCATTATCATGTTTGAAATTAATCCTATCAAAAATAAAATCGCCGATTTAACCGACCGCACTTCCGTGCTTCGGGGGTATCTTTGACTTCGACGCCAAACTGGAGCGTTTagaag                                                                                                                       >  1:284216/1‑194 (MQ=255)                                                                                                                                                                          gTTGGAGTTTCGTTCGCTCTACCCAATTCCCGCAAAATCCCTTATAATCCCGCACTTAATTTACCTTTTTACGACATTCATTATCATGTTTGAAAt                                                                                                                                                                                                                       >  1:559368/1‑96 (MQ=255)                                                                                                                                                                          gTTGGAGTTTCGTTCGCTCTACCCAATTCCCGCAAAATCCCTTATAATCCCGCACTTAATTTACCTTTTTACGACATTCATTATCATGTTTGAAATTAATCCTATCAAAAATAAAATCGCCGATTTAACCGACCGCACTTCCGTGCTTCGGGGGTATCTTTGACTTCGACGCCAAACTGGAGCGTTTAGAAGAAGTCAACGCCGAACTGGAACAGcc                                                                                              >  1:774686/1‑217 (MQ=255)                                                                                                                                                                                   tcgttcgCTCTACCCAATTCCCGCAAAATCCCTTATAATCCCGCACTTAATTTACCTTTTTACGACATTCATTATCATGTTTGAAATTAATCCTATCAAAAATAAAATCGCCGATTTAACCGACCGCACTTCCGTGCTTCGGGGGTATCTTTGACTTCGACGCCAAACTGGAGCGTTTAGAAGAAGTCAACGCCGAACTGGAACAGCCTGACGTATGGAACGATGCAGAGAAAGCGCAGGCGCTCGGCaaa                                                   >  1:412753/1‑251 (MQ=255)                                                                                                                                                                                         gCTCTACCCAATTCCCGCAAAATCCCTTATAATCCCGCACTTAATTTACCTTTTTACGACATTCATTATCATGTTTGAAATTAATCCTATCAAAAATAAAATCGCCGATTTAACCGACCGCACTTCCGTGCTTCGGGGGTATCTTTGACTTCGACGCCAAACTGGAGCGTTTAGAAGAAGTCAACGCCGAACTGGAACAGCCTGACGTATGGAACGATGCAGAGAAAGCGCAGGCGCTCGGc                                                      >  1:88963/1‑242 (MQ=255)                                                                                                                                                                                          ctctACCCAATTCCCGCAAAATCCCTTATAATCCCGCACTTAATTTACCTTTTTACGACATTCATTATCATGTTTGAAATTAATCCTATCAAAAATAAAATCGCCGATTTAACCGACCGCACTTc                                                                                                                                                                          <  1:867295/125‑1 (MQ=255)                                                                                                                                                                                          ctctACCCAATTCCCGCAAAATCCCTTATAATCCCGCACTTAATTTACCTTTTTACGACATTCATTATCATGTTTGAAATTAATCCTATCAAAAATAAAATCGCCGATTTAACCGACCGCACTTCCGTGCTTCGGGGGTATCTTTGACTTCGACGCCAAACTGGAGCGTTTAGAAGAAGTCAACGCCGAACTGGAACAGCCTGACGTATGGAACGATGCAGAGAAAGCGCAGGCGCTCGGCAAAGAAcgcg                                            >  1:418094/1‑251 (MQ=255)                                                                                                                                                                                            ctACCCAATTCCCGCAAAATCCCTTATAATCCCGCACTTAATTTACCTTTTTACGACATTCATTATCATGTTTGAAATTAATCCTATCAAAAATAAAATCGCCGATTTAACCGACCGCACTTCCGTGCTTCGGGGGTATCTTTGACTTCGACGCCAAACTGGAGCGTTTAGAAGAAGTCAACGCCGAACTGGAACAGCCTGACGTATGGAACGATGCAGAGAAAGCGCAGGCGCTCGGCAAAGAACGCGtt                                          >  1:269416/1‑251 (MQ=255)                                                                                                                                                                                              aCCCAATTCCCGCAAAATCCCTTATAATCCCGCACTTAATTTACCTTTTTACGACATTCATTATCATGTTTGAAATTAATCCTATCAAAAATAAAATCGCCGATTTAACCGACCGCACTTCCGTGCTTCGGGGGTATCTTTGACTTCGACGCCAAACTGGAGCGTTTAGAAGAAGTCAACGCCGAACTGGAACAGCCTGACg                                                                                         <  1:208230/202‑1 (MQ=255)                                                                                                                                                                                              aCCCAATTCCCGCAAAATCCCTTATAATCCCGCACTTAATTTACCTTTTTACGACATTCATTATCATGTTTGAAATTAATCCTATCAAAAATAAAATCGCCGATTTAACCGACCGCACTTCCGTGCTTCGGGGGTATCTTTGACTTCGACGCCAAACTGGAGCGTTTAGAAGAAGTCAACGCCGAACTGGAACAGCCTGACGTATgg                                                                                    >  1:109685/1‑207 (MQ=255)                                                                                                                                                                                              aCCCAATTCCCGCAAAATCCCTTATAATCCCGCACTTAATTTACCTTTTTACGACATTCATTATCATGTTTGAAATTAATCCTATCAAAAATAAAATCGCCGATTTAACCGACCGCACTTCCGTGCTTCGGGGGTATCTTTGACTTCGACGCCAAACTGGAGCGTTTAGAAGAAGTCAACGCCGAACTGGAACAGCCTGACGTATGGAACGATGCAGAGAAAGCGCAGGCGCTCGGCAAAGAACGCGttt                                         >  1:602089/1‑250 (MQ=255)                                                                                                                                                                                                      cccGCAAAATCCCTTATAATCCCGCACTTAATTTACCTTTTTACGACATTCATTATCATGTTTGAAATTAATCCTATCAAAAATAAAATCGCCGATTTAACCGACCGCACTTCCGTGCTTCGGGGGTATCTTTGACTTCGACGCCAAACTGGAGCGTTTAGAAGAAGTCAACGCCGAACTGGAACAGCCTGACGTATGGAACGATGCAGAGAAAGCGCAGGCGCTCGGCAAAGAACGCGTTTCTTTAGAAc                                <  1:293481/251‑1 (MQ=255)                                                                                                                                                                                                      cccGCAAAATCCCTTATAATCCCGCACTTAATTTACCTTTTTACGACATTCATTATCATGTTTGAAATTAATCCTATCAAAAATAAAATCGCCGATTTAACCGACCGCACTTCCGTGCTTCGGGGGTATCTTTGACTTCGACGCCAAACTGGAGCGTTTAGAAGAAGTCAACGCCGAACTGGAACAGCCTGACGTATGGAACGATGCAGAGAAAGCGCAGGCGCTCGGCAAAGAACGCGTTTCTTTAGAAc                                <  1:861438/251‑1 (MQ=255)                                                                                                                                                                                                      cccGCAAAATCCCTTATAATCCCGCACTTAATTTACCTTTTTACGACATTCATTATCATGTTTGAAATTAATCCTATCAAAAATAAAATCGCCGATTTAACCGACCGCACTTCCGTGCTTCGGGGGTATCTTTGACTTCGACGCCAAACTGGAGCGTTTAGAAGAAGTCAACGCCGAACTGGAACAGCCTGACGTATGGAACGATGCAGAGAAAGCGCAGGCGCTCGGCAAAGAACGCGTTTCTTTAGAAc                                <  1:605583/251‑1 (MQ=255)                                                                                                                                                                                                      cccGCAAAATCCCTTATAATCCCGCACTTAATTTACCTTTTTACGACATTCATTATCATGTTTGAAATTAATCCTATCAAAAATAAAATCGCCGATTTAACCGACCGCACTTCCGTGCTTCGGGGGTATCTTTGACTTCGACGCCAAACTGGAGCGTTTAGAAGAAGTCAACGCCGAACTGGAACAGCCTGACGTATGGAACGATGCAGAGAAAGCGCAGGCGCTCGGCAAAGAACGCGTTTCTTTAGAAc                                <  1:744019/251‑1 (MQ=255)                                                                                                                                                                                                         gCAAAATCCCTTATAATCCCGCACTTAATTTACCTTTTTACGACATTCATTATCATGTTTGAAATTAATCCTATCAAAAATAAAATCGCCGATTTAACCGACCGCACTTCCGTGCTTCGGGGGTATCTTTGACTTCGACGCCAAACTGGAGCGTTTAGAAGAAGTCAACGCCGAACTGGAACAGCCTGACGTATGGAACGATGCAGAGAAAGCGCAgg                                                              <  1:426177/218‑1 (MQ=255)                                                                                                                                                                                                              aTCCCTTATAATCCCGCACTTAATTTACCTTTTTACGACATTCATTATCATGTTTGAAATTAATCCTATCAAAAATAAAATCGCCGATTTAACCGACCGCACTTCCGTGCTTCGGGGGTATCTTTGACTTCGACGCCAAACTGGAGCGTTTAGAAGAAGTCAACGCCGAACTGGAACAGCCTGACGTATGGAACGATGCAGAGAAAGCGCAGGCGCTCGGCAAAGAACGCGTTTCTTTAGAACTGGTGGTc                        <  1:769515/251‑1 (MQ=255)                                                                                                                                                                                                                cccTTATAATCCCGCACTTAATTTACCTTTTTACGACATTCATTATCATGTTTGAAATTAATCCTATCAAAAATAAAATCGCCGATTTAACCGACCGCACTTCCGTGCTTCGGGGGTATCTTTGACTTCGACGCCAAACTGGAGCGTTTAGAAGAAGTCAACGCCGAACTGGAACAGCCTGACGTATGGAACGATGCAGAGAAAGCGCAGGCGCTCGGCAAAGAACGCGTTTCTTTAGAACTGGTGGTCaa                      >  1:282956/1‑251 (MQ=255)                                                                                                                                                                                                                  cTTATAATCCCGCACTTAATTTACCTTTTTACGACATTCATTATCATGTTTGAAATTAATCCTATCAAAAATAAAATCGCCGATTTAACCGACCGCACTTCCGTGCTTCGGGGGTATCTTTGACTTCGACGCCAAACTGGAGCGTTTAGAAGAAGTCAACGCCGAACTGGAACAGCCTGACGTATGGAACGATGCAGAGAAAGCGCAGGCGCTCGGCAAAGAACGCGTTTCTTTAGAAc                                <  1:541485/239‑1 (MQ=255)                                                                                                                                                                                                                   ttATAATCCCGCACTTAATTTACCTTTTTACGACATTCATTATCATGTTTGAAATTAATCCTATCAAAAATAAAATCGCCGATTTAACCGACCGCACTTCCGTGCTTCGGGGGTATCTTTGACTTCGACGCCAAACTGGAGCGTTTAGAAGAAGTCAACGCCGAACTGGAACAGCCTGACGTATGGAACGATGCAgaga                                                                       >  1:535011/1‑199 (MQ=255)                                                                                                                                                                                                                   ttATAATCCCGCACTTAATTTACCTTTTTACGACATTCATTATCATGTTTGAAATTAATCCTATCAAAAATAAAATCGCCGATTTAACCGACCGCACTTCCGTGCTTCGGGGGTATCTTTGACTTCGACGCCAAACTGGAGCGTTTAGAAGAAGTCAACGCCGAACTGGAACAGCCTGACGTATGGAACGATGCAGAGAAAGCGCAGGCGCTCGGCAAAGAACGCGTTTCTTTAGAACTGGTGGTCAacac                   <  1:73924/251‑1 (MQ=255)                                                                                                                                                                                                                   ttATAATCCCGCACTTAATTTACCTTTTTACGACATTCATTATCATGTTTGAAATTAATCCTATCAAAAATAAAATCGCCGATTTAACCGACCGCACTTCCGTGCTTCGGGGGTATCTTTGACTTCGACGCCAAACTGGAGCGTTTAGAAGAAGTCAACGCCGAACTGGAACAGCCTGACGTATGGAACGATGCAGAGAAAGCGCAGGCGCTCGGCAAAGAACGCGTTTCTTTAGAACTGGTGGTCAacac                   <  1:612525/251‑1 (MQ=255)                                                                                                                                                                                                                   ttATAATCCCGCACTTAATTTACCTTTTTACGACATTCATTATCATGTTTGAAATTAATCCTATCAAAAATAAAATCGCCGATTTAACCGACCGCACTTCCGTGCTTCGGGGGTATCTTTGACTTCGACGCCAAACTGGAGCGTTTAGAAGAAGTCAACGCCGAACTGGAACAGCCTGACGTATGGAACGATGCAGAGAAAGCGCAGGCGCTCGGCAAAGAACGCGTTTCTTTAGAACTGGTGGTCAaca                    >  1:751942/1‑250 (MQ=255)                                                                                                                                                                                                                        aTCCCGCACTTAATTTACCTTTTTACGACATTCATTATCATGTTTGAAATTAATCCTATCAAAAATAAAATCGCCGATTTAACCGACCGCACTTCCGTGCTTCGGGGGTATCTTTGACTTCGACGCCAAACTGGAGCGTTTagaag                                                                                                                       <  1:147219/146‑1 (MQ=255)                                                                                                                                                                                                                        aTCCCGCACTTAATTTACCTTTTTACGACATTCATTATCATGTTTGAAATTAATCCTATCAAAAATAAAATCGCCGATTTAACCGACCGCACTTCCGTGCTTCGGGGGTATCTTTGACTTCGACGCCAAACTGGAGCGTTTAGAAGAAGTCAACGCCGAACTGGAACAGCCTGACGTATGGaa                                                                                  >  1:91995/1‑183 (MQ=255)                                                                                                                                                                                                                           ccGCACTTAATTTACCTTTTTACGACATTCATTATCATGTTTGAAATTAATCCTATCAAAAATAAAATCGCCGATTTAACCGACCGCACTTCCGTGCTTCGGGGGTATCTTTGACTTCGACGCCAAACTGGAGCGTTTAGAAGAAGTCAACGCCGAACTGGAACAGCCTGACGTATGGAACGATGCAGAGAAAGCGCAGGCGCTCGGCAAAGAACGCGTTTCTTTAGAACTGGTGGTCAACACCATTaaaa           <  1:760371/251‑1 (MQ=255)                                                                                                                                                                                                                             gCACTTAATTTACCTTTTTACGACATTCATTATCATGTTTGAAATTAATCCTATCAAAAATAAAATCGCCGATTTAACCGACCGCACTTCCGTGCTTCGGGGGTATCTTTGACTTCGACGCCAAACTGGAGCGTTTAGaagaag                                                                                                                    >  1:262130/1‑144 (MQ=255)                                                                                                                                                                                                                              cACTTAATTTACCTTTTTACGACATTCATTATCATGTTTGAAATTAATCCTATCAAAAATAAAATCGCCGATTTAACCGACCGCACTTCCGTGCTTCGGGGGTATCTTTGACTTCGACGCCAAACTGGAGCGTTTAGAAGAAGTCAACGCCGAACTGGAACAGCCTGACGTATGGAACGATGCAGAGAAAGCGCAGGCGCTCGGCAAAGAACGCGTTTCTTTAGAACTGGTGGTCAACACCATTAAAAAt         >  1:899358/1‑250 (MQ=255)                                                                                                                                                                                                                              cACTTAATTTACCTTTTTACGACATTCATTATCATGTTTGAAATTAATCCTATCAAAAATAAAATCGCCGATTTAACCGACCGCACTTCCGTGCTTCGGGGGTATCTTTGACTTCGACGCCAAACTGGAGCGTTTAGAAGAAGTCAACGCCGAACTGGAACAGCCTGACGTATGGAACGATGCAGAGAAAGCGCAGGCGCTCGGCAAAGAACGCGTTTCTTTAGAACTGGTGGTCAACACCATTAAAAATc        <  1:152905/251‑1 (MQ=255)                                                                                                                                                                                                                                    aTTTACCTTTTTACGACATTCATTATCATGTTTGAAATTAATCCTATCAAAAATAAAATCGCCGATTTAACCGACCGCACTTCCGTGCTTCGGGGGTATCTTTGACTTCGACGCCAAACTGGAGCGTTTAGAAGAAGTCAACGCCGAACTGGAACAGCCTGACGTATGGAACGATGCAGAGAAAGCGCAGGCGCTCGGCAAAGAACGCGTTTCTTTAGAACTGGTGGTCAACACCATTAAAAATCTGGAAc  <  1:834813/251‑1 (MQ=255)                                                                                                                                                                                                                                    aTTTACCTTTTTACGACATTCATTATCATGTTTGAAATTAATCCTATCAAAAATAAAATCGCCGATTTAACCGACCGCACTTCCGTGCTTCGGGGGTATCTTTGACTTCGACGCCAAACTGGAGCGTTTAGAAGAAGTCAACGCCGAACTGGAACAGCCTGACGTATGGAACGATGCAGAGAAAGCGCAGGCGCTCGGCAAAGAACGCGTTTCTTTAGAACTGGTGGTCAACACCATTAAAAATCTGGAAc  <  1:227017/251‑1 (MQ=255)                                                                                                                                                                                                                                    aTTTACCTTTTTACGACATTCATTATCATGTTTGAAATTAATCCTATCAAAAATAAAATCGCCGATTTAACCGACCGCACTTCCGTGCTTCGGGGGTATCTTTGACTTCGACGCCAAACTGGAGCGTTTAGAAGAAGTCAACGCCGAACTGGAACAGCCTGACGTATGGAACGATGCAGAGAAAGCGCAGGCGCTCGGCAAAGAACGCGTTTCTTTAGAACTGGTGGTCAACACCATTAAAAATCTGGAAc  <  1:220570/251‑1 (MQ=255)                                                                                                                                                                                                                                    aTTTACCTTTTTACGACATTCATTATCATGTTTGAAATTAATCCTATCAAAAATAAAATCGCCGATTTAACCGACCGCACTTCCGTGCTTCGGGGGTATCTTTGACTTCGACGCCAAACTGGAGCGTTTAGAAGAAGTCAACGCCGAACTGGAACAGCCTGACGTATGGAACGATGCACAGAAAGCGCAGGCGCTCGGCAAAGAACGCGTTTCTTTAGAACTGGTGGTCAACACCATTAAAAATCTGGaa   >  1:169910/1‑250 (MQ=255)                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                           TTCCAATTTACTTTTCAGTGCAACATCATTTGCCGTTACACTCATAGATATAGCCAAAAGAGAAAGCGTAGTAATAATTTTTTTCATTTCTGTTGTTCCTAAATAAGTGAAATTGAGCGTGAATTATAAAAGTAATTGACGCGTTATGCAAAATTCACAGAATTTCACAGTTGGAGTTTCGTTCGCTCTACCCAATTCCCGCAAAATCCCTTATAATCCCGCACTTAATTTACCTTTTTACGAAATTCATTATCATGTTTGAAATTAATCCTATCAAAAATAAAATCGCCGATTTAACCGACCGCACTTCCGTGCTTCGGGGGTATCTTTGACTTCGACGCCAAACTGGAGCGTTTAGAAGAAGTCAACGCCGAACTGGAACAGCCTGACGTATGGAACGATGCAGAGAAAGCGCAGGCGCTCGGCAAAGAACGCGTTTCTTTAGAACTGGTGGTCAACACCATTAAAAATCTGGAAC  >  1/1539173‑1539650

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑