Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,519,779	A→C	intergenic (‑75151/‑121985)	unknown ← / → unknown	tRNA‑Met‑CAT/tRNA‑Gly‑GCC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,519,779	0	A	C	100.0%	183.5 / NA	67	intergenic (‑75151/‑121985)	unknown/unknown	tRNA‑Met‑CAT/tRNA‑Gly‑GCC
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (25/42);  total (25/42)

GCATCATCCATTTGCCTTTGGTATTGCCCCCTGTGGTGATTGGTTACC‑‑‑TGTTGTTGGTGGCAATGGGACGCAACGGTTTCATCGGCAAATATTTATACCAATGGTTCGGGCTGACTTTCGGCTTCAGCTGGCGCGGGGCGGTATTAGCCGCTGCCGTGGTGGCATTTCCATTAGTGGTTCGGGCAATTCGTCTTTCGCTGGAAAGTATCGACTTAAAATTGGAACAAGCCGCCAGAACCTTGGGAGCTTCCCCTTGGCGGGTATTTTTTACCATCACCTTGCCCCTATCTTTGCCGGGAATTTTAGCGGGCATCGTATTGGGATTTGCCCGCTCCTTGGGTGAATTCGGCGCAACCATTACTTTCGTTTCCAATATCGCCGGTGAAACACAAACTATTCCTTTAGCAATGTATTCTTTCATTCAAACGCCCGGCGCGGAAGAACAAGCCGCCCGTTTATGTGTTTTTGCTATAATTTTATCCCTGGTTT  >  1/1519535‑1520023                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                     gCATCATCCATTTGCCTTTGGTATTGCCCCCTGTGGTGATTGGTTACC‑‑‑TGTTGTTGGTGGCAATGGGACGCAACGGTTTCATCGGCAAATATTTATACCAATGGTTCGGGCTGACTTTCGGCTTCAGCTGGCGCGGGGCGGTATTAGCCGCTGCCGTGGTGGCATTTCCATTAGTGGTTCGGGCAATTCGTCTTTCGCTGGAAAGTATCGACTTAAAATTGGAACAAGCCGCCAGAACCTTGGGCGCTTc                                                                                                                                                                                                                                                 >  1:723136/1‑250 (MQ=255)        ccATTTGCCTTTGGTATTGCCCCCTGTGGTGATTGGTTACC‑‑‑TGTTGTTGGTGGCAATGGGACGCAACGGTTTCATCGGCAAATATTTATACCAATGGTTCGGGCTGACTTTCGGCTTCAGCTGGCGCGGGGCGGTATTAGCCGCTGCCGTGGTGGCATTTCCATTAGTGGTTCGGGCAATTCGTCTTTCGCTGGACAGTATCGACTTAACATTGGAACAAGCCGCCAGAACCTTGGGCGCTTCCCCTTgg                                                                                                                                                                                                                                          >  1:745447/1‑250 (MQ=255)            ttGCCTTTGGTATTGCCCCCTGTGGTGATTGGTTACCTGTTGTTGTTGGTGGCAATGGGACGCAACGGTTTCATCGGCAAATATTTATACCAATGGTTCGGGCTGACTTTCGGCTTCAGCTGGCGCGGGGCGGTATTAGCCGCTGCCGTGGTGGCATTTCCATTAGTGGTTCGGGCAATTCGTCTTTCGCTGGAAAGTATCGACTTAAAATTGGAACAAGCCGCCAGAACCTTGGGCGCTTCCCCTTGGCg                                                                                                                                                                                                                                        <  1:139631/251‑1 (MQ=255)               ccTTTGGTATTGCCCCCTGTGGTGATTGGTTACC‑‑‑TGTTGTTGGTGGCAATGGGACGCAACGGTTTCATCGGCAAATATTTATACCAATGGTTCGGGCTGACTTTCGGCTTCAGCTGGCGCGGGGCGGTATTAGCCGCTGCCGTGGTGGCATTTCCATTAGTGGTTCGGGCAATTCGTCTTTCGCTGGAAAGTATCGACTTAAAATTGGAACAAGCCGCCAGAACCTTGGGCGCTTCCCCTTGGCGGGTAtt                                                                                                                                                                                                                                  >  1:306530/1‑251 (MQ=255)                     gTATTGCCCCCTGTGGTGATTGGTTACC‑‑‑TGTTGTTGGTGGCAATGGGACGCAACGGTTTCATCGGCAAATATTTATACCAATGGTTCGGGCTGACTTTCGGCTTCAGCTGGCGCGGGGCGGTATTAGCCGCTGCCGTGGTGGCATTTCCATTAGTGGTTCGGGCAATTCGTCTTTCGCTGGAAAGTATCGACTTAAAATTGGAACAAGCCGCCAGAACCTTGGGCGCTTCCCCTTGGCg                                                                                                                                                                                                                                        >  1:308220/1‑239 (MQ=255)                      tATTGCCCCCTGTGGTGATTGGTTACC‑‑‑TGTTGTTGGTGGCAATGGGACGCAACGGTTTCATCGGCAAATATTTATACCAATGGTTCGGGCTGACTTTCGGCTTCAGCTGGCGCGGGGCGGTATTAGCCGCTGCCGTGGTGGCATTTCCATTAGTGGTTCGGGCAATTCGTCTTTCGCTGGAAAGTATCGACTTAAAATTGGAACAAGCCGCCAGAACCTTGGGCGCTTCCCCTTGGCGGGTATTTTTTAc                                                                                                                                                                                                                            <  1:792527/250‑1 (MQ=255)                          gCCCCCTGTGGTGATTGGTTACC‑‑‑TGTTGTTGGTGGCAATGGGACGCAACGGTTTCATCGGCAAATATTTATACCAATGGTTCGGGCTGACTTTCGGCTTCAGCTGGCGCGGGGCGGTATTAGCCGCTGCCGTGGTGGCATTTCCATTAGTGGTTCGGGCAATTCGTCTTTCGCTGGAAAGTATCGACTTAAAATTGGAACAAGCCGCCAGAACCTTGGGCGCTTCCCCTTGGc                                                                                                                                                                                                                                         <  1:574124/233‑1 (MQ=255)                           cccccTGTGGTGATTGGTTACC‑‑‑TGTTGTTGGTGGCAATGGGACGCAACGGTTTCATCGGCAAATATTTATACCAATGGTTCGGGCTGACTTTCGGCTTCAGCTGGCGCGGGGCGGTATTAGCCGCTGCCGTGGTGGCATTTCCATTAGTGGTTCGGGCAATTCGTCTTTCGCTGGAAAGTATCGACTTAAAATTGGAACAAGCCGCCAGAACCTTGGGCGCTTCCCCTTGGCGGGTATTTTTTACCATCa                                                                                                                                                                                                                       >  1:114633/1‑250 (MQ=255)                            ccccTGTGGTGATTGGTTACC‑‑‑TGTTGTTGGTGGCAATGGGACGCAACGGTTTCATCGGCAAATATTTATACCAATGGTTCGGGCTGACTTTCGGCTTCAGCTGGCGCGGGGCGGTATTAGCCGCTGCCGTGGTGGCATTTCCATTAGTGGTTCGGGCAATTCGTCTTTCGCTGGAAAGTATCGACTTAAAATTGGAACAAGCCGCCAGAACCTTGGGCGCTTCCCCTTGGCGGGTATTTTTTACCATCAc                                                                                                                                                                                                                      >  1:725385/1‑250 (MQ=255)                               cTGTGGTGATTGGTTACC‑‑‑TGTTGTTGGTGGCAATGGGACGCAACGGTTTCATCGGCAAATATTTATACCAATGGTTCGGGCTGACTTTCGGCTTCAGCTGGCGCGGGGCGGTATTAGCCGCTGCCGTGGTGGCATTTCCATTAGTGGTTCGGGCAATTCGTCTTTCGCTGGAAAGTATCGACTTAAAATTGGAACAAGCCGCCAGAACCTTGGGCGCTTCCCCTTGGCGGGGATTTTTTACCATCAccctt                                                                                                                                                                                                                  >  1:47190/1‑248 (MQ=255)                                 gtggtgATTGGTTACC‑‑‑TGTTGTTGGTGGCAATGGGACGCAACGGTTTCATCGGCAAATATTTATACCAATGGTTCGGGCTGACTTTCGGCTTCAGCTGGCGCGGGGCGGTATTAGCCGCTGCCGTGGTGGCATTTCCATTAGTGGTTCGGGCAATTCGTCTTTCGCTGGAAAGTATCGACTTAAAATTGGAACAAGCCGCCAGAACCTTGGGCGCTTCCCCTTGGCGGGGATTTTTTACCATCACCTTGc                                                                                                                                                                                                                 >  1:267299/1‑250 (MQ=255)                                  tggtgATTGGTTCCC‑‑‑TGTTGTTGGTGGCAATGGGACGCAACGGTTTCATCGGCAAATATTTATACCAATGGTTCGGGCTGACTTTCGGCTTCAGCTGGCGCGGGGCGGTATTAGCCGCTGCCGTGGTGGCATTTCCATTAGTGGTTCGGGCAATTCGTCTTTCGCTGGAAAGTATCGACTTAAAATTGGAACAAGCCGCCAGAACCTTGGGCGCTTCCCCTTGGCGGGTATTTTTTACCATCACCTTGccc                                                                                                                                                                                                               <  1:770452/251‑1 (MQ=255)                                       aTTGGTTACC‑‑‑TGTTGTTGGTGGCAATGGGACGCAACGGTTTCATCGGCAAATATTTATACCAATGGTTCGGGCTGACTTTCGGCTTCAGCTGGCGCGGGGCGGTATTAGCCGCTGCCGTGGTGGCATTTCCATTAGTGGTTCGGGCAATTCGTCTTTCGCTGGAAAGTATCGACTTAAAATTGGAACAAGCCGCCAGAACCTTGGGCGCTTCCCCTTGGCGGGTATTTTTTACCATCACCTTGCCCCTAt                                                                                                                                                                                                           <  1:614541/250‑1 (MQ=255)                                       aTTGGTTACC‑‑‑TGTTGTTGGTGGCAATGGGACGCAACGGTTTCATCGGCAAATATTTATACCAATGGTTCGGGCTGACTTTCGGCTTCAGCTGGCGCGGGGCGGTATTAGCCGCTGCCGTGGTGGCATTTCCATTAGTGGTTCGGGCAATTCGTCTTTCGCTGGAAAGTATCGACTTAAAATTGGAACAAGCCGCCAGAACCTTGGGCGCTTCCCCTTGGCGGGTATTTTTTACCATCACCTTGCCCCTATc                                                                                                                                                                                                          <  1:273153/251‑1 (MQ=255)                                       aTTGGTTACC‑‑‑TGTTGTTGGTGGCAATGGGACGCAACGGTTTCATCGGCAAATATTTATACCAATGGTTCGGGCTGACTTTCGGCTTCAGCTGGCGCGGGGCGGTATTAGCCGCTGCCGTGGTGGCATTTCCATTAGTGGTTCGGGCAATTCGTCTTTCGCTGGAAAGTATCGACTTAAAATTGGAACAAGCCGCCAGAACCTTGGGCGCTTCCCCTTGGCGGGTATTTTTTACCATCACCTTGCCCCTATc                                                                                                                                                                                                          <  1:288865/251‑1 (MQ=255)                                       aTTGGTTACC‑‑‑TGTTGTTGGTGGCAATGGGACGCAACGGTTTCATCGGCAAATATTTATACCAATGGTTCGGGCTGACTTTCGGCTTCAGCTGGCGCGGGGCGGTATTAGCCGCTGCCGTGGTGGCATTTCCATTAGTGGTTCGGGCAATTCGTCTTTCGCTGGAAAGTATCGACTTAAAATTGGAACAAGCCGCCAGAACCTTGGGCGCTTCCCCTTGGCGGGTATTTTTTACCATCACCTTGCCCCTATc                                                                                                                                                                                                          <  1:320512/251‑1 (MQ=255)                                                                  aTGGGACGCAACGGTTTCATCGGCAAATATTTATACCAATGGTTCGGGCTGACTTTCGGCTTCAGCTGGCGCGGGGCGGTATTAGCCGCTGCCGTGGTGGCATTTCCATTAGTGGTTCGGGCAATTCGTCTTTCGCTGGAAAGTATCGACTTAAAATTGGAACAAGCCGCCAGAACCTTGGGCGCTTCCCCTTGGCGGGTATTTTTTACCATCACCTTGCCCCTATCTTTGCCGGGAATTTTAGCGGGCAt                                                                                                                                                                                  >  1:317507/1‑251 (MQ=255)                                                                          cAACGGTTTCATCGGCAAATATTTATACCAATGGTTCGGGCTGACTTTCGGCTTCAGCTGGCGCGGGGCGGTATTAGCCGCTGCCGTGGTGGCATTTCCATTAGTGGTTCGGGCAATTCGTCTTTCGCTGGAAAGTATCGACTTAAAATTGGAACAAGCCGCCAGAACCTTGGGCGCTTCCCCTTGGCGGGTATTTTTTACCATCACCTTGCCCCTATCTTTGCCGGGAATTTTAGCGGGCATCGTATTgg                                                                                                                                                                          <  1:811669/251‑1 (MQ=255)                                                                            aCGGTTTCATCGGCAAATATTTATACCAATGGTTCGGGCTGACTTTCGGCTTCAGCTGGCGCGGGGCGGTATTAGCCGCTGCCGTGGTGGCATTTCCATTAGTGGTTCGGGCAATTCGTCTTTCGCTGGAAAGTATCGACTTAAAATTGGAACAAGCCGCCAGAACCTTGGGCGCTTCCCCTTGGCGGGTATTTTTTACCATCACCTTGCCCCTATCTTTGCCGGGAATTTTAGCGGGCATCGTATTGGGa                                                                                                                                                                        <  1:669814/251‑1 (MQ=255)                                                                            aCGGTTTCATCGGCAAATATTTATACCAATGGTTCGGGCTGACTTTCGGCTTCAGCTGGCGCGGGGCGGTATTAGCCGCTGCCGTGGTGGCATTTCCATTAGTGGTTCGGGCAATTCGTCTTTCGCTGGAAAGTATCGACTTAAAATTGGAACAAGCCGCCAGAACCTTGGGCGCTTCCCCTTGGCGGGTATTTTTTACCATCACCTTGCCCCTATCTTTGCCGGGAATTTTAGCGGGCATCGTATTGGGa                                                                                                                                                                        <  1:568865/251‑1 (MQ=255)                                                                            aCGGTTTCATCGGCAAATATTTATACCAATGGTTCGGGCTGACTTTCGGCTTCAGCTGGCGCGGGGCGGTATTAGCCGCTGCCGTGGTGGCATTTCCATTAGTGGTTCGGGCAATTCGTCTTTCGCTGGAAAGTATCGACTTAAAATTGGAACAAGCCGCCAGAACCTTGGGCGCTTCCCCTTGGCGGGTATTTTTTACCATCACCTTGCCCCTATCTTTGCCGGGAATTTTAGCGGGCATCGTATTGGGa                                                                                                                                                                        <  1:770941/251‑1 (MQ=255)                                                                            aCGGTTTCATCGGCAAATATTTATACCAATGGTTCGGGCTGACTTTCGGCTTCAGCTGGCGCGGGGCGGTATTAGCCGCTGCCGTGGTGGCATTTCCATTAGTGGTTCGGGCAATTCGTCTTTCGCTGGAAAGTATCGACTTAAAATTGGAACAAGCCGCCAGAACCTTGGGCGCTTCCCCTTGGCGGGTATTTTTTACCATCACCTTGCCCCTATCTTTGCCGGGAATTTTAGCGGGCATCGTATTGGGa                                                                                                                                                                        <  1:813467/251‑1 (MQ=255)                                                                            aCGGTTTCATCGGCAAATATTTATACCAATGGTTCGGGCTGACTTTCGGCTTCAGCTGGCGCGGGGCGGTATTAGCCGCTGCCGTGGTGGCATTTCCATTAGTGGTTCGGGCAATTCGTCTTTCGCTGGAAAGTATCGACTTAAAATTGGAACAAGCCGCCAGAACCTTGGGCGCTTCCCCTTGGCGGGTATTTTTTACCATCACCTTGCCCCTATCTTTGCCGGGAATTTTAGCGGGCATCGTATTGGGa                                                                                                                                                                        <  1:529763/251‑1 (MQ=255)                                                                            aCGGTTTCATCGGCAAATATTTATACCAATGGTTCGGGCTGACTTTCGGCTTCAGCTGGCGCGGGGCGGTATTAGCCGCTGCCGTGGTGGCATTTCCATTAGTGGTTCGGGCAATTCGTCTTTCGCTGGAAAGTATCGACTTAAAATTGGAACAAGCCGCCAGAACCTTGGGCGCTTCCCCTTGGCGGGTATTTTTTACCATCACCTTGCCCCTATCTTTGCCGGGAATTTTAGCGGGCATCGTATTGGGa                                                                                                                                                                        <  1:229401/251‑1 (MQ=255)                                                                             cGGTTTCATCGGCAAATATTTATCCCCATGGTTCGGGCTGGCTTTCGGCTTCAGCTGGCGCGGGGCGGTATTAGCCGCTGCAGTGGTGGCATTTCCATTAGTGGTTCGGGCAATTCGTCTTTCGCTGGAAAGTATCGACTTAAAATTGGAACAAGCCGACAGAACCTTGGGCGCTTCCCCTTGGCGGGTATTTTTTACCATCACCTTGCCCCTATCTTTGCCGGGAATTTTAGCGGGCATCGTATTGGGAt                                                                                                                                                                       <  1:670542/251‑1 (MQ=255)                                                                             cGGTTTCATCGGCAAATATTTATACCAATGGTTCGGGCTGACTTTCGGCTTCAGCTGGCGCGGGGCGGTATTAGCCGCTGCCGTGGTGGCATTTCCATTAGTGGTTCGGGCAATTCGTCTTTCGCTGGAAAGTATCGACTTAAAATTGGAACAAGCCGCCAGAACCTTGGGCGCTTCCCCTTGGCGGGTATTTTTTACCATCACCTTGCCCCTATCTTTGCCGGGAATTTTAGCGGGCATCGTATTGGGAt                                                                                                                                                                       <  1:267927/251‑1 (MQ=255)                                                                             cGGTTTCATCGGCAAATATTTATACCAATGGTTCGGGCTGACTTTCGGCTTCAGCTGGCGCGGGGCGGTATTAGCCGCTGCCGTGGTGGCATTTCCATTAGTGGTTCGGGCAATTCGTCTTTCGCTGGAAAGTATCGACTTAAAATTGGAACAAGCCGCCAGAACCTTGAGCGCTTCCCCTTGGCGGGTATTTTTTACCATCACCTTGCCCCTATCTTTGCCGGGAATTTTTGCGGGCATCGTATTGGGa                                                                                                                                                                        >  1:428268/1‑250 (MQ=255)                                                                                tttCATCGGCAAATATTTATTCCCATGGTTCGGGCTGGCTTTCGGCTTCAGCTGGCGCGGGGCGGTATTAGCCGCTGGCGTGGTGGCATTTCCATTAGTGGTTCGGGCAATTCGTCTTTCGCTGGAAAGTATCGACTTAAAATTGGAACAAGCCGCCAGAACCTTGGGCGCTTCCCCTTGGCGGGTATTTTTTACCATCACCTTGCCCCTATCTTTGCCGGGAATTTTAGCGGGCATCGTATTGGGATTTg                                                                                                                                                                    <  1:668713/251‑1 (MQ=255)                                                                                            atatTTATACCAATGGTTCGGGCTGACTTTCGGCTTCAGCTGGCGCGGGGCGGTATTAGCCGCTGCCGTGGTGGCATTTCCATTAGTGGTTCGGGCAATTCGTCTTTCGCTGGAAAGTATCGACTTAAAATTGGAACAAGCCGCCAGAACCTTGGGCGCTTCCCCTTGGCGGGGATTTTTTACCATCACCTTGCCCCTATCTTTGCCGGGGATTTTAGCGGGCCTCGTATTGGGATTTGCCCGCCCCTTgg                                                                                                                                                        >  1:720689/1‑251 (MQ=255)                                                                                             tatTTATACCAATGGTTCGGGCTGACTTTCGGCTTCAGCTGGCGCGGGGCGGTATTAGCCGCTGCCGTGGTGGCATTTCCATTAGTGGTTCGGGCAATTCGTCTTTCGCTGGAAAGTATCGACTTAAAATTGGAACAAGCCGCCAGAACCTTGGGCGCTTCCCCTTGGCGGGTATTTTTTACCATCACCTTGCCCCTATCTTTGCCGGGAATTTTAGCGGGCATCGTATTggg                                                                                                                                                                         <  1:671502/233‑1 (MQ=255)                                                                                             tatTTATACCAATGGTTCGGGCTGACTTTCGGCTTCAGCTGGCGCGGGGCGGTATTAGCCGCTGCCGTGGTGGCATTTCCATTAGTGGTTCGGGCAATTCGTCTTTCGCTGGAAAGTATCGACTTAAAATTGGAACAAGCCGCCAGAACCTTGGGCGCTTCCCCTTGGCGGGTATTTTTTACCATCACCTTGCCCCTATCTTTGCCGGGAATTTTAGCGGGCATCGTATTGGGa                                                                                                                                                                        <  1:491682/234‑1 (MQ=255)                                                                                             tatTTATACCAATGGTTCGGGCTGACTTTCGGCTTCAGCTGGCGCGGGGCGGTATTAGCCGCTGCCGTGGTGGCATTTCCATTAGTGGTTCGGGCAATTCGTCTTTCGCTGGAAAGTATCGACTTAAAATTGGAACAAGCCGCCAGAACCTTGGGCGCTTCCCCTTGGCGGGTATTTTTTACCATCACCTTGCCCCTATCTTTGCCGGGAATTTTAGCGGGCATCGTATTGGGATTTGCCCGCTCCTTggg                                                                                                                                                       <  1:322321/251‑1 (MQ=255)                                                                                              atTTATTCCAATGGTTCGGGCTGACTTTCGGCTTCAGCTGGCGCGGGGCGGTATTAGCCGCTGGCGTTGTGGGATTTCCATTAGTGGTTCGGGCAATTCGTCTTTCGCTGGAAAGTATCGACTTAAAATTGGAACAAGCCGCCAGAACCTTGGGCGCTTCCCCTTGGCGGGTATTTTTTACCATCAcc                                                                                                                                                                                                                     <  1:64894/188‑1 (MQ=255)                                                                                              atTTATACCAATGGTTCGGGCTGACTTTCGGCTTCAGCTGGCGCGGGGCGGTATTAGCCGCTGCCGTGGTGGCATTTCCATTAGTGGTTCGGGCAATTCGTCTTTCGCTGGAAAGTATCGACTTAAAATTGGAACAAGCCGCCAGAACCTTGGGCGCTTCCCCTTGGCGGGGATTTTTTACCATCACCTTGccc                                                                                                                                                                                                               >  1:147022/1‑194 (MQ=255)                                                                                                                  cTGACTTTCGGCTTCAGCTGGCGCGGGGCGGTATTAGCCGCTGCCGTGGTGGCATTTCCATTAGTGGTTCGGGCAATTCGTCTTTCGCTGGAAAGTATCGACTTAAAATTGGAACAAGCCGCCAGAACCTTGGGCGCTTCCCCTTGGCGGGTATTTTTTACCATCACCTTGCCCCTATCTTTGCCGGGAATTTTAGCGGGCATCGTATTGGGATTTGCCCGCTCCTTGGGTGAATTCGGCGCAACCATTAc                                                                                                                                  <  1:827306/251‑1 (MQ=255)                                                                                                                                    tGGCGCGGGGCGGTATTAGCCGCTGCCGTGGTGGCATTTCCATTAGTGGTTCGGGCAATTCGTCTTTCGCTGGAAAGTATCGACTTAAAATTGGAACAAGCCGCCAGAACCTTGGGCGCTTCCCCTTGGCGGGTATTTTTTACCATCACCTTGCCCCTATCTTTGCCGGGAATTTTAGCGGGCATCGTATTGGGATTTGCCCGCTCCTTGGGTGAATTCGGCGCAACCATTACTTTCGTTTCCAATATCGc                                                                                                                <  1:170724/251‑1 (MQ=255)                                                                                                                                               ggTATTAGCCGCTGCCGTGGTGGCATTTCCATTAGTGGTTCGGGCAATTCGTCTTTCGCTGGAAAGTATCGACTTAAAATTGGAACAAGCCGCCAGAACCTTGGGCGCTTCCCCTTGGCGGGTATTTTTTACCATCCCCTTGCCCCTATCTTTGCCGGGAATTTTAGCGGGCATCGTATTGGGATTTGCCCGCTCCCTTGGTGAATTCGGCGCAACCATTTCTTTCGTTTCCAATATCCCCGGTGAAaca                                                                                                      >  1:464965/1‑250 (MQ=255)                                                                                                                                                gTATTAGCCGCTGCCGTGGTGGCATTTCCATTAGTGGTTCGGGCAATTCGTCTTTCGCTGGAAAGTATCGACTTAAAATTGGAACAAGCCGCCAGAACCTTGGGCGCTTCCCCTTGGCGGGTATTTTTTACCATCACCTTGCCCCTATCTTTGCCGGGAATTTTAGCGGGCATCGTATTGGGATTTGCCCGCTCCTTGGGTGAATTCGGCGCAACCATTACTTTCGTTTCCAATATCGCCg                                                                                                              <  1:458955/241‑1 (MQ=255)                                                                                                                                                gTATTAGCCGCTGCCGTGGTGGCATTTCCATTAGTGGTTCGGGCAATTCGTCTTTCGCTGGAAAGTATCGACTTAAAATTGGAACAAGCCGCCAGAACCTTGGGCGCTTCCCCTTGGCGGGGATTTTTTACCATCACCTTGCCCCTATCTTTGCCGGGAATTTTAGCGGGCATCGTATTGGGATTTGCCCGCTCCTTGGGTGAATTCGGCGCAACCATTACTTTCGTTTCCAATATCGCCGGTGAAAcaca                                                                                                    >  1:141565/1‑251 (MQ=255)                                                                                                                                                    tAGCCGCTGCCGTGGTGGCATTTCCATTAGTGGTTCGGGCAATTCGTCTTTCGCTGGAAAGTATCGACTTAAAATTGGAACAAGCCGCCAGAACCTTGGGCGCTTCCCCTTGGCGGGTATTTTTTACCATCACCTTGCCCCTATCTTTGCCGGGAATTTTAGCGGGCATCGTATTGGGATTTGCCCGCTCCTTGGGTGAATTCGGCGCAACCATTACTTTCGTTTCCAATATCGCCGGTGAAACACAAACt                                                                                                <  1:213397/251‑1 (MQ=255)                                                                                                                                                     aGCCGCTGCCGTGGTGGCATTTCCATTAGTGGTTCGGGCAATTCGTCTTTCGCTGGAAAGTATCGACTTAAAATTGGAACAAGCCGCCAGAACCTTGGGCGCTTCCCCTTGGCGGGTATTTTTTACCATCACCTTGCCCCTATCTTTGc                                                                                                                                                                                                     <  1:596235/149‑1 (MQ=255)                                                                                                                                                          cTGCCGTGGTGGCATTTCCATTAGTGGTTCGGGCAATTCGTCTTTCGCTGGAAAGTATCGACTTAAAATTGGAACAAGCCGCCAGAACCTTGGGCGCTTCCCCTTGGCGGGTATTTTTTACCATCACCTTGCCCCTATCTTTGCCGGGAATTTTAGCGGGCATCGTATTGGGATTTGCCCGCTCCTTGGGTGAATTCGGCGCAACCATTACTTTCGTTTCCAATATCGCCGGTGAAACACAAACTATTcc                                                                                           <  1:801548/250‑1 (MQ=255)                                                                                                                                                             ccGTGGTGGCATTTCCATTAGTGGTTCGGGCAATTCGTCTTTCGCTGGAAAGTATCGACTTAAAATTGGAACAAGCCGCCAGAACCTTGGGCGCTTCCCCTTGGCGGGTATTTTTTACCATCACCTTGCCCCTATCTTTGCCGGGAATTTTAGCGGGCATCGTATTGGGATTTGCCCGCTCCTTGGGTGAATTCGGCGCAACCATTACTTTCGTTTCCAATATCGCCGGTGAAACACAAACTATTCCttt                                                                                        <  1:399011/250‑1 (MQ=255)                                                                                                                                                                tggtggCATTTCCATTAGTGGTTCGGGCAATTCGTCTTTCGCTGGAAAGTATCGACTTAAAATTGGAACAAGCCGCCAGAACCTTGGGCGCTTCCCCTTGGCGGGTATTTTTTACCATCACCTTGCCCCTATCTTTGCCGGGAATTTTAGCGGGCATCGTATTGGGATTTGCCCGCTCCTTGGGTGAATTCGGCGCAACCATTACTTTCGTTTCCAATATCGCCGGTGAAACACAAACTATTCCTTTAGc                                                                                     <  1:486152/250‑1 (MQ=255)                                                                                                                                                                  gtggCATTTCCATTAGTGGTTCGGGCAATTCGTCTTTCGCTGGAAAGTATCGACTTAAAATTGGAACAAGCCGCCAGAACCTTGGGCGCTTCCCCTTGGCGGGTATTTTTTACCATCACCTTGCCCCTATCTTTGCCGGGAATTTTAGCGGGCATCGTATTGGGATTTGCCCGCTCCTTGGGTGAATTCGGCGCAACCATTACTTTCGTTTCCAATATCGCCGGTGAAACACAAACTATTCCTTTAGCaa                                                                                   <  1:151283/250‑1 (MQ=255)                                                                                                                                                                    ggCATTTCCATTAGTGGTTCGGGCAATTCGTCTTTCGCTGGAAAGTATCGACTTAAAATTGGAACAAGCCGCCAGAACCTTGGGCGCTTCCCCTTGGCGGGTATTTTTTACCATCACCTTGCCCCTATCTTTGCCGGGAATTTTAGCGGGCATCGTATTGGGATTTGCCCGCTCCTTGGGTGAATTCGGCGCAACCATTACTTTCGTTTCCAATATCGCCGGTGAAACACAAACTATTCCTTTAGCAAttt                                                                                >  1:194987/1‑249 (MQ=255)                                                                                                                                                                      cATTTCCATTAGTGGTTCGGGCAATTCGTCTTTCGCTGGAAAGTATCGACTTAAAATTGGAACAAGCCGCCAGAACCTTGGGCGCTTCCCCTTGGCGGGTATTTTTTACCATCACCTTGCCCCTATCTTTGCCGGGAATTTTAGCGGGCATCGTATTGGGATTTGCCCGCTCCTTGGGTGAATTCGGCGCAACCATTACTTTCGTTTCCAATATCGCCGGTGAAACACAAACTATTCCTTTAGCAATGTAt                                                                              <  1:635356/251‑1 (MQ=255)                                                                                                                                                                         ttCCATTAGTGGTTCGGGCAATTCGTCTTTCGCTGGAAAGTATCGACTTAAAATTGGAACAAGCCGCCAGAACCTTGGGCGCTTCCCCTTGGCGGGTATTTTTTACCATCACCTTGCCCCTATCTTTGCCGGGAATTTTAGCGGGCATCGTATTgg                                                                                                                                                                          >  1:760011/1‑156 (MQ=255)                                                                                                                                                                         ttCCATTAGTGGTTCGGGCAATTCGTCTTTCGCTGGAAAGTATCGACTTAAAATTGGAACAAGCCGCCAGAACCTTGGGCGCTTCCCCTTGGCGGGTATTTTTTACCATCACCTTGCCCCTATCTTTGCCGGGAATTTTAGCGGGCATCGTATTGGGATTTGCCCGCTCCTTGGGTGAATTCGGCGCAACCATTACTTTCGTTTCCAATATCGCCGGTGAAACACAAACTATTCCTTTAGCAATGTATTCt                                                                           <  1:538365/251‑1 (MQ=255)                                                                                                                                                                           ccATTAGTGGTTCGGGCAATTCGTCTTTCGCTGGAAAGTATCGACTTAAAATTGGAACAAGCCGCCAGAACCTTGGGCGCTTCCCCTTGGCGGGTATTTTTTACCATCACCTTGCCCCTATCTTTGCCGGGAATTTTAGCGGGCATCGTATTGGGATTTGCCCGCTCCTTGGGTGAATTCGGCGCAACCATTACTTTCGTTTCCAATATCGCCGGTGAAACACAAACTATTCCTTTAGCAATGTATTCttt                                                                         <  1:202414/251‑1 (MQ=255)                                                                                                                                                                            cATTAGTGGTTCGGGCAATTCGTCTTTCGCTGGAAAGTATCGACTTAAAATTGGAACAAGCCGCCAGAACCTTGGGCGCTTCCCCTTGGCGGGTATTTTTTACCATCACCTTGCCCCTATCTTTGCCGGGAATTTTAGCGGGCATCGTATTGGGATTTGCCCGCTCCTTGGGGGAATTCGGCGCAACCATTACTTTCGTTTCCAATATCGCCGGTGAAACACAAACTATTCCTTTAGCAATGTATTCttt                                                                         >  1:654200/1‑250 (MQ=255)                                                                                                                                                                            cATTAGTGGTTCGGGCAATTCGTCTTTCGCTGGAAAGTATCGACTTAAAATTGGAACAAGCCGCCAGAACCTTGGGCGCTTCCCCTTGGCGGGGATTTTTTACCATCACCTTGCCCCTATCTTTGCCGGGAATTTTAGCGGGCAACGTATTGGGATTTGCCCGCTCCTTGGGGGAATTCGGCGCAACCATTCCTTTCGTTTCCAATATCGCCGGTGAAACACAAACTATTCCtt                                                                                         >  1:323336/1‑234 (MQ=255)                                                                                                                                                                                  tGGTTCGGGCAATTCGTCTTTCGCTGGAAAGTATCGACTTAAAATTGGAACAAGCCGCCAGAACCTTGGGCGCTTCCCCTTGGCGGGTATTTTTTACCATCACCTTGCCCCTATCTTTGCCGGGAATTTTAGCGGGCATCGTATTGGGATTTGCCCGCTCCTTGGGTGAATTCGGCGCAACCATTACTCTCGTTTCCAATATCGCCGGTGAAACACAAACTATTCCTTTAGCAATGTATTCTttcattc                                                                    <  1:62633/249‑1 (MQ=255)                                                                                                                                                                                                     ttCGCTGGAAAGTATCGACTTAAAATTGGAACAAGCCGCCAGAACCTTGGGCGCTTCCCCTTGGCGGGTATTTTTTACCATCACCTTGCCCCTATCTTTGCCGGGAATTTTAGCGGGCATCGTATTGGGATTTGCCCGCTCCTTGGGTGAATTCGGCGCAACCATTACTTTCGTTTCCAATATCGCCGGTGAAACACAAACTATTCCTTTAGCAATGTATTCTTTCATTCAAACGCCCGGCGCGGAAGAAc                                               <  1:554319/251‑1 (MQ=255)                                                                                                                                                                                                       cGCTGGAAAGTATCGACTTAAAATTGGAACAAGCCGCCAGAACCTTGGGCGCTTCCCCTTGGCGGGTATTTTTTACCATCACCTTGCCCCTATCTTTGCCGGGAATTTTAGCGGGCATCGTATTGGGATTTGCCCGCTCCTTGGGTGAATTCGGCGCAACCATTACTTTCGTTTCCAATATCGCCGGTGAAACACAAACTATTCCTTTAGCAATGTATTCTTTCATTCAAACGCCCGGCGCGGAAGAACaa                                             >  1:326735/1‑251 (MQ=255)                                                                                                                                                                                                            gAAAGTATCGACTTAAAATTGGAACAAGCCGCCAGAACCTTGGGCGCTTCCCCTTGGCGGGTATTTTTTACCATCACCTTGCCCCTATCTTTGCCGGGAATTTTAGCGGGCATCGTATTGGGATTTGCCCGCTCCTTGGGTGAATTCGGCGCAACCATTACTTTCGTTTCCAATATCGCCGGTGAAACACAAACTATTCCTTTAGCAATGTATTCTTTCATTCAAACGCCCGGCGCGGAAGAACAAgccg                                         <  1:258711/250‑1 (MQ=255)                                                                                                                                                                                                                gTATCGACTTAAAATTGGAACAAGCCGCCAGAACCTTGGGCGCTTCCCCTTGGCGGGTATTTTTTACCATCACCTTGCCCCTATCTTTGCCGGGAATTTTAGCGGGCATCGTATTGGGa                                                                                                                                                                        <  1:486739/119‑1 (MQ=255)                                                                                                                                                                                                                   tCGACTTAAAATTGGAACAAGCCGCCAGAACCTTGGGCGCTTCCCCTTGGCGGGTATTTTTTACCATCACCTTGCCCCTATCTTTGCCGGGAATTTTAGCGGGCATCGTATTGGGATTTGCCCGCTCCTTGGGTGAATTCGGCGCAACCATTACTTTCGTTTCCAATATCGCCGGTGAAACACAAACTATTCCTTTAGCAATGTATTCTTTCATTCAAACGCCCGGCGCGGAAGAACAAGCCGCCCGTTTa                                 <  1:571279/251‑1 (MQ=255)                                                                                                                                                                                                                               tGGAACAAGCCGCCAGAACCTTGGGCGCTTCCCCTTGGCGGGTATTTTTTACCATCACCTTGCCCCTATCTTTGCCGGGAATTTTAGCGGGCATCGTATTGGGATTTGCCCGCTCCTTGGGTGAATTCGGCGCAACCATTACTTTCGTTTCCAATATCGCCGGTGAAACACAAACTATTCCTTTAGCAATGTATTCTTTCATTCAAACGCCCGGCGCGGAAGAACAAGCCGCCCGTTTATGTGTTTTTGCt                     <  1:262238/251‑1 (MQ=255)                                                                                                                                                                                                                               tGGAACAAGCCGCCAGAACCTTGGGCGCTTCCCCTTGGCGGGTATTTTTTACCATCACCTTGCCCCTATCTTTGCCGGGAATTTTAGCGGGCATCGTATTGGGATTTGCCCGCTCCTTGGGTGAATTCGGCGCAACCATTACTTTCGTTTCCAATATCGCCGGTGAAACACAAACTATTCCTTTAGCAATGTATTCTTTCATTCAAACGCCCGGCGCGGAAGAACAAGCCGCCCGTTTATGTGTTTTTGCt                     <  1:770311/251‑1 (MQ=255)                                                                                                                                                                                                                               tGGAACAAGCCGCCAGAACCTTGGGCGCTTCCCCTTGGCGGGTATTTTTTACCATCACCTTGCCCCTATCTTTGCCGGGAATTTTAGCGGGCATCGTATTGGGATTTGCCCGCTCCTTGGGTGAATTCGGCGCAACCATTACTTTCGTTTCCAATATCGCCGGTGAAACACAAACTATTCCTTTAGCAATGTATTCTTTCATTCAAACGCCCGGCGCGGAAGAACAAGCCGCCCGTTTATGTGTTTTTGCt                     <  1:181822/251‑1 (MQ=255)                                                                                                                                                                                                                                          gccAGAACCTTGGGCGCTTCCCCTTGGCGGGTATTTTTTACCATCACCTTGCCCCTATCTTTGCCGGGAATTTTAGCGGGCATCGTATTGGGATTTGcc                                                                                                                                                                  >  1:620383/1‑99 (MQ=255)                                                                                                                                                                                                                                                aCCTTGGGCGCTTCCCCTTGGCGGGTATTTTTTACCATCACCTTGCCCCTATCTTTGCCGGGAATTTTAGCGGGCATCGTATTGGGATTTGCCCGCTCCTTGGGTGAATTCGGCGCAACCATTACTTTCGTTTCCAATATCGCCGGTGAAACACAAACTATTCCTTTAGc                                                                                     >  1:370149/1‑170 (MQ=255)                                                                                                                                                                                                                                                 ccTTGGGCGCTTCCCCTTGGCGGGTATTTTTTACCATCACCTTGCCCCTATCTTTGCCGGGAATTTTAGCGGGCATCGTATTGGGATTTGCCCGCTCCTTGGGTGAATTCGGCGCAACCATTACTTTCGTTTCCAATATCGCCGGTGAAACACAAACTATTCCTTTAGc                                                                                     >  1:849218/1‑169 (MQ=255)                                                                                                                                                                                                                                                 ccTTGGGCGCTTCCCCTTGGCGGGGATTTTTTACCATCACCTTGCCCCTATCTTTGCCGGGAATTTTAGCGGGCATCGTATTGGGATTTGCCCGCTCCTTGGGGGAATTCGGCGCAACCATTACTTTCGTTTCCAATATCGCCGGTGAAACACAAACTATTCCTTTAGCAATGTATTCTTTCATTCAAACGCCCGGCGCGGAAGAACAAGCCGCCCGTTTATGTGTTTTTGCTATAATTTTATCCCTgggt   >  1:614196/1‑249 (MQ=255)                                                                                                                                                                                                                                                 ccTTGGGCGCTTCCCCTTGGCGGGGATTTTTTACCATCACCTTGCCCCTATCTTTGCCGGGAATTTTAGCGGGCATCGTATTGGGATTTGCCCGCTCCTTGGGGGAATTCGGCGCAACCATTACTTTCGTTTCCAATATCGCCGGTGAAACACAAACTATTCCTTTAGCAATGTATTCTTTCATTCAAACGCCCGGCGCGGAAGAACAAGCCGCCCGTTTATGTGTTTTTGCTATAATTTTATCCCTGGtt   >  1:531245/1‑251 (MQ=255)                                                                                                                                                                                                                                                  cTTGGGCGCTTCCCCTTGGCGGGTATTTTTTACCATCACCTTGCCCCTATCTTTGCCGGGAATTTTAGCGGGCATCGTATTGGGATTTGCCCGCTCCTTGGGTGAATTCGGCGCAACCATTACTTTCGTTTCCAATATCGCCGGTGAAACACAAACTATTCCTTTAGCAATGTATTCTTTCATTCAAACGCCCGGCGCGGAAGAACAAGCCGCCCGTTTATGTGTTTTTGCTATAATTTTATCCCTGGttt  >  1:634705/1‑251 (MQ=255)                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                     GCATCATCCATTTGCCTTTGGTATTGCCCCCTGTGGTGATTGGTTACC‑‑‑TGTTGTTGGTGGCAATGGGACGCAACGGTTTCATCGGCAAATATTTATACCAATGGTTCGGGCTGACTTTCGGCTTCAGCTGGCGCGGGGCGGTATTAGCCGCTGCCGTGGTGGCATTTCCATTAGTGGTTCGGGCAATTCGTCTTTCGCTGGAAAGTATCGACTTAAAATTGGAACAAGCCGCCAGAACCTTGGGAGCTTCCCCTTGGCGGGTATTTTTTACCATCACCTTGCCCCTATCTTTGCCGGGAATTTTAGCGGGCATCGTATTGGGATTTGCCCGCTCCTTGGGTGAATTCGGCGCAACCATTACTTTCGTTTCCAATATCGCCGGTGAAACACAAACTATTCCTTTAGCAATGTATTCTTTCATTCAAACGCCCGGCGCGGAAGAACAAGCCGCCCGTTTATGTGTTTTTGCTATAATTTTATCCCTGGTTT  >  1/1519535‑1520023

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑