Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,535,903	T→C	intergenic (‑91275/‑105861)	unknown ← / → unknown	tRNA‑Met‑CAT/tRNA‑Gly‑GCC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,535,903	0	T	C	100.0%	181.3 / NA	66	intergenic (‑91275/‑105861)	unknown/unknown	tRNA‑Met‑CAT/tRNA‑Gly‑GCC
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (42/24);  total (42/24)

GCTTAATCTGCGCCAAACGTTCTCCGCCTTGAATAAAGCTATCGCCGGAACAAATTAAACCGCGTTTTACCCGTTGCCCAAGTTGTTTTGCAGTACGTTCCGCCAATTCGACCAAACGGGCATCAGAAACAAAAGTGACAGGGCATGCGGGTAGCTGTCCTTTGGCATAACCGAAGGCGGTCACATCGGCGTCGTGGTATTGGGTTTCGGTGGATACAATCACATCACCGACGTGCAATCCCTCTGCCACCCCACCGGCGGAACCTGTGTTAATCACCAGGTTCGGCTTAGCAAGTTGCAATAAAGCGGTGGTGCCCATGGCAGCAGCGACTTTGCCAATACCCGATTGCAACAAGGCAATATTTTTGCCATGAATTTTACCTTCATAAATCGTACCGGAAACCAATTTTGTGACGTTTTTATCCGTCATTAACCCAACCAAAATTTCCACTTCCTGCGCCATGGCGCCGACAATACCAATT  >  1/1535659‑1536140                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                              gCTTAATCTGCGCCAAACGTTCTCCGCCTTGAATAAAGCTATCGCCGGAACAAATTAAACCGCGTTTTACCCGTTGCCCAAGTTGTTTTGCAGTACGTTCCGCCAATTCGACCAAACGGGCATCAGAAACAAAAGTGACAGGGCATGCGGGTAGCTGTCCTTTGGCATAACCGAAGACGGTCACATCGGCGTCGTGGTATTGGGTTTCGGTGGATACAATCACATCACCGACGTGCAATCCCTCCGccac                                                                                                                                                                                                                                          >  1:169530/1‑250 (MQ=255) gCTTAATCTGCGCCAAACGTTCTCCGCCTTGAATAAAGCTATCGCCGGAACAAATTAAACCGCGTTTTACCCGTTGCCCAAGTTGTTTTGCAGTACGTTCCGCCAATTCGACCAAACGGGCATCAGAAACAAAAGTGACAGGGCATGCGGGTAGCTGTCCTTTGGCATAACCGAAGACGGTCACATCGGCGTCGTGGTATTGGGTTTCGGTGGATACAATCACATCACCGACGTGCAATCCCTCCGccac                                                                                                                                                                                                                                          >  1:606158/1‑250 (MQ=255)        cTGCGCCAAACGTTCTCCGCCTTGAATAAAGCTATCGCCGGAACAAATTAAACCGCGTTTTACCCGTTGCCCAAGTTGTTTTGCAGTACGTTCCGCCAATTCGACCAAACGGGCATCAGAAACAAAAGTGACAGGGCATGCGGGTAGCTGTCCTTTGGCATAACCGAAGACGGTCACATCGGCGTCGTGGTATTGGGTTTCGGTGGATACAATCACATCACCGACGTGCAATCCCTCCGCCACCCCACcgg                                                                                                                                                                                                                                  <  1:246021/251‑1 (MQ=255)                    ttCTCCGCCTTGAATAAAGCTATCGCCGGAACAAATTAAACCGCGTTTTACCCGTTGCCCAAGTTGTTTTGCAGTACGTTCCGCCAATTCGACCAAACGGGCATCAGAAACAAAAGTGACAGGGCATGCGGGTAGCTGTCCTTTGGCATAACCGAAGACGGTCACATCGGCGTCGTGGTATTGGGTTTCGGTGGATACAATCACATCACCGACGTGCAATCCCTCCGCCACCCCACCGGCGGAACCTgtgt                                                                                                                                                                                                                      <  1:261814/251‑1 (MQ=255)                         cGCCTTGAATAAAGCTATCGCCGGAACAAATTAAACCGCGTTTTACCCGTTGCCCAAGTTGTTTTGCAGTACGTTCCGCCAATTCGACCAAACGGGCATCAGAAACAAAAGTGACAGGGCATGCGGGTAGCTGTCCTTTGGCATAACCGAAGACGGTCACATCGGCGTCGTGGTATTGGGTTTCGGTGGATACAATCACATCACCGACGTGCAATCCCTCCGCCACCCCACCGGCGGAACCTGTGTTAATc                                                                                                                                                                                                                 <  1:946/251‑1 (MQ=255)                         cGCCTTGAATAAAGCTATCGCCGGAACAAATTAAACCGCGTTTTACCCGTTGCCCAAGTTGTTTTGCAGTACGTTCCGCCAATTCGACCAAACGGGCATCAGAAACAAAAGTGACAGGGCATGCGGGTAGCTGTCCTTTGGCATAACCGAAGACGGTCACATCGGCGTCGTGGTATTGGGTTTCGGTGGATACAATCACATCACCGACGTGCAATCCCTCCGCCACCCCACCGGCGGAACCTGTGTTAATc                                                                                                                                                                                                                 <  1:862663/251‑1 (MQ=255)                               gAATAAAGCTATCGCCGGAACAAATTAAACCGCGTTTTACCCGTTGCCCAAGTTGTTTTGCAGTACGTTCCGCCAATTCGACCAAACGGGCATCAGAAACAAAAGTGACAGGGCATGCGGGTAGCTGTCCTTTGGCATAACCGAAGACGGTCACATCGGCGTCGTGGTATTGGGTTTCGGTGGATACAATCACATCACCGACGTGCAATCCCTCCGCCACCCCACCGGCGGAACCTGTGTTAATCACCAgg                                                                                                                                                                                                           >  1:22124/1‑251 (MQ=255)                               gAATAAAGCTATCGCCGGAACAAATTAAACCGCGTTTTACCCGTTGCCCAAGTTGTTTTGCAGTACGTTCCGCCAATTCGACCAAACGGGCATCAGAAACAAAAGTGACAGGGCATGCGGGTAGCTGTCCTTTGGCATAACCGAAGACGGTCACATCGGCGTCGTGGTATTGGGTTTCGGTGGATACAATCACATCACCGACGTGCAATCCCTCCGCCACCCCACCGGCGGAACCTGTGTTAATCACCAgg                                                                                                                                                                                                           >  1:353048/1‑251 (MQ=255)                                       cTATCGCCGGAACAAATTAAACCGCGTTTTACCCGTTGCCCAAGTTGTTTTGCAGTACGTTCCGCCAATTCGACCAAACGGGCATCAGAAACAAAAGTGACAGGGCATGCGGGTAGCTGTCCTTTGGCATAACCGAAGACGGTCACATCGGCGTCGTGGTATTGGGTTTCGGTGGATACAATCACATCACCGACGTGCAATCCCTCCGCCACCCCACCGGCGGAACCTGTGTTAATCACCAGGTTCGGCtt                                                                                                                                                                                                   <  1:619888/251‑1 (MQ=255)                                           cGCCGGAACAAATTAAACCGCGTTTTACCCGTTGCCCAAGTTGTTTTGCAGTACGTTCCGCCAATTCGACCAAACGGGCATCAGAAACAAAAGTGACAGGGCATGCGGGTAGCTGTCCTTTGGCATAACCGAAGACGGTCACATCGGCGTCGTGGTATTGGGTTTCGGTGGATACAATCACATCACCGACGTGCAATCCCTCCGccac                                                                                                                                                                                                                                          >  1:395793/1‑208 (MQ=255)                                                    aaaTTAAACCGCGTTTTACCCGTTGCCCAAGTTGTTTTGCAGTACGTTCCGCCAATTCGACCAAACGGGCATCAGAAACAAAAGTGACAGGGCATGCGGGTAGCTGTCCTTTGGCATAACCGAAGACGGTCACATCGGCGTCGTGGTATTGGGTTTCGGTGGATACAATCACATCACCGACGTGCAATCCCTCCGCCACCCCACCGGCGGAACCTGTGTTAATCACCAGGTTCGGCTTAGCAAGTTGCAAt                                                                                                                                                                                      >  1:628437/1‑251 (MQ=255)                                                    aaaTTAAACCGCGTTTTACCCGTTGCCCAAGTTGTTTTGCAGTACGTTCCGCCAATTCGACCAAACGGGCATCAGAAACAAAAGTGACAGGGCATGCGGGTAGCTGTCCTTTGGCATAACCGAAGACGGTCACATCGGCGTCGTGGTATTGGGTTTCGGTGGATACAATCACATCACCGACGTGCAATCCCTCCGCCACCCCACCGGCGGAACCTGTGTTAATCACCAGGTTCGGCTTAGCAAGTTGCAAt                                                                                                                                                                                      >  1:283507/1‑251 (MQ=255)                                                     aaTTAAACCGCGTTTTACCCGTTGCCCAAGTTGTTTTGCTGTACGTTCCGCCAATTCGACCAAACGGGCATCAGAAACAAAAGTGACAGGGCATGCGGGTAGCTGTCCTTTGGCATAACCGAAGACGGTCACATCGGCGTCGTGGTATTGGGTTTCGGTGGATACAATCACATCACCGACGTGCAATCCCTCCGCCACCCCACCGGCGGAACCTGTGTTAATc                                                                                                                                                                                                                 >  1:208476/1‑223 (MQ=255)                                                     aaTTAAACCGCGTTTTACCCGTTGCCCAAGTTGTTTTGCAGTACGTTCCGCCAATTCGACCAAACGGGCATCAGAAACAAAAGTGACAGGGCATGCGGGTAGCTGTCCTTTGGCATAACCGAAGACGGTCACATCGGCGTCGTGGTATTGGGTTTCGGTGGATACAATCACATCACCGACGTGCAATCCCTCCGCCACCCCACCGGCGGAACCTGTGTTAATCACCAGGTTCGGCTTAGCAAGTTGCAATa                                                                                                                                                                                     <  1:605745/251‑1 (MQ=255)                                                               cgTTTTACCCGTTGCCCAAGTTGTTTTGCAGTACGTTCCGCCAATTCGACCAAACGGGCATCAGAAACAAAAGTGACAGGGCATGCGGGTAGCTGTCCTTTGGCATAACCGAAGACGGTCACATCGGCGTCGTGGTATTGGGTTTCGGTGGATACAATCACATCACCGACGTGCAATCCCTCCGcc                                                                                                                                                                                                                                            >  1:345197/1‑186 (MQ=255)                                                               cgTTTTACCCGTTGCCCAAGTTGTTTTGCAGTACGTTCCGCCAATTCGACCAAACGGGCATCAGAAACAAAAGTGACAGGGCATGCGGGTAGCTGTCCTTTGGCATAACCGAAGACGGTCACATCGGCGTCGTGGTATTGGGTTTCGGTGGATACAATCACATCACCGACGTGCAATCCCTCCGCCACCCCACCGGCGGAACCTGTGTTAATCACCAGGTTCGGCTTAGCAAGTTGCAATAAAGCggtggt                                                                                                                                                                           >  1:711691/1‑251 (MQ=255)                                                               cgTTTTACCCGTTGCCCAAGTTGTTTTGCAGTACGTTCCGCCAATTCGACCAAACGGGCATCAGAAACAAAAGTGACAGGGCATGCGGGTAGCTGTCCTTTGGCATAACCGAAGACGGTCACATCGGCGTCGTGGTATTGGGTTTCGGTGGATACAATCACATCACCGACGTGCAATCCCTCCGCCACCCCACCGGCGGAACCTGTGTTAATCACCAGGCTCGGCTTAGCAAGTTGCAATAAAGCggtggg                                                                                                                                                                           >  1:249326/1‑250 (MQ=255)                                                                                   ttgttTTGCAGTACGTTCCGCCAATTCGACCAAACGGGCATCAGAAACAAAAGTGACAGGGCATGCGGGTAGCTGTCCTTTGGCATAACCGAAGACGGTCACATCGGCGTCGTGGTATTGGGTTTCGGTGGATACAATCACATCACCGACGTGCAATCCCTCCGCCACCCCACCGGCGGAACCTGTGTTAATCACCAGGTTCGGCTTAGCAAGTTGCAATAAAGCGGTGGTGCCCATGGCAGCAGCGACt                                                                                                                                                        <  1:20057/250‑1 (MQ=255)                                                                                     gttTTGCAGTACGTTCCGCCAATTCGACCAAACGGGCATCAGAAACAAAAGTGACAGGGCATGCGGGTAGCTGTCCTTTGGCATAACCGAAGACGGTCACATCGGCGTCGTGGTATTGGGTTTCGGTGGATACAATCACATCACCGACGTGCAATCCCTCCGCCACCCCACCGGCGGAACCTGTGTTAATCACCAGGTTCGGCTTAGCAAGTTGCAATAAAGCGGTGGTGCCCATGGCAGCAGCGACTTTg                                                                                                                                                     >  1:213044/1‑251 (MQ=255)                                                                                        ttGCAGTACGTTCCGCCAATTCGACCAAACGGGCATCAGAAACAAAAGTGACAGGGCATGCGGGTAGCTGTCCTTTGGCATAACCGAAGACGGTCACATCGGCGTCGTGGTATTGGGTTTCGGTGGATACAATCACATCACCGACGTGCAATCCCTCCGCCACCCCACCGGCGGAACCTGTGTTAATCACCAGGTTCGGCTTAGCAAGTTGCAATAAAg                                                                                                                                                                                  <  1:721125/219‑1 (MQ=255)                                                                                        ttGCAGTACGTTCCGCCAATTCGACCAAACGGGCATCAGAAACAAAAGTGACAGGGCATGCGGGTAGCTGTCCTTTGGCATAACCGAAGACGGTCACATCGGCGTCGTGGTATTGGGTTTCGGTGGATACAATCACATCACCGACGTGCAATCCCTCCGCCACCCCACCGGCGGAACCTGTGTTAATCACCAGGTTCGGCTTAGCAAGTTGCAATAAAGCGGTGGTGCCCATGGCAGCAGCGACTTTGCCa                                                                                                                                                  >  1:49874/1‑251 (MQ=255)                                                                                        ttGCAGTACGTTCCGCCAATTCGACCAAACGGGCATCAGAAACAAAAGTGACAGGGCATGCGGGTAGCTGTCCTTTGGCATAACCGAAGACGGTCACATCGGCGTCGTGGTATTGGGTTTCGGTGGATACAATCACATCACCGACGTGCAATCCCTCCGCCACCCCACCGGCGGAACCTGTGTTAATCACCAGGTTCGGCTTAGCAAGTTGCAATAAAGCGGTGGTGCCCATGGCAGCAGCGACTTTGCCa                                                                                                                                                  >  1:747940/1‑251 (MQ=255)                                                                                             gTACGTTCCGCCAATTCGACCAAACGGGCATCAGAAACAAAAGTGACAGGGCATGCGGGTAGCTGTCCTTTGGCATAACCGAAGACGGTCACATCGGCGTCGTGGTATTGGGTTTCGGTGGATACAATCACATCACCGACGTGCAATCCCTCCGCCACCCCACCGGCGGAACCTGTGTTAATCACCAGGTTCGGCTTAGCAAGTTGCAATAAAGCGGTGGTGCCCATGGCAGCAGCGACTTTGCCAATAc                                                                                                                                              >  1:470377/1‑250 (MQ=255)                                                                                                    ccGCCAATTCGACCAAACGGGCATCAGAAACAAAAGTGACAGGGCATGCGGGTAGCTGTCCTTTGGCATAACCGAAGACGGTCACATCGGCGTCGTGGTATTGGGTTTCGGTGGATACAATCACATCACCGACGTGCAATCCCTCCGCCACCCCACCGGCGGAACCTGTGTTAATCACCAGGTTCGGCTTAGCAAGTTGCAATAAAGCGGTGGTGCCCATGGCAGCAGCGACTTTGCCAATACCCGATTg                                                                                                                                       >  1:702426/1‑250 (MQ=255)                                                                                                    ccGCCAATTCGACCAAACGGGCATCAGAAACAAAAGTGACAGGGCATGCGGGTAGCTGTCCTTTGGCATAACCGAAGACGGTCACATCGGCGTCGTGGTATTGGGTTTCGGTGGATACAATCACATCACCGACGTGCAATCCCTCCGCCACCCCACCGGCGGAACCTGTGTTAATCACCAGGTTCGGCTTAGCAAGTTGCAATAAAGCGGTGGTGCCCATGGCAGCAGCGACTTTGCCAATACCCGATTg                                                                                                                                       >  1:725438/1‑250 (MQ=255)                                                                                                         aaTTCGACCAAACGGGCATCAGAAACAAAAGTGACAGGGCATGCGGGTAGCTGTCCTTTGGCATAACCGAAGACGGTCACATCGGCGTCGTGGTATTGGGTTTCGGTGGATACAATCACATCACCGACGTGCAATCCCTCCGCCACCCCACCGGCGGAACCTGTGTTAATCACCAGGTTCGGCTTAGCAAGTTGCAATAAAGCGGTGGTGCCCATGGCAGCAGCGACTTTGCCAATACCCGATTGcaacaa                                                                                                                                 <  1:81821/251‑1 (MQ=255)                                                                                                            tCGACCAAACGGGCATCAGAAACAAAAGTGACAGGGCATGCGGGTAGCTGTCCTTTGGCATAACCGAAGACGGTCACATCGGCGTCGTGGTATTGGGTTTCGGTGGATACAATCACATCACCGACGTGCAATCCCTCCGCCACCCCACCGGCGGAACCTGTGTTAATCACCAGGTTCGGCTTAGCAAGTTGCAATAAAGCGGTGGTGCCCATGGCAGCAGCGACTTTGCCAATACCCGATTGCAACAAGGc                                                                                                                              <  1:243232/251‑1 (MQ=255)                                                                                                                             ggAAACAAAAGTGACAGGGCATGCGGGTAGCTGTCCTTTGGCATAACCGAAGACGGTCACATCGGCGTCGTGGTATTGGGTTTCGGTGGATACAATCACATCACCGACGTGCAATCCCTCCGCCACCCCACCGGCGGAACCTGTGTTAATCACCAGGtt                                                                                                                                                                                                         <  1:415690/158‑1 (MQ=255)                                                                                                                                  cAAAAGTGACAGGGCATGCGGGTAGCTGTCCTTTGGCATAACCGAAGACGGTCACATCGGCGTCGTGGTATTGGGTTTCGGTGGATACAATCACATCACCGACGTGCAATCCCTCCGCCACCCCACCGGCGGAACCTGTGTTAATCACCAGGTTCGGCTTAGCaa                                                                                                                                                                                              <  1:437874/165‑1 (MQ=255)                                                                                                                                  cAAAAGTGACAGGGCATGCGGGTAGCTGTCCTTTGGCATAACCGAAGACGGTCACATCGGCGTCGTGGTATTGGGTTTCGGTGGATACAATCACATCACCGACGTGCAATCCCTCCGCCACCCCACCGGCGGAACCTGTGTTAATCACCAGGTTCGGCTTAGCAAGTTGCAATAAAGCGGTGGTGCCCATGGCAGCAGCGACTTTGCCAATACCCGATTGCAACAAGGCAATATTTTTGCCATGAATTTTa                                                                                                        >  1:547057/1‑251 (MQ=255)                                                                                                                                     aaGTGACAGGGCATGCGGGTAGCTGTCCTTTGGCATAACCGAAGACGGTCACATCGGCGTCGTGGTATTGGGTTTCGGTGGATACAATCACATCACCGACGTGCAATCCCTCCGCCACCCCACCGGCGGAACCTGTGTTAATCACCAGGTTCGGCTTAGCAAGTTGCAATAAAGCGGTGGTGCCCATGGCAGCAGCGACTTTGCCAATAcc                                                                                                                                             <  1:6189/211‑1 (MQ=255)                                                                                                                                              ggCATGCGGGTAGCTGTCCTTTGGCATAACCGAAGACGGTCACATCGGCGTCGTGGTATTGGGTTTCGGTGGATACAATCACATCACCGACGTGCAATCCCTCCGCCACCCCACCGGCGGAACCTGTGTTa                                                                                                                                                                                                                    <  1:498172/131‑1 (MQ=255)                                                                                                                                               gCATGCGGGTAGCTGTCCTTTGGCATAACCGAAGACGGTCACATCGGCGTCGTGGTATTGGGTTTCGGTGGATACAATCACATCACCGACGTGCAATCCCTCCGCCACCCCACCGGCGGAACCTGTGTTAATCACCAGGTTCGGCTTAGCAAGTTGCAATAAAGCGGTGGTGCCCATGGCAGCAGCGACTTTGCCAATACCCGATTGCAACAAGGCAATATTTTTGCCATGAATTTTACCTTCATAAATc                                                                                            <  1:895998/250‑1 (MQ=255)                                                                                                                                                   gCGGGTAGCTGTCCTTTGGCATAACCGAAGACGGTCACATCGGCGTCGTGGTATTGGGTTTCGGTGGATACAATCACATCACCGACGTGCAATCCCTCCGCCACCCCACCGGCGGAACCTGTGTTAATCACCAGGTTCGGCTTAGCAAGTTGCAATAAAGCGGTGGGGCCCATGGCAGCAGCGACTTTGCCa                                                                                                                                                  >  1:770795/1‑192 (MQ=255)                                                                                                                                                     gggTAGCTGTCCTTTGGCATAACCGAAGACGGTCACATCGGCGTCGTGGTATTGGGTTTCGGTGGATACAATCACATCACCGACGTGCAATCCCTCCGCCACCCCACCGGCGGAACCTGTGTTAATCACCAGGTTCGGCTTAGCAAGTTGCAATAAAGCGGTGGTGCCCATGGCAGCAGCGACTTTGCCAATACCCGATTGCAACAAGGCAATATTTTTGCCATGAATTTTACCTTCATAAATCGTACCgg                                                                                     >  1:527475/1‑251 (MQ=255)                                                                                                                                                             gTCCTTTGGCATAACCGAAGACGGTCACATCGGCGTCGTGGTATTGGGTTTCGGTGGATACAATCACATCACCGACGTGCAATCCCTCCGCCACCCCACCGGCGGAACCTGTGTTAATCACCAGGTTCGGCTTAGCAAGTTGCAATAAAGCGGTGGTGCCCATGGCAGCAGCGACTTTGCCAATACCCGATTGCAACAAGGCAATATTTTTGCCATGAATTTTACCTTCATAAATCGTACCGGAAACCAAt                                                                             >  1:319538/1‑251 (MQ=255)                                                                                                                                                               ccTTTGGCATAACCGAAGACGGTCACATCGGCGTCGTGGTATTGGGTTTCGGTGGATACAATCACATCACCGACGTGCAATCCCTCCGCCACCCCACCGGCGGAACCTGTGTTAATCACCAGGTTCGGCTTAGCAAGTTGCAATAAAGCGGTGGTGCCCATGGCAGCAGCGACTTTGCCAATACCCGATTGCAACAAGGCAATATTTTTGCCATGAATTTTACCTTCATAAATCGTACCGGAAACCAAttt                                                                           >  1:724619/1‑251 (MQ=255)                                                                                                                                                                         aaCCGAAGACGGTCACATCGGCGTCGTGGTATTGGGTTTCGGTGGATACAATCACATCACCGACGTGCAATCCCTCCGCCACCCCACCGGCGGAACCTGTGTTAATCACCAGGTTCGGCTTAGCAAGTTGCAATAAAGCGGTGGTGCCCATGGCAGCAGCGACTTTGCCAATACCCGATTGCAACAAGGCAATATTTTTGCCATGAATTTTACCTTCATAAATCGTACCGGAAACCAATTTTGTGACGttt                                                                 >  1:891167/1‑251 (MQ=255)                                                                                                                                                                          aCCGAAGACGGTCGCATCGGCGTCGTGGTATTGGGTTTCGGTGGATACAATCACATCACCGACGTGCAATCCCTCCGCCACCCCACCGGCGGAACCTGTGTTAATCACCAGGTTCGGCTTAGCAAGTTGCAATAAAGCGGTGGTGCCCATGGCAGCAGCGACTTTGCCAATACCCGATTGCAACAAGGCAATATTTTTGCCATGAATTTTACCTTCATAAATCGTACCGGAAACCAATTTTGTGACGtttt                                                                >  1:841168/1‑251 (MQ=255)                                                                                                                                                                          aCCGAAGACGGTCACATCGGCGTCGTGGTATTGGGTTTCGGTGGATACAATCACATCACCGACGTGCAATCCCTCCGCCACCCCACCGGCGGAACCTGTGTTAATCACCAGGTTCGGCTTAGCAAGTTGCAATAAAGCGGTGGTGCCCATGGCAGCAGCGACTTTGCCAATACCCGATTGCAACAAGGCAATATTTTTGCCATGAATTTTACCTTCATAAATCGTACCGGAAACCAATTTTGTGACGtttt                                                                >  1:209884/1‑251 (MQ=255)                                                                                                                                                                          aCCGAAGACGGTCACATCGGCGTCGTGGTATTGGGTTTCGGTGGATACAATCACATCACCGACGTGCAATCCCTCCGCCACCCCACCGGCGGAACCTGTGTTAATCACCAGGTTCGGCTTAGCAAGTTGCAATAAAGCGGTGGTGCCCATGGCAGCAGCGACTTTGCCAATACCCGATTGCAACAAGGCAATATTTTTGCCATGAATTTTACCTTCATAAATCGTACCGGAAACCAATTTTGTGACGtttt                                                                >  1:762968/1‑251 (MQ=255)                                                                                                                                                                                gacGGTCACATCGGCGTCGTGGTATTGGGTTTCGGTGGATACAATCACATCACCGACGTGCAATCCCTCCGCCACCCCACCGGCGGAACCTGTGTTAATCACCAGGTTCGGCTTAGCAAGTTGCAATAAAGCGGTGGTGCCCATGGCAGCAGCGACTTTGCCAATACCCGATTGCAACAAGGCAATATTTTTGCCATGAATTTTACCTTCATAAATCGTACCGGAAACCAATTTTGTGACGTTTTTATCCg                                                          >  1:533519/3‑251 (MQ=255)                                                                                                                                                                                        caTCGGCGTCGTGGTATTGGGTTTCGGTGGATACAATCACATCACCGACGTGCAATCCCTCCGCCACCCCACCGGCGGAACCTGTGTTAATCACCAGGTTCGGCTTAGCAAGTTGCAATAAAGCGGTGGTGCCCATGGCAGCAGCGACTTTGCCAATACCCGATTGCAACAAGGCAATATTTTTGCCATGAATTTTACCTTCATAAATCGTACCGGAAACCAATTTTGTGACGTTTTTATCCGTCATTaa                                                   >  1:889861/1‑250 (MQ=255)                                                                                                                                                                                        caTCGGCGTCGTGGTATTGGGTTTCGGTGGATACAATCACATCACCGACGTGCAATCCCTCCGCCACCCCACCGGCGGAACCTGTGTTAATCACCAGGTTCGGCTTAGCAAGTTGCAATAAAGCGGTGGTGCCCATGGCAGCAGCGACTTTGCCAATACCCGATTGCAACAAGGCAATATTTTTGCCATGAATTTTACCTTCATAAATCGTACCGGAAACCAATTTTGTGACGTTTTTATCCGTCATTaa                                                   >  1:687998/1‑250 (MQ=255)                                                                                                                                                                                         aTCGGCGTCGTGGTATTGGGTTTCGGTGGATACAATCACATCACCGACGTGCAATCCCTCCGCCACCCCACCGGCGGAACCTGTGTTAATCACCAGGTTCGGCTTAGCAAGTTGCAATAAAGCGGTGGTGCCCATGGCAGCAGCGACTTTGCCAATACCCGATTGCAACAAGGCAATATTTTTGCCATGAATTTTACCTTCATAAATCGTACCGGAAACCAATTTTGTGACGTTTTTATCCGTCATTAAcc                                                 <  1:72774/251‑1 (MQ=255)                                                                                                                                                                                             gCGTCGTGGTATTGGGTTTCGGTGGATACAATCACATCACCGACGTGCAATCCCTCCGCCACCCCACCGGCGGAACCTGTGTTAATCACCAGGTTCGGCTTAGCAAGTTGCAATAAAGCGGTGGTGCCCATGGCAGCAGCGACTTTGCCAATACCCGATTGCAACAAGGCAATATTTTTGCCATGAATTTTACCTTCATAAATCGTACCGGAAACCAATTTTGTGACGTTTTTATCCGTCATTAAcc                                                 <  1:332198/247‑1 (MQ=255)                                                                                                                                                                                                  gtGGTATTGGGTTTCGGTGGATACAATCACATCACCGACGTGCAATCCCTCCGCCACCCCACCGGCGGAACCTGTGTTAATCACCAGGTTCGGCTTAGCAAGTTGCAATAAAGCGGTGGTGCCCATGGCAGCAGCGACTTTGCCAATACCCGATTGCAACAAGGCAATATTTTTGCCATGAATTTTACCTTCATAAATCGTACCGGAAACCAATTTTGTGACGTTTTTATCCGTCATTAACCCAACCaaaa                                        >  1:536447/1‑251 (MQ=255)                                                                                                                                                                                                   tGGTATTGGGTTTCGGTGGATACAATCACATCACCGACGTGCAATCCCTCCGCCACCCCACCGGCGGAACCTGTGTTAATCACCAGGTTCGGCTTAGCAAGTTGCAATAAAGCGGTGGTGCCCATGGCAGCAGCGACTTTGCCAATACCCGATTGCAACAAGGCAATATTTTTGCCATGAATTTTACCTTCATAAATCGTACCGGAAACCAATTTTGTGACGTTTTTATCCGTCATTAACCCAACCAAAAt                                       <  1:259956/251‑1 (MQ=255)                                                                                                                                                                                                     gTATTGGGTTTCGGTGGATACAATCACATCACCGACGTGCAATCCCTCCGCCACCCCACCGGCGGAACCTGTGTTAATCACCAGGTTCGGCTTAGCAAGTTGCAATAAAGCGGTGGTGCCCATGGCAGCAGCGACTTTGCCAATACCCGATTGCAACAAGGCAATATTTTTGCCATGAATTTTAc                                                                                                       >  1:138575/1‑185 (MQ=255)                                                                                                                                                                                                     gTATTGGGTTTCGGTGGATACAATCACATCACCGACGTGCAATCCCTCCGCCACCCCACCGGCGGAACCTGTGTTAATCACCAGGTTCGGCTTAGCAAGTTGCAATAAAGCGGTGGTGCCCATGGCAGCAGCGACTTTGCCAATACCCGATTGCAACAAGGCAATATTTTTGCCATGAATTTTACCTTCATAAATCGTACCGGAAACCAATTTTGTGACGTTTTTATCCGTCATTAACCCAACCAAAAttt                                     >  1:585196/1‑251 (MQ=255)                                                                                                                                                                                                     gTATTGGGTTTCGGTGGATACAATCACATCACCGACGTGCAATCCCTCCGCCACCCCACCGGCGGAACCTGTGTTAATCACCAGGTTCGGCTTAGCAAGTTGCAATAAAGCGGTGGTGCCCATGGCAGCAGCGACTTTGCCAATACCCGATTGCAAAAAGGCAATATTTTTGCCATGAATTTTACCTTCATAAATCGTACCGGAAACCAATTTTGTGACGTTTTTATCCGTCATTAACCCAACCAAACttt                                     >  1:452802/1‑251 (MQ=255)                                                                                                                                                                                                            gTTTCGGTGGATACAATCACATCACCGACGTGCAATCCCTCCGCCACCCCACCGGCGGAACCTGTGTTAATCACCAGGTTCGGCTTAGCAAGTTGCAATAAAGCGGTGGTGCCCATGGCAGCAGCGACTTTGcc                                                                                                                                                   >  1:349883/1‑134 (MQ=255)                                                                                                                                                                                                                   tGGATACAATCACATCACCGACGTGCAATCCCTCCGCCACCCCACCGGCGGAACCTGTGTTAATCACCAGGTTCGGCTTAGCAAGTTGCAATAAAGCGGTGGTGCCCATGGCAGCAGCGACTTTGCCAATACCCGATTGCAACAAGGCAATATTTTTGCCATGAATTTTACCTTCATAAATCGTACCGGAAACCAATTTTGTGACGTTTTTATCCGTCATTAACCCAACCAAAATTTCCACTTCCTGCGcc                       <  1:794354/251‑1 (MQ=255)                                                                                                                                                                                                                    ggATACAATCACATCACCGACGTGCAATCCCTCCGCCACCCCACCGGCGGAACCTGTGTTAATCACCAGGTTCGGCTTAGCAAGTTGCAATAAAGCGGTGGTGCCCATGGCAGCAGCGACTTTGCCAATACCCGATTGCAACAAGGCAATATTTTTGCCATGAATTTTACCTTCATAAATCGTACCGGAAACCAATTTTGTGACGTTTTTATCCGTCATTAACCCAACCAAAATTTCCAc                                 <  1:365116/240‑1 (MQ=255)                                                                                                                                                                                                                     gATACAATCACATCACCGACGTGCAATCCCTCCGCCACCCCACCGGCGGAACCTGTGTTAATCACCAGGTTCGGCTTAGCAAGTTGCAATAAAGCGGTGGTGCCCATGGCAGCAGCGACTTTGCCAATACCCGATTGCAACAAGGCAATATTTTTGCCATGAATTTTACCTTCATAAATCGTACCGGAAACCAATTTTGTGACGTTTTTATCCGTCATTAACCCAACCAAAATTTCCACTTCCTGCGCCAt                     >  1:438963/1‑251 (MQ=255)                                                                                                                                                                                                                      gtACAATCCCCTCCCCGACGTGCAATCCCTCCGCCCCCCCACCGGCGGAACCTGTGTTAATCACCAGGTTCGGCTTAGCAAGTTGCAATAAAGCGGTTGTGCCCCTGGCAGCAGCGACTTTGCCAATTCCCGATTGCAACAAGGCAATATTTTTGCCATGAATTTTACCTTCATAAATCGTACCGGAAACCAATTTTGTGACGTTTTTATCCGTCATTAACCCAACCAAAATTTCCACTTCCTGCGcc                       <  1:496540/247‑1 (MQ=255)                                                                                                                                                                                                                      aTACAATCACATCACCGACGTGCAATCCCTCCGCCACCCCACCGGCGGAACCTGTGTTAATCACCAGGTTCGGCTTAGCAAGTTGCAATAAAGCGGTGGTGCCCATGGCAGCAGCGACTTTGCCAATACCCGATTGCAACAAGGCAATATTTTTGCCATGAATTTTACCTTCATAAATCGTACCGGAAACCAATTTTGTGACGTTTTTATCCGTCATTAACCCAACCAAAATTTCCACTTCCTGCGcc                       <  1:390674/248‑1 (MQ=255)                                                                                                                                                                                                                         cAATCACATCACCGACGTGCAATCCCTCCGCCACCCCACCGGCGGAACCTGTGTTAATCACCAGGTTCGGCTTAGCAAGTTGCAATAAAGCGGTGGTGCCCATGGCAGCAGCGACTTTGCCAATACCCGATTGCAACAAGGCAATATTTTTGCCATGAATTTTACCTTCATAAATCGTACCGGAAACCAATTTTGTGACGTTTTTATCCGTCATTAACCCAACCAAAATTTCCACTTCCTGCGCCATGgcg                 >  1:776404/1‑251 (MQ=255)                                                                                                                                                                                                                               catcacCGACGTGCAATCCCTCCGCCACCCCACCGGCGGAACCTGTGTTAATCACCAGGTTCGGCTTAGCAAGTTGCAATAAAGCGGTGGTGCCCATGGCAGCAGCGACTTTGCCAATACCCGATTGCAACAAGGCAATATTTTTGCCATGAATTTTACCTTCATAAATCGTACCGGAAACCAATTTTGTGACGTTTTTATCCGTCATTAACCCAACCAAAATTTCCACTTCCTGCGCCATGgcgc                <  1:492671/246‑1 (MQ=255)                                                                                                                                                                                                                               catcacCGACGTGCAATCCCTCCGCCACCCCACCGGCGGAACCTGTGTTAATCACCAGGTTCGGCTTAGCAAGTTGCAATAAAGCGGTGGTGCCCATGGCAGCAGCGACTTTGCCAATACCCGATTGCAACAAGGCAATATTTTTGCCATGAATTTTACCTTCATAAATCGTACCGGAAACCAATTTTGTGACGTTTTTATCCGTCATTAACCCAACCAAAATTTCCACTTCCTGCGCCATGGCGCCGACa           >  1:183674/1‑251 (MQ=255)                                                                                                                                                                                                                               catcacCGACGTGCAATCCCTCCGCCACCCCACCGGCGGAACCTGTGTTAATCACCAGGTTCGGCTTAGCAAGTTGCAATAAAGCGGTGGTGCCCATGGCAGCAGCGACTTTGCCAATACCCGATTGCAACAAGGCAATATTTTTGCCATGAATTTTACCTTCATAAATCGTACCGGAAACCAATTTTGTGACGTTTTTATCCGTCATTAACCCAACCAAAATTTCCACTTCCTGCGCCATGGCGCCGACa           >  1:723471/1‑251 (MQ=255)                                                                                                                                                                                                                               catcacCGACGTGCAATCCCTCCGCCACCCCACCGGCGGAACCTGTGTTAATCACCAGGTTCGGCTTAGCAAGTTGCAATAAAGCGGTGGTGCCCATGGCAGCAGCGACTTTGCCAATACCCGATTGCAACAAGGCAATATTTTTGCCATGAATTTTACCTTCATAAATCGTACCGGAAACCAATTTTGTGACGTTTTTATCCGTCATTAACCCAACCAAAATTTCCACTTCCTGCGCCATGGCGCCGACa           >  1:238562/1‑251 (MQ=255)                                                                                                                                                                                                                               catcacCGACGTGCAATCCCTCCGCCACCCCACCGGCGGAACCTGTGTTAATCACCAGGTTCGGCTTAGCAAGTTGCAATAAAGCGGTGGTGCCCATGGCAGCAGCGACTTTGCCAATACCCGATTGCAACAAGGCAATATTTTTGCCATGAATTTTACCTTCATAAATCGTACCGGAAACCAATTTTGTGACGTTTTTATCCGTCATTAACCCAACCAAAATTTCCACTTCCTGCGCCATGGCGCCGACa           >  1:562504/1‑251 (MQ=255)                                                                                                                                                                                                                                   acCGACGTGCAATCCCTCCGCCACCCCACCGGCGGAACCTGTGTTAATCACCAGGTTCGGCTTAGCAAGTTGCAATAAAGCGGTGGTGCCCATGGCAGCAGCGACTTTGCCAATACCCGATTGCAACAAGGCAATATTTTTGCCATGAATTTTACCTTCATAAATCGTACCGGAAACCAATTTTGTGACGTTTTTATCCGTCATTAACCCAACCAAAATTTCCACTTCCTGCGCCATGGCGCCGACAATa        >  1:532950/1‑250 (MQ=255)                                                                                                                                                                                                                                       aCGTGCAATCCCTCCGCCACCCCACCGGCGGAACCTGTGTTAATCACCAGGTTCGGCTTAGCAAGTTGCAATAAAGCGGTGGTGCCCATGGCAGCAGCGACTTTGCCAATACCCGATTGCAACAAGGCAATAtt                                                                                                                        >  1:291017/1‑134 (MQ=255)                                                                                                                                                                                                                                        cGTGCAATCCCTCCGCCACCCCACCGGCGGAACCTGTGTTAATCACCAGGTTCGGCTTAGCAAGTTGCAATAAAGCGGTGGTGCCCATGGCAGCAGCGACTTTGCCAATACCCGATTGCAACAAGGCAATATTTTTGCCATGAATTTTACCTTCATAAATCGTACCGGAAACCAATTTTGTGACGTTTTTATCCGTCATTAACCCAACCAAAATTTCCACTTCCTGCGCCATGGCGCCGACAATACCAAtt  <  1:764526/251‑1 (MQ=255)                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                              GCTTAATCTGCGCCAAACGTTCTCCGCCTTGAATAAAGCTATCGCCGGAACAAATTAAACCGCGTTTTACCCGTTGCCCAAGTTGTTTTGCAGTACGTTCCGCCAATTCGACCAAACGGGCATCAGAAACAAAAGTGACAGGGCATGCGGGTAGCTGTCCTTTGGCATAACCGAAGGCGGTCACATCGGCGTCGTGGTATTGGGTTTCGGTGGATACAATCACATCACCGACGTGCAATCCCTCTGCCACCCCACCGGCGGAACCTGTGTTAATCACCAGGTTCGGCTTAGCAAGTTGCAATAAAGCGGTGGTGCCCATGGCAGCAGCGACTTTGCCAATACCCGATTGCAACAAGGCAATATTTTTGCCATGAATTTTACCTTCATAAATCGTACCGGAAACCAATTTTGTGACGTTTTTATCCGTCATTAACCCAACCAAAATTTCCACTTCCTGCGCCATGGCGCCGACAATACCAATT  >  1/1535659‑1536140

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑